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The extended version of restriction analysis approach for the examination of the ability of low-molecular-weight compounds to modify DNA in a cell-free system
PublikacjaOne of the primary requirements in toxicology is the assessment of ability of chemicals to induce DNA covalent modification. There are several well-established methods used for this purpose such as 32P-Postlabeling or HPLC-MS. However, all of these approaches have difficult to overcome limitations, which prevents their use in genotoxin screening. Here, we describe the simple protocol exploiting specificity of restriction enzymes...
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<title>Cavity simulator and controller for VUV free electron laser SIMCON 2.1, part I: algorithms and SIMCON system</title>
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Selective Photocatalytic Oxidation of 4-Methoxybenzyl Alcohol to p-Anisaldehyde in Organic-Free Water in a Continuous Annular Fixed Bed Reactor
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Nano Tin/Tin Oxide Attached onto Graphene Oxide Skeleton as a Fluorine Free Anode Material for Lithium-Ion Batteries
PublikacjaHerein, we show a composite formation method of tin/tin oxide nanoparticles with graphene oxide and CMC based on laser ablation technique as an electrode material for energy storage devices. The material exhibited a three-dimensional conducting graphene oxide network decorated with tin or tin oxide nanoparticles. The structure, homogeneous distribution of nanoparticles, and direct contact between inorganic and organic parts were...
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Fixed final time and free final state optimal control problem for fractional dynamic systems – linear quadratic discrete-time case
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Blood free Radicals Concentration Determined by Electron Paramagnetic Resonance Spectroscopy and Delayed Cerebral Ischemia Occurrence in Patients with Aneurysmal Subarachnoid Hemorrhage
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<title>Cavity digital control testing system by Simulink step operation method for TESLA linear accelerator and free electron laser</title>
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Boron-doped carbon nanowalls for fast and direct detection of cytochrome C and ricin by matrix-free laser desorption/ionization mass spectrometry
PublikacjaDetecting proteins via surface assisted laser desorption/ionization mass spectrometry (SALDI-MS) method is still highly challenging, and only few examples of nanomaterials have been demonstrated to perform such detection so far. In this study, carbon nanowalls (CNWs), vertically aligned graphene sheet-based materials, presenting specific morphology, dimensions, and boron doping levels have shown improved performances for both qualitative...
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Designing efficient A-D-A1-D-A type fullerene free acceptor molecules with enhanced power conversion efficiency for solar cell applications
PublikacjaThe achievement of highly efficient power conversion efficiency (PCE) is a big concern for non-fullerene organic solar cells (NF-OSCs) because PCE can depend on numerous variables. Here, new five novel acceptor molecules without fullerenes were developed and investigated using DFT (density functional theory) and TD-DFT (time dependent-density functional theory). Compared to the recently synthesized molecule (PZ-dIDTC6), the developed...
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Structure and properties comparison of poly(ether-urethane)s based on nonpetrochemical and petrochemical polyols obtained by solvent free two-step method
PublikacjaThe application of thermoplastic polyurethanes (TPU) is becoming more and more extensive, and the decreasing of used petrochemical monomers and reduction of energy for the polymerization and processing processes is getting increasingly important. In this paper, we confirmed the positive influence of high bio-based monomers contents (by replacing petrochemical polyol and glycol by bio-based counterparts) on processing and properties...
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Author Correction: Decreased integrity of exercise-induced plasma cell free nuclear DNA – negative association with the increased oxidants production by circulating phagocytes
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Tuning Cu-Content La1−xSrxNi1−yCuyO3−δ with Strontium Doping as Cobalt-Free Cathode Materials for High-Performance Anode-Supported IT-SOFCs
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Branched Poly(l-lysine)-Derived Nitrogen-Containing Porous Carbon Flake as the Metal-Free Electrocatalyst toward Efficient Oxygen Reduction Reaction
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Rozmieszczenie wybranych metali ciężkich w ekosystemie oczyszczalni hydrofitowej w Bielkowie.Distribution of heavy metals in ecosystem of free water surface system in Bielkowo.
PublikacjaIn the article the results of investigations of biomass crop and heavy metal contents in bottom sediments and in three species of plants (Glyceria maxima, Typha latifolia)inhabiting a wetland system in Bielkowo, Poland are presented. The measurements were carried out three times during the vegetation season (May, July and October). The contents of metals in different fragments of plants (leaves, stalks and roots) was analyzed....
