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Wyniki wyszukiwania dla: REALTIME PCR
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New Approaches for Escherichia coli Genotyping
PublikacjaEasy-to-perform, fast, and inexpensive methods of differentiation of Escherichia coli strains beyond the species level are highly required. Herein two new, original tools for genotyping of E. coli isolates are proposed. The first of the developed method, a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) test uses a highly variable fliC gene, encoding the H antigen as a molecular target. The designing...
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Investigation of Parallel Data Processing Using Hybrid High Performance CPU + GPU Systems and CUDA Streams
PublikacjaThe paper investigates parallel data processing in a hybrid CPU+GPU(s) system using multiple CUDA streams for overlapping communication and computations. This is crucial for efficient processing of data, in particular incoming data stream processing that would naturally be forwarded using multiple CUDA streams to GPUs. Performance is evaluated for various compute time to host-device communication time ratios, numbers of CUDA streams,...
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Magdalena Brzozowska-Woś dr hab. inż.
OsobyMagdalena Brzozowska-Woś jest absolwentką Wydziału Zarządzania i Ekonomii Politechniki Gdańskiej (specjalność: systemy zarządzania). Jest również absolwentką Podyplomowego Studium Reklamy (Wydział Zarządzania i Ekonomii PG) oraz Podyplomowego Studium Public Relations (SWPS Uniwersytet Humanistycznospołeczny). W latach 2000-2003 współpracowała z Panoramą Internetu sp. z o. o. jako specjalista ds. marketingu. Pełniła również obowiązki...
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Anticancer Imidazoacridinone C-1311 is Effective in Androgen-Dependent and Androgen-Independent Prostate Cancer Cells
PublikacjaAndrogen receptor (AR) plays a crutial role in prostate cancer (PCa) development and metastasis. Here, we reported potent anti-PCa activity of a small molecule imidazoacridinone C-1311. In AR-positive PCa cells, C-1311 was found to inhibit the transcriptional activity of AR uncovering a novel mechanism that may be relevant for its anticancer effect. Mechanistically, C-1311 decreased AR binding to prostate-specific antigen (PSA)...
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RAGE as a Novel Biomarker for Prostate Cancer: A Systematic Review and Meta-Analysis
PublikacjaThe receptor for advanced glycation end-products (RAGE) has been implicated in driving prostate cancer (PCa) growth, aggression, and metastasis through the fueling of chronic inflammation in the tumor microenvironment. This systematic review and meta-analysis summarizes and analyzes the current clinical and preclinical data to provide insight into the relationships among RAGE levels and PCa, cancer grade, and molecular effects....
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Processing, mechanical and thermal behavior assessments of polycaprolactone/agricultural wastes biocomposites
PublikacjaIn this paper, brewer’s spent grain (BSG) was applied as potential lignocellulose biofiller in biocompos-ites based on polycaprolactone (PCL). The PCL/BSG biocomposites filled with varying content of biofillerswere prepared via low-temperature melt-compounding. These conditions allow limiting thermal degra-dation of used biofillers during processing. The influence of biofiller content (ranging from 25 to 200parts by weight on 100...
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Modified polycarbonate urethane: synthesis, properties and biological investigation in vitro
PublikacjaNowy poliweglano uretan (PCU-I) otrzymano z alifatycznych monomerów: poliwęglanu heksanodiolu, 4,4'diizocyjanianu dicykloheksanometanu, mieszaniny małocząsteczkowych dioli i oleju rycynowego (zawierającego głównie trigliceryd kwasu 12-hydroksyoleinowego). PCU-II otrzymano analogicznie ale bez udziału oleju rycynowego. Obydwa PCU charakteryzowały się dobrą wytrzymałością na rozciąganie. PCU-I miał niższą temperaturę zeszklenia a...
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Prediction of protein assemblies, the next frontier: The CASP14‐CAPRI experiment
PublikacjaWe present the results for CAPRI Round 50, the 4th joint CASP-CAPRI protein assembly prediction challenge. The Round comprised a total of 12 targets, including 6 dimers, 3 trimers, and 3 higher-order oligomers. Four of these were easy targets, for which good structural templates were available either for the full assembly, or for the main interfaces (of the higher-order oligomers). Eight were difficult targets for which only distantly...
