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Search results for: ANTIOXIDANT ENZYMES
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Determination of Antioxidant Activity of Vitamin C by Voltammetric Methods
PublicationVoltammetric methods—cyclic (CV) and differential pulse voltammetry (DPV) are considered the most appropriate way to evaluate antioxidant activity of redox active compounds. They provide information about both mechanism and kinetics of electrochemical oxidation of antioxidants as well as their physical and chemical properties such as the redox potential or the number of electrons transferred. These methods are helpful for understanding...
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Endogenous oxidative DNA base modifications analysed with repair enzymes and GC/MS technique
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KU812 basophils express urocortin, CRH-R, MC1R and steroidogenic enzymes and secrete progesterone
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Cuticle hydrolysis in four medically important fly species by enzymes of the entomopathogenic fungusConidiobolus coronatus
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Simultaneous siRNA-mediated silencing of pairs of genes coding for enzymes involved in glycosaminoglycan synthesis.
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The influence of fresh cabbage and sauerkraut juices on the activity of protective enzymes in human cell lines
PublicationMechanizmami przeciwrakotwórczego działania związków zawartych w kapuście jest m.in. metaboliczna aktywacja enzymów II fazy detoksykacji, indukcja enzymów naprawczych DNA oraz aktywnośc przeciwutleniająca. Do enzymów II fazy odtruwania należą m.in. S-transferazy glutationowe (GST) oraz oksydoreduktaza chinonowa NQO1 zwana też DT-diaforazą. GST katalizują reakcję sprzęgania glutationu z wieloma różnymi typami ksenobiotyków, ułatwiając...
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Structural insights, biocatalytic characteristics, and application prospects of lignin-modifying enzymes for sustainable biotechnology
PublicationLignin modifying enzymes (LMEs) have gained widespread recognition in depolymerization of lignin polymers by oxidative cleavage. LMEs are a robust class of biocatalysts that include lignin peroxidase (LiP), manganese peroxidase (MnP), versatile peroxidase (VP), laccase (LAC), and dye-decolorizing peroxidase (DyP). Members of the LMEs family act on phenolic, non-phenolic substrates and have been widely researched for valorization...
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The Role of Electrostatics in Enzymes: Do Biomolecular Force Fields Reflect Protein Electric Fields?
PublicationPreorganization of large, directionally oriented, electric fields inside protein active sites has been proposed as a crucial contributor to catalytic mechanism in many enzymes, and it may be efficiently investigated at the atomistic level with molecular dynamics simulations. Here, we evaluate the ability of the AMOEBA polarizable force field, as well as the additive Amber ff14SB and Charmm C36m models, to describe the electric...
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Glucosamine-6-phosphate synthase - the multi-facets enzyme
PublicationAmidotransferaza L-glutamina: D-frukozo-6-fosforan, nazywana zwyczajowo syntazą glukozamino-6-fosforanu, jako jedyny enzym należący do grupy amidotransferaz, nie wykazuje aktywności amoniakozależnej. Enzym ten, katalizujący pierwszy wyjątkowy etap w szlaku metabolicznym prowadzącym do ostatecznego wytworzenia 5´difosfourydyno-N-acetylo-D-glukozaminy (UDG-GlcNAc) stanowi ważny punkt kontroli metabolicznej biosyntezy makromolekuł...
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Enzyme-conjugated MXene nanocomposites for biosensing and biocatalysis acuities
PublicationEngineered two-dimensional (2-D) MXenes-based materials with tunable characteristics and multi-functionalities have brought up new paradigms in the biosensing and catalysis of chemical compounds. The profusion of electroactive functional moieties on the surface of few/multi-layer MXenes facilitates their ability to retain biomolecules such as enzymes resulting in unique dimensions for bioanalytical and biosensing applications....
