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Rozmieszczenie wybranych metali ciężkich w ekosystemie oczyszczalni hydrofitowej w Bielkowie.Distribution of heavy metals in ecosystem of free water surface system in Bielkowo.
PublikacjaIn the article the results of investigations of biomass crop and heavy metal contents in bottom sediments and in three species of plants (Glyceria maxima, Typha latifolia)inhabiting a wetland system in Bielkowo, Poland are presented. The measurements were carried out three times during the vegetation season (May, July and October). The contents of metals in different fragments of plants (leaves, stalks and roots) was analyzed....
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Discriminating macromolecular interactions based on an impedimetric fingerprint supported by multivariate data analysis for rapid and label-free Escherichia coli recognition in human urine
PublikacjaThis manuscript presents a novel approach to address the challenges of electrode fouling and highly complex electrode nanoarchitecture, which are primary concerns for biosensors operating in real environments. The proposed approach utilizes multiparametric impedance discriminant analysis (MIDA) to obtain a fingerprint of the macromolecular interactions on flat glassy carbon surfaces, achieved through self-organized, drop-cast,...
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Strong Monogamies of No-Signaling Violations for Bipartite Correlation Bell Inequalities
PublikacjaThe phenomenon of monogamy of Bell inequality violations is interesting both from the fundamental perspective as well as in cryptographic applications such as the extraction of randomness and secret bits. In this article, we derive new and stronger monogamy relations for violations of Bell inequalities in general no-signaling theories. These relations are applicable to the class of binary output correlation inequalities known as...
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Versatile method employing basic techniques of genetic engineering to study the ability of low molecular weight compounds to bind covalently with DNA in cell free systems.
PublikacjaMechanizm działania większości leków przeciwnowotworowych oraz związków rakotwórczych polega na ich kowalencyjnym wiązaniu z DNA. Poziom tego wiązania jest zazwyczaj bardzo niski, co powoduje, że potrzebne są niezwykle czułe metody aby kowalencyjna modyfikacja mogła być w ogóle wykryta. My podjęliśmy próbę zastosowania w tym celu prostych, szybkich i sprawdzonych technik inżynierii genetycznej: metody PCR do uzyskania fragmentu...
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Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract
PublikacjaNumerous studies have shown, rather disappointingly, that isolated bioactive phytochemicals are not as biologically effective as natural plant products. Such a discrepancy may be explained by the concept of food synergy, which was verified in this research for cocoa extract versus its major components with regard to cancer chemoprevention. The evaluation embraced the relationship between redox properties evaluated in cell-free systems...
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Antibody reactivity in patients with IgE-mediated wheat allergy to various subunits and fractions of gluten and non-gluten proteins from ω-gliadin-free wheat genotypes
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Properties of dimension witnesses and their semidefinite programming relaxations
PublikacjaIn this paper we develop a method for investigating semi-device-independent randomness expansion protocols that was introduced in Li et al. [H.-W. Li, P. Mironowicz, M. Pawłowski, Z.-Q. Yin, Y.-C. Wu, S. Wang, W. Chen, H.-G. Hu, G.-C. Guo, and Z.-F. Han, Phys. Rev. A 87, 020302(R) (2013)]. This method allows us to lower bound, with semi-definite programming, the randomness obtained from random number generators based on dimension...
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Badania jakości docierania stali hartowanej docierakami aktywizowanymi w sposób swobodny = Study of hardened steel lapping quality where lapping tools are activized in a free way
PublikacjaPrzedstawiono badania jakości docierania stali hartowanej docierakami zbrojonymi w sposób swobodny. Analizowano wpływ podstawowych warunków obróbki na chropowatość powierzchni obrobionej. Wyznaczono zależność parametru Ra od prędkości i czasu docierania oraz nacisku jednostkowego. Analizowano również zanieczyszczenie powierzchni ścierniwem po docieraniu.
