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Wyniki wyszukiwania dla: ESCHERICHIA COLI . WASTEWATER TREATMENT PLANT . MARINE OUTFALLS . INTEGRONS . GENE CASSETTES . ANTIBIOTIC RESISTANCE
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Antibiotic resistance and prevalence of class 1 and 2 integrons in Escherichia coli isolated from two wastewater treatment plants, and their receiving waters (Gulf of Gdansk, Baltic Sea, Poland)
PublikacjaIn this study, antimicrobial-resistance patterns were analyzed in Escherichia coli isolates from raw (RW) and treated wastewater (TW) of two wastewater treatment plants (WWTPs), their marine outfalls (MOut), and mouth of the Vistula River (VR). Susceptibility of E. coli was tested against different classes of antibiotics. Isolates resistant to at least one antimicrobial agent were PCR tested for the presence of integrons. Ampicillin-resistant...
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Diversity of fecal coliforms and their antimicrobial resistance patterns in wastewater treatment model plant.
PublikacjaThe occurrence of resistance patterns among wastewater fecal coliforms was determined in the study. Susceptibility of the isolates was tested against 19 antimicrobial agents: aminoglycosides, aztreonam, carbapenems, cephalosporines, b-lactam/b-lactamase inhibitors, penicillines, tetracycline, trimethoprim/sulfamethoxazole, and fluoroquinolones. Additionally the removal of resistant isolates was evaluated in the laboratory-scale...
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Human-associated bacteria and their mobile genetic elements - important minority in wastewater and wastewater impacted ecosystems
PublikacjaSince human-associated bacteria have been recognized as vectors in two - way gene transmission between the pathogenic and environmental populations, health hazard connected with wastewater discharge requires reevaluation. In this paper the particular attention was given to antimicrobial-resistance of Escherichia coli and enterococci isolated from wastewater and wastewater impacted environments. The obtained results showed that...
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Antimicrobial resistance of fecal indicators in muncipal wastewater treatment plant
PublikacjaAntimicrobial resistance of fecal coliforms (n = 153) and enterococci (n = 199) isolates was investigated in municipal wastewater treatment plant (WWTP) based on activated sludge system. The number of fecal indicators (in influent and effluent as well as in the aeration chamber and in return activated sludge mixture) was determined using selective media. Susceptibility of selected strains was tested against 19 (aminoglycosides,...
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The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)
PublikacjaThe aim of this study was to analyze the occurrence of sulfonamide resistance genes (sul1–3) and other genetic elements as antiseptic resistance gene (qacEΔ1) and class 1 and class 2 integrons (int1–2) in the upper layer of substrate and in the effluent of microcosm constructed wetlands (CWs) treating artificial wastewater containing diclofenac and sulfamethoxazole (SMX), which is a sulfonamide antibiotic. The bacteria in the substrate...
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Antibiotic resistance, virulence, and phylogenetic analysis of Escherichia coli strains isolated from free-living birds in human habitats
PublikacjaWild birds can be colonized by bacteria, which are often resistant to antibiotics and have various virulence profiles. The aim of this study was to analyze antibiotic resistance mechanisms and virulence profiles in relation to the phylogenetic group of E. coli strains that were isolated from the GI tract of wildfowl. Out of 241 faecal samples, presence of E. coli resistant to a cephalosporin (ESBL/AmpC) was estimated for 33 isolates...
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Enzymatic degradation and transport of endothiopeptides into Escherichia coli K12 mutant strains
PublikacjaZbadano transport trzech otrzymanych na drodze syntezy ditiopeptydów do komórek Escherichia coli K12. Ponadto zbadano szybkość hydrolizy tych związków. Wszystkie peptydy są transportowane przez permeazy, natomiast są bardziej oporne na hydrolizę enzymatyczną w stosunku do naturalnych peptydów.
