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Search results for: HEAT SHOCK PROTEINS
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CHARAKTERYSTYKA WYMIANY CIEPŁA I OPORÓW PRZEPŁYWU OMYWANIA MIKROSTRUGAMI ROZWINIĘTEJ POWIERZCHNI
PublicationW artykule przedstawiono eksperymentalne badania kompaktowego wymiennika ciepła z intensyfikacją przejmowania ciepła przy użyciu mikrostrug. Niniejszy artykuł przedstawia projekt i testy prototypu, wymiennika ciepła typu mikrostrugowego. Modułowa konstrukcja wymiennika umożliwia zmianę wymiarów geometrycznych, a także zmianę materiału przegrody wymiennika ciepła. Metoda graficzna Wilsona została z zastosowana w celu określenia...
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Thermal self-action effects for acoustic beams containing fronts in a Maxwell relaxing fluid
PublicationThis paper examines the thermal self-action of acoustic beams in a Maxwell relaxing fluid. This type of thermal self-action differs from that in a Newtonian fluid and behaves differently depending on a ratio of sound period and time of thermodynamic relaxation. The self-action which relates to sound beams containing shock fronts is also discussed. In addition, stationary and non-stationary types of self-action are considered.
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Calculation of Induced Sheath Voltages in Power Cables – Single Circuit System versus Double Circuit System
PublicationThis paper presents comparison of values of induced sheath voltages in power cable metallic sheaths when one or two cables per phase are used. Calculation of voltages is performed for various phase sequences of the power cables. Three types of the sheaths bonding and earthing are considered. Shock hazard and voltage stress of non-metallic outer sheath of cables are evaluated. The proposed, optimal configuration of the power cable...
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Alanine Scanning Studies of the Antimicrobial Peptide Aurein 1.2
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Antimicrobial Activity of Selected Antimicrobial Peptides Against Planktonic Culture and Biofilm of Acinetobacter baumannii
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Anticandidal Activity of Omiganan and Its Retro Analog Alone and in Combination with Fluconazole
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Probiotic potential of Bacillus Isolates from Polish Bee Pollen and Bee Bread
PublicationThe main goal of this study was the evaluation of the probiotic potential of 10 Bacillus spp. strains isolated from 5 bee bread and 3 bee pollen samples. The antagonistic interaction with Staphylococcus aureus and Escherichia coli was a primary criterion for the preliminary selection of the isolates. Three out of ten strains—PY2.3 (isolated from pollen), BP20.15 and BB10.1 (both isolated from bee bread)—were found to be possible...
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Attenuation of Hyperglycemia in Diabetic Rats Assisted by Immobilized Probiotic in Sodium Alginate
PublicationDiabetes mellitus type 2 (DM2) is the most common chronic disease worldwide, characterized mainly by increased glucose concentration in the blood and affecting several organs’ functionality. The daily consumption of probiotic bacteria can help control diabetes and reduce the damage caused. Cell immobilization techniques are a powerful tool that provides physical cell protection to such probiotic bacteria against gastrointestinal...
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Behavior of residual current devices at frequencies up to 50 kHz
PublicationThe use of residual current devices (RCDs) is obligatory in many types of low-voltage circuits. They are devices that ensure protection against electric shock in the case of indirect contact and may ensure additional protection in the case of direct contact. For the latter purpose of protection, only RCDs of a rated residual operating current not exceeding 30 mA are suitable. Unfortunately, modem current-using equipment supplied...
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Testing sensitivity of A-type residual current devices to earth fault currents with harmonics
PublicationIn many applications, modern current-using equipment utilizes power electronic converters to control the consumed power and to adjust the motor speed. Such equipment is used both in industrial and domestic installations. A characteristic feature of the converters is producing distorted earth fault currents, which contain a wide spectrum of harmonics, including high-order harmonics. Nowadays, protection against electric shock in...
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Computer-aided Analysis of Induced Sheath Voltages in High Voltage Power Cable System
PublicationThis paper concerns the effect of configuration of high voltage power cables on induced voltages in their metallic sheaths. One, two or three cables per phase and phase sequence of the cables are considered. Computing of the voltages was performed for a normal operation and in case of earth fault. Various ways of the sheaths bonding are considered as well. Shock hazard and voltage stress of non-metallic outer sheath of cables are...
