Wyniki wyszukiwania dla: beta-lactamase genes
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Neural oscillations induced by IAPS pictures (session 1)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 9)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 5)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 2)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 11)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 3)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 6)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 4)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 8)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 7)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 14)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 13)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 16)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 19)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 17)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 22)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 15)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 21)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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Neural oscillations induced by IAPS pictures (session 20)
Dane BadawczeThe data were collected to perform research on the possibility of controlling the content displayed on the monitor screen using human emotional states extracted from EEG signals. The dataset contains recordings of 14-channel EEG signals gathered from seven persons aged 23 - 35 within 26 sessions, during which 45 different random photos, taken from the...
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IS THE REGIONAL DIVERGENCE A PRICE FOR THE INTERNATIONAL CONVERGENCE? THE CASE OF THE VISEGRAD GROUP
PublikacjaThe main goal of this article is to verify whether the Visegrad Group countries are achieving social convergence at national level at the expense of internal divergence. For this purpose, the existence of social beta-, sigma- and gamma-convergence at national and regional level was tested. The author of this article decided to tackle the convergence problem since there is a high risk that countries are trying to catch up at...
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The SEM images of Ce0.8La0.2O2 nanocatalyst infiltrated with 10wt.% Ni prepared using soft template method
Dane BadawczeThe dataset includes SEM images of Ce0.8La0.2O2-s nanopowder (nanoparticles) prepared using soft template method and infiltrated to 10wt.% Ni in the catalyst. CTAB was used as a micelle-based templating agent. The powder was precipitated from aqueous nitrate solution using NaOH solution and 250xCMC (critcal micelle concentration) of CTAB. Precipitate...
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Nitric Oxide-Dependent Pathways as Critical Factors in the Consequences and Recovery after Brain Ischemic Hypoxia
PublikacjaBrain ischemia is one of the leading causes of disability and mortality worldwide. Nitric oxide (NO), a molecule that is involved in the regulation of proper blood flow, vasodilation, neuronal and glial activity constitutes the crucial factor that contributes to the development of pathological changes after stroke. One of the early consequences of a sudden interruption in the cerebral blood flow is the massive production of reactive...
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An MTA-phosphorylase gene discovered in the metagenomic library derived from Antarctic top soil during screening for lipolytic active clones confers strong pink fluorescence in the presence of rhodamine B.
PublikacjaIn this work, we present the construction of a metagenomic library in Escherichiacoli using the pUC19 vector and environmental DNA directly isolated fromAntarctic topsoil and screened for lipolytic enzymes. Unexpectedly, the screeningon agar supplemented with olive oil and rhodamine B revealed one unusual pinkfluorescent clone (PINKuv) out of 85 000 clones. This clone harbored a plasmid,pPINKuv, which has an insert of 8317 bp that...
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Examination of cyp51A and cyp51B expression level of the first Polish azole resistant clinical Aspergillus fumigatus isolate
PublikacjaAspergillus fumigatus is one of the most prevalent airborne fungal pathogens causing infections worldwide. Most A. fumigatus strains are susceptible to azoles, which are administered as the first line therapeutics. However, during last decade the acquired resistance to triazoles by these species has been described. There is a number of publications concerning the examination of clinical A. fumigatus strains from different countries,...
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Modulation of CYP1A1, CYP1A2 and CYP1B1 expression by cabbage juices and indoles in human breast cell lines
PublikacjaEpidemiological studies have shown that consumption of cab- bage and sauerkraut is connected with significant reduction of breast cancer incidences. Estrogens are considered a major breast cancer risk factor and their metabolism by P450 enzymes substan- tially contributes to carcinogenic activity. The aim of this study was to investigate the effect of cabbage and sauerkraut juices of differ- ent origin on the expression profile...
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Independent dynamics of low, intermediate, and high frequency spectral intracranial EEG activities during human memory formation
PublikacjaA wide spectrum of brain rhythms are engaged throughout the human cortex in cognitive functions. How the rhythms of various frequency ranges are coordinated across the space of the human cortex and time of memory processing is inconclusive. They can either be coordinated together across the frequency spectrum at the same cortical site and time or induced independently in particular bands. We used a large dataset of human intracranial...
