Search results for: toxicology
-
Is the presence of Central European strains of Raphidiopsis (Cylindrospermopsis) raciborskii a threat to a freshwater fish? An in vitro toxicological study in common carp cells
Publication -
Free-ranging domestic cats are characterized by increased metal content in reproductive tissues
Publication -
The altered platelet mineral ratios in pregnancy complicated with intrauterine growth restriction
Publication -
Diclofenac and atrazine restrict the growth of a synchronous Chlamydomonas reinhardtii population via various mechanisms
Publication -
Combination of ABT-737 and resveratrol enhances DNA damage and apoptosis in human T-cell acute lymphoblastic leukemia MOLT-4 cells
Publication -
Lifespan of etoposide-treated human neutrophils is affected by antioxidant ability of quercetin
Publication -
Correlations between levels of dioxins and PCBs in environment samples and wild boar muscles
Publication -
Blood concentrations of a new psychoactive substance 4-chloromethcathinone (4-CMC) determined in 15 forensic cases
PublicationPurpose: The 4-chloromethcathinone (4-CMC) is a synthetic derivative of cathinone and belongs to new psychoactive substances. Neither data on the effects of 4-CMC on the human body, nor on nontoxic, toxic and lethal concentrations in biological materials have been published in the literature. This paper describes the results of an analysis of the blood concentrations of 4-CMC determined in 15 forensic cases related to nonfatal...
-
Improved cytotoxicity and preserved level of cell death induced in colon cancer cells by doxorubicin after its conjugation with iron-oxide magnetic nanoparticles
PublicationA promising strategy for overcoming the problem of limited efficacy in antitumor drug delivery and in drug release is the use of a nanoparticle-conjugated drug. Doxorubicin (Dox) anticancer chemotherapeutics has been widely studied in this respect, because of severe cardiotoxic side effects. Here, we investigated the cytotoxic effects, the uptake process, the changes in cell cycle progression and the cell death processes in the...
-
Serum bisphenol A concentrations correlate with serum testosterone levels in women with polycystic ovary syndrome
PublicationThe aim of this study was to determine serum bisphenol A (BPA) concentrations using high performance liquid chromatography with tandem mass spectrometry (HPLC–MS/MS) in women with polycystic ovary syndrome (PCOS) (n=106, age range 18–40 yrs) and to evaluate its potential impact on their hormonal and metabolic profile. The control group consisted of age- and BMI-matched 80 eumenorrheic women with no clinical or biochemical hyperandrogenism....
-
Bisphenol A analogues and metabolic syndrome in women with polycystic ovary syndrome
PublicationBisphenols (BPs) have become a chemical group of special interest due to their ability to interfere with the endocrine system and their ubiquitous presence in the environment. As some of them possess mild estrogenic and anti-androgenic effects, they might be associated with the diagnosis of polycystic ovary syndrome (PCOS). Acting on multiple tissues, BPs exposure may lead to metabolic derangements characteristic for metabolic...
-
The extended version of restriction analysis approach for the examination of the ability of low-molecular-weight compounds to modify DNA in a cell-free system
PublicationOne of the primary requirements in toxicology is the assessment of ability of chemicals to induce DNA covalent modification. There are several well-established methods used for this purpose such as 32P-Postlabeling or HPLC-MS. However, all of these approaches have difficult to overcome limitations, which prevents their use in genotoxin screening. Here, we describe the simple protocol exploiting specificity of restriction enzymes...
-
Fatal N-Ethylhexedrone Intoxication
PublicationN-Ethylhexedrone [2-(ethyloamino)-1-phenylhexan-1-one; α-ethylaminohexanophenone; NEH] is one of the most recent synthetic cathinones that appeared on the illegal market in late 2015. The majority of information concerning the model of consumption of NEH and its impact on the body originates only from self-reports from grey literature websites and drug forums. There are only limited data associated with the concentrations of NEH...
-
The effect of oxidized cholesterol on barrier functions and IL-10 mRNA expression in human intestinal epithelium co-cultured with dendritic cells in the transwell system
Publication -
Letter to the editor on the article “Alteration of redox status by commonly used antimalarial drugs in the north-western region of Nigeria” by Muhammad A et al.
