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Mapping the Structural and Dynamic Determinants of pH-Sensitive Heparin Binding to Granulocyte Macrophage Colony Stimulating Factor
PublikacjaGranulocyte macrophage colony stimulating factor (GMCSF) is an immunomodulatory cytokine that is harnessed as a therapeutic. GMCSF is known to interact with other clinically important molecules, such as heparin, suggesting that endogenous and administered GMCSF has the potential to modulate orthogonal treatment outcomes. Thus, molecular level characterization of GMCSF and its interactions with biologically active compounds is critical...
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Verification of Satellite Railway Track Position Measurements Making Use of Standard Co-Ordinate Determination Techniques
PublikacjaThe article presents the results of satellite railway track position measurements performed by a multidisciplinary research team, the members of which represented Gdansk University of Technology and Gdynia Maritime University. Measuring methods are described which were used for reconstructing the railway track axis position and diagnosing railway track geometry deformations. As well as that, the description of the novel method...
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Reversible Data Hiding in Encrypted DICOM Images Using Cyclic Binary Golay (23, 12) Code
PublikacjaIn this paper, a novel reversible data hiding method for encrypted images (RDHEI) is proposed. An efficient coding scheme based on cyclic binary Golay (23, 12) code is designed to embed additional data into the least significant bits (LSBs) of the encrypted image. The most significant bits (MSBs) are used to ensure the reversibility of the embedding process. The proposed scheme is lossless, and based on the receiver’s privileges,...
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Linking optical and electronic properties to photoresponse of heterojunctions based on titania nanotubes and chromium, molybdenum, and tungsten oxides
PublikacjaThe development of photosensitization strategies for titanium dioxide is necessary for the enhancement of its optical and electronic properties towards its application potential in solar photoelectrochemistry. In this work, significant differences in the photosensitizing capability of the 6th group transition metal oxides applied on the surface of titania nanotubes are reported. For the first time, correlations between the experimentally...
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Highly Conserved Homotrimer Cavity Formed by the SARS-CoV-2 Spike Glycoprotein: A Novel Binding Site
PublikacjaAn important stage in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) life cycle is the binding of the spike (S) protein to the angiotensin converting enzyme-2 (ACE2) host cell receptor. Therefore, to explore conserved features in spike protein dynamics and to identify potentially novel regions for drugging, we measured spike protein variability derived from 791 viral genomes and studied its properties by molecular...
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A method of predicting the best conditions for large-size workpiece clamping to reduce vibration in the face milling process
PublikacjaThe paper presents an innovative method of solving the problem of vibration suppression during milling of large-size details. It consists in searching for the best conditions for clamping the workpiece based on a rapid modal identification of the dominant natural frequencies only and requires repetitive changes in the tightening torque of the clamping screws. Then, by estimating the minimum work of the cutting forces acting in...
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DESIGN OF THE DUAL CONSTELLATION GPS/GALILEO MOBILE DEVICE FOR IMPROVING NAVIGATION OF THE VISUALLY IMPAIRED IN AN URBAN AREA, POLISH MARITIME RESEARCH
PublikacjaThe article discusses damages of essential tribological associations in crankshaft and piston systems of large power two-stroke engines used as main engines, which take place during transport tasks performed by those ships. Difficulties are named which make preventing those damages impossible, despite the fact that the technical state of engines of this type is identified with the aid of complex diagnostic systems making use of...
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Why do G-quadruplexes dimerize through the 5’-ends? Driving forces for G4 DNA dimerization examined in atomic detail
PublikacjaG-quadruplexes (G4) are secondary structures formed by guanine-rich nucleic acid sequences and shown to exist in living cells where they participate in regulation of gene expression and chromosome maintenance. G-quadruplexes with solvent-exposed guanine tetrads show the tendency to associate together through cofacial stacking, which may be important for packaging of G4-forming sequences and allows for the design of higher-order...
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Novel DNA-binding protein from Nanoarchaeum equitans Kin4-M binds all kinds of nucleic acids
PublikacjaNanoarchaeum equitans is the only known representative of Archaea phylum Nanoarchaeota and stands out as one of the tiniest known living organism. What is more it has smallest genome, which is only 490.885 base pairs long. It is also one of the most compact genomes. According to predictions about 95% of the DNA encodes proteins or stable RNA. Nanoarchaeum equitans lacks genes for most vital metabolic pathways including lipid, cofactor,...
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Influence of temperature and interactions with ligands on dissociation of dsDNA and ligand-dsDNA complexes of various types of binding : an electrochemical study.
