Filtry
wszystkich: 4743
-
Katalog
- Publikacje 4507 wyników po odfiltrowaniu
- Czasopisma 14 wyników po odfiltrowaniu
- Konferencje 6 wyników po odfiltrowaniu
- Osoby 42 wyników po odfiltrowaniu
- Projekty 2 wyników po odfiltrowaniu
- Kursy Online 27 wyników po odfiltrowaniu
- Wydarzenia 3 wyników po odfiltrowaniu
- Dane Badawcze 142 wyników po odfiltrowaniu
wyświetlamy 1000 najlepszych wyników Pomoc
Wyniki wyszukiwania dla: METAGENOMIC APPROACH
-
Metabarcoding and Metagenomics
Czasopisma -
The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge
PublikacjaA metagenomic approach was used to investigate how the microbial community composition changes when an anammox-based granular sludge reactor is seeded with nitritation-anammox biomass from a wastewater treatment plant. In the seed sample, the abundance of Candidatus Kuenenia stuttgartiensis was similar to Candidatus Jettenia caeni (12.63 vs. 11.68%). This biomass was typical in terms of microbial nitrogen conversion; both ammonia...
-
Going to extremes – a metagenomic journey into the dark matter of life
Publikacja -
The use of fosmid metagenomic libraries in preliminary screening for various biological activities
Publikacja -
Direct cloning of heavy metal resistance genes from metagenomic DNA
Publikacja -
Identification and molecular modeling of a novel lipase from an Antarctic soil metagenomic library.
PublikacjaIn this work, we present the construction of a metagenomic library in Escherichia coli using pUC19 vector and environmental DNA directly isolated from Antarctic topsoil and screened for lipolytic enzymes. The screening on agar supplemented with olive oil and rhodamine B revealed one clone with lipolytic activity (Lip1) out of 11,000 E. coli clones. This clone harbored a plasmid, pLip1, which has an insert of 4722 bp that has been...
-
The Characteristics of New SSB Proteins from Metagenomic Libraries and Their Use in Biotech Applications
Publikacja -
Social Welfare Interdisciplinary Approach
Czasopisma -
Sports Health-A Multidisciplinary Approach
Czasopisma -
SKULL BASE-AN INTERDISCIPLINARY APPROACH
Czasopisma -
Investigation of interaction between xanthene dye rhodamine B and metagenomic-derivated methylthioadenosine phosphorylase RSFP
PublikacjaIn the previous study we found that the expression of the metagenomic - derived methylthioadenosine phosphorylase gene (rsfp) in E. coli cells and the presence of rhodamine B in the growth medium were crucial for the pink fluorescence of one E. coli colony from Antarctic soil metagenomic library (Cieśliński et al., 2009). In this study, rsfp gene expression was induced by an arabinose promoter in an LMG194 E. coli strain and the...
-
A study on the interaction of rhodamine B with methylthioadenosine phosphorylase protein sourced from an Antarctic soil metagenomic library.
PublikacjaThe presented study examines the phenomenon of the fluorescence under UV light excitation (312 nm) of E. coli cells expressing a novel metagenomic-derived putative methylthioadenosine phosphorylase gene, called rsfp, grown on LB agar supplemented with a fluorescent dye rhodamine B. For this purpose, an rsfp gene was cloned and expressed in an LMG194 E. coli strain using an arabinose promoter. The resulting RSFP protein was purified...
-
An examination of an Antarctic soil metagenomic-derivate putative methylthioadenosine phosphorylase gene as a novel reporter gene for promoter trapping
PublikacjaThe rsfp gene was discovered during examination ofan Antarctic soil-derived metagenome library and encodesa bacterial methylthioadenosine phosphorylase (GenBank accession number GQ202582). In a previous study, we found that E. coli colonies expressing the rsfp gene reveal strong pink fluorescence after exposure to UV light when grown on culture medium supplemented with rhodamine B (Cieśliński et al., 2009). In contrast to that,...
