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Wyniki wyszukiwania dla: gene cloning
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REPORT Verification expertise of Detroit Diesel engines 16V149TI on the vessel ORP GEN. PUŁASKI
PublikacjaVerification expertise of propulsion engines for generating sets of the ORP GEN. PUŁASKI - type 16V149TI related to a complaint sent by the shipowner to Shiprepair Yard NAUTA S.A.
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High prevalence of carriers of variant c.1528G>C of HADHA gene causing long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency (LCHADD) in the population of adult Kashubians from North Poland
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Examination of cyp51A and cyp51B expression level of the first Polish azole resistant clinical Aspergillus fumigatus isolate
PublikacjaAspergillus fumigatus is one of the most prevalent airborne fungal pathogens causing infections worldwide. Most A. fumigatus strains are susceptible to azoles, which are administered as the first line therapeutics. However, during last decade the acquired resistance to triazoles by these species has been described. There is a number of publications concerning the examination of clinical A. fumigatus strains from different countries,...
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Does Porcine Oocytes Maturation in Vitro is Regulated by Genes Involved in Transforming Growth Factor Beta Receptor Signaling Pathway?
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Transcriptomic Pattern of Genes Regulating Protein Response and Status of Mitochondrial Activity Are Related to Oocyte Maturational Competence—A Transcriptomic Study
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Identification of predicted genes expressed differentially in pituitary gland tissue of young growing bulls revealed by cDNA-AFLP technique
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Effect of high pressure processing on changes in antibiotic resistance genes expression among strains from commercial starter cultures
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Wykorzystanie komórek Escherichia coli transformowanych genem termostabilnej beta-galaktozydazy z Pyrococcus woesei do wytwarzania galaktozylofruktozy
PublikacjaKomórki rekombinantowej Escherichia coli transformowane genem beta-galaktozydazy z Pyrococcus woesei wykorzystano jako biokatalizator syntezy galaktozylofruktozy. Największą aktywność syntezy wymienionego prebiotyku (7,6 g/L x godz)uzyskano w 80C przy pH 5,4, stosując jako substrat roztwór zawierający 10% laktozy i 20% fruktozy. Aktywność transgalaktozylacji nie ulegała istotnym zmianom podczas sześciu kolejnych szarż produkcyjnych.
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SacNI, an isoschizomer of BanII isolated from streptomyces achromogenes recognizes the 5′-GRGCY/C sequence
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Amplification of erbB-4 oncogene occurs less frequently than that of erbB-2 in primary human breast cancer1Published in conjunction with A Wisconsin Gathering Honoring Waclaw Szybalski on the occasion of his 75th year and 20 years of Editorship-in-Chief of Gene, 10–11 August, 1997, University of Wisconsin, Madison, WI, USA.1
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EPAS1 resistance to miRNA-based regulation contributes to prolonged expression of HIF-2 during hypoxia in human endothelial cells
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Female Fabry disease patients and X-chromosome inactivation
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Cell cycle is disturbed in mucopolysaccharidosis type II fibroblasts, and can be improved by genistein
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Size-controlled synthesis of gold nanoparticles by a novel atmospheric pressure glow discharge system with a metallic pin electrode and a flowing liquid electrode
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Development of direct-current, atmospheric-pressure, glow discharges generated in contact with flowing electrolyte solutions for elemental analysis by optical emission spectrometry
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Simulation of hybridized nanofluids flowing and heat transfer enhancement via 3-D vertical heated plate using finite element technique
PublikacjaThe present study probed the creation of heat energy and concentrating into Newtonian liquids across vertical 3D-heated plates. The role of the Soret and Dufour theories in concentrating and energy formulas is discussed. The role of hybrid nanoparticles is introduced to illustrate particle efciency in terms of solute and thermal energy. It is removed a viscous dissipation process and a changing magnetic feld. The proposed approach...
