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Wyniki wyszukiwania dla: bee proteins
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Identification and properties of the Deinococcus grandis andDeinococcus proteolyticus single-stranded DNA binding proteins (SSB)
PublikacjaW celu poznania właściwości biochemicznych białek SSB pochodzących z Deinococcus grandis (DgrSSB) i Deinococcus proteolyticus (DprSSB) sklonowano zaamplifikowane w reakcji PCR geny ssb w systemie ekspresyjnym Escherichia coli. Geny składają się z 891(DgrSSB) i 876 (DprSSB) nukleotydów kodujących odpowiednio 296 i 291 reszt aminokwasowych o teoretycznie wyznaczonej masie cząsteczkowej dla białek, równej 32,29 i 31,33 kDa. Sekwencja...
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Bacterial SSB-like proteins of family Deinococcaceae - indentification, isolation, obtaining, purification and molecular characteristic
PublikacjaWiększość poznanych białek SSB jest aktywna w formie homotetramerycznej. Od 2002 roku znane są białka organizmów ekstremofilnych. Scharakteryzowano białka mikroorganizmów rodziny Deinococaceae. Uzyskane termostabilne białka stanowią atrakcyjną alternatywę w stosunku do znanych białek SSB w metodach diagnostyki molekularnej, technikach biologii molekularnej i analityce.
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Investigations of changes in the arabinogalactan proteins (AGPs) structure, size and composition during the fruit ripening process
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Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus - expression and purification
PublikacjaBiałka SSB są niezbędne do życia każdemu organizmowi. W tej pracy prezentujemy wyniki otrzymania i oczyszczania białek SSB Thermus thermophilus i T. aquaticus. Dotąd były znane białka SSB jedynie o budowie homotetrameru lub heterotrimeru. Białka SSB Thermus thermophilus i T. aquaticus stanowią nową klasę białek SSB - białek homodimerycznych. Ponadto dowiedziono, iż białka te są użyteczne w reakcji PCR.
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Unraveling the Interplay between DNA and Proteins: A Computational Exploration of Sequence and Structure-Specific Recognition Mechanisms
PublikacjaMy PhD dissertation focused on DNA-protein interactions and the recognition of specific DNA sequences and structures. I discovered that acidic amino acid residues (Asp/Glu) play a crucial role by exhibiting a preference for cytosine. Their contribution to binding affinity depends on nearby cytosines, balancing electrostatic repulsion with specific interactions. Acidic residues act as negative selectors, discouraging non-cytosine...
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Novel thermostable ssDNA-binding proteins from Thermus thermophilus and T. aquaticus-expression and purification
PublikacjaSklonowano w system ekspresyjny pET-30LIC Escherichia coli gen kodujący białka SSB-podobne Thermus thermophilus i T. aquaticus. Oczyszczono do homogenności białka SSB-podobne, a uzyskane preparaty umożliwiają wykonanie badań strukturalnych w/w białek.
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The Immunogenic and Immunoprotective Activities of Recombinant Chimeric T. gondii Proteins Containing AMA1 Antigen Fragments
PublikacjaToxoplasmosis, one of the most common parasitoses worldwide, is potentially dangerous for individuals with a weakened immune system, but specific immunoprophylaxis intended for humans is still lacking. Thus, efforts have been made to create an efficient universal vaccine for both animals and humans to overcome the shortcomings of currently used treatment methods and protect all hosts against toxoplasmosis. The current work represents...
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The biological role of prolyl oligopeptidase and the procognitive potential of its peptidic inhibitors from food proteins
PublikacjaProlyl oligopeptidase (POP) is a conserved serine protease belonging to proline-specific peptidases. It has both enzymatic and non-enzymatic activity and is involved in numerous biological processes in the human body, playing a role in e.g., cellular growth and differentiation, inflammation, as well as the development of some neurodegenerative and neuropsychiatric disorders. This article describes the physiological and pathological...
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Human sperm proteins identified by 2-dimensional electrophoresis and mass spectrometry and their relevance to a transcriptomic analysis
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Relationship between seawater pollution and qualitative changes in the extracted proteins from mussels Mytilus galloprovincialis
PublikacjaCelem badań było znalezienie wiarygodnego bioznacznika dla oceny poziomu zanieczyszczenia wody morskiej. W tym celu porównano:-zawartość żelaza (Fe) oraz miedzi (Cu)-białek-poziomu aktywności przeciwutleniającejmałży (Mytilus galloprovincialis)zebranych z rejonów zanieczyszczonych i niezanieczyszczonych bułgarskiej strefy ekonomicznej Morza Czarnego.W badanich wykorzystano takie techniki analityczne jak: -spektroskopia w podczerwieni...
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Probiotic strains from bee products - sequences and other data
Dane BadawczeThis folder contains seqences and other data necessary for evaluation of probiotic potential of bacteria islated from bee products.
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Antiviral activity of bee bread derived from polish apiaries.
