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Search results for: bee proteins
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BALCONY: an R package for MSA and functional compartments of protein variability analysis
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Myotilin, a New Topotecan Resistant Protein in Ovarian Cancer Cell Lines
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C-reactive protein as a diagnostic and prognostic factor in inflammatory bowel diseases
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TOLLIP Protein Expression Predicts Unfavorable Outcome in Renal Cell Carcinoma
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Stability of delay induced oscillations in gene expression of Hes1 protein model
PublicationW pracy prezentowana jest matematyczna analiza modelu ekspresji genu białka Hes1. Rozważamy układ równań z dwoma opóźnieniami - w produkcji białka Hes1 oraz jego mRNA. Dowodzimy istnienia i jednoznaczności oraz nieujemności rozwiązań. Pokazujemy istnienie dokładnie jednego dodatniego stanu stacjonarnego. Wykazujemy, że stabilność stanu stacjonarnego zależy od sumy opóźnień. Podajemy warunek dostateczny na wystąpienie bifurkacji...
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Novel primosomal protein B from thermophilic bacterium Thermoanerobacter tengcongensis
PublicationWe report the identification and characterization of theprimosomal protein B (PriB) from thermophilic bacteriumThermoanerobacter tengcongensis (TtePriB). It is the largestknown bacterial PriB protein consisting 216 amino acidresidues with a calculated molecular mass of 25 kDa. Surprisingly,it is functional as monomer containing two single-stranded DNA binding domain (OB-fold) and it is thecompletely new kind structure of SSB protein....
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Relationship between Milk Protein Polymorphism and Selected Cows’ Reproductive Indices
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Modified Peptide Molecules As Potential Modulators of Shelterin Protein Functions; TRF1
PublicationIn this work, we present studies on relatively new and still not well-explored potential anticancer targets which are shelterin proteins, in particular the TRF1 protein can be blocked by in silico designed "peptidomimetic" molecules. TRF1 interacts directly with the TIN2 protein, and this protein-protein interaction is crucial for the proper functioning of telomere, which could be blocked by our novel modified peptide molecules....
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Impact of AlphaFold on structure prediction of protein complexes: The CASP15‐CAPRI experiment
PublicationWe present the results for CAPRI Round 54, the 5th joint CASP-CAPRI protein assembly prediction challenge. The Round offered 37 targets, including 14 homodimers, 3 homo-trimers, 13 heterodimers including 3 antibody–antigen complexes, and 7 large assemblies. On average 70 CASP and CAPRI predictor groups, including more than 20 automatics servers, submitted models for each target. A total of 21 941 models submitted by these groups...
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Changes in mussel Mytilus galloprovincialis protein profile as a reaction of water pollution
PublicationW pracy przedstawiono zmiany w profilu białkowym małża Mytilus galloprovincialis jako odpowiedź na zanieczyszczenia wody. Określono pojemność przeciwutleniającą z wykorzystaniem testu TEAC. Stwierdzono różnice w poziomie inhibicji w białku pochodzącym od małży zebranych z regionów zanieczyszczonych w stosunku do małży pochodzących z regionów czystych.Zawartość cynku i kadmu w białkach małży pochodzących z regionów zanieczyszczonych...
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Evolution towards simplicity in bacterial small heat shock protein system
PublicationEvolution can tinker with multi-protein machines and replace them with simpler single-protein systems performing equivalent functions in an equally efficient manner. It is unclear how, on a molecular level, such simplification can arise. With ancestral reconstruction and biochemical analysis, we have traced the evolution of bacterial small heat shock proteins (sHsp), which help to refold proteins from aggregates using either two...
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A coarse‐grained approach to NMR ‐data‐assisted modeling of protein structures
PublicationThe ESCASA algorithm for analytical estimation of proton positions from coarse-grained geometry developed in our recent work has been implemented in modeling protein structures with the highly coarse-grained UNRES model of polypeptide chains (two sites per residue) and nuclear magnetic resonance (NMR) data. A penalty function with the shape of intersecting gorges was applied to treat ambiguous distance restraints, which automatically...
