Wyniki wyszukiwania dla: BADANIA IN VITRO
-
Chicken feather keratin as a source of bioactive peptides useful in prevention of metabolic disorders – in silico and in vitro hydrolysis
PublikacjaProtein derived bioactive peptides not only serve as nutrients but can also exert drug-like activity, e.g. antidiabetic, antihypertensive, or antimicrobial, to name a few. Most biopeptides have beneficial effects on health that make them attractive for nutraceutical applications. The majority of bioactive peptides have been obtained from food proteins, but protein-rich by-products generated by agriculture...
-
Synthetic lipopeptides as potential topical therapeutics in wound and skin care: in vitro studies of permeation and skin cells behaviour
PublikacjaSeveral Gly-His-Lys analogues were obtained to investigate their antimicrobial properties. Three lipophilic analogues with the structure numbering 1b, 2b, 4b, exhibit significant effect against bacteria and were selected for in vitro evaluation of skin cells behaviour. In the present studies, an in vitro model of wound repair, proliferative cell staining, and tracking of living cells were used. Cell proliferation and cell migration,...
-
Effect of crystallinity on structural, thermal, and in vitro dissolution properties of Na2O-CaO-Nb2O5/MgO-P2O5 glass-ceramics
PublikacjaThe impact of the crystallinity on structural, thermal, and in vitro dissolution properties were examined for Na2O- CaO-Nb2O5/MgO-P2O5 glasses/glass-ceramics. Glass-ceramics were synthesized via a spontaneous crystallization process. The Nb content in the materials increased with melting temperature, furthermore, the crystallinity is proportional to the Nb content. The presence of crystalline niobates and phosphates is confirmed...
-
Electrochemistry meets enzymes: Investigation of the biotransformation pathway of C-1311 based on electrochemical simulation in comparison to in vitro methods.
PublikacjaThe knowledge of the metabolic pathways and the biotransformation of new drugs is one of the major challenges in pharmaceutical research. It is crucial for elucidation of degradation routes of the new biologically active compounds, especially in the area of possible toxicity. Conventional in vitro drug metabolism studies are based on incubating drug candidate with e.g. hepatocytes or, most importantly, liver cell microsomes and...
-
The Distribution of Glucosinolates in Different Phenotypes of Lepidium peruvianum and Their Role as Acetyl- and Butyrylcholinesterase Inhibitors—In Silico and In Vitro Studies
PublikacjaThe aim of the study was to present the fingerprint of different Lepidium peruvianum tu- ber extracts showing glucosinolates-containing substances possibly playing an important role in preventinting dementia and other memory disorders. Different phenotypes of Lepidium peruvianum (Brassicaceae) tubers were analysed for their glucosinolate profile using a liquid chromatograph coupled with mass spectrometer (HPLC-ESI-QTOF-MS/MS platform)....
-
A novel in vitro assay for assessing efficacy and toxicity of antifungals using human leukaemic cells infected with Candida albicans
PublikacjaAims: This study describes a novel in vitro assay that simultaneously determines antifungal efficiency and host cell toxicity using suspensions of human leukaemic cells (HL-60) infected with Candida albicans. Methods and Results: The effect of Candida infection on host cell viability was evaluated by the microscopy of trypan blue-stained cells and lactate dehydrogenase (LDH) activity. The in vitro ‘drug potency assay’ utilized...
-
Comet assay-based methods for measuring DNA repair in vitro; estimates of inter- and intra-individual variation.
PublikacjaZdolność do naprawy uszkodzeń DNA jest jednym z najważniejszych wyznaczników podatności na choroby nowotworowe. Dlatego też użytecznym staje się możliwość pomiaru stopnia naprawy DNA w próbkach materiału badawczego pobranego od ochotników. Celem pracy było, po pierwsze, opracowanie w warunkach in vitro prostej metody (z wykorzystaniem techniki testu kometowego), w której naprawa DNA odbywa się poprzez wycinanie nukleotydów (NER)....
