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White light thermoplasmonic activated gold nanorod arrays enable the photo-thermal disinfection of medical tools from bacterial contamination
PublikacjaThe outspread of bacterial pathogens causing severe infections and spreading rapidly, especially among hospitalized patients, is worrying and represents a global public health issue. Current disinfection techniques are becoming insufficient to counteract the spread of these pathogens because they carry multiple antibiotic-resistance genes. For this reason, a constant need exists for new technological solutions that rely on physical...
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Dissection of σE‐dependent cell lysis in Escherichia coli: roles of RpoE regulators RseA, RseB and periplasmic folding catalyst PpiD
PublikacjaTo understand the mechanism of sigma(E)-dependent cell lysis, we examined the consequences of deletion derivatives of rpoE regulators rseA, rseB and rseC on sigma(E) transcription, on levels of free versus membrane-bound sigma(E) and on OMP-biogenesis limiting factor(s) that could impact cell lysis. RT-PCR showed that individual nonpolar DeltarseA and DeltarseB increased the rpoE expression to varying extents, with pronounced induction...
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Effect of Maximal Apnoea Easy-Going and Struggle Phases on Subarachnoid Width and Pial Artery Pulsation in Elite Breath-Hold Divers
PublikacjaPurpose: The aim of the study was to assess changes in subarachnoid space width (sas-TQ), the marker of intracranial pressure (ICP), pial artery pulsation (cc-TQ) and cardiac contribution to blood pressure (BP), cerebral blood flow velocity (CBFV) and cc-TQ oscillations throughout the maximal breath hold in elite apnoea divers. Non-invasive assessment of sas-TQ and cc-TQ became possible due to recently developed method based on...
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Synergic Effect of Naphthylated Carbon Nanotubes and Gold Nanoparticles on Catalytic Performance of Hybrid Films Containing Bilirubin Oxidase for the Dioxygen Reduction
PublikacjaTo improve the direct electron transfer from a multicopper oxidase MvBOd to the glassy carbon electrode surface the naphthylated multiwall carbon nanotubes (Napht-MWCNTs) are ombined with gold nanoparticles (AuNPs) to form a conductive network at the electrode and to increase its working surface. Such approach allowed to eliminate the necessity of employing mediators to reach elevated current values. The enzyme stability was improved...
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Visible light activity of pulsed layer deposited BiVO4/MnO2 films decorated with gold nanoparticles: The evidence for hydroxyl radicals formation
PublikacjaThin films containing BiVO4 and MnO2 deposited on FTO and modified by Au nanoparticles were studied towards their photoelectrochemical and photocatalytical activities in an aqueous electrolyte. Electrodes were prepared by the pulsed laser deposition (PLD) method. The surfactant-free ablation process was used for preparation of the gold nanoparticles (GNP) water suspension. Obtained layers of varied thicknesses (27–115 nm) were...
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In vitro affinity of Deinococcus radiodurans MutS towards mismatched DNA exceeds that of its orthologues from Escherichia coli and Thermus thermophilus
PublikacjaThe mismatch binding protein MutS is responsible for the recognition of mispaired and unpaired bases, which is the initial step in DNA repair. Among the MutS proteins most extensively studied in vitro are those derived from Thermus thermophilus, Thermus aquaticus and Escherichia coli. Here, we present the first report on the in vitro examination of DNA mismatch binding activity of MutS protein from Deinococcus radiodurans and confront...
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Technical fabrics old sample
Dane BadawczeThe data presented here includes video of technical fabric strength testing. The included movie is in .cine format, and an external program with a viewer for such movies is required.