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Water-mediated influence of a crowded environment on internal vibrations of a protein molecule
PublicationThe influence of crowding on the protein inner dynamics is examined by putting a single protein molecule close to one or two neighboring protein molecules. The presence of additional molecules influences the amplitudes of protein fluctuations. Also, a weak dynamical coupling of collective velocities of surface atoms of proteins separated by a layer of water is detected. The possible mechanisms of these phenomena are described....
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Mathematical analysis of a generalised p53-Mdm2 protein gene expression model
PublicationWe propose the generalisation of the p53-Mdm2 protein gene expression model introduced by Monk (2003). We investigate the stability of a unique positive steady state and formulate conditions which guarantee the occurrence of the Hopf bifurcation. We show that oscillatory behaviour can be caused not only by time lag in protein transcription process, but also can be present in the model without time delay. Moreover, we investigate...
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Influence of milk, milk fractions and milk proteins on the growth and viability of mastitis-causing Staphylococcus aureus strain
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Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF
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Impact of the depletion of high-abundance proteins in blood plasma/serum on the proteome of these media in growing farm animals
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Fibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeasts
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Evolution of functionally important compartments of proteins – what can we learn from studying epoxide hydrolases?
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Photoactive Protochlorophyllide-Enzyme Complexes Reconstituted with PORA, PORB and PORC Proteins of A. thaliana: Fluorescence and Catalytic Properties
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The Major Heat Shock Proteins, Hsp70 and Hsp90, in 2-Methoxyestradiol-Mediated Osteosarcoma Cell Death Model
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Combining the MARTINI and Structure-Based Coarse-Grained Approaches for the Molecular Dynamics Studies of Conformational Transitions in Proteins
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Borrelia burgdorferi BmpA-BBK32 and BmpA-BBA64: New Recombinant Chimeric Proteins with Potential Diagnostic Value
PublicationCurrently, the diagnosis of Lyme disease is based mostly on two-tiered serologic testing. In the new generation of immunoenzymatic assays, antigens comprise whole-cell lysates of members of the Borrelia burgdorferi sensu lato (s.l.) species complex, with the addition of selected recombinant proteins. Due to the high diversity of members of the B. burgdorferi s.l. genospecies and the low degree of conservation among the amino acid...
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Novel single-stranded DNA-binding proteins from extreme psychrophilic bacterium Psychromonas ingrahamii 37
PublicationWe report the identification and characterization of the single-stranded DNA binding protein (SSB) from extreme psychrophilic bacterium Psychromonas ingrahamii 37 (PinSSB) that grows expotentially at -12°C and may well grow at even lower temperatures. PinSSB is one of the largest known bacterial SSB protein consisting 222 amino acid residues with a calculated molecular mass of 25.1 kDa. The analysis by gel filtration chromatography...
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Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana
PublicationBACKGROUND: In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods.RESULTS: We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (TmaSSB) and Thermotoga neapolitana (TneSSB). They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino...
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Novel DNA-binding protein from Nanoarchaeum equitans Kin4-M binds all kinds of nucleic acids
PublicationNanoarchaeum equitans is the only known representative of Archaea phylum Nanoarchaeota and stands out as one of the tiniest known living organism. What is more it has smallest genome, which is only 490.885 base pairs long. It is also one of the most compact genomes. According to predictions about 95% of the DNA encodes proteins or stable RNA. Nanoarchaeum equitans lacks genes for most vital metabolic pathways including lipid, cofactor,...
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Defining a novel domain that provides an essential contribution to site-specific interaction of Rep protein with DNA
PublicationAn essential feature of replication initiation proteins is their ability to bind to DNA. In this work, we describe a new domain that contributes to a replication initiator sequence-specific interaction with DNA. Applying biochemical assays and structure prediction methods coupled with DNA–protein crosslinking, mass spectrometry, and construction and analysis of mutant proteins, we identified that the replication initiator of the...
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Application of stereology for two-phase flow structure validation in fluidized bed reactors
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Towards bees detection on images: study of different color models for neural networks
PublicationThis paper presents an approach to bee detection in videostreams using a neural network classifier. We describe the motivationfor our research and the methodology of data acquisition. The maincontribution to this work is a comparison of different color models usedas an input format for a feedforward convolutional architecture appliedto bee detection. The detection process has is based on a neural...
