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Wyniki wyszukiwania dla: social represeantations of biotechnology
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Rural Landscape Planning for Sustainable Development. The Case of Poland
PublikacjaThis text results from theoretical study on the possibilities of rural landscape renovation in Poland. The introduction marks new spatial phenomena and the scope of their influence. It indicates social and cultural reasons of the changes, and states the need of their renewed interpretation. The second part of the article contains a description of the degrading cultural landscape of the country and presents its features. It then...
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Application of the near-infrared spectroscopy in the pharmaceutical technology
PublikacjaNear-infrared (NIR) spectroscopy is currently the fastest-growing and the most versatile analytical method not only in the pharmaceutical sciences but also in the industry. This review focuses on recent NIR applications in the pharmaceutical technology. This article covers monitoring, by NIR, of many manufacturing processes, such as granulation, mixing or drying, in order to determine the end-point of these processes. In this...
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Electoral support for extreme right-wing parties: A sub-national analysis of western European elections
PublikacjaThis paper addresses two major limitations of cross-national research on electoral support for extreme right parties (ERPs) in western Europe: its almost exclusive focus on national-level data and its failure to examine the role of the social welfare state and social capital. We employ Tobit I estimations in an additive and interactive model and compute conditional coefficients and standard errors for several interactive variables....
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Application of magnetic nanoparticles for magnetic solid-phase extraction in preparing biological, environmental and food samples
PublikacjaThe need to obtain meaningful results as the basis for determining the content of trace amounts of analytes has become the driving force behind the development of modern analytical techniques, including sample-preparation techniques, such as solid-phase extraction (SPE). Recently, great interest was aroused in the use of magnetic nanoparticles (MNPs) in SPE. These materials exhibit high selectivity, and, in small amounts, can provide...
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Online brand communities’ contribution to digital business models
PublikacjaAbstract Purpose – There is limited research examining social drivers and mediators of online brand community identification in the context of business models development. This study aims to identify them behind the social mechanisms and present essential factors which should be applied in business models to foster value co-creation. Design/methodology/approach – Data were collected from a convenience sample of 712 cases gathered among...
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The Third Sector in the Universities’ Third Mission
PublikacjaThe interactions between the today’s university and its environment, as part of the university’s social responsibility, have been subject to many scientific papers. Their authors typically focus on interdependencies between universities, the state and the market. What is lacking are publications related to the interactions between universities and third sector organisations, and more broadly – a civil society, that may play an...
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Child-Robot Interaction Studies During COVID-19 Pandemic
PublikacjaThe coronavirus disease (COVID-19) pandemic affected our lives deeply, just like everyone else, the children also suffered from the restrictions due to COVID-19 affecting their education and social interactions with others, being restricted from play areas and schools for a long time. Although social robots provide a promising solution to support children in their education, healthcare, and social interaction with others, the precautions...
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The concept of aida applied to online interactive advertisement: an youtube case study
PublikacjaThis paper presents an approach of application of the AIDA framework to interactive advertisements presented on social media channels. The first section introduces the definitions of social media and its categorization. It is given an overview of the online video service YouTube.com. The second section describes social media marketing. The third section presents a theoretical introduction of traditional and interactive advertisement....
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Stardust - Investigation of Microbes in the Stratosphere
PublikacjaThe stratospheric microbiome has been investigated several times using the methods of classical microbiology. In this experiment, we have combined them with some novel approaches including whole- metagenome amplification, Maldi TOF mass spectrometry and Sanger DNA sequencing. The results of the experiment may provide the scientists with knowledge about the mechanisms of survivability of microorganisms in stratospheric conditions...
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A place of school in the idea of the sustainable development
PublikacjaWell designed school space enables social contacts, increases activity of pupils and improves the condition of their health. However, the best design would not bring the expected benefits if it is not integrated with the surroundings in terms of transportation, security and local needs. In the sustainable development, the social goals are in harmony with ecological aims according to the rule of "3 r" (reduce, reuse, recycle).
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CSR in Family Business- Ethnocentric Meaning Socially Responsible?
PublikacjaThe aim of this paper is to provide framework and key issues relevant for research on social responsibility of family business. From providing two theoretical approaches towards social responsibility such as social capital concept commenting on the familiness as a family firm resource and capability; and the stakeholder theory we move to the state of the art of family business. Then, we discuss heterogeneity among family firms...
