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Wyniki wyszukiwania dla: METAGENOMIC APPROACH
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The metagenomic approach to characterization of the microbial community shift during the long-term cultivation of anammox-enriched granular sludge
PublikacjaA metagenomic approach was used to investigate how the microbial community composition changes when an anammox-based granular sludge reactor is seeded with nitritation-anammox biomass from a wastewater treatment plant. In the seed sample, the abundance of Candidatus Kuenenia stuttgartiensis was similar to Candidatus Jettenia caeni (12.63 vs. 11.68%). This biomass was typical in terms of microbial nitrogen conversion; both ammonia...
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Going to extremes – a metagenomic journey into the dark matter of life
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Direct cloning of heavy metal resistance genes from metagenomic DNA
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The use of fosmid metagenomic libraries in preliminary screening for various biological activities
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The Characteristics of New SSB Proteins from Metagenomic Libraries and Their Use in Biotech Applications
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Identification and molecular modeling of a novel lipase from an Antarctic soil metagenomic library.
PublikacjaIn this work, we present the construction of a metagenomic library in Escherichia coli using pUC19 vector and environmental DNA directly isolated from Antarctic topsoil and screened for lipolytic enzymes. The screening on agar supplemented with olive oil and rhodamine B revealed one clone with lipolytic activity (Lip1) out of 11,000 E. coli clones. This clone harbored a plasmid, pLip1, which has an insert of 4722 bp that has been...
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Investigation of interaction between xanthene dye rhodamine B and metagenomic-derivated methylthioadenosine phosphorylase RSFP
PublikacjaIn the previous study we found that the expression of the metagenomic - derived methylthioadenosine phosphorylase gene (rsfp) in E. coli cells and the presence of rhodamine B in the growth medium were crucial for the pink fluorescence of one E. coli colony from Antarctic soil metagenomic library (Cieśliński et al., 2009). In this study, rsfp gene expression was induced by an arabinose promoter in an LMG194 E. coli strain and the...
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A study on the interaction of rhodamine B with methylthioadenosine phosphorylase protein sourced from an Antarctic soil metagenomic library.
PublikacjaThe presented study examines the phenomenon of the fluorescence under UV light excitation (312 nm) of E. coli cells expressing a novel metagenomic-derived putative methylthioadenosine phosphorylase gene, called rsfp, grown on LB agar supplemented with a fluorescent dye rhodamine B. For this purpose, an rsfp gene was cloned and expressed in an LMG194 E. coli strain using an arabinose promoter. The resulting RSFP protein was purified...
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An examination of an Antarctic soil metagenomic-derivate putative methylthioadenosine phosphorylase gene as a novel reporter gene for promoter trapping
PublikacjaThe rsfp gene was discovered during examination ofan Antarctic soil-derived metagenome library and encodesa bacterial methylthioadenosine phosphorylase (GenBank accession number GQ202582). In a previous study, we found that E. coli colonies expressing the rsfp gene reveal strong pink fluorescence after exposure to UV light when grown on culture medium supplemented with rhodamine B (Cieśliński et al., 2009). In contrast to that,...
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Increasing conclusiveness of metabonomic studies by cheminformatic preprocessing of capillary electrophoretic data on urinary nucleoside profiles
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Calibration and metrological approach.
PublikacjaPrzedstawiono teraźniejsze podejście do procesu kalibracji. Różnorodność definicji dotyczących pomiarów w chemii analitycznej, nierzadko niekonsekwentnych, może wprowadzić w zakłopotanie. W pracy wyjaśniono pojęcia takie jak: kalibracja, substancje wykorzystywane do kalibracji, walidacja, niepewność materiałów odniesienia. Omówiono również pojęcie spójności pomiarowej w odniesieniu do procesu kalibracji.
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Calibration in Metrological Approach
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A process approach to innovation
PublikacjaOmówiono zarządzanie wiedza zawarta w innowacjach w ujęciu systemowo procesowym.
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Collaborative approach to trustworthiness of it infrastructures
PublikacjaArtykuł wprowadza koncepcję ''trust case'' i wyjaśnia jak jest ona używana w celu uzasadnienia zaufania do systemów i infrastruktur IT. W szczególności skoncentrowano się na procesie budowy trust case, wyjasniono jego strukturę oraz podkreślono konieczność włączenia do procesu właściwych udziałowców. Załączono również informację na temat funkcjonalnej struktury narzedzi wspomagających zarządzanie trust case w ramach jego cyklu...
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Contextual approach to reasoning with rules
PublikacjaIn the paper we present a method of reasoning with Horn rules within a contextual framework. We show that by proper use of a structure of contexts we can obtain partial OWA compliance with no necessity of extending the syntax of underlying Description Logics.
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An Approach to Trust Case Development
PublikacjaIn the paper we present an approach to the architectural trust case development for DRIVE, the IT infrastructure supporting the processes of drugs distribution and application. The objectives of DRIVE included safer and cheaper drugs distribution and application. A trust case represents an argument supporting the trustworthiness of the system. It is decomposed into claims that postulate some trust related properties. Claims differ...