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Novel DNA-binding protein from Nanoarchaeum equitans Kin4-M binds all kinds of nucleic acids
PublicationNanoarchaeum equitans is the only known representative of Archaea phylum Nanoarchaeota and stands out as one of the tiniest known living organism. What is more it has smallest genome, which is only 490.885 base pairs long. It is also one of the most compact genomes. According to predictions about 95% of the DNA encodes proteins or stable RNA. Nanoarchaeum equitans lacks genes for most vital metabolic pathways including lipid, cofactor,...
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Thermal Self-Action Effects of Acoustic Beam in a Gas with Reversible or Irreversible Chemical Reaction
PublicationThermal self-action of acoustic beam in a gas where an exothermic chemical reaction takes place, is studied. This kind of thermal self-action differs from that in a newtonian fluid. In dependence on the type of a chemical reaction, reversible or not, heating or cooling of a medium occurs. A beam in the case of the irreversible reaction may be unusually self-focusing. The self-action effects relating to wave beams containing shock...
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ESCASA : Analytical estimation of atomic coordinates from coarse‐grained geometry for nuclear‐magnetic‐resonance ‐assisted protein structure modeling. I. Backbone and Hβ protons
PublicationA method for the estimation of coordinates of atoms in proteins from coarse-grained geometry by simple analytical formulas (ESCASA), for use in nuclear-magnetic-resonance (NMR) data-assisted coarse-grained simulations of proteins is proposed. In this paper, the formulas for the backbone Hα and amide (HN) protons, and the side-chain Hβ protons, given the Cα-trace, have been derived and parameterized, by using the interproton distances...
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Small regulatory bacterial RNAs regulating the envelope stress response
PublicationMost bacteria encode a large repertoire of RNA-based regulatory mechanisms. Recent discoveries have revealed that the expression of many genes is controlled by a plethora of base-pairing noncoding small regulatory RNAs (sRNAs), regulatory RNA-binding proteins and RNA-degrading enzymes. Some of these RNA-based regulated processes respond to stress conditions and are involved in the maintenance of cellular homeostasis. They achieve...
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Investigation of interaction between xanthene dye rhodamine B and metagenomic-derivated methylthioadenosine phosphorylase RSFP
PublicationIn the previous study we found that the expression of the metagenomic - derived methylthioadenosine phosphorylase gene (rsfp) in E. coli cells and the presence of rhodamine B in the growth medium were crucial for the pink fluorescence of one E. coli colony from Antarctic soil metagenomic library (Cieśliński et al., 2009). In this study, rsfp gene expression was induced by an arabinose promoter in an LMG194 E. coli strain and the...
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Molecular basis and quantitative assessment of TRF1 and TRF2 protein interactions with TIN2 and Apollo peptides
PublicationShelterin is a six-protein complex (TRF1, TRF2, POT1, RAP1, TIN2, and TPP1) that also functions in smaller subsets in regulation and protection of human telomeres. Two closely related proteins, TRF1 and TRF2, make high-affinity contact directly with double-stranded telomeric DNA and serve as a molecular platform. Protein TIN2 binds to TRF1 and TRF2 dimer-forming domains, whereas Apollo makes interaction only with TRF2. To elucidate...
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Nuclear Co-generation: The Analysis of Technical Capabilities and Cost Estimates
PublicationThis paper presents a concept of the parallel connection of a nuclear power plant fitted to provide heat for district heating application, with the CHP and heat plants existing in the supply region, in this case with the heating systems of Wejherowo and Gdynia. Presented variant proposes to add heat to a nuclear power plant’s total output by supplying heat exchangers with the steam from bleeders of low pressure (LP) turbine stage...
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Analytical and reliability data from the real-time simulator biogas plant
Open Research DataThe dataset represents the results of biogas plant simulation. The described plant is an agricultural biogas system, which can produce electrical energy with power estimated up to 1070 kW and heat energy that can reach 1200 kW simultaneously.
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Cavity-expansion approximation for projectile impact and penetration into sand
PublicationA one-dimensional problem of a spherical cavity expanding at a constant velocity from zero initial radius in an infinite granular medium, which has the first-kind self-similar solution, is considered. We are solving this dynamic spherical cavity-expansion problem to model rigid spheres penetrating into a granular media. Elastic–plastic deformation of the granular media is described in a barotropic approximation, using the high-pressure...
