Search results for: site-directed mutagenesis
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Intermolecular Hydrogen Bonding Directed by Aryl–Perfluoroaryl π–π Stacking Interactions
PublicationThe crystal structures of five compounds capable of forming self-complementary hydrogen bonds but crystallizing as catemers or creating more complex crystal structures were compared with their complexes prepared by cocrystallization with perfluoroaryl compounds. The results of X-ray diffraction revealed that in all the cases the π–π stacking interactions caused reorganization of hydrogen bonds and induced creation of the expected...
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Active Site Architecture and Reaction Mechanism Determination of Cold Adapted beta-D-galactosidase from Arthrobacter sp. 32cB
PublicationArthbetaDG is a dimeric, cold-adapted beta-D-galactosidase that exhibits high hydrolytic and transglycosylation activity. A series of crystal structures of its wild form, as well as its ArthbetaDG_E441Q mutein complexes with ligands were obtained in order to describe the mode of its action. The ArthbetaDG_E441Q mutein is an inactive form of the enzyme designed to enable observation of enzyme interaction with its substrate. The...
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Praktyka budowlana/Construction on site training 2022/2023
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A new approach to modeling of selected human respiratory system diseases, directed to computer simulations
PublicationThis paper presents a new versatile approach to model severe human respiratory diseases via computer simulation. The proposed approach enables one to predict the time histories of various diseases via information accessible in medical publications. This knowledge is useful to bioengineers involved in the design and construction of medical devices that are employed for monitoring of respiratory condition. The approach provides the...
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Divulging the anti-acetylcholinesterase activity of Colletotrichum lentis strain KU1 extract as sustainable AChE active site inhibitors
PublicationAlzheimer’s disease (AD), also called senile dementia is a neurodegenerative disease seen commonly in the elderly and is characterised by the formation of β-amyloid plaques and neurofbrillary tangles (NFT). Though a complete understanding of the disease is lacking, recent studies showed the role of the enzyme acetylcholinesterase (AChE) in pathogenesis. Finding new lead compounds from natural sources has always been a quest for...
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New Pr3+ site in β-SiAlON red phosphor
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Magdalena Apollo dr inż.
People2017 - PhD in Civil Engineering, thesis: Risk management in construction investments related to urban regeneration projects, Gdansk University of Technology IX 2012–VI 2013 - Postgraduate Studies at Gdynia Maritime University: Research Project Management (IPMA D Certificate) 2010 – MSc in Management and Marketing, Gdansk University of Technology 2007 – MSc in Civil Engineering, Gdansk University of Technology 2007-2010 - structural...
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MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS
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Research, consulting and educational offer directed to Pomeranian province society in the area of hydro and environmental engineering.
PublicationPrzedstawiono skład osobowy, Laboratoria i Katedry Wydziału zajmujące się tematyką inżynierii wodnej i inżynierii środowiska. Omówiono zakres i tematykę prac i oferty naukowej i dydaktycznej. Podano przykłady zrealizowanych obiektów oraz propozycję współpracy na przyszłość dla województwa pomorskiego.
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Production of the anticancer drug taxol by the endophytic fungus Epicoccum nigrum TXB502: enhanced production by gamma irradiation mutagenesis and immobilization technique
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Integrating web site services into application through user interface
PublicationThe issue of integrating applications which are only accessible through visual user interface is not thoroughly researched. Integration of web applications running remotely and controlled by separate organizations becomes even more complicated, as their user interface can display differently in different browsers or change without prior notification as a result of application maintenance. While possible, it is generally not common...
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Ce site dilution effects in the antiferromagnetic heavy fermion CeIn3
PublicationLa-doped intermetallic single crystals of Ce1−xLaxIn3 were synthesized via an In self-flux method throughout the entire range (x = 0–1). The prototypical heavy-fermion compound CeIn3 shows an antiferromagnetic phase transition at 10.1 K and becomes superconducting near a critical pressure where TN is completely suppressed. As the La concentration increases, Ce moments are diluted, and the lattice constant increases linearly, satisfying Vegard’s...
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Prediction of Pile Shaft Capacity in Tension Based on Some Direct CPT Methods—Vistula Marshland Test Site
PublicationThis paper presents different CPT methodologies for the prediction of the pile shaft resistance in tension on the example of three reference screw piles of the Jazowa test site in Poland. The shaft capacity was estimated based on the cone resistance, sleeve friction and CPT excess pore water pressure. Three piles with diameter 0.4 m and the length varied from 8 m to 14.6 m were subjected to static load tests in tension. Their...
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Prediction of pile shaft capacity in tension based on some direct CPT methods – Vistula Marshland test site
PublicationThis paper presents different CPT methodologies for the prediction of the pile shaft resistance in tension on the example of three reference screw piles of the Jazowa test site in Poland. The shaft capacity was estimated based on the cone resistance, sleeve friction and CPT excess pore water pressure. Three piles with a diameter of 0.4 m and the length...