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Interpreting the expansion of sea fishing in medieval Europe using stable isotope analysis of archaeological cod bones
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Determination of Chemical Oxygen Demand (COD) at Boron-doped Diamond (BDD) Sensor by Means of Amperometric Technique
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New and Old Genes Associated with Primary and Established Responses to Paclitaxel Treatment in Ovarian Cancer Cell Lines
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QEEG-based neural correlates of decision making in a well-trained eight-year-old chess player
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Non-specific signs of neuroinfection after a tick bite in a 10-year-old boy – a case report
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The influence of moderate pressure and subzero temperature on the shelf life of minced cod, salmon, pork and beef meat
PublikacjaThe effect of moderate pressure at subzero temperature on natural microflora of minced cod, salmony, pork and beef meat was studied. Pressure of 193 MPa at –20 °C caused reduction of total bacterial count in pork and beef meat by 1.1 and 0.6 log cycles, respectively, and by about 1.5 log cycles in fish meat. Under these conditions the psychrophilic and psychrotophic bacteria were below the detection limit (<10CFU/g of sample) in...
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Determination of Chemical Oxygen Demand (COD) at Boron-doped Diamond (BDD) Sensor by Means of Amperometric Technique
PublikacjaA boron-doped diamond (BDD) sensor was proposed for effective detection of chemical oxygen demand (COD) by means of amperometric technique. Thin boron doped diamond active sensor layer was deposited on Si wafer and glassy carbon substrate by Microwave Plasma Enhanced Chemical Vapor Deposition (MW PE CVD). The structure of BDD was confirmed by Raman spectra analysis. Broad Raman bands centered at 482 cm-1 and 1219 cm-1 are typical...
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Case Study of Old Steel Riveted Railway Truss Bridge: From Material Characterization to Structural Analysis
PublikacjaThe structural analysis of an old steel riveted railway truss bridge located over the Maruska River on the Działdowo – Olsztyn, Poland railway line is performed in this paper to check its behaviour under today’s railway loads. The mechanical properties of construction steel extracted from the old steel bridge are investigated by tensile tests, impact tests through the Charpy pendulum impact V-notch, and an optical emission spectrometer....
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Determination of chemical oxygen demand (cod) at boron-doped diamond (bdd) sensor by means of amperometric technique
PublikacjaA boron-doped diamond (BDD) sensor was proposed for effective detection of chemical oxygen demand (COD) by means of amperometric technique. Thin boron doped diamond active sensor layer was deposited on Si wafer and glassy carbon substrate by Microwave Plasma Enhanced Chemical Vapor Deposition (MW PE CVD). The structure of BDD was confirmed by Raman spectra analysis. Broad Raman bands centered at 482 cm-1 and 1219 cm-1 are typical...
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Application of hydroacoustic system of distance measurement for estimating the cod codends shape at the model research station in Insko
PublikacjaPraca przedstawia wyniki pomiarów kształtu worków dorszowych w stacji badań modelowych Politechniki Szczecińskiej w Ińsku. Badane worki dorszowe są workamispecjalnie zaprojektowanymi do połowów selektywnych. W celu precyzyjnego pomiaru parametrów geometrycznych worków został opracowany nowy hydroakustyczny system do precyzyjnych pomiarów odległości. Zapewnia on równoczesny pomiar maksymalnie 100 różnych wymiarów w zakresie od 0.1m...
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Voltage and frequency regulation of a standalone induction generator by reduced-rating power electronic compensators - comparative evaluation
PublikacjaIn this paper are considered two fundamental topologies of power electronic compensators for voltage and frequency control of a standalone self-excited induction generator (IG) system. These are voltage and current source inverter based shunt compensators with energy storage. The aim of this study is to assess main features of both topologies in dynamic and steady-state IG operation. The whole system is modelled with the aid of...
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Simulations of spacing of localized zones in reinforced concrete beams using elasto-plasticity and damage mechanics with non-local softening
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Applying ground penetrating radar to tracking of ancient architectural transformations: the case of the monastery St. Peter on the Island of Rab (Croatia)
PublikacjaThe ground-penetrating radar (GPR) method has been used for many years in archaeological research. However, thismethod is still not widely used in studies of past architecture. The biggest problem with the implementation of the GPRmethod at such sites is usually connected with extensive debris layers, plant cover and standing relics of walls and otherfeatures that restrict the available measurement area. Despite of these, properly...
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Left ventricular function after takotsubo is not fully recovered in long-term follow-up: A speckle tracking echocardiography study
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Exploring the impact of cultural context on eye-tracking studies of architectural monuments in selected European cities: Sustainable heritage management.
