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Search results for: HUMAN DETECTION
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Histogram of Gradients with Cell Average Intensity for Human Detection
PublicationThe modification of the descriptor in human detector using Histogram of Oriented Gradients and support vector machine is presented. The proposed modification requires inserting the average cell intensitiesresulting with the increase of the length of the descriptor from 3780 to 4200 values, but it is easy to compute and instantly gives 14-26% of miss rate improvement at 10^-4 False Positives Per Window (FPPW). The modification...
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Histogram of Oriented Gradients with Cell Average Brightness for Human Detection
PublicationA modification of the descriptor in a human detector using Histogram of Oriented Gradients (HOG) and Support Vector Machine (SVM) is presented. The proposed modification requires inserting the values of average cell brightness resulting in the increase of the descriptor length from 3780 to 3908 values, but it is easy to compute and instantly gives ≈ 25% improvement of the miss rate at 10‒4 False Positives Per Window (FPPW). The...
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A novel chemiluminescent immunoassay for detection of Toxoplasma gondii IgG in human sera
PublicationThis study describes Toxoplasma gondii IgG chemiluminescent immunoassay (CLIA) based on the use of a novel immunochemical reagents in the form of the conjugates of original acridinium ester (AE) labels attached to antibodies and SAG2-GRA1-ROP1L chimeric antigen and shows that this test is useful for diagnostic purposes.
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MIC1-MAG1-SAG1 chimeric protein, a most effective antigen for detection of human toxoplasmosis
PublicationThis study describes a Toxoplasma gondii IgG ELISA test based on a new chimeric antigen containing three immunodominant regions from MIC1, MAG1 and SAG1 proteins of a parasite and shows that this test is useful for diagnostic purposes and may replace the lysed and whole-cell antigens.
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Artificial intelligence support for disease detection in wireless capsule endoscopy images of human large bowel
PublicationIn the work the chosen algorithms of disease recognition in endoscopy images were described and compared for theirs efficiency. The algorithms were estimated with regard to utility for application in computer system's support for digestive system's diagnostics. Estimations were achieved in an advanced testing environment, which was built with use of the large collection of endoscopy movies received from Medical University in Gdańsk....
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Determination of tocopherols and tocotrienols in human breast adipose tissue with the use of high performance liquid chromatography-fluorescence detection
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Development of the first internally-quenched fluorescent substrates of human cathepsin C: The application in the enzyme detection in biological samples
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Development of an Analytical Protocol for Determination of Cyanide in Human Biological Samples Based on Application of Ion Chromatography with Pulsed Amperometric Detection
PublicationA simple and accurate ion chromatography (IC) method with pulsed amperometric detection (PAD) was proposed for the determination of cyanide ion in urine, sweat, and saliva samples. The sample pretreatment relies on alkaline digestion and application of Dionex OnGuard II H cartridge. Under the optimized conditions, the method showed good linearity in the range of 1–100 μg/L for urine, 5–100 μg/L for saliva, and 3–100 μg/L for sweat...
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Multisine impedimetric probing of biocatalytic reactions for label-free detection of DEFB1 gene: How to verify that your dog is not human?
PublicationAlbert is a dog (Canis familiaris), but he does not realize this. Albert loves human food (and beer), watching movies on the internet, sleeping in bed, and more. But he should not do all these things. To convince him that, we have desinged a test procedure. The DEFB1 gene is unique to human species. Detecting its presence from saliva and in short periods may offer an advantage in the field of forensic medicine, and influence Albert’s...
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Multimodal detection and analysis of a new type of advanced Heinz body-like aggregate (AHBA) and cytoskeleton deformation in human RBCs
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Human red blood cells targeted metabolome analysis of glycolysis cycle metabolites by capillary electrophoresis using an indirect photometric detection method
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Highly Specific Substrates of Proteinase 3 Containing 3-(2-Benzoxazol-5-yl)-l-alanine and Their Application for Detection of This Enzyme in Human Serum
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The first study on the usefulness of recombinant tetravalent chimeric proteins containing fragments of SAG2, GRA1, ROP1 and AMA1 antigens in the detection of specific anti-Toxoplasma gondii antibodies in mouse and human sera
PublicationThis study presents an evaluation of four tetravalent recombinant chimeric proteins containing fragments of the Toxoplasma gondii antigens, SAG2, GRA1, ROP1 and AMA1, as potential replacements of a the soluble, whole-cell tachyzoite lysate (TLA) used in serological assays. Recombinant chimeric proteins (SAG2-GRA1-ROP1-AMA1N, AMA1N-SAG2-GRA1-ROP1, AMA1C-SAG2-GRA1-ROP1, and AMA1-SAG2-GRA1-ROP1) obtained by genetic engineering were...
