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Wyniki wyszukiwania dla: stimuli-sensitive copolymers
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Potential of Silver Nanoparticles in Overcoming the Intrinsic Resistance of Pseudomonas aeruginosa to Secondary Metabolites from Carnivorous Plants
PublikacjaCarnivorous plants are exemplary natural sources of secondary metabolites with biological activity. However, the therapeutic antimicrobial potential of these compounds is limited due to intrinsic resistance of selected bacterial pathogens, among which Pseudomonas aeruginosa represents an extreme example. The objective of the study was to overcome the intrinsic resistance of P. aeruginosa by combining silver nanoparticles (AgNPs)...
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Detection of the Plant Pathogen Pseudomonas Syringae pv. Lachrymans on Antibody-Modified Gold Electrodes by Electrochemical Impedance Spectroscopy
PublikacjaThe present work describes an impedimetric immunosensor for Pseudomonas syringae pv. lachrymans (Psl) detection. This pathogen infects many crop species causing considerable yield losses, thus fast and cheap detection method is in high demand. In the assay, the gold disc electrode was modified with 4-aminothiophenol (4-ATP), glutaraldehyde (GA), and anti-Psl antibodies, and free-sites were blocked with bovine serum albumin (BSA)....
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Light-improved glucose sensing on ordered Au-Ti heterostructure
PublikacjaNon-enzymatic electrochemical platforms sensitive towards glucose presence have attracted a worldwide attention during last decades. We report on influence of solar light onto response of gold-titanium heterostructures prepared via controllable approach. The material based on Au nanoparticles orderly distributed over the structured titanium foil was obtained by electrochemical anodization followed by chemical etching, magnetron...
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Enhanced Activity of P4503A4 and UGT1A10 Induced by Acridinone Derivatives C-1305 and C-1311 in MCF-7 and HCT116 Cancer Cells: Consequences for the Drugs’ Cytotoxicity, Metabolism and Cellular Response
PublikacjaActivity modulation of drug metabolism enzymes can change the biotransformation of chemotherapeutics and cellular responses induced by them. As a result, drug-drug interactions can be modified. Acridinone derivatives, represented here by C-1305 and C-1311, are potent anticancer drugs. Previous studies in non-cellular systems showed that they are mechanism-based inhibitors of cytochrome P4503A4 and undergo glucuronidation via UDP-glucuronosyltranspherase...
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Extremal thermal loading of a bifurcation pipe
PublikacjaThe subject of considerations is a spherical bifurcation pipe of a live steam made of steel P91, which is an element of a block of coal-fired power plant working with a 18K370 turbine. As experience shows, it is a very sensitive element of the boiler pipelines. An extreme work scenario for such a block has been adopted, in which the turbine is shutting down to a warm state three times in 24 hours. This is an action dictated by...
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Regulation of the First Committed Step in Lipopolysaccharide Biosynthesis Catalyzed by LpxC Requires the Essential Protein LapC (YejM) and HslVU Protease
PublikacjaWe previously showed that lipopolysaccharide (LPS) assembly requires the essential LapB protein to regulate FtsH-mediated proteolysis of LpxC protein that catalyzes the first committed step in the LPS synthesis. To further understand the essential function of LapB and its role in LpxC turnover, multicopy suppressors of ΔlapB revealed that overproduction of HslV protease subunit prevents its lethality by proteolytic degradation...
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Anticancer Imidazoacridinone C-1311 is Effective in Androgen-Dependent and Androgen-Independent Prostate Cancer Cells
PublikacjaAndrogen receptor (AR) plays a crutial role in prostate cancer (PCa) development and metastasis. Here, we reported potent anti-PCa activity of a small molecule imidazoacridinone C-1311. In AR-positive PCa cells, C-1311 was found to inhibit the transcriptional activity of AR uncovering a novel mechanism that may be relevant for its anticancer effect. Mechanistically, C-1311 decreased AR binding to prostate-specific antigen (PSA)...