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Network oscillations modulate interictal epileptiform spike rate during human memory
PublikacjaEleven patients being evaluated with intracranial electroencephalography for medically resistant temporal lobe epilepsy participated in a visual recognition memory task. Interictal epileptiform spikes were manually marked and their rate of occurrence compared between baseline and three 2 s periods spanning a 6 s viewing period. During successful, but not unsuccessful, encoding of the images there was a significant reduction in...
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Estimating the parameter of inequality aversion on the basis of a parametric distribution of incomes
PublikacjaResearch background: In applied welfare economics, the constant relative inequality aversion function is routinely used as the model of a social decisionmaker’s or a society’s preferences over income distributions. This function is entirely determined by the parameter, ε, of inequality aversion. However, there is no authoritative answer to the question of what the range of ε an analyst should select for empirical work. Purpose...
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The influence of frequency separation on imaging properties in DFEIT
PublikacjaW artykule przedstawiono wpływ wyboru składowych częstotliwościowych dla różnicowej tomografii impedancyjnej na wynik i własności obrazowania w dwuczęstotliwościowej różnicowej tomografii impedancyjnej.A Dual Frequency EIT is an extension of a traditional EIT that uses two sinusoidal signals for imaging. Appropriate selection of signals' frequency allows to achieve reasonable contrast of imaged structure. It has already been shown...
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Analysis of the impact of wastewater discharge on recipients: synergistic approach
PublikacjaThe dissertation presents the analysis of chemical and microbial composition of wastewater and examines the impact of wastewater discharge on the environment. Various case studies were analysed: from small settlements in a pristine European Arctic, to large municipal wastewater treatment plants in areas subjected to greater anthropogenic pressure. In order to comprehensively analyse the impact of wastewater discharge on the receiving...
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Surgical Site Infection after Breast Surgery: A Retrospective Analysis of 5-Year Postoperative Data from a Single Center in Poland
PublikacjaBackground and Objectives: Surgical site infection (SSI) is a significant complication of non-reconstructive and reconstructive breast surgery. This study aimed to assess SSI after breast surgery over five years in a single center in Poland. The microorganisms responsible for SSI and their antibiotic susceptibilities were determined. Materials and Methods: Data from 2129 patients acquired over five years postoperatively by the...
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The XRD diffraction patterns of Ce0.8La0.2O2/Ni prepared using soft template method and impregnation
Dane BadawczeThe dataset includes XRD patterns of Ce0.8La0.2O2-s nanopowders (nanoparticles,nanocatalyst) prepared using soft template method and further impregnated with 10wt.%Ni using aqueous nitrate solution.
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c-Myc Protein Level Affected by Unsymmetrical Bisacridines Influences Apoptosis and Senescence Induced in HCT116 Colorectal and H460 Lung Cancer Cells
PublikacjaUnsymmetrical bisacridines (UAs) are highly active antitumor compounds. They contain in their structure the drugs previously synthesized in our Department: C-1311 and C-1748. UAs exhibit different properties than their monomer components. They do not intercalate to dsDNA but stabilize the G-quadruplex structures, particularly those of the MYC and KRAS genes. Since MYC and KRAS are often mutated and constitutively expressed in cancer...
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May Staphylococcus lugdunensis Be an Etiological Factor of Chronic Maxillary Sinuses Infection?
PublikacjaStaphylococcus lugdunensis is an opportunistic pathogen found in the healthy human skin microbiome bacterial community that is able to cause infections of diverse localization, manifestation, and course, including laryngological infections, such as necrotizing sinusitis. Chronic maxillary sinusitis is a disease present in up to one third of European and American populations, and its etiology is not fully described. Within this...