Publication -
Acute and subacute (28-Day) toxicity studies of ionic liquid, didecyldimethyl ammonium acesulfamate, in rats
Publication -
Cytotoxicity, acute and subchronic toxicity of ionic liquid, didecyldimethylammonium saccharinate, in rats
Publication -
Effect of sweet grass extract against oxidative stress in rat liver and serum
Publication -
Chromatographic Examinations of Tea's Protection Against Lipid Oxidative Modifications
Publication -
Effect of novel dinuclear platinum(II) complexes on redox status of MOLT-4 leukemic cells
Publication -
Cadmium, arsenic, selenium and iron– Implications for tumor progression in breast cancer
Publication -
Crosstalk between liver antioxidant and the endocannabinoid systems after chronic administration of the FAAH inhibitor, URB597, to hypertensive rats
Publication -
Antioxidant properties of black tea in alcohol intoxication
Publication -
The bioactivity of processed garlic (Allium sativum L.) as shown in vitro and in vivo studies on rats
PublicationCzosnek w trakcie procesu obróbki traci znaczną część swojej bioaktywności. Właściwości przeciwutleniające zmierzone metodą FRAP i DPPH stwierdzono na najwyższym poziomie w przypadku czosnku surowego oraz po krótkim okresie gotowania. Badania na szczurach poddanych diecie z dodatkiem krótkogotowanego czosnku potwierdziły, że proces ten nie zaburza właściwości przeciwutleniających surowego czosnku. U zwierząt stwierdzono poprawę...
-
Protective effect of desloratadine against oxidative stress in human erythrocytes in vitro
Publication -
Dietary quercetin does not affect pituitary lutenizing hormone (LH) expression and has no uterotropic effects in ovariectomized Sprague–Dawley rats
Publication -
The p53-mediated cytotoxicity of photodynamic therapy of cancer: Recent advances
Publication -
Fatal intoxication with hydrocarbons in deltamethrin preparation
Publication -
Influence of selenium and/or magnesium on alleviation alcohol induced oxidative stress in rats, normalization function of liver and changes in serum lipid parameters
Publication -
Fulminant hepatic failure in woman with iron and non-steroidal anti-inflammatory drug intoxication
Publication -
Effect of resveratrol analogue, DMU-212, on antioxidant status and apoptosis-related genes in rat model of hepatocarcinogenesis
Publication -
Health threat associated with Caucasian giant hogweeds: awareness among doctors and general public in Poland
Publication -
Zinc oxide nanoparticles toxicity toDaphnia magna: size-dependent effects and dissolution
Publication -
Cylindrospermopsin: Water-linked potential threat to human health in Europe
Publication -
Estimation of smokers' exposure to mercury from combustible tobacco products, based on the approach used in food consumers’ exposure estimation
PublicationSmoking has been known to mankind for centuries, but it is only in recent decades that much attention has been paid to the harmfulness of this habit. Mercury inhalation is particularly dangerous in this respect and smoking creates extremely favorable conditions for the emission and targeted delivery of this element into the lungs. Despite this fact, a lack of a clear method for estimating the exposure of tobacco consumers to mercury...
-
Comet assay-based methods for measuring DNA repair in vitro; estimates of inter- and intra-individual variation.
PublicationZdolność do naprawy uszkodzeń DNA jest jednym z najważniejszych wyznaczników podatności na choroby nowotworowe. Dlatego też użytecznym staje się możliwość pomiaru stopnia naprawy DNA w próbkach materiału badawczego pobranego od ochotników. Celem pracy było, po pierwsze, opracowanie w warunkach in vitro prostej metody (z wykorzystaniem techniki testu kometowego), w której naprawa DNA odbywa się poprzez wycinanie nukleotydów (NER)....
-
Volatile Compound Emissions from Stereolithography Three-Dimensional Printed Cured Resin Models for Biomedical Applications
PublicationStereolithography three-dimensional printing is used increasingly in biomedical applications to create components for use in healthcare and therapy. The exposure of patients to volatile organic compounds (VOCs) emitted from cured resins represents an element of concern in such applications. Here, we investigate the biocompatibility in relation to inhalation exposure of volatile emissions of three different cured commercial resins...