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Highly Conserved Homotrimer Cavity Formed by the SARS-CoV-2 Spike Glycoprotein: A Novel Binding Site
PublicationAn important stage in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) life cycle is the binding of the spike (S) protein to the angiotensin converting enzyme-2 (ACE2) host cell receptor. Therefore, to explore conserved features in spike protein dynamics and to identify potentially novel regions for drugging, we measured spike protein variability derived from 791 viral genomes and studied its properties by molecular...
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Unlocking the biosynthetic potential of Penicillium roqueforti for hyperproduction of the immunosuppressant mycophenolic acid: Gamma radiation mutagenesis and response surface optimization of fermentation medium
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Site environment type – The main factor of urban road dust toxicity?
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Sensitivity analysis of the contractor’s financial effects achieved on a single building site
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Some remarks on problem of site characterisation - a subjective experience on theory and practice
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Transumbilical laparoendoscopic single-site total mesorectal excision for rectal carcinoma
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The Baltic Sea as a dumping site of chemical munitions and chemical warfare agents
PublicationW pracy przedstawiono problem składowania w Morzu Bałtyckim aminucji chemicznej i bojowych środków trujących. Przedstawiono typy oraz charakterystykę BST składowanych na dnie Morza Bałtyckiego. Omówiono potencjalne zagrożenia dla środowiska abiotytcznego oraz dla organizmów żywych związanych z tego typu składowiskami. Przedstawiono również podstawowe informacje o procedurach analitycznych, które mogą być wykorzystane do wykrywania...
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Density functional theory calculations on entire proteins for free energies of binding: Application to a model polar binding site
PublicationIn drug optimization calculations, the molecular mechanics Poisson-Boltzmann surface area (MM-PBSA) method can be used to compute free energies of binding of ligands to proteins. The method involves the evaluation of the energy of configurations in an implicit solvent model. One source of errors is the force field used, which can potentially lead to large errors due to the restrictions in accuracy imposed by its empirical nature....
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Temat 2: Site-Specific, Land Art, Interwencja - Projekt formy przestrzennej w skali 1:1
e-Learning CoursesKrótki opis problematyki zajęć: Miejsce, przestrzeń, czas, odczucie, emocje, twórcze przetworzenie. Zadanie projektowe odnosi się do działań artystycznych i projektowych z obszaru Site-Specific, Land Art oraz Interwencja w różnych lokalizacjach. W zadaniu powinna być uwzględniona specyfika przestrzenna i historyczna Kampusu Politechniki Gdański. Należy zaprojektować i zrealizować obiekt lub zespół obiektów w skali 1:1 zintegrowanych...
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Metal ion directed template synthesis using 2-acetyl-1,3-indandione and ethylenediamine: steric and electronic restrictions
PublicationA template synthesis using 2-acetyl-1,3-indandione and ethylenediamine results in formation of octahedral Ni complex, whose crystal structure has been resolved using single crystal X-ray diffraction. The structure indicates that only exocyclic enolic oxygen atom of the triketone takes part in the condensation with ethylenediamine.
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Surgical Site Infection after Breast Surgery: A Retrospective Analysis of 5-Year Postoperative Data from a Single Center in Poland
PublicationBackground and Objectives: Surgical site infection (SSI) is a significant complication of non-reconstructive and reconstructive breast surgery. This study aimed to assess SSI after breast surgery over five years in a single center in Poland. The microorganisms responsible for SSI and their antibiotic susceptibilities were determined. Materials and Methods: Data from 2129 patients acquired over five years postoperatively by the...
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Modelling of temperature field of flat boiling section with active single nucleation site
PublicationZaprezentowano główne założenia wybranych modeli wrzenia dla pojedynczego centrum nukleacji. Przedstawiony model numeryczny dotyczy zagadnienia dwuwymiarowego, osiowo-symetrycznego, nieizotropowego przewodzenia ciepła dla warunków brzegowych pierwszego, drugiego i trzeciego rodzaju. Warunek brzegowy drugiego rodzaju reprezentuje gęstość strumienia ciepła połączoną z generacją pęcherzy z pojedynczego centrum nukleacji.
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Synthesis, crystal structure and electrical properties of A-site cation ordered BaErMn2O5 and BaErMn2O6
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Transporting false doors at the construction site of the Temple of Hatshepsut at Deir el-Bahari
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Self-assembly of zinc and cobalt complexes mimicking active site of alcohol dehydrogenase
PublicationPo raz pierwszy zsyntetyzowano obojętne kompleksy cynku i kobaltu naśladujące strukturę centrum aktywnego dehydrogenazy alkoholowej z wodą jako dodatkowym ligandem. Jakość otrzymanych kryształów pozwoliła na szczegółową analizę wewnątrzcząsteczkowych wiązań wodorowych w kryształach tych związków. Wnioski potwierdzono za pomocą spektroskopii FT-IR w ciele stałym.
