Wyniki wyszukiwania dla: COLD-ADAPTED ENZYME
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ADVANCES IN ENZYME REGULATION
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Current Enzyme Inhibition
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Polish experience with cold in-place recycling
PublikacjaDeep cold in-place recycling using cement and asphalt emulsion has been used for reconstruction of existing roads since the beginning of the 1990s. This paper describes the first Polish requirements for mineral-cement-emulsion mixtures. As requirements stated for the strength of the mineral-cement-emulsion mixtures were quite high, most of the mixtures were designed using high amount of cement and aggregate added for the improvement...
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Static in vitro digestion model adapted to the general older adult population: an INFOGEST international consensus
PublikacjaUnderstanding the mechanisms of food digestion is of paramount importance to determine the effect foods have on human health. Significant knowledge on the fate of food during digestion has been generated in healthy adults due to the development of physiologically-relevant in vitro digestion models. However, it appears that the performance of the oro-gastrointestinal tract is affected by ageing and that a model simulating the digestive...
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Review and evaluation of cold recycling with bitumen emulsion and cement for rehabilitation of old pavements
PublikacjaThe article presents Polish experience with cold recycling of asphalt pavements with theusage of bituminous emulsion and cement. In the 1990s numerous roads in Polandrequired immediate reinforcement due to their significant degradation. Implementation ofthe cold recycling technology was one of the solutions to this problem. Cold recycledmixtures containebeside the recycled asphalt pavement and aggregateetwo differenttypes of binding...
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Cold Cracking Of Underwater Wet Welded S355G10+N High Strength Steel
PublikacjaWater as the welding environment determines some essential problems influencing steel weldability. Underwater welding of high strength steel joints causes increase susceptibility to cold cracking, which is an effect of much faster heat transfer from the weld area and presence of diffusible hydrogen causing increased metal fragility. The paper evaluates the susceptibility to cold cracking of the high strength S355G10+N steel used,...
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Buckling Analysis of Cold Formed Silo Column
PublikacjaThe paper is devoted to stability analysis of different models of steel cold formed silo column. The steel cylindrical silos are often composed of corrugated walls and vertical open-sectional columns uniformly placed along the silo circumference. Both the whole 3D silo, a simplified model consisting of one column with a part of the silo walls, and a single column resting on elastic foundation provided by the silo walls were analyzed....
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CLASSIFICATION OF RESTRAINTS IN THE OPTIMIZATION PROBLEM OF A COLD-FORMED PROFILE
PublikacjaThis work describes the restraints in the optimization problem. This is an important and complicated issue because it requires taking into account a vast range of information related to the design and production. In order to describe the relations of a specific optimization problem, it is essential to adopt appropriate criteria and to collect information on all kinds of restraints, i.e. boundary conditions. The following paper...
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Organic solvents aggregating and shaping structural folding of protein, a case study of the protease enzyme
PublikacjaLow solubility of reactants or products in aqueous solutions can result in the enzymatic catalytic reactions that can occur in non-aqueous solutions. In current study we investigated aqueous solutions containing different organic solvents / deep eutectic solvents (DESs) that can influence the protease enzyme's activity, structural, and thermal stabilities. Retroviral aspartic protease enzyme is responsible for the cleavage of the...
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The Influence of Cement Type on Early Properties of Cold In-Place Recycled Mixtures
PublikacjaCold in-place recycling is a commonly used maintenance treatment in rehabilitation of low and medium volume roads in Poland. Typically, two types of binding agents are used—cement and bituminous emulsion (or foamed bitumen).Due to the harsh Polish climate with many freeze/thaw cycles and frequent occurrence of saturated conditions, the used amounts of cement are higher than those commonly used in warmer parts of Europe. While there...
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Fatigue Bending of V-Notched Cold-Sprayed FeCoCrNiMn Coatings
PublikacjaCold-spray coatings were produced by FeCoCrNiMn high-entropy alloy powders deposited on carbon steel substrate. The coatings were realized at intermediate temperature and high pressure (at 1100 °C and 7 MPa). The coating microstructure was characterized by scanning electron microscopy and X-ray diffraction, revealing a very dense deposition and high flattening ratio of the splatted particles. This had a large influence on the...