PublikacjaSeveral medicinally important compounds that bind to dsDNA strands via intercalation (C-1311, C-1305, EtBr), major groove binding (Hoechst 33258) and covalent binding (cis-Pt) were examined. The obtained results suggest that both the transfer of conformation B to C and the denaturation process, for the ligand-dsDNA complexes, except for covalently bound cis-Pt, took place at higher temperatures compared to the unbound helix. Furthermore,...
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Application of reversed-phase thin layer chromatography and QSRR modelling for prediction of protein binding of selected β-blockers
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Praca z drewnem - Przestrzeń eksperymentu w tworzeniu architektury; Crafting Wood - Space of experiment in making architecture
PublikacjaW artykule przedstawiono eksperymenty z materiałem w tworzeniu architektury prowadzące do praktycznych rozwiązań w konstrukcji budynku. Takie podejście do tematyki zastosowania materiałów w architekturze nakreśla praktyczne aspekty tworzenia przestrzeni podczas pracy np. z drewnem. Podejście przedstawiono w kontekście dziedzictwa kulturowego podczas intensywnych warsztatów dotyczących tradycji i kultury budownictwa wybranych regionów...
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Study on metrological relations between instant tool displacements and surface roughness during precise ball end milling
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Influence of redundancy and ship machinery crew manning on reliability of lubricating oil system for the MC-Type diesel engine
PublikacjaW referacie przedstawiono wyniki rozważań na temat wpływu zastosowania redundancji i prewencyjnych działań załogi na niezawodność instalacji oleju smarowego dla silników spalinowych, wysokoprężnych typu MC. Uwzględniono przy tym trzy poziomy wyszkolenia załogi: wysoki, przeciętny oraz niski.
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Reducing the Uncertainty of the Moving Object Location Measurement with the Method of Quasi-Multiple Measurement in GNSS Technology in Symmetrical Arrangement
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Modelling assisted phytoremediation of soils contaminated with heavy metals – Main opportunities, limitations, decision making and future prospects
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Cost of Equity of Coal-Fired Power Generation Projects in Poland: Its Importance for the Management of Decision-Making Process
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918 The role of patients and doctors in making decisions about the choice of the kind of adjuvant treatment in early breast cancer
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260. The role of patients and doctors in making decisions about the choice of the kind of adjuvant treatment in early brest cancer
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Insight into the microbial and genetic response of anammox biomass to broad range concentrations of different antibiotics: Linking performance and mechanism
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Making European Muslims: Religious Socialization among Young Muslims in Scandinavia and Western Europe, edited by Mark Sedgwick
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Predicting Value of Binding Constants of Organic Ligands to Beta-Cyclodextrin: Application of MARSplines and Descriptors Encoded in SMILES String
PublikacjaThe quantitative structure–activity relationship (QSPR) model was formulated to quantify values of the binding constant (lnK) of a series of ligands to beta–cyclodextrin (β-CD). For this purpose, the multivariate adaptive regression splines (MARSplines) methodology was adopted with molecular descriptors derived from the simplified molecular input line entry specification (SMILES) strings. This approach allows discovery of regression...
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Do Involvement in Alcoholics Anonymous and Religiousness Both Directly and Indirectly through Meaning in Life Lead to Spiritual Experiences?
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Twenty-four hour and early morning blood pressure control of olmesartan vs. ramipril in elderly hypertensive patients
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Optimization of substrate-lens-coupled CMOS field-effect transistor detectors for 250 GHz by pixel binning technique
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Characterization of bacterial structures in two-stage moving-bed biofilm reactor (MBBR) during nitrification of the landfill leachate
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Study of the influence of high-energy milling time on the Cu–13Al–4Ni alloy manufactured by powder metallurgy process
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Analysis of Procollagen C-Proteinase Enhancer-1/Glycosaminoglycan Binding Sites and of the Potential Role of Calcium Ions in the Interaction
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The optimal control at energy performance index as a rule for vibration surveillance in the hybrid system by on example of milling flexible details
PublikacjaPraca dotyczy nadzorowania drgań w niestacjonarnych układach mechanicznych za pomocą sterowania optymalnego pry energetycznym wskaźniku jakości. W układzie drgającym wyodrębniono podukłady: modalny, strukturalny oraz łączący. Dynamikę układu sterowanego opisano we współrzędnych hybrydowych. Przedstawiono przykład nadzorowania drgań podczas obróbki przedmiotów podatnych smukłym frezem kulistym. Paramatry obliczeniowego modelu modalnego...
