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Wyniki wyszukiwania dla: EPITOPE MAPPING
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Epitope Mapping of BmpA and BBK32 Borrelia burgdorferi Sensu Stricto Antigens for the Design of Chimeric Proteins with Potential Diagnostic Value
PublikacjaLyme disease is a tick-borne zoonosis caused by Gramnegative bacteria belonging to the Borrelia burgdorferi sensu lato (s.l.) group. In this study, IgM- and IgG-specific linear epitopes of two B. burgdorferi sensu stricto (s.s.) antigens BmpA and BBK32 were mapped using a polypeptide array. Subsequently, two chimeric proteins BmpABBK32-M and BmpA-BBK32-G were designed to validate the construction of chimeras using the identified...
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In silico epitope prediction of Borrelia burgdorferi sensu lato antigens for the detection of specific antibodies
PublikacjaDespite many years of research, serodiagnosis of Lyme disease still faces many obstacles. Difficulties arise mainly due to the low degree of amino acid sequence conservation of the most immunogenic antigens among B. burgdorferi s.l. genospecies, as well as differences in protein production depending on the environment in which the spirochete is located. Mapping B-cell epitopes located on antigens allows for a better understanding...
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Chimeric Dr fimbriae with a Herpes Simplex virus type 1 epitope as a model for a recombinant vaccine.
PublikacjaBiałko adhezyny DraE uropatogennych szczepów E. coli Dr+ zostało wykorzystane jako nośnik 11a a determinanty antygenowej glikoproteiny D wirusa HSV ty-pu 1. Sekwencja kodująca epitop HSV została wklonowana do genu draE w obrębie regionu N- terminalnego eksponowanej powierzchniowo domeny 2. Ekspresjachimerycznych białek DraE-HSV została potwierdzona za pomocą mikroskopii immunofluorescencyjnej i elektronowej.
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Modeling of lapping plate wear and conditioning in single-sided lapping
PublikacjaIn order to achieve higher quality on worked surfaces with lower roughness, very high accuracy of shape and dimensions it is crucial to improve conventional finishing technologies and to develop new working principles. Single-sided lapping is one of the most effective planarization technologies and it is determined by a number of factors and boundary conditions. The most significant influence over a dimensional and profile accuracy,...
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Evaluating aerial laser scanning for seafloor mapping automation Shallow seabed mapping based on airborne Lidar bathymetry
PublikacjaThis article presents a novel methodological approach to understand and assess the suitability of ALB for the automatic classification and mapping of the seabed. ALB allows recording of the depth below the Secchi disk.
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Zero-Pole Space Mapping for CAD of Filters
PublikacjaIn this paper, we propose a new space-mapping technique tailored to the CAD of microwave filters. The goal of space mapping is to achieve a satisfactory design with the minimal number of fine model evaluations. In our approach, the filter is represented by a rational function. To quickly align the coarse and fine models, and to speed up the direct optimization of the coarse model, we propose matching the zeros and poles of a rational...
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Analysis of Mapping within S-module Framework
PublikacjaW artykule zaprezentowano wyniki prac na podejściem konglomeratowym. Podejście to polega na semantycznym opisywaniu zawartości modułów ontologicznych z wykorzystaniem zdefiniowanej algebry modułów. Głównym osiągnięciem zaprezentowanym w pracy jest opis procesu przekształcania bazy wiedzy DDL w zbiór konglomeratów. Jako że te dwie metody prezentują różne podejścia do modularyzacja, analiza procesu stanowi podstawę do dyskusji na...
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TIME SERIES DATA FOR 3D FLOOD MAPPING
PublikacjaThanks to the ability to collect information about large areas and with high frequency in time areas threatened by floods can be closely monitored. The effects of flooding are socio-economic losses. In order to reduce those losses, actions related to the determination of building zones are taken. Moreover, the conditions to be met by facilities approved for implementation in such areas are determined. Therefore, satellite data...
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Reverse vaccinology-based prediction of a multi-epitope SARS-CoV-2 vaccine and its tailoring to new coronavirus variants
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Reverse vaccinology-based prediction of a multi-epitope SARS-CoV-2 vaccine and its tailoring to new coronavirus variants
PublikacjaThe genome feature of SARS-CoV-2 leads the virus to mutate and creates new variants of concern. Tackling viral mutations is also an important challenge for the development of a new vaccine. Accordingly, in the present study, we undertook to identify B- and T-cell epitopes with immunogenic potential for eliciting responses to SARS-CoV-2, using computational approaches and its tailoring to coronavirus variants. A total of 47 novel...
