Wyniki wyszukiwania dla: dna purine bases

• Video Classification Technology in a Knowledge-Vision-Integration Platform for Personal Protective Equipment Detection: An Evaluation

  Publikacja

  • C. De
  • C. Sanin
  • E. Szczerbicki

  - Rok 2018

  This work is part of an effort for the development of a Knowledge-Vision Integration Platform for Hazard Control (KVIP-HC) in industrial workplaces, adaptable to a wide range of industrial environments. This paper focuses on hazards resulted from the non-use of personal protective equipment (PPE), and examines a few supervised learning techniques to compose the proposed system for the purpose of recognition of three protective...

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• A universal method for the identification of genes encoding amatoxins and phallotoxins in poisonous mushrooms

  Publikacja

  • A. Wołoszyn
  • R. Kotlowski

  - Roczniki Państwowego Zakładu Higieny - Rok 2017

  ABSTRACT Background. As the currently known diagnostic DNA targets amplified in the PCR assays for detection of poisonous mushrooms have their counterparts in edible species, there is a need to design PCR primers specific to the genes encoding amanitins and phallotoxins, which occur only in poisonous mushrooms. Objective. The aim of the study was testing of PCR-based method for detection of all genes encoding hepatotoxic cyclic...

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• Dissociative Electron Attachment to 5-Iodo-4-thio-2′-deoxyuridine: A Potential Radiosensitizer of Hypoxic Cells

  Publikacja

  • M. Saqib
  • E. Arthur-Baidoo
  • F. Izadi
  • A. Szczyrba
  • M. Datta
  • S. Demkowicz
  • J. Rak
  • S. Denifl

  - Journal of Physical Chemistry Letters - Rok 2023

  In the search for effective radiosensitizers for tumor cells, halogenated uracils have attracted more attention due to their large cross section for dissociation upon the attachment of low-energy electrons. In this study, we investigated dissociative electron attachment (DEA) to 5-iodo-4-thio-2'-deoxyuridine, a potential radiosensitizer using a crossed electron-molecule beam experiment coupled with quadrupole mass spectrometry....

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• Zespół Systemów Geoinformatycznych

  Zespoły Badawcze

  • Katedra Systemów Geoinformatycznych

  W katedrze prowadzone są badania naukowe w zakresie szeroko rozumianych Systemów Informacji Geograficznej (GIS). Tematyka badań obejmuje zastosowanie GIS w technologiach bezpieczeństwa, wizualizacje i analizy przestrzenne, systemy numerycznego prognozowania pogody, technologie nawigacji w ramach mobilnych systemów informacji przestrzennej, oraz zaawansowane techniki obrazowania satelitarnego. Katedra kontynuuje również badania...

• Katedra Zarządzania

  Zespoły Badawcze

  • Katedra Zarządzania

  * zarządzanie wiedzą i informacją * zarządzanie strategiczne w wyższych uczelniach * wykorzystywanie metod nieparametrycznych do pomiaru efektywności systemów szkolnictwa wyższego * modele biznesowe w zarządzaniu organizacjami * zarządzanie procesem innowacyjnym w MŚP * strategia i modele biznesu współczesnego przedsiębiorstwa * społeczeństwo informacyjne i jego wskaźniki rozwoju * zarządzanie morskimi portami jachtowymi 2 gospodarce...

• Urządzenie samobieżne do badania środowiska wodnego, zwłaszcza do badania dna morskiego

  Wynalazki

  • Wydział Oceanotechniki i Okrętownictwa

• A novel cold-active beta-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization

  Publikacja

  • A. Wierzbicka-Woś
  • H. Cieśliński
  • M. Wanarska
  • K. Kozłowska-Tylingo
  • P. Hildebrandt
  • J. Kur

  - Microbial Cell Factories - Rok 2011

  Beta-D-galactosidase (EC 3.2.1.23) catalyze the hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Cold-active beta-D-galactosidases have recently become a focus of attention of researchers and dairy product manufactures owing to theirs ability to: (I) eliminate of lactose from refrigerated milk for people afflicted with lactose intolerance, (II) convert lactose to glucose and galactose which...

