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Wyniki wyszukiwania dla: beta-lactamase genes
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Identification and Analysis of Candidate Genes Associated with Yield Structure Traits and Maize Yield Using Next-Generation Sequencing Technology
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Thermostable beta-glucosidase with a broad substrate specifity suitable for processing of lactose-containing products.
PublikacjaHipertermofilny archeon Sulfolobus shibatae wytwarza termostabilną beta glukozydazę przydatną do hydrolizy laktozy w mleku i serwatce przejawiającą największą aktywność w 85 stC przy pH 5,5. Aktywność ekstraktu bezkomórkowego Sulfolobus shibatae wynosi 0.29 U/mg białka i może być 17-krotnie zwiększona poprzez frakcjonowanie siarczanem amonu, chromatografię jonowymienną i filtrację żelową. Badany enzym ma dużą termostabilność i...
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Thermostable Pyrococcus woesei beta-D-galactosidase - high level expression, purification and biochemical properties
PublikacjaGen kodujący termostabilną beta-D-galaktozydazę Pyrococcus woesei zamplifikowano z zastosowaniem techniki PCR i klonowano w komórkach Escherichia coli. Skonstruowany system ekspresji genu pozwolił na uzyskanie znacznej ilości termostabilnego białka w komórkach mezofilnego gospodarza. Enzym oczyszczono z zastosowaniem chromatografii powinowactwa po wcześniejszej denaturacji termicznej białek Escherichia coli i scharakteryzowano...
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MM/PBSA analysis of molecular dynamics simulations of bovine beta-lactoglobulin: free energy gradients in conformational transitions?
PublikacjaPraca dotyczy pH zależnych zmian konformacyjnych EF pętli beta-laktoglobuliny. Zmiany te są śledzone za pomocą metod obliczeniowych chemii (dynamika molekularna oraz metody MM/PBSA).
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Influence of Escherichia coli on Expression of Selected Human Drug Addiction Genes
PublikacjaThe impact of enteric microflora on the expression of genes associated with cocaine and amphetamine addiction was described. Human genome-wide experiments on RNA transcripts expressed in response to three selected Escherichia coli strains allowed for significant alteration (p > 0.05) of the linear regression model between HT-29 RNA transcripts associated with the KEGG pathway:hsa05030:Cocaine addiction after 3 h stimulation with...
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Improved magnitude estimation of complex numbers using alpha max and beta min algorithm
PublikacjaThe paper presents an improved algorithm for calculating the magnitude of complex numbers. This problem, which is a special case of square rooting, occurs for example, in FFT processors and complex FIR filters. The proposed method of magnitude calculation makes use of the modified alpha max and beta min algorithm. The improved version of the algorithm allows to control the maximum magnitude approximation error by using an adequate...
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Identification and cloning of C. albicans SC5314 genes encoding L-methionine biosynthetic pathway enzymes.
Dane BadawczeEnzymes of fungal L-methionine biosynthetic pathway: homoserine O-acetyltransferase (Met2p), O-acetylhomoserine sulfhydrylase (Met15p) and cystathionine-γ-synthase (Str2p) could be exploited as molecular targets for antifungal chemotherapy. The goal of the study was to identify and clone genes encoding mentioned above enzymes. MET2, MET15 and STR2 genes...
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Sensitive Demonstration of the Twin-Core Couplers including Kerr Law Non-Linearity via Beta Derivative Evolution
PublikacjaTo obtain new solitary wave solutions for non-linear directional couplers using optical meta-materials, a new extended direct algebraic technique (EDAT) is used. This model investigates solitary wave propagation inside a fiber. As a result, twin couplers are the subject of this study. Kerr law is the sort of non-linearity addressed there. Because it offers solutions to problems with large tails or infinite fluctuations, the resulting...