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Resistance of Escherichia coli and Enterococcus spp. to selected antimicrobial agents present in municipal
PublikacjaIn this study, the susceptibility to erythromycin (E) and to trimethoprim/sulfamethoxazole (SXT) among isolates of Enterococcus spp. and Escherichia coli was tested, respectively. Both fecal indicators were detected and isolated from raw(RW) and treated wastewater (TW) as well as from samples of activated sludge (AS) collected in a local wastewater treatment plant (WWTP). Biodiversity of bacterial community in AS was also monitored...
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A two-plasmid Escherichia coli system for expression of Dr adhesins
PublikacjaPraca opisuje konstrukcję wydajnego systemu produkcji fimbrii Dr. System składa się z dwóch plazmidów. Plazmid pACYCpBAD-DraC-C-His zawiera pod kontrolą promotora arabinozowego gen draC kodujący białko kanałotwórcze DraC. Plazmid pET-30b-sygDraBE zawiera pod kontrolą promotora T7lac geny draB i draE kodujące odpowiednio białko opiekuńcze DraB oraz adhezynę DraE. Oba plzamidy posiadają różne ori replikacji co pozwala na ich jednoczesne...
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Antimicrobial resistance of Pseudomonas spp. isolated from wastewater and wastewater-impacted marine coastal zone
PublikacjaIn this study, species distribution and antimicrobial susceptibility of cultivated Pseudomonas spp. were studied in influent (INF), effluent (EFF), and marine outfall (MOut) of wastewater treatment plant (WWTP). The susceptibility was tested against 8 antimicrobial classes, active against Pseudomonas spp.: aminoglycosides, carbapenems, broad-spectrum cephalosporins from the 3rd and 4th generation, extended-spectrum penicillins,...
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Molecular typing of clinical isolates of Escherichia coli by a new PCR MP method
PublikacjaTypowanie epidemiologiczne bakterii jest rutynową procedurą stosowaną w badaniach zakażeń szpitalnych i wymaga stosowania odpowiednich metod o wysokim potencjale różnicującym. Przedstawiliśmy możliwości nowej techniki PCR MP w różnicowaniu szczepów E. coli. Rezultaty zostały porównane z wynikami uzyskanymi metodą PFGE.
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Molecular typing of clinical isolates of Escherichia coli by a new PCR MP method
PublikacjaTypowanie epidemiologiczne bakterii jest rutynową procedurą stosowaną w badaniach zakażeń szpitalnych i wymaga stosowania odpowiednich metod o wysokim potencjale różnicującym. Przedstawiliśmy możliwości nowej techniki PCR MP w różnicowaniu szczepów E. coli. Rezultaty zostały porównane z wynikami uzyskanymi metodą PFGE.
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Antibiotic resistance in wastewater, does the context matter? Poland and Portugal as a case study
PublikacjaAntibiotic resistance has been considered a major human health threat that may endanger the success of medicine. Recent studies have unveiled worldwide asymmetries of antibiotic resistance occurrence, being factors as diverse as climate, socioeconomic, or antibiotic use possible drivers of such asymmetric distribution. In Europe, where clinical antibiotic resistance is surveyed for more than 20 years, the European Center for Disease...
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Type II secretory pathway for surface secretion of DraD invasin from the uropathogenic Escherichia coli Dr+ strain
PublikacjaWłaściwości wirulentne uropatogennych szczepów Escherichia coli Dr+ IH11128 związane są z obecnością struktur fimbrialnych typu Dr i białka inwazyny DraD, które może na powierzchni komórki bakteryjnej pełnić rolę fimbrialnej podjednostki szczytowej. Ostatnie badania wykazały, że białko DraD jest eksponowane na powierzchni bakterii w dwóch formach: związanej ze strukturą fimbrialną i jako niezależna od fimbrii otoczka białkowa....
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A freeze-thaw method for desintegration of Escherichia coli cells producing T7 lysozyme used in pBAD expression systems
PublikacjaPlazmid pLysN zawierający gen kodujący lizozym T7 pod kontrolą promotora lac został skonstruowany w celu ułatwienia dezintegracji komórek po ekspresji rekombinantowych białek w systemach ekspresji indukowanych arabinozą. Użyteczność plazmidu została przetestowana w komórkach Escherichia coli TOP10 i E. coli LMG194, niosących plazmid pBADMHADgeSSB, zawierający gen kodujący białko SSB Deinococcus geothermalis pod kontrolą promotora...