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• Context-Aware Indexing and Retrieval for Cognitive Systems Using SOEKS and DDNA

  Publikacja

  • C. De Silva Oliveira
  • C. Sanin
  • E. Szczerbicki

  - Advances in Intelligent Systems and Computing - Rok 2019

  Visual content searching, browsing and retrieval tools have been a focus area of interest as they are required by systems from many different domains. Context-based, Content-Based, and Semantic-based are different approaches utilized for indexing/retrieving, but have their drawbacks when applied to systems that aim to mimic the human capabilities. Such systems, also known as Cognitive Systems, are still limited in terms of processing...

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• Synthesis of eosin modified TiO2 film with co-exposed {001} and {101} facets for photocatalytic degradation of para-aminobenzoic acid and solar H2 production

  Publikacja

  • J. Khan
  • M. Sayed
  • N. S. Shah
  • S. Khan
  • Y. Zhang
  • G. Boczkaj
  • H. Khan
  • D. D. Dionysiou

  - APPLIED CATALYSIS B-ENVIRONMENTAL - Rok 2020

  Owing to the increasing photosensitivity and DNA damage properties of para-aminobenzoic acid (PABA), concerns have been raised over the exposure of humans to PABA. Solar light-driven photocatalysis (SPC) provides a promising solution for the effective removal of organic pollutants especially when directed towards sulfate radical (SO4−) production. Herein, we have developed a sulfite-enhanced SPC using Eosin-Y sensitized TiO2/Ti...

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• Solid Phase Synthesis and Biological Activity of Tuftsin Conjugates

  Publikacja

  • M. Kukowska-Kaszuba
  • K. Dzierzbicka
  • M. Serocki
  • A. Składanowski

  - JOURNAL OF MEDICINAL CHEMISTRY - Rok 2011

  New tuftsin/retro-tuftsin conjugates were designed and synthesized using a classical fluorenylmethoxycarbonyl (Fmoc) solid phase procedure. All the peptide conjugates were divided into three series: 1,4-dihydroxyanthraquinone (type A), 1-nitroacridine (type B), and 4-carboxyacridone (type C) derivatives. In type A conjugates, the N-terminal group of the peptide chain is directly connected to the anthraquinone ring at C1 (Scheme...

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• Five-hour diagnosis of dermatophyte nail infections with specific detection of Trichophyton rubrum

  Publikacja

  • A. Brillowska-Dąbrowska
  • D. M. Saunte
  • M. C. Arendrup

  - JOURNAL OF CLINICAL MICROBIOLOGY - Rok 2007

  W publikacji przedstawiono szybką dwuetapową metodę oczyszczania DNA oraz opartą na technice multiplex PCR metodę wykrywania grzybów z rodzaju dermatofitów oraz gatunku Trichophyton rubrum z hodowli grzybów oraz bezpośrednio ze zmienionych chorobowo paznokci. Testy stu osiemnastu próbek z użyciem zaprojektowanej reakcji multiplex PCR i dwóch kolejnych (jedna wykrywająca grzyby z rodzaju dermatofitów, druga wykrywająca gatunek T....

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• Zróżnicowanie bakterioplanktonu w wodach fiordów Spitsbergenu

  Publikacja

  • A. Kalinowska

  - Rok 2015

  Podczas rejsu polarnego latem 2013 roku w ramach projektów naukowych GAME i FACE2FACE zebrano materiał badawczy w dwóch fiordach Spitsbergenu: południowym Hornsund i północnym Kongsfjord. Punkty poboru próbek zlokalizowane były wzdłuż osi fiordów oraz w trójkącie o boku około 1,5 km wyznaczonym przez stacje w głębi fiordów. W zależności od punktu pobrano wodę z od 6 do 10 głębokości: od powierzchni do dna fiordu. Za pomocą fluorescencyjnego...

• Motility activity, slime production, biofilm formation and genetic typing by ERIC-PCR for Pseudomonas aeruginosa strains isolated from bovine and other sources (human and environment)

  Publikacja

  • K. Wolska
  • P. Szweda
  • K. Lada
  • E. Rytel
  • K. M. Gucwa
  • B. Kot
  • M. Piechota

  - POLISH JOURNAL OF VETERINARY SCIENCES - Rok 2014

  The molecular-typing strategy, ERIC-PCR was used in an attempt to determine the genomic relationship of 28 P. aeruginosa strains isolated from faeces of healthy bovine, bovine mastitis and from faeces of hospital patients as well as from environment. ERIC-PCR fingerprinting revealed large molecular differentiation within this group of isolates. Twenty two out of 28 strains tested generated unique patterns of DNA bands and only...