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Extended-spectrum beta-lactamase-producing Klebsiella pneumoniae in a neonatal unit: control of an outbreak using a new ADSRRS technique
PublikacjaSzczepy Klebsiella pneumoniae (EPKP)produkujące beta-laktamazy o rozszerzonym spektrum substratowym (ESBL)stanowią często problem zakażeń na oddziałach noworodkowych. Izolaty od 149 pacjentów poddano typowaniu nową metodą opartą na zjawisku supresji PCR, ADSRRS-fingerprinting, wykazując pochodzenie klonalne badanych szczepów. Użyteczność techniki ADSRRS-finerprinting została potwierdzona innymi metodami - RAPD i PFGE.
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Novel thermostable single-stranded DNA-binding protein (SSB) from Deinococcus geothermalis
PublikacjaW celu zbadania właściwości biochemicznych białka wiążącego się do jednoniciowego DNA (SSB) pochodzącego z Deinococcus geothermalis (DgeSSB), sklonowano gen ssb otrzymany w reakcji PCR, a następnie uzyskano wydajną biosyntezę białka DgeSSB. Gen składa się z 900 par zasad, kodujących białko złożone z 300 reszt aminokwasowych, o wyliczonej masie molekularnej równej 32.45 kDa. Sekwencja aminokwasowa wykazuje 43, 44% i 75% identyczności...
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Degradation of Polycaptolactone Modified with TPS or CaCO3 in Biotic/Abiotic Sewater
PublikacjaThe polymer degradation process in two types of water sourced from the Baltic Sea was investigated. Polycaprolactone modified with thermoplastic starch(PCL/TPS>85%) or calcium carbonate (60% PCL/40% CaCO3)and PCL alone were incubated for 28 weeks in sea water with and without microrganisms. The experimental results indicated that in each of the experiments degradation occured. The process was more effective in sea water with microorganisms.Modification...
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Thermostable Pyrococcus woesei beta-D-galactosidase - high level expression, purification and biochemical properties
PublikacjaGen kodujący termostabilną beta-D-galaktozydazę Pyrococcus woesei zamplifikowano z zastosowaniem techniki PCR i klonowano w komórkach Escherichia coli. Skonstruowany system ekspresji genu pozwolił na uzyskanie znacznej ilości termostabilnego białka w komórkach mezofilnego gospodarza. Enzym oczyszczono z zastosowaniem chromatografii powinowactwa po wcześniejszej denaturacji termicznej białek Escherichia coli i scharakteryzowano...
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Total mercury in great cormorant (Phalacrocorax carbo) and fish in polish sector of Vistula Lagoon
PublikacjaAnalizowano tkanki miękkie 55 osobników kormorana czarnego oraz wybrane gatunki ryb stanowiące ich pokarm. Badane próbki pochodziły z ekosystemu Zalewu Wiślanego. Zawartość rtęci oznaczono w narządach wewnętrznych kormorana czarnego takich jak: wątroba, nerki, mięśnie, przełyk, żołądek, jelita, tchawica, płuca, mięsień sercowy. Przebadano następujące gatunki ryb: śledź, stynka, karaś, karaś srebrzysty, płoć, lin, jazgarz. Oznaczenia...
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Development of the New Polish Method for Capacity Analysis of Motorways and Expressways
PublikacjaThe paper presents development of the new Polish method for performing capacity analysis of basic segments of dual carriageway roads (motorways and expressways). The method is based on field traffic surveys conducted at 30 motorway and expressway sites (class A and S roads) in Poland. Traffic flows, composition and travel times were observed in 15-min intervals at each site using ANPR filming method. These data were used to calibrate...
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Paweł Burdziakowski dr inż.
Osobydr inż. Paweł Burdziakowski jest specjalista w zakresie fotogrametrii i teledetekcji lotniczej niskiego pułapu, nawigacji morskiej i lotniczej. Jest również licencjonowanym instruktorem lotniczym oraz programistą. Głównymi obszarami zainteresowania jest fotogrametria cyfrowa, nawigacja platform bezzałogowych oraz systemy bezzałogowe, w tym lotnicze, nawodne, podwodne. Prowadzi badania w zakresie algorytmów i metod poprawiających...
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Development of Gas Sensor Array for Methane Reforming Process Monitoring
PublikacjaThe article presents a new method of monitoring and assessing the course of the dry methane reforming process with the use of a gas sensor array. Nine commercially available TGS chemical gas sensors were used to construct the array (seven metal oxide sensors and two electrochemical ones). Principal Component Regression (PCR) was used as a calibration method. The developed PCR models were used to determine the quantitative parameters...
