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Cloning and characterization of a novel cold-active glycoside hydrolase family 1 enzyme with beta-glucosidase, beta-fucosidase and beta-galactosidase activities.
PublikacjaBackground: Cold-active enzymes, sourced from cold-adapted organisms, are characterized by high catalytic efficiencies at low temperatures compared with their mesophilic counterparts, which have poor activity. This property makes them advantageous for biotechnology applications as it: (i) saves energy costs, (ii) shortens the times for processes operated at low temperatures, (iii) protects thermosensitive substrates or products...
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A new cold-adapted beta-D-galactosidase from the Antarctic Arthrobacter sp. 32c - gene cloning, overexpression, purification and properties
PublikacjaThe development of a new cold-active β-D-galactosidases and microorganisms that efficiently ferment lactose is of high biotechnological interest, particularly for lactose removal in milk and dairy products at low temperatures and for cheese whey bioremediation processes with simultaneous bio-ethanol production. In this article, we present a new β-D-galactosidase as a candidate to be applied in the above mentioned biotechnological...
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Transcriptome profiling reveals distinctive traits of retinol metabolism and neonatal parallels in the MRL/MpJ mouse
PublikacjaBackground: The MRL/MpJ mouse is a laboratory inbred strain known for regenerative abilities which are manifested by scarless closure of ear pinna punch holes. Enhanced healing responses have been reported in other organs. A remarkable feature of the strain is that the adult MRL/MpJ mouse retains several embryonic biochemical characteristics, including increased expression of stem cell markers. Results: We explored the transcriptome...
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Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, and Photobacterium profundum
PublikacjaBackground: Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination and repair in Bacteria, Archaea and Eukarya. In recent years, there has been an increasing interest in SSBs, since they find numerous applications in diverse molecular biology and analytical methods. Results: We report the characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea...
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Drug-resistant and hospital-associated Enterococcus faecium from wastewater, riverine estuary and anthropogenically impacted marine catchment basin.
PublikacjaEnterococci, ubiquitous colonizers of humans and other animals, play an increasingly important role in health-care associated infections (HAIs). Acquisition of resistance determinants not only seriously limits available therapeutic options but also increases available gene pool for other species. It is believed that the recent evolution of two clinically relevant species, Enterococcus faecalis and Enterococcus faecium occurred...
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Neotenic phenomenon in gene expression in the skin of Foxn1- deficient (nude) mice - a projection for regenerative skin wound healing
PublikacjaMouse fetuses up to 16 day of embryonic development and nude (Foxn1- deficient) mice are examples of animals that undergo regenerative (scar-free) skin healing. The expression of transcription factor Foxn1 in the epidermis of mouse fetuses begins at embryonic day 16.5 which coincides with the transition point from scar-free to scar-forming skin wound healing. In the present study, we tested the hypothesis that Foxn1 expression...
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The usefulness of biotyping in the determination of selected pathogenicity determinants in Streptococcus mutans
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A prototypical non-malignant epithelial model to study genome dynamics and concurrently monitor micro-RNAs and proteins in situ during oncogene-induced senescence
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Expression-based decision tree model reveals distinct microRNA expression pattern in pediatric neuronal and mixed neuronal-glial tumors
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Correction to: A prototypical non-malignant epithelial model to study genome dynamics and concurrently monitor micro-RNAs and proteins in situ during oncogene-induced senescence
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Transcriptomic responses to wounding: meta-analysis of gene expression microarray data
PublikacjaBackground A vast amount of microarray data on transcriptomic response to injury has been collected so far. We designed the analysis in order to identify the genes displaying significant changes in expression after wounding in different organisms and tissues. This meta-analysis is the first study to compare gene expression profiles in response to wounding in as different tissues as heart, liver, skin, bones, and spinal cord, and...