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The Influence of the Application of EP Additive in the Minimum Quantity Cooling Lubrication Method on the Tool Wear and Surface Roughness in the Process of Turning 316L Steel
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Thermal, electrical, and magnetic properties of Fe2O3–PbO–SiO2 glass prepared by traditional melt-quenching and twin roller fast-cooling methods
PublikacjaIn this study, Fe–Pb–Si oxide glasses containing between 12.5 and 17.5 mol% Fe2O3 were prepared using two different methods comprising traditional melt-quenching and twin roller fast-cooling techniques. The topography and structure of the materials obtained were characterized by X-ray powder diffraction and scanning electron microscopy. All of the materials were found to be amorphous. The topography of most of the glasses comprised...
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Synthesis and application of biomass-derived magnetic biochar catalyst for simultaneous esterification and trans-esterification of waste cooking oil into biodiesel: modeling and optimization
PublikacjaThis work created, characterized, and used a magnetic biochar catalyst that is both eco-friendly and very effective. Sugarcane bagasse was selected as primary raw material for catalyst preparation, because it is renewable and ecofriendly biomass. Cata- lyst created by doping sugarcane bagasse biochar with magnetic material in the form of (FeSO 4 ·7H 2 O). Thermogravimetric Analysis (TGA) and Fourier Transform Infrared spectroscopy...
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In Silico Safety Assessment of Bacillus Isolated from Polish Bee Pollen and Bee Bread as Novel Probiotic Candidates
PublikacjaBacillus species isolated from Polish bee pollen (BP) and bee bread (BB) were characterized for in silico probiotic and safety attributes. A probiogenomics approach was used, and in-depth genomic analysis was performed using a wide array of bioinformatics tools to investigate the presence of virulence and antibiotic resistance properties, mobile genetic elements, and secondary metabolites. Functional annotation and Carbohydrate-Active...
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Influence of the abrasive grain trajectory on the machining of thin-walled cylinder liners of internal combustion engines with variable kinematics of honing
PublikacjaGładzenie ze zmienną kinematyką posiada szereg zalet w porówna-niu do tradycyjnego gładzenia [1-19], takie jak: mniejsze zużycie na-rzędzia [12, 13], niższa temperatura obróbki [13], lepsza jakość po-wierzchni [12, 13] oraz mniejsza odchyłka walcowości [13]. W arty-kule omówiono zalety zmiennej kinematyki gładzenia. Można zało-żyć, że gładzenia ze zmienną kinematyką „VH” zostanie wdrożone wkrótce w przemyśle, np. w produkcji elementów...
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On the coupling of hydride generation with atmospheric pressure glow discharge in contact with the flowing liquid cathode for the determination of arsenic, antimony and selenium with optical emission spectrometry
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Effect of the addition of non-ionic surfactants on the emission characteristic of direct current atmospheric pressure glow discharge generated in contact with a flowing liquid cathode
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Application of Direct Current Atmospheric Pressure Glow Microdischarge Generated in Contact with a Flowing Liquid Solution for Synthesis of Au-Ag Core-Shell Nanoparticles
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Design of self-cleaning and self-disinfecting paper-shaped photocatalysts based on wood and eucalyptus derived cellulose fibers modified with gCN/Ag nanoparticles
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Analysis of Cutting Force and Power Under the Conditions of Minimized Cooling in the Process of Turning AISI-1045 Steel with the Use of the Parameter Space Investigation Method
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Kolorowanie grafów z ograniczeniami na liczbę wierzchołków w określonym kolorze = Graph coloring model with restrictions on cardinalities of vertexes in particular color
PublikacjaW artykule rozważamy problem takiego kolorowania grafów, w którym klasy kolorów mają ograniczoną z góry moc. Zagadnie to znajduje ciekawe zastosowania praktyczne i jest naturalnym uogólnieniem problemu kolorowania grafów. W artykule ustalamy złożoność obliczeniową dla grafów pełnych $r$-dzielnych i dla kilku innych prostych klas grafów oraz dla problemu dwukolorowania.
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Obliczanie czasów chłodzenia spawanych łukowo złączy stali odpornych na korozję = Cooling times calculation for stainless steels arc welded joints.
PublikacjaPrzedstawiono wpływ właściwości cieplnych i parametrów spawania stali odpornych na korozję na czas chłodzenia złącza w zakresie temperatur 800-500oC. Przedstawiono analityczne obliczenia tego czasu. W oparciu o wyniki badań rzeczywistych cykli cieplnych spawania stali typu duplex 2205 opracowano wzór do obliczania czasów chłodzenia złącza 800-500 C.