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New matrix-free reference material for ethene in the form of optical fibres
PublikacjaReference materials are indispensable in the qualitycontrol and quality assurance of analytical measurements. Onenovel approach to the generation of standard gaseous mixturesof toxic, reactive, volatile, labile, and malodorous substancesinvolves thermal decomposition or rearrangement, under definedtemperature conditions, of compounds immobilized, bychemical bonding, on the surface of an appropriate carrier torelease specific amounts...
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Podstawy Sterowania Komputerowego - 2023
Kursy Online6 semestr ACR (Automatyka, Cybernetyka i Robotyka)
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Podstawy Sterowania Komputerowego - 2022
Kursy Online6 semestr ACR (Automatyka, Cybernetyka i Robotyka)
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PSK_2025
Kursy Online6 semestr ACR (Automatyka, Cybernetyka i Robotyka)
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Experimental investigations on the mechanical properties and damage detection of carbon nanotubes modified crumb rubber concrete
PublikacjaThis study presents a modified crumb rubber (MCR) concrete design mix reinforced with multi-walled carbon nanotubes (MWCNTs), mechanical characterization, and cracking monitoring using the acoustic emission (AE) technique. The results showed that the bridging effect of MWCNTs and MCR in the concrete mix mitigated the shortcomings of MWCNT-MCR concrete and improved the flexural and compressive strengths by 18.3% and 26.5%, respectively,...
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Karol Grębowski dr inż.
OsobyKarol Grębowski (dr inż.) pracuje jako adiunkt w Katedrze Technicznych Podstaw Projektowania Architektonicznego na Wydziale Architektury Politechniki Gdańskiej. Jego badania naukowe dotyczą zjawisk szybkozmiennych zachodzących podczas drgań konstrukcji budowlanych, obiektów mostowych (trzęsienia ziemi) oraz badania w zakresie metodologii projektowania budynków stanowiących system ochrony pasywnej (SOP) odpornych na uderzenia pojazdów...
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Camera sabotage detection for surveillance systems
PublikacjaCamera dysfunction detection algorithms and their utilization in realtime video surveillance systems are described. The purpose of using the proposed analysis is explained. Regarding image tampering three algorithms for focus loss, scene obstruction and camera displacement detection are implemented and presented. Features of each module are described and certain scenarios for best performance are depicted. Implemented solutions...
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Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus - expression and purification
PublikacjaBiałka SSB są niezbędne do życia każdemu organizmowi. W tej pracy prezentujemy wyniki otrzymania i oczyszczania białek SSB Thermus thermophilus i T. aquaticus. Dotąd były znane białka SSB jedynie o budowie homotetrameru lub heterotrimeru. Białka SSB Thermus thermophilus i T. aquaticus stanowią nową klasę białek SSB - białek homodimerycznych. Ponadto dowiedziono, iż białka te są użyteczne w reakcji PCR.
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A non-linear direct peridynamics plate theory
PublikacjaIn this paper a direct non-local peridynamics theory for thin plates is developed. Peridynamic points are assumed to behave like rigid bodies with independent translation and finite rotation degrees of freedom. The non-local mechanical interaction between points is characterized by force and moment vectors. The balance equations including the linear momentum, the angular momentum and the energy are presented. Peridynamic deformation...
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Konsultacje - Sterowanie Cyfrowe (ACR)
Kursy OnlineMateriały do Sterowania Cyfrowego
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Technika Mikroprocesorowa 2023 - ACR
Kursy OnlineTechnika Mikroprocesorowa dla ACR - materiały: wykład i laboratorium.
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Technika Mikroprocesorowa dla ACR
Kursy OnlineProjektowanie systemów mikroprocesorowych i programowanie w asemblerze.
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Maciej Sperczyński
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A procedure for the determination of dichloromethane and tetrachloroethene in water using pervaporation and gas chromatography
PublikacjaIn the present study, pervaporation was applied to the determination of tetrachloroethene (PCE) and dichloromethane (DCM) in liquid samples. PCE is the most commonly used solvent in dry-cleaning processes. PCE belongs to group 2A of carcinogens (probably carcinogenic to humans) according to the classification of the International Agency for Research on Cancer (IARC). DCM is also widely used as an industrial solvent for the purification...