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Experimental Investigation of an Intensified Heat Transfer Adsorption Bed (IHTAB) Reactor Prototype
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Performance Evaluation of a Single-Stage Two-Bed Adsorption Chiller With Desalination Function
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Stevia, kaa hee, wild sweet herb from South America - An overview
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Przykłady napraw zbiorników ropy naftowej bez wyłączania ich z eksploatacji
PublicationOmówiono dwa przykłady prac remontowych prowadzonych na zbiornikach wypełnionych ropą naftową. Remonty dotyczyły:1)korekty kształtu płaszcza zbiornika2)przedłużenia króćców włazów w komorach dachu pływającego.Omowiono technologię naprawy oraz zastosowane zabezpieczenia przed wybuchem.
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Nowa metoda pomiaru impedancji pętli zwarciowej bez wyzwalania wyłączników różnicowoprądowych
PublicationW referacie przedstawiono problematykę pomiaru impedancji pętli zwarciowej w sieciach z wyłącznikami różnicowoprądowymi (RCD - Residul Current Device) oraz zaproponowano nową metodę i układ do pomiaru impedancji pętli bez wyzwalania wyłączników RCD. Nowa metoda umożliwia przeprowadzenie pomiaru przy wykorzystaniu dużej wartości krótkotrwałego całofalowego prądu pomiarowego, co wpływa korzystnie na dokładność pomiaru. Metoda ta...
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Napęd elektryczny z silnikiem klatkowym pracujący bez pomiaru prędkości obrotowej.
PublicationW artykule przedstawiono układ bezczujnikowego sterowania silnikiem asynchronicznym klatkowym. Koncepcja układu sterowania wykorzystuje metodę nieliniowego sterowania silnikiem asynchronicznym, która zapewnia odsprzężenie torów regulacji momentu i strumienia silnika. Do wyznaczania zmiennych układu regulacji takich jak strumień wirnika i prędkość kątowa zastosowano obserwatory wykorzystujące zależności modelu silnika asynchronicznego.
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Sprawność układów turboparowych z przegrzewem wewnętrznym i bez przegrzewu wewnętrznego.
PublicationW pracy przedstawiono porównanie sprawności ukłądów turboparowych z i bez wewnętrznego przegrzewu pary. W oparciu o analizę stwierdzono, że układ z przegrzewem ma wyższą sprawność niż bez przegrzewu.
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FLUID BED COATING OF MINITABLETS AND PELLETS WITH OPTIMIZATION OF THE PROCESS BASED ON TAGUCHI METHOD
PublicationSmall particles like pellets are coated in fluid bed systems. This method can be also feasible for minitablets but the selection of optimal process parameters is complicated. The aim of the research was to optimize the coating process for minitablets and to compare the conditions required for pellets. Minimum fluidization velocities (umf) for 2.0 and 2.5 mm minitablets and 0.7-0.8 mm or 1.0-1.25 mm pellets were determined experimentally....
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One-step recovery of REE oxalates in electro-leaching of spent NdFeB magnets
PublicationRecovery of rare-earth elements (REEs) from spent NdFeB magnets is receiving great attention because of high amount of neodymium and potential risk of environmental pollution. In this study, a novel environment-friendly hydrometallurgical route is proposed for efficient recovery of REEs during electrochemical leaching with sulfuric and oxalic acids. With proper adjustment of the electrolyte composition and operating conditions,...
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Sugar Fee in Poland as an Example of New Fiscal Burdens and Stimulation of Consumer Behaviour
PublicationDuring the economic downturn caused by the COVID-19 pandemic, the amount and structure of tax burdens imposed by the state on businesses continue to increase, as shown on the example of the newly introduced sugar fee. A new tax-related burden imposed on businesses and consumers producing and consuming sugar-sweetened beverages appeared at the beginning of 2021. The aim of the paper is to present the fiscal burdens that accompany...
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Structure and magnetic properties of BeO-Fe2O3-Al2O3-TeO2 glass-ceramic composites
PublicationIn this work, glass-ceramics in the xBeO–20Fe2O3–(80-x)TeO2 system with x = 0–25 mol% were synthesized by the traditional melt quenching route and studied by inductively coupled plasma optical emission spectroscopy, X-ray diffraction, confocal microscopy, infrared and Raman spectroscopy. BeO addition was found to support the crystallization process of Fe2O3 during melting, and an increased BeO content was associated with an increased...