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Antibiotic resistance in wastewater, does the context matter? Poland and Portugal as a case study
PublikacjaAntibiotic resistance has been considered a major human health threat that may endanger the success of medicine. Recent studies have unveiled worldwide asymmetries of antibiotic resistance occurrence, being factors as diverse as climate, socioeconomic, or antibiotic use possible drivers of such asymmetric distribution. In Europe, where clinical antibiotic resistance is surveyed for more than 20 years, the European Center for Disease...
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Impact of soluble organic matter and particulate organic matter on anammox system: Performance, microbial community and N2O production
PublikacjaIn this study, the effects of soluble readily biodegradable COD (sCOD) and particulate slowly biodegradable COD (pCOD) on anammox process were investigated. The results of the longterm experiment indicated that a low sCOD/N ratio of 0.5 could accelerate the anammox and denitrification activity, to reach as high as 84.9% ±2.8% TN removal efficiency. Partial denitrification-anammox (PDN/anammox) and denitrification were proposed...
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Interactions between polyphenolic antioxidants quercetin and naringenin dictate the distinctive redox-related chemical and biological behaviour of their mixtures
PublikacjaFood synergy concept is suggested to explain observations that isolated antioxidants are less bioactive than real foods containing them. However, mechanisms behind this discrepancy were hardly studied. Here, we demonstrate the profound impact of interactions between two common food flavonoids (individual: aglycones quercetin—Q and naringenin—N− or their glycosides rutin—R and naringin—N+ vs. mixed: QN− and RN+) on their electrochemical...
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Anticancer Imidazoacridinone C-1311 is Effective in Androgen-Dependent and Androgen-Independent Prostate Cancer Cells
PublikacjaAndrogen receptor (AR) plays a crutial role in prostate cancer (PCa) development and metastasis. Here, we reported potent anti-PCa activity of a small molecule imidazoacridinone C-1311. In AR-positive PCa cells, C-1311 was found to inhibit the transcriptional activity of AR uncovering a novel mechanism that may be relevant for its anticancer effect. Mechanistically, C-1311 decreased AR binding to prostate-specific antigen (PSA)...
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Regulation of the First Committed Step in Lipopolysaccharide Biosynthesis Catalyzed by LpxC Requires the Essential Protein LapC (YejM) and HslVU Protease
PublikacjaWe previously showed that lipopolysaccharide (LPS) assembly requires the essential LapB protein to regulate FtsH-mediated proteolysis of LpxC protein that catalyzes the first committed step in the LPS synthesis. To further understand the essential function of LapB and its role in LpxC turnover, multicopy suppressors of ΔlapB revealed that overproduction of HslV protease subunit prevents its lethality by proteolytic degradation...
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Regulated Assembly of LPS, Its Structural Alterations and Cellular Response to LPS Defects
PublikacjaDistinguishing feature of the outer membrane (OM) of Gram-negative bacteria is its asymmetry due to the presence of lipopolysaccharide (LPS) in the outer leaflet of the OM and phospholipids in the inner leaflet. Recent studies have revealed the existence of regulatory controls that ensure a balanced biosynthesis of LPS and phospholipids, both of which are essential for bacterial viability. LPS provides the essential permeability...
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Two highly thermostable paralogous single-stranded DNA-binding proteins from Thermoanaerobacter tengcongensis
PublikacjaThe thermophilic bacterium Thermoanaerobacter tengcongensis has two single-stranded DNA-binding (SSB) proteins, designated TteSSB2 and TteSSB3. In a SSB complementation assay in Escherichia coli, only TteSSB3 took over the in vivo function of EcoSSB. We have cloned the ssb genes obtained by PCR and have developed E. coli overexpression systems. The TteSSB2 and TteSSB3 consist of 153 and 150 amino acids with a calculated molecular...
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Identification and properties of the psychrophilic bacterium Desulfolatea psychrophila single-stranded DNA binding proteins
PublikacjaTo study the biochemical properties of SSB from Desulfolatea psychrophila (DpsSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 426 nucleotides encoding SSB protein of 142 amino acids with a calculated molecular mass of 15.6 kDa and it is the smallest known bacterial SSB protein indentified to date. The amino-acid sequence...