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Melatonin stimulates the activity of protective antioxidative enzymes in myocardial cells of rats in the course of doxorubicin intoxication
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Genistein inhibits activities of methylenetetrahydrofolate reductase and lactate dehydrogenase, enzymes which use NADH as a substrate
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Antioxidative enzymes and expression of rbcL gene as tools to monitor heavy metal-related stress in plants
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Antioxidant Potential of Curcumin—A Meta-Analysis of Randomized Clinical Trials
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Composition of polyphenols of asparagus spears (Asparagus officinalis) and their antioxidant potential
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Bioactive compounds and the antioxidant capacity in new kiwi fruit cultivars
PublicationThe aim of this investigation was to find the best among seven different kiwi fruit cultivars (‘Hayward’, ‘Daheung’, ‘Haenam’, ‘Bidan’, ‘Hort16A’, ‘Hwamei’ and ‘SKK12’) for human consumption and to classify them as groups. Therefore, the contents of bioactive compounds and the level of antioxidant capacities of these cultivars were determined in four different extracts and compared. It was found that the contents of the bioactive...
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Anticariogenic effects of extracts and fractions of Potentilla recta and their antioxidant activity
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Synthesis, physical and chemical properties of polyetherurethanes modified by natural antioxidant
PublicationOtrzymano nowe polieterouretany modyfikowane witaminą E. Ten naturalny przeciwutleniacz związany został z makrocząsteczkami w postaci bocznych odgałęzień poprzez wiązanie eterowe lub uretanowe, zależnie od użytego w syntezie jednego z dwóch rodzajów otrzymanych uprzednio monomerów. Polimery scharakteryzowano mierząc wytrzymałość na rozciąganie i twardość oraz absorpcję wody i oleju słonecznikowego. Odporność chemiczną badano oznaczając...
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Metabolomic and antioxidant properties of different varieties and origins of Dragon fruit
PublicationDragon fruit has appealed much concern from consumers as a novelty fruit with potent nutritional and medicinal benefits. Dragon fruit quality warrants comprehensive evaluation, based on the contents of pigments and health- promoting natural compounds in different varieties. This study was aimed to evaluate the differences among dragon fruit varieties extracted with methanol–water (CD3OD-D2O) and methanol (CD3OD) by proton nuclear magnetic...
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Antioxidants as a Potential Preventive and Therapeutic Strategy for Cadmium
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Computer-assisted enzyme engineering by modification of tunnels, channels and gates
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Influence of dipalmitoylphosphatidylcholine (or dioleoylphosphatidylcholine) and phospholipase A2 enzyme on the properties of emulsions
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Insect cuticular compounds affect Conidiobolus coronatus (Entomopthorales) sporulation and the activity of enzymes involved in fungal infection
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Modulation of the expression of enzymes metabolizing xenobiotics in rat liver and kidney by oral administration of cabbage and sauerkraut juice.
PublicationObydwa soki z kapusty mogą wpływać selektywnie na ekspresję genów zaangażowanych w aktywację i detoksykację kancerogenów. Efekt ten zależy od sposobu traktowania i może zależeć od rodzaju tkanki.
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Lifespan of etoposide-treated human neutrophils is affected by antioxidant ability of quercetin
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The effects of NP-POL, the nonapeptide constituent of Colostrinin, on intracellular antioxidant system
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Effect of Thermal Processing on Antioxidant Activity and Cytotoxicity of Waste Potato Juice
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Mineral Composition and Antioxidant Potential of Coffee Beverages Depending on the Brewing Method
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The role of the enzymatic antioxidant system in cylindrospermopsin-induced toxicity in human lymphocytes
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Antioxidant capacity and cholinesterase inhibiting properties of dietary infusions with Humulus lupulus
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Effect of Storage Conditions on Storability and Antioxidant Potential of Pears cv. ‘Conference’
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Antioxidant and antimicrobial activity of fermented beverages obtained from fruit pomace
PublicationFruit pomace should not be considered as a waste product but as a by-product as it contains a lot of valuable components such as dietary fiber, bioactive compounds and a source of nutrients. The reasonable way to utilize these by-products, both fresh or dried, could be the fermentation of its extracts, leading to beverages with functional properties. In our research, we checked the possibility of using chokeberry, apple and...
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Determination of Flavonoids in Selected Scleranthus Species and Their Anti-Collagenase and Antioxidant Potential
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Supplementation of garlic lowers lipids and increases antioxidant capacity in plasma of rats
PublicationZbadano bioaktywność surowego i gotowanego czosnku (Allium sativium L.). Stwierdzono, że gotowanie czosnku w temperaturze 100 °C przez 20 minut nie wpływa na jego bioaktywność.Samcom szczurów (z badanej populacji) podzielonym losowo na 10 grup, podawano żywność z różnym dodatkiem czosnku. Natomiast grupa kontrolna była karmiona dietą podstawową złożoną ze skrobi pszenicznej, oleju sojowego, celulozy, minerałów i mieszaniny witamin....