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Discovery and characterization of RecA protein of thermophilic bacterium Thermus thermophilus MAT72 phage Tt72 that increases specificity of a PCR-based DNA amplification
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A bifunctional chimeric protein consisting of MutS and beta-galactoside
PublikacjaPraca pokazuje konstrukcję DNA plazmidowego kodującego bifunkcjonalne białko chimeryczne złożone z MutS Thermus thermophilus i beta-galaktozydazy E.coli, optymalizację jego ekspresji i oczyszczania. Białko to testowano w metodzie wykrywania mutacji punktowych, wykorzystując kolorymetryczne mierzenie aktywności domeny reporterowej beta galaktozydazy.
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Reaction of different microorganisms to novel graphene-based nanomaterials
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Construction and Use of a Broad-Host-Range Plasmid Expressing the lamB Gene for Utilization of Bacteriophage λ Vectors in the Marine Bacterium Vibrio harveyi
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Rapid eradication of bacterial phytopathogens by atmospheric pressure glow discharge generated in contact with a flowing liquid cathode
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Isolation and characterization of the restriction endonucleasePpel fromPhormidium persicinum
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Enhanced extraction of proteins using cholinium-based ionic liquids as phase-forming components of aqueous biphasic systems
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Hot spot identification by ligand-protein surface interactions mapping – in silico study
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Software tools for identification, visualization and analysis of protein tunnels and channels
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Evolution of functionally important compartments of proteins – what can we learn from studying epoxide hydrolases?
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Water tracing as an alternative method for tunnels exploration in macromolecules
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A bifunctional chimeric protein consisting of MutS and beta-galactosidase
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The construction of bifunctional fusion proteins consisting of MutS and GFP
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Bacterial SSB-like proteins of family Deinococcaceae - indentification, isolation, obtaining, purification and molecular characteristic
PublikacjaWiększość poznanych białek SSB jest aktywna w formie homotetramerycznej. Od 2002 roku znane są białka organizmów ekstremofilnych. Scharakteryzowano białka mikroorganizmów rodziny Deinococaceae. Uzyskane termostabilne białka stanowią atrakcyjną alternatywę w stosunku do znanych białek SSB w metodach diagnostyki molekularnej, technikach biologii molekularnej i analityce.
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A dependency between PRL gene polymorphism and breeding parameters in Polish Black-and-White Holstein-Friesian cows
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Direct cloning of heavy metal resistance genes from metagenomic DNA
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Effect of addition of buserelin acetate to the extender on motility and viability of bovine spermatozoa
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Correlation between chosen polymorphisms in genes coding 5HTT, 5HT2A and COMT with obesity.
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Relationship between polymorphism in the CRH gene and the traits of body conformation of Salers cows
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Bacterial cellulose as a support for yeast immobilization – Correlation between carrier properties and process efficiency
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Significant enhancement of citric acid production by Yarrowia lipolytica immobilized in bacterial cellulose-based carrier
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Flavonoid biosynthetic pathways in plants: Versatile targets for metabolic engineering
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The enhancement of anti-staphylococcal potential of plant derived naphthoquinones as a result of combination with silver nanoparticles
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Fingerprinting, structure, and genetic relationships among selected accessions of blue honeysuckle (Lonicera caerulea L.) from European collections
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Proteases immobilized on nanomaterials for biocatalytic, environmental and biomedical applications: Advantages and drawbacks
PublikacjaProteases have gained significant scientific and industrial interest due to their unique biocatalytic characteristics and broad-spectrum applications in different industries. The development of robust nanobiocatalytic systems by attaching proteases onto various nanostructured materials as fascinating and novel nanocarriers has demonstrated exceptional biocatalytic performance, substantial stability, and ease of recyclability over...
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Production of trehalose in a single step enzymatic reaction
PublikacjaThe aim of this study was isolation and cloning of trehalose synthase gene derived from extremophilic microorganisms Deinoccoci to the expressive E. coli vectors and its biosythesis in different hosts.
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Isolation and properties of recombinant trehalose synthase
PublikacjaBiosynthesis of trehalose is carried out via various matabolic pathways, including the use of trehalose synthase. Bacteria of the genus Deionococcus are microorganisms which produce trehalose synthase catalizing conversion of maltose into trehalose. In this work recombinant trehalose synthase from D.radiodurans was characterized.
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Interspecies distances between propionic acid degraders and methanogens in syntrophic consortia for optimal hydrogen transfer
PublikacjaA mixed culture from an anaerobic biowaste digester was enriched on propionate and used to investigate interspecies hydrogen transfer in dependence of spatial distances between propionate degraders and methanogens. From 20.3 mM propionate, 20.8 mM acetate and 15.5 mM methane were formed. Maximum specific propionate oxidation and methane formation rates were 49 and 23 mmol mg−1day−1, respectively. Propionate oxidation was inhibited by...