-
International Journal of Psychology: A Biopsychosocial Approach
Czasopisma -
Approaches to Aging Control
Czasopisma -
Linguistic Approaches to Bilingualism
Czasopisma -
International Journal of Information Technologies and Systems Approach
Czasopisma -
Increasing conclusiveness of metabonomic studies by cheminformatic preprocessing of capillary electrophoretic data on urinary nucleoside profiles
Publikacja -
Investigation of the C-1311 glucuronidation: an electrochemical approach
Dane BadawczeThis study was undertaken to investigate the glucuronidation of the compound C-1311 (5-diethylaminoethylamino-8-hydroxyimidazoacridinone – the model anticancer acridine derivative) using electrochemistry/mass spectrometry (EC/MS) as a complementary technique to in vitro (liver microsomes) and in silico approaches.
-
A process approach to innovation
PublikacjaOmówiono zarządzanie wiedza zawarta w innowacjach w ujęciu systemowo procesowym.
-
Calibration in Metrological Approach
Publikacja -
Calibration and metrological approach.
PublikacjaPrzedstawiono teraźniejsze podejście do procesu kalibracji. Różnorodność definicji dotyczących pomiarów w chemii analitycznej, nierzadko niekonsekwentnych, może wprowadzić w zakłopotanie. W pracy wyjaśniono pojęcia takie jak: kalibracja, substancje wykorzystywane do kalibracji, walidacja, niepewność materiałów odniesienia. Omówiono również pojęcie spójności pomiarowej w odniesieniu do procesu kalibracji.
-
The OpenMolcas Web: A Community-Driven Approach to Advancing Computational Chemistry
Dane BadawczeThe developments of the open-source OpenMolcas chemistry software environment since spring 2020 are described, with a focus on novel functionalities accessible in the stable branch of the package or via interfaces with other packages. These developments span a wide range of topics in computational chemistry and are presented in thematic sections: electronic...
-
An MTA-phosphorylase gene discovered in the metagenomic library derived from Antarctic top soil during screening for lipolytic active clones confers strong pink fluorescence in the presence of rhodamine B.
PublikacjaIn this work, we present the construction of a metagenomic library in Escherichiacoli using the pUC19 vector and environmental DNA directly isolated fromAntarctic topsoil and screened for lipolytic enzymes. Unexpectedly, the screeningon agar supplemented with olive oil and rhodamine B revealed one unusual pinkfluorescent clone (PINKuv) out of 85 000 clones. This clone harbored a plasmid,pPINKuv, which has an insert of 8317 bp that...
-
System of innovation: a sectoral approach
Publikacjathe article presents a systems approach to innovation as an alternative method of assessing the sector innovativeness. in the article the building blocks of each sectoral system of innovations (ssi) such as a knowledge base, institutions and actors are discussed. moreover, the five models of ssi are presented.
-
An approach for evaluating trust in it infrastructures
PublikacjaW artykule zademonstrowano metodę oceny poziomu zaufania do systemu TI w oparciu o podejście funkcji przekonania.
-
An approach to smart experience management
PublikacjaW pracy przedstawiono procesowe podejście do inteligentnego zarządzania wiedzą zebraną na podstawie przebytych doświadczeń.
-
Collaborative approach to trustworthiness of it infrastructures
PublikacjaArtykuł wprowadza koncepcję ''trust case'' i wyjaśnia jak jest ona używana w celu uzasadnienia zaufania do systemów i infrastruktur IT. W szczególności skoncentrowano się na procesie budowy trust case, wyjasniono jego strukturę oraz podkreślono konieczność włączenia do procesu właściwych udziałowców. Załączono również informację na temat funkcjonalnej struktury narzedzi wspomagających zarządzanie trust case w ramach jego cyklu...
-
Contextual approach to reasoning with rules
PublikacjaIn the paper we present a method of reasoning with Horn rules within a contextual framework. We show that by proper use of a structure of contexts we can obtain partial OWA compliance with no necessity of extending the syntax of underlying Description Logics.
-
An Approach to Trust Case Development
PublikacjaIn the paper we present an approach to the architectural trust case development for DRIVE, the IT infrastructure supporting the processes of drugs distribution and application. The objectives of DRIVE included safer and cheaper drugs distribution and application. A trust case represents an argument supporting the trustworthiness of the system. It is decomposed into claims that postulate some trust related properties. Claims differ...