-
Formulation and In Vitro Characterization of Bioactive Mesoporous Silica with Doxorubicin and Metronidazole Intended for Bone Treatment and Regeneration
Publikacja -
Correlation between physicochemical properties of doxorubicin-loaded silica/polydimethylsiloxane xerogel and in vitro release of drug
Publikacja -
Poly(amidoamine) Dendrimer/Camptothecin Complex: From Synthesis to In Vitro Cancer Cell Line Studies
Publikacja -
In vitro dissolution test of ketoprofene: development and evaluation of release from soft and hard gelatine capsules
Publikacja -
The effect of local anaesthetics on apoptosis and NETosis of human neutrophils in vitro: comparison between lidocaine and ropivacaine
Publikacja -
Spectral Characteristic and Preliminary Anticancer Activity in vitro of Selected Rhodanine‐3‐carboxylic Acids Derivatives
Publikacja -
Experimental immunology First report of cylindrospermopsin effect on human peripheral blood lymphocytes proliferation in vitro
Publikacja -
Expression Changes in Fatty acid Metabolic Processrelated Genes in Porcine Oocytes During in Vitro Maturation
Publikacja -
Dendrimer based theranostic nanostructures for combined chemo- and photothermal therapy of liver cancer cells in vitro
Publikacja -
“Bone Development” Is an Ontology Group Upregulated in Porcine Oocytes Before In Vitro Maturation: A Microarray Approach
Publikacja -
New markers for regulation of transcription and macromolecule metabolic process in porcine oocytes during in vitro maturation
Publikacja -
Expression and cellular distribution of zona pellucida glycoproteins in canine oocytes before and after in vitro maturation
Publikacja -
Antibacterial and antioxidant activity of the secondary metabolites from in vitro cultures of alice sundew (drosera aliciae)
PublikacjaRośliny owadożerne (D. aliciae) produkują metabolity wtórne, farmakologicznie czynne substancje. Na wzrost akumulacji tychże substancji w tkankach roślin owadożernych ma wpływ dodatek elicytora. W pracy przedstawiono zarówno optymalizacje warunków hodowli roślin, jak również prawdopodobne mechanizmy działania bakteriobójczego. Końcowego oznaczenia zawartości metabolitów w ekstraktach roślinnych dokonano techniką chromatografii...
-
Chemical Composition, In Vitro and In Situ Antimicrobial and Antibiofilm Activities of Syzygium aromaticum (Clove) Essential Oil
Publikacja -
Phenylalanine-Based AMPA Receptor Antagonist as the Anticonvulsant Agent with Neuroprotective Activity—In Vitro and In Vivo Studies
Publikacja -
Composition and In Vitro Effects of Cultivars of Humulus lupulus L. Hops on Cholinesterase Activity and Microbial Growth
Publikacja -
In vitro characteristics ofLactobacillusspp. strains isolated from the chicken digestive tract and their role in the inhibition ofCampylobactercolonization
Publikacja -
The new esters derivatives of betulin and betulinic acid in epidermoid squamous carcinoma treatment – In vitro studies
Publikacja -
In vitro to in vivo extrapolation to support the development of the next generation risk assessment (NGRA) strategy for nanomaterials
Publikacja -
Comparative in vitro study of single and four layer graphene oxide nanoflakes — Cytotoxicity and cellular uptake
Publikacja -
Using Flavonoid Substitution Status to Predict Anticancer Effects in Human Melanoma Cancers: An In Vitro Study
Publikacja -
In Vitro Screening for Anti-Acetylcholinesterase and Antioxidant Activities of Hottonia palustris L. Extracts and Their Unusual Flavonoids
Publikacja -
In Vitro Anticariogenic Effects of Drymocallis rupestris Extracts and Their Quality Evaluation by HPLC-DAD-MS3 Analysis
Publikacja -
The influence of extracts from Rubus caesius leaves on human colon carcinoma cells cultured in vitro
Publikacja -
In vitro anticariogenic effects of Rubus caesius extracts and their quality evaluation by HPLC-DAD-MS3 analysis
Publikacja -
Which casein in sodium caseinate is most resistant to in vitro digestion? Effect of emulsification and enzymatic structuring
PublikacjaWe investigated the resistance of individual constituent casein epitopes (αS1-, αS2-, β- and κ-CN) in food-grademilk protein sodium caseinate (NaCN) to simulated human gastro-duodenal digestion. The influence of NaCNadsorption to the surface of oil-in-water emulsion droplets and the effect of crosslinking of the protein withenzyme transglutaminase (TG) on the proteolysis were studied by indirect ELISA. TG crosslinking renderedfragments...
-
The influence of Nigella sativa essential oil on proliferation, activation, and apoptosis of human T lymphocytes in vitro
PublikacjaIn previous work, we tested the immunomodulatory effect of Nigella sativa (NS) fatty oil. Our results demonstrated that unrefined, obtained by cold pressing black cumin seed oil inhibited lymphocytes’ proliferation and induced their apoptosis in a dose-dependent manner. In this study, we examined the immunomodulatory properties of essential oil (EO) obtained from the NS seeds by hydrodistillation and its two main constituents:...