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• Experience-Oriented Intelligence for Internet of Things

  Publikacja

  • H. Zhang
  • F. Li
  • J. Wang
  • C. Sanín
  • E. Szczerbicki

  - CYBERNETICS AND SYSTEMS - Rok 2017

  The Internet of Things (IoT) has gained significant attention from industry as well as academia during the past decade. The main reason behind this interest is the capabilities of the IoT for seamlessly integrating classical networks and networked objects, and hence allows people to create an intelligent environment based on this powerful integration. However, how to extract useful information from data produced by IoT and facilitate...

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• Hazard Control in Industrial Environments: A Knowledge-Vision-Based Approach

  Publikacja

  • C. De
  • C. Sanin
  • E. Szczerbicki

  - Advances in Intelligent Systems and Computing - Rok 2018

  This paper proposes the integration of image processing techniques (such as image segmentation, feature extraction and selection) and a knowledge representation approach in a framework for the development of an automatic system able to identify, in real time, unsafe activities in industrial environments. In this framework, the visual information (feature extraction) acquired from video-camera images and other context based gathered...

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• EXPERIENCE-ORIENTED SMART EMBEDDED SYSTEM

  Publikacja

  • E. Szczerbicki
  • C. Sanin

  - Rok 2013

  The Experience-Oriented Smart Embedded System (EOSES) is proposed as a new technological platform providing a common knowledge management approach that allows mass embedded systems for experiential knowledge capturing, storage, involving, and sharing. Knowledge in the EOSES is represented as SOEKS, and organized as Decisional DNA. The platform is mainly based on conceptual principles from Embedded Systems and Knowledge Management....

• ARTIFICIAL MODEL IN THE ASSESSMENT OF THE ALGORITHM OF OBJECTS RECORDED BY LASER SCANNING SHAPE DETECTION (ALS/TLS)

  Publikacja

  • A. Janowski
  • P. Nierebiński
  • J. Szulwic

  - Rok 2015

  Brief description of the study and used methods. Brief description of the study and used As part of the preparatory work aimed to create the application solution allowing for the automation of searching objects in data, obtained in the scanning process using ALS (Airborne Laser Scanning) or TLS (Terrestrial Laser Scanning), the authors prepared a artificial (synthetic, theoretical) model of space, used for the verification of operation...

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• Urban Mentoring as a new polish management technique in participatory planning

  Publikacja

  • J. Martyniuk-Pęczek
  • G. Rembarz

  - Rok 2017

  A decade of experience in implementation of numerous urban modernization projects in Poland resulted in a lively debate on socialization of the planning process, in which the matter of the public space quality has become the center of gravity. In those years, transformation from the classic administrative model of „bureaucracy planning” to the model of „management planning” has not been complete. The socialist primacy of the public...

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• StryrKom StyrKom „Ocena wpływu polistyrenu pochodzącego z opakowań i materiałów termoizolacyjnych na DNA organizmów żywych z wykorzystaniem testu kometowego”- Radium

  Projekty

  Kierownik projektu: dr inż. Paweł Filipkowski

  Projekt realizowany w Katedra Chemii,Technologii i Biotechnologii Żywności zgodnie z porozumieniem DEC-2/RADIUM/2021 z dnia 2021-07-13

• Algorytmy analizy i przetwarzania danych z sonarów wielowiązkowych w rozproszonych systemach GIS

  Publikacja

  • A. Chybicki

  - Rok 2011

  Telemonitoring morski oraz szeroko rozumiane badania morza są ważnym elementem aktywności człowieka w sferze badań, nauki oraz gospodarki. Prowadzenie działań związanych z tworzeniem map dna, inspekcją nadbrzeży, umocnień, badaniem fauny morskiej pozwala zrozumieć procesy zachodzące w środowisku morskim oraz przyczynia się do rozwoju wielu gałęzi gospodarki takich jak transport morski, bezpieczeństwo, ochrona portów i inne. W ramach...

