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Search results for: PROTEIN FOLDING
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Taurine as a water structure breaker and protein stabilizer
PublicationThe enhancing effect on the water structure has been confirmed for most of the osmolytes exhibiting both stabilizing and destabilizing properties in regard to proteins. The presented work concerns osmolytes, which should be classified as “structure breaking” solutes: taurine and N,N,N-trimethyltaurine (TMT). Here, we combine FTIR spectroscopy, DSC calorimetry and DFT calculations to gain an insight into the interactions between...
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Protein thermal stabilization in aqueous solutions of osmolytes
PublicationProteins’ thermal stabilization is a significant problem in various biomedical, biotechnological, and technological applications. We investigated thermal stability of hen egg white lysozyme in aqueous solutions of the following stabilizing osmolytes: Glycine (GLY), N-methylglycine (NMG), N,N-dimethylglycine (DMG), N,N,N-trimethylglycine (TMG), and trimethyl-N-oxide (TMAO). Results of CD-UV spectroscopic investigation were compared...
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ANALYSIS OF THE p53 PROTEIN GENE EXPRESSION MODEL
PublicationWe study the asymptotic behaviour of the solutions of the p53-Mdm2 model proposed by Monk (2003). The p53 gene is crucial for cellular inhibition of the angiogenesis process, while Mdm2 is a negative regulator of the p53 tumor-suppressor. We investigate the stability of the positive steady state and perform some numerical experiments.
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Strouhal Number Measurement for Novel Biomimetic Folding Fins Using an Image Processing Method
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Statistical Dynamics of the Rolling Mills
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Falling accident risk in Poland.
PublicationFor the last few years we observe optimistic tendencies in traffic risk development in Poland the number of fatalities decreases about 10% per year, bringing Poland closer to European Union standards. Nevertheless in 2001 we observed the unexpected, and bigger than forecasted, casualty reduction. The reason was the economic downturn suffered across the country, resulting in reduced demand for transportation. The danger lies in...
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Falling accident risk in Poland.
PublicationFor the last few years we observe optimistic tendencies in traffic risk development in Poland - the number of fatalities decreases about 10% per year, bringing Poland closer to European Union standards. Nevertheless in 2001 we observed the unexpected, and bigger than forecasted, fatality reduction. The reason was the economic downturn suffered across the country, resulting in reduced demand for transportation. The danger lies in...
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Location of ship rolling axis.
PublicationPodano definicję osi kołysań statku i metodę określania jej położenia. Położenie osi zależy nie tylko od rozkładu mas statku ale równiez od tzw. ''mas towarzyszących'', które są reprezentacją sił hydrodynamicznych działających na statek w czasie kołysania. Podano proste formuły pozwalajace obliczyć poprawne położenie osi kołysań statku.
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Characterization of a single-stranded DNA-binding-like Protein from Nanoarchaeum equitans - a nucleic acid binding protein with broad substrate specificity
PublicationBackground SSB (single-stranded DNA-binding) proteins play an essential role in all living cells and viruses, as they are involved in processes connected with ssDNA metabolism. There has recently been an increasing interest in SSBs, since they can be applied in molecular biology techniques and analytical methods. Nanoarchaeum equitans, the only known representative of Archaea phylum Nanoarchaeota, is a hyperthermophilic, nanosized,...
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Mechanical Recycling via Regrinding, Rebonding, Adhesive Pressing, and Molding
PublicationIncreasing amount of polyurethane foams waste (e.g. from the building or furniture industry) produced every year resulted in the intensive development of their recycling methods. This chapter covers most important mechanical recycling methods, i.e. regrinding, rebonding, adhesive pressing, and molding. The procedure, required equipment and chemicals (if needed) for each method were described. The possible applications of the products...
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Novel primosomal protein B from Clostridium pastuerianum
PublicationPriB is a primosomal protein that catalyzes DNA replication in Procaryota. The replication pathway starts with PriA protein - the initiator protein that binds to a DNA replication fork, unwinds double-stranded DNA and role of PriB is to stabilize PriA on the DNA. However there are many biochemical differences in replication mechanism in bacteria and only some of them use PriB proteins. A few of PriB proteins were published and...
