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Wyniki wyszukiwania dla: DNA OXIDATIVE DAMAGE
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Effect of osmolytes of different type on DNA behavior in aqueous solution. Experimental and theoretical studies
PublikacjaOsmolytes, the small organic molecules accumulated in cells under environmental stress, can modulate the stability of biopolymers such as proteins and DNA. In spite of many years of research, there is no established molecular mechanism of the influence of osmolytes on DNA structure. Here, we used two model osmolytes that denature (urea) or stabilize (trimethylglycine, TMG) proteins to study their effect on DNA in aqueous solutions...
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Obrazy akustyczne dna morskiego w wybranych akwenach Bałtyku
PublikacjaMetody akustyczne obserwacji obszarów podwodnych mórz i oceanów stosowane są od początku XX wieku. Początkowo były to echosondy nawigacyjne najczęściej składające się z pojedynczych przetworników. Następnie zaczęto stosować sonary do wykrywania obiektów stanowiących przeszkody nawigacyjne pod woda, które miały głównie zastosowanie w marynarce wojennej. W drugiej połowie XX wieku rozpoczęto konstruowanie urządzeń akustycznych, których...
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Gaining knowledge through experience: developing decisional DNA applications in robotics
PublikacjaOmówiono nowatorskie podejscie do zastosowania wiedzy opartej na doświadczeniu i budowie decyzyjnego DNA w obszarach związanych z robotyką.In this article, we explore an approach that integrates Decisional DNA, a domain-independent, flexible, and standard knowledge representation structure, with robots in order to test the usability and suitability of this novel knowledge representation structure. Core issues in using this Decisional...
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Modyfikacje polimeraz DNA jako rozwiązania problemów w reakcjach PCR
PublikacjaReakcja PCR już od wielu lat jest podstawowym narzędziem stosowanym w biotechnologii molekularnej. Nadal napotykamy jednak liczne problemy podczas jej przeprowadzania związane z trudnymi matrycami, obecnością inhibitorów itp. Optymalizacja reakcji PCR jest więc niezbędna w celu poprawy wyników. Jednym z rozwiązań tych problemów może być modyfikowanie polimeraz DNA. W powyższym artykule opisano problemy i rozwiązania zależne polimerazy...
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The influence of the substrate temperature on the preparation of DNA films for studies under vacuum conditions
PublikacjaExperiments were carried out to determine the dependence of the physical form of supercoiled DNA films on the initial temperature of the substrate. Such films are often used in irradiation experiments involving low energy particles, like electrons or photons. In order to obtain absolute values for cross sections for such experiments, the spatial distribution of the sample in the film has to be well estimated. These investigations...
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The effect of elevated temperature on LCF damage growth in 2024AA – Experiment and modeling
PublikacjaThis paper presents the results of experimental low-cycle fatigue (LCF) tests conducted on EN-AW 2024 alloy in T3511 temper at 100 ◦C, 200 ◦C and 300 ◦C. The material’s basic fatigue characteristics were determined, such as fatigue life curve and cyclic strain curve. Both the hysteresis loop and nature of fracture surfaces obtained in macro- and microscopic observations (scanning electron microscope, SEM) were accounted for. The...
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Experimental verification and comparison of mode shape-based damage detection methods
PublikacjaThis paper presents experimental verification and comparison of damage detection methods based on changes in mode shapes such as: mode shape curvature (MSC), modal assurance criterion (MAC), strain energy (SE), modified Laplacian operator (MLO), generalized fractal dimension (GFD) and Wavelet Transform (WT).
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Quantitative fluorescent determination of DNA – Ochratoxin a interactions supported by nitrogen-vacancy rich nanodiamonds
PublikacjaOchratoxin A (OTA) is a hazardous contaminant of a large variety of plant and animal originated food. Herein, we report an interaction of OTA with calf thymus DNA (ct DNA) on the nanodiamond surface. We employed multispectroscopic techniques to elucidate the binding mechanism of OTA with ct DNA. The fluorescence and UV–Vis spectroscopy results show that OTA binds to ds ct DNA and forms complexes. We obtained the binding constants...