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Fibronectin-, vitronectin- and laminin-binding proteins at the cell walls of Candida parapsilosis and Candida tropicalis pathogenic yeasts
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Expression of alternatively spliced variants of the Dclk1 gene is regulated by psychotropic drugs
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A qualitative 5-country comparison of the perceived impacts of COVID-19 on people living with dementia and unpaid carers
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Comparison of the adaptive implementation and evaluation of the Meeting Centers Support Program for people with dementia and their family carers in Europe; study protocol of the MEETINGDEM project
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Mapping the complexity of dementia: factors influencing cognitive function at the onset of dementia
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Effect of renal replacement therapy on selected arachidonic acid derivatives concentration
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Xanthine oxidoreductase activity in platelet-poor and rich plasma as a oxidative stress indicator in patients required renal replacement therapy
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Characterization of exceptionally thermostable single-stranded DNA-binding proteins from Thermotoga maritima and Thermotoga neapolitana
PublikacjaBACKGROUND: In recent years, there has been an increasing interest in SSBs because they find numerous applications in diverse molecular biology and analytical methods.RESULTS: We report the characterization of single-stranded DNA binding proteins (SSBs) from the thermophilic bacteria Thermotoga maritima (TmaSSB) and Thermotoga neapolitana (TneSSB). They are the smallest known bacterial SSB proteins, consisting of 141 and 142 amino...
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Changes in gene methylation patterns in neonatal murine hearts: Implications for the regenerative potential
PublikacjaBackground The neonatal murine heart is able to regenerate after severe injury; this capacity however, quickly diminishes and it is lost within the first week of life. DNA methylation is an epigenetic mechanism which plays a crucial role in development and gene expression regulation. Under investigation here are the changes in DNA methylation and gene expression patterns which accompany the loss of regenerative potential. Results The...
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Microplastics removal from aqueous environment by metal organic frameworks
PublikacjaThis paper provides an overview of recent research performed on the applications of metal–organic frameworks (MOFs) for microplastics (MPs) removal from aqueous environments. MPs pollution has become a major environmental concern due to its negative impacts on aquatic ecosystems and human health. Therefore, developing effective and sustainable methods for removing them from aqueous environments is crucial. In recent years, MOFs...
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An automated, low-latency environment for studying the neural basis of behavior in freely moving rats
PublikacjaBackground Behavior consists of the interaction between an organism and its environment, and is controlled by the brain. Brain activity varies at sub-second time scales, but behavioral measures are usually coarse (often consisting of only binary trial outcomes). Results To overcome this mismatch, we developed the Rat Interactive Foraging Facility (RIFF): a programmable interactive arena for freely moving rats with multiple feeding...
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Chromium‑based metal organic framework for pipette tip micro‑solid phase extraction: an effective approach for determination of methyl and propyl parabens in wastewater and shampoo samples
PublikacjaAbstract Background A chromium-based metal organic framework was synthesized and employed as an efficient sorbent for pipette tip micro-solid phase extraction and preconcentration of parabens from wastewater and shampoo samples up to sub-ppb level before their spectrophotometric analysis. Results Factors affecting preconcentration including volume and type of solvent, amount of sorbent, number of extraction, and volume and pH of...
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Application of a novel deep eutectic solvent modified carbon nanotube for pipette-tip micro solid phase extraction of 6-mercaptopurine
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Assessment of antibiotic resistance and biofilm formation of Enterococcus species isolated from different pig farm environments in Poland
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Does obesity rule out happiness? Preschool children’s perceptions of beauty-related happiness
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EUMaster4HPC Europejskie konsorcjum na rzecz edukacji w HPC
ProjektyProjekt realizowany w Katedra Architektury Systemów Komputerowych
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Implementation of Hierarchical Control of Drinking Water Supply System
PublikacjaThe paper presents the outline of the didactical project of a complex computer controlled system realized by the first degree students of the Automatics and Robotics on the Faculty of Electrical and Control Engineering (FoEaCE) in Gdansk University of Technology (GUT). The synthesis, implementation and analysis of a multilayer hierarchical control system for drinking water supply system (DWSS) are main topics of that project. The...