-
Influence of two cultivars of persimmon on atherosclerosis indices in rats fed cholesterol-containing diets: investigation in vitro and in vivo
PublikacjaCelem badań było oszacowanie wpływu dwóch odmian śliwy daktylowej na niektóre parametry arteriosklerozy u szczurów karmionych dietą zawierającą cholesterol. W ramach badań in vitro badano owoce dwóch odmian śliwy daktylowej jako dodatku do diety szczurów na zawartość związków bioaktywnych (polifenole, flawonoidy, flawonole, taniny, kartenoidy i kwas askorbinowy) i na aktywność przeciwutleniającą. W trakcie badań in vivo różnych...
-
In vitro studies of antimicrobial activity of Gly-His-Lys conjugates as potential and promising candidates for therapeutics in skin and tissue infections
PublikacjaWe presented in vitro studies of antimicrobial activity of Gly-His-Lys conjugates that are important point in preliminary biological evaluation of their potential application in skin and tissue therapy. The novel compounds include the conjugation of fatty acids with a modification of the amino acid sequence in the primary structure of Gly-His-Lys.
-
Antioxidant properties and bioactive constituents of some rare exotic thai fruits and comparison with conventional fruits. in vitro and in vivo studies
PublikacjaPrzedmiotem badań były ekstrakty uzyskane zarówno z egzotycznych owoców z terenu Tajlandii (durian, snake fruit, mangosteen) jak i owoców ''konwencjonalnych'' (mango, kiwi, awokado) w celu porównania zawartości związków bioaktywnych i aktywności przeciwutleniającej.Wyniki badań prowadzonych zarówno in vitro jak i in vivo mogą być wykorzystane przy opracowywaniu diety. W trakcie badań in vitro wykorzystano testy do określania potencjału...
-
Rational design, synthesis and biological evaluation of thiadiazinoacridines: a new class of antitumor agents.
PublikacjaZsyntetyzowano serię związków o potencjalnej zdolności do wiązania się z DNA z grupy tiadiazinoakrydyn poprzez cyklizację odpowiednich pochodnych 9-imino-4-nitro-9,10-dihydroakrydyny. Zbadano ich zdolność do wiązania się z DNA oraz cytotoksyczność względem kilku linii komórek nowotworwych. Badania aktywności przeciwnowotworowej wobec białaczki P388 wskazały na zgodność tych wyników z wynikami cytotoksyczności in vitro. Wykazano,...
-
In vitro affinity of Deinococcus radiodurans MutS towards mismatched DNA exceeds that of its orthologues from Escherichia coli and Thermus thermophilus
PublikacjaThe mismatch binding protein MutS is responsible for the recognition of mispaired and unpaired bases, which is the initial step in DNA repair. Among the MutS proteins most extensively studied in vitro are those derived from Thermus thermophilus, Thermus aquaticus and Escherichia coli. Here, we present the first report on the in vitro examination of DNA mismatch binding activity of MutS protein from Deinococcus radiodurans and confront...
-
[18F]Amylovis as a Potential PET Probe for β-Amyloid Plaque: Synthesis, In Silico, In vitro and In vivo Evaluations
Publikacja -
Zinc Oxide and Zinc Oxide Nanoparticles Impact on In Vitro Germination and Seedling Growth in Allium cepa L.
Publikacja -
Expression pattern of new genes regulating female sex differentiation and in vitro maturational status of oocytes in pigs
Publikacja -
Protein oligomerization is the biochemical process highly up-regulated in porcine oocytes before in vitro maturation (IVM)
Publikacja -
In vitro substance P-dependent induction of bone marrow cells in common (CD10) acute lymphoblastic leukaemia
Publikacja -
Chocolate desserts with ricotta hydrolysates: In vitro study of inhibitory activity against angiotensin‐converting enzyme and cholinesterase
Publikacja -
Application of bacterial cellulose experimental dressings saturated with gentamycin for management of bone biofilm in vitro and ex vivo
Publikacja -
In Vitro Evaluation of Polihexanide, Octenidine and NaClO/HClO-Based Antiseptics against Biofilm Formed by Wound Pathogens
Publikacja -
The processing of staphylococcal biofilm images from Fluorescence and Confocal Microscopy to assess in vitro efficacy of antiseptic molecules
Publikacja -
A Potential Relationship Between Estrogen Receptors Polymorphisms, Sperm Function and in vitro Fertilization Success: A Preliminary Study*
Publikacja -
GC-MS analysis, antidiabetic and antioxidant activity of methanolic extract of pluteus cervinus: an in vitro and in silico approach
Publikacja