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Tetracycline and ampicillin resistance of Escherichia coli strains of surface water origin: the potential for horizontal transfer of resistance genes = Oporność na tetracyklinę i ampicylinę szczepów Escherichia Coli wyizolowanych z wody powierzchniowej : możliwość horyzontalnego przenoszenia genów oporności
PublikacjaThe aim of this preliminary study was to assess the occurrence and molecular diversity of tetracycline and ampicillin resistance genes carried by Escherichia coli present in surface water. Bacterial strains were isolated from two watercourses (the Reda River and the Oliwski Stream) that influence the quality of coastal waters in the Gdańsk Bay (Northern Poland) by direct discharge. The bacterial drug susceptibility, tested against...
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Antibiotic resistance among Escherichia coli urinary isolates and their susceptibility to clove essential oil
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A five-year retrospective study shows increasing rates of antimicrobial drug resistance in Cabo Verde for both Staphylococcus aureus and Escherichia coli
PublikacjaObjectives Data on baseline drug resistance is important in informing future antimicrobial stewardship programs. So far, no data on the antimicrobial drug resistance of clinical isolates was available for the African archipelago of Cabo Verde. Methods We have performed a retrospective analysis over five-years (2013-17) of the antimicrombial drug susceptibility profiles of clinical isolates in the two main hospitals of Cabo Verde....
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Expression of Deinococcus geothermalis Trehalose Synthase Gene in Escherichia coli and Its Enzymatic Properties
PublikacjaA novel trehalose synthase gene from Deinococcus geothermalis (DSMZ 11300) containing 1,692 bp reading-frame encoding 564 amino acids was amplified using PCR. The gene was ligated into pET30Ek/LIC vector and expressed after isopropyl alfa-D-thiogalactopyranoside induction in Escherichia coli BL21(DE3)pLysS. The recombinant trehalose synthase (DgeoTreS) containing a His6 tag at the C-terminus was purified by metal affinity chromatography...
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Identification of Selected Antibiotic Resistance Genes in Two Different Wastewater Treatment Plant Systems in Poland: A Preliminary Study
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Characterization of immobilized Escherichia coli cells transformed by β-galactosidase gene from Pyrococcus woesei
PublikacjaThermostable β-galactosidase from Escherichia coli transformant containing theenzyme gene from Pyrococcus woesei was immobilized on alginate gel.The benefits of using whole bacterial cells not only exclude expensive, laborious proteinisolation and purification but also stabilize enzymes by cytosol components. Increase inproductivity of enzyme can be achieved by cells permeabilization. To increase permeability ofcytoplasmic membrane...
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Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli.
PublikacjaGen kodujący alfa-amylazę z Pyrococcus woesei klonowano do plazmidów pET21d(+)lub pYTB2, którymi transformowano komórki Escherichia coli. Otrzymana rekombinantowa alfa amylaza wykazuje maksymalną aktywność przy pH 5.6 w temperaturze 95 stC i przejawia około 24 % początkowej aktywności nawet po 2 godz. inkubacji w 120 stC. Duża termostabilność otrzymanego preparatu świadczy o jego przydatności do enzymatycznego upłynniania skrobi.
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Fecal indicators resistance to antimicrobial agents present in municipal wastewater
PublikacjaIn this study removal rate of six antimicrobial compounds (belonging to folate pathway inhibitors and macrolides class) was analyzed in processes of local wastewater treatment plant (WWTP) together with susceptibility patterns of erythromycin (E) resistant Enterococcus spp. and trimethoprim/sulfamethoxazole (SXT) resistant Escherichia coli. According to the obtained data, six of tested antimicrobial agents, namely erythromycin,...