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Urban flash flood hazard identification and assessment applying geospatial techniques and hydrodynamic modeling; Erbil city case study, Kurdistan Region of Iraq
PublikacjaThis dissertation aims to investigate the factors behind flash flooding in Erbil's central district, located in the Kurdistan Region of Iraq, and develop a methodology for assessing flood hazards in the city, despite limited data accessibility. In this thesis, each factor was investigated, including analyzing extreme precipitation events in the last two decades, including their spatial and temporal distribution of rainfall, intensity,...
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User-assisted methodology targeted for building structure interpretable QSPR models for boosting CO2 capture with ionic liquids
PublikacjaTask-specific ionic liquid (IL) is an emerging class of compounds that may be environmentally friendly. Properly selected, these compounds may be green alternative to amine solutions and can replace them in post-combustion carbon dioxide (CO2) capture processes on an industrial scale. However, owing to the vast diversity of ions and their possible combinations, laboratory research is time consuming and expensive. Therefore, computational...
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A New Approach to Assess Quality of Motion in Functional Task of Upper Limb in Duchenne Muscular Dystrophy
Publikacja(1) Background: This study presents a new method for the motion quantitative analysis of Duchenne muscular dystrophy patients (DMD) performing functional tasks in clinical conditions. (2) Methods: An experimental study was designed to define how different levels of external mass (light and heavy) influence the performance of the upper limbs of a tested DMD and reference subject (RS) during horizontal movements (level of the waist)...
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Genistein: a lysosomal stimulator for treatment of various lysosomal diseases
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Uniwersalny system bezprzewodowy do monitorowania zagrożeń bezpieczeństwa
PublikacjaW pracy przedstawiono budowę i działanie bezprzewodowego systemu monitorowania zagrożeń bezpieczeństwa, opartego na koncepcji samoorganizującej się sieci uniwersalnych węzłów telekomunikacyjnych (UWT). Na wstępie opisano schemat funkcjonalny tego systemu, uwzględniając szczególnie wynikające z tego zadania i budowę UWT. Następnie na wybranym przykładzie scharakteryzowano pracę samoorganizującej się sieci UWT - sieci ad-hoc. W końcowej...
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Analysis and comparision of safety of children and adult passanger in car based on crash tests results
PublikacjaThis paper presents analysis and comparision of safety of children and adult passanger in car based on crash tests results. The car crash test with four dummies, including two car seats allowed a direct comparison of the security under the same conditions of children and passengers directly to the car seat. Analysis of acceleration and video from the test allows to understand the phenomena during the collision, the air bags influence,...
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Specyfika badań kompatybilności elektromagnetycznej w zakładowych laboratoriach badawczych
PublikacjaW artykule przedstawiono metodykę postępowania stosowaną przy rozwiązywaniu zagadnień związanych z badaniami kompatybilności elektromagnetycznej (EMC) wykonywanymi w zakładowych laboratoriach badawczych. Przedstawiono podstawowe różnice w metodyce prowadzonych badań powstające między laboratoriami zakładowymi a zewnętrznymi jednostkami akredytowanymi. Odstępstwa od reguł przewidzianych w odnośnych normach wynikają głównej z faktu,...
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Electrochemical Activity of Electrode Material Consisting of Porous Copper and Silica Aerogel
PublikacjaElectrochemically obtained copper was combined with silica aerogel (SiO2ag). Obtained composite (SiO2@Cu/CuxOy) was tested as an electrode in contact with LiPF6 in EC/DMC electrolyte. The electrochemical measurements showed the presence of complex redox couple activities of SiO2@Cu/CuxOy. This experiment was employed to help in understanding the carbon role used as an electric contact for silica in Li-Ion batteries and clarify...
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Zmodyfikowana metoda prognozowania rezultatów nadzorowania drgań narzędzie-przedmiot obrabiany.