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Lipid-protein interactions in membranes: a molecular modelling study
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Effect of increased protein intake on the risk of female infertility
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A bifunctional chimeric protein consisting of MutS and beta-galactoside
PublicationPraca pokazuje konstrukcję DNA plazmidowego kodującego bifunkcjonalne białko chimeryczne złożone z MutS Thermus thermophilus i beta-galaktozydazy E.coli, optymalizację jego ekspresji i oczyszczania. Białko to testowano w metodzie wykrywania mutacji punktowych, wykorzystując kolorymetryczne mierzenie aktywności domeny reporterowej beta galaktozydazy.
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Construction and purification of his6-Thermus thermophilus MutS protein
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A bifunctional chimeric protein consisting of MutS and beta-galactosidase
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The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana
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The SERRATE protein is involved in alternative splicing in Arabidopsis thaliana
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Role of Oligosaccharide Chain Polarity in Protein–Glycosaminoglycan Interactions
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Copper containing protein sites - structure, properties and models
PublicationEnzymy organizmów żywych katalizują wiele reakcji stosowanych w przemyśle chemicznym. Przykładem potencjalnego zastosowania enzymu w produkcji jest synteza L-Dopy przy udziale tyrozynazy. Ze względu na możliwe praktyczne zastosowanie w katalizie, otrzymano i opisano wiele prostych modeli miejsc aktywnych enzymów. W artykule opisano niektóre modele białek zawierających miedź.
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Electrosynthesis of potato starch–whey protein isolate complexes
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Construction and purification of his6-Thermus thermophilus MutS protein.
PublicationGen mutS z termofilnej bakterii Thermus thermophilus zamplifikowano przy użyciu PCR, sklonowano i przeprowadzono jego ekspresję w E. coli. Rekombinantowe białko MutS zawierające oligohistydynową domenę na N-końcu oczyszczono w jednoetapowej procedurze przy wykorzystaniu chromatografii powinowactwaNi(2+). Zdolność do rozpoznania niekomplementarności białka his(6)-MutS potwierdzono w eksperymentach ochrony DNA przed trawieniem...
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Novel primosomal protein B from Clostridium pasteurianum
PublicationPriB is a primosomal protein that catalyzes DNA replication in Procaryota. The replication pathway starts with PriA protein - the initiator protein that binds to a DNA replication fork, unwinds double-stranded DNA and role of PriB is to stabilize PriA on the DNA. However there are many biochemical differences in replication mechanism in bacteria and only some of them use PriB proteins. A few of PriB proteins were published and...
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Conserved motifs of MutL proteins
PublicationtThe MutL protein is best known for its function in DNA mismatch repair (MMR). However, there isevidence to suggest that MutL is not only the linker connecting the functions of MutS and MutH in MMR,but that it also participates in other repair systems, such as Very Short Patch (VSP), Base Excision (BER)and Nucleotide Excision Repair (NER). This study set out to identify the most highly conserved aminoacid sequence motifs in MutL...
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Concentration of proteins in the whey stream
PublicationZastosowano metodę separacji pianowej do odzyskiwania białka z serwatki. Wykazano silny wpływ prędkości powietrza w kolumnie, początkowego stężenia białka w serwatce i pH roztworu na przebieg i skuteczność flotacji. Współczynnik wzbogacenia maleje ze wzrostem prędkości przepływu powietrza oraz ze wzrostem początkowego stężenia białka serwatkowego. Najwyższą wartość współczynnika wzbogacenia uzyskano dla pH naturalnej serwatki.
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Conservation and diversity of MutS proteins
PublicationThe homologues of MutS, mismatch repair protein, exist in all prokaryotes, with the exception of Actinobacteria, Mollicutes and part of the Archaea. Multiple alignments of 316 MutS amino acid sequences from 169 species revealed conserved residues and sequence motifs distinguishing MutS homologues. All MutS homologues show high conservation within the ATPase domain. MutS1, the homologue responsible for DNA mismatch recognition,...
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Numerical modelling of different airbag folding patterns and their influence on occupant responses in frontal vehicle impact
PublicationThis paper presented a procedure for airbag folding for the application in occupant safety studies and analysed the influence of different airbag folding patterns on the occupant severity in frontal impact. Airbags were folded in two patterns: zig-zag and top-roll, using two folding techniques: Initial Metric Method and Explicit Folding. The explicit folding was found to be more expensive in terms of preparation time. However,...