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Musical Instrument Separation Applied to Music Genre Classification . Separacja instrumentów muzycznych w zastosowaniu do rozpoznawania gatunków muzycznych
PublikacjaThis paper outlines first issues related to music genre classification and a short description of algorithms used for musical instrument separation. Also, the paper presents proposed optimization of the feature vectors used for music genre recognition. Then, the ability of decision algorithms to properly recognize music genres is discussed based on two databases. In addition, results are cited for another database with regard to...
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Evaluation of immune responses in sheep induced by DNA immunization with genes encoding GRA1, GRA4, GRA6 and GRA7 antigens of Toxoplasma gondii
PublikacjaW niniejszej pracy oszacowano odpowiedź immunologiczną owiec szczepionych domięśniowo szczepionkami DNA zawierającymi geny kodujące cztery antygeny granul o dużej gęstości (GAR1, GRA4, GRA6 oraz GRA7) Toxoplasma gondii. Wykazano, iż wykorzystanie DNA plazmidu pGRA7 indukuje znaczący poziom przeciwciał IgG-2 anty-GRA7 oraz IFN-gammma, podczas gdy iniekcja pGRA1, pGRA4 oraz pGRA6 stymuluje odpowiedź przeciwciał IgG1 z ograniczoną...
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Expression of Selected Connexin and Aquaporin Genes and Real-Time Proliferation of Porcine Endometrial Luminal Epithelial Cells in Primary Culture Model
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The Significance of MicroRNAs Expression in Regulation of Extracellular Matrix and Other Drug Resistant Genes in Drug Resistant Ovarian Cancer Cell Lines
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Identification of Powdery Mildew Blumeria graminis f. sp. tritici Resistance Genes in Selected Wheat Varieties and Development of Multiplex PCR
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Microorganisms from starter and protective cultures - Occurrence of antibiotic resistance and conjugal transfer of tet genes in vitro and during food fermentation
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Phosphorylation‐mediated regulation of heat shock response in Escherichia coli
PublikacjaEscherichia coli has two heat shock regulons under the transcriptional control of Esigma(32) and Esigma(E) RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that mutations in a tyrosine phosphatase-encoding gene led to decreased activity of these heat shock regulons. The...
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Phytoecdysteroid containing plants – a source of bioactive compounds with potential anticarcinogenic effects
PublikacjaEcdysteroids are a group of steroids synthesized in inver- tebrates as molting hormones and also in some plants as secondary metabolites acting as a defense against parasites. These compounds do not naturally occur in mammals and are not structurally related to mammalian steroid hor- mones, consequently do not activate mammalian hormone receptors. Ecdysteroids exhibit very low toxicity to mam- mals and to date no serious side effects...
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Peptide Permeases in Candida albicans
PublikacjaAims: The aim of this work was to characterize oligopeptide uptake in Candida albicans mutants in which the genes encoding putative peptide permeases were selectively disrupted. Initial velocities of transport of model oligopeptides, (Ala)2, (Ala)3, (Ala)4, and sensitivity of mutants to antifungal oligopeptides containing N3-(4-methoxyfumaroyl)-L-2,3-diaminopropanoic acid (FMDP) were determined and compared to those of the wild-type...
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Research on water lubricated main shaft bearings in conditions of improper lubrication and cooling conducted on high torque real-life scale bush test rig
PublikacjaWater lubricated polymer bushes find increasingly popular use in the sliding bearings of marine propellers, water turbines and pump shafts. Such popularity is partly due to their simple construction which also means relatively affordable price. In addition, properly designed and installed water lubricated bearings may well last for over a decade. However, in practical experience one may also observe cases of rapid wear...