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Properties of water in the region between a tubulin dimer and a single motor head of kinesin
PublicationA kinesin is a molecular motor that can perform movement on a microtubule track in a stepping-like manner. This motion is connected with processes of association and dissociation of kinesin and tubulin. Water is an important participant in these kinds of molecular interactions. This is why we have decided to investigate the dynamical and structural properties of water in the region between the kinesin catalytic domain and the tubulin...
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Telomere uncapping by common oxidative guanine lesions: Insights from atomistic models
PublicationOxidative damage to DNA is widely known to contribute to aging and disease. This relationship has been extensively studied for telomeres – structures that cap chromosome ends – due to their role in cell proliferation and senescence, and exceptional susceptibility to oxidation. Indeed, the repetitive telomeric DNA sequence contains the 5′-GGG-3′ motif that has the lowest ionization potential of all trinucleotides. Accordingly, experiments...
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RESEARCH OF HYDRAULIC PISTON PUMPS’ START-UP IN LOW AMBIENT TEMPERATURES
PublicationStarting hydraulic pumps in low ambient temperatures may cause: decreased hydraulic-mechanical efficiency, larger vibration of hydraulic installation, faster wear and more frequent damage to the elements of pumps, change of material properties of elements, increase of pressure drop. As a result of increase of resistance of cold oil flow in the suction pipe, large underpressure may be experienced, causing incomplete filling of pump...
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In Silico Safety Assessment of Bacillus Isolated from Polish Bee Pollen and Bee Bread as Novel Probiotic Candidates
PublicationBacillus species isolated from Polish bee pollen (BP) and bee bread (BB) were characterized for in silico probiotic and safety attributes. A probiogenomics approach was used, and in-depth genomic analysis was performed using a wide array of bioinformatics tools to investigate the presence of virulence and antibiotic resistance properties, mobile genetic elements, and secondary metabolites. Functional annotation and Carbohydrate-Active...
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Free convection of water-Al2O3 nanofluid from horizontal porous coated tube
PublicationNanofluids are expected to enhance heat transfer in many thermal systems. Present efforts are concentrated on boiling heat transfer of nanofluids, although single phase convection is of key importance in various appliances such as car radiators or heating systems. This paper presents experimental results of heat transfer during free convection of water-Al3O3 nanofluid from horizontal tube covered with metallic porous coating. Contrary...
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Reactions on the surface of the implant under the influence of biofilm
PublicationThe contact of a biomaterial with the biological environment in in vitro and in vivo tests leads to the production of a particular ecosystem in which the active roles perform both, the material surface and the extracellular matrix protein forming a biofilm. Proteins affect cell and bacteria adhesion processes, biological activity of cells and activation of inflammatory response.The knowledge of the reaction mechanisms active on...
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EPR of TiO2 hydrogenated at 500 and 600oC
Open Research DataData contain EPR spectra of TiO2 (anatase phase) heat treated in H2 at 500 and 600oC. Sample heated at 500oC contained 4 wt% of rutile and that at 600oC 8 wt% of rutile.
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NEW TRENDS IN DEVELOPMENT OF ORC'S FOR MICROPOWER GENERATION
PublicationIn the paper, new trends in development of micropower generation of heat and electricity are presented. New type of CHP for domestic usage is developed in the Institute of Fluid-Flow Machinery PAS and methods of its design are presented. The most promising trends in equipment of ORC cycle for this purpose were discussed. Main attention was focused on micro-heat exchangers design based on micro-channels and micro-jets. In our opinion...
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Fuzyjne polimerazy DNA – otrzymywanie, charakterystyka i zastosowanie
PublicationObecnie reakcje PCR (ang. Polymerase Chain Reaction) wykazują bardzo szerokie zastosowanie w diagnostyce medycznej, biologii molekularnej czy inżynierii genetycznej. Efektywność tych reakcji rozumiana jako wydajność i wierność przeprowadzonej amplifikacji jest nieodłącznie związana ze stosowaną polimerazą DNA i warunkami prowadzenia reakcji PCR. Aby sprostać wymaganiom stawianym przez nowoczesne metody diagnostyczne oraz współczesną...
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XPS-TiO2 heated in H2
Open Research DataThese XPS spectra were recorded for TiO2 heat-treated at 450-600oC in H2. Surface oxygen groups were determined. Deconvolution of Ti 2p peak allowed to determine Ti3+ on the titania surface.