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Monocyte Chemoattractant Protein-Induced Protein 1 (MCPIP1) Enhances Angiogenic and Cardiomyogenic Potential of Murine Bone Marrow-Derived Mesenchymal Stem Cells
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Protein overproduction alters exosome secretion in Chinese hamster ovary cells
PublicationDespite the abundance of available cell lines, nearly 70% of all recombinant therapeutic proteins today are produced in Chi-nese hamster ovary (CHO) cells. The impact of protein overproduction on the secretion of exosomes by CHO cells has been investigated here. Increased secretion of extracellular vesicles (EVs) by protein overexpressing CHO cells was demonstrated with protein content assay, nanoparticle tracking analysis, and...
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Role of bead sequence in underwater welding
PublicationThis paper presents examinations of the role of the bead sequence in underwater welding. Two specimens of wet welded layers made by covered electrodes with the use of normalized S355G10+N steel were welded by a reasonable bead sequence. For each specimen, metallographic macro- and micro-scopic tests were done. Then, Vickers HV10 hardness measurements were conducted for each pad weld in the welded layer. The results show that welding...
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Type of surface and tire rolling resistance
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Rolling resistance measurements of studded tires
PublicationW pracy przedstawiono wyniki pomiaru oporu toczenia opon kolcowanych i po usunięciu kolców na nawierzchni poroelastycznej. Badania przeprowadzono na stanowisku bębnowym Katedry Pojazdów i Maszyn Roboczych Politechniki Gdańskiej. Opony kolcowane mają o około 5% wyższy współczynnik oporu toczenia w porównaniu z oponami nie kolcowanymi.
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Underwater Welding of Duplex Stainless Steel
PublicationThe present work was conducted to assess the weldability of duplex stainless steel at underwater conditions. Interest of underwater welding of this steel grade is connected with necessity of preparing welding repair technologies for subsea pipelines widely used in offshore oil and gas industry. The GMA local cavity welding method was used in the investigations. Welded beads were performed underwater at 0.5 m depth and in the air....
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Review of titanium and steel welding methods
PublicationThe paper presents review of methods of titanium and steel welding. On basis of professional literature it was stated that titanium can be joined with steel by means of explosive welding or brazing.Joining titanium with steel by TIG welding and friction welding methods is possible with the use of copper and tantalum or vanadium interlayer.
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Modelling of pipes conveying flowing liquid
PublicationThe paper introduces the method of modal reduction of system which consists of pipe with flowing liquid. The concept of hybrid model is proposed. The system model consists of two parts, the modal model and the finite elements model. The modal model represents linear, self-adjoined part of the system, while simple lumped technique is applied for modelling of Coriolis phenomena.
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An efficient algorithm for finding ideal schedules
PublicationPodejmujemy problem szeregowania zadań jednostkowych z zadanymi czasamy przybycia i zależnościami kolejnościowymi. Uszeregowanie jest idealne jeśli jednocześnie minimalizuje maksymalny oraz średni czas zakończenia zadania. Podajemy przyklad pokazujący, że uszeregowania idealne nie istnieją dla relacji zależności zadań będącej drzewem, gdy dopuścimy możliwość wystąpienia przerwań. Z drugiej strony podajemy algorytm o złożoności...
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Filling of carbon nanotubes for bio‐applications
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Technology of welding hard wearing plates
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Unfolding new values in the landscape of conflict
PublicationThe main objective of the paper is to investigate how the conflict quality of landscape can be used as its asset and potential for creating new values. Like mediation between different overlapping archaeologies offers a basis for the perception of place in the chronology of time, the mediation between conflicted areas creates a capacious zone for enhancing the unique quality of landscape in its architectural, social, cultural and...
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Theoretical and practical solving of hydroengineering problems
PublicationRozważono wzajemne relacje pomiędzy znaczeniem terminów ''teoretyczny'' i ''praktyczny'', w kontekście problematyki nauk technicznych, analizujac aspekty terminologiczne, poznawcze, wolicjonalne i formalne. Wskazano na potrzebę różnicowania ścisłych i potocznych znaczeń uzywanych terminów.
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Forming and Ranking Musical Rhythm Hypotheses.