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A cryptic ribosome binding site, false signals in reporter systems and avoidance of protein translation chaos
PublikacjaThe expression of reporter gene may be induced by activation of cryptic signalling sequences, as we found while constructing the mutS-lacZ fusion gene. We cloned the Escherichia coli lacZ gene encoding beta-galactosidase into a plasmid vector carrying the Thermus thermophilus mutS gene. The clones expected to produce beta-galactosidase as the C-terminal fusion were selected for the complementation of beta-galactosidase activity...
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Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors
PublikacjaHairy root cultures of Hyoscyamus niger were cultivated in shake-flasks, a bubble-column bioreactor and a hybrid bubble-column/spray bioreactor and evaluated for alkaloid production.
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Study on a polish peat bog “Rucianka” as a source of yeast strains capable of effective phenol biodegradation
PublikacjaPhenol is one of the most widely distributed environmental pollutants which can be found in wastewaters and industrial effluents. Due to its toxicity and resistance to self-degradation, it could become even lethal for humans and animals. The treatment of this toxic compound is focused on psychical–chemical methods, although biological treatment of phenol is preferable for economic aspects related to relatively...
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Expression of a GDSL esterase from Pseudomonas sp. S9 in Pichiapastoris
PublikacjaCold active lipolytic enzymes are promising to replace the conventional enzymes processes of biotechnological industries. One of the most important feature of the cold-active lipases and esterases is that they offer economic benefits through energy saving. In general, they exhibit high activity at low temperatures and low thermostability at moderate temperatures. Lipolytic enzyme EstS9 was isolated from Pseudomonas sp. S9. A multiple...
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A Colorimetric Microplate Assay for DNA-Binding Activity of His-Tagged MutS Protein
PublikacjaA simple microplate method was designed for rapid testing DNA-binding activity of proteins. The principle of the assay involves binding of tested DNA by his-tagged protein immobilized on a nickel-coated ELISA plate, following colorimetric detection of biotinylated DNA with avidin conjugated to horseradish peroxidase. The method was used to compare DNA mismatch binding activities of MutS proteins from three bacterial species. The...
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Characterization of d-xylose reductase, XyrB, from Aspergillus niger
PublikacjaD-xylose reductase is a member of the aldo-keto reductase family, and is involved in D-xylose and L-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second D-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert D-xylose and L-arabinose, and it has the highest affinity for these sugars...
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cDNA fingerprint from the hepatopancreatic glands of pond snails (Lymnaea stagnalis) exposed to benzo[a]pyrene
PublikacjaIdentification of differentially expressed genes that could be potentially used as biomarkers of PAH exposure of common invertebrate animal (like freshwater snail) would be a valuable resource for investigators interested in toxicology and biomonitoring of aquatic environments. Therefore, the aim of this research was to investigate effects of waterborne benzo[a]pyrene (B[a]P) exposure on mRNA expression in the pond snail’s (Lymnaea...
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In vitro affinity of Deinococcus radiodurans MutS towards mismatched DNA exceeds that of its orthologues from Escherichia coli and Thermus thermophilus
PublikacjaThe mismatch binding protein MutS is responsible for the recognition of mispaired and unpaired bases, which is the initial step in DNA repair. Among the MutS proteins most extensively studied in vitro are those derived from Thermus thermophilus, Thermus aquaticus and Escherichia coli. Here, we present the first report on the in vitro examination of DNA mismatch binding activity of MutS protein from Deinococcus radiodurans and confront...
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The construction of bifunctional fusion proteins consisting of MutS and GFP
PublikacjaSkonstruowano białka chimeryczne zawierające białko MutS z Thermus thermophilus i białko zielonej fluoresceiny GFP z Aequorea victoria, posiadające dla łatwiejszego oczyszczania domeny oligo-histydynowe na N- lub C-końcu. Fuzyjne białka rozpoznawały niekomplementarności w DNA podobnie do MutS T. thermophilus. Fluorescencyjne białka rozpoznające niekomplementarne DNA mogą być użyteczne w wykrywaniu jednonukleotydowych polimorfizmów...
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Engendering responsibility in architecture students through real planning for a tram depot in Gdańsk
PublikacjaApart from technical competencies, the profession of architect requires specific social competencies: understanding the user’s needs, ability to diagnose social problems, negotiating and understanding the impact of construction investments on society and the environment. The architect plays a significant role in the collective effort to create spatial order. This is why it is important to include these social competencies in the...
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A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms
PublikacjaABSTRACT Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic...