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Wyniki wyszukiwania dla: CELL WALL
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Across-shift changes in upper airways after exposure to bacterial cell wall components
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Changes in the Cell Wall Proteome of Leaves in Response to High Temperature Stress in Brachypodium distachyon
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Cell Wall Epitopes and Endoploidy as Reporters of Embryogenic Potential in Brachypodium Distachyon Callus Culture
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PAD4, LSD1 and EDS1 regulate drought tolerance, plant biomass production, and cell wall properties
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Characterization of the interactions between human high-molecular-mass kininogen and cell wall proteins of pathogenic yeasts, Candida tropicalis
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Comparison of mass spectrometry data and bioinformatics predictions to assess the bona fide localization of proteins identified in cell wall proteomics studies
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Two small RNAs conserved in Enterobacteriaceae provide intrinsic resistance to antibiotics targeting the cell wall biosynthesis enzyme glucosamine-6-phosphate synthase
PublikacjaFormation of glucosamine-6-phosphate GlcN6P) by enzyme GlcN6P synthase (GlmS) represents the first step in bacterial cell envelope synthesis. In Escherichia coli, expression of glmS is controlled by small RNAs (sRNAs) GlmY and GlmZ. GlmZ activates the glmS mRNA by base-pairing. When not required, GlmZ is bound by adapter protein RapZ and recruited to cleavage by RNase E inactivating the sRNA. The homologous sRNA GlmY activates...
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Fluconazole resistant Candida auris clinical isolates have increased levels of cell wall chitin and increased susceptibility to a glucosamine-6-phosphate synthase inhibitor
PublikacjaIn 2009 Candida auris was first isolated as fungal pathogen of human disease from ear canal of a patient in Japan. In less than a decade, this pathogen has rapidly spread around the world and has now become a major health challenge that is of particular concern because many strains are resistant to multiple class of antifungal drugs. The lack of available antifungals and rapid increase of this fungal pathogen provides an incentive...
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First isolation of polysaccharidic ulvans from the cell walls of freshwater algae
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Fibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeasts
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The Proposition of an Automated Honing Cell with Advanced Monitoring
PublikacjaHoning of holes allows for small shape deviation and a low value of a roughness profile parameter, e.g., Ra parameter. The honing process heats the workpiece and raises its temperature. The increase in temperature causes thermal deformations of the honed holes. The article proposes the construction of a honing cell, containing in addition to CNC honing machine: thermographic camera, sound intensity meter, and software for collecting...
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Induced-fit binding of laccase to gold and carbon electrodes for the biological fuel cell applications
PublikacjaAnalogues of laccase natural substrates (syringic, veratric, ferulic, vanillic, isovanillic, 3,5-dimethoxybenzoic aldehydes) were employed to bind and orient laccase molecules in a way which facilitates adsorption of the catalyst molecules and their electrical connection with the conductive support. Laccase was bound efficiently to these substrates both on gold and carbon electrodes forming, respectively, 2D and 3D films sensitive...
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Synthesis of phosphono and phosphate derivatives of hydroxyimino-D-alditols as new potential antifungal agents
PublikacjaIn search for new effective we antyfungals we focus on two enzymes involved in biosynthesis of the fungal cell wall. The first enzyme is glucosamine-6-phosphate (GlmS), which catalyzes transformation of D-fructose-6-phosphate (Fru-6P) to D-glucosamine-6-phodphate (GlcN-6P) in the chitin biosynthesis pathway. The second enzyme is phosphomannose isomerase (PMI) repored to play a crucial role in biosynthesis of many mannosylated structures,...
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Fluoroaromatic substituents attached to carbon nanotubes help to increase oxygen concentration on biocathode in biosensors and biofuel cells
PublikacjaBased on the known ability of perfluorodecalin/perfluorohydrocarbons to enhance oxygen solubility we modified oxygen sensitive biocathode by adding perfluorinated components to the catholite. This procedure improved the efficiency of the oxygen sensitive cathodes. Glassy carbon electrodes covered with single-wall carbon nanotubes (SWCNTs) with covalently bonded perfluoroaromatic groups were shown to be more sensitive to oxygen,...
