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Search results for: IN VIVO EXPERIMENTATION
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Application of Mathematics in Technology, ET, 24/25
e-Learning CoursesApplication of Mathematics MOODLE ENV localdevtestprod
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Communication and Visualisation in Building Automation - 2024/25
e-Learning CoursesLecture "Communication and Visualisation in Building Automation", Wydział ETI, katedra KSiS, II stopień studiów
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Communication and Visualisation in Building Automation - 2023/24
e-Learning CoursesLecture "Communication and Visualisation in Building Automation", Wydział ETI, katedra KSiS, II stopień studiów
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[AiU] Research methodology in architecture and urban planning
e-Learning CoursesW ramach kursu realizowane będą zajęcia dla grupy doktorantów szkoły doktorskiej z dyscypliny "Architektura i urbanistyka". This course is addressed to the PhD students of "Architecture and urbanism" discipline at the doctoral school. Course type: lecture Total hours of training: 30 teaching hours
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Application of Mathematics in Technology 2 [2023/24]
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Forecasting and optimalization in logistics (Winter 24/25)
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CONTROL SYSTEMS IN RENEWABLE ENERGY SOURCES 2023/2024
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Identification and properties of the Deinococcus grandis andDeinococcus proteolyticus single-stranded DNA binding proteins (SSB)
PublicationW celu poznania właściwości biochemicznych białek SSB pochodzących z Deinococcus grandis (DgrSSB) i Deinococcus proteolyticus (DprSSB) sklonowano zaamplifikowane w reakcji PCR geny ssb w systemie ekspresyjnym Escherichia coli. Geny składają się z 891(DgrSSB) i 876 (DprSSB) nukleotydów kodujących odpowiednio 296 i 291 reszt aminokwasowych o teoretycznie wyznaczonej masie cząsteczkowej dla białek, równej 32,29 i 31,33 kDa. Sekwencja...
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Novel thermostable single-stranded DNA-binding protein (SSB) from Deinococcus geothermalis
PublicationW celu zbadania właściwości biochemicznych białka wiążącego się do jednoniciowego DNA (SSB) pochodzącego z Deinococcus geothermalis (DgeSSB), sklonowano gen ssb otrzymany w reakcji PCR, a następnie uzyskano wydajną biosyntezę białka DgeSSB. Gen składa się z 900 par zasad, kodujących białko złożone z 300 reszt aminokwasowych, o wyliczonej masie molekularnej równej 32.45 kDa. Sekwencja aminokwasowa wykazuje 43, 44% i 75% identyczności...
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Bioactive and bioavailability of minerals in rats loaded with cholesterol and kiwi fruit
PublicationThe aim of this study was to compare the content of polyphenols (TP), minerals, ascorbic acid (AA) and total antioxidant capacities (TAC) of conventional and organic kiwifruit ‘Hayward’ treated with ethylene after harvest and to determine their influence on plasma TAC, mineral content in the liver and bioavailability (RBV — relative bioavailability value) in rats fed diet containing cholesterol. Organic and conventionally grown...
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Successive cytotoxicity control by evolutionary surface decorated electronic push-pull green ZnCr-LDH nanostructures: Drug delivery enlargement for targeted breast cancer chemotherapy
PublicationThe reason for the increasing bioavailability and biocompatibility of the porous nanomaterials in the presence of different (bio)molecules is still unknown. The role of difference functional groups and their interactions with the potential bioavailability and biocompatibility is of great importance. To investigate the potential contribution of the electronic effects (especially on the surface of the porous nanomaterials) on their...
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Iron–Sulfur Cluster Biogenesis Chaperones: Evidence for Emergence of Mutational Robustness of a Highly Specific Protein–Protein Interaction
PublicationBiogenesis of iron–sulfur clusters (FeS) is a highly conserved process involving Hsp70 and J-protein chaperones. However, Hsp70 specialization differs among species. In most eukaryotes, including Schizosaccharomyces pombe, FeS biogenesis involves interaction between the J-protein Jac1 and the multifunctional Hsp70 Ssc1. But, in Saccharomyces cerevisiae and closely related species, Jac1 interacts with the specialized Hsp70 Ssq1,...