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An electrochemical biosensor for the determination of hormone Human Chorionic Gonadotropin (hCG) in human serum
PublicationThis work describes the modification of a gold electrode to create an electrochemical biosensor capable of detecting human chorionic gonadotropin (hCG). The biosensor was obtained by modifying the gold electrode with cysteamine and oligopeptide (PPLRINRHILTR). The modification steps of the gold electrode were confirmed by cyclic voltammetry (CV) and impedance electrochemical spectroscopy (EIS) measurements. The conducted EIS experiments...
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New sample preparation strategies for comprehensive lipidomics of human breast milk
PublicationGLOBAL PROFILING ANALYSIS OF HUMAN BREAST MILK (HBM) LIPIDS IS HINDERED BY COMPLEX COMPOSITION OF HBM. THIS PROBLEM IS HUGE FROM ANALYTICAL POINT OF VIEW, SINCE DUE TO THAT DETECTION OF ALL HBM LIPIDS DURING ONE ANALYTICAL RUN IS LIMITED. THUS, SAMPLE PREPARATION STEP CONSTITUTES A CRUCIAL STEP IN UNTARGETED LIPIDOMIC ANALYSIS OF HBM, ESPECIALLY WHEN SEMI-QUANTITATIVE ANALYSIS IS ASSUMED. HEREIN, WE PRESENT A COMPARISON OF PUBLISHED...
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Human-Computer Interface Based on Visual Lip Movement and Gesture Recognition
PublicationThe multimodal human-computer interface (HCI) called LipMouse is presented, allowing a user to work on a computer using movements and gestures made with his/her mouth only. Algorithms for lip movement tracking and lip gesture recognition are presented in details. User face images are captured with a standard webcam. Face detection is based on a cascade of boosted classifiers using Haar-like features. A mouth region is located in...
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Sensing Direction of Human Motion Using Single-Input-Single-Output (SISO) Channel Model and Neural Networks
PublicationObject detection Through-the-Walls enables localization and identification of hidden objects behind the walls. While numerous studies have exploited Channel State Information of Multiple Input Multiple Output (MIMO) WiFi and radar devices in association with Artificial Intelligence based algorithms (AI) to detect and localize objects behind walls, this study proposes a novel non-invasive Through-the-Walls human motion direction...
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Response of a New Low-Coherence Fabry-Perot Sensor to Hematocrit Levels in Human Blood
PublicationIn this paper, a low-coherence Fabry-Perot sensor with a spectrally measured signal processing response to the refractive index of liquids is presented. Optical fiber sensors are potentially capable of continuous measuring hematocrit levels in blood. Low-coherence Fabry-Perot interferometric sensors offer a robust solution, where information about the measurand is encoded in the full spectrum of light reflected from the sensing...
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PCR detection of Scopulariopsis brevicaulis
PublicationScopulariopsis brevicaulis is known as a most common etiological factor of the mould toenail infections. There are also reports indicating that S. brevicaulis could cause organ and disseminated infections. Nowadays microscopic observations from the direct sample and culture are crucial for the appropriate recognition of the infection. In this paper is presented a PCR-based method for S. brevicaulis detection. The specificity of...
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A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test
PublicationToxoplasma gondii infection is one of the most common human zoonosis. Laboratory diagnosis of this disease is mainly based on the results of serological methods detecting specific antibodies in the patient’s sera. In this study we aimed to evaluate the performance of a chemiluminescence immunoassay (CLIA) based on the use of a novel immunochemical reagent in the form of the conjugate of original acridinium label (AL) attached to...