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Wyniki wyszukiwania dla: ANALYTICAL METHODS
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Identification and properties of the psychrophilic bacterium Desulfolatea psychrophila single-stranded DNA binding proteins
PublikacjaTo study the biochemical properties of SSB from Desulfolatea psychrophila (DpsSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 426 nucleotides encoding SSB protein of 142 amino acids with a calculated molecular mass of 15.6 kDa and it is the smallest known bacterial SSB protein indentified to date. The amino-acid sequence...
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Comprehensive Comparison of a Few Variants of Cluster Analysis as Data Mining Tool in Supporting Environmental Management
PublikacjaA few variants of hierarchical cluster analysis (CA) as tool of assessment of multidimensional similarity in environmental dataset are compared. The dataset consisted of analytical results of determination of metals (Na, K, Ca, Sc, Fe, Co, Zn, As, Br, Rb, Mo, Sb, Cs, Ba, La, Ce, Sm, Hf and Th) in ambient air dried and kept alive, by the means of hydroponics, moss baskets collected in 12 locations on the area of Tricity (Poland)....
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Relatively high-Seebeck thermoelectric cells containing ionic liquids supplemented by cobalt redox couple
PublikacjaMeanwhile no general and reliable equation determining the Seebeck coefficient (S e ) and involving electro-chemical reaction effects was derived for solutions. We reported the database of 15,000 ionic liquids supplemented by three different redox couple systems: 0.01 mol/l Co 3+/2+ (bpy) 3 , 0.01 mol/l I 3-/3I- and 0.2 mol/l 3- /3I- , and the corresponding estimated Seebeck coefficients. We also reported methods for estimating...
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Experimental investigation of joint with positive eccentricity in CFS truss
PublikacjaDue to technological restrictions, in the case of trusses made of CFS open cross-sections, positive eccentricities in the truss joints have become very frequent. Therefore studies concerning the load-bearing capacity of truss joints with positive eccentricity were undertaken. It was assumed that the resistance of cold-formed steel (CFS) open cross-section truss joints located on compression chords and with positive eccentricity...
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Fast Design Closure of Compact Microwave Components by Means of Feature-Based Metamodels
PublikacjaPrecise tuning of geometry parameters is an important consideration in the design of modern microwave passive components. It is mandatory due to limitations of theoretical design methods unable to quantify certain phenomena that are important for the operation and performance of the devices (e.g., strong cross-coupling effects in miniaturized layouts). Consequently, the initial designs obtained using analytical or equivalent network...
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Application of the Msplitmethod for filtering airborne laser scanning data-sets to estimate digital terrain models
PublikacjaALS point cloud filtering involves the separation of observations representing the physical terrain surface from those representing terrain details. A digital terrain model (DTM) is created from a subset of points representing the ground surface. The accuracy of the generated DTM is influenced by several factors, including the survey method used, the accuracy of the source data, the applied DTM generation algorithm, and the survey...
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Numerical Issues and Approximated Models for the Diagnosis of Transmission Pipelines
PublikacjaThe chapter concerns numerical issues encountered when the pipeline flow process is modeled as a discrete-time state-space model. In particular, issues related to computational complexity and computability are discussed, i.e., simulation feasibility which is connected to the notions of singularity and stability of the model. These properties are critical if a diagnostic system is based on a discrete mathematical model of the flow...
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Application of Msplit method for filtering airborne laser scanning data sets to estimate digital terrain models
PublikacjaALS point cloud filtering involves the separation of observations representing the physical terrain surface from those representing terrain details. A digital terrain model (DTM) is created from a subset of points representing the ground surface. The accuracy of the generated DTM is influenced by several factors, including the survey method used, the accuracy of the source data, the applied DTM generation algorithm, and the survey...