• Genetycznie modyfikowane mikroorganizmy narzędziem służącym do usuwania metali ciężkich ze środowiska

  Publikacja

  • M. Banasik

  - Rok 2012

  Metale ciężkie są pierwiastkami metalicznymi i półmetalicznymi o gęstości powyżej 4,5 g•cm-3. Powodują zaburzenia w strukturze DNA, mogą być przyczyną chorób nowotworowych, układu nerwowego, pokarmowego, naczyniowego i kostnego. Wykorzystywane są w przemyśle chemicznym, elektromaszynowym, szklarskim, metalurgicznym, farbiarskim, wydobywczym, energetyce jądrowej. Wiele metali, takich jak kobalt, miedź, nikiel, stanowi mikroelementy...

• Triazoloacridone C-1305 abrogates the restriction checkpoint in cells lacking functional p53 and promotes their accumulation in the G2/M phase of the cell cycle

  Publikacja

  • M. Mauer
  • O. Komina
  • A. Składanowski
  • J. Wesierska-Gadek

  - Journal of Experimental Therapeutis and Oncology - Rok 2011

  Triazoloakrydon C-1305, nowy inhibitor topoizomerazy II, wykazuje wysoką aktywość cytotoksyczną wobec komórek wielu typów nowotworów. Komórki mysie pozbawione genu PARP-1 wykazywały nadwrażliwość na działanie tego związku. W pracy testowaliśmy działanie związku wobec dwóch różnych komóek nowotworowych różniących się statusem p53: komórkami białaczki myelocytarnej HL-60 i raka sutka MCF-7. Wykazaliśmy, że inkubacja ze związkiem...

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• Microscopic Imaging to Visualize the Distribution of Dietary Nucleic Acids in Food Products of Various Origins

  Publikacja

  • A. Kościelak
  • Z. Koziara
  • A. Pons Maria
  • R. Płatek
  • A. Bartoszek-Pączkowska

  - Foods - Rok 2023

  Dietary nucleic acids (dietNAs) are being increasingly recognized as important food components with nutritional value. However, the precise dietary recommendations for dietNAs are limited, because established methods for determining the quantity and nutritional role of dietNAs are still lacking. One of the tools to narrow this gap could be microscopic imaging, as a convenient approach to visualize the abundance and distribution...

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• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069382491]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Squamous cell carcinoma, keratinizing, NOS - Unknown, 62 - Tissue image [3300730069389471]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069384391]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069388751]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069387581]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Squamous cell carcinoma, keratinizing, NOS - Unknown, 62 - Tissue image [3300730069384751]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069386901]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069386181]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069383701]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3301001549117101]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069384081]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069382081]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069382121]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069388591]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069383321]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Squamous cell carcinoma, keratinizing, NOS - Unknown, 62 - Tissue image [3300730069381971]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Squamous cell carcinoma, keratinizing, NOS - Unknown, 62 - Tissue image [3300730069383151]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [330073006938321]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069384201]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• LYMPH NODES, Lymph node face, head ,neck - Unknown, 62 - Tissue image [3300730069384551]

  Dane Badawcze

  open access

  • Gdański Uniwersytet Medyczny

  This is the histopathological image of LYMPH NODES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.

• Genetyka człowieka, seminarium

  Kursy Online

  • A. Stanisławska-Sachadyn

  Tematy referatów: 1. Budowa chromosomu i metody badania (metody cytogenetyczne, FISH, CGH) 2. Badanie DNA w medycynie sądowej 3. Genetyczne podstawy odporności immunologicznej 4. Klonowanie zwierząt i ludzi 5. Genetyka nowotworów 6. Katastrofy elektrowni atomowych w Czarnobylu i w Fukushimie a zachorowalność na nowotwory 7. Dziedziczenie epigenetyczne 8. Mikromacierze hybrydyzacyjne  w badaniu ekspresji genów, metylacji...

