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Estimation of electrode contact in capacitive ECG measurement
PublikacjaIn the paper a method of electrode’s contact estimation in capacitive electrocardiogram (CECG) is presented. Proposed solution allows estimation of contact quality for each individual electrode. This enables construction of multi-electrode CECG systems, where electrode pairs can be selected on the basis of the individual electrode contact quality.
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Diagnostic PCR assay for Microsporum and Trichophyton infections
PublikacjaWe have previously described a highly sensitive 5-hour PCR test for the rapid diagnosis of onychomycosis which detects any dermatophyte and species-identify T. rubrum from patient specimens. We have subsequently developed and evaluated new PCR tests for detection of Trichophyton and Microsporum canis/audouinii infections.58 dermatophyte isolates (21) Microsporum spp. (4 different species), 35 Trichophyton spp (10 different species)...
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A 3-dB coupler with UC-PBG structure
PublikacjaPrzedstawiono nową konstrukcję sprzęgacza z liniami paskowymi sprzężonymi szerokim bokiem oraz zmodyfikowaną warstwą metalizacji masy. Modyfikacja metalizacji polega na periodycznym wytrawieniu dwuwymiarowej struktury szczelin, nazywanej strukturą uniplanarnej zwartej przerwy fotonicznej (UC-PBG: Uniplanar Compact Photonic BandGap). Potwierdzono przewidywane wcześniej zjawisko spowolnienia fali oraz powiększenia wielkości sprzężenia...
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Ligation Mediated PCR methods, their possibilities and limitations
PublikacjaThe application of molecular diagnostics for bacterial strain typing or identifying bacteria at the strain level is of particular importance at present. However, among the many genotyping methods that are currently available, no single one is universally ideal. In this article, we thus review the Ligation Mediated PCR group methods as prospects from which to choose. We discuss different strategies for selecting the amplified PCR...
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Optimal ECG lead for deriving respiratory signal
PublikacjaEDR is an interesting measuring technique that allows an indirect assessment of respiratory activity. This is an alternative solution to direct methods that are based on the measurement of air flow, which require a specialized sensor or even a system. However, due to inter-personal anatomical differences, the optimal ECG lead (placement of the electrodes) ensuring the best EDR signal quality is not fixed. An influence of ECG lead...
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PCR-Based Genotyping of Mycobacterium tuberculosis with New GC-Rich Repeated Sequences and IS6110 Inverted Repeats Used as Primers.
PublikacjaW pracy przedstawiono nową metodę genotypowania Mycobacterium tuberculosis. Zastosowanie oligonukleotydu Mtb1 5'-CCG-GCG-GGG-CCG-GCG-G lub Mtb2 5'-CGG-CGG-CAA-CGG-CGG-C wraz z oligonukleotydami specyficznymi do terminalnych sekwencji elementu insercyjnego IS6110 pozwala na różnicowanie szczepów Mycobacterium tuberculosis w metodzie PCR. Opracowana metoda może być wykorzystana w badaniach epidemiologicznych gruźlicy.
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Solar-Terrestrial Physics
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Nursing older people
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Working with Older People
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SOLAR SYSTEM RESEARCH
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The New LM-PCR/Shifter Method for the Genotyping of Microorganisms Based on the Use of a Class IIS Restriction Enzyme and Ligation Mediated PCR
PublikacjaThis study details and examines a novel Ligation-Mediated - Polymerase Chain Reaction (LM-PCR) method. Named the LM-PCR/Shifter, it relies on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base, 5' overhangs, this being the Shifter, and the ligation of appropriate oligonucleotide adapters. A sequence of 4-base, 5' overhangs of the adapter and a 4-base sequence of the 3' end of the primer(s)...
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Removal of sulfur from a solvent extract
PublikacjaThe presence of sulfur in a solvent extract that is to be analyzed chromatographically impairs and sometimes precludes properinterpretation of the chromatogram, as the sulfur peak masks the peaks of other compounds present in the sample, and sulfur alsodisrupts the operation of the mass detector. This means that discrepancies in the results can lead to erroneous interpretations andfalse assessments of environmental risk. For this...