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New conjugates of tuftsin and muramyl dipeptide as stimulators of human monocytes-derived dendritic cells
PublikacjaMuramyl dipeptide (MDP) and tuftsin are known biologically active compound displaying a significant influence on various cell populations of innate immune response. MDP, as a fragment of bacterial cell wall, stimulates not only macrophages and monocytes, but also dendritic cells. In contrast, little is known about tuftsin influence on these cells. Therefore it seemed vital to access whether tuftsin or its derivatives conjugated...
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Investigation of the Antifungal Activity and Mode of Action of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimum basilicum, and Eugenia caryophyllus Essential Oils
PublikacjaThe antimicrobial activity of plant oils and extracts has been recognized for many years. In this study the activity of Thymus vulgaris, Citrus limonum, Pelargonium graveolens, Cinnamomum cassia, Ocimumbasilicum, and Eugenia caryophyllus essential oils (EOs) distributed by Pollena Aroma (Nowy Dwór Mazowiecki, Poland) was investigated against a group of 183 clinical isolates of C. albicans and 76 isolates of C. glabrata. All of...
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Isolation of the GFA1 gene encoding glucosamine-6-phosphate synthase of Sporothrix schenckii and its expression in Saccharomyces cerevisiae
PublikacjaGlucosamine-6-phosphate synthase (GlcN-6-P synthase) is an essential enzyme involved in cell wall biogenesis that has been proposed as a strategic target for antifungal chemotherapy. Here we describe the cloning and functional characterization of Sporothrix schenckii GFA1 gene which was isolated from a genomic library of the fungus. The gene encodes a predicted protein of 708 amino acids that is homologous to GlcN-6-P synthases...
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Impact of the Alkyl Side Chains of Cations and Anions on the Activity and Renaturation of Lysozyme: A Systematic Study Performed Using Six Amino‐Acid‐Based Ionic Liquids
PublikacjaThe impact of the structure of ionic liquid on the activity and renaturation of lysozyme from the hen egg white (HEWL) was investigated. A set of six ionic liquids based on morpholinium cation and N-acylated amino acids as anions were exploited. The matrix used was based on two homologous series: one with N-acetyl-L-valinate [Val] as a common anion and morpholinium cation with a different number of carbon atoms in the n-alkyl chain...
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Molecular Targets for Anticandidal Chemotherapy
PublikacjaA relatively small number of anticandidal chemotherapeutics used in clinical practice is at least in part consequence of a limited number of their molecular targets: ergosterol in the membrane, lanosterol demethylase, b(1!3) glucan synthase, and DNA/RNA biosynthesis. Much more potential novel targets have been revealed by the comparative genomic studies identifying essential genes unique for Candida albicans or resulted from recognition...
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Preparation, characterization and anti-colitis activity of curcumin-asafoetida complex encapsulated in turmeric nanofiber
PublikacjaUlcerative colitis (UC) is a main form of inflammatory bowel disease (IBD). Asafoetida (ASF) and turmeric have traditionally been used for the treatment of various inflammatory diseases, including UC, because ASF is rich in sulfur compounds and turmeric contains curcumin (CUR). Turmeric nanofiber (TNF), the modified cell wall component of turmeric is considered to play important role in the human diet, health and can be used as...
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Chitosan, Collagen and Gelatin in the design of novel materials with antimicrobial acticity
PublikacjaMedical devices containing and releasing antiinfective agents directly on the site of injury are a common group of materials available and widely used in treating difficult to heal wounds. Sustained contact of the active agent to the damaged tissue, needed to inhibit infection and preservation of microbiological purity of the wound, is provided by the immobilization antimicrobial agents on the surface of the matrix material or...
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Impact of the Alkyl Side Chains of Cations and Anions on the Activity and Renaturation of Lysozyme: A Systematic Study Performed Using Six Amino‐Acid‐Based Ionic Liquids
PublikacjaThe impact of the structure of ionic liquid on the activity and renaturation of lysozyme from the hen egg white (HEWL) was investigated. A set of six ionic liquids based on morpholinium cation and N-acylated amino acids as anions were exploited. The matrix used was based on two homologous series: one with N-acetyl-L-valinate [Val] as a common anion and morpholinium cation with a different number of...