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Relation of the polymorphism of cyp51A sequence and the susceptibility of Aspergillus fumigatus isolates to triazoles determined by commercial gradient test (Etest) and by reference methods
PublikacjaThe aim of this study was to evaluate the accuracy of commercial gradient test (Etest) in the detection of triazole resistant Aspergillus fumigatus isolates using reference microdilution methods and the analysis of sequences of the cyp 51A gene. The study was performed on twenty clinical isolates which were identified as Aspergillus fumigatus based on the DNA sequences of the ITS1-2 fragment of ribosomal DNA and the β-tubulin gene,...
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TaRh 2 B 2 and NbRh 2 B 2 : Superconductors with a chiral noncentrosymmetric crystal structure
PublikacjaIt is a fundamental truth in solid compounds that the physical properties follow the symmetry of the crystal structure. Nowhere is the effect of symmetry more pronounced than in the electronic and magnetic properties of materials—even the projection of the bulk crystal symmetry onto different crystal faces is known to have a substantial impact on the surface electronic states. The effect of bulk crystal symmetry on the properties...
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High Frequency Harmonics Emission in Smart Grids
PublikacjaPulse-width modulated rectifiers are nowadays commonly used for AC to DC power conversion. PWM rectification technology is very effective and allows for bidirectional power flow with the possibility of power factor improvement and low-order harmonic emission limitation. Unfortunately, employed PWM boost topology results with generation of input current harmonic distortions in a frequency range tightly correlated to modulation frequency....
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Determination of antifreeze substances in the airport runoff waters by solid-phase microextraction and gas chromatography–mass spectrometry method
PublikacjaA new method has been developed for the determination of antifreeze agents such as ethylene glycol (EG), propylene glycol (PG), and diethylene glycol (DEG) in the samples of airport runoff water. The method is based on headspace solid–phase microextraction (HS–SPME) of target analytes, which is coupled with gas chromatography–mass spectrometry (GC–MS). Until now, there was a lack of appropriate methodology for collecting reliable...
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Low Frequency Conducted Emissions of Grid Connected Static Converters
PublikacjaPulse-width modulated rectifiers are nowadays commonly used for AC to DC power conversion. PWM rectification technology is very effective and allows for bidirectional power flow with the possibility of power factor improvement and low-order harmonic emission limitation. Unfortunately, employed PWM boost topology results with generation of input current harmonic distortions in a frequency range tightly correlated to modulation frequency....
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Myrosinase activity in different plant samples; optimisation of measurement conditions for spectrophotometric and pH-stat methods
PublikacjaMyrosinase found in Brassicaceae plants, is the enzyme responsible for hydrolysis of glucosinolates. As a result a variety of biologically active metabolites are liberated, whose importance in crop protection and especially in cancer chemoprevention is rapidly gaining recognition. The growing practical application of glucosinolate degradation products requires that sensitive and reliable methods of myrosinase activity determination...
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Social Differentiation of the Perception and Human Tissues Donation for Research Purposes.
PublikacjaThe willingness to donate human biological material for research purposes is shaped by socio-cultural factors; however, there is a lack of studies analysing the social perception of different human tissues, which may affect such willingness. This study aimed to distinguish different sociocultural categories of human tissues and types of potential donors based on their willingness to donate material. Quantitative research was...
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Thermodynamics aspects of interactions between acridine derivatives and DNA
PublikacjaDNA is a molecular target for many anticancer and antiviral drugs. Therefore, a clear understanding of the interaction of small molecules with DNA is important in the rational design of ligands that can bind to DNA with high affinity and selectivity. There are several methods to investigate interactions between drug and DNA. Some of them measure changing into DNA structures, such as lengthening and untwisting of helix of DNA. Other...
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Broken rotor symptoms in the sensorless control of induction machine
PublikacjaThe purpose of this paper is to investigate the need for a universal method for sensorless controlled induction motor drive diagnosis. The increasing number of sensorless control systems in industrial applications require a universal method for the drive diagnosis, which provides reliable diagnostic reasoning independent of control system structure and state variables measurement or estimation method.Simulations and experimental...
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Occurrence of antimicrobial resistant bacteria in environment and the statistical analysis of this phenomenon
PublikacjaIntroduction: In this study the occurrence of antimicrobial resistance patterns among fecal indicators (Escherichia coli and Enterococcus spp.) was analyzed in water and wastewater samples. The trends in antimicrobial resistance were analyzed using basic statistical methods. Methods: Samples were obtained from two local watercourses (Oliwski Stream and Reda River) as well as from the wastewater treatment plant (WWTP) Gdansk - Wschod....