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Synteza układu sterowania statkiem morskim dynamicznie pozycjonowanym w warunkach niepewności
PublikacjaNiniejsza monografia obejmuje zagadnienia związane z syntezą układu dynamicznego pozycjonowania statku w środowisku morskim z zastosowaniem wybranych nieliniowych metod sterowania. W ramach pracy autorka rozważała struktury sterowania z zastosowaniem wektorowej adaptacyjnej metody backstep oraz metod jej pokrewnych, takich jak regulatory MSS (ang. multiple surface sliding), DSC (ang. dynamic surface control), NB (ang. neural backstepping)....
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The use of fast molecular descriptors and artificial neural networks approach in organochlorine compounds electron ionization mass spectra classification
PublikacjaDeveloping of theoretical tools can be very helpful for supporting new pollutant detection. Nowadays, a combination of mass spectrometry and chromatographic techniques are the most basic environmental monitoring methods. In this paper, two organochlorine compound mass spectra classification systems were proposed. The classification models were developed within the framework of artificial neural networks (ANNs) and fast 1D and...
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Coupled nonlinear Schrödinger equations in optic fibers theory
PublikacjaIn this paper a detailed derivation and numerical solutions of CoupledNonlinear Schr¨odinger Equations for pulses of polarized electromagnetic wavesin cylindrical fibers has been reviewed. Our recent work has been compared withsome previous ones and the advantage of our new approach over other methods hasbeen assessed. The novelty of our approach lies is an attempt to proceed withoutloss of information within the frame of basic...
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Identification and characterization of the Indian Yellow dyestuff and its degradation products in historical oil paint tube by liquid chromatography mass spectrometry
PublikacjaAn analytical protocol for identification of dyes using reversed phase liquid chromatography-mass spectrometry with atmospheric pressure electrospray ionization (LC-ESI/MS) is presented. The developed method has been successfully applied in identification of euxanthic acid and euxanthone, the main components of the Indian Yellow dye in a historical oil paint tube produced by Richard Ain`es, a recognizable French company supplying...
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Human serum interactions with phenolic and aroma substances of Kaffir(Citrus hystrix) and Key lime (Citrus aurantifolia) juices
PublikacjaTo understand the therapeutic application of polyphenols extracted from Kaffir (PolKaf) and Key (PolKey) limesdifferent analytical methods were applied. Based on quantitative analysis by two dimensional gas chromato-graphy (GC×GC) and time of flight mass spectrometry (TOFMS) it can be observed that the biggest differencesin the contents of selected terpenes of Kaffir and Key limes occur in chemical compounds such as limonene, citraland...
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A highly thermostable, homodimeric single-stranded DNA-binding protein from Deinococcus radiopugnans
PublikacjaWe report the identification and characterization of the single-stranded DNA-binding protein (SSB) from the mesophile and highly radiation-resistant Deinococcus radiopugnans (DrpSSB). PCR-derived DNA fragment containing the complete structural gene for DrpSSB protein was cloned and expressed in Escherichia coli. The gene consisting of an open reading frame of 900 nucleotides encodes a protein of 300 amino acids with a calculated...
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A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants
PublikacjaThe World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and...
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Effect of wet Hydrogen Sulfide on Carbon Steels Degradation in Refinery Based on Case Study
PublikacjaMaterial degradation caused by wet hydrogen sulfide (Wet H2S) is one of the major issues in refineries. Carbon steel is the main construction material used in refineries and is subjected not only to thickness loss but the hydrogen is the major concern. H2S leads to high atomic hydrogen penetration in steel which causes degradation in the form of blistering and/or cracking (HIC/SOHIC) and stress cracking in the area of welds and...
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Analysis of friction ridge evidence for trace amounts of paracetamol in various pharmaceutical industries by Raman spectroscopy
PublikacjaThe detection of potentially harmful substances presents a multifaceted challenge. On one hand, it can directly save lives, on the other, it can significantly aid and enhance police work, thereby increasing the effectiveness of investigations. The research conducted in this study primarily aims to identify paracetamol in fingerprints, considering situations involving direct contact of a person with paracetamol either chronically...