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DEVELOPMENT GENES AND EVOLUTION
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GENES & GENETIC SYSTEMS
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GENES BRAIN AND BEHAVIOR
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Implementation of magnitude calculation of complex numbers using improved alpha max plus beta min algorithm
PublikacjaThe paper presents the hardware implementation of the improved alpha max plus beta min algorithm for calculating the magnitude of complex numbers. This version of the algorithm requires the general division which is performed using a noniterative multiplicative division algorithm. We analyze in detail the division algorithm, its error and the impact of finite word-length signal representations on the assumed total computation error....
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Expression Profiling of the Slow Rusting Resistance Genes Lr34/Yr18 and Lr67/Yr46 in Common Wheat (Triticum aestivum L.) and Associated miRNAs Patterns
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Epigenetic regulation and regeneration: the search for differentially methylated genes in the MRL mouse
PublikacjaThe MRL mouse is an inbred laboratory strain, which was developed in the 60’s of the 20th century and has been extensively used as a model of lupus erythematosus. The regenerative abilities of the strain were discovered in the 90' when the MRL mouse was shown to close 2 mm hole punches made in the ear pinnae four weeks after injury without scarring. The phenomenon has not been observed in other mouse strains, where the holes...
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Isolation and Self-Association Studies of Beta-Lactoglobulin
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Beta-galactosidase activity of Meiothermus ruber cells
PublikacjaFreeze-dried cells of Meiothermus ruber catalyses cleavage of o-nitrophenyl-b-D-galactopiranoside (oNPb-gal) and conversion of lactose into glucose and galactose. The permeabilization with 2%toluene,20%ethanol and 20%acetone increased enzymatic activity from 74.87 U/g of lyophilized cells up to 129.44,114.38 and 90.19 U/g,respectively. Ethanol was an effective permeabilizing agent and its efficiency was dependent on the concentration,...
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Epidemiological Analysis of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Outbreak in a Neonatal Clinic in Poland
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Wykorzystanie komórek Escherichia coli transformowanych genem termostabilnej beta-galaktozydazy z Pyrococcus woesei do wytwarzania galaktozylofruktozy
PublikacjaKomórki rekombinantowej Escherichia coli transformowane genem beta-galaktozydazy z Pyrococcus woesei wykorzystano jako biokatalizator syntezy galaktozylofruktozy. Największą aktywność syntezy wymienionego prebiotyku (7,6 g/L x godz)uzyskano w 80C przy pH 5,4, stosując jako substrat roztwór zawierający 10% laktozy i 20% fruktozy. Aktywność transgalaktozylacji nie ulegała istotnym zmianom podczas sześciu kolejnych szarż produkcyjnych.
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Beta-blockers in the environment: Part II. Ecotoxicity study
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A bifunctional chimeric protein consisting of MutS and beta-galactosidase
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Intra-operative biopsy in chronic sinusitis detects pathogenic Escherichia coli that carry fimG/H, fyuA and agn43 genes coding biofilm formation
PublikacjaThe aim of this study was to investigate whether or not surgical biopsy of sinus tissue in chronic sinusitis, not responsive to treatment, would detect E. coli. We intended to evaluate E. coli virulence genes, therefore dispute the causal role of such an unusual microorganism in chronic sinusitis, as well as consider effective pathogen-targeted therapy. Patients with E. coli isolated by intra-operative puncture biopsy were included...
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Immobilized preparation of cold-adapted and halotolerant Antarctic β-galactosidase as a highly stable catalyst in lactose hydrolysis
PublikacjaZimnolubna rekombinowana beta-galaktozydaza antarktycznej morskiej bakterii Pseudoalteromonas sp. 22b została immobilizowana na chitozanie za pomocą aldehydu glutarowego. Preparat immobilizowanego enzymu w przeciwieństwie do preparatu enzymu w buforze reakcyjnym nie był inhibowany przez glukozę. Immobilizacja enzymu wpłynęła na podwyższenie o 10°C optymalnej temperatury aktywności względem jego nieimmobilizowanego odpowiednika...