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Characterization of immobilized Escherichia coli cells transformed by β-galactosidase gene from Pyrococcus woesei
PublikacjaThermostable β-galactosidase from Escherichia coli transformant containing theenzyme gene from Pyrococcus woesei was immobilized on alginate gel.The benefits of using whole bacterial cells not only exclude expensive, laborious proteinisolation and purification but also stabilize enzymes by cytosol components. Increase inproductivity of enzyme can be achieved by cells permeabilization. To increase permeability ofcytoplasmic membrane...
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Peptidoglycan hydrolases - potential weapons against Staphylococcus aureus
PublikacjaBacteria of the genus Staphylococcus are common pathogens responsible for a broad spectrum of human and animal infections and belong to most important etiological factors causing food poisoning. Because of rapid increase in prevalence of isolation of staphylococci resistant to many antibiotics, there is an urgent need for development of new alternative chemotherapeutics. A number of studies have recently demonstrated the strong...
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Postbiotics in oncology: science or science fiction?
PublikacjaThe gut microbiome has been increasingly understood to play a critical role in carcinogenesis and cancer disease progression. The most recent research advancements have shown that different tools of microbiota manipulation contribute to gut microbiome–immune–oncology axis modulation, offering exciting opportunities for targeted interventions aimed at improving the efficacy of established anti-cancer therapy. Postbiotics are a new...
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Ultrastructural variability of macrophages in the wall of selected aorto-coronary bypass grafts
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Evaluation of adhesion strength, corrosion, and biological properties of the MWCNT/TiO2 coating intended for medical applications
PublikacjaMulti-wall carbon nanotube (MWCNT) coatings are gaining increasing interest because of their special properties used in many science fields. The titania coatings are known for their improvement of osteoblast adhesion, thus changing the surface architecture. Bi-layer coatings comprising 0.25 wt% of the MWCNTs and 0.30 wt% of titania (anatase structure) were synthesized in a two-stage procedure using the electrophoretic deposition...
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Co-simulation strategy of transient CFD and heat transfer in building thermal envelope based on calibrated heat transfer coefficients
PublikacjaThe paper presents a strategy to develop a fully transient numerical model of a heat transfer in building, using a transient CFD model of indoor air and a thermal envelope model, running in a co-simulation. The strategy relies on the utilization of RANS with a high-Re turbulence model and a wall function, which enables to use a coarse computational mesh limiting the computational time. Since wall functions give invalid heat transfer...
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Improving Thermal Insulation Properties for Prefabricated Wall Components Made of Lightweight Aggregate Concrete with Open Structure
PublikacjaPorous concrete is commonly used in civil engineering due to its good thermal insulation properties in comparison with normal concrete and high compression strength in comparison with other building materials. An extremely porous concrete, called ‘lightweight aggregate concrete with open structure’ (LAC), is used in some German plants to produce prefabricated wall components. They are used mainly in hall buildings, e.g. supermarkets....
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A novel in vivo approach to assess strains of the human abdominal wall under known intraabdominal pressure
PublikacjaThe study concerns mechanical behaviour of a living human abdominal wall. A better mechanical understanding of a human abdominal wall and recognition of its material properties is required to find mechanically compatible surgical meshes to significantly improve the treatment of ventral hernias. A non-invasive methodology, based on in vivo optical measurements is proposed to determine strains of abdominal wall corresponding to...
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Static and dynamic modelling blow- out type trauma of orbital wall
PublikacjaAuthors of the paper present initial results of finite element analysis of a blow-out type trauma of orbital wall. The research is liked with laboratory tests for the Young’s modulus of bones evaluation. In the finite element analysis the neighbourhood of orbital wall is modelled by triangle thin shell finite elements. In the paper results of nonlinear static and transient dynamic analysis (including damping) are compared. The...
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Analysis of Historic Brick Walls' Strengthening Methods
PublikacjaAnalysis of the behavior of historical brick walls is difficult and complicated. However, computer calculations are being increasingly used in the evaluation of cultural heritage buildings and some of their elements. The aim of this paper is to analyze the historic brick walls reinforcement. Different methods of strengthening are compared and discussed. Numerical analysis, based on finite element method, was conducted on the assumption...
