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Search results for: IN VITRO LIPOLYSIS
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The psychrotrophic yeast Sporobolomyces roseus LOCK 1119 as a source of a highly active aspartic protease for the in vitro production of antioxidant peptides
PublicationA psychrotrophic yeast strain producing a cold-adapted protease at low temperature was classified as Sporobolomyces roseus. In standard YPG medium, S. roseus LOCK 1119 synthesized an extracellular protease with an activity of approximately 560 U/L. Optimization of medium composition and process temperature considerably enhanced enzyme biosynthesis; an approximate 70% increase in activity (2060 U/L). The native enzyme was purified...
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Photodynamic Inactivation of Bacteria with Porphyrin Derivatives: Effect of Charge, Lipophilicity, ROS Generation, and Cellular Uptake on Their Biological Activity In Vitro
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Field Cultivation and in vitro Cultures, Root-Forming Callus Cultures and Adventitious Root Cultures, of Panax quinquefolium as a Source of Ginsenosides
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Expression of high affinity choline transporter during mouse development in vivo and its upregulation by NGF and BMP-4 in vitro
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In Vitro Efficacy of Bacterial Cellulose Dressings Chemisorbed with Antiseptics against Biofilm Formed by Pathogens Isolated from Chronic Wounds
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Potential DPP IV Inhibitory Peptides from Dry-Cured Pork Loins after Hydrolysis: An In Vitro and In Silico Study
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In Vitro Antagonistic Effect of Gut Bacteriota Isolated from Indigenous Honey Bees and Essential Oils against Paenibacillus Larvae
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Antioxidant properties and bioactive constituents of some rare exotic thai fruits and comparison with conventional fruits. in vitro and in vivo studies
PublicationPrzedmiotem badań były ekstrakty uzyskane zarówno z egzotycznych owoców z terenu Tajlandii (durian, snake fruit, mangosteen) jak i owoców ''konwencjonalnych'' (mango, kiwi, awokado) w celu porównania zawartości związków bioaktywnych i aktywności przeciwutleniającej.Wyniki badań prowadzonych zarówno in vitro jak i in vivo mogą być wykorzystane przy opracowywaniu diety. W trakcie badań in vitro wykorzystano testy do określania potencjału...
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Towards plant-mediated chemistry – Au nanoparticles obtained using aqueous extract of Rosa damascena and their biological activity in vitro
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The Effect of Water-Soluble Polysaccharide from Jackfruit (Artocarpus heterophyllus Lam.) on Human Colon Carcinoma Cells Cultured In Vitro
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Human Adipose-Derived and Amniotic Fluid-Derived Stem Cells: A Preliminary In Vitro Study Comparing Myogenic Differentiation Capability
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In vitro studies of antimicrobial activity of Gly-His-Lys conjugates as potential and promising candidates for therapeutics in skin and tissue infections
PublicationWe presented in vitro studies of antimicrobial activity of Gly-His-Lys conjugates that are important point in preliminary biological evaluation of their potential application in skin and tissue therapy. The novel compounds include the conjugation of fatty acids with a modification of the amino acid sequence in the primary structure of Gly-His-Lys.
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Novel Nystatin A1 derivatives exhibiting low host cell toxicity and antifungal activity in an in vitro model of oral candidosis
PublicationOpportunistic oral infections caused by Candida albicans are frequent problems in immunocompromised patients. Management of such infections is limited due to the low number of antifungal drugs available, their relatively high toxicity and the emergence of antifungal resistance. Given these issues, our investigations have focused on novel derivatives of the antifungal antibiotic Nystatin A1, generated by modifications at the amino...
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A DFT computational study on the molecular mechanism of the nitro group migration in the product derived from 3-nitro-2-(trifluoromethyl)-2 H -chromene and 2-(1-phenylpropylidene)malononitrile
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Hypericum alpestre extract exhibits in vitro and in vivo anticancer properties by regulating the cellular antioxidant system and metabolic pathway of L‐arginine
PublicationConventional treatment methods are not effective enough to fight the rapid increase in cancer cases. The interest is increasing in the investigation of herbal sources for the development of new anticancer therapeutics. This study aims to investigate the antitumor capacity of Hypericum alpestre (H. alpestre) extract in vitro and in vivo, either alone or in combination with the inhibitors of the L‐arginine/polyamine/nitric oxide...
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Natural fish oil improves the differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes in vitro after interaction with the blood–brain barrier
PublicationThe blood–brain barrier (BBB) tightly controls the microenvironment of the central nervous system (CNS) to allow neurons to function properly. Additionally, emerging studies point to the beneficial effect of natural oils affecting a wide variety of physiological and pathological processes in the human body. In this study, using an in vitro model of the BBB, we tested the influence of natural fish oil mixture (FOM) vs. borage oil...