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Predicting Value of Binding Constants of Organic Ligands to Beta-Cyclodextrin: Application of MARSplines and Descriptors Encoded in SMILES String
PublikacjaThe quantitative structure–activity relationship (QSPR) model was formulated to quantify values of the binding constant (lnK) of a series of ligands to beta–cyclodextrin (β-CD). For this purpose, the multivariate adaptive regression splines (MARSplines) methodology was adopted with molecular descriptors derived from the simplified molecular input line entry specification (SMILES) strings. This approach allows discovery of regression...
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G3-Genes Genomes Genetics
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Relationship between the betalain composition in Opuntia ficus indica and Beta vulgaris varieties and biological activity of their extracts
PublikacjaBetalains are a class of pigments that are much less com- monly found in nature than anthocyanins or carotenoids. They occur only in a few edible plants such as red beetroot (Beta vulgaris), prickly pears (Opuntia ficus indica), amaranth (Amaranthus sp.) and some mushrooms. This group of pig- ments can be divided into two major groups: yellow vulgax- anthins and red betanins. Despite their structural similarity to compounds from...
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A subset of two adherence systems, acute pro-inflammatory pap genes and invasion coding dra, fim, or sfa, increases the risk of Escherichia coli translocation to the bloodstream
PublikacjaAn analysis of the phylogenetic distribution and virulence genes of Escherichia coli isolates which predispose this bacteria to translocate from the urinary tract to the bloodstream is presented. One-dimensional analysis indicated that the occurrence of P fimbriae and α-hemolysin coding genes is more frequent among the E. coli which cause bacteremia. However, a two-dimensional analysis revealed that a combination of genes coding...
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Seasonality of Psoriasis: From Genes to the Clinical Phenomena
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System pomiaru szybkości korozji metodą polaryzacji liniowej w zbiorniku zatłaczającym wodę złożową oraz systemu wody morskiej na platformie wydobywczej Baltic Beta
PublikacjaWykonano analizę sprawności systemu monitorowania szybkości korozji na Platformie wydobywczej Baltic Beta. Dokonano niezbędnych czynności, które umożliwiły ponowne uruchomienie systemu. Przedstawiono rejestry szybkości korozji w okresie od 01.01.2015 do 24.11.2016
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Beta-blockers in the environment: Part I. Mobility and hydrolysis study
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Beta-2 adrenoreceptor gene polymorphisms and sympathetic outflow in humans
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Beta-galactosidases from a psychrotolerant Arthrobacter isolates and their potential use
PublikacjaEnzymes from extremophiles, especially psychrophiles and psychrotolerant microorganisms are currently intensively studied because of their unusual structural and functional properties. One of these enzymes is a cold-adapted - galactosidase (EC 3.2.1.23) which catalyzes the hydrolysis of - 1,4-glycosidic bonds in -galactosides. The most common - galactoside is lactose, a disaccharide which makes up 2-7% of milk. The majority of...
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VIRUS GENES
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Complete Nucleotide Sequence of the pCTX-M3 Plasmid and Its Involvement in Spread of the Extended-Spectrum β-Lactamase GeneblaCTX-M-3
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Active Site Architecture and Reaction Mechanism Determination of Cold Adapted beta-D-galactosidase from Arthrobacter sp. 32cB
PublikacjaArthbetaDG is a dimeric, cold-adapted beta-D-galactosidase that exhibits high hydrolytic and transglycosylation activity. A series of crystal structures of its wild form, as well as its ArthbetaDG_E441Q mutein complexes with ligands were obtained in order to describe the mode of its action. The ArthbetaDG_E441Q mutein is an inactive form of the enzyme designed to enable observation of enzyme interaction with its substrate. The...
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The Combination of Beta-Blockers and ACE Inhibitors Across the Spectrum of Cardiovascular Diseases
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Synthesis and Study of Antifungal Properties of New Cationic Beta-Glucan Derivatives
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Biofilm Production and Presence of ica and bap Genes in Staphylococcus aureus Strains Isolated from Cows with Mastitis in the Eastern Poland
PublikacjaThe aim of the study was phenotypic and genotypic analysis of 132 S. aureus strains isolated from mastitis in eastern Poland in respect to their biofilm formation ability. The analysis of the size polymorphism of fragment X in the gene encoding protein A (spa) revealed high genetic differentiation of the analyzed group of isolates. The ability of biofilm formation by the isolates was tested using two phenotypic methods. The Congo...