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Molecular Strategy for Survival at a Critical High Temperature in Eschierichia coli
PublikacjaThe molecular mechanism supporting survival at a critical high temperature (CHT) in Escherichia coli was investigated. Genome-wide screening with a single-gene knockout library provided a list of genes indispensable for growth at 47°C, called thermotolerant genes. Genes for which expression was affected by exposure to CHT were identified by DNA chip analysis. Unexpectedly, the former contents did not overlap with the latter except...
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Capillary Electrophoresis in determination of short - chain carboxylic acids in aqueous samples
PublikacjaAnalysis of pollution is extremely important especially in areas directly adjacent tothe residential district. Studies show that short- chain carboxylic acids are a group ofcompounds widespread around sewage treatment plants, landfills, livestock farms. The mostcommon techniques for determination of short - chain carboxylic acids are chromatographictechniques, especially gas chromatography, whose main limitation is that only volatilemonocarboxylic...
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MnWO4/reduced graphene oxide-based electrochemical sensing platform for simultaneous detection of catechol and resorcinol
Publikacjan this study, a novel electrochemical sensor for accurate and sensitive catechol determination was demonstrated employing a screen-printed graphite electrode (SPGE) modified with MnWO4/reduced graphene oxide (MnWO4/rGO) nanocomposite. The MnWO4/rGO nanocomposite has been successfully prepared by using hydrothermal technique, and it was then characterized using several microscopic and spectroscopic methods (XRD, FE-SEM, and EDS)....
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STUDY ON THE RELATIONSHIP BETWEEN VEHICLE MAINTENANCE AND FUEL CONSUMPTION
PublikacjaA contemporary road vehicle (RV) is a rather complex system, consisting of a large number of subsystems, assemblies, units, and elements (parts). While operating, an RV interacts with the environment, and its elements interact with each other. Consequently, the properties (parameters) of these elements change in the process - hardness, roughness, size, relative position, gapping, etc. A partial solution to the presented problems...
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Potentiometric sensor containing set of ion selective electrodes with lipid modified membranes for quality assessment of tested non-alcoholic beverages
PublikacjaPotentiometric sensors may be a good tool for fast quality control of commercial drinks. In this work, such a sensor, containing a set of ion-selective electrodes with lipid-modified membranes (benzylhexadecyldimethylammonium chloride monohydrate, hexadecylamine, 1-dodecanol, elaidic acid, cholesterol) was used for discrimination and quality control of non-alcoholic beverages, mainly composed of sugar and citric acid. It was found...
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Deterministic versus stochastic modelling of unsaturated flow in a sandy field soil based on dual tracer breakthrough data
PublikacjaThe 216 km2 Neuenhagen Millcreeck catchment can be characterized as a drought sensitive landscape in NE Germany. It is therefore a fundamental human interest to understand how water that fell as precipitation moves through the unsaturated soils and recharges groundwater. Additionally, a better knowledge of nutrient transport from soil to groundwater is important also, especially in landscapes with light sandy soils. For a better...
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The effects of gas exposure on the graphene/AlGaN/GaN heterostructure under UV irradiation
PublikacjaThis work demonstrates a graphene/AlGaN/GaN sensing device with two-dimensional electron gas (2DEG) toward nitrogen dioxide (NO2), tetrahydrofuran, and acetone detection under UV light irradiation. We propose combining measurements of the DC characteristics with a fluctuation-enhanced sensing method to provide insight into the gas detection mechanisms in the synergistic structure of highly stable GaN and gas-sensitive graphene....
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Homogeneous nucleation rate of methane hydrate formation under experimental conditions from seeding simulations
PublikacjaIn this work, we shall estimate via computer simulations the homogeneous nucleation rate for the methane hydrate at 400 bars for a supercooling of about 35 K. The TIP4P/ICE model and a Lennard-Jones center were used for water and methane, respectively. To estimate the nucleation rate, the seeding technique was employed. Clusters of the methane hydrate of different sizes were inserted into the aqueous phase of a two-phase gas–liquid...