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IN VITRO PROPAGATION OF RHODODENDRON TOMENTOSUM - AN ENDANGERED ESSENTIAL OIL BEARING PLANT FROM PEATLAND
PublicationRhododendron tomentosum Harmaja (formerly Ledum palustre L.) is a medicinal peat bog plant native to northern Europe, Asia and North America. This plant has a distinctive aroma thanks to the presence of essential oil, to which it also owes its anti-inflammatory, analgesic, antimicrobial and insecticidal properties. However, in Europe R. tomentosum is classified as an endangered species, mainly due to degradation of peatlands. In...
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Synthesis, Chemical Characterization and Multiscale Biological Evaluation of a dimericcRGD Peptide for Targeted Imaging of αVβ3 Integrin Activity
PublicationCyclic peptides containing the Arg-Gly-Asp (RGD) sequence have been shown to specifically bind the angiogenesis biomarker α V β3 integrin. We report the synthesis, chemical characterization, and biological evaluation of two novel dimeric cyclic RGD-based molecular probes for the targeted imaging of α V β3 activity (a radiolabeled version, 64Cu-NOTA-PEG4-cRGD2, for PET imaging, and a fluorescent version, FITC-PEG4-cRGD2, for in...
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Anomalous diffusion modeling using ultracapacitors in domino ladder circuit
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Prediction of Stress and Deformation Caused by Magnetic Attraction Force in Modulation Elements in a Magnetically Geared Machine Using Subdomain Modeling
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Essentials of computer science for Bachelor in Management students
e-Learning CoursesVarious resources accompanying "Essentials of computer science" classes for Bachelor in Management 1st year students.
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[ITiT] Transmission Channel in Radio Communication Systems - 2023/2024
e-Learning Courses{mlang pl} Dyscyplina: informatyka techniczna i telekomunikacja Zajęcia fakultatywne dla doktorantów II roku Prowadzący: dr hab. inż. Sławomir Ambroziak, prof. PG Liczba godzin: 15 Forma zajęć: wykład {mlang} {mlang en} Discipline: technical informatics and telecommunications Elective course for 2nd-year PhD students Academic teachers: dr hab. inż. Sławomir Ambroziak, prof. PG Total hours of training: 15 teaching hours Course...
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[soft skills] Didactic methods in the modern teaching process
e-Learning Courses{mlang pl} Dyscyplina: wszystkie dyscypliny Zajęcia obowiązkowe dla doktorantów II roku Prowadzący: dr hab. Joanna Mytnik, prof. PG, mgr Alina Guzik, Centrum Nowoczesnej Edukacji Liczba godzin: 15 Forma zajęć: wykład Kurs realizowany w zakładce Centrum Nowoczesnej Edukacji: https://enauczanie.pg.edu.pl/moodle/course/view.php?id=10875 Na kurs należy zapisać się samodzielnie: https://enauczanie.pg.edu.pl/moodle/enrol/index.php?id=10875 Hasło...
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[NCh-bio] Advances in Biotechnology: Protein Folding and Assembly
e-Learning Courses{mlang pl} Dyscyplina: nauki chemiczne Zajęcia obowiązkowe dla doktorantów I i II roku z obszaru biotechnologii Prowadzący: dr hab. Gracjana Klein-Raina, prof. PG Liczba godzin: 15 Forma zajęć: wykład {mlang} {mlang en} Discipline: chemical sciences Obligatory course for 1st and 2nd-year PhD students from biotechnology area Academic teachers: dr hab. Gracjana Klein-Raina, prof. PG Total hours of training: 15 teaching...