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SEM of TiO2 heated at 400-600oC in Ar or H2
Open Research DataData contain some SEM images of TiO2 taken after its heat treatment in Ar or H2 at 400-600oC. Treated TiO2 was crystalline anatase phase with 3-4 % of rutile nclei.
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Unusual structural properties of water within the hydration shell of hyperactive antifreeze protein
PublicationMany hypotheses can be encountered explaining the mechanism of action of antifreeze proteins. One widespread theory postulates that the similarity of structural properties of solvation water of antifreeze proteins to ice is crucial to the antifreeze activity of these agents. In order to investigate this problem, the structural properties of solvation water of the hyperactive antifreeze protein from Choristoneura fumiferana were...
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Proteolysis of whey protein isolates in nanoemulsion systems: impact of nanoemulsification and additional synthetic emulsifiers
PublicationNanoemulsions are currently of interest in the functional food sector because their small droplet size (100–500 nm) provides a number of potential advantages over conventional emulsions. This study concerned the behavior of nanoemulsions stabilized with whey proteins and two synthetic emulsifiers (Tween 80 and Croduret), and exposed to conditions simulating the human upper gastrointestinal tract. In particular, the effect of synthetic...
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Fibrillar aggregates in powdered milk
PublicationThis research paper addresses the hypothesis that powdered milk may contain amyloid fibrils. Amyloids are fibrillar aggregates of proteins. Up to this time, research on the presence of amyloids in food products are scarce. To check the hypothesis we performed thioflavin T fluorescence assay, X-ray powder diffraction, atomic force microscopy and fluorescence microscopy imaging. Our preliminary results show that commercially available...
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Effect of Pin Shape on Thermal History of Aluminum-Steel Friction Stir Welded Joint: Computational Fluid Dynamic Modeling and Validation
PublicationThis article studied the effects of pin angle on heat generation and temperature distribution during friction stir welding (FSW) of AA1100 aluminum alloy and St-14 low carbon steel. A validated computational fluid dynamics (CFD) model was implemented to simulate the FSW process. Scanning electron microscopy (SEM) was employed in order to investigate internal materials’ flow. Simulation results revealed that the mechanical work...
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A numerical analysis of the thermal effects in the jet impingement stagnation zone
PublicationMost of the flows occurring in the engineering systems are turbulent and their accurate numerical analysis is still challenging, especially when combined with the heat transfer. One of the methods of heat transfer enhancement is utilization of the turbulent impinging jets, which were recently applied also in the heat exchangers. Their positive impact on the heat transfer performance was proven, but many questions related to its...
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Phase change thermal energy storage – the experience of the materials preparation for the specific applications.
PublicationThermal energy storage and temperature stabilization is very important in many engineering applications. There are three kinds of thermal energy storage: sensible heat, latent heat and reversible chemical reaction heat. Phase change materials (PCM) absorb, store and release large amounts of energy in the form of latent heat, at constant temperature, called the transition temperature. Many innovative applications could be found...
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A new factor LapD is required for the regulation of LpxC amounts and lipopolysaccharide trafficking
PublicationLipopolysaccharide (LPS) constitutes the major component of the outer membrane and is essential for bacteria, such as Escherichia coli. Recent work has revealed the essential roles of LapB and LapC proteins in regulating LPS amounts; although, if any additional partners are involved is unknown. Examination of proteins co-purifying with LapB identified LapD as a new partner. The purification of LapD reveals that it forms a complex...
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Novel primosomal protein B from Clostridium pastuerianum
PublicationPriB is a primosomal protein that catalyzes DNA replication in Procaryota. The replication pathway starts with PriA protein - the initiator protein that binds to a DNA replication fork, unwinds double-stranded DNA and role of PriB is to stabilize PriA on the DNA. However there are many biochemical differences in replication mechanism in bacteria and only some of them use PriB proteins. A few of PriB proteins were published and...
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Novel primosomal protein B from Clostridium pasteurianum
PublicationPriB is a primosomal protein that catalyzes DNA replication in Procaryota. The replication pathway starts with PriA protein - the initiator protein that binds to a DNA replication fork, unwinds double-stranded DNA and role of PriB is to stabilize PriA on the DNA. However there are many biochemical differences in replication mechanism in bacteria and only some of them use PriB proteins. A few of PriB proteins were published and...