PublikacjaSustainable management of architectural heritage requires conducting an inclusive diagnosis of users’ opinions, considering both residents and tourists as the recipients of urban space. Given the cultural diversity within these groups, proposing the use of eye-trackers (ET) as an alternative to traditional public consultation prompts the need to assess the method’s advantages and disadvantages. It remains uncertain whether individuals...
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Heat transfer and pressure drop during flow boiling of pure efrigerants and refrigerant/oil mixtures in tube with porous coating
PublikacjaW pracy przedstawiono wpływ powłoki porowatej na średni współczynnik przejmowania ciepła oraz opory przepływu podczas wrzenia wybranych czynników chłodniczych wewnątrz poziomych rurek. Badania przeprowadzono dla techniczne czystych czynników chłodniczychR22, R134a i R407C oraz ich mieszanin z olejem poliestrowym 1% i 5% wrzących w rurkach wykonanych ze stali nierdzewnej, o wewnętrznej powierzchni gładkiej oraz z metaliczną powłoką...
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Active Annotation in Evaluating the Credibility of Web-Based Medical Information: Guidelines for Creating Training Data Sets for Machine Learning
PublikacjaMethods Results Discussion References Abbreviations Copyright Abstract Background: The spread of false medical information on the web is rapidly accelerating. Establishing the credibility of web-based medical information has become a pressing necessity. Machine learning offers a solution that, when properly deployed, can be an effective tool in fighting medical misinformation on the web. Objective: The aim of this study is to...
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Bioactive and biodegradable cotton fabrics produced via synergic effect of plant extracts and essential oils in chitosan coating system
PublikacjaFunctional antibacterial textile materials are in great demand in the medical sector. In this paper, we propose a facile, eco-friendly approach to the design of antibacterial biodegradable cotton fabrics. Cotton fiber fabrics were enhanced with a chitosan coating loaded with plant extracts and essential oils. We employed Fourier-transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS), UV–Vis spectrophotometry, optical...
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Creating private and public value in data-related management projects: a cross-border case study from Switzerland and Italy
PublikacjaThe literature in the field of smart cities shows a continuous emphasis and interest in the topic of big data due to the extensive use of Information and Communication Technologies by public and private institutions within each city. There is undoubtedly value in big data: in data lie insights on the city, its stakeholders, citizens, products, and services. Challenges, though, lie in data’s variety, volume, and velocity, but also...
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Simulations of spacing of localized zones in reinforced concrete beams using elastic-plasticity and damage mechanics with non-local softening
PublikacjaArtykuł omawia obliczanie belek żelbetowych z uwzględnieniem lokalizacji odkształceń. Obliczenia wykonano przy zastosowaniu MES na bazie sprężysto-plastycznego prawa konstytutywnego i modelu zniszczeniowego uwzględniającego degradację sztywności rozszerzonego o długość charakterystyczną mikrostruktury za pomocą teorii nielokalnej.
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Tracing the latest Jurassic–earliest Cretaceous paleoenvironment evolution in swell carbonate facies: a case study of the High-Tatric succession (Central Western Carpathians, Tatra Mts, Poland)
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Viability and intracellular nitric oxide generation in the umbilical cord blood CD34+CD133– and CD34+CD133+ cell populations exposed to local anaesthetics
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A five-year retrospective study shows increasing rates of antimicrobial drug resistance in Cabo Verde for both Staphylococcus aureus and Escherichia coli
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Escherichia coli Strains with Virulent Factors Typical for Uropathogens were Isolated from Sinuses from Patients with Chronic Rhinosinusitis—Case Report
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Comparison of the characteristics of gold nanoparticles synthesized using aqueous plant extracts and natural plant essential oils of Eucalyptus globulus and Rosmarinus officinalis
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Size-controlled synthesis of gold nanoparticles by a novel atmospheric pressure glow discharge system with a metallic pin electrode and a flowing liquid electrode
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Preparation and characterization of gold nanoparticles prepared with aqueous extracts of Lamiaceae plants and the effect of follow-up treatment with atmospheric pressure glow microdischarge
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Fatal sepsis in a pregnant woman with pyelonephritis caused by Escherichia coli bearing Dr and P adhesins: diagnosis based on postmortem strain genotyping
PublikacjaW pracy został opisany pierwszy śmiertelny przypadek młodej kobiety w ciąży z powodu przewlekłego zapalenia odmiedniczkowego nerek spowodowany przez szczepy E. coli z fimbriami typu Dr + i P. Badania pośmiertne wykazały, że śmierć nastąpiła z powodu posocznicy do której doszło na skutek zakażenia szczepami E. coli Dr + . Odmiedniczkowe zapalenie nerek w ciąży jest związane z wieloma powikłaniami, w tym ograniczeniem wzrostu płodu,...