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Silver filled single-wall carbon nanotubes—synthesis, structural and electronic properties
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Influence of Preparation Procedure on Physicochemical and Antibacterial Properties of Titanate Nanotubes Modified with Silver
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Modifications of Hydroxyapatite by Gallium and Silver Ions—Physicochemical Characterization, Cytotoxicity and Antibacterial Evaluation
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Lattice dynamics and elasticity of silver thiogallate (AgGaS2) from ab initio calculations
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Photostability pf Cy3 and Cy5-labeled DNA in the presence of metallic silver particles
PublikacjaZbadano wpływ cząstek metalicznego srebra na fotostabilność oligomerów DNA kowalencyjnie związanych z Cy3 lub Cy5. Zbliżenie fluoroforów do cząstek srebra powodowało zwiększenie intensywności fluorescencji i pojawienie się frakcji emisji bardziej odpornej na wybielanie.
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Silver-doped TiO2 prepared by microemulsion method: surface properties, bio- and photoactivity
PublikacjaOtrzymane fotokatalizatory typu Ag-TiO2 wykazywały wyższą aktywność w reakcjach fotodegradacji fenolu w obecności promieniowania z zakresu UV oraz światła widzialnego w porównaniu z komercyjnym katalizatorem Degussa P25 oraz czystym niedomieszkowanym TiO2, otrzymanym w mikroemulsji woda-AOT-cykloheksan. Powierzchnia właściwa (BET) otrzymanych katalizatorów wynosiła od 100 do 160 m2/g. Na podstawie analizy rentgenowskiej (XRD) stwierdzono,...
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Sub-lethal photodynamic inactivation renders Staphylococcus aureus susceptible to silver nanoparticles
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Electrochemical stability of TiO2 nanotubes deposited with silver and gold nanoparticles in aqueous environment
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Influence of silver nanoparticles addition on antibacterial properties of PEO coatings formed on magnesium
PublikacjaMagnesium is a biodegradable material and thus could be a choice for bone fixation devices and implants with a specific purpose. This study aims to enhance the anti-corrosive, biocompatible, and antibacterial properties on magnesium-based materials through ceramic coatings formation. To achieve this the silicate-based electrolyte was used to create of Plasma Electrolytic Oxidation (PEO) coatings. During investigation the bioactive...
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From phosphanylphosphaalkenes to coordination copper and silver polymers containing P–P bonds
PublikacjaThis study was focused on the activation of the C[double bond, length as m-dash]P bond via reactions of Ph2C[double bond, length as m-dash]P-PtBu2 (1) with 1,6-hexanediol and selected dithiols (1,4-butanedithiol, 1,4-benzenedithiol and 1,4-benzenedimethanethiol). These reactions proceed according to a 1,2-addition mechanism, providing new compounds with formulas {(Ph)2(H)C-P-PtBu2}{μ2-(O-(CH2)6-O)}{tBu2P-P-C(H)(Ph)2} (2), {(Ph)2(H)C-P-PtBu2}{μ2-(S-(CH2)4-S)}{tBu2P-P-C(H)(Ph)2}...
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Electrochemical activity of composite material poly(3,4-ethylenedioxythiophene) modified by silver hexacyanoferrate
PublikacjaW pracy przedstawiono sposób slektrochemicznj syntezy materiału składającego się z polimeru przewodzącego poli(3,4-etylenodioksytiofen) (pEDOT) i nieorganicznej sieci typu redox heksacyjanożelazianu srebra (Aghcf). Materiał otrzymano na drodze dwuetapowej. Etap pierwszy polega na galwanostatycznej redukcji srebra na elektrodzie z węgla szklistego. Etap drugi to roztwarzanie srebra z jednoczesną polimeryzacją monomeru. Otrzymany...
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Chapter 31. Preparation of silver doped titanium dioxide nanoparticles in microemulsion system
PublikacjaNanocząstki TiO2 domieszkowanego srebrem przygotowano w mikroemulsji woda-AOT-cykloheksan. Zbadano wpływ zawartości srebra (od 1,5 do 8,5% mol.) na aktywność fotokatalityczą oraz właściwości bakterio- i grzybobójcze. Stwierdzono, że TiO2 modyfikowany za 6,5% mol. Ag wykazuje najwyższą aktywność w reakcji degradacji fenolu oraz najwyższą aktywność mikrobiologiczną. Aktywność mikrobiologiczną wyznaczano jako minimalne stężenie hamujące...