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Analityczna weryfikacja dokładności komercyjnych programów komputerowych wspomagających projektowanie układów geometrycznych toru
PublikacjaW pracy podjęto próbę wyjaśnienia kwestii uzyskiwanej dokładności w projektowaniu układów geometrycznych toru z wykorzystaniem komercyjnych programów komputerowych typu CAD. Użytkownikowi najczęściej nie są znane zasady działania tych programów, w tym również wykorzystywane algorytmy obliczeniowe. Opisano w sposób ogólny funkcjonowanie dwóch wiodących programów. Przedstawiono własną, analityczną metodę projektowania układów torowych,...
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Żółte barwniki organiczne w XIX-wiecznych farbach Jana Matejki - identyfikacja substancji barwiących, nośników, oraz wypełniaczy
PublikacjaNaturalne barwniki organiczne można znaleźć w wielu obiektach dziedzictwa kulturowego. Identyfikacja substancji barwiących obecnych w farbach historycznych dostarcza istotnych informacji dla wielu specjalistów zaangażowanych w naukę o sztuce. Identyfikacja składu farb pozwala na zastosowanie odpowiednich procedur dotyczących renowacji i konserwacji historycznych dzieł sztuki. Informacje te pozwalają na ich renowację zgodnie z decyzjami...
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Influence of pH and low-molecular weight organic compounds in solution on selected spectroscopic and analytical parameters of flowing liquid anode atmospheric pressure glow discharge (FLA-APGD) for the optical emission spectrometric (OES) determination of Ag, Cd, and Pb
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Modeling organic nitrogen conversions in activated sludge bioreactors
PublikacjaFor biological nutrient removal (BNR) systems designed to maximize nitrogen removal, the effluent total nitrogen (TN) concentration may range from 2.0 to 4.0 g N/m3 with about 25-50% in the form of organic nitrogen (ON). In this study, current approaches to modeling organic N conversions (separate processes vs. constant contents of organic fractions) were compared. A new conceptual model of ON conversions was developed and combined...
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Modeling of medium flow processes in transportation pipelines - the synthesis of their state-space models and the analysis of the mathematical properties of the models for leak detection purposes
PublikacjaThe dissertation concerns the issue of modeling the pipeline flow process under incompressible and isothermal conditions, with a target application to the leak detection and isolation systems. First, an introduction to the model-based process diagnostics is provided, where its basic terminology, tools, and methods are described. In the following chapter, a review of the state of the art in the field of leak detection and isolation...
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Buckling analysis of piezo-magnetoelectric nanoplates in hygrothermal environment based on a novel one variable plate theory combining with higher-order nonlocal strain gradient theory
PublikacjaIn the present investigation, a new first-order shear deformation theory (OVFSDT) on the basis of the in-plane stability of the piezo-magnetoelectric composite nanoplate (PMEN) has been developed, and its precision has been evaluated. The OVFSDT has many advantages compared to the conventional first-order shear deformation theory (FSDT) such as needless of shear correction factors, containing less number of unknowns than the existing...
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Synthesis, structural characterization and reactivity of new trisubstituted N1-acylamidrazones: solid state and solution studies
PublikacjaA series of new linear trisubstituted N1-acylamidrazones have been investigated using a variety of analytical techniques and theoretical calculations to check the influence of the type of N1-acyl substituent on the resonance forms and conformational behavior in the solid state and in solution. The 1D- and 2D-NMR experiments, supported by computational studies, revealed that in solution all amidrazones exhibit conformational syn/anti...
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On time-dependent nonlinear dynamic response of micro-elastic solids
PublikacjaA new approach to the mechanical response of micro-mechanic problems is presented using the modified couple stress theory. This model captured micro-turns due to micro-particles' rotations which could be essential for microstructural materials and/or at small scales. In a micro media based on the small rotations, sub-particles can also turn except the whole domain rotation. However, this framework is competent for a static medium....
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Machine learning-based seismic response and performance assessment of reinforced concrete buildings
PublikacjaComplexity and unpredictability nature of earthquakes makes them unique external loads that there is no unique formula used for the prediction of seismic responses. Hence, this research aims to implement the most well-known Machine Learning (ML) methods in Python software to propose a prediction model for seismic response and performance assessment of Reinforced Concrete Moment-Resisting Frames (RC MRFs). To prepare 92,400 data...