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Electrochemical detection of bacterial endotoxin lipopolysaccharide (LPS) on gold electrode modified with DAL-PEG-DK5-PEG-OH - Antimicrobial peptide conjugate
PublikacjaThis work describes fabrication of gold electrodes modified with peptide conjugate DAL-PEG-DK5-PEG-OH that enables ultra-sensitive detection of lipopolysaccharide (LPS) isolated from the reference strain of Escherichia coli O26:B6. The initial step of the established procedure implies immobilization of the fully protected DAL-PEG-DK5-PEG-OH peptide on the surface of the gold electrode previously modified by cysteamine. Then side...
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Escherichia coli Strains with Virulent Factors Typical for Uropathogens were Isolated from Sinuses from Patients with Chronic Rhinosinusitis—Case Report
PublikacjaEscherichia coli were isolated from three patients with chronic rhinosinusitis (CRS) by intraoperative sinus tissue biopsy. Taking into account the unusual replicative niche and previous treatment failures, it was decided to focus on the virulence and drug resistance of these bacteria. The strains turned out to be multi-sensitive, but the rich virulence factors profile of bacteria typical for phylogenetic group B2 deserved attention....
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A five-year retrospective study shows increasing rates of antimicrobial drug resistance in Cabo Verde for both Staphylococcus aureus and Escherichia coli
PublikacjaObjectives Data on baseline drug resistance is important in informing future antimicrobial stewardship programs. So far, no data on the antimicrobial drug resistance of clinical isolates was available for the African archipelago of Cabo Verde. Methods We have performed a retrospective analysis over five-years (2013-17) of the antimicrombial drug susceptibility profiles of clinical isolates in the two main hospitals of Cabo Verde....
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A literature survey of the influence of preform reheating and stretch blow molding with hot mold process parameters on the properties of PET containers. Part I.
PublikacjaThe hot fill process is an inexpensive conventional filling technology for high-acidity products (pH < 4.5). It allows certain drinks (sensitive beverages such as fruit and vegetable juices, nectars, soft drinks, vitaminized water) to be stored at ambient temperature without the need for chemical preservatives. The primary feature of the bottles used in the hot fill process is their temperature stability, i.e. the ability to retain...
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Biofilm bakteryjny uropatogennych szczepów E. coli Dr+ jako czynnik indukujący przewlekłość zakażeń dróg moczowych ograniczający ich leczenie
PublikacjaZakażenia dróg moczowych (ZUM) stanowią jedne z najczęściej występujących infekcji bakteryjnych, dotykających każdego roku miliony osób na świecie. Problematyka tych zakażeń wynika z ich przewlekłości i nawrotów, pomimo stosowania terapii antybiotykowej oraz ciągle wzrastającej lekooporności uropatogenów je wywołujących. Dominującym czynnikiem etiologicznym ZUM są uropatogenne szczepy E. coli (UPECs), wykazujące zdolność do adhezji,...
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Synthesis of thiol derivatives of azobenzocrown ethers. The preliminary studies on recognition of alkali metal ions by gold nanoparticles functionalized with azobenzocrown and lipoic acid
PublikacjaThe article presents the synthesis of novel 13- and 16-membered azobenzocrown derivatives with peripheral thiol moieties and preliminary studies assessing their possible application in plasmonic sensors based on gold nanoparticles. The effect of the length of the chain connecting the macrocycle with the thiol group and the effect of the presence of the additional functional compound,...