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Micro-modelling of shear localization during quasi-static confined granular flow in silos using DEM
PublikacjaThe paper deals with the quasi-static confined flow of cohesionless sand in a plane strain model silo with parallel walls and a slowly movable bottom. Numerical modelling was carried out by the discrete element method (DEM) using spheres with contact moments to approximately capture a non-uniform shape of sand particles. Different initial void ratios of sand and silo wall roughness grades were employed. Regular triangular grooves...
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Comparative tests of the wall thickness effect of a glass-reinforced thermosetting plastic coating with a non-circular cross section in a soil-coating object for economic aspect
PublikacjaCulvert structures made of plastics are employed extensively in the construction of new facilities as well as the renovation of existing ones. Due to the high costs of materials required for the manufacturing of these goods, the objective of this research is focused to lessen the thickness of the walls, which may, on the other hand, result in a reduction in the stiffness of the system. In the course of the testing, the structure...
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Posttraumatic Orbital Emphysema: A Numerical Model
PublikacjaOrbital emphysema is a common symptom accompanying orbital fracture. The pathomechanism is still not recognized and the usually assumed cause, elevated pressure in the upper airways connected with sneezing or coughing, does not always contribute to the occurrence of this type of fracture. Observations based on the finite model (simulating blowout type fracture) of the deformations of the inferior orbital wall after a strike in...
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Self-organising maps in the analysis of strains of human abdominal wall to identify areas of similar mechanical behaviour.
PublikacjaThe study refers to the application of a type of artificial neural network called the Self-Organising Map (SOM) for the identification of areas of the human abdominal wall that behave in a similar mechanical way. The research is based on data acquired during in vivo tests using the digital image correlation technique (DIC). The mechanical behaviour of the human abdominal wall is analysed during changing intra-abdominal pressure....
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Modelling of shear zones during quasi-static granular silo flow using material point method (MPM)
PublikacjaThe paper focuses on confined silo flow of cohesionless sand. The problem considered is a quasi-static flow in a plane strain model silo with parallel walls simulated with the material point method (MPM). The simulation used a non-local hypoplastic constitutive model. Initially, the paper validated the implemented numerical approach with basic element tests and a plane strain compression test. The subsequent MPM calculations for...
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Mechanical compatibility of implants used in hernia repair with abdominal wall
PublikacjaThe paper deals with a membrane model of a synthetic surgical mesh for treatment of abdominal hernia. The authors analyse the compatibility of two implant types with the human wall: Dualmesh Gore and Proceed. The finite element method is applied to simulate tjhe behavior of the proposed model. Due fact that recurrences are usually caused by connection failure, this study is focused on reaction forces in supports representing the...
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Membrane model of human abdominal wall. Simulations vs. in vivo measurements
PublikacjaThe study presents a methodology of defining a numerical model of human abdominal wall based on the experimentally registered data of the abdomen geometry due to variations of the intraabdominal pressure. The abdominal wall is modelled here as a simple homogeneous membrane structure made of linear orthotropic material The displacements registered during the increase of pressure are compared with the re-sults of the model static...
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Wpływ strat mechanicznych w siłowniku teleskopowym zespołu zagęszczającego w pojeździe do usuwania odpadów z pojemników na skuteczność prasowania odpadów
PublikacjaHydraulic telescopic cylinders are used to power the moving wall of rear loader garbage trucks equipped with the traditional "sweep and slide" system. As the garbage compression force is directly dependent on the resistance force of the moving wall, losses in the hydraulic telescopic cylinder influence the compaction process. Taking them into account in the control system may improve the operation of the compaction...
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NUMERICAL SIMULATIONS OF HONING PROCESS OF THIN WALL CYLINDER LINERS, WITH CONSTANT AND WITH VARIABLE THICKNESS OF WALL OF HONED PARTS
PublikacjaNumerical simulations of honing process of thin-wall cylinder liners, with constant and with variable thickness of the wall of honed workpieces can improve and can help to conduct the experimental research of honing process and can improve the honing process. A very valuable research assumption, before performing the numerical analysis of computer simulation of honing process, is the measurement of the real geometry of the honing...