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Expression of goose parvovirus whole VP3 protein and its epitopes in Escherichia coli cells
PublikacjaThe aim of this study was the expression of goose parvovirus capsid protein (VP3) and its epitopes in Escherichia coli cells. Expression of the whole VP3 protein provided an insufficient amount of protein. In contrast, the expression of two VP3 epitopes (VP3ep4, VP3ep6) in E. coli, resulted in very high expression levels. This may suggest that smaller parts of the GPV antigenic determinants are more efficiently expressed than the...
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Cell Wall Epitopes and Endoploidy as Reporters of Embryogenic Potential in Brachypodium Distachyon Callus Culture
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Mapping Invisibles - Acquiring GIS for Urban Planner Workshop
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Methods of mapping out the route contour of a railway line
PublikacjaStosowane obecnie toromierze elektroniczne wykorzystywane są przede wszystkim do pomiaru geometrii toru. Dane z pomiarów mogą być również wykorzystane w procesie projektowania regulacji toru (np. podczas usuwania dołków). W referacie przedstawiono metodę wyznaczania profilu podłużnego linii kolejowej na podstawie pomierzonych nierówności pionowych. Wyznaczony w ten sposób profil w dalszym etapie wykorzystany by był w projektowaniu...
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Capping or Suctioning for Tracheostomy Decannulation.
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Game with a Purpose for Mappings Verification
PublikacjaMappings verification is a laborious task. The paper presents a Game with a Purpose based system for verification of automatically generated mappings. General description of idea standing behind the games with the purpose is given. Description of TGame system, a 2D platform mobile game with verification process included in the gameplay, is provided. Additional mechanisms for anti-cheating, increasing player’s motivation and gathering...
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On homotopies of morphisms and admissible mappings
PublikacjaThe notion of homotopy in the category of morphisms introduced by G´orniewicz and Granas is proved to be equivalence relation which was not clear for years. Some simple properties are proved and a coincidence point index is described.
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Models of the kinematics of lapping machines.
PublikacjaPrzedstawiono wyniki analiz symulacyjnych układów kinematycznych docierania jednotarczowego powierzchni płaskich. Analizowano układ standardowy i układy niekonwencjonalne, zmieniając ich parametry geometryczne i kinematyczne. Wytypowano układ optymalny w aspekcie równomierności zużycia tarczy docierającej.
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Al203 sealing elements lapping
PublikacjaPrzedstawiono wyniki badań własnych wydajnosci docierania ceramiki Al203. Badano elementy uszczelniające na docierarce jednotarczowej o standardowym układzie kinematycznym. Określono wpływ wielkości mikroziaren węglika boru o numerze F400/17, F800/6.5 i F1200/3 oraz wartości nominalnego nacisku jednostkowego na ubytek materiału w czasie 15 i 20 min. Scharakteryzowano mechanizm usuwania naddatku przy docieraniu elementów ceramicznych....
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Analysis of abrasive powder at lapping
PublikacjaPrzedstawiono wyniki badań wpływu dawkowania pasty i zawiesiny ściernej na jakość docierania powierzchni płaskich. Analizowano parametry chropowatości powierzchni elementów ceramicznych i miedzianych.
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Real-Time Aerial Mapping by Image Features Extraction and Matching
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Value stream mapping as a tool to improve environmental management system
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Value stream mapping as a tool to improve environmental management system
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A novel architecture of Web-GIS for mapping and analysis of echinococcosis in Poland
PublikacjaEchinococcosis is an infectious disease transferred through ingestion of food or water which have been contaminated with eggs of the Echinococcus tapeworm, which are spread by intermediate parasite hosts. Because the latter are primarily territorial, research related to diagnosis and prevention of echinococcosis requires investigation of environmental factors, which can be supported with the use of a Geographical Information System...
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Automatic classification and mapping of the seabed using airborne LiDAR bathymetry
PublikacjaShallow coastal areas are among the most inhabited areas and are valuable for biodiversity, recreation and the economy. Due to climate change and sea level rise, sustainable management of coastal areas involves extensive exploration, monitoring, and protection. Current high-resolution remote sensing methods for monitoring these areas include bathymetric LiDAR. Therefore, this study presents a novel methodological approach to assess...