PublicationW pracy przedstawiono podstawowe pojęcia i definicje zwiazne z wyszukiwaniem informacji rytmicznej w utworach muzycznych. W muzykologii przyjmuje się, że atrybuty dźwięku, takie jak długość, częstotliwość oraz amplituda dźwięku determinują wagę rytmiczną dźwięku. W artykule przebadano te właściwości fizyczne dźwięku w kontekście okreslenia wagi rytmicznej, czyli miary określającej tendencję dźwięku do znalezienia się na początku...
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Extremal thermal loading of a bifurcation pipe
PublicationThe subject of considerations is a spherical bifurcation pipe of a live steam made of steel P91, which is an element of a block of coal-fired power plant working with a 18K370 turbine. As experience shows, it is a very sensitive element of the boiler pipelines. An extreme work scenario for such a block has been adopted, in which the turbine is shutting down to a warm state three times in 24 hours. This is an action dictated by...
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Final report on noise and rolling resistance
PublicationWork Package 6 deals with environmental impacts of PERS use, including noise and rolling resistance. This report covers preliminary noise tests performed in the laboratory and at the road test sites. The obtained results indicate that PERS material reduce noise considerably - up to 12 dB in comparison to SMA16 reference surface. Noise reduction properties are especially visible in the case of factory produced PERS slabs supplied...
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Methods of Tire Rolling Resistance Measurements
PublicationTire rolling resistance is one of the factors related to tire/road interaction that has important influence on road vehicles performance. Raising fuel price and increased concern related to the environmental changes result in growing interest in reduction of tire rolling resistance that influences fuel consumption, especially in free-flowing highway traffic with moderate speeds. There are several methods of tire rolling resistance measurements....
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INFLUENCE OF TEMPERATURE ON THE TYRE ROLLING RESISTANCE
PublicationTemperature is a very important factor controlling rolling resistance of road vehicle tyres. There are at least three different temperatures that may be considered as important factors controlling thermal conditions of the rolling tyre. The most common measure of the thermal conditions during tyre rolling is ambient air temperature. The other two are: pavement temperature and “tyre” temperature. Tyre temperature is the most difficult...
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Forming of explosively welded metal plates.
PublicationW pracy przedstawiono możliwości kształtowania plastycznego poprzez walcowanie, tłoczenie, gięcie, skręcanie blach wielowarstwowych zgrzewanych wybuchowo.
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Impedance analysis of Portevin-Le Chatelier effect on aluminium alloy
PublicationW niniejszej pracy przeprowadzono badania elektrochemiczne powierzchniowego efektu PLC, związanego z nierównomiernym procesem płynięcia plastycznego stopów aluminium zawierających magnez. Występujący efekt PLC w postaci serration wywołuje niehomogeniczny proces zmian zewnętrznej powierzchni rozciąganej próbki. Wykonano badania metodą Dynamicznej Spektroskopii Impedancyjnej w warunkach dynamicznych prowadzenia procesu rozciągania....
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The influence of PET mechanical properties on Stretch Blow Molding (SBM) process
PublicationIn the paper it is said about the influence of PET mechanical properties on SBM process parameters changes. The below paper mentions also about the influence of PET orientation and crystallization processes which have a very big influence on mechanical and thermal properties of PET material during SBM process. All mechanical data of PET material and SBM process parameters change in time are been got from collected literature which...
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SAMPLE-AND-HOLDING METHOD FOR GAS SENSING APPLIED FOR RESISTANCE GAS SENSORS
PublicationThe DC resistance of a Taguchi gas sensor is influenced by the ambient gas. To improve the selectivity and sensitivity of a sensor the fluctuation enhanced sensing (FES) was used. The paper proposes a novel methodology of utilizing FES technique that sample composition of an ambient atmosphere and hold it for further processing (sample-and-holding technique). The method uses the heated gas sensor to sample an ambient atmosphere...
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MP3vec: A Reusable Machine-Constructed Feature Representation for Protein Sequences
Publication—Machine Learning (ML) methods have been used with varying degrees of success on protein prediction tasks, with two inherent limitations. First, prediction performance often depends upon the features extracted from the proteins. Second, experimental data may be insufficient to construct reliable ML models. Here we introduce MP3vec, a transferable representation for protein sequences that is designed to be used specifically for sequence-to-sequence...