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Optimization of clamping stiffness during milling of high-dimensional structures with the use of techniques of experiment – aided virtual prototyping
PublikacjaThe subject of this paper is a method of searching for conditions of minimizing the vibration level of a tool-high dimensional flexible workpiece, at unchangeable technological parameters of the machining process. It depends on repeatable change of the values of the stiffness coefficients as soon as an optimal vibration state of the workpiece approaches. There are assessed the values of dominant ”peaks” in the frequency spectra...
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Move into the open space – what can we expect from moving artistic interventions to open urban spaces?
PublikacjaThe paper discuses the role of incorporating creative engagement into the processes of urban and social development using cultural means of action and expression and the potential of artistic interventions in public space. The block of initiatives within the People’s Smart Sculpture EU project, realized by Gdańsk City Gallery, has been named “Move Into The Open Space”. This paper will provide...
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Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization
PublikacjaDNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules. They are used in various fields of science, predominantly as essential components for in vitro DNA syntheses, known as PCR. Modern diagnostics, molecular biology and genetic engineering need DNA polymerases which demonstrate improved performance. This study was aimed at obtaining a new NeqSSB-TaqS fusion DNA polymerase from the...
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NUMERICAL SIMULATIONS OF HONING PROCESS OF THIN WALL CYLINDER LINERS, WITH CONSTANT AND WITH VARIABLE THICKNESS OF WALL OF HONED PARTS
PublikacjaNumerical simulations of honing process of thin-wall cylinder liners, with constant and with variable thickness of the wall of honed workpieces can improve and can help to conduct the experimental research of honing process and can improve the honing process. A very valuable research assumption, before performing the numerical analysis of computer simulation of honing process, is the measurement of the real geometry of the honing...
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Molecular mechanism and energetics of coupling between substrate binding and product release in the F 1 -ATPase catalytic cycle
PublikacjaF1-ATPase is a motor protein that couples the rotation of its rotary γ subunit with ATP synthesis or hydrolysis. Single-molecule experiments indicate that nucleotide binding and release events occur almost simultaneously during the synthesis cycle, allowing the energy gain due to spontaneous binding of ADP to one catalytic β subunit to be directly harnessed for driving the release of ATP from another rather than being dissipated...
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The Application of a Multi-Criteria Decision-Making for Indication of Directions of the Development of Renewable Energy Sources in the Context of Energy Policy
PublikacjaThis paper presents the application of multi-criteria decision-making (MCDM) for evaluating what technologies using renewable energy sources (RES) for electricity production have the chance to develop in Poland under the current socio-economic conditions. First, the Analytical Hierarchy Process (AHP) method was used to determine the weights of the optimization criteria. Five main criteria and 30 sub-criteria were identified. Next,...
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Optimization of the spindle speed during milling of large-sized structures with the use of technique of Experiment-Aided Virtual Prototyping
PublikacjaIn the paper are presented considerations concerning vibration suppression problems during milling of large-sized workpieces with the use of innovative method of matching the spindle speed of cutting tool. It depends on repeatable change of the spindle speed value as soon as the optimal vibration state of the workpiece approaches. The values of dominant “peaks” in the frequency spectra and the Root Mean Square (RMS) values of time...
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ALTERNATIVE, DIRECT SYNTHESIS METHOD OF THE CERAMIC SOLID SOLUTIONS BASED ON BaTiO3 THROUGH A HIGH ENERGY BALL MILLING
PublikacjaPolycrystalline samples BaTiO3 and the solid solutions Ba0.9Sr0.1TiO3, Ba0.9Sr0.1Ti0.9Sn0.1O3, Ba0.9Sr0.1Ti0.8Sn0.2O3 were obtained by means of a mechanochemical treatment based on the high-energy ball milling technique and next a high temperature solid state reaction method. The influence of synthesis condition on microstructural, dielectric and ferroelectric properties of obtained solid solutions were investigated. The structure...
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Study of a Multicriterion Decision-Making Approach to the MQL Turning of AISI 304 Steel Using Hybrid Nanocutting Fluid
PublikacjaThe enormous use of cutting fluid in machining leads to an increase in machining costs, along with different health hazards. Cutting fluid can be used efficiently using the MQL (minimum quantity lubrication) method, which aids in improving the machining performance. This paper contains multiple responses, namely, force, surface roughness, and temperature, so there arises a need for a multicriteria optimization technique. Therefore,...
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Experimentally Aided Operational Virtual Prototyping to Predict Best Clamping Conditions for Face Milling of Large-Size Structures
PublikacjaVibrations occurring during milling operations are one of the main issues disturbing the pursuit of better efficiency of milling operations and product quality. Even in the case of a stable cutting process, vibration reduction is still an important goal. One of the possible solutions to obtain it is selection of the favorable conditions for clamping the workpiece to the machine table. In this paper, a method for predicting and...