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Mapping knowledge risks: towards a better understanding of knowledge management
PublikacjaThis conceptual paper aims to identify, present, and analyze potential knowledge risks organizations might face. With the growing complexity of organizational environments and the plethora of new knowledge risks emerging, this critical but under-researched field of knowledge management (KM) deserves closer attention. The study is based on a critical analysis of the extant literature devoted to knowledge risks, discusses potential...
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Microgrinding with single-disk lapping kinematics.
PublikacjaGrinding operations are carried out with a variety of tool-workpiece configurations. The selection of a grinding process for a particular application depends on part shape, part size, ease of fixture, requirements concerning the acceptable shape errors. It is evident that lapping is very effective in eliminating the waviness while surface grinding is not. Dual disk machines for the double face grinding with planetary kinematics...
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Machining by abrasive-metallic lapping tools.
PublikacjaPrzedstawiono kontrukcję niekonwencjonalnych narzędzi ścierno-metalowych do docierania. Opracowane narzędzia wykorzystano do badań wydajności i jakości docierania elementów ceramicznych. Określono wpływ podstawowych warunków docierania na efekty obróbki powierzchni płaskich.
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Surfaces formability of PM parts by lapping.
PublikacjaPrzedstawiono wyniki badań docierania elementów spiekanych. Określono wpływ podstawowych warunków obróbki na jakość powierzchni płaskich oraz omówiono sposób wymuszonej aktywizacji powierzchni docieraka metalowego ścierniwem.
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Charging of the cast iron lapping tools.
PublikacjaPrzedstawiono technikę i technologię zbrojenia wymuszonego powierzchni docieraków tarczowych. Omówiono konstrukcję przyrządu oraz wyniki badań intensywności zbrojenia docieraków mikroziarnami węglika krzemu. Analizowano docieraki z żeliwa szarego i sferoidalnego oraz mikroziaren o numerze 320 i 300.
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Analysis of size abrasive micrograins in lapping
PublikacjaPrzedstawiono wyniki badań koncentracji i wielkości mikroziaren ściernych skażających powierzchnię żeliwa sferoidalnego podczas docierania. Podano wpływ współczynnika upakowania mikroziaren ściernych w strefie docierania na efekty skażenia powierzchni oraz zależności określające wpływ warunku procesu (nacisku jednostkowego, prędkości i czasu docierania) na parametry rozkładów wielkości mikroziaren ściernych.
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Measurement of surface flatness after lapping
PublikacjaPrzedstawiono sposób pomiaru płaskości powierzchni w docieraniu z wykorzystaniem metody sztucznych baz Vickersa. W analizie wykorzystano skomputeryzowane stanowisko do badań mikroskopowych, wyposażone w mikroskop stereoskopowy, kamerę CCD oraz program MultiScan.
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Localization of key amino acid residues in the dominant conformational epitopes on thyroid peroxidase recognized by mouse monoclonal antibodies
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Mapping the complexity of dementia: factors influencing cognitive function at the onset of dementia
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Substrate-integrated waveguide (SIW) filter design using space mapping
PublikacjaIn this paper, we present a fast technique for an automated design of microwave filters in substrate integrated wave (SIW) technology. The proposed methodology combines the space mapping technique with a cost function defined using the location of complex zeros and poles of filter’s transfer and reflection function and uses a rectangular waveguide as a surrogate model. The effectiveness of the proposed technique is presented with...
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Three-dimensional mapping for data collected using variable stereo baseline
PublikacjaThe paper describes a system of 3D mapping of data collected with due regard for variable baseline. This solution constitute an extension to a VisRobot sub-system developed as a subsystem, necessary for implementing the generic idea of using mobile robots to explore an indoor static environment. This subsystem is to acquire stereo images, calculate the depth in the images and construct the sought 3D map. Stereo images are obtained...
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Magnetic field mapping along a NV-rich nanodiamond-doped fiber
PublikacjaIntegration of NV−-rich diamond with optical fibers enables guiding quantum information on the spin state of the NV− color center. Diamond-functionalized optical fiber sensors have been demonstrated with impressive sub-nanotesla magnetic field sensitivities over localized magnetic field sources, but their potential for distributed sensing remains unexplored. The volumetric incorporation of diamonds into the optical fiber core allows...