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Free Amino Acids and Biogenic Amines Content during Ageing of Dry-cured Pork Loins Inoculated with <i>Lactobacillus casei</i> ŁOCK 0900 Probiotic Strain
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Interference-Free Determination of Trace Copper in Freshly Ripened Honeys by Flame Atomic Absorption Spectrometry Following a Preconcentration by Solid-Phase Extraction and a Two-Step Elution Process
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Efficacy of rice husk biochar and compost amendments on the translocation, bioavailability, and heavy metals speciation in contaminated soil: Role of free radical production in maize (Zea mays L.)
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Surface-enhanced Raman scattering (SERS) and tip-enhanced Raman scattering (TERS) in label-free characterization of erythrocyte membranes and extracellular vesicles at the nano-scale and molecular level
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The Caucasian flora: a still-to-be-discovered rich source of antioxidants
PublikacjaCellular redox homeostasis is a state of balance between the formation of Usually Reactive Oxygen and / or Nitrogen Species (ROS/RNS), endogenous antioxidant defence systems, and exogenous dietary antioxidants. The disturbance of redox homeostasis, by the overproduction of endogenous ROS/RNS, may increase the risk of development of so-called civilisation diseases. The solution seems to be either the increased production of endogenous...
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Ceragenin CSA-13 as free molecules and attached to magnetic nanoparticle surfaces induce caspase-dependent apoptosis in human breast cancer cells via disruption of cell oxidative balance
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Qualitative analysis of phospholipids and their oxidised derivatives – used techniques and examples of their applications related to lipidomic research and food analysis
PublikacjaPhospholipids (PLs) are important biomolecules that not only constitute structural building blocks and scaffolds of cell and organelle membranes, but also play a vital role in cell biochemistry and physiology. Moreover, dietary exogenous PLs are characterized by high nutritional value and other beneficial health effects, which are confirmed by numerous epidemiological studies. For this reason, PLs are of high interest in lipidomics...
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Principles and applications of Ligation Mediated PCR methods for DNA-based typing of microbial organisms
PublikacjaA significant number of DNA-based techniques has been introduced into the field of microorganisms’ characterization and taxonomy. These genomic fingerprinting methods were developed to detect DNA sequence polymorphisms by using general principles, such as restriction endonuclease analysis, molecular hybridization, and PCR amplification. In recent years, some alternative techniques based on ligation of oligonucleotide adapters before...
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Amplification of erbB-4 oncogene occurs less frequently than that of erbB-2 in primary human breast cancer1Published in conjunction with A Wisconsin Gathering Honoring Waclaw Szybalski on the occasion of his 75th year and 20 years of Editorship-in-Chief of Gene, 10–11 August, 1997, University of Wisconsin, Madison, WI, USA.1
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Telomere uncapping by common oxidative guanine lesions: Insights from atomistic models
PublikacjaOxidative damage to DNA is widely known to contribute to aging and disease. This relationship has been extensively studied for telomeres – structures that cap chromosome ends – due to their role in cell proliferation and senescence, and exceptional susceptibility to oxidation. Indeed, the repetitive telomeric DNA sequence contains the 5′-GGG-3′ motif that has the lowest ionization potential of all trinucleotides. Accordingly, experiments...
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Jerzy Wtorek prof. dr hab. inż.
OsobyJerzy Wtorek uzyskał tytuł magistra inżyniera w 1976 roku na Wydziale Elektroniki Politechniki Gdańskiej, a stopień doktora nauk technicznych i doktora habilitowanego na Wydziale Elektroniki, Telekomunikacji i Informatyki Politechniki Gdańskiej, odpowiednio w 1986 i 2004 roku. Tytuł profesora uzyskał w 2016 r. Jego zainteresowania dotyczą nieinwazyjnych metod oceny układu krążenia, elektrycznych (aktywnych i pasywnych) właściwości...