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Atimicrobial Resistance of Enterococcus spp. in Muncipal wasterwater Treatment Plant - Model Study
PublikacjaIn this study the removal of resistant Enterococcus spp. was evaluated using the laboratory-scale wastewater treatment model plant (M-WWTP), designed as a multiphase (anaerobic, anoxic and aerobic) system (Q = 27 dm3 per day) and continuously supplied with the wastewater obtained from the full-scale WWTP, after grid. The activated sludge from the secondary clarifier was recirculated to the anaerobic chamber (with the ratio equal...
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Alternative treatment approaches of urinary tract infections caused by uropathogenic Escherichia coli strains
PublikacjaUrinary tract infections (UTIs) are the most widespread and annoying infections affecting millions of people every year annually. The biggest problem of urinary diseases are recurrences, the increasing resistance of uropathogens to commonly used antibiotics, as well as the high health care costs of afflicted persons. Uropathogenic Escherichia coli strains (UPECs) are the most dominant etiologic agent of community-acquired infections...
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Antimicrobial resistance of fecal indicators in disinfected wastewater
PublikacjaThe main objective of the study was to assess the potential of three systems (UV irradiation, ozonation, and micro/ultrafiltration) operated in a pilot scale in removal of antimicrobial-resistant fecal bacteria from secondary effluent of the local wastewater treatment plant (700,000 population equivalent). The effectiveness of the processes was analysed using the removal ratio of fecal indicators (Escherichia coli and Enterococcus...
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Antimicrobial resistance of fecal indicators in municipal wastewater treatment plant
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Prediction of Wastewater Quality at a Wastewater Treatment Plant Inlet Using a System Based on Machine Learning Methods
PublikacjaOne of the important factors determining the biochemical processes in bioreactors is the quality of the wastewater inflow to the wastewater treatment plant (WWTP). Information on the quality of wastewater, sufficiently in advance, makes it possible to properly select bioreactor settings to obtain optimal process conditions. This paper presents the use of classification models to predict the variability of wastewater quality at...
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Model based evaluation of plant improvement at a large wastewater treatment plant (WWTP)
PublikacjaIn this work, the effect of the improvement carried out at a large-scale wastewater treatment plant (WWTP) was evaluated, by means of modelling works, with the aim to determine the influence of the modernization over the process performance. After modernization, the energy consumption due to the aeration decreased about a 20% maintaining the effluent quality. In order to double-check the good effluent quality, modelling works were...
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Supervised model predictive control of wastewater treatment plant
PublikacjaAn optimizing control of a wastewater treatment plant (WWTP), allowing for cost savings over long time period and fulfilling effluent discharge limits at the same time, requires application of advanced control techniques. Model Predictive Control (MPC) is a very suitable control technology for a synthesis of such a truly multivariable controller that can handle constraints and accommodate model-based knowledge combined with hard...
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New Approaches for Escherichia coli Genotyping
PublikacjaEasy-to-perform, fast, and inexpensive methods of differentiation of Escherichia coli strains beyond the species level are highly required. Herein two new, original tools for genotyping of E. coli isolates are proposed. The first of the developed method, a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) test uses a highly variable fliC gene, encoding the H antigen as a molecular target. The designing...
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Influence of landfill leachate on municipal wastewater treatment - model plant(WWTP-MP)
PublikacjaAbstract In the current study, wastewater treatment model plant (WWTP-MP) was used to investigate the possibilities of landfill leachate co-treatment together with municipal wastewater. The WWT-MP, designed as an A2O multiphase system, was fed with the raw municipal wastewater (from the WWTP Gdańsk "Wschód'', Qav.=82x103 m3/d) with increasing, from 1 to 5 % (vol.), quantity of landfill leachate (collected from Gdańsk landfill ''Szadółki")....
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Use of Escherichia coli Nissle 1917 producing recombinant colicins for treatment of IBD patients
PublikacjaPatients with Crohn’s Disease and ulcerative colitis infected with Adherent-Invasive Escherichia coli strains constitute the largest group among Inflammatory Bowel Disease subjects, when taking into account all known etiological agents of the disease. A possible link between these pathogenic bacteria and inflammation process has gained the confidence in recently published papers. Observed enteric neuroglial cells apoptosis and...