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The new fluorogenic substrates of neutrophil proteinase 3 optimized in prime site region
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How acidic amino acid residues facilitate DNA target site selection
PublicationDespite the negative charge of the DNA backbone, acidic residues (Asp/Glu) commonly participate in the base readout, with a strong preference for cytosine. In fact, in the solved DNA/protein structures, cytosine is recognized almost exclusively by Asp/Glu through a direct hydrogen bond, while at the same time, adenine, regardless of its amino group, shows no propensity for Asp/Glu. Here, we analyzed the contribution of Asp/Glu...
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Tailoring structural properties of lanthanum orthoniobates through an isovalent substitution on the Nb-site
PublicationTetragonal polymorph of lanthanum orthoniobate can be stabilized to room temperature by the substitution of Nb with an isovalent element. LaNb1-xAsxO4 (0 < x ≤ 0.3), where As is an element stabilizing tetragonal structure, were successfully synthesized with combined co-precipitation and solid-state reaction method. The phase transition temperature, above which the material has tetragonal structure, decreases linearly with increasing...
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Reclamation of phosphogypsum deposition site Wiślinka near Gdańsk with municipal sewage sludge.
PublicationOmówiono zastosowanie osadów ściekowych do rekultywacji składowiska fosfogipsów w Wiślince k. Gdańska. Głównie pojawiają się problemy ze stabilizacją stoków oraz z sukcesją roślinności i zwalczaniem erozji wiatrowej.
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Adolfo Poma
PeopleI am the group leader of the computational modelling of biomolecules divison at the institute of Fundamental Technological Research Polish Academy of Sciences in Poland. My group was hosted between 2021-2022 by International Centre for Research on Innovative Bio-based Materials (ICRI-BioM). In 2008, I got a Master degree in computational physics from the State University of Campinas, Brazil. Then I moved to Germany and under the...
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Mutation Research - Genetic Toxicology and Environmental Mutagenesis
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A novel source of the cardiac glycoside digoxin from the endophytic fungus Epicoccum nigrum : isolation, characterization, production enhancement by gamma irradiation mutagenesis and anticancer activity evaluation
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Cable stayed bridge over Vistula river in Plock. Dynamic analysis and site test
PublicationBudowę mostu przez rzekę Wisłę w Płocku ukończono w 2006 roku. Główne przęsło o długości 375 m zaprojektowano jako stalową konstrukcję podwieszoną. W celu weryfikacji konstrukcji zbudowano przestrzenny model MES. Badano zachowanie się konstrukcji mostu pod wpływem statycznego i dynamicznego obciążenia. W dalszym etapie przeprowadzono badania in - situ weryfikujące analizy numeryczne oraz założenia projektowe. Na bazie modelu MES...
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Distal pancreatic resection via laparo-endoscopic single site surgery – development of the technique
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The Late Neolithic sepulchral and ritual place of site 14 in Kowal (Kuyavia, Central Poland)
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Folate-directed zinc (II) phthalocyanine loaded polymeric micelles engineered to generate reactive oxygen species for efficacious photodynamic therapy of cancer
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Identification of sustainable trypsin active‐site inhibitors from Nigrospora sphaerica strain AVA‐1
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Optimization of Transport Properties of A-Site Ordered LnBa1-xSrxCo2-yFeyO5+ Perovskite-Type Cathode Materials
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Defining a novel domain that provides an essential contribution to site-specific interaction of Rep protein with DNA
PublicationAn essential feature of replication initiation proteins is their ability to bind to DNA. In this work, we describe a new domain that contributes to a replication initiator sequence-specific interaction with DNA. Applying biochemical assays and structure prediction methods coupled with DNA–protein crosslinking, mass spectrometry, and construction and analysis of mutant proteins, we identified that the replication initiator of the...
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Mesolithic fishery in the Polish Lowland. Fish remains from the Site 7 at Krzyż Wielkopolski, Poland
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Correction of far-field measurements obtained in non-anechoic test site
Open Research DataThe dataset contains raw and processed measurements of radiation pattern characteristics performed in non-anechoic regime for two geometrically small antenna structures: a spline-parameterized Vivaldi structure and a compact spline-based monopole. The responses have been obtained at the selected frequencies of interest as a function of mentioned structures...
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Emergence of Aggregation Induced Emission (AIE), Room-Temperature Phosphorescence (RTP), and Multistimuli Response from a Single Organic Luminogen by Directed Structural Modification
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Enzyme immobilization on carriers as a way of directed modification of biocatalysator properties Immobilizacja enzymów na nośnikach sposobem na ukierunkowaną modyfikację właściwości biokatalizatorów
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Highly Conserved Homotrimer Cavity Formed by the SARS-CoV-2 Spike Glycoprotein: A Novel Binding Site
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A Single Mutation in a Tunnel to the Active Site Changes the Mechanism and Kinetics of Product Release in Haloalkane Dehalogenase LinB*
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Synthesis and SAR Studies of Antibacterial Peptidyl Derivatives Based upon the Binding Site of Human Cystatin C
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