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Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
PublikacjaThe RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional...
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Texture or Linker? Competitive Patterning of Receptor Assembly toward Ultra-Sensitive Impedimetric Detection of Viral Species at Gold-Nanotextured Titanium Surfaces
PublikacjaIn this work, we study the electrodes with a periodic matrix of gold particles pattered by titanium dimples and modified by 3-mercaptopropionic acid (MPA) followed by CD147 receptor grafting for specific impedimetric detection of SARS-CoV-2 viral spike proteins. The synergistic DFT and MM/MD modeling revealed that MPA adsorption geometries on the Au–Ti surface have preferential and stronger binding patterns through the carboxyl...
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Improvement of S355G10+N steel weldability in water environment by Temper Bead Welding
PublikacjaThe normalized S355G10 + N steel was chosen for testing, it is used in a variety of applications including the building of offshore structures, which may require repairs in water environment. The main aim of the work was to check susceptibility to cold cracking for fillet welds – Controlled Thermal Severity (CTS) tests and butt welds – Tekken tests and in the next step evaluation of effectiveness of Temper Bead Welding (TBW) application...
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Aneta Łuczkiewicz prof. dr hab. inż.
OsobyProwadzę badania nad zdolnościami adaptacyjnymi mikroorganizmów oraz możliwością ich wykorzystania do wspomagania procesów technologicznych. Łączę inżynierię środowiska z elementami inżynierii biomedycznej i materiałowej. Interesują mnie nowatorskie metody detekcji mikroorganizmów istotnych klinicznie w środowisku wodnym (tzw. epidemiologia oparta na ściekach) oraz zaawansowane i praktyczne rozwiązania w sektorze gospodarki wodnej....
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Ocena odporności na pękanie podbudów drogowych z mieszanek mineralno-cementowo-emulsyjnych (MCE)
PublikacjaPodbudowy z mieszanek mineralno-cementowo-emulsyjnych (MCE) są jednym z wielu materiałów stosowanych do wykonywania warstw nawierzchni w ramach przebudów dróg w Polsce. Mieszanki MCE pozwalają na maksymalne powtórne wykorzystanie istniejących warstw konstrukcyjnych do budowy lub przebudowy nawierzchni. Do wykonania podbudów z MCE stosuje się materiał pochodzący z rozbiórki istniejących warstw (destrukt) bitumicznych, warstw związanych...
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Novel single-stranded DNA-binding protein from psyvhrophilic bacterium Psychrobacter arcticus
PublikacjaTo study the biochemical properties of SSB from Psychrobacter arcticus (ParSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 642 nucleotides encoding SSB protein of 213 amino acids with a calculated molecular mass of 22.8 kDa. The amino-acid sequence of ParSSB exhibits 49% identity and 57% similarity to Escherichia coli SSB....
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Zagrożenia starych budynków w sąsiedztwie nowej zabudowy = The threat of old buildings security in neighbourhood of new buildings
PublikacjaNa przykładzie jednego z budynków mieszkalnych, przedstawiono zagrożenia na jakie są narażone stare obiekty w zwartej zabudowie śródmiejskiej, podczas prowadzenia w ich sąsiedztwie prac budowlanych, którym często towarzyszą głębokie wykopy. Powoduje to powstawanie licznych uszkodzeń, uniemozliwiających ich prawidłową eksploatację lub stanowiących nawet zagrożenie bezpieczeństwa.
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A concept of using static gps masurements for determination of vertical and horizontal land deformations in the main and old city of Gdańsk
PublikacjaArtykuł opisuje koncepcję wykorzystania statycznych pomiarów GPS (stacje GPS) przy analizie przemieszczeń gruntu na terenie Głównego i Starego Miasta w Gdańsku.
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Exploring the Phenomenon of the Additive Colour Process While Using a Computer Programme by 7–8-Year-Old Students
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