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Novel anticancer strategy aimed at targeting shelterin complexes by the induction of structural changes in telomeric DNA: hitting two birds with one stone.
PublicationThe ends of chromosomes in mammals are composed of telomeric DNA containing TTAGGG repeats, which bind specific proteins called shelterins. This telomeric DNA together with shelterins form a cap that protects the ends of chromosomes from being recognized as sites of DNA damage and from chromosomal fusions. Many very successful antitumor drugs used in the treatment of cancer patients bind to DNA, some of them with a prominent sequence...
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Modification of the operating point of residual current transformers for high frequency earth fault currents detection
PublicationFor protection against electric shock in low voltage systems residual current devices are commonly used. However, their proper operation can be interfered when earth fault current with high frequency components occurs. Serious hazard of electrocution exists then. One of the most important element of residual current devices is a residual current transformer with iron core. Tripping characteristic of residual current devices strictly...
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Przegląd rodzajów chiralnych faz stacjonarnych oraz możliwości ich zastosowań w chromatografii cieczowej
PublicationChromatograficzne rozdzielanie związków optycznie czynnych ma ogromne znaczenie nie tylko w przemyśle farmaceutycznym, ale i agrochemicznym, a także w badaniach naukowych różnego rodzaju. W niniejszym opracowaniu scharakteryzowano komercyjnie dostępne chiralne fazy stacjonarne na bazie, cyklodekstryn, polisacharydów, makrocyklicznych antybiotyków, eterów koronowych, a także fazy proteinowe, ligandowymienne, jonowymienne oraz fazy...
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Adapting the pinch point analysis to improve the ORC design process
PublicationPinch point analysis can be adapted so as to assist in the early stages of designing the ORC systems. Typically used in determining the working parameters of heat exchangers, it can be employed to improve the heat fitting between the source of heat and the working fluids of the ORC system. To attain the parametric match, an algorithm was built enabling quick estimation of the possible heat reception by the specific working fluid....
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MIC1-MAG1-SAG1 chimeric protein, a most effective antigen for detection of human toxoplasmosis
PublicationThis study describes a Toxoplasma gondii IgG ELISA test based on a new chimeric antigen containing three immunodominant regions from MIC1, MAG1 and SAG1 proteins of a parasite and shows that this test is useful for diagnostic purposes and may replace the lysed and whole-cell antigens.
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FTIR-TiO2 heated at 400-600oC in Ar or H2
Open Research DataData contain FTIR diffuse eflectance spectra of TiO2, which was heat-treated at 400-600oC in Ar or H2. Hydrogen reduced TiO2 revealed new bands due to the adsorption of some species on the titania oxygen vacancy sites.
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Profitability criteria of partial cogeneration in nuclear power plant.
PublicationIn this paper, profitability criteria and calculation methodology of profitability conditions for partial cogeneration in nuclear power plant (NPP) was presented. Considered NPP supplies electricity to consumers through public power system, whereas heat is expected to be supplied to consumption regions via long-distance heat transport line. First proposed profitability criterion was total annual costs of electricity and heat delivered...
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Improved energy management technique in pipe-embedded wall heating/cooling system in residential buildings
PublicationEffective and environmentally responsive techniques of energy management in residential buildings are desirable for the resulting reduction of energy costs and consumption. In this paper, an improved and efficient technique of energy management in pipe-embedded wall heating/cooling systems, called the Thermal Barrier, is described. Specifically, the Thermal Barrier is a technique focused on the management and control of heat...
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Improved energy management technique in pipe-embedded wall heating/cooling system in residential buildings
PublicationEffective and environmentally responsive techniques of energy management in residential buildings are desirable for the resulting reduction of energy costs and consumption. In this paper, an improved and efficient technique of energy management in pipe-embedded wall heating/cooling systems, called the Thermal Barrier, is described. Specifically, the Thermal Barrier is a technique focused on the management and control of heat supply...
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Multicriteria Optimization Approach to Design and Operation of District Heating Supply System over its Life Cycle
PublicationDistrict Heating (DH) systems are commonly supplied using local heat sources. Nowadays, modern insulation materials allow for effective and economically viable heat transportation over long distances (over 20 km). In the paper a method for optimized selection of design and operating parameters of long distance Heat Transportation System (HTS) is proposed. The method allows for evaluation of feasibility and effectivity of heat transportation...