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Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland)
PublikacjaIn this study, antimicrobial-resistance patterns were analyzed in Escherichia coli isolates from raw (RW) and treated wastewater (TW) of two wastewater treatment plants (WWTPs), their marine outfalls (MOut), and mouth of the Vistula River (VR). Susceptibility of E. coli was tested against different classes of antibiotics. Isolates resistant to at least one antimicrobial agent were PCR tested for the presence of integrons. Ampicillin-resistant...
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A new B-D-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization.
PublikacjaA psychrotrophic bacterium producing a coldadaptedB-galactosidase upon growth at low temperatureswas classiWed as Arthrobacter sp. 20B. A genomic DNAlibrary of strain 20B introduced into Escherichia coliTOP10F' and screening on X-Gal (5-bromo-4-chloro-3-indolyl-B-D-galactopyranoside)-containing agar plates ledto the isolation of B-galactosidase gene. The B-galactosidasegene (bgaS) encoding a protein of 1,053 amino acids,with a...
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Mapping the Transglycosylation Relevant Sites of Cold-Adapted β-D-Galactosidase from Arthrobacter sp. 32cB
PublikacjaB-Galactosidase from Arthrobacter sp. 32cB (ArthbetaDG) is a cold-adapted enzyme able to catalyze hydrolysis of beta-D-galactosides and transglycosylation reaction, where galactosyl moiety is being transferred onto an acceptor larger than a water molecule. Mutants of ArthbetaDG D207A and E517Q were designed to determine the significance of specific residues and to enable formation of complexes with lactulose and sucrose and to...
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Checkpoints that regulate balanced biosynthesis of lipopolysaccharide and its essentiality in Escherichia coli
PublikacjaThe outer membrane (OM) of Gram-negative bacteria, such as Escherichia coli, is essential for their viability. Lipopolysaccharide (LPS) constitutes the major component of OM, providing the permeability barrier, and a tight balance exists between LPS and phospholipids amounts as both of these essential components use a common metabolic precursor. Hence, checkpoints are in place, right from the regulation of the first committed step...
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imPlatelet classifier: image‐converted RNA biomarker profiles enable blood‐based cancer diagnostics
PublikacjaLiquid biopsies offer a minimally invasive sample collection, outperforming traditional biopsies employed for cancer evaluation. The widely used material is blood, which is the source of tumor-educated platelets. Here, we developed the imPlatelet classifier, which converts RNA-sequenced platelet data into images in which each pixel corresponds to the expression level of a certain gene. Biological knowledge from the Kyoto Encyclopedia...
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Differential expression profile between amygdala and blood during chronic lithium treatment in a rat model of depression – a pilot study
PublikacjaLithium is a mood stabilizer widely used in the pharmacotherapy of bipolar disorder and treatment‑resistant depression. Taking into account dysregulated inflammatory activity in depression and the immunomodulatory role of lithium, we hypothesized that genes associated with inflammatory responses may be potential biomarkers of lithium action. We aimed to compare gene expression changes between the brain and the periphery after...
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Platelet RNA Sequencing Data Through the Lens of Machine Learning
PublikacjaLiquid biopsies offer minimally invasive diagnosis and monitoring of cancer disease. This biosource is often analyzed using sequencing, which generates highly complex data that can be used using machine learning tools. Nevertheless, validating the clinical applications of such methods is challenging. It requires: (a) using data from many patients; (b) verifying potential bias concerning sample collection; and (c) adding interpretability...
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A vector-enzymatic DNA fragment amplification-expression technology for construction of artificial, concatemeric DNA, RNA and proteins for novel biomaterials, biomedical and industrial applications
PublikacjaA DNA fragment amplification/expression technology for the production of new generation biomaterials for scientific, industrial and biomedical applications is described. The technology enables the formation of artificial Open Reading Frames (ORFs) encoding concatemeric RNAs and proteins. It recruits the Type IIS SapI restriction endonuclease (REase) for an assembling of DNA fragments in an ordered head-to-tail-orientation. The...