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Imaging of Increasing Damage in Steel Plates Using Lamb Waves and Ultrasound Computed Tomography
PublikacjaThis paper concerns inspection of steel plates, with particular emphasis on the assessment of in-creasing damage. Non-destructive tests were performed on four plates, one of which was un-damaged, while the remaining three had a defects in the form of circular holes with a diameter of 2, 5, and 10 cm. Guided Lamb waves were used in the research, and the image reconstruction was performed using ultrasound computed tomography. The...
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Vibration-Based Investigation of a Historic Bell Tower to Understand the Occurrence of Damage
PublikacjaABSTRACT The paper presents a survey of the historic Arch-cathedral bell tower in Vilnius induced by the appearance of cracks in the structure after a new bell system installation. An attempt is undertaken to identify the reason for the crack occurrence and to check a possible crack influence on global structural behaviour. The research is based on dynamic measurements made in the tower during ambient vibrations and during vibrations...
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Impact damage in SiO2 nanoparticle enhanced epoxy – Carbon fibre composites
PublikacjaLow velocity impact behaviour of nano-SiO2 enhanced carbon fibre/epoxy composites for naval applications is reported. Epoxy resin matrix was enhanced by 1- 8 wt% SiO2 nanoparticles, based on industrial surface-modified nanosilica. Impact parameters: force, deformation, energy, damage size were recorded. The most pronounced effect was damage size decrease; e.g. ∼28% recorded by infrared thermography and X-ray computed radiography...
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Potential degradation of norfloxacin using UV-C/Fe2+/peroxides-based oxidative pathways
PublikacjaThe removal of norfloxacin (NOR), a widely used pharmaceutical and emerging water pollutant, was studied using UV-C and Fe2+ catalyzed peroxides-based oxidative processes (e.g., UV-C/Fe2+/H2O2, UV-C/Fe2+/S2O82− and UV-C/Fe2+/HSO5−) and compared with UV-C and UV-C/Fe2+. The UV-C and UV-C/Fe2+ degraded NOR to 38 and 55 %. However, use of peroxides, i.e., H2O2, S2O82−, HSO5− with UV-C and UV-C/Fe2+ promoted NOR %degradation to 75,...
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Toward Intelligent Vehicle Intrusion Detection Using the Neural Knowledge DNA
PublikacjaIn this paper, we propose a novel intrusion detection approach using past driving experience and the neural knowledge DNA for in-vehicle information system security. The neural knowledge DNA is a novel knowledge representation method designed to support discovering, storing, reusing, improving, and sharing knowledge among machines and computing systems. We examine our approach for classifying malicious vehicle control commands...
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A proposal for knowledge sharing in the e-Decisional community using Decisional DNA
PublikacjaZaproponowano model platformy wspomagającej wymianę wiedzy w społeczeństwie decyzyjnym opartym na decyzyjnym DNA.
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Smart innovation process enhancement using SOEKS and decisional DNA
PublikacjaProduct innovation always requires a foundation based on both knowledge and experience. The production and innovation process of products is very similar to the evolution process of humans. The genetic information of humans is stored in genes, chromosomes and DNA. Similarly, the information about the products can be stored in a system having virtual genes, chromosomes and decisional DNA (DDNA). The present paper proposes...
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Wave Frequency Effects on Damage Imaging in Adhesive Joints Using Lamb Waves and RMS
PublikacjaStructural adhesive joints have numerous applications in many fields of industry. The gradual deterioration of adhesive material over time causes a possibility of unexpected failure and the need for non-destructive testing of existing joints. The Lamb wave propagation method is one of the most promising techniques for the damage identification of such connections. The aim of this study was experimental and numerical research on...
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The interaction of antitumor 1-nitroacridines with DNA in cell-free systems
PublikacjaUzyskano nowe pochodne 1-nitro i 3-nitroakrydyny wykazujące zdolność do specyficznego cięcia DNA w miejscach bezzasadowych. Z użyciem oligonukleotydu z indukowanym miejscem bezzasadowym potwierdzono taką aktywność u obu otrzymanych pochodnych.
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Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria
PublikacjaSingle-stranded DNA-binding proteins (SSBs) are indispensable elements in the cells of all living organisms. SSB proteins interact with ssDNA insequence in an independent manner, preventing them from forming secondary structures and from degradation by nucleases. In this way, SSB-binding proteins participate in all processes involving ssDNA, such as replication, repair and recombination. Although there are differences in amino...