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Silver Nanoparticles Combined With Naphthoquinones as an Effective Synergistic Strategy Against Staphylococcus aureus
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Properties of ultrafine-grained Mg-based composites modified by addition of silver and hydroxyapatite
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Optical properties of a dielectric slab front-loaded with a grating of plasmonic silver nanowires
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“Silver Economy” Models in the European Union in the Comparative Approach: An Attempt to Introduce Discussion
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Potentiometric and AM1d studies of a new class of Tr-podands–silver(I) complexes
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GPU Acceleration of Multilevel Solvers for Analysis of Microwave Components With Finite Element Method
PublikacjaThe letter discusses a fast implementation of the conjugate gradient iterative method with ${rm E}$-field multilevel preconditioner applied to solving real symmetric and sparse systems obtained with vector finite element method. In order to accelerate computations, a graphics processing unit (GPU) was used and significant speed-up (2.61 fold) was achieved comparing to a central processing unit (CPU) based approach. These results...
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Silver complexes stabilized by large silanethiolate ligands – crystal structures and luminescence properties
PublikacjaBulky silanethiolate and disiladithiolate ligands were applied to synthesize one mononuclear and three trinuclear silver complexes including two cyclic “microclusters” and a linear tri-nuclear silanethiolate complex. All obtained compounds are characterized by X-ray diffraction and FT-IR. NMR and emission spectroscopies were used where possible. The first trinuclear anionic silver thiolate is structurally characterized. The influence...
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Spontaneous electron emission vs dissociation in internally hot silver dimer anions
PublikacjaReferring to a recent experiment, we theoretically study the process of a two-channel decay of the diatomic silver anion (Ag2-), namely the spontaneous electron ejection giving Ag2 + e- and the dissociation leading to Ag- + Ag. The ground state potential energy curves of the silver molecules of diatomic neutral and negative ion were calculated using proper pseudo-potentials and atomic basis sets. We also estimated the non-adiabatic...
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A GPU Solver for Sparse Generalized Eigenvalue Problems with Symmetric Complex-Valued Matrices Obtained Using Higher-Order FEM
PublikacjaThe paper discusses a fast implementation of the stabilized locally optimal block preconditioned conjugate gradient (sLOBPCG) method, using a hierarchical multilevel preconditioner to solve nonHermitian sparse generalized eigenvalue problems with large symmetric complex-valued matrices obtained using the higher-order finite-element method (FEM), applied to the analysis of a microwave resonator. The resonant frequencies of the low-order...
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Electrochemical detection of bacterial endotoxin lipopolysaccharide (LPS) on gold electrode modified with DAL-PEG-DK5-PEG-OH - Antimicrobial peptide conjugate
PublikacjaThis work describes fabrication of gold electrodes modified with peptide conjugate DAL-PEG-DK5-PEG-OH that enables ultra-sensitive detection of lipopolysaccharide (LPS) isolated from the reference strain of Escherichia coli O26:B6. The initial step of the established procedure implies immobilization of the fully protected DAL-PEG-DK5-PEG-OH peptide on the surface of the gold electrode previously modified by cysteamine. Then side...
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MultiRegional PCA for leakage detection and localisation in DWDS - approach
PublikacjaMonitoring is one of the most important parts in advanced control of complex dynamic systems. Information about systems behavior, including failures indicating, enables for efficient control. The chapter describes an approach to detection and localisation of pipe leakage in Drinking Water Distribution Systems (DWDS) representing complex and distributed dynamic system of large scale. Proposed MultiRegional Principal Component Analysis...
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The New LM-PCR/Shifter Method for the Genotyping of Microorganism
PublikacjaTechniques relies on the ligation of appropriates adapters (LM-PCR) as AFLP, PCR MP and ADSRRS are successfully used for epidemiological studies for prokaryotic and eukaryotic microorganisms. In this study we propose a new method, called the LM-PCR/Shifter, based on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base 5' overhangs (Shifter) and the ligation of appropriate oligonucleotide...
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Real-Time PCR: molecular technique of many applications
PublikacjaReal-Time PCR is a sensitive DNA amplification technique initially applied in genetics and molecular biology. It enables in vivo copying of the selected DNA fragment (flanked by two primers) by the thermostable polymerase (in the presence of magnesium ions and deoxynucleotide triphosphates) and simultaneous measurement of the fluorescence. For one or more specific sequences in a DNA sample, real-time PCR enables both detection...
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DSC coupled with PCA as a tool for butter authenticity assessment
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Expression of hCG and GnRHs and Their Receptors in Endometrial Carcinoma and Hyperplasia
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Capacitively coupled ECG measurements - a CMRR circuit improvement
PublikacjaA typical galvanic-connected electrocardiogram (ECG) measurement system utilizes two signal’s electrodes and a third one in driven-right-leg (DRL) circuit for improving a common-mode rejection ratio(CMRR) of the acquisition system. In capacitive-coupled ECG similar techniques are used, however it is expected, that the utilized DRL subsystem is formed using a capacitive coupling approach, too. An improvement of the acquisition system...