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Kamila Kokot-Kanikuła mgr
OsobyKamila Kokot-Kanikuła pracuje w Bibliotece Politechniki Gdańskiej w Sekcji Budowy Zbiorów Cyfrowych i Multimedialnych na stanowisku starszego bibliotekarza. Jest absolwentką Instytutu Historycznego oraz Informacji Naukowej i Bibliotekoznawstwa na Uniwersytecie Wrocławskim. Główne kierunki zainteresowań to starodruki, biblioteki cyfrowe, repozytoria instytucjonalne, Otwarte Zasoby Edukacyjne, Open Access i Open Data. W bibliotece...
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Typing of Candida isolates from patients with invasive infection and concomitant colonization
PublikacjaWe investigated the relationship between colonizing and invasive isolates from patients with candidaemia. Molecular typing was performed using random amplification of polymorphic DNA (RAPD) and multilocus sequence typing (MLST). We found MLST to be sufficient for typing Candida isolates, and that surveillance cultures are helpful in predicting concomitant invasive isolates, but not necessarily the pathogen involved in subsequent...
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Increased concentration of Taq DNA polymerase as a solution for GC-rich templates from clinical and environmental samples
PublikacjaDNA polymerase is an enzyme which plays crucial role in replication and DNA repair. It found application in PCR (polymerase chain reaction) where catalyses process of in vitro DNA synthesis. To meet the demands posed by mod- ern diagnostic, molecular biology or genetic engineering it is necessary to improve DNA polymerases to obtain new or better features useful in these fields. So far implemented modifications in majority are...
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NASBA – IZOTERMICZNA AMPLIFIKACJA RNA
PublikacjaNASBA (ang. Nucleic Acid Sequence-Based Amplification) jest skuteczną i czułą techniką izotermicznej amplifikacji opartej na transkrypcji, służąca głównie do detekcji cząsteczek RNA. Znalazła ona zastosowanie do wykrywania RNA wirusowego, bakteryjnego i grzybiczego w próbkach klinicznych oraz żywości.
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The effect of anthraquinone derivatives on telomerase activity
Dane BadawczeThe data set contains images of the PCR products obtained from the Telomerase Repeated Amplification Protocol (TRAP). The effect of anthraquinone derivatives (TXT1-TXT9) on telomerase activity was comparatively examined using the following reference compounds: BIBR1532, TMPyP4 and mitoxantrone (MTX).
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The effect of anthraquinone derivatives (C1-C9) on telomerase activity
Dane BadawczeThe data set contains images of the PCR products obtained from the Telomerase Repeated Amplification Protocol (TRAP). The effect of anthraquinone derivatives (C-C9) on telomerase activity was analyzed using lysates from drug-treatedcancer cell line.
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Experience-based Intelligence Augmentation with Decisional DNA: Upcoming direction
PublikacjaIntelligence amplification systems and technologies have gained significant interest from academia and industry during the past few decades. One of the main reasons behind this trend is the fact that most experts agree that truly intelligent artificial system is yet to be developed. The question increasing often asked is this: Is full replication of human intelligence desirable key aim in intelligence related technology and research?...
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Michał Ryms dr inż.
Osobydr inż. Michał Ryms, fizyk, absolwent Politechniki Gdańskiej. Pracę doktorską obronił na Wydziale Chemicznym. Od 2011 roku pracuje w Katedrze Aparatury i Maszynoznawstwa Chemicznego Politechniki Gdańskiej, obecnie na stanowisku adiunkta. Działalność naukowa obejmuje: zagadnienia związane z poprawą efektywności energetycznej oraz możliwości nowego zastosowania materiałów zmiennofazowych (PCM) głównie w budownictwie energooszczędnym...
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Ewa Klugmann-Radziemska prof. dr hab.
OsobyEwa Klugmann-Radziemska ukończyła studia wyższe na kierunku fizyka na Uniwersytecie Gdańskim, a od roku 1996 związana jest z Politechniką Gdańską, kiedy to rozpoczęła studia doktoranckie. Obecnie jest profesorem na Wydziale Chemicznym Politechniki Gdańskiej, od roku 2006 kierownikiem Katedry Aparatury i Maszynoznawstwa Chemicznego. W latach 2008–2016 pełniła funkcję Prodziekana ds. współpracy i rozwoju, w latach 2016-2019 była...