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The interaction between cuticle free fatty acids (FFAs) of the cockroaches Blattella germanica and Blatta orientalis and hydrolases produced by the entomopathogenic fungus Conidiobolus coronatus
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A label-free graphene-based impedimetric biosensor for real-time tracing of the cytokine storm in blood serum; suitable for screening COVID-19 patients
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Discriminating macromolecular interactions based on an impedimetric fingerprint supported by multivariate data analysis for rapid and label-free Escherichia coli recognition in human urine
PublikacjaThis manuscript presents a novel approach to address the challenges of electrode fouling and highly complex electrode nanoarchitecture, which are primary concerns for biosensors operating in real environments. The proposed approach utilizes multiparametric impedance discriminant analysis (MIDA) to obtain a fingerprint of the macromolecular interactions on flat glassy carbon surfaces, achieved through self-organized, drop-cast,...
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A critical review on biochar-assisted free radicals mediated redox reactions influencing transformation of potentially toxic metals: Occurrence, formation, and environmental applications
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Assessing the influence of sewage sludge and derived-biochar in immobilization and transformation of heavy metals in polluted soil: Impact on intracellular free radical formation in maize
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<title>Cavity simulator and controller for VUV free electron laser SIMCON 2.1, part III: I/O ports and measurement results</title>
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Debonding Size Estimation in Reinforced Concrete Beams Using Guided Wave-Based Method
PublikacjaThe following paper presents the results of the theoretical and experimental analysis of the influence of debonding size on guided wave propagation in reinforced concrete beams. The main aim of the paper is a development of a novel, baseline-free method for determining the total area of debonding between steel rebar embedded in a concrete cover on the basis of the average wave velocity or the time of flight. The correctness of...
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Versatile method employing basic techniques of genetic engineering to study the ability of low molecular weight compounds to bind covalently with DNA in cell free systems.
PublikacjaMechanizm działania większości leków przeciwnowotworowych oraz związków rakotwórczych polega na ich kowalencyjnym wiązaniu z DNA. Poziom tego wiązania jest zazwyczaj bardzo niski, co powoduje, że potrzebne są niezwykle czułe metody aby kowalencyjna modyfikacja mogła być w ogóle wykryta. My podjęliśmy próbę zastosowania w tym celu prostych, szybkich i sprawdzonych technik inżynierii genetycznej: metody PCR do uzyskania fragmentu...
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Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract
PublikacjaNumerous studies have shown, rather disappointingly, that isolated bioactive phytochemicals are not as biologically effective as natural plant products. Such a discrepancy may be explained by the concept of food synergy, which was verified in this research for cocoa extract versus its major components with regard to cancer chemoprevention. The evaluation embraced the relationship between redox properties evaluated in cell-free systems...
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Antibody reactivity in patients with IgE-mediated wheat allergy to various subunits and fractions of gluten and non-gluten proteins from ω-gliadin-free wheat genotypes
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Zastosowanie algorytmu MSA (Multiple Similar Areas) do wyznaczania map głębi w wielowidokowych systemach widzenia komputerowego
PublikacjaArtykuł podejmuje temat pozyskiwania map głębi (ang. depth map) na podstawie zdjęć z wielu kamer w wyniku widzenia stereoskopowego. Mapa głębi zawierająca odległości od obiektów będących w zasięgu widzenia kamer pozyskana może zostać na podstawie zdjęć z co najmniej dwóch kamer pełniących funkcję kamery stereoskopowej. W mapach głębi pozyskanych w ten sposób występują jednak błędy. Artykuł dotyczy metod redukcji błędów dzięki zwiększeniu...
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Interference-Free Determination of Trace Copper in Freshly Ripened Honeys by Flame Atomic Absorption Spectrometry Following a Preconcentration by Solid-Phase Extraction and a Two-Step Elution Process
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Badania jakości docierania stali hartowanej docierakami aktywizowanymi w sposób swobodny = Study of hardened steel lapping quality where lapping tools are activized in a free way
PublikacjaPrzedstawiono badania jakości docierania stali hartowanej docierakami zbrojonymi w sposób swobodny. Analizowano wpływ podstawowych warunków obróbki na chropowatość powierzchni obrobionej. Wyznaczono zależność parametru Ra od prędkości i czasu docierania oraz nacisku jednostkowego. Analizowano również zanieczyszczenie powierzchni ścierniwem po docieraniu.
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Efficacy of rice husk biochar and compost amendments on the translocation, bioavailability, and heavy metals speciation in contaminated soil: Role of free radical production in maize (Zea mays L.)