• A vertical subway, namely, an internal transport system in a 1200 m tall building,

  Publikacja

  • S. Niewitecki

  - Procedia Engineering - Rok 2016

  This article deals with the topic of designing an efficient internal transport system in a 1200 m tall multifunctional building called the “World’s Tower” due to its symbolism and specific architectural form. The building comprises four towers whose bases are 75 m/75 m long each and whose height is 1200 m. Two of the towers are located along the north-south axis while the two others along the east-west one. The towers are joined...

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• Effect of Ethanol Extracts of Propolis (EEPs) against Staphylococcal Biofilm—Microscopic Studies

  Publikacja

  • K. Grecka
  • Z. R. Xiong
  • H. Chen
  • K. Matejczuk
  • R. Worobo
  • P. Szweda

  - Pathogens - Rok 2020

  Staphylococci growing in the form of biofilm exhibit high resistance to a plethora of antibiotics. The aim of the study was to assess the influence of ethanolic extract of propolis (EEPs) on S. epidermidis ATCC 35984 biofilm using fluorescent microscopy. Propidium iodide (PI) and SYTO 9 were used for differentiation of live and dead cells, and calcofluor white was used to stain the extracellular matrix, the self-produced extracellular...

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• Chemical Aspects of Biological Activity of Isothiocyanates and Indoles, the Products of Glucosinolate Decomposition

  Publikacja

  • D. Kołodziejski
  • I. Koss-Mikołajczyk
  • A. Y. Abdin
  • J. Claus
  • A. Bartoszek-Pączkowska

  - CURRENT PHARMACEUTICAL DESIGN - Rok 2019

  There is growing evidence that cancer chemoprevention employing natural, bioactive compounds may halt or at least slow down the different stages of carcinogenesis. A particularly advantageous effect is attributed to derivatives of sulfur-organic phytochemicals, such as glucosinolates (GLs) synthesized mainly in Brassicaceae plant family. GLs are hydrolysed enzymatically to bioactive isothiocyanates (ITC) and indoles, which exhibit strong...

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• Anticancer and antimicrobial properties of novel η6-p-cymene ruthenium(ii) complexes containing a N,S-type ligand, their structural and theoretical characterization

  Publikacja

  • E. Namiecińska
  • B. Sadowska
  • M. Więckowska-Szakiel
  • A. Dołęga
  • B. Pasternak
  • M. Grazul
  • E. Budzisz

  - RSC Advances - Rok 2019

  Ruthenium(II) complexes are lately of great scientific interest due to their chemotherapeutic potential asanticancer and antimicrobial agents. Here we present the synthesis of new pyrazole carbothioamidederivatives and their four arene–ruthenium complexes. The title compounds were characterized with theapplication of IR, NMR, mass spectrometry, elemental analysis and X-ray diffraction. Additionally, for newcomplexes DFT calculations...

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• An MTA-phosphorylase gene discovered in the metagenomic library derived from Antarctic top soil during screening for lipolytic active clones confers strong pink fluorescence in the presence of rhodamine B.

  Publikacja

  • H. Cieśliński
  • A. Długołecka
  • J. Kur
  • M. Turkiewicz

  - FEMS MICROBIOLOGY LETTERS - Rok 2009

  In this work, we present the construction of a metagenomic library in Escherichiacoli using the pUC19 vector and environmental DNA directly isolated fromAntarctic topsoil and screened for lipolytic enzymes. Unexpectedly, the screeningon agar supplemented with olive oil and rhodamine B revealed one unusual pinkfluorescent clone (PINKuv) out of 85 000 clones. This clone harbored a plasmid,pPINKuv, which has an insert of 8317 bp that...

• A cryptic ribosome binding site, false signals in reporter systems and avoidance of protein translation chaos

  Publikacja

  • P. Sachadyn
  • A. Stanisławska-Sachadyn
  • E. M. Kabat
  • A. Zieli Ńska
  • J. Kur

  - JOURNAL OF BIOTECHNOLOGY - Rok 2009

  The expression of reporter gene may be induced by activation of cryptic signalling sequences, as we found while constructing the mutS-lacZ fusion gene. We cloned the Escherichia coli lacZ gene encoding beta-galactosidase into a plasmid vector carrying the Thermus thermophilus mutS gene. The clones expected to produce beta-galactosidase as the C-terminal fusion were selected for the complementation of beta-galactosidase activity...

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