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Full-field in vivo experimental study of the strains of a breathing human abdominal wall with intra-abdominal pressure variation
PublikacjaThe presented study aims to assess the mechanical behaviour of the anterior abdominal wall based on an in vivo experiment on humans. Full-field measurement of abdominal wall displacement during changes of intra-abdominal pressure is performed using a digital image correlation (DIC) system. Continuous measurement in time enables the observation of changes in the strain field during breathing. The understanding of the mechanical...
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Global sensitivity analysis of membrane model of abdominal wall with surgical mesh
PublikacjaThe paper addresses the issue of ventral hernia repair. Finite Element simulations can be helpful in the optimization of hernia parameters. A membrane abdominal wall model is proposed in two variants: a healthy one and including hernia defect repaired by implant. The models include many uncertainties, e.g. due to variability of abdominal wall, intraabdominal pressure value etc. Measuring mechanical properties with high accuracy...
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Insights Into Hydrodynamics of Spinning Fluids Reactor
PublikacjaSpinning fluids reactor (SFR) is one of the most efficient gas-liquid contacting system with high potential of application in many fields. The unique solution of SFR is the contacting of two spinning fluids which generated centrifugal force. The aim of this study was to investigate the pressure field distribution on the inner surface of reactors dispersing element. Obtained results confirm the high heterogeneity of the field pressure...
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Three-dimensional printed trachea helps to design tailored treatment for tracheobronchomalacia
PublikacjaTracheobronchomalacia is a rare respiratory disease that is manifesting by impaired ventilation with expiratory collapse of the tracheal wall due to softening of the supporting cartilage and hypotonia of myoelastic elements [1]. Surgery is the mainstay of treatment. We report the case of 39 -year old man with exacerbation of chronic respiratory distress. The membranous wall of the trachea and the large bronchi was stretched to...
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CONSTRUCTION TECHNOLOGY OF TIMBER-FRAME HOUSES RESISTANT TO DYNAMIC LOADS – STUDY ON MODELS OF EXTERIOR WALLS
PublikacjaThe aim of this paper is to show the numeric representation of experimental studies concerning the behaviour of exterior wall models of a timber-frame house under harmonic loading. A single wall model according to traditional technology of timberframe house walls (filling with mineral wool) was tested. The analysis was conducted for the following frequencies: 0.5 Hz, 1.0 Hz, 2.0 Hz and 5.0 Hz for various values of the specified...
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Biomechanics of the front abdominal wall as a potential factorleading to recurrence with laparoscopic ventral hernia repair
PublikacjaThis study investigated the front abdominal wallto describe its elasticity in vivo and searched for elongationsthat possibly stretched an implanted mesh, therebycausing fixation failure and subsequent recurrence.To measure front abdominal wall elongations, amodel of fascia movements was created. Eight healthyvolunteers were measured during exercise to determine theextent of elongations in their front abdominal wall. Videoswere...
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Comparison of Properties of the Hybrid and Bilayer MWCNTs—Hydroxyapatite Coatings on Ti Alloy
PublikacjaCarbon nanotubes are proposed for reinforcement of the hydroxyapatite coatings to improve their adhesion, resistance to mechanical loads, biocompatibility, bioactivity, corrosion resistance, and antibacterial protection. So far, research has shown that all these properties are highly susceptible to the composition and microstructure of coatings. The present research is aimed at studies of multi-wall carbon nanotubes in three different...
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Full scale measurements of pressure field induced on the quay wall by bow thrusters – indirect method for seabed velocities monitoring
PublikacjaThe paper presents the results of full-scale experimental investigation of loads generated on the quay wall by bow thrusters during unberthing of a self-manoeuvring vessel. The presented research allowed for the comparison of the measurements results with generally accepted empirical prediction methods and confirmed the utility of the developed measuring setup for the on-line monitoring of jet induced loads. The conclusions from the...
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Comparative 3D DEM simulations of sand–structure interfaces with similarly shaped clumps versus spheres with contact moments.
PublikacjaThree-dimensional simulations of a monotonic quasi-static interface behaviour between initially dense cohesionless sand and a rigid wall of different roughness during tests in a parallelly guided direct shear test under constant normal stress are presented. Numerical modelling was carried out by the discrete element method (DEM) using clumps in the form of convex non-symmetric irregularly shaped grains. The clumps had an aspect...