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Spatial distribution of pharmaceuticals in conventional wastewater treatment plant with Sludge Treatment Reed Beds technology
PublikacjaPharmaceutical residues are an emerging environmental problem. It is strongly confirmed that pharmaceuticals are present in soils and environmental waters (surface, marine and even groundwater), and that wastewater treatment plant (WWTP) effluents are the main source of pharmaceuticals in the watershed. The aim of this studywas to recognize the spatial distribution and seasonal changes of selected pharmaceuticals in conventional WWTP...
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Respiration rate estimation using non-linear observers in application to wastewater treatment plant
PublikacjaA problem of respiration rate estimation using two new non-linear observers for a wastewater treatment plant is addressed in this paper. In particular, a non-linear adaptive Luenberger-like observer and a super twisting sliding mode observer have been derived to produce stable and bounded estimates of the respiration rate. During the synthesis of the particular observer, an appropriate mathematical utility model was used. The observability...
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Diversity of fecal coliforms and their antimicrobial resistance patterns in wastewater treatment model plant
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Characteristics of odors emitted from municipal wastewater treatment plant and methods for their identification and deodorization techniques
PublikacjaOdors emitted from municipal wastewater treatment plants belong to a group of pollutants, which is the main cause of people complaining about atmospheric air quality. The limitation of emissions of unpleasant odors generated by wastewater treatment plants by using appropriate deodorization methods is omitted on numerous occasions. This can have a negative inflence on public trust and the quality of atmospheric air. The article...
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Hydrolytic and transgalactosylation activities of immobilized Escherichia coli cells transformed by β-galactosidase gene from Pyrococcus woesei
PublikacjaPrzedstawiono wyniki badań dotyczących skuteczności transformacji laktozy katalizowanej immobilizowanymi w żelu alginianowym rekombinantowymi komórkami Escherichia coli, transformowanymi genem b-galaktozydazy z Pyrococcus woesei. Wykazano, że aktywność hydrolityczna rekombinantowych komórek wzrasta o 35% po permeabilizacji w środowisku 40% etanolu. Optymalna temperatura i pH katalizowanej reakcji wynosiły odpowiednio 98C i 5,4....
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Analysis of antibiotic resistance in Escherichia coli isolated from the Reda River and the Oliwski Stream using basic statistical methods = Zastosowanie metod ststystycznych do analizy antybiotykoodporności bakterii wskaźnikowych pochodzących z rzeki Redy i Potoku Oliwskiego
PublikacjaIn this study distribution of antimicrobial resistance patterns among fecal indicator bacteria (Escherichia coli and Enterococcus spp.) was examined in two watercourses. The susceptibility analyses were carried out against the antimicrobial agents, important in treating human E. coli and enterococcal infection. Water samples were obtained from the Oliwski Stream and from the Reda River. On each watercourse the five representative...
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Structure and the Biochemical Potential of the Bacterial Community in Wastewater Treatment Plant Effluent and in Receiving Waters: Northern Poland Case Study
PublikacjaWastewater treatment processes are monitored mainly in terms of biogenic substance removal efficiency. Little is known about the treated wastewater microbiome and its influence on the recipient. In this study, a wide range of methods (cultivation, microscopic analysis, New Generation Sequencing and Real Time PCR) were employed to determine the bacterial community composition of the treated wastewater and its receiver. Preliminary...
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Task Scheduling – Review of Algorithms and Analysis of Potential Use in a Biological Wastewater Treatment Plant
PublikacjaThe idea of task scheduling is to increase the efficiency of a system by minimising wasted time, evenly loading machines, or maximising the throughput of machines. Moreover, the use of appropriate scheduling algorithms often leads to a reduction in the energy costs of the process. Task scheduling problems are found in a variety of industrial areas, and their scale changes significantly depending on the problem. This review shows...