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Design and material selection for a patient transport device in field hospitals
PublikacjaACKGROUND: A major cause of patient and hospital worker injuries occurs transferring patients between two planes. The main aim of this paper was to propose a design of patient’s lift and transfer apparatus for use in field hospitals. The assumption was to design lightweight, durable and ergonomic device using innovative material. The authors concentrated on partial elimination of manual lifting in order to device could work both...
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Techniki zwiększania efektywności metody elementów skończonych poprzez redukcję dziedziny obliczeniowej z wykorzystaniem własności geometrii struktur
PublikacjaWspółczesna elektronika ze względu na swój szybki rozwój wymaga od nas efektywnego modelowania zjawisk polowych. Celem rozprawy jest zwiększanie efektywności metody elementów skończonych poprzez redukcję dziedziny obliczeniowej z wykorzystaniem własności geometrii struktur oraz jej hybrydyzację z użyciem technik analitycznych. Rozprawa zawiera przegląd stanu wiedzy na temat dostępnych obecnie technik modelowania jak również opis...
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NUTRITIONAL AND PHARMACEUTICAL APPLICATIONS OF BIOACTIVE COMPOUNDS IN TROPICAL FRUITS
PublikacjaDuring the past two decades our international scientific group investigated in vitro the physicochemical and nutritional characteristics of some cultivars of durian at various stages of ripening, mangosteen and new cultivars of snake fruit in comparison with widely used avocado, kiwifruit and mango, and in vivo their influence on laboratory animals. The main objective of the present study was to screen and compare the properties...
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Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, and Photobacterium profundum
PublikacjaBackground: Single-stranded DNA-binding proteins (SSBs) play essential roles in DNA replication, recombination and repair in Bacteria, Archaea and Eukarya. In recent years, there has been an increasing interest in SSBs, since they find numerous applications in diverse molecular biology and analytical methods. Results: We report the characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea...
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Characterization of a single-stranded DNA-binding-like Protein from Nanoarchaeum equitans - a nucleic acid binding protein with broad substrate specificity
PublikacjaBackground SSB (single-stranded DNA-binding) proteins play an essential role in all living cells and viruses, as they are involved in processes connected with ssDNA metabolism. There has recently been an increasing interest in SSBs, since they can be applied in molecular biology techniques and analytical methods. Nanoarchaeum equitans, the only known representative of Archaea phylum Nanoarchaeota, is a hyperthermophilic, nanosized,...
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Algorithmic Human Resources Management - Perspectives and Challenges
PublikacjaTheoretical background: Technology – most notably processes of digitalisation, the use of artificial intelligence, machine learning, big data and prevalence of remote work due to pandemic – changes the way organizations manage human resources. One of the increasing trends is the use of so-called “algorithmic management”. It is notably different than previous e-HRM or HRIS (human resources information systems) applications, as it...
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Physics-Based Coarse-Grained Modeling in Bio- and Nanochemistry
PublikacjaCoarse-grained approaches, in which groups of atoms are represented by single interaction sites, are very important in biological and materials sciences because they enable us to cover the size- and time-scales by several orders of magnitude larger than those available all-atom simulations, while largely keeping the details of the systems studied. The coarse-grained approaches differ by the scheme of reduction and by the origin...
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Low-Cost Quasi-Global Optimization of Expensive Electromagnetic Simulation Models by Inverse Surrogates and Response Features
PublikacjaConceptual design of contemporary high-frequency structures is typically followed by a careful tuning of their parameters, predominantly the geometry ones. The process aims at improving the relevant performance figures, and may be quite expensive. The reason is that conventional design methods, e.g., based on analytical or equivalent network models, often only yield rough initial designs. This is especially the case for miniaturized...