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Surface-enhanced Raman scattering (SERS) and tip-enhanced Raman scattering (TERS) in label-free characterization of erythrocyte membranes and extracellular vesicles at the nano-scale and molecular level
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Free Amino Acids and Biogenic Amines Content during Ageing of Dry-cured Pork Loins Inoculated with <i>Lactobacillus casei</i> ŁOCK 0900 Probiotic Strain
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The Caucasian flora: a still-to-be-discovered rich source of antioxidants
PublikacjaCellular redox homeostasis is a state of balance between the formation of Usually Reactive Oxygen and / or Nitrogen Species (ROS/RNS), endogenous antioxidant defence systems, and exogenous dietary antioxidants. The disturbance of redox homeostasis, by the overproduction of endogenous ROS/RNS, may increase the risk of development of so-called civilisation diseases. The solution seems to be either the increased production of endogenous...
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Ceragenin CSA-13 as free molecules and attached to magnetic nanoparticle surfaces induce caspase-dependent apoptosis in human breast cancer cells via disruption of cell oxidative balance
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Qualitative analysis of phospholipids and their oxidised derivatives – used techniques and examples of their applications related to lipidomic research and food analysis
PublikacjaPhospholipids (PLs) are important biomolecules that not only constitute structural building blocks and scaffolds of cell and organelle membranes, but also play a vital role in cell biochemistry and physiology. Moreover, dietary exogenous PLs are characterized by high nutritional value and other beneficial health effects, which are confirmed by numerous epidemiological studies. For this reason, PLs are of high interest in lipidomics...
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The Latest Advances in Wireless Communication in Aviation, Wind Turbines and Bridges
PublikacjaPresent-day technologies used in SHM (Structural Health Monitoring) systems in many implementations are based on wireless sensor networks (WSN). In the context of the continuous development of these systems, the costs of the elements that form the monitoring system are decreasing. In this situation, the challenge is to select the optimal number of sensors and the network architecture, depending on the wireless system’s other parameters...
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Telomere uncapping by common oxidative guanine lesions: Insights from atomistic models
PublikacjaOxidative damage to DNA is widely known to contribute to aging and disease. This relationship has been extensively studied for telomeres – structures that cap chromosome ends – due to their role in cell proliferation and senescence, and exceptional susceptibility to oxidation. Indeed, the repetitive telomeric DNA sequence contains the 5′-GGG-3′ motif that has the lowest ionization potential of all trinucleotides. Accordingly, experiments...
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Stereo image visualization for a VISROBOT system
PublikacjaThe article describes a novel approach to robotic vision in mobile robot systems. The system implements a Visrobot system which implements a generic idea of using mobile robots for exploring an indoor environment. The task of such a robot is to visualize a stereo image properly for an operator. The system uses different stereo baseline values. Variable baseline can result in increasing depth resolution for distant objects. We assume...
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Theoretical and experimental analysis of guided wave propagation in plate-like structures with sinusoidal thickness variations
PublikacjaGuided waves have attracted significant attention for non-destructive testing (NDT) and structural health monitoring (SHM) due to their ability to travel relatively long distances without significant energy loss combined with their sensitivity to even small defects. Therefore, they are commonly used in damage detection and localization applications. The main idea of incorporating guided waves in NDT and SHM is based on processing...
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Analiza ruchu drogowego z wykorzystaniem analizy akustycznej
PublikacjaTematyka pracy porusza zagadnienia dotyczące pozyskiwania informacji o ruchu drogowym z wykorzystaniem monitoringu akustycznego. Przybliżono podstawowe techniki nadzoru nad ruchem drogowym. Przedstawiono założenia akustycznego detektora ruchu i zbadano jego skuteczność na trzech płaszczyznach działania – zliczania pojazdów, klasyfikacji rodzajowej i klasyfikacji warunków pogodowych panujących na nawierzchni
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BADANIA KOMPOZYTOWEGO MOSTU DLA PIESZYCH
PublikacjaThe paper presents in situ load testing of the innovative footbridge of FOBRIDGE grant mounted at Gdansk University of Technology. The span of composite sandwich structure (GFRP skins, PET core) and the scope of static and dynamic tests are presented. The selected, representative results of in situ measurements and data obtained from the SHM monitoring system are discussed. Besides the standard testing procedures on the full scale...
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Stiffness change of abdominal prosthesis Optomesh™ under cyclic loading
PublikacjaAbdominal prostheses are used in humans for abdominal wall strengthening or reconstruction. From the mechanical point of view they are membranes, so their basic mechanical property is tensile stiffness. This property is experimentally identified for a selected implant, OptomeshTM. The obtained results are described herein. Uni-axial simple and cyclic tension tests are a basis for the analysis. Both kinds of tests allowed to distinguish...