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Application of Mathematics in Technology for Dezhou University, 2022
e-Learning CoursesApplication of Mathematics
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[Soft Skills] Didactic methods in the modern teaching process
e-Learning Courses{mlang pl} Dyscyplina: wszystkie dyscypliny Zajęcia obowiązkowe dla doktorantów II roku ProwadzącA: mgr Olga Ludyga Liczba godzin: 15 Forma zajęć: warsztaty {mlang} {mlang en} Discipline: all disciplines Obligatory course for 1st-year PhD students Academic teacher: mgr Olga Ludyga Total hours of training: 15 teaching hours Course type: workshop {mlang} Soft skills, area II - didactic skills
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Communication and Visualisation in Building Automation - 2022/23 - Nowy
e-Learning CoursesLecture "Communication and Visualisation in Building Automation", Wydział ETI, katedra KSA, II stopień studiów
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New and Fast Analytical Techniques in Food Quality Analysis
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Placement Test 2nd Degree (Studies in English) 1 sem
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Advances in materials engineering instrumentation: new trends and applications
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Contemporary directions of development in mechanical engineering kopia 1
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Architectural and Urban Theories in Revitalization Process 2023/24
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Numerical Modelling in flow systems design_W/S_Energetyka_sem6_lato 23/24_PG_00042087
e-Learning CoursesPrzedstawienie podstaw modelowania komputerowego procesów mających zastosowanie w technice cieplnej tak aby słuchacz był w stanie zrozumieć i zinterpretować wyniki otrzymane przy pomocy kodów obliczeniowych
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Polymeric materials in medicine – structure and desing 2023/24
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Arch. & Urban Theories in Revitalisation Process 2024/25
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[AiU]20232024_Challenges and Perspectives in Contemporary Architecture and Urbanism
e-Learning CoursesThis course is compulsory for PhD students assigned to Architecture and Urbanism tracks at Doctoral School at Gdańsk University of Technology. The course is conducted by Prof. Marichela Sepe, DICEA / Sapienza University Rome Course type: workshops Total hours of training: 15 teaching hours Classes in online mode (classes conducted online)
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Topology and Dynamical Systems in Action - Summer school 2023
e-Learning CoursesThe course is designed for participants in the Spinnaker summer school in mathematics.
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Application of Mathematics in Technology for Dezhou University, 2024
e-Learning CoursesApplication of Mathematics MOODLE ENV localdevtestprod
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Space and Landscape in a Global Context - 2023/24
e-Learning CoursesModule SPACE AND LANDSCAPE IN A GLOBAL CONTEXT (2022/2023, autumn semester) Objective The objective of the module is to debate different approaches to managing spatial development and contemporary issues in a global context. The course is founded on the students' knowledge about urban planning and instruments used to make them better places both for people and the environment. Course proceedings Lectures and classes run online...
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Application of Mathematics in Technology for Dezhou University, 2023
e-Learning CoursesApplication of Mathematics MOODLE ENV localdevtestprod
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[Soft Skills] Workshop in research ethics gr.1 AIU_ILGIT_IŚGIE_EIF
e-Learning Courses{mlang pl} Dyscyplina: Wszystkie dyscypliny Zajęcia obowiązkowe dla doktorantów III roku Prowadzący: A. Karalus Liczba godzin: 5 {mlang} {mlang en} Discipline: All disciplines Obligatory course for 3rd year PhD students Academic teacher: A. Karalus Total hours of training: 5 teaching hours {mlang}
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Microbe Power and Diversity: in Health and Society - wykład
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Badanie możliwości określania parametrów ruchu statku za pomocą systemu EGNOS
PublicationW niniejszym artykule zaprezentowano badania dokładności względnej wyznaczeń współrzędnych pozycji za pomocą trzech jednorodnych odbiorników systemu EGNOS. Ich celem było sprawdzenie możliwości wykorzystania systemu do określania parametrów ruchu statku, w tym kątów orientacji przestrzennej kadłuba. Opisano podstawy teoretyczne, przebieg eksperymentu oraz wyniki analiz zarejestrowanych danych. Badania przeprowadzono na morzu latem...
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Focused ion beam-based microfabrication of boron-doped diamond single-crystal tip cantilevers for electrical and mechanical scanning probe microscopy
PublicationIn this paper, the fabrication process and electromechanical properties of novel atomic force microscopy probes utilising single-crystal boron-doped diamond are presented. The developed probes integrate scanning tips made of chemical vapour deposition-grown, freestanding diamond foil. The fabrication procedure was performed using nanomanipulation techniques combined with scanning electron microscopy and focused ion beam technologies....
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Honey antibacterial activity: A neglected aspect of honey quality assurance as functional food
PublicationBackground Honey is considered as a functional food with health-promoting properties. Its potent antibacterial and antibiofilm effects are the major attributes of so called ‘medical-grade honey’ which is topically used for the treatment of burns, wounds and skin disorders. Nevertheless, the current set of honey quality parameters adopted in the European Union do not include its biological properties. Furthermore, in light of the...