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Epithelium morphogenesis and oviduct development are regulated by significant increase of expression of genes after long-term in vitro primary culture – a microarray assays
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Fatty Acids Related Genes Expression Undergo Substantial Changes in Porcine Oviductal Epithelial Cells During Long-Term Primary Culture
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The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)
PublikacjaThe aim of this study was to analyze the occurrence of sulfonamide resistance genes (sul1–3) and other genetic elements as antiseptic resistance gene (qacEΔ1) and class 1 and class 2 integrons (int1–2) in the upper layer of substrate and in the effluent of microcosm constructed wetlands (CWs) treating artificial wastewater containing diclofenac and sulfamethoxazole (SMX), which is a sulfonamide antibiotic. The bacteria in the substrate...
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Enhancement of photocatalytic-based processes by mono- and bimetallic (CuPd) rutile loaded nanoparticles for antibiotic resistance genes and facultative pathogenic bacteria removal
PublikacjaThe aim of the study was the strong reduction of facultative pathogenic bacteria (FPB), and clinically relevant antibiotic resistance genes (ARGs) from secondary effluent. To evaluate the ARGs removal efficiency comparative study of individual unit processes and combined AOPs has been performed. The present work investigated: i) removal of selected ARGs, namely blaTEM, ermB, qnrS, tetM, five FPB as well as 16S rDNA and the integrase...
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Simplified method of water cooled exhaust system design.
PublikacjaThe water cooled exhaust systems are not often used onboard ships. Prime goal is to lower temperature of exhaust gas from main propulsion and auxiliary engines to the defined limit. The design of such systems require detailed analysis of propulsion system operational parameters to meet designer expectations. The paper outlines the cooled exhaust system design problems in specific application. The simplified method of evaluation...
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Multicast Traffic Throughput Maximization through Joint Dynamic Modulation and Coding Schemes Assignment, and Transmission Power Control in Wireless Sensor Networks
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The Effect of Posaconazole, Itraconazole and Voriconazole in the Culture Medium on Aspergillus fumigatus Triazole Resistance
PublikacjaTriazoles are the only compounds used as antibiotics in both medicine and agriculture. The presence of triazoles in the environment can contribute to the acquisition of azole resistance among isolates of Aspergillus fumigatus. The objective of this study was to investigate the effect of A. fumigatus exposure to triazoles on susceptibility to these compounds. Seventeen triazole-resistant and 21 triazole-sensitive A. fumigatus isolates...
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Extracellular secretion of Pseudoalteromonas sp. cold-adapted esterase in Escherichia coli in the presence of Pseudoalteromonas sp. components of ABC transport system
PublikacjaEnzymy lipolityczne, głównie lipazy i esterazy, stanowią cenną grupę biokatalizatorów wykorzystywanych w przemyśle. Coraz większe zainteresowanie tą klasą enzymów sprawiło, że poszukuje się alternatywnych źródeł ich pozyskiwania. W celu zbadania wpływu białek transportu ABC Pseudoalteromonas sp. 643A na ekspresję genu, sekrecję i aktywność esterazy EstA z tego mikroorganizmu w systemie ekspresyjnym E. coli skonstruowano układ do...
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Whole-genome sequencing and antimicrobial potential of bacteria isolated from Polish honey
PublikacjaThe aim of this study was the whole-genome analysis and assessment of the antimicrobial potential of bacterial isolates from honey harvested in one geographical location—the north of Poland. In total, 132 strains were derived from three honey samples, and the antimicrobial activity of CFAM (cell-free after-culture medium) was used as a criterion for strain selection and detailed genomic investigation. Two of the tested isolates...
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Bioelectronic nose: Current status and perspectives
PublikacjaA characteristic feature of human and animal organs of smell is the ability to identify hundreds of thousands of odours. It is accompanied by particular smell sensations, which are a basic source of information about odour mixture. The main structural elements of biological smell systems are the olfactory receptors. Small differences in a structure of odorous molecules (odorants) can lead to significant change of odour, which is...
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Production of antimicrobial silver nanoparticles modified by alkanethiol self‐assembled monolayers by direct current atmospheric pressure glow discharge generated in contact with a flowing liquid anode
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Flowing Liquid Anode Atmospheric Pressure Glow Discharge as an Excitation Source for Optical Emission Spectrometry with the Improved Detectability of Ag, Cd, Hg, Pb, Tl, and Zn
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