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Dynamic Response of the Suspended on a Single Cable Footbridge
PublikacjaThe article presents numerical simulations, dynamic in situ load tests and a structural health monitoring (SHM) system installed in a suspended on a single cable footbridge. Numerical simulations performed prior to construction indicated the possibility of structural dynamics problems, finally confirmed in the course of dynamic test loading. In the dynamic load course the bridge deck developed vibrations displaying accelerations...
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Special Issue: “Non-Destructive Testing of Structures”
PublikacjaThe Special Issue “Non-Destructive Testing of Structures” has been proposed to present recent developments in the field of diagnostics of structural materials and components in civil and mechanical engineering. The papers highlighted in this editorial concern various aspects of non-invasive diagnostics, including such topics as condition assessments of civil and mechanical structures and connections of structural elements, the...
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Structural Health Monitoring System for Suspension Footbridge
PublikacjaThe article presents the structural health monitoring (SHM) system installed in a footbridge in Radom. The main reason for the need for fitting of such a system was failed acceptance tests of the bridge. While under a dynamic load consisting of a group of 12 pedestrians jumping on the bridge, the bridge developed vibration during which acceleration of the deck of up to 4.5 m/s2 was recorded. Such behavior of the footbridge causes...
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Structural Health Monitoring of Composite Shell Footbridge for Its Design Validation
PublikacjaThe paper presents the structural health monitoring system of the composite bridge for pedestrians and cyclists. The footbridge was designed and manufactured within the FOBRIDGE project. It is the first bridge in the world, which is classified as a consistent single element structural girder, made of sandwich shells, entirely produced in a single cycle. The developed structural health monitoring system, supported design process,...
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Identification of defected sensors in an array of amperometric gas sensors
PublikacjaPurpose Amperometric gas sensors are commonly used in air quality monitoring in long-term measurements. Baseline shift of sensor responses and power failure may occur over time, which is an obstacle for reliable operation of the entire system. The purpose of this study is to check the possibility of using PCA method to detect defected samples, identify faulty sensor and correct the responses of the sensor identified as faulty. Design/methodology/approach In...
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An adaptive-noise Augmented Kalman Filter approach for input-state estimation in structural dynamics
PublikacjaThe establishment of a Digital Twin of an operating engineered system can increase the potency of Structural Health Monitoring (SHM) tools, which are then bestowed with enhanced predictive capabilities. This is particularly relevant for wind energy infrastructures, where the definition of remaining useful life is a main driver for assessing the efficacy of these systems. In order to ensure a proper representation of the physical...
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Results of the application of tropospheric corrections from different troposphere models for precise GPS rapid static positioning
PublikacjaIn many surveying applications, determination of accurate heights is of significant interest. The delay caused by the neutral atmosphere is one of the main factors limiting the accuracy of GPS positioning and affecting mainly the height coordinate component rather than horizontal ones. Estimation of the zenith total delay is a commonly used technique for accounting for the tropospheric delay in static positioning. However, in the...
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Sport-related concussion alters cerebral hemodynamic activity during controlled respiration
PublikacjaThis study investigated cerebral hemodynamic activity following sport-related concussion. Prefrontal cortex oxygenation was assessed by near-infrared spectroscopy (NIRS) during a controlled breathing protocol. Wavelet transformation of the NIRS signals showed significant decreases in HbO2 and HbD amplitude at the respiratory frequency interval (0.10–0.6 HZ) from preseason baseline to acute concussion. These results suggest a decreased...
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Short and Long Term Measurements in Assessment of FRP Composite Footbridge Behavior
PublikacjaThe paper presents application of different sensors for the purpose of short and long term measurements, as well as a structural health monitoring (SHM) system to assess the behavior of a novel fiber reinforced plastics (FRP) composite footbridge. The aim is to present a thorough and concise description of these sensors networks and results gathered with their aid during in situ measurement of strains, displacements, and vibrations,...
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The Effect of Oxygenated Diesel-N-Butanol Fuel Blends on Combustion, Performance, and Exhaust Emissions of a Turbocharged CRDI Diesel Engine
PublikacjaThe article deals with the effects made by using various n-butanol-diesel fuel blends on the combustion history, engine performance and exhaust emissions of a turbocharged four-stroke, four-cylinder, CRDI 1154HP (85 kW) diesel engine. At first, load characteristics were taken when running an engine with normal diesel fuel (DF) to have ‘baseline’ parameters at the two ranges of speed of 1800 and 2500 rpm. Four a fossil diesel (class...