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Unveiling the Pool of Metallophores in Native Environments and Correlation with Their Potential Producers
PublikacjaFor many organisms, metallophores are essential biogenic ligands that ensure metal scavenging and acquisition from their environment. Their identification is challenging in highly organic matter rich environments like peatlands due to low solubilization and metal scarcity and high matrix complexity. In contrast to common approaches based on sample modification by spiking of metal isotope tags, we have developed a two-dimensional...
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Electro-optical transducer based on indium-tin-oxide-coated optical fiber for analysis of ionized media
PublikacjaThe paper introduces a concept of an optical fiber based electro-optical transducer for monitoring of ionized media, such as low-temperature plasma. It utilizes optical fiber with a section of a core coated with tailored indium tin oxide (ITO) thin film and thus combines the optical phenomena of lossy-mode resonance (LMR) with the electrostatic probe. ITO is an optically transparent and electrically conductive material and if its...
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Synthesis of 3-(2-Alkylthio-4-chloro-5-methylbenzenesulfonyl)-2-(1-phenyl-3-arylprop-2-enylideneamino)guanidine Derivatives with Pro-Apoptotic Activity against Cancer Cells
PublikacjaThe untypical course of reaction between chalcones and benzenesulfonylaminoguanidines led to the new 3-(2-alkylthio-4-chloro-5-methylbenzenesulfonyl)-2-(1-phenyl-3-arylprop-2- enylideneamino)guanidine derivatives 8–33. The new compounds were tested in vitro for their impact on the growth of breast cancer cells MCF-7, cervical cancer cells HeLa and colon cancer cells HCT-116 by MTT assay. The results revealed that the activity of...
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N-doped carbon nanospheres as selective fluorescent probes for mercury detection in contaminated aqueous media: chemistry, fluorescence probing, cell line patterning, and liver tissue interaction
PublikacjaA precise nano-scale biosensor was developed here to detect Hg2+ in aqueous media. Nitrogen-doped carbon nanospheres (NCS) created from the pyrolysis of melamine–formaldehyde resin were characterized by FESEM, XRD, Raman spectra, EDS, PL, UV–vis spectra, and N2 adsorption–desorption, and were used as a highly selective and sensitive probe for detecting Hg2+ in aqueous media. The sensitivity of NCS to Hg2+ was evaluated by photoluminescence...
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Material Design and Optimisation of Electrochemical Li-Ion Storage Properties of Ternary Silicon Oxycarbide/Graphite/Tin Nanocomposites
PublikacjaIn this work, we present the characterization and electrochemical performance of various ternary silicon oxycarbide/graphite/tin (SiOC/C/Sn) nanocomposites as anodes for lithium-ion batteries. In binary SiOC/Sn composites, tin nanoparticles may be produced in situ via carbothermal reduction of SnO2 to metallic Sn, which consumes free carbon from the SiOC ceramic phase, thereby limiting the carbon content in the final ceramic nanocomposite....
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Fast Real-Time RDFT- and GDFT-Based Direct Fault Diagnosis of Induction Motor Drive
PublikacjaThis paper presents the theoretical analysis and experimental verification of a direct fault harmonic identification approach in a converter-fed electric drive for automated diagnosis purposes. On the basis of the analytical model of the proposed real-time direct fault diagnosis, the fault-related harmonic component is calculated using recursive DFT (RDFT) and Goertzel DFT (GDFT), applied instead of the full spectrum calculations...
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An Impact Analysis of Artificial Light at Night (ALAN) on Bats. A Case Study of the Historic Monument and Natura 2000 Wisłoujście Fortress in Gdansk, Poland
PublikacjaThe artificial light at night (ALAN) present in many cities and towns has a negative impact on numerous organisms that live alongside humans, including bats. Therefore, we investigated if the artificial illumination of the historic Wisłoujście Fortress in Gdańsk, Poland (part of the Natura 2000 network), during nighttime events, which included an outdoor electronic dance music (EDM) festival, might be responsible for increased...
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Spatial Differences in the Chemical Composition of Surface Water in the Hornsund Fjord Area: A Statistical Analysis with A Focus on Local Pollution Sources
PublikacjaSurface catchments in Svalbard are sensitive to external pollution, and yet what is frequently considered external contamination may originate from local sources and natural processes. In this work, we analyze the chemical composition of surface waters in the catchments surrounding the Polish Polar Station in Svalbard, Hornsund fjord area. We have pooled unpublished and already published data describing surface water composition...