PublikacjaW pracy przedstawiono zmodyfikowaną metodę prognozowania rezultatów nadzorowania drgań narzędzie-przedmiot obrabiany za pomocą sterowanej programowo prędkości obrotowej wrzeciona. Istotą modyfikacji jest uwzględnienie w modelu złożoności geometrycznej narzędzia oraz efektu rozproszenia energii procesu skrawania. Podano opis dynamiki skrawania niestacjonarnego modelu obliczeniowego, oraz sposobu nadzorowania. Zamieszczono przykład...
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Ocena zgodności urządzeń z dyrektywą kompatybilności elektromagnetycznej. '' Edukacyjny program Jakości zasilania Leonardo''. Leonardo Power Quality Initiative.
PublikacjaCelem publikacji jest przedstawienie procedury oceny wyrobów podlegających dyrektywie EMC. Należy zaznaczyć, że zgodnie z prawem europejskim tryb oceny wyrobu dla poszczególnych dyrektyw jest odmienny i wynika z szczegółowych rozporządzeń ( Rozporządzenie Rady Ministrów z dnia 25 czerwca 2002 r. w sprawie warunków i trybu dokonywania oceny zgodności z zasadniczymi wymaganiami dotyczącymi kompatybilności elektromagnetycznej...
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System reputacyjny z centralnym agentem i metrykami zaufania opartymi na poziomie świadczonych usług sieciowych
PublikacjaZaproponowano nowy system reputacyjny zdolny do identyfikacji egoistycznych węzłów w wieloskokowych sieciach bezprzewodowych ad hoc. Zawiera on mechanizmy oceny stopnia kooperatyw-ności węzłów oraz wymuszania współpracy, sprawiając, że zachowania egoistyczne pogarszają relację wydajności i kosztów dla węzłów. Podejście opiera się na historycznych wartościach reputacji i zachowań węzłów, heurystycznym algorytmie identy-fikacji...
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High Frequency Harmonics Emission in Smart Grids
PublikacjaPulse-width modulated rectifiers are nowadays commonly used for AC to DC power conversion. PWM rectification technology is very effective and allows for bidirectional power flow with the possibility of power factor improvement and low-order harmonic emission limitation. Unfortunately, employed PWM boost topology results with generation of input current harmonic distortions in a frequency range tightly correlated to modulation frequency....
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Low Frequency Conducted Emissions of Grid Connected Static Converters
PublikacjaPulse-width modulated rectifiers are nowadays commonly used for AC to DC power conversion. PWM rectification technology is very effective and allows for bidirectional power flow with the possibility of power factor improvement and low-order harmonic emission limitation. Unfortunately, employed PWM boost topology results with generation of input current harmonic distortions in a frequency range tightly correlated to modulation frequency....
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Design and Experimental Validation of a Metamaterial-Based Sensor for Microwave Imaging in Breast, Lung, and Brain Cancer Detection
PublikacjaThis study proposes an innovative geometry of a microstrip sensor for high-resolution microwave imaging (MWI). The main intended application of the sensor is early detection of breast, lung, and brain cancer. The proposed design consists of a microstrip patch antenna fed by a coplanar waveguide with a metamaterial layer-based lens implemented on the back side, and an artificial magnetic conductor (AMC) realized on as a separate...
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Ultrafast degradation of brilliant cresyl blue under hydrodynamic cavitation based advanced oxidation processes (AOPs)
PublikacjaThe study highlights effectiveness of hydrodynamic cavitation (HC) in the degradation of effluents polluted by Brilliant Cresyl Blue (BCB) dye. Optimal process parameters were cavitation number 0.27, inlet pressure 1.70 bar, temperature 20 °C and pH 7. The efficiency of HC was investigated in combination with other advanced oxidation processes, including the addition of external oxidants (hydrogen peroxide, ozone, and sodium persulfate)...