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Mapping negative unintended consequences of disruptive technologies use in smart cities
PublikacjaSmart cities governance (SCG) consists of both to foster technology-enabled innovation, and to utilize disruptive technologies (DT) outcomes and impacts to increase public value of urban services. Despite widespread discussion of DT benefits, scientific literature identifies multiple determinants of unintended negative consequences (UC) of DT deployment in smart city initiatives. By considering UC as the negative aspects resulting...
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Introduction to smart solutions for wastewater: Road-mapping the transition to circular economy
PublikacjaToday there is an urgent need to move from a linear to a circular economy approach with special focus to water and environment. Despite the paramount importance, this transition is hampered by barriers which concern not only technological but embrace also organizational, regulatory, social, and economic aspects. In order to overcome such barriers, innovative water-smart solutions are needed and a robust roadmap for the transition...
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LiDAR based urban vegetation mapping as a basis of green infrastructure planning
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An automated microwave planar filter design based on space mapping optimization
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Mapping the spatial distribution of global anthropogenic mercury atmospheric emission inventories
PublikacjaW pracy przedstawiono procedury zastosowane do przygotowania map emisji rtęci ze źrodeł antropogennych na skale globalna i przedyskutowano wyniki obliczeń przedstawione na tych mapach. Dane o emisji użyte do przygotowania map zostały obliczone i opublikowane przez Pacyna et al. (2006). W niniejszej pracy przedstawiono mapy emsji dla roku 2000 oraz zmodyfikowane mapy emisji dla roku 1995.Mapy z rozkladem emisji w układzie gridów:...
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Landscape of Automated Log Analysis: A Systematic Literature Review and Mapping Study
PublikacjaLogging is a common practice in software engineering to provide insights into working systems. The main uses of log files have always been failure identification and root cause analysis. In recent years, novel applications of logging have emerged that benefit from automated analysis of log files, for example, real-time monitoring of system health, understanding users’ behavior, and extracting domain knowledge. Although nearly every...
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Depth Determination Accuracy of the Modified Prony Method in a Swath Mapping Application
PublikacjaThis article presents the performance of the modified Prony method in a swath mapping application. Depth determination accuracy is assessed by processing raw signal acquired by an EdgeTech 6205 swath bathymetry system over flat seafloor. An updated version of the method, proposed previously by the authors, is used to determine the number of signal echoes. The number of signal echoes is essential for performing the low-rank approximation...
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On the Nielsen fixed point theory for multivalued mappings
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The idea of a robotic single-sided lapping system
PublikacjaThis paper reviews the idea of a robotic lapping system. Robot’s assistance automates the lap-ping process and supports the development of a flexible lapping system. However, the main aim behind the idea of a robotic lapping system is to provide improved means for controlling the posi-tion of conditioning rings on a lapping plate. Due to the kinematics of lapping process, the profile wear of the tool is not constant along the radius....
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Research on the wear evenness of the tool in flat lapping
PublikacjaIn this paper, the dimensionless distribution of the material removal stock and the trajectories of abrasive grains cutting in single-sided lapping process are presented. They were numerically simulated based on the tribological model and kinematics equations. It can be observed that the trajectories geometry of single abrasive as well the velocities have a great influence on the final geometrical deviation. During machining, the...
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Influence of Flat Lapping Kinematics on Machinability of Ceramics
PublikacjaNew tools for flat grinding of ceramics are presented in the paper. Electroplated CBN tools (B64 and B107) were used in a modified single-disc lapping machine configuration. The results from experiments, such as the material removal rate and surface roughness parameters are presented and analyzed. Numerical simulations, based on the model for the shape error and tool wear estimation in machining with flat lapping kinematics, are...
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Shaping of the workpiece surface in single-disc lapping
PublikacjaShape errors of the tool exert a dominant influence on the shape accuracy of the workpiece. The correlation between flatness errors of the lap and flatness errors of the workpieces was checked experimentally and determined analytically. Computer model of the workpiece shaping by lapping was developed. Evaluation method of the workpiece orientation as well as some simulation results for a lap with shape errors of convexity and cincavity...
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Analysis of flat compensator lapping in an assembly process
PublikacjaPrzedstawiono sposób analizy docierania kompensatora płaskiego podczas montażu połączenia konstrukcyjnego. Wyznaczono czas główny obróbki i koszty operacji docierania jednotarczowego