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Assessment of the factors influencing on the formation of energy-oriented modes of electric power consumption by water-drainage installations of the mines
PublikacjaPurpose. Performing the analysis to determine energy-efficient modes and assess the characteristics of the main indicators of electric power consumption by mine water-drainage installations based on the developed research mathematical model. Methods. To achieve the purpose set, a methodology is used to develop the multiple multifactor correlation-regression modeling with respect to the modes of electric power consumption by electrical...
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Funkcjonowanie zespołów napędu hydraulicznego maszyn w niskich temperaturach otoczenia
PublikacjaW pracy przedstawiono cztery warianty klasyfikacji rozruchów oziębionego napędu hydraulicznego. Pierwsza sytuacja dotyczy zagadnienia, w którym wszystkie zespoły układu napędowego wraz z olejem mają taką samą niską temperaturę podczas rozruchu. Pozostałe warianty odnoszą się do rozruchu w warunkach szoku termicznego wywołanych zasilaniem ciepłym czynnikiem roboczym oziębionych zespołów hydraulicznych. Rozruch zespołów...
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Stateczność i niezawodność pełnomorskich platform wiertniczych
PublikacjaW pracy przedstawiono wieloletnie doświadczenia Autora w zakresie wybranych aspektów numerycznej analizy pełnomorskich platform wiertniczych, w szczególności stacjonarnych platform stalowych poddanych działaniu fal wiatrowych i wiatru. W opisie zachowania się platform uwzględniono współoddziaływanie trzech ośrodków: materiału konstrukcji, morskich fal wiatrowych i podłoża gruntowego. Przyjęcie do opisu stochastycznych właściwości...
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Deep eutectic solvent-based shaking-assisted extraction for determination of bioactive compounds from Norway spruce roots
PublikacjaPolyphenolic compounds play an essential role in plant growth, reproduction, and defense mechanisms against pathogens and environmental stresses. Extracting these compounds is the initial step in assessing phytochemical changes, where the choice of extraction method significantly influences the extracted analytes. However, due to environmental factors, analyzing numerous samples is necessary for statistically significant results,...
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RESEARCH OF HYDRAULIC PISTON PUMPS’ START-UP IN LOW AMBIENT TEMPERATURES
PublikacjaStarting hydraulic pumps in low ambient temperatures may cause: decreased hydraulic-mechanical efficiency, larger vibration of hydraulic installation, faster wear and more frequent damage to the elements of pumps, change of material properties of elements, increase of pressure drop. As a result of increase of resistance of cold oil flow in the suction pipe, large underpressure may be experienced, causing incomplete filling of pump...
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Ekspertyza dotycząca bezzasadności zarzutów odnośnie parametrów metrologicznych analizatorów pyłu PM2,5 postawionych firmom EAS Envimet Polska Sp. z o.o. i EAS Envimet Analytical Systems GmbH przez spółkę MLU Sp. z o.o. z siedzibą w Katowicach w postępowaniu odwoławczym o sygn. akt: KIO/UZP 435/09
PublikacjaW ekspertyzie sporządzonej dla EAS Envimet Polska sp. z o.o. z siedzibą w Wieliczce wykazano bezzasadność zarzutów zgłoszonych przez Spółkę MLU w Katowicach w odniesieniu do urządzenia BAM1020 w postępowaniu odwoławczym toczącym się przed Prezesem Urzędu Zamówień Publicznych w związku z zaskarżeniem rozstrzygnięcia przetargu o zamówienie publiczne nr DM/5211-06-02/09/ABA na zakup automatycznego miernika pyłu zawieszonego PM2,5...
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State of the art in sample preparation for human breast milk metabolomics—merits and limitations
PublikacjaHuman breast milk (HBM) contains metabolites that are diverse in chemical structure and concentration. These metabolites range from high-abundance to low-abundance compounds and from polar to nonpolar. This complex nature of HBM makes HBM samples difficult to analyze, especially in global metabolomic studies for the determination and characterization of complete sets of metabolites. In this review, the state of the art in sample...