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Reducing the Uncertainty of the Moving Object Location Measurement with the Method of Quasi-Multiple Measurement in GNSS Technology in Symmetrical Arrangement
PublikacjaThe article presents a solution to the problem of limited accuracy of dynamic measurements performed with GNSS receivers. The proposed measurement method is a response to the needs related to the assessment of the measurement uncertainty of the position of the track axis of the rail transport line. However, the problem of reducing the measurement uncertainty is universal for many different situations where high accuracy of positioning...
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Light pollution from illuminated bridges as a potential barrier for migrating fish–Linking measurements with a proposal for a conceptual model
PublikacjaIlluminated bridges have become important assets to navigable aquatic systems. However, if artificial light at night (ALAN) from illuminated bridges reaches aquatic habitats, such as rivers, it can threaten the river's natural heterogeneity and alter the behavioural responses of migratory fish. Here, via a pilot study, we quantified levels of ALAN at illuminated bridges that cross a river and, propose a conceptual model to estimate...
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Cross-Linking, Morphology, and Physico-Mechanical Properties of GTR/SBS Blends: Dicumyl Peroxide vs. Sulfur System
PublikacjaIn this work, ground tire rubber and styrene–butadiene block copolymer (GTR/SBS) blends at the ratio of 50/50 wt%, with the application of four different SBS copolymer grades (linear and radial) and two types of cross-linking agent (a sulfur-based system and dicumyl peroxide), were prepared by melt compounding. The rheological and cross-linking behavior, physico-mechanical parameters (i.e., tensile properties, abrasion resistance,...
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Identification, cloning, expression, and characterizationof a highly thermostable single-stranded-DNA-binding protein (SSB) from Deinococcus murrayi
PublikacjaZidentyfikowano i scharakteryzowano białko SSB-podobne, pochodzące z Deinococcus murrayi (DmuSSB). Otrzymany w reakcji PCR fragment zawierający kompletny gen ssb sklonowano w systemie ekspresyjnym Escherichia coli. Gen składa się z 826 nt, kodujących 276 reszt aminokwasowych z wyliczoną teoretycznie masą cząsteczkową monomeru równą 30,14 kDa. DmuSSB zawiera dwie domeny wiążące jednoniciowe DNA OBna monomer I funkcjonuje jako homodimer....
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T cell Activation Is Driven by an ADP-Dependent Glukosinase Linking Enhanced Glycolysis with Mitochondrial Reactive Oxygen Species Generation
PublikacjaMitochondria-originating reactive oxygen species control T cell receptor (TCR)-induced gene expression. Here, we show that TCR-triggered activation of ADP-dependent glucokinase (ADPGK), an alternative, glycolytic enzyme typical for Archaea, mediates generation of the oxidative signal. We also show that ADPGK is localized in the endoplasmic reticulum and suggest that its active site protrudes toward the cytosol. The ADPGK-driven...
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Vibration surveillance during ball end milling of curved flexible details on a basis of assessment of assurance of the model of real object
PublikacjaW pracy przedstawiono nową metodę nadzorowania drgań podczas frezowania zakrzywionych przedmiotów podatnych. Wykorzystano model modalny przedmiotu obrabianego, którego parametry zidentyfikowano na bazie badań doświadczalnych oraz analizy komputerowej. Zastosowanie modelu metody elementów skończonych, którego zachowanie jest zgodne z przedmiotem uformowanym w postaci cienkościennej powierzchni półwalcowej, a następnie - utworzenie...
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Physicochemical profile of Os (III) complexes with pyrazine derivatives: From solution behavior to DNA binding studies and biological assay
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Cross-linking of sodium caseinate-structured emulsion with transglutaminase alters postprandial metabolic and appetite responses in healthy young individuals
PublikacjaThe physico-chemical and interfacial properties of fat emulsions influence lipid digestion and may affect postprandial responses. The aim of the present study was to determine the effects of the modification of the interfacial layer of a fat emulsion by cross-linking on postprandial metabolic and appetite responses. A total of fifteen healthy individuals (26·5 (sem 6·9) years and BMI 21·9 (sem 2·0) kg/m2) participated in a cross-over...
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Modeling flatness deviation in face milling considering angular movement of the machine tool system components and tool flank wear
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Application of signal to noise ratio and grey relational analysis to minimize forces and vibrations during precise ball end milling
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Effect of ball-milling process parameters on mechanical properties of Al/Al2O3/collagen powder composite using statistical approach
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