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Wybrane aspekty zużycia ostrzy skrawających wykonanych z PcBN
PublikacjaObróbka materiałów twardych związana jest z obróbką materiałów o twardości powyżej 50 HRC. Obróbka ta może być obróbką wykończeniową ze względu namożliwość osiągnięcia wysokiej jakości i dokładności przedmiotów obrabianych po tejoperacji. Obróbka materiałów w stanie twardym lub utwardzonym związana jest z zastosowaniem specjalnych narzędzi, które są wykonane z nowych materiałów, takich jak PcBN. W artykule zaprezentowano wybrane...
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Chapter 32. Method to predict the critical micelle concentration of ionic liquids from the molecular volume
PublikacjaAmfifilowość wielu imidazoliowych cieczy jonowych powoduje, że zjawiska powierzchniowe mają znaczny wpływ na właściwości układów zawierających te związki. Dotychczas przeprowadzono wiele badań w celu wyznaczenia krytycznego stężenia micelizacji cieczy jonowych o różnej budowie. Ze względu na możliwość modyfikacji budowy kationu i wyboru anionu oraz ich kombinacji, podjęto próbę opracowania empirycznego równania pozwalajacego na...
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Kinetics and mechanism of the chemically modified starches during gelatinization.
PublikacjaNative starch was silicated by means of microwave-assisted reactions with varying doses of reagents. Resulting products were characterized by DSC and HMR-relaxation methods. It was observed that the hydratation of the silicated starches during gelatinization decreases when compared to that of the native potato starch. The amount of unfrozen water for ungelitinizated silicated starches are less than in control samples. At the same...
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Synthesis and structure determination of 2,3-diaryl-9,9-dioxo-1H-9-thia-1,4,4a,7,10-pentaazaphenanthrene-2-ols
Publikacja3-Amino-1,1-dioxopyrido[4,3-e]-1,4,2-dithiazine has been synthesized and applied to the synthesis of 3-amino-2-(4-thioxo-1,4-dihydropyridin-3-ylsulfonyl)guanidine. The reaction of the aminoguanidine with the appropriate 1,2-diarylethane-1,2-diones afforded 2,3-diaryl-9,9-dioxo-1H-9-thia-1,4,4a,7,10-pentaazaphenanthrene-2-ol derivatives. The structure of these compounds, which represent a novel heterocyclic ring system, was confirmed...
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ASIC Design Example of Complex SoC with FPGA Prototyping
PublikacjaThe paper presents an example of the System on a Chip design, where the FPGA prototyping has been used. Two FPGA prototypes have been realized. The first FPGA prototype uses AVNET board containing Xilinx Virtex4 device accompanied by custom board with required devices. The second FPGA prototype has been built using the custom PCB with Xilinx Virtex-4 XC4VLX60 FPGA accompanied by all needed external components. The final system...
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ASIC Design Example of Complex SoC with FPGA Prototyping
PublikacjaThe paper presents an example of the System on a Chip design, where the FPGA prototyping has been used. Two FPGA prototypes have been realized. The first FPGA prototype uses AVNET board containing Xilinx Virtex4 device accompanied by custom board with required devices. The second FPGA prototype has been built using the custom PCB with Xilinx Virtex-4 XC4VLX60 FPGA accompanied by all needed external components. The final system...
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Cyfrowy akcelerator wybranych modułów standardu kompresji wideo H.264
PublikacjaW artykule przedstawiono konfigurowalny cyfrowy akcelerator estymacji ruchu przeznaczony dla enkodera wideo standardu H.264. Akcelerator został zaimplementowany w technologii FPGA oraz w układzie ASIC w technologii UMC 90 nm. Obie implementacje zostały zweryfikowane, a szczegółowe wyniki pomiarów akceleratora ASIC zostały porównane z innymi dostępnymi w literaturze propozycjami. System został zoptymalizowany do współpracy z oprogramowaniem...