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Effect of oxidation and in vitro intestinal hydrolysis on phospholipid toxicity towards HT29 cell line serving as a model of human intestinal epithelium
PublicationOxidation of food-derived phospholipids (PLs) can influence nutrient digestion and induce oxidative stress in gastrointestinal epithelium. In this study, hen egg yolk PL fraction was used to evaluate the effect of lipoxygenase (LOX)-induced PL oxidation on the rate of PL hydrolysis catalyzed by pancreatic phospholipase A2 (PLA2) in the presence of bile salts (BSs). Then, PL/BS solutions containing native or oxidized PLs were used...
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In vitro affinity of Deinococcus radiodurans MutS towards mismatched DNA exceeds that of its orthologues from Escherichia coli and Thermus thermophilus
PublicationThe mismatch binding protein MutS is responsible for the recognition of mispaired and unpaired bases, which is the initial step in DNA repair. Among the MutS proteins most extensively studied in vitro are those derived from Thermus thermophilus, Thermus aquaticus and Escherichia coli. Here, we present the first report on the in vitro examination of DNA mismatch binding activity of MutS protein from Deinococcus radiodurans and confront...
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Is the presence of Central European strains of Raphidiopsis (Cylindrospermopsis) raciborskii a threat to a freshwater fish? An in vitro toxicological study in common carp cells
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Zinc Oxide Nanoparticles Impair the Integrity of Human Umbilical Vein Endothelial Cell Monolayer <I>In</I> <I>Vitro</I>
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Muscle Cell Morphogenesis, Structure, Development and Differentiation Processes Are Significantly Regulated during Human Ovarian Granulosa Cells In Vitro Cultivation
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Substrate profiling of Finegoldia magna SufA protease, inhibitor screening and application to prevent human fibrinogen degradation and bacteria growth in vitro
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Capping Agent-Dependent Toxicity and Antimicrobial Activity of Silver Nanoparticles: An In Vitro Study. Concerns about Potential Application in Dental Practice
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Sulfanyl porphyrazines: Molecular barrel-like self-assembly in crystals, optical properties and in vitro photodynamic activity towards cancer cells
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Microorganisms from starter and protective cultures - Occurrence of antibiotic resistance and conjugal transfer of tet genes in vitro and during food fermentation
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Design, synthesis and in vitro biological evaluation of a small cyclic peptide as inhibitor of vascular endothelial growth factor binding to neuropilin-1
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Antioxidant, anticancer and hepatoprotective activities of Cotoneaster horizontalis Decne extract as well as α-tocopherol and amygdalin production from in vitro culture
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Grazing bifurcation and chaotic oscillations of vibro-impact systems with one degree of freedom
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QSAR Analysis of Selected Antimicrobial Structures Belonging to Nitro-derivatives of Heterocyclic Compounds
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Jarosław Wawer dr hab. inż.
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A population of human brain cells expressing phenotypic markers of more than one lineage can be induced in vitro to differentiate into mesenchymal cells
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New Gene Markers of Angiogenesis and Blood Vessels Development in Porcine Ovarian Granulosa Cells during Short-Term Primary Culture In Vitro
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Epithelium morphogenesis and oviduct development are regulated by significant increase of expression of genes after long-term in vitro primary culture – a microarray assays
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‘Heart development and morphogenesis’ is a novel pathway for human ovarian granulosa cell differentiation during long‑term in�vitro cultivation‑a microarray approach
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Expression and cellular distribution of cyclin-dependent kinase 4 (Cdk4) and connexin 43 (Cx43) in porcine oocytes before and after in vitro maturation
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Differential expression of GDF9, TGFB1, TGFB2 and TGFB3 in porcine oocytes isolated from follicles of different size before and after culture in vitro
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Validated HPTLC method for determination of ledol and alloaromadendrene in the essential oil fractions of Rhododendron tomentosum plants and in vitro cultures and bioautography for their activity screening
PublicationRhododendron tomentosum (Ledum palustre) is a bog shrub used in traditional medicine for treatment of respiratory and rheumatic diseases. Due to the large variability of the chemical composition of its essential oil, depending on the habitat, the in vitro cultures were established as the alternative source of the volatile fraction. There is a need to monitor a quality of the field grown as well as in vitro plant material, especially...
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Characteristics of blond and red star ruby jaffa graefruits (citrus paradisi):results of the studies in vitro, in vivo and on patients suffering from atherosclerosis
PublicationCelem projektu, którego wyniki opisano w pracy byly badania dwóch odmian (jasna i czerwona) owoców grejfruta (in vitro i in vivo) stosowanych jako suplement diety pacjentów cierpiących na arterioskleroze. Stwierdzono, że zawartość związków bioaktywnych a w szczególności zwiazakow fenolowych i lepsze właściwosci przeciwutelniajace wykazuja owoce jasnej odmiany grajfruta. Stosowanie diety z dodatkiem owoców czerownego grejfruta w...