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Assessment of diversity and composition of bacterial community in Sludge Treatment Reed Bed systems
PublicationDue to their low emission of odours and lack of the need to apply additional chemical agents, sludge treatment reed beds (STRBs) constitute an economically feasible and eco-friendly approach to sewage sludge management. Correctly designed and operated STRBs ensure effective reduction of the dry matter content coupled with the mineralisation of organic compounds. Successful operation of STRBs relies on complex interactions between...
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Badanie wykonalności pomiaru impedancji pętli zwarciowej bez wyzwalania wyłączników różnicowoprądowych
PublicationRozprawa doktorska dotyczy rozwiązania problemu, jakim jest wyzwalanie wyłączników różnicowoprądowych podczas pomiaru impedancji (rezystancji) pętli zwarciowej w obwodach, które są przez te wyłączniki chronione. Pomiar impedancji pętli zwarciowej metodą tradycyjną wykorzystującą sztuczne zwarcie, szczegółowo opisaną w normie PN HD 60364 6:2008, jest skuteczny jedynie w obwodach, które nie są chronione wyłącznikami różnicowoprądowymi....
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Possible effects of the 1984 St. Clair River ice jam on bed changes
PublicationThis study examines the possible effect of the record ice jam of 1984 in the St. Clair River on river bed changes and conveyance. Numerical simulations were made to examine the flow and bed shear stresses during the jam formation and release periods. Simulation results indicate that the ice jam in the river did not cause a significant increase in bed shear stress compared to pre- and post-jam open water conditions. The insignificant...
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The application of moving bed biofilm reactor to denitrification process after trickling filters
PublicationThe paper presents research of a prototype moving bed biofilm reactor (MBBR). The device was used for the post-denitrification process and was installed at the end of a technological system consisting of a septic tank and two trickling filters. The concentrations of suspended biomass and biomass attached on the EvU Perl moving bed surface were determined. The impact of the external organic carbon concentration on the denitrification...
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Properties of water in the region between a tubulin dimer and a single motor head of kinesin
PublicationA kinesin is a molecular motor that can perform movement on a microtubule track in a stepping-like manner. This motion is connected with processes of association and dissociation of kinesin and tubulin. Water is an important participant in these kinds of molecular interactions. This is why we have decided to investigate the dynamical and structural properties of water in the region between the kinesin catalytic domain and the tubulin...
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Proprotein Convertase Subtilisin/Kexin Type 9, Angiopoietin-Like Protein 8, Sortilin, and Cholesteryl Ester Transfer Protein—Friends of Foes for Psoriatic Patients at the Risk of Developing Cardiometabolic Syndrome?
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Patterned protein microarrays for bacterial detection. Langmuir**2003 vol.19 s. 436-439, 5 rys. bibliogr. 25 poz. Specyficzne mikroukłady protein do wykrywania bakterii.
PublicationSkonstruowano i przetestowano specyficzne mikroukłady przeciwciał selektyw-nie wiążących bakterie. Otrzymane układy umożliwiają wykrywanie bakterii E.coli0157:H7 oraz Renibacterium salmoninarum (RS). Matryce wytworzono techni-ką nadruku mikrokontaktów (ćCP). Charakterystykę układów uzyskano stosującmikroskopię skaningową (SPM). Ustalono, że selektywność otrzymanych układówobniża się podczas ekspozycji na bakterie niespecyficzne.
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Investigation of interaction between xanthene dye rhodamine B and metagenomic-derivated methylthioadenosine phosphorylase RSFP
PublicationIn the previous study we found that the expression of the metagenomic - derived methylthioadenosine phosphorylase gene (rsfp) in E. coli cells and the presence of rhodamine B in the growth medium were crucial for the pink fluorescence of one E. coli colony from Antarctic soil metagenomic library (Cieśliński et al., 2009). In this study, rsfp gene expression was induced by an arabinose promoter in an LMG194 E. coli strain and the...
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Mycobacterium tuberculosis Complex and Mycobacterial Heat Shock Proteins in Lymph Node Tissue from Patients with Pulmonary Sarcoidosis
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Effect of Inoculation with Probiotics and Ageing Time on Selected Functional Properties and Oxidation of Proteins in Dry-Cured Pork Loins
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