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Clinical studies of detecting COVID-19 from exhaled breath with electronic nose
PublikacjaThe COVID‑19 pandemic has attracted numerous research studies because of its impact on society and the economy. The pandemic has led to progress in the development of diagnostic methods, utilizing the polymerase chain reaction (PCR) as the gold standard for coronavirus SARS‑CoV‑2 detection. Numerous tests can be used at home within 15 min or so but of with lower accuracy than PCR. There is still a need for point‑of‑care tests available...
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The estimation of total volatile organic compounds emissions generated from peroxide cured natural rubber/polycaprolactone blends
PublikacjaNatural rubber (NR)/polycaprolactone (PCL) blends at the ratio of 90/10% wt. (NR/PCL90/10) and 70/30% wt. (NR/PCL70/30), with a constant amount of dicumyl peroxide, were prepared by compounding in an internal mixer. Obtained NR/PCL bio-based blends were cured at three different temperatures (150 °C, 160 °C and 170 °C). The total content of volatile organic compounds (TVOC) as a function of the NR/PCL blends ratio, and their curing...
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The contingent nature of warehouse flexibility
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From Scores to Predictions in Multi-Label Classification: Neural Thresholding Strategies
PublikacjaIn this paper, we propose a novel approach for obtaining predictions from per-class scores to improve the accuracy of multi-label classification systems. In a multi-label classification task, the expected output is a set of predicted labels per each testing sample. Typically, these predictions are calculated by implicit or explicit thresholding of per-class real-valued scores: classes with scores exceeding a given threshold value...
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Paweł Robert Surowiec mgr lic.
OsobyPhD student at The John Paul II Catholic University of Lublin (Poland), STL degree (specialisation: Ecumenism) awarded in 2022. Roman Catholic priest ordained in 2017. He is interested in the works and theology of saint John Henry Newman (1801-1890). A graduate of catholic Higher Theological Seminary of the Diocese of Sandomierz (Poland) and the John Paul II Catholic University of Lublin. He likes to spend time actively - in prayer,...
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Rapid Multi-band Patch Antenna Yield Estimation Using Polynomial Chaos-Kriging
PublikacjaYield estimation of antenna systems is important to check their robustness with respect to the uncertain sources. Since the Monte Carlo sampling-based real physics simulation model evaluations are computationally intensive, this work proposes the polynomial chaos-Kriging (PC-Kriging) metamodeling technique for fast yield estimation. PC-Kriging integrates the polynomial chaos expansion (PCE) as the trend function of Kriging metamodel...
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Tadeusz Falencikowski dr hab. inż.
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VSC converters control for offshore wind farms HVDC grid connection
PublikacjaThe paper proposes a voltage sourced converter (VSC) new control method. A well-known in the electric power systems correlation between voltage angle and active power and correlation between voltage and reactive power is used instead of feedforward control. This allows for a fast and almost independent control of active and reactive power flow.
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Molecular detection of Candida krusei
PublikacjaThe species identification of fungi belonging to genus Candida is an important issue as this genus becomes the emerging problem of nosocomial infections. As Candida krusei presents intrinsicresistance to the fluconazole that is the drug of the first choice in case of invasive candidiasis the PCR identifying the DNA of C. krusei was elaborated. The analytical sensitivity of the assay on spiked blood samples was estimated at 3-5...
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Isolation and identification of Mycobacterium avium subspecies silvaticum from a horse
PublikacjaPo raz pierwszy opracowano metodę identyfikacji Mycobacterium avium subsp. silvaticum na podstawie analizy sekwencji DNA alfa antygenu. Zastosowanie enzymu HhaI do trawienia produktu PCR z uzyskanego z udziałem starterów specyficznych wobec genu kodującego alfa antygen pozwoliło na identyfikację Mycobacterium avium subsp. silvaticum. Opracowana nowa metoda pozwala na prowadzenie badań epidemiologicznych bezpośrednio z próbek pochodzących...
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ENZO-BIO-PUR Zrównoważone poliuretany: "od kołyski aż po grób" z użyciem enzymów
ProjektyProjekt realizowany w Katedra Technologii Polimerów zgodnie z porozumieniem UMO-2023/05/Y/ST8/00076
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Krzysztof Cwalina dr inż.