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Multiregional PI control strategy for dissolved oxygen and aeration system control at biological wastewater treatment plant
PublikacjaThe dissolved oxygen is a key parameter for biological processes at wastewater treatment plant. The dissolved oxygen tracking problem is one of the most complex and fundamental issue of biological processes. The paper proposes a new multiregional control strategy for dissolved oxygen tracking problem. The aeration system is included in overall control structure. The control mechanism within the operating point area is based on...
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Differences between selected volatile aromatic compound concentrations in sludge samples in various steps of wastewater treatment plant operations
PublikacjaSewage sludge, one of the main wastes generated during wastewater treatment, constitutes an important source of emissions of volatile chemical compounds such as volatile aromatic compounds These substances may undergo various changes as a result of operations and unit processes, which affects their concentrations in sewage sludge. An important factor determining the potential hazardousness of volatile organic compounds is the quality...
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Chlorinated solvents in a petrochemical wastewater treatment plant: Anassessment of their removal using self-organising maps
PublikacjaThe self-organising map approach was used to assess the efficiency of chlorinated solvent removal frompetrochemical wastewater in a refinery wastewater treatment plant. Chlorinated solvents and inorganicanions (11 variables) were determined in 72 wastewater samples, collected from three different purificationstreams. The classification of variables identified technical solvents, brine from oil desalting andrunoff sulphates as pollution...
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Expression of goose parvovirus whole VP3 protein and its epitopes in Escherichia coli cells
PublikacjaThe aim of this study was the expression of goose parvovirus capsid protein (VP3) and its epitopes in Escherichia coli cells. Expression of the whole VP3 protein provided an insufficient amount of protein. In contrast, the expression of two VP3 epitopes (VP3ep4, VP3ep6) in E. coli, resulted in very high expression levels. This may suggest that smaller parts of the GPV antigenic determinants are more efficiently expressed than the...
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Modelling of wastewater treatment plant for monitoring and control purposes by state - space wavelet networks
PublikacjaMost of industrial processes are nonlinear, not stationary, and dynamical with at least few different time scales in their internal dynamics and hardly measured states. A biological wastewater treatment plant falls into this category. The paper considers modelling such processes for monitorning and control purposes by using State - Space Wavelet Neural Networks (SSWN). The modelling method is illustrated based on bioreactors of...
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Application of subsurface vertical flow constructed wetlands to reject water treatment in dairy wastewater treatment plant
PublikacjaThe paper presents the effects of applying subsurface vertical flow constructed wetlands (SS VF) for the treatment of reject water generated in the process of aerobic sewage sludge stabilization in the biggest dairy wastewater treatment plant (WWTP) in Poland. Two SS VF beds were built: bed (A) with 0.65 m depth and bed (B) with 1.0 m depth, planted with reeds. Beds were fed with reject water with hydraulic load of 0.1 m d-1 in...
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Assessment of wastewater quality indicators for wastewater treatment influent using an advanced logistic regression model
PublikacjaInfluent quality indicators play a significant role in wastewater treatment plant performance due to their correlation with reactor operations and effluent quality. However, selecting a specific/best parameter indicator for predicting influent wastewater quality is one of the challenges in wastewa- ter treatment. This study, therefore, focused on determining suitable variables as influent quality indicators. For this purpose, a...
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Phosphorylation‐mediated regulation of heat shock response in Escherichia coli
PublikacjaEscherichia coli has two heat shock regulons under the transcriptional control of Esigma(32) and Esigma(E) RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that mutations in a tyrosine phosphatase-encoding gene led to decreased activity of these heat shock regulons. The...
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Environmental risk assessment of Polish wastewater treatment plant activity
PublikacjaWastewater treatment plants (WWTPs) play an extremely important role in shaping modern society's environmental wellbeing and awareness, however only well operated and supervised systems can be considered as environmentally sustainable. For this reason, an attempt was undertaken to assess the environmental burden posed by WWTPs in major Polish cities by collecting water samples prior to and just after wastewater release points....