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Common variants of DNA repair genes and malignant melanoma
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The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)
PublikacjaThe aim of this study was to analyze the occurrence of sulfonamide resistance genes (sul1–3) and other genetic elements as antiseptic resistance gene (qacEΔ1) and class 1 and class 2 integrons (int1–2) in the upper layer of substrate and in the effluent of microcosm constructed wetlands (CWs) treating artificial wastewater containing diclofenac and sulfamethoxazole (SMX), which is a sulfonamide antibiotic. The bacteria in the substrate...
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A method for the production of D-tagatose using a recombinant Pichia pastoris strain secreting beta-D-galactosidase from Arthrobacter chlorophenolicus and a recombinant L-arabinose isomerase from Arthrobacter sp. 22c
PublikacjaD-Tagatose is a natural monosaccharide which can be used as a low-calorie sugar substitute in food, beverages and pharmaceutical products. It is also currently being tested as an anti-diabetic and obesity control drug. D-Tagatose is a rare sugar, but it can be manufactured by the chemical or enzymatic isomerization of D-galactose obtained by a beta-D-galactosidase-catalyzed hydrolysis of milk sugar lactose and the separation of...
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Mosaic Structure of p1658/97, a 125-Kilobase Plasmid Harboring an Active Amplicon with the Extended-Spectrum β-Lactamase Gene bla SHV-5
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A convenient synthesis of (S)-O-benzyl-N-tert-butoxycarbonyl-beta-tyrosine.
PublikacjaOpracowano dogodną metodę syntezy tytułowego aminokwasu z aldehydu 4-benzyloksybenzoesowego, kwasu malonowego i octanu amonu. Otrzymany aminokwasu rozdzielono na enancjomery poprzez krystalizację soli diastereoizomerycznej z kwasem L-winowym.
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Anticancer properties of low molecular weight oat beta-glucan – An in vitro study
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System monitorowania korozji w instalacjach wodnych platformy wydobywczej Baltic Beta
PublikacjaRealizacja doktoratu rozwiązała problemy korozyjne w jednym ze strategicznych obszarów procesu eksploatacji ropy. Polegała na uruchomieniu monitoringu korozji w instalacji zatłaczającej wodę morską do złoża ropy, na platformie wydobywczej firmy LOTOS Petrobaltic. Wdrożono automatyczny system monitorowania korozji metodą polaryzacji liniowej, oszacowano korozyjność uzdatnionej wody, zidentyfikowano rodzaje korozji występujące w...
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Beware of the crash risk: Tail beta and the cross-section of stock returns in China
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Common variants of xeroderma pigmentosum genes and prostate cancer risk
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Estrogen receptors genes polymorphisms and age at menarche in idiopathic scoliosis
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Association of Genes Related to Oxidative Stress with the Extent of Coronary Atherosclerosis
PublikacjaOxidative stress is believed to play a critical role in atherosclerosis initiation and progression. In line with this, in a group of 1099 subjects, we determined eight single nucleotide polymorphisms (SNPs) related to oxidative stress (PON1 c.575A>G, MPO c.463G>A, SOD2 c.47T>C, GCLM c.590C>T, NOS3 c.894G>T, NOS3 c.786T>C, CYBA c.214C>T, and CYBA c.932A>G) and assessed the extent of atherosclerosis in coronary arteries based on...
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Antibacterial and Anti-Biofilm Activities of Essential Oil Compounds against New Delhi Metallo-β-Lactamase-1-Producing Uropathogenic Klebsiella pneumoniae Strains
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Effects of water level and grassland management on alpha and beta diversity of birds in restored wetlands
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