-
Study of Bioreductive Anticancer Agent RH-1-Induced Signals Leading the Wild-Type p53-Bearing Lung Cancer A549 Cells to Apoptosis
Publication -
The changes of antioxidant defense system caused by quercetin administration do not lead to DNA damage and apoptosis in the spleen and bone marrow cells of rats
Publication -
Prolonged Quercetin Administration Diminishes the Etoposide-Induced DNA Damage in Bone Marrow Cells of Rats
Publication -
Hepatoprotective effect of quercetin: From chemistry to medicine
Publication -
The dose-dependet influence of zinc and cadmium contamination of soil on their uptake and glucosinolate content in white cabbage (Brassica oleracea var. capitata f. alba)
PublicationThe relationship between the ability to accumulate heavy metals (represented by Cd and Zn) and to synthesize bioactive compounds (represented by glucosinolates[GLS]) was investigated in two cabbage cultivars. Plants were grown in the greenhouse of a phytotron under controlled conditions in soils spiked with two different Zn or Cd concentrations. The measurements of Cd and Zn contents in soil and cabbage (leaf) samples were performed...
-
Gentiana asclepiadea exerts antioxidant activity and enhances DNA repair of hydrogen peroxide- and silver nanoparticles-induced DNA damage
PublicationExposure to high levels of different environmental pollutants is known to be associated with induction of DNA damage in humans. Thus DNA repair is of great importance in preventing mutations and contributes crucially to the prevention of cancer. In our study we have focused on quantitative analysis of Gentiana asclepiadea aqueous or methanolic extracts obtained from flower and haulm, their antioxidant potency in ABTS post-column...
-
Novel Resveratrol-Based Substrates for Human Hepatic, Renal, and Intestinal UDP-Glucuronosyltransferases
PublicationTrans-Resveratrol (tRes) has been shown to have powerful antioxidant, anti-inflammatory, anti-carcinogenic, and anti-aging properties; however, its use as a therapeutic agent is limited by its rapid metabolism into its conjugated forms by UDP-Glucuronosyltransferases (UGTs). The aim of the current study was to test the hypothesis that the limited bioavailability of tRes can be improved by modifying its structure to create analogs...
-
Genotoxicity of selected pharmaceuticals, their binary mixtures, and varying environmental conditions – study with human adenocarcinoma cancer HT29 cell line
PublicationPharmaceutical residues are present in the environment in mixtures and their adverse effects may also result from interactions that occur between compounds. Studies presented in this work focus on genotoxicity of pharmaceuticals from different therapeutic groups in mixtures and in individual solutions impacted with different environmental conditions assessed using comet assay (alkaline approach). Binary mixtures of pharmaceuticals...
-
Application of Ion Chromatography for the Determination of Inorganic Ions, Especially Thiocyanates, in Human Semen Samples as Biomarkers of Environmental Tobacco Smoke Exposure.
PublicationPalenie tytoniu stanowi istotne źródło zanieczyszczenia powietrza wewnętrznego. Szkodliwość palenia tytoniu potwierdzają zarówno badania doświadczalne, jak i opracowania epidemiologiczne. Celem przeprowadzonych prac badawczych było uzyskanie informacji o poziomie zawartości jonów nieorganicznych, a zwłaszcza jonów rodankowych (jako biomarkerów narażenia na szkodliwe składniki obecne w środowiskowym dymie tytoniowym) w próbkach...
-
Therapeutic Potential of Carnosine and Its Derivatives in the Treatment of Human Diseases
PublicationDespite significant progress in the pathogenesis, diagnosis, treatment, and prevention of cancer and neurodegenerative diseases, their occurrence and mortality are still high around theworld. The resistance of cancer cells to the drugs remains a significant problem in oncology today,while in the case of neuro-degenerative diseases, therapies reversing the process are still yet to be found. Furthermore, it is important to seek new...
-
Long-Term Stability of Benzodiazepines and Z-Hypnotic Drugs in Blood Samples Stored at Varying Temperatures
PublicationBenzodiazepines (BZDs) and Z-drugs are among the most commonly prescribed pharmaceuticals in the world and are considered standard care for various mental illnesses and for treatment of sleeping and anxiety disorders, alcohol withdrawal, muscle spasms and epilepsy. Some BZDs are not allowed as pharmaceuticals in many countries, and they are used as "designer benzodiazepines" (DBZDs). All these compounds are typically screened in...
-
Exposure Assessment to Glyphosate of Two Species of Annelids
Publication -
Bacterial luminescence test screening of highly polluted areas in the Odra River
PublicationW pracy przedstawiono wyniki badania ekotoksyczności wód i osadów z dorzecza Odry w obrazie testu z zastosowaniem bakterii Vibrio Fischerii. Badania przesiewowe wskazują, że silnie zanieczyszczone są miejsca w okolicy Brzegu Dolnego, ujścia Nysy Kłodzkiej i Małej Panwi.