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An approach to smart experience management
PublikacjaW pracy przedstawiono procesowe podejście do inteligentnego zarządzania wiedzą zebraną na podstawie przebytych doświadczeń.
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System of innovation: a sectoral approach
Publikacjathe article presents a systems approach to innovation as an alternative method of assessing the sector innovativeness. in the article the building blocks of each sectoral system of innovations (ssi) such as a knowledge base, institutions and actors are discussed. moreover, the five models of ssi are presented.
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An approach for evaluating trust in it infrastructures
PublikacjaW artykule zademonstrowano metodę oceny poziomu zaufania do systemu TI w oparciu o podejście funkcji przekonania.
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An MTA-phosphorylase gene discovered in the metagenomic library derived from Antarctic top soil during screening for lipolytic active clones confers strong pink fluorescence in the presence of rhodamine B.
PublikacjaIn this work, we present the construction of a metagenomic library in Escherichiacoli using the pUC19 vector and environmental DNA directly isolated fromAntarctic topsoil and screened for lipolytic enzymes. Unexpectedly, the screeningon agar supplemented with olive oil and rhodamine B revealed one unusual pinkfluorescent clone (PINKuv) out of 85 000 clones. This clone harbored a plasmid,pPINKuv, which has an insert of 8317 bp that...
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A consensus-based approach to the distributed learning
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Risk Modelling for Passages in Approach Channel
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An agent-based approach to ANN training
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An Approach to RBF Initialization with Feature Selection
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A note on the pseudoinverse approach to estimating ratios.
PublikacjaArtykuł dotyczy metody porównań parami, stosowanej w metodzie AHP w celu uszeregowania skończonej liczby czynników. Do rozwiązania problemu stosowane są głównie trzy techniki estymacji macierzy ocen za pomocą macierzy ilorazów. Autorzy stosują metodę logarytmicznych najmniejszych kwadratów, ponieważ pozwala na rozwiązanie zagadnienia z brakującymi danymi oraz pokazują pewne wybrane własności rozwiązania. Zamieszczono przykładowe...
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A new approach to the prosthetic finger design
PublikacjaW pracy zaprezentowano nowy sposób projektowania protez ręki przy użyciu aplikacji CAD. Uzyskano projekt palca wskazującego, w którym zaproponowano napęd poszczególnych członów w oparciu o stopy z pamięcią kształtu.
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Covariance approach to the free photon field
PublikacjaWprowadziliśmy teorię fotonu poprzez tą samą zasadę, co dla pojedynczej czą-stki w kwantowej mechanice używając C*-algebraicznego podejścia kowariantychukładów.
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Thermodynamical approach to quantifying quantum correlations
PublikacjaWe consider the amount of work which can be extracted from a heat bath using a bipartite state ρ shared by two parties. In general it is less then the amount of work extractable when one party is in possession of the entire state. We derive bounds for this “work deficit” and calculate it explicitly for a number of different cases. In particuar, for pure states the work deficit is exactly equal to the distillable entanglement of...
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A service-oriented approach to the identification of IT Risk
PublikacjaArtykuł przedstawia ogólne podejście do identyfikacji ryzyka eksploatacji systemów informatycznych, które obejmuje bezpieczeństwo, ochronę, prywatność oraz uzależnienie. Podejście oparte jest na badaniu trybów awarii usług dostarczanych użytkownikom przez oprogramowanie. Identyfikacja ryzyka na podstawie przeglądu usług uzupełniana jest listami kontrolnymi wyprowadzonymi ze standardów, a także klasycznymi technikami analizy bezpieczeństwa...
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A new approach to visual system testing
PublikacjaOpisano budowę laboratoryjnego stanowiska prac bawczych nad perymetrią obiektywną. Przedstawiono zasadę działania algorytmu VEPDA oraz wyniki działania VEPDA na danych eksperymentalnych.
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Determination of resonances by the optimized spectral approach
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New approach to the chunk recoginition in Polish
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A Novel Approach to the Assessment of Cough Incidence
PublikacjaIn this paper we consider the problem of identication of cough events in patients suffering from chronic respiratory diseases. The information about frequency of cough events is necessary to medical treatment. The proposed approach is based on bidirectional processing of a measured vibration signal - cough events are localized by combining the results of forward-time and backward-time analysis. The signal is at rst transformed...
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Hybrid Approach to Networked Control System
PublikacjaEffcient control of Networked Control System (NCS) is a challenge, as the control methods need to deal with non-deterministic variable delays and data loss. This paper presents a novel hybrid approach to NCS where Model Predictive Control (MPC) is applied as a main controller and implicit switching MPC is used for data transmission control in event-driven shared communication medium, leading to complex control system with active...
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A modern approach to an unmanned vehicle navigation
PublikacjaA traditional approach to manned vehicles navigation uses a data combined form a variety of navigation sensors like satellite, inertial and time-of-flight. With support of operators perception, chart and sensor, data are analyzed and navigation decisions are made. An unmanned platforms navigation needs an operators support, who is supervising a platforms decision process, basing on navigation data obtained via variety of electronic...