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Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria
PublikacjaSingle-stranded DNA-binding proteins (SSBs) are indispensable elements in the cells of all living organisms. SSB proteins interact with ssDNAinsequence in an independent manner, preventing them from forming secondary structures and from degradation by nucleases. In this way, SSB-binding proteins participate in all processes involving ssDNA, such as replication, repair and recombination.Although there are differences in amino acid...
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Linear viscoelastic modelling of damage-involved structural pounding during earthquakes
PublikacjaDamage-involved structural pounding during earthquakes has been recently intensively studied using different impact force models. The results of the previous studies indicate that the linear viscoelastic model is relatively simple yet accurate in modelling pounding-involved behaviour of structures during earthquakes. The only shortcoming of the model is a negative value of the pounding force occurring just before separation, which...
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Zastosowanie immobilizowanego białka MutS do wychwytywania fragmentów DNA zawierających niesparowane zasady
PublikacjaBiałko MutS wiąże DNA zawierające błędnie sparowane oraz niesparowane zasady w DNA. Podjęto szereg prób wykorzystania tego białka jako narzędzia do detekcji nieznanych mutacji lub zagęszczania frakcji DNA z niesparowaną zasadą. Dotychczas żadna z metod oparta na użyciu białka MutS nie została wdrożona powszechnie do praktyki laboratoryjnej. Głównym powodem takiego stanu rzeczy jest stosunkowo silne powinowactwo MutS do DNA w pełni...
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Spectral Finite Element Method in Condition Monitoring and Damage Detection
PublikacjaIt is well known that the dynamic behaviour of engineering structures may carry very important and crucial information that can be further used for the assessment of their condition as well as detection of any damage induced. The current interest in monitoring techniques based on the propagation of guided elastic waves requires that numerical techniques used for modelling the phenomena associated must shift into the realm of high...
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Comparison of oxidative stability of different edible oils
PublikacjaCelem prezentowanych badań było porównanie stabilności oksydacyjnej olejów roślinnych z Polski, Finlandii i Hiszpanii. Na etapie przyspieszania utleniania zastosowano promieniowanie ultrafioletowe (UV). Po naświetlaniu olejów powstałe związki lotne ekstrahowano z fazy nadpowierzchniowej za pomocą techniki mikroekstrakcji do fazy stacjonarnej (HS-SPME) i analizowano z zastosowaniem chromatografu gazowego z detektorem płomieniowo-jonizacyjnym...
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Persistent oxidative stress in colorectal carcinoma patients
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Oxidative Stress as an Important Contributor to the Pathogenesis of Psoriasis
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Damage-involved response of two colliding buildings under non-uniform earthquake loading
PublikacjaPounding between insufficiently separated buildings, which may result in considerable damage or may even lead to the total collapse of colliding structures, has been repeatedly observed during earthquakes. Earthquake-induced collisions of buildings has been intensively studied applying various structural models. It was assumed in the analyses, however, that the seismic excitation is identical for all structural supports; whereas,...
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Preparation of DNA films for studies under vacuum conditions. The influence of cations in buffer solutions
PublikacjaBadania zostały przeprowadzone w celu określenia optymalnych warunków wymaganych do przygotowania jednolitych filmów superskręconego plazmidowego DNA do wykorzystania w doświadczeniach napromieniowania w warunkach wysokiej próżni. Badania pokazują, że znaczne szkody w DNA powstają w wyniku odpompowywania filmów DNA z roztworów zawierających jedynie czysta wodę.
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Effect of printing direction on fatigue response and damage development in additive manufactured Haynes 282 nickel superalloy
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Single-stranded DNA-binding proteins (SSBs) - sources and applicationsin molecular biology
PublikacjaBiałka wiążące się do jednoniciowego DNA (SSB) odgrywają ważną rolę w replikacji, rekombinacji i naprawie DNA u bakterii, archea i eukariota. Ostatnio wzrosło zainteresowanie białkami SSB jako przydatnymi narzędziami w metodach badawczych biologii molekularnej, analitycznych i diagnostycznych. Większość znanych prokariotycznych białek SSB zbudowanych jest z czterech podjednostek (homotetramer) z jedną domeną wiążąca (OB) jednoniciowe...
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Introducing the concept of Decisional DNA-Based web content mining
PublikacjaPrzedstawiono koncepcje zastosowania struktury reprezentacji wiedzy opartej na decyzyjnym DNA w procesie wyszukiwania informacji w internecie. Dokonano analizy eksperymentow tak wspomaganego wyszukiwania oraz sformulowano wnioski na temat przyszlych badan prowadzacych do dalszej integracji procesu wyszukiwania z doswiadczeniami zdobytymi w trakcie tego wyszukiwania.