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The wetting and interfacial properties of alumina surface treated with dipalmitoylphosphatidylcholine and lipase enzyme
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Quinone- and nitroreductase reactions of Thermotoga maritima peroxiredoxin–nitroreductase hybrid enzyme
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Modelling of Thyroid Peroxidase Reveals Insights into Its Enzyme Function and Autoantigenicity
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Enzyme Conjugation - A Promising Tool for Bio-catalytic and Biotransformation Applications – A Review
PublicationEnzymes have revolutionized conventional industrial catalysts as more efficient, eco-friendly, and sustainable substitutes that can be used in different biotechnological processes, food, and pharmaceutical industries. Yet, the enzymes from nature are engineered to make them adapt and enhance their durability in the industrial environment. One promising approach involves the combined use of multiple enzymes that catalyze highly...
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Effect of furfural on the enzyme activity during enzymatic hydrolysis of cellulose isolated from poplar wood (Populus sp.) Wpływ furfuralu na aktywność enzymu podczas hydrolizy celulozy wyodrębnionej z drewna topoli (Populus sp.)
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Diclofenac Degradation—Enzymes, Genetic Background and Cellular Alterations Triggered in Diclofenac-Metabolizing Strain Pseudomonas moorei KB4
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Electrochemistry meets enzymes: Investigation of the biotransformation pathway of C-1311 based on electrochemical simulation in comparison to in vitro methods.
PublicationThe knowledge of the metabolic pathways and the biotransformation of new drugs is one of the major challenges in pharmaceutical research. It is crucial for elucidation of degradation routes of the new biologically active compounds, especially in the area of possible toxicity. Conventional in vitro drug metabolism studies are based on incubating drug candidate with e.g. hepatocytes or, most importantly, liver cell microsomes and...
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Otrzymywanie i niektóre zastosowania unieruchomionych enzymów
PublicationWyjaśniono, dlaczego immobilizacja enzymów ma duże znaczenie w przemyśle. W artykule przedstawiono sposoby immobilizacji enzymów, jak też rodzaje i właściwości stosowanych nośników. Podano także charakterystykę rozmaitych preparatów unieruchomionych enzymów, przyczyny zmian właściwości biokatalizatorów spowodowane immibilizacją oraz określono najważniejsze zastosowania wymienionych preparatów.
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Antioxidant Activities and Volatile Flavor Components of Selected Single-Origin and Blend Chocolates
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Edible Flowers Extracts as a Source of Bioactive Compounds with Antioxidant Properties—In Vitro Studies
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Application for novel electrochemical screening of antioxidant potential and phytochemicals in Cornus mas extracts
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Antioxidant properties and resveratrol content of Polish Regent wines from Podkarpacie region
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New Chalcone Derivatives Containing 2,4-Dichlorobenzenesulfonamide Moiety with Anticancer and Antioxidant Properties
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Antioxidant effect of Elamipretide on bull's sperm cells during freezing/thawing process
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Organic and conventional kiwifruit myths versus reality: antioxidant, antiproliferative and health effects
PublicationComparison between organic and conventional kiwifruit cultivars ''Hayward'' and ''Bidan'', which was done by four redical scavenging assays, ESI-MS, and DSC measurements, showed significant differences between cultivars. Such results were not estimated in kiwifruit growing under organic and conventional conditions. The extraction of bioactive compounds was done by two different methods: sequential extraction with ethyl acetate...
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A Comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk
PublicationCałkowitą zdolność przeciwutleniająca (TAC) mleka ludzkiego odzwierciedla zawartość i aktywność w mleku składników, które zapobiegają degradacji tłuszczów i białek na drodze utlenienia. Celem badania było porównuje testu ABTS i DPPH w odniesieniu do odzysku, precyzji i czułości (granica wykrywalności i oznaczalności) obu metod służących do oznaczania wartości TAC mleka ludzkiego. Wartości TAC zostały określone dla dwudziestu pięciu...