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Adaptive Management Approach to an Infrastructure Project
PublikacjaThe paper presents an adaptive approach to project management, illustrated by the description of a case study, i.e., of a project whose aim was to develop the means and prepare the documentation for the revitalization of a railway line. The project itself was a major challenge for the implementers due to a novel and innovative subject thereof, including the construction of a railway line which takes place in a difficult terrain...
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Informal work in Poland - a regional approach
PublikacjaIn our paper we try to combine the theories explaining the prevalence of informal work, using a regional approach. Reviewing the literature, we observe that, there is no evidence to confirm that the relationships that occur in cross-country comparisons remain significant when we use regional data and analyse cross-regional variations. Our general question is therefore whether there is simply more informal work in less-developed...
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Agile Media Management Approach – an analysis
PublikacjaThis article studies media management and demonstrates a fresh perspective on the new approach based on agile methodologies of project management. The analysis sequentially presents a literature overview of media and its management, the characteristics of media projects, agile project management, an analysis of agile approaches and media management along with the proposal of a...
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Finnish Lexical Homonimics – A Quantitative Approach
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Aggregation of imidazoacridinones - spectroscopic and microcalorimetric approach
PublikacjaImidazoacridinones (IA) belong to a group of polycyclic heteroaromatic antitumor compounds. IA create the aggregated form in water solution. Self-association of substances is a very important phenomenon in many fields of applied chemistry. It is also fundamental model reaction of many kinds of molecular interactions such as the micelle formation of amphiphilic substances and the binding of small organic molecules to macromolecules....
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An approach to determining tinnitus acoustical characteristic
PublikacjaFor many treatment methods, accurate estimation of Tinnitus(ringing in ears) concerning sound type, level, and bandwidth or frequency is inevitable. The proposed way of obtaining Tinnitus parameters is described in this paper. The method employs sound synthesis, aimed at obtaining sound which is closest to perceived Tinnitus. The proposed method assumes running a designed application on a multimedia PC provided with a special graphical...
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Systemic approach to modelling the human brain
PublikacjaW prezentacji przedstawia się ogólną koncepcję w pełni autonomicznego systemu decyzyjnego dla robota (awatara, linguabota), zdolnego reagować na bodźce ze środowiska oraz opartego na opracowanym matematycznym modelu psychologii poznawczej człowieka, z elementami psychologii osobowości., uwzględniającymi 'potrzeby', 'emocje', 'nastrój' oraz elementy 'woli'.
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Collaborative approach to WordNet and Wikipedia integration
PublikacjaIn this article we present a collaborative approach tocreating mappings between WordNet and Wikipedia. Wikipediaarticles have been first matched with WordNet synsets in anautomatic way. Then such associations have been evaluated andcomplemented in a collaborative way using a web application.We describe algorithms used for creating automatic mappingsas well as a system for their collaborative development. Theoutcome enables further...
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An approach to evaluation of arguments in trust cases
PublikacjaTrustworthiness of IT systems can be justified using the concept of a trust case. A trust case is an argument structure which encompasses justification and evidence supporting claimed properties of a system. It represents explicitly an expert's way of assessing that a certain object has certain properties. Trust cases can be developed collaboratively on the basis of evidence and justification of varying quality. They can be complex...
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Systemic approach to Road Safety Schemes
PublikacjaW artykule przedstawiono koncepcje rozwoju systemu bezpieczeństwa ruchu drogowego na poziomie krajowym oraz na poziomie regionalnym i lokalnym.
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Stochastic approach to movements of a multihull on waves
PublikacjaW artykule przedstawiono metodę wyznaczania różniczkowych równań ruchu.Układ równań różniczkowych został przedstwaiony dla ruchów antysymetrycznych jednoski wielokadłubowej (poprzeczne ruchy liniowe, poprzeczne ruchy kątowe, poziome ruchy kątowe). Dla tych ruchów zbudowano stochastyczne równania różniczkowe.
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Lexicographic Approach to Health Care Optimization
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Hybrid approach to ontology specification and development
PublikacjaIn this chapter a first draft of a hybrid ontology development approach is presented. The context of this method encompasses the multi-agent system equipped with knowledge bases and inferring engine. The process of ontology engineering appears as a complex issue, as it addresses different point-views of both, a client and a modeler with a requirements/system analyst. Regarding this, an approach based on the knowledge preprocessing...
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CONTEMPORARY APPROACH TOWARDS RESPONSIVE ARCHITECTURE
PublikacjaConstantly changing environment pressures every living for a change, and response becomes a basic reaction of any living form. Change is a natural reaction that enables survivor of all the species. However, in architecture, the responsive structure did not take important role until the twentieth century. During that time many concepts and building introduced kinematics into the discipline. In the late 50 and 60 of XX century, visionary...
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TSR method for burns investigation approach
PublikacjaThe article presents the possibilities of applying the TSR method for the diagnosis of burn wounds. The wound area is stimulated by cold gas and a sequence of thermograms of heating the tissue is recorded. Next, TSR algorithms are used to determine the parameters that allow the wound to be classified into one of the classes: the wound heals within three weeks, the wound will not heal in three weeks. The method was...