OsobyKrzysztof Kamil Cwalina w 2013 r. uzyskał tytuł inżyniera na Wydziale Elektroniki, Telekomunikacji i Informatyki Politechniki Gdańskiej; w 2014 r. uzyskał tytuł magistra inżyniera, a w 2017 r. otrzymał stopień doktora nauk technicznych w dyscyplinie: telekomunikacja, także na WETI PG. Aktualnie pracuje na stanowisku adiunkta w Katedrze Systemów i Sieci Radiokomunikacyjnych Wydziału Elektroniki, Telekomunikacji i Informatyki Politechniki...
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Sensitive determination of isoprostanes in exhaled breath condensate samples with use of liquid chromatography-tandem mass spectrometry
PublikacjaOxidative stress is the hallmark of various inflammatory lung diseases. Increased concentrations of reactive oxygen species in the lungs are reflected by elevated concentrations of oxidative stress markers in the breath, airways, lung tissue and blood. The aim of this work was to develop a method for the fast measurement of F2 isoprostanes in exhaled breath condensate (EBC) samples using equipment which is nowadays available and...
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Relationship between wages, labour productivity and unemployment rate in new EU member countries
PublikacjaThe main aim of this article is to find out the extent to which relative labour productivity and relative unemployment rate changes determine relative wage changes. We use average annual macro-data for the period 2002-2013 for Poland and other 5 new EU members: Estonia, Hungary, Slovak, Czech Republic and Slovenia. Using Poland as benchmark, rst we examine the correlation between wage, productivity and unemployment rate changes...
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Expression of cyclin D1 after treatment with doxorubicin in the HL-60 cell line
PublikacjaIncreased levels of cyclin D1 and amplification of CCND1 gene occur in many types of cancers. We have followed the expression of cyclin D1 after treatment with doxorubicin with reference to cell death and other possible therapeutic implications. The effect of the treatment on the cell cycle, survival, intracellular level (flow cytometry), and intracellular localization of cyclin D1 (fluorescence microscopy) and expression of...
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Evaluation of La 2 Ni 0.5 Cu 0.5 O 4+δ and Pr 2 Ni 0.5 Cu 0.5 O 4+δ Ruddlesden-Popper-type layered oxides as cathode materials for solid oxide fuel cells
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Preliminary studies on the effect of feed speed on the colour change of wood
PublikacjaThis paper presents the results of preliminary analyses of the effect of cutting parameters on changes in the colour of wood. Beech wood cut with use circular saw was analysed. The cutting parameter tested was the feed speed, represented by the feed per tooth. Sawing processes with different feed per tooth ranging from 0.0008 mm to 0.09 mm were analysed. It was observed that over the entire range of feed rate per tooth analysed,...
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Expedited Machine-Learning-Based Global Design Optimization of Antenna Systems Using Response Features and Multi-Fidelity EM Analysis
PublikacjaThe design of antenna systems poses a significant challenge due to stringent per-formance requirements dictated by contemporary applications and the high com-putational costs associated with models, particularly full-wave electromagnetic (EM) analysis. Presently, EM simulation plays a crucial role in all design phases, encompassing topology development, parametric studies, and the final adjustment of antenna dimensions. The latter...
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Possibilities and benefits of a new method of modifying conventional building materials with phase-change materials (PCMs)
PublikacjaThe paper presents a current study devoted to the optimization of the amount of PCM present within traditional building materials, utilized in this case in hollow-brick walls. Authors focus on proposing an alternative to typical direct addition of PCM to existing building materials: introducing two- and three-component mixtures, based on a porous PCM carrier, which in this case was construction aggregate. Preliminary studies of...
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Effects of Post-Harvest Elicitor Treatments with Ultrasound, UV- and Photosynthetic Active Radiation on Polyphenols, Glucosinolates and Antioxidant Activity in a Waste Fraction of White Cabbage (Brassica oleracea var. capitata)
PublikacjaBiosynthesis of phytochemicals in leaves of Brassica can be initiated by abiotic factors. The aim of the study was to investigate elicitor treatments to add value to waste of cabbage. A leaf waste fraction from industrial trimming of head cabbage was exposed to UV radiation (250–400 nm, 59 and 99 kJ m-2, respectively), photosynthetic active radiation (PAR, 400–700 nm, 497 kJ m-2), and ultrasound in water bath (35 kHz, at 15, 30...