Dane BadawczeBee bread is a product of fermentation of bee-collected pollen and revealed a high nutritional value. Other bee products, such as honey and propolis, are known for their antiviral activity, but bee bread is still under investigation, thus its antiviral potential is still unspecified. For investigation antiviral activity of bee bread samples, cytotoxicity...
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Probiotic properties of bacterial strains isolated form bee products.
Dane BadawczeThis folder contains information about probiotic properties of bacterial strains isolated from bee products. Additionally information about antibiotic resistance are included.
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Nitrogen mass balance and mathematical model of a Structured- Bed Reactor (SBRRIA)
PublikacjaAn up-flow structured-bed reactor subjected to recirculation and intermittent aeration (SBRRIA) was operated under varying influent conditions. The influent COD/N ratio was adjusted by maintaining a stable organic loading rate (average of 1.07 kgCOD m-3 d-1) and changing the nitrogen loading rate from 0.1 to 0.4 kg N m-3. Therefore, three different COD/N ratios were tested: 9.7±1 (Scenario 1); 7.6±1 (Scenario 2) and 2.9±1 (Scenario...
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A Test-Bed Analysis of Simultaneous PMIPv6 Handover in 802.11 WLANs Environment
PublikacjaProviding mobility in access networks is a challenge that we have to deal with. Due to networks’ convergence and migration to all-IP networks, mobility management at the network layer is required. However, there is a need for cooperation mechanisms between the network layer and lower layers to support multimedia services and make handover more efficient. This paper presents experimental research on simultaneous handover performance...
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The Numerical Comparison of Heat Transfer in a Coated and Fixed Bed of an Adsorption Chiller
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Optimization of a three-bed adsorption chiller by genetic algorithms and neural networks
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AMALIA REISENTHEL, „POLSKA TO KRAJ, W KTÓRYM ROZMAWIAM BEZ AKCENTU”
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Systemowa równowaga antyoksyacyjna u pacjentów bez klinicznej manifestacji miażdżycy
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Accuracy analysis of the RSSI BLE SensorTag signal for indoor localization purposes
PublikacjaIn this paper we describe possibility of use the RSSI signal (Radio Signal Strength Indication) from Texas Instruments SensorTag CC2650 for indoor positioning purposes. This idea is not a new but in our opinion it is possible to use SensorTags with Bluetooth LE wireless interface for positioning inside buildings in such applications as people findings in hospitals, senior come care, etc. RSSI is mostly selected as the sensor localization...
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CEE Trade in Services: Value-Added Versus Gross Terms Approaches
PublikacjaThis article assesses the impact of the determinants of service exports in both value-added terms and gross terms for seven Central Eastern European economies in 1995–2011. The results confirm the importance of increasing labor productivity and highly skilled and medium-skilled workers in the growth of trade in services. Exports of services are also supported by linkages between domestic services, especially business services,...
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Degradation of the Concrete Railway Track Bed Located in the Vicinity of the Loading Wharf
PublikacjaThe following article describes the damages of the concrete railway track bed at the place of unloading petroleum products located in the immediate vicinity of the cargo berth. The concrete of the track bed has been a subject to degradation as a result of many years of exploitation resulting, inter alia, in its cyclical flooding of petroleum products during the unloading and loading of rail tankers. Repairs carried out in the previous...
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Doskonalenie procesu produkcji farb proszkowych z wykorzystaniem wskaźnika OEE
PublikacjaW rozdziale omówiono problem poprawnego obliczania wskaźnika OEE w procesie produkcyjnym w przedsiębiorstwie X. Przeprowadzenie mapowania strumienia wartości, wywiadów z pracownikami, wykonanie analiz z wykorzystaniem testów statystycznych danych archiwalnych oraz wartości parametrów technologicznych zmierzonych w trakcie pracy linii umożliwiły opracowanie nowej formuły wyznaczania OEE. To z kolei pozwoliło na dokładne określenie...
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Choosing source of microorganisms and processing technology for next generation beet bioinoculant
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Efficient analysis of waveguide componets using a hybrid PEE-FDFD algorithm.
PublikacjaZaproponowano przyspieszenie analizy podzespołów falowodowych poprzez połączenie metody różnic skończonych w dziedzinie częstotliwości FDFD oraz rozwinięcia w funkcje własne PEE. Proponowane sformułowanie pozwala jawnie zdefiniować operator macierzowy dla zadanego problemu. Proponowana technika została zaprezentowana zarówno dla problemów własnych jak i układów z pobudzeniem.
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Tragfähigkeitsminderung durch lokale Instabilität bei stählernen Zylindertanks mit Doppelmantel
PublikacjaOmówiono zagrożenie stateczności i nosności płaszczy i den zbiorników dwupłaszczowych w tym także zbiorników na ciecze magazynowe w wysokiej temperaturze (asfalt magazynowany w temparaturze +200 C. Rozważania teoretyczne odniesiono do sytuacji awaryjnych które wystąpiły w praktyce.