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Public art in the era of urban transformations. A case study of Eduardo Paolozzi’s artistic output in Edinburgh and in London
PublikacjaUrban space dedicated to cultural life, entertainment or business is currently exposed to dynamic urbanistic transformations. Monumental works of art within the boundaries of cities, for years embedded in the urban landscape and awareness of viewers, are moved to other places or even destroyed. Decisions about a change of their location and context, which are fundamental aspects for the impression made by a given work of art, cause...
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Real-time isothermal DNA amplification monitoring in picoliter volumes using an optical fiber sensor
PublikacjaRolling circle amplification (RCA) of DNA can be considered as a great alternative to the gold standard polymerase chain reaction (PCR), especially during this pandemic period, where rapid, sensitive, and reliable test results for hundreds of thousands of samples are required daily. This work presents the first research to date on direct, real-time and label-free isothermal DNA amplification monitoring using a microcavity in-line...
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Functional safety and cyber security analysis for life cycle management of industrial control systems in hazardous plants and oil port critical infrastructure including insurance
PublikacjaThis report addresses selected methodological aspects of proactive reliability, functional safety and cyber security management in life cycle of industrial automation and control systems (IACS) in hazardous plants and oil port critical installations based on the analysis of relevant hazards / threats and evaluation of related risks. In addition the insurance company point of view has been also considered, because nowadays the insurer,...
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Metoda i algorytmy sterowania procesami miksowania dźwięku za pomocą gestów w oparciu o analizę obrazu wizyjnego
PublikacjaGłównym celem rozprawy było opracowanie systemu miksowania dźwięku za pomocą gestów rąk wykonywanych w powietrzu oraz zbadanie możliwości oferowanych przez takie rozwiązanie w porównaniu ze współczesną metodą miksowania sygnałów fonicznych, wykorzystującą środowisko komputera. Opracowany system rozpoznaje zarówno dynamiczne jak i statyczne gesty rąk. Rozpoznawanie gestów dynamicznych zrealizowano w oparciu o metody logiki rozmytej...
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Mechanics of Micro- and Nano-Size Materials and Structures
PublikacjaNanotechnology knowledge is always looking to expand its boundaries to achieve the mostsignificant benefit to human life and meet the growing needs of today. In this case, we can refer tomicro- and nanosensors in micro/nano-electromechanical systems (MEMS/NEMS). These electricaldevices can detect minimal physical stimuli up to one nanometer in size. Today, micro/nano-sensordevices are widely used in the...
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Mechanisms of Damage to DNA Labeled with Electrophilic Nucleobases Induced by Ionizing or UV Radiation
PublikacjaHypoxia—a hallmark of solid tumors—makes hypoxic cells radioresistant. On the other hand, DNA, the main target of anticancer therapy, is not sensitive to the near UV photons and hydrated electrons, one of the major products of water radiolysis under hypoxic conditions. A possible way to overcome these obstacles to the efficient radio- and photodynamic therapy of cancer is to sensitize the cellular DNA to electrons and/or ultraviolet...
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Simulations of freshwater lens recharge and salt/freshwater interfaces using the HYDRUS and SWI2 packages for MODFLOW
PublikacjaThe paper presents an evaluation of the combined use of the HYDRUS and SWI2 packages for MODFLOW as a potential tool for modeling recharge in coastal aquife rs subject to saltwater intrusion. The HYDRUS package for MODFLOW solves numerically the one-dimensional form of the Richards equation describing water flow in variably- saturated media. The code computes groundwater recharge to or...
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Time-frequency analysis in optical coherence tomography for technical objects examination
PublikacjaOptical coherence tomography (OCT) is one of the most advanced optical measurement techniques for complex structure visualization. The advantages of OCT have been used for surface and subsurface defect detection in composite materials, polymers, ceramics, non-metallic protective coatings, and many more. Our research activity has been focused on timefrequency spectroscopic analysis in OCT. It is based on time resolved spectral analysis...