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Badania charakterystyki obciążeń oddziałujących na modułowe urządzenie dylatacyjne w pasie ruchu ciężkiego autostrady A8
PublikacjaPrzedstawiono badania urządzenia dylatacyjnego typu nożycowego. Dokonano statycznych i dynamicznych pomiarów odkształceń oraz przemieszczeń konstrukcji nożyc na najbardziej obciążonym pasie autostrady A8. Określono wpływ oddziaływania dynamicznego oraz sporządzono histogramy obciążeń i współczynników przewyższenia dynamicznego na podstawie pomiarów ciągłych w okresie nasilonego ruchu. Wskazano przyczyny degradacji urządzenia. Odniesiono...
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A new assay for the simultaneous identification and differentiation of Klebsiella oxytoca strains.
PublikacjaKlebsiella oxytoca is the second most frequently identified species of Klebsiella isolated from hospitalized patients. Klebsiella spp. is difficult to identify using conventional methods and is often misclassified in clinical microbiology laboratories. K. oxytoca is responsible for an increasing number of multi-resistant infections in hospitals because of insufficient detection and identification. In this study, we propose a new...
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Joanna Czerska dr inż.
OsobyJoanna Czerska jestem człowiekiem, którego misją i pasją jest rozwój ludzi i organizacji. Moje motto to: „Nie ma takiej fantazji, której wola i rozum ludzki nie zdołałby przekształcić w rzeczywistość.” William Shakespeare W życiu kieruję się wartościami szacunku, pracy zespołowej i pozytywnego nastawienia. To one mnie definiują i decydują o tym jakim jest człowiekiem.Moja przygoda z Lean rozpoczęła się, podczas pisania pracy...
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FE analysis of a coupled energetic-statistical size effect in plain concrete beams with varying material properties.
PublikacjaThe numerical FE investigations of a coupled energetic-statistical size effect in unnotched concrete beams of similar geometry under quasi-static three point bending were performed within elasto-plasticity with non-local softening. The stochastic FE analyses were carried out with three different beam sizes. Deterministic calculations were performed with the uniform distribution of a uniaxial tensile strength. In statistical calculations...
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Development of the sound field 3D intensity probe based on miniature microphones
PublikacjaThe engineered measuring probe uses three pairs of miniature microphones coupled. The signals from the microphones after an initial amplification are fed to differential circuits. Due to the required symmetry of the circuit it was necessary to select electronic components very carefully. Moreover, additional digital signal processing techniques were applied to avoid amplitude and phase mismatch. The view of the engineered probe...
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Taq DNA polymerase fused with DNA binding protein with increased resistance to inhibitors from clinical samples
PublikacjaNowadays PCR method is commonly used in molecular diagnostic. However, in many cases PCR is limited, by the presence of inhibitory substances in biological, soil or food samples Efficiency and fidelity of amplification is strongly connected with DNA polymerase and reaction conditions. To meet the requirements of modern diagnostic methods it is essential to seeking for new DNA polymerases with better properties useful in these field....
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Janusz Smulko prof. dr hab. inż.
OsobyUrodził się 25 kwietnia 1964 r. w Kolnie. Ukończył w 1989 r. z wyróżnieniem Wydział Elektroniki Politechniki Gdańskiej, specjalność aparatura pomiarowa. Zajął II miejsce w konkursie Czerwonej Róży na najlepszego studenta Wybrzeża w 1989 r. Od początku kariery związany z Politechniką Gdańską: asystent (1989–1996), adiunkt (1996–2012), profesor nadzwyczajny PG (od 2012). Odbył staże naukowe w Texas A&M University (2003, NATO...