-
Psychosis-Psychological Social and Integrative Approaches
Czasopisma -
Journal of New Approaches in Educational Research
Czasopisma -
International Journal of Feminist Approaches To Bioethics
Czasopisma -
Hybrid approach to ontology specification and development
PublikacjaIn this chapter a first draft of a hybrid ontology development approach is presented. The context of this method encompasses the multi-agent system equipped with knowledge bases and inferring engine. The process of ontology engineering appears as a complex issue, as it addresses different point-views of both, a client and a modeler with a requirements/system analyst. Regarding this, an approach based on the knowledge preprocessing...
-
A consensus-based approach to the distributed learning
Publikacja -
Risk Modelling for Passages in Approach Channel
Publikacja -
An agent-based approach to ANN training
Publikacja -
An Approach to RBF Initialization with Feature Selection
Publikacja -
An approach to evaluation of arguments in trust cases
PublikacjaTrustworthiness of IT systems can be justified using the concept of a trust case. A trust case is an argument structure which encompasses justification and evidence supporting claimed properties of a system. It represents explicitly an expert's way of assessing that a certain object has certain properties. Trust cases can be developed collaboratively on the basis of evidence and justification of varying quality. They can be complex...
-
Stochastic approach to movements of a multihull on waves
PublikacjaW artykule przedstawiono metodę wyznaczania różniczkowych równań ruchu.Układ równań różniczkowych został przedstwaiony dla ruchów antysymetrycznych jednoski wielokadłubowej (poprzeczne ruchy liniowe, poprzeczne ruchy kątowe, poziome ruchy kątowe). Dla tych ruchów zbudowano stochastyczne równania różniczkowe.
-
Systemic approach to Road Safety Schemes
PublikacjaW artykule przedstawiono koncepcje rozwoju systemu bezpieczeństwa ruchu drogowego na poziomie krajowym oraz na poziomie regionalnym i lokalnym.
-
Determination of resonances by the optimized spectral approach
Publikacja -
Agile Media Management Approach – an analysis
PublikacjaThis article studies media management and demonstrates a fresh perspective on the new approach based on agile methodologies of project management. The analysis sequentially presents a literature overview of media and its management, the characteristics of media projects, agile project management, an analysis of agile approaches and media management along with the proposal of a...
-
Lexicographic Approach to Health Care Optimization
Publikacja -
New approach to the chunk recoginition in Polish
Publikacja -
Finnish Lexical Homonimics – A Quantitative Approach
Publikacja -
Hybrid Approach to Networked Control System
PublikacjaEffcient control of Networked Control System (NCS) is a challenge, as the control methods need to deal with non-deterministic variable delays and data loss. This paper presents a novel hybrid approach to NCS where Model Predictive Control (MPC) is applied as a main controller and implicit switching MPC is used for data transmission control in event-driven shared communication medium, leading to complex control system with active...
-
A Novel Approach to the Assessment of Cough Incidence
PublikacjaIn this paper we consider the problem of identication of cough events in patients suffering from chronic respiratory diseases. The information about frequency of cough events is necessary to medical treatment. The proposed approach is based on bidirectional processing of a measured vibration signal - cough events are localized by combining the results of forward-time and backward-time analysis. The signal is at rst transformed...
-
An approach to determining tinnitus acoustical characteristic
PublikacjaFor many treatment methods, accurate estimation of Tinnitus(ringing in ears) concerning sound type, level, and bandwidth or frequency is inevitable. The proposed way of obtaining Tinnitus parameters is described in this paper. The method employs sound synthesis, aimed at obtaining sound which is closest to perceived Tinnitus. The proposed method assumes running a designed application on a multimedia PC provided with a special graphical...
-
Aggregation of imidazoacridinones - spectroscopic and microcalorimetric approach
PublikacjaImidazoacridinones (IA) belong to a group of polycyclic heteroaromatic antitumor compounds. IA create the aggregated form in water solution. Self-association of substances is a very important phenomenon in many fields of applied chemistry. It is also fundamental model reaction of many kinds of molecular interactions such as the micelle formation of amphiphilic substances and the binding of small organic molecules to macromolecules....