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Passive sampling and/or extraction techniques in environmental analysis: a review
PublikacjaThe current state-of-the-art of passive sampling and/or extraction methods for long-term monitoring of pollutants in different environmental compartments is discussed in this review. Passive dosimeters that have been successfully used to monitor organic and inorganic contaminants in air, water, sediments, and soil are presented. The application of new approaches to the determination of pollutants at the sampling stage is discussed....
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Opinions and view of entrepreneurs regerding the single european Market-Analytical Model. W: Entrepreneurship and small business development in the21st century. Przedsiębiorczość i rozwój małych i średnich przedsiebiorstww XI wieku. Ed. B. Piasecki. Łódź: Uniw. Łódź**2002 s. 109-117.**2002 s. 109 - 117 Opinie i poglądy przedsiębiorców na temat Jednolitego Rynku Europejskiego - model analityczny.
PublikacjaArtykuł zawiera propozycje modelu czynników kształtujących świadomość pol-skich przedsiębiorców odnośnie szans i zagrożeń w związku z otwarciem gospodarki Polski na Jednolitu Rynek Europejski. Model ten musi być wykorzystany przy projektowaniu badań opinii i poglądów przedsiębiorców.
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Secondary emission influenced fluorescence decay of a homogeneous fluorophore solution
PublikacjaAn analytical expression is found allowing the calculation of the secondary emission influenced fluorescence decay of a homogeneous fluorophore solution. Before starting the calculation one has to know the shape of the primary fluorescence decay of the fluorophore and the value of the parameter kappa denoting the ratio of the secondary to primary steady-state fluorescence intensities. The method elaborated by Budo and Ketskemety...
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Polyethylene glycol-coated solid-phase microextraction fibres for the extraction of polar analytes-A review
PublikacjaThe article discusses the merits and limitations of commercially available solid-phase microextraction (SPME) fibres and compares them with the new type of extraction coatings, in particular those containing polyethylene glycol as sorbent, as well as the methods of the preparation of the latter. It also analyses their possible application for the extraction of a broad spectrum of analytes, with particular emphasis on the sampling...
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Application of gas chromatography to the analysis of spirit-based alcoholic beverages
PublikacjaSpirit-based beverages are alcoholic drinks, which production processes are dependent on the type and origin of raw materials. The composition of this complex matrix is difficult to analyze and scientists commonly choose a gas chromatography techniques for this reason. With a wide selection of extraction methods and detectors it is possible to provide qualitative and quantitative analysis for many chemical compounds with a various...
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Three phase transient model of wet coal pyrolysis
PublikacjaA one-dimensional transient mathematical model was developed to describe the thermal and flow phenomena during coal pyrolysis in a coke oven. The model was solved numerically using partly implicit methods for gas flow and heat transfer problems. It was successfully validated with industrial-scale measurements of temperature change in the middle-plane of the coke oven chamber. The evolution of temperature and pressure, distributions...
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Devices for the production of reference gas mixtures
PublikacjaFor many years there has been growing demand for gaseous reference materials, which is connected with development in many fields of science and technology. As a result, new methodological and instrumental solutions appear that can be used for this purpose. Appropriate quality assurance/quality control (QA/QC) must be used to make sure that measurement data are a reliable source of information. Reference materials are a significant...
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Advances in mass spectrometry for iron speciation in plants
PublikacjaIron is an important nutrient essential for plants and critical for human health. The state-of-the art of methods for iron speciation in cereal grains and plant fluids is critically reviewed. Particular attention is given to the latest developments in the coupling of HPLC with the parallel ICP MS and electrospray ionization (ESI) MS/MS detection, usually QTOF MS or Q-Orbitrap MS, for the identification and quantification of iron...
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On the geometrically nonlinear vibration of a piezo-flexomagnetic nanotube
PublikacjaIn order to describe the behavior of thin elements used in MEMS and NEMS, it is essential to study a nonlinear free vibration of nanotubes under complicated external fields such as magnetic environment. In this regard, the magnetic force applied to the conductive nanotube with piezo-flexomagnetic elastic wall is considered. By the inclusion of Euler-Bernoulli beam and using Hamilton’s principle, the equations governing the system...