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Evaluation of possibilities of genotyping of Candida glabrata clinical isolates with RAPD-PCR method and microsatellite analysis
PublikacjaThe aim of the study was to compare the discriminatory power of RAPD-PCR method using RSD10 primer and microsatellite (GACA)4 analysis for genotyping clinical isolates of C. glabrata. Isolates were received from patients of two Polish hospitals: Children's Memorial Health Institute in Warsaw (n=17) and Medical University of Gdansk (n=37). Species identification was confirmed by two phenotypic methods: growth on chromagar plates...
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The New LM-PCR/Shifter Method for the Genotyping of Microorganism
PublikacjaTechniques relies on the ligation of appropriates adapters (LM-PCR) as AFLP, PCR MP and ADSRRS are successfully used for epidemiological studies for prokaryotic and eukaryotic microorganisms. In this study we propose a new method, called the LM-PCR/Shifter, based on the use of a Class IIS restriction enzyme giving restriction fragments with different 4 base 5' overhangs (Shifter) and the ligation of appropriate oligonucleotide...
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An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples
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Natural and synthetic antioxidants: An updated overview
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The onset of lipid peroxidation in rheumatoid arthritis: consequences and monitoring
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Diet is Not Responsible for the Presence of Several Oxidatively Damaged DNA Lesions in Mouse Urine
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Urinary excretion rates of 8-oxoGua and 8-oxodG and antioxidant vitamins level as a measure of oxidative status in healthy, full-term newborns
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8-Oxo-7,8-dihydroguanine and 8-oxo-7,8-dihydro-2′-deoxyguanosine levels in human urine do not depend on diet
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The plasma concentration of advanced oxidation protein products and arterial stiffness in apparently healthy adults
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Personalized nutrition in ageing society: redox control of major-age related diseases through the NutRedOx Network (COST Action CA16112)
PublikacjaA healthy ageing process is important when it is considered that one-third of the population of Europe is already over 50 years old, although there are regional variations. This proportion is likely to increase in the future, and maintenance of vitality at an older age is not only an important measure of the quality of life but also key to participation and productivity. So, the binomial “nutrition and ageing” has different aspects...
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A random signal generation method for microcontrollers with DACs
PublikacjaA new method of noise generation based on software implementation of a 7-bit LFSR based on a common polynomial PRBS7 using microcontrollers equipped with internal ADCs and DACs and a microcontroller noise generator structure are proposed in the paper. Two software applications implementing the method: written in ANSI C and based on the LUT technique and written in AVR Assembler are also proposed. In the method the ADC results are...
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ALIS-FLP: amplified ligation selected fragment-length polymorphism method for microbial genotyping
PublikacjaA DNA fingerprinting method known as ALIS-FLP (amplified ligation selected fragment-length polymorphism) has been developed for selective and specific amplification of restriction fragments from TspRI restriction endonuclease digested genomic DNA. The method is similar to AFLP, but differs in that only one specific restriction enzyme (TspRI) is used. The cohesive ends of the DNA fragments are ligated with two types of oligonucleotide....
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Recombinant !ermostable AP Exonuclease from Thermoanaerobacter tengcongensis: Cloning, Expression, Purification, Properties and PCR Application
PublikacjaApurinic/apyrimidinic (AP) sites in DNA are considered to be highly mutagenic and must be corrected to preserve genetic integrity, especially at high temperatures. !e gene encoding a homologue of AP exonuclease was cloned from the thermophilic anaerobic bacterium Thermoanaerobacter tengcongensis and transformed into Escherichia coli. The protein product showed high identity (80%) to human Ape1 nuclease, whereas to E. coli exonuclease...
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Analysis of pounding between adjacent buildings founded on different soil types
PublikacjaEarthquake-induced pounding was experienced in many previous earthquakes and it was found to be a critical issue. This study investigates the effect of pounding between buildings founded on the same and different soil types. Three 3-D buildings with 4, 6 and 8 storeys were considered in this study. Three pounding scenarios were taken into account, i.e. pounding between 4-storey and 6-storey buildings, between 4-storey and 8-storey...
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Jan Stąsiek prof. dr hab. inż.
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