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Diagnostic PCR assay for Microsporum and Trichophyton infections
PublikacjaWe have previously described a highly sensitive 5-hour PCR test for the rapid diagnosis of onychomycosis which detects any dermatophyte and species-identify T. rubrum from patient specimens. We have subsequently developed and evaluated new PCR tests for detection of Trichophyton and Microsporum canis/audouinii infections.58 dermatophyte isolates (21) Microsporum spp. (4 different species), 35 Trichophyton spp (10 different species)...
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Phospholipid-functionalized gold electrode for cellular membrane interface studies - interactions between DMPC bilayer and human cystatin C
PublikacjaThis work describes the electrochemical studies on the interactions between V57G mutant of human cystatin C (hCC V57G) and membrane bilayer immobilized on the surface of a gold electrode. The electrode was modified with 6-mercaptohexan-1-ol (MCH) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). DMPC was used as a membrane mimetic for monitoring electrochemical changes resulting from the interactions between the functionalized...
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Magnetic field mapping along a NV-rich nanodiamond-doped fiber
PublikacjaIntegration of NV−-rich diamond with optical fibers enables guiding quantum information on the spin state of the NV− color center. Diamond-functionalized optical fiber sensors have been demonstrated with impressive sub-nanotesla magnetic field sensitivities over localized magnetic field sources, but their potential for distributed sensing remains unexplored. The volumetric incorporation of diamonds into the optical fiber core allows...
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Brain circuit imprints of developmental 17α-Ethinylestradiol exposure in guppies (Poecilia reticulata): Persistent effects on anxiety but not on reproductive behaviour
PublikacjaThe effects of endocrine disruptors may vary with the timing of exposure. The physiological implications of adult exposure are present during and shortly after exposure while embryonic exposure can imprint changes manifested in adulthood. In this study, guppy (Poecilia reticulata) embryos were exposed to 2 and 20 ng/L of 17α-ethinylestradiol during development via the mother and reared in clean water from gestation until 6 months...
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Impact of Climate Change on Water Sources and River‐Floodplain Mixing in the Natural Wetland Floodplain of Biebrza River
PublikacjaThe origins of river and floodplain waters (groundwater, rainfall, and snowmelt) and their extent during overbank flow events strongly impact ecological processes such as denitrification and vegetation development. However, the long-term sensitivity of floodplain water signatures to climate change remains elusive. We examined how the integrated hydrological model HydroGeoSphere and the Hydraulic Mixing-Cell method could help us...
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Spatial variability of the hydrochemistry of shallow groundwaters and surface waters of the Rensdyrbekken: A case study of a permafrost catchment in Bellsund (SW Spitsbergen, Svalbard)
PublikacjaProgressive climate change may have unpredictable consequences for the Arctic environment. Permafrost catchments off the west coast of Svalbard, described as “thin” and “warm,” are particularly sensitive to climate change. The interdisciplinary research on the hydrochemical response of surface and underground water functioning within a small permafrost catchment area focused on the determination of the impact of meteorological...
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Anisotropy and compaction gradient assessment on rammed earth specimens through sonic tomography approach
PublikacjaRammed earth is a traditional construction technique that has recently gained attention because of its benefits from an ecological perspective. The conservation of the existing valuable cultural heritage sites and the quality control of new constructions built with this material require the development and application of practical inspection techniques. This paper explores the application of sonic tests and sonic tomography as...
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Electrochemistry/mass spectrometry (EC/MS) for fast generation and identification of novel reactive metabolites of two unsymmetrical bisacridines with anticancer activity.
PublikacjaThe development of a new drug requires knowledge about its metabolic fate in a living organism, regarding the comprehensive assessment of both drug therapeutic activity and toxicity profiles. Electrochemistry (EC) coupled with mass spectrometry (MS) is an efficient tool for predicting the phase I metabolism of redox-sensitive drugs. In particular, EC/MS represents a clear advantage for the generation of reactive drug transformation...
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Temperature influences on shear stability of a nanosize plate with piezoelectricity effect
PublikacjaPurpose The purpose of this paper is to predict the mechanical behavior of a piezoelectric nanoplate under shear stability by taking electric voltage into account in thermal environment. Design/methodology/approach Simplified first-order shear deformation theory has been used as a displacement field. Modified couple stress theory has been applied for considering small-size effects. An analytical solution has been taken into account...