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A scale with ECG measurements capability for home cardiac monitoring
PublikacjaPresented device -a scale developed for enabling extra measurements, in addition to body weight and composition, the electrical activity of the heart (electrocardiogram - ECG) and the level of oxygen saturation in the blood SaO2. Electrical activity is recorded in the form of six leads, socalled - limb leads. The device allows measurement of all standard limb leads and without the need for additional procedures. ECG, although the...
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PRZYDATNOŚĆ DIAGNOSTYCZNA REAKCJI PCR W ROZPOZNAWANIU BORELIOZY
PublikacjaBorelioza jest wieloukładową chorobą wywoływaną przez bakterie zaliczane do kompleksu Borrelia burgdorferi sensu lato. Wektorem przenoszącym infekcję są kleszcze z rodzaju Ixodes, które zakażają kolejnych żywicieli krętka podczas spożywania ich krwi. Zróżnicowany przebieg boreliozy uniemożliwia jej rozpoznanie na podstawie objawów klinicznych. W związku z tym diagnostyka choroby z Lyme opiera się głównie na metodach laboratoryjnych,...
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Over Multatuli
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Carnivorous plants used for green synthesis of silver nanoparticles with broad-spectrum antimicrobial activity
PublikacjaIn this study, we exploit the anti-oxidative potential of four carnivorous plants to produce uniform and biologically active silver nanoparticles. The use of polyvinylpyrrolidone promoted syn-thesis of quasi-spherical nanoparticles characterized by stability and high uniformity. Their activity was tested against three human pathogens and three species of plant pathogenic bacteria. The study demonstrates the influence of synthesis...
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Application of micellar electrokinetic chromatography for detection of silver nanoparticles released from wound dressing
PublikacjaThe recent emergence of nanotechnology has provided a new therapeutic modality in case of silver nanoparticles. Dressings containing silver form the basis for the treatment of burns and wounds, either acute or chronic ones. The aim of the study was to examine silver release from the different wound dressings: commercially available (Atrauman Ag, Aqua- cel Ag) and experimental (FKDP-AgNPs) using MEKC. In order to characterize prepared keratin...
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Drop-coating deposition surface-enhanced Raman spectroscopy on silver substrates for biofluid analysis
PublikacjaUtilization of surface-enhanced Raman spectroscopy as a measurement technique is of particular interest in biodetection due to its superb chemical specificity and high sensitivity. The use of SERS substrates further improve this method by massive enhancement of the molecule Raman spectrum, permitting very low levels of detection. Therefore it is important to seek for new ways to develop reliable substrates, which are quickly and...
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Effects of metallic silver particles on resonance energy transfer between fluorophores bound to DNA.
PublikacjaZbadano wpływ filmów wysepek metalicznego srebra na rezonansowe przekazywanie energii między fluoroforami związanymi z DNA. Donorem i akceptorem były odpowiednio 4´,6 diamidyno-2-fenylindon i jodek propydyny. Zmierzone dla tego układu czasy zaniku i widma fluorescencji pokazują, że sąsiedztwo cząstek srebra dramatycznie zwiększa promień Forstera, a tym samym i przekazywanie energii w znakowanym DNA.
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Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island film
PublikacjaOpisano fluorescencję barwnika CY5 połączonego z DNA w roztworze buforowym na wysepkach srebrowych.
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Electrodes Based on a Titanium Dioxide Nanotube–Spherical Silver Nanoparticle Composite for Sensing of Proteins
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photocatalytic activity of silver doped tio2 in vis and uv light, prepared by microemulsion method
PublikacjaDitlenek tytanu otrzymany w wyniku hydrolizy izopropanolanu tytanu oraz komercyjny katalizator TiO-6 (Catalysis Society of Japan, rutyl, wielkość cząstek 15 nm) zmodyfikowano srebrem metodą mikroemulsyjną. Aktywność fotokatalityczna otrzymanych nanokompozytów Ag-TiO2 została zbadana dla fotodegradacji fenolu pod działaniem promieniowania ultrafioletowego oraz widzialnego. Zaobserwowano, że na efektywność fotorozkładu modelowego...