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Genome-Wide DNA Methylation and Gene Expression in Patients with Indolent Systemic Mastocytosis
PublikacjaMastocytosis is a clinically heterogenous, usually acquired disease of the mast cells with a survival time that depends on the time of onset. It ranges from skin-limited to systemic disease, including indolent and more aggressive variants. The presence of the oncogenic KIT p. D816V gene somatic mutation is a crucial element in the pathogenesis. However, further epigenetic regulation may also affect the expression of genes that...
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Sequence-dependent structural properties of B-DNA: what have we learned in 40 years?
PublikacjaThe structure of B-DNA, the physiological form of the DNA molecule, has been a central topic in biology, chemistry and physics. Far from uniform and rigid, the double helix was revealed as a flexible and structurally polymorphic molecule. Conformational changes that lead to local and global changes in the helix geometry are mediated by a complex choreography of base and backbone rearrangements affecting the ability of the B-DNA...
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Covalent DNA modification by products of myrosinase catalysed glucosinolate degradation in cell-free system
PublikacjaThe bioactive phytochemicals found in Brassica vegetables belonging to glucosinolates (GLS) and especially the products of their degradation isothiocyanates (ITC) and indoles are regarded as the most promising cancer chemopreventive compounds. These secondary metabolites constitute defence system repelling or preventing the development of agrophages attacking brassica plants. The antibiological properties of these compounds suggest...
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Defining a novel domain that provides an essential contribution to site-specific interaction of Rep protein with DNA
PublikacjaAn essential feature of replication initiation proteins is their ability to bind to DNA. In this work, we describe a new domain that contributes to a replication initiator sequence-specific interaction with DNA. Applying biochemical assays and structure prediction methods coupled with DNA–protein crosslinking, mass spectrometry, and construction and analysis of mutant proteins, we identified that the replication initiator of the...
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Set of Experience and Decisional DNA: Toward Brains for Cyber-physical Systems and Industry of the Future
PublikacjaThe concept of Decisional DNA is decade old. This article introduces the initial idea of Set of Experience Knowledge Structure, its advancement into Decisional DNA, and its potential for real life applications in divers domains. The most current and future research steps into Industry 4.0 are also presented and discussed
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Prediction based on integration of Decisional DNA and a feature selection algorithm Relief-F
PublikacjaThe paper presents prediction model based on Decisional DNA and Set of experienced integrated with Relief_F algorithm for feature selection
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Unraveling the Interplay between DNA and Proteins: A Computational Exploration of Sequence and Structure-Specific Recognition Mechanisms
PublikacjaMy PhD dissertation focused on DNA-protein interactions and the recognition of specific DNA sequences and structures. I discovered that acidic amino acid residues (Asp/Glu) play a crucial role by exhibiting a preference for cytosine. Their contribution to binding affinity depends on nearby cytosines, balancing electrostatic repulsion with specific interactions. Acidic residues act as negative selectors, discouraging non-cytosine...
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Real-time isothermal DNA amplification monitoring in picoliter volumes using an optical fiber sensor
PublikacjaRolling circle amplification (RCA) of DNA can be considered as a great alternative to the gold standard polymerase chain reaction (PCR), especially during this pandemic period, where rapid, sensitive, and reliable test results for hundreds of thousands of samples are required daily. This work presents the first research to date on direct, real-time and label-free isothermal DNA amplification monitoring using a microcavity in-line...
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Decisional DNA based intelligent knowledge model for flexible manufacturing system
PublikacjaModeling an effective mechanism for design and control strategies for the implementation of a flexible manufacturing system (FMS) has been a challenge. Consequently, to overcome this issue various techniques have applied in the past but most of these models are effective only for some specific situation or an element of FMS. In this study, the knowledge representation technique of Decisional DNA (DDNA) is applied to FMS to develop...
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A universal NDT method for examination of low energy impact damage in CFRP with the use of TLC film
PublikacjaThe article presents an attempt to use a sheet of laminated thermochromic liquid crystal film (TLC film) for non-destructive testing of the impact damage (energy values: 1J, 2J, 3J and 4J) in carbon fibre-reinforced polymer (CFRP). This is a new, alternative NDT approach based on the thermo-optical effect. The main advantages of this method are a) the low cost of TLC film, b) the low cost of recording devices due to the usage of...