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Shape-Depended Biological Properties of Ag3PO4 Microparticles: Evaluation of Antimicrobial Properties and Cytotoxicity in In Vitro Model—Safety Assessment of Potential Clinical Usage
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Synthesis, physical–chemical properties and in vitro photodynamic activity against oral cancer cells of novel porphyrazines possessing fluoroalkylthio and dietherthio substituents
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Przemiany metaboliczne in vitro przeciwnowotworowej pochodnej imidazoakrydonu, C-1311, i jej wpływ na aktywność wybranych izoenzymów cytochromu P450
PublicationPrzedmiotem badań przedstawionych w niniejszej pracy jest aktywna przeciwnowotworowo pochodna należąca do grupy imidazoakrydonów, tj. C-1311, i jej analog 8-metoksylowy, związek C-1330, zsyntetyzowane w Katedrze Technologii Leków i Biochemii Politechniki Gdańskiej. Wstępne badania wykazały brak wyraźnej reaktywności C-1311 i C-1330 wobec wybranych rekombinantowych ludzkich izoenzymów cytochromu P450,...
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Which Suture to Choose in Hepato-Pancreatic-Biliary Surgery? Assessment of the Influence of Pancreatic Juice and Bile on the Resistance of Suturing Materials—In Vitro Research
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Vitamin and Carotenoid Intake and Outcomes of In Vitro Fertilization in Women Referring to an Italian Fertility Service: A Cross-Sectional Analysis of a Prospective Cohort Study
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Red Kale (Brassica oleracea L. ssp. acephala L. var. sabellica) Induces Apoptosis in Human Colorectal Cancer Cells In Vitro
PublicationThis article presents the results of studies investigating the effect of red kale (Brassica oleracea L. ssp. acephala L. var. sabellica) extract on cancer cells (HT-29). The cytotoxicity of the red kale extract was assessed using MTT and LDH assays, while qRT-PCR was employed to analyze the expression of genes associated with the p53 signaling pathway to elucidate the effect of the extract on cancer cells. Furthermore, HPLC-ESI-QTOF-MS/MS...
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Ocena współpracy pali Vibro z podłożem gruntowym na podstawie badań in-situ
PublicationW odniesieniu od obecnych trendów optymalnego projektowania obiektów inżynierskich, dąży się do określania rzeczywistych wartości współdziałania podłoża gruntowego i konstrukcji. Pale Vibro, należą do grupy pali przemieszczeniowych z poszerzoną podstawą, charakteryzują się bardzo dużą nośnością, szczególnie w gruntach niespoistych. Próbne obciążenia statyczne wskazują, że nośność pali Vibro jest znacznie większa niż zakładana...
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Prognozowanie pracy pali Vibro obciążonych osiowo z wykorzystaniem wyników sondowania CPT.
PublicationW odniesieniu do obecnych trendów optymalnego projektowania obiektów inżynierskich, dąży sie do określania rzeczywistych wartości współoddziaływania podłoża gruntowego i konstrukcji. Pale Vibro należą do grupy pali przemieszczeniowych z poszerzoną podstawą, charakteryzują się bardzo dużą nośnością, szczególnie w gruntach niespoistych. Próbne obciążenia statyczne (SPLT) wskazują, że nośność pali Vibro...
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METODYKA SZACOWANIA OSIADAŃ PALI VIBRO NA PODSTAWIE WYNIKÓW BADAŃ STATYCZNYCH CPT
PublicationPróbne obciążenia statyczne (SPLT) wskazują, że nośność pali Vibro jest znacznie większa niż zakładana na etapie projektu. Trudność, w wielu przypadkach, sprawia wyznaczenie nośności granicznej na podstawie próbnego obciążenia statycznego, ze względu na liniowy przebieg krzywej obciążenie - osiadanie. Praca zawiera analizę, której wynikiem jest metoda prognozowania krzywej obciążenie - osiadanie pali Vibro obciążanych osiowo,...
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Obliczanie nośności i osiadań pali Vibro na podstawie sondowania sondą statyczną.
PublicationOmówiono metodę bezpośredniego obliczania krzywej obciązenie - osiadanie na podstawie testu statycznego sondowania (CPT). W badaniach uwzględniono wyniki próbnych obciążeń oraz analizę statystyczną
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The bile salt content of human bile impacts on simulated intestinal proteolysis of β-lactoglobulin
PublicationThe gastrointestinal hydrolysis of food proteins has been portrayed in scientific literature to predominantly depend on the activity and specificity of proteolytic enzymes. Human bile has not been considered to facilitate proteolysis in the small intestine, but rather to assist in intestinal lipolysis. However, human bile can potentially influence proteins that are largely resistant to gastric digestion, and which are mainly hydrolysed...
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Searching for in vitro biomarkers of susceptibility to prostate and cervical cancers by analysis of chromosomal instability, γ-H2AX foci, polymorphisms in DNA repair genes and apoptosis
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