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Standard and Fast Selective Catalytic Reduction of NO with NH3on Zeolites Fe-BEA
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Irrigation Effects of Red Beet as Affected by Rainfall in Different Regions of Poland
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How to define and measure informal employment in CEE countries - the case of Poland
PublikacjaThe study of informal employment is still associated with several obstacles, from the scope of definition, through measurement methods, to policy recommendations. This article aims to revise the existing methodological frames in order to point out how to improve the study of informal employment in CEE countries. The case of Poland serves to examine whether the common definitions and measurement methods are suitable for an inclusive...
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Obraz olejny, bez tytułu, 100 cm. x 140 cm.
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Obraz olejny, bez tytułu, 120 cm x 180 cm.
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Research of Accuracy of RSSI Fingerprint-Based Indoor Positioning BLE System
PublikacjaRadio localization in indoor environment is still a challenging task due to environment volatility. In the paper are compared achieved localization accuracies for RSSI-Fingerprinting method utilizing Bluetooth Low Energy (BLE) for two different environments: large empty hall and narrow corridor. Measurements were done by 6 different smartphones of 3 different producers, which makes those measurements unique as accuracies achieved...
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Technique for reducing erosion in large-scale circulating fluidized bed units
PublikacjaThis paper presents a methodology, implemented for a real industrial-scale circulating fluidized bed boiler, to mitigate the risk of heating surfaces exposed to an intensive particle erosion process. For this purpose, a machine learning algorithm was developed to support the boiler reliability management process. Having a tool that can help mitigate the risk of uncontrolled power unit failure without expensive and technically complex...
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Research of Accuracy of RSSI Fingerprint-Based Indoor Positioning BLE System
PublikacjaRadio localization in indoor environment is still a challenging task due to environment volatility. In the paper are compared achieved localization accuracies for RSSI-Fingerprinting method utilizing Bluetooth Low Energy (BLE) for two different environments: large empty hall and narrow corridor. Measurements were done by 6 different smartphones of 3 different producers, which makes those measurements unique as accuracies achieved...
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Bees Detection on Images: Study of Different Color Models for Neural Networks
PublikacjaThis paper presents an approach to bee detection in video streams using a neural network classifier. We describe the motivation for our research and the methodology of data acquisition. The main contribution to this work is a comparison of different color models used as an input format for a feedforward convolutional architecture applied to bee detection. The detection process has is based on a neural binary classifier that classifies...
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Stability of delay induced oscillations in gene expression of Hes1 protein model
PublikacjaW pracy prezentowana jest matematyczna analiza modelu ekspresji genu białka Hes1. Rozważamy układ równań z dwoma opóźnieniami - w produkcji białka Hes1 oraz jego mRNA. Dowodzimy istnienia i jednoznaczności oraz nieujemności rozwiązań. Pokazujemy istnienie dokładnie jednego dodatniego stanu stacjonarnego. Wykazujemy, że stabilność stanu stacjonarnego zależy od sumy opóźnień. Podajemy warunek dostateczny na wystąpienie bifurkacji...
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Novel primosomal protein B from thermophilic bacterium Thermoanerobacter tengcongensis
PublikacjaWe report the identification and characterization of theprimosomal protein B (PriB) from thermophilic bacteriumThermoanerobacter tengcongensis (TtePriB). It is the largestknown bacterial PriB protein consisting 216 amino acidresidues with a calculated molecular mass of 25 kDa. Surprisingly,it is functional as monomer containing two single-stranded DNA binding domain (OB-fold) and it is thecompletely new kind structure of SSB protein....
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Functional Protein Composition in Femoral Glands of Sand Lizards (Lacerta agilis)
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Zeta potential and droplet size of n-tetradecane/ethanol (protein) emulsions
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Two-dimensional gel-based serum protein profile of growing piglets
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CAVER 3.0: A Tool for the Analysis of Transport Pathways in Dynamic Protein Structures
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BALCONY: an R package for MSA and functional compartments of protein variability analysis
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Myotilin, a New Topotecan Resistant Protein in Ovarian Cancer Cell Lines
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C-reactive protein as a diagnostic and prognostic factor in inflammatory bowel diseases
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Simulation of Protein Structure and Dynamics with the Coarse-Grained UNRES Force Field
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Inhibition of protein glycosylation reverses the MDR phenotype of cancer cell lines
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Blind prediction of homo‐ and hetero‐protein complexes: The CASP13‐CAPRI experiment
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Water-mediated influence of a crowded environment on internal vibrations of a protein molecule
PublikacjaThe influence of crowding on the protein inner dynamics is examined by putting a single protein molecule close to one or two neighboring protein molecules. The presence of additional molecules influences the amplitudes of protein fluctuations. Also, a weak dynamical coupling of collective velocities of surface atoms of proteins separated by a layer of water is detected. The possible mechanisms of these phenomena are described....
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Trial Proteomic Qualitative and Quantitative Analysis of the Protein Matrix of Submandibular Sialoliths
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Riboflavin binding protein—Chiral stationary phase: Investigation of retention mechanism
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