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Aplikacja wspomagająca przetwarzanie sekwencji dna: moduł dopasowań
PublikacjaBiologia molekularna jest obecnie bardzo dynamicznie rozwijającą się dziedziną nauki. Wzrost mocy obliczeniowej komputerów pozwala na coraz szybszą i dokładniejszą analizę wielocząsteczkowych biologicznych polimerów. Poniższy artykuł przedstawia jeden z modułów programu AlignGator - modułowego systemu przeznaczonego do analizy DNA. Omawiany moduł pozwala na tworzenie, oraz edycję wielodopasowań. W początkowej części artykułu opisane...
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Experimental study on the effectiveness of polymer damper in damage reduction of temporary steel grandstand
PublikacjaA large number of accidents, involving collapses of temporary grandstands during different types of events, were observed in the past. If the synchronized movement of people is tuned with the natural frequency of the affected part of the structure, resonance might occur. It may lead to severe damages of grandstands, their collapse or panic among the spectators. The aim of the paper is to assess, through preliminary experimental...
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Damage Detection in the Wind Turbine Blade Using Root Mean Square and Experimental Modal Parameters
PublikacjaThe paper presents results of an experimental study related to a non-destructive diagnostic technique used for preliminary determination the location and size of delamination in composite coatings of wind turbine blades. The proposed method of damage detection is based on the analysis of the ten first mode shapes of bending vibrations, which correspond to displacements of rotor blades perpendicular to the rotor plane. Modal parameters...
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Numerical modeling of GPR field in damage detection of a reinforced concrete footbridge
PublikacjaThe paper presents a study on the use of the ground penetrating radar (GPR) method in diagnostics of a footbridge. It contains experimental investigations and numerical analyses of the electromagnetic field propagation using the finite difference time domain method (FDTD). The object of research was a reinforced concrete footbridge over a railway line. The calculations of the GPR field propagation were performed on a selected cross-section...
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Decisional DNA with embedded Relief-F linear regression for knowledge and experience management
PublikacjaOmowiono zastowania Decyzyjnego DNA, regresji liniowej oraz funkcji RELIEF-F w procesach formalnego modelowania i wspomagania zarzadzania wiedza oraz zarzadzania doswiadczeniem.
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DNA repair is responsible for the presence of oxidatively damaged DNA lesions in urine
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Selective H2 production from plastic waste through pyrolysis and in-line oxidative steam reforming
PublikacjaThis study deals with the proposal of pyrolysis and in-line oxidative steam reforming (P-OSR) for plastic waste valorization and assesses the potential of this strategy for the selective production of H2. Overall, the study aims at progressing towards the fine-tuning of the pyrolysis-reforming technology by co-feeding O2. Thus, a multi-point O2 injection system has been developed to ensure a suitable O2 distribution in the reforming...
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Low-Molecular Pyrazine-Based DNA Binders: Physicochemical and Antimicrobial Properties
PublikacjaPyrazine and its derivatives are a large group of compounds that exhibit broad biological activity, the changes of which can be easily detected by a substituent effect or a change in the functional group. The present studies combined theoretical research with the density functional theory (DFT) approach (B3LYP/6-311+G**) and experimental (potentiometric and spectrophotometric) analysis for a thorough understanding of the structure...
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The Neural Knowledge DNA Based Smart Internet of Things
PublikacjaABSTRACT The Internet of Things (IoT) has gained significant attention from industry as well as academia during the past decade. Smartness, however, remains a substantial challenge for IoT applications. Recent advances in networked sensor technologies, computing, and machine learning have made it possible for building new smart IoT applications. In this paper, we propose a novel approach: the Neural Knowledge DNA based Smart Internet...
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Decisional DNA based framework for representing Virtual Engineering Objects
PublikacjaIn this paper, we propose a frame-work to represent the Virtual Engineering Objects (VEO) utilizing Set of Knowledge Experience Structure (SOEKS) and Decisional DNA. A VEO will enable the discovery of new knowledge in a manufacturing unit and the generation of new rules that drive reasoning. The proposed VEO framework will not only be knowledge based representation but it will also have its associated experience embedded within...