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Determination of estrogenic endocrine disruptors in environmental samples - a review of chromatographic methods
PublicationWśród związków endokrynnych to estrogeny zarówno naturalne jak i syntetyczne zostały wskazane i opisane jako związki niosące ze sobą największy potencjał aktywności estrogennej. Wysoki potencjał estrogenny jaki posiadają związki z grupy hormonów płciowych potwierdziły badania przeprowadzone zarówno in vitro jak i in vivo. W dużej części (50-95%) próbek ścieków pochodzących z oczyszczalni ścieków zostały wykryte związki z grupy...
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Teloxantron inhibits the processivity of telomerase with preferential DNA damage on telomeres
PublicationTelomerase reactivation is one of the hallmarks of cancer, which plays an important role in cellular immortalization and the development and progression of the tumor. Chemical telomerase inhibitors have been shown to trigger replicative senescence and apoptotic cell death both in vitro and in vivo. Due to its upregulation in various cancers, telomerase is considered a potential target in cancer therapy. In this study, we identified...
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Molecular basis for the DNA damage induction and anticancer activity of asymmetrically substituted anthrapyridazone PDZ-7
Publicationnthrapyridazones, imino analogues of anthraquinone, constitute a family of compounds with remarkable anti-cancer activity. To date, over 20 derivatives were studied, of which most displayed nanomolar cytotoxicity towards broad spectrum of cancer cells, including breast, prostate and leukemic ones. BS-154, the most potent derivative, had IC50 values close to 1 nM, however, it was toxic in animal studies. Here, we characterize another...
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A pilot study with flow mediated skin fluorescence: A novel device to assess microvascular endothelial function in coronary artery disease
PublicationBackground: Endothelial dysfunction is one of the earliest vascular manifestations in the pathogenesis of cardiovascular disease. Noninvasive, simple and inexpensive methods of endothelial function assessment are therefore needed. Methods: Microvascular endothelial function was assessed in coronary artery disease (CAD) patients by flow mediated skin fluorescence (FMSF), based on measurements of NADH fluorescence intensity during...
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A standardised static in vitro digestion method suitable for food – an international consensus
PublicationSimulated gastro-intestinal digestion is widely employed in many fields of food and nutritional sciences, as conducting human trials are often costly, resource intensive, and ethically disputable. As a consequence, in vitro alternatives that determine endpoints such as the bioaccessibility of nutrients and non-nutrients or the digestibility of macronutrients (e.g. lipids, proteins and carbohydrates) are used for screening and building...
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Antibiotic-Based Conjugates Containing Antimicrobial HLopt2 Peptide: Design, Synthesis, Antimicrobial and Cytotoxic Activities
PublicationRecent studies have shown that modified human lactoferrin 20−31 fragment, named HLopt2, possesses antibacterial and antifungal activity. Thus, we decided to synthesize and evaluate the biological activity of a series of conjugates based on this peptide and one of the antimicrobials with proven antibacterial (ciprofloxacin, CIP, and levofloxacin, LVX) or antifungal (fluconazole, FLC) activity. The drugs were covalently connected...
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Generation and Characterization of a DNA-GCN4 Oligonucleotide-Peptide Conjugate: The Impact DNA/Protein Interactions on the Sensitization of DNA
PublicationRadiotherapy, the most common therapy for the treatment of solid tumors, exerts its effects by inducing DNA damage. To fully understand the extent and nature of this damage, DNA models that mimic the in vivo situation should be utilized. In a cellular context, genomic DNA constantly interacts with proteins and these interactions could influence both the primary radical processes (triggered by ionizing radiation) and secondary reactions,...
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Instrument detection and pose estimation with rigid part mixtures model in video-assisted surgeries
PublicationLocalizing instrument parts in video-assisted surgeries is an attractive and open computer vision problem. A working algorithm would immediately find applications in computer-aided interventions in the operating theater. Knowing the location of tool parts could help virtually augment visual faculty of surgeons, assess skills of novice surgeons, and increase autonomy of surgical robots. A surgical tool varies in appearance due to...
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Webinarium „Drug Discovery, Development & Commercialization”
EventsCTWT PG zaprasza na webinarium „Drug Discovery, Development & Commercialization”. Spotkanie skierowane jest do osób zajmujących się zawodowo lub planujących swoją karierę w dziedzinie opracowywania i rozwoju leków.