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COLD CRACKING OF S460N STEEL WELDED IN WATER ENVIRONMENT
PublikacjaThis paper shows results of weldability testing of fine-grained high -strength low- alloy S460N steel welded in water environment by covered electrodes. The tests were carried out by using the CTS test specimens with fillet welds. Four specimens were welded under water and one specimen in air. Welded joints were subjected to non-destructive visual and penetration tests. The accepted joints were then subjected to macroscopic and...
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Inspired by Nature: The Use of Plant-derived Substrate/Enzyme Combinations to Generate Antimicrobial Activity in situ
PublikacjaThe last decade has witnessed a renewed interest in antimicrobial agents. Plants have received particular attention and frequently rely on the spontaneous enzymatic conversion of an inactive precursor to an active agent. Such two-component substrate/enzyme defence systems can be reconstituted ex vivo. Here, the alliin/alliinase system from garlic seems to be rather effective against Saccharomyces cerevisiae, whilst the glucosinolate/myrosinase...
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New transition curve adapted to railway operational requirements
PublikacjaThe paper points to the limited possibilities of improving the existing situation in the area of transition curves used in geometrical layouts of the railway track. Difficulties in the practical implementation and maintenance of very small horizontal ordinates of the transition curve and the ordinates of the gradient due to cant in the initial section, appearing on smooth transition curves, were indicated. The main reason for this...
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On local buckling of cold-formed channel members
PublikacjaThe paper deals with local buckling of the compressed flanges of cold-formed thin-walled channel beams subjected to pure bending or axially compressed columns. Arbitrarily shaped flanges of open cross-sections and the web-flange interactions are taken into account. Buckling deformation of a beam flange is described by displacement related to torsion of the flange about the line of its connection with the web. Total potential energy...
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Effect of dendrimer-based interlayers for enzyme immobilization on a model electrochemical sensing system for glutamate
PublikacjaIn this paper, we discuss dendrimer usage in enzyme-based electrochemical biosensors, particularly with respect to biomolecule loading on the sensing surface. A novel approach to design bioactive layers with immobilized enzymes for electrochemical biosensors using the surface plasmon resonance (SPR) method in combination with electrochemical impedance spectroscopy was presented. The gold surface was modified with linear linkers...
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Cold-Active beta-Galactosidases: Sources, Biochemical Properties and Their Biotechnological Potential
Publikacjabeta-D-Galactosidases have been studied extensively in terms of their application to a variety of industrial technologies. To date, considerable research efforts have been devoted to characterization of new cold-active beta-D-galactosidases which were isolated directly from selected species of bacteria and yeasts, as well as with the use of metagenomic approaches. This chapter will provide a review of current research towards cold-active...
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Innovative cold-formed GEB section under compression
PublikacjaCold-formed steel sections extensively affect the modern steel construction industry. Not only are they used as secondary elements like purlins or sheeting, but also they can serve as primary load-bearing members in fabricated steel panels and trusses. A new cross-section called shortly a GEB may serve as that member. However, its existence of the metal building structures industry depends on the exact, optimal dimensional parameters...
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ADAPTED PHYSICAL ACTIVITY QUARTERLY
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Application of Barkhausen method to determine internal stresses in cold-rolled and hot-rolled sheets
PublikacjaThe paper presents investigations on internal stress distribution in plane hot—or cold-rolled sheets dedicated for the cold-formed profiles. The motivation of the research is a new profile design whose bending technology, incorporating a longitudinal welded joint and hot-dip galvanization after bending and welding introduces additional internal stresses. In order to identify the entirety of stresses in the process of sheet production...
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Cold War History
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The impact of cold plasma on the phenolic composition and biogenic amine content of red wine
PublikacjaThe effect of cold plasma (CP) on phenolic compound (PC) and biogenic amine (BA) contents of red wine was investigated for the first time. The influence of CP was compared with the effects of a wine preservation using potassium metabisulfite and a combined method. The PC profile was determined by UPLC-PDA-MS/MS while BAs using DLLME-GC–MS. Chemometric analysis also was used. The content of PCs was 3.1% higher in the sample preserved...
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Stability and limit load analysis of a cold-formed channel section column
PublikacjaThe paper presents stability and limit load analysis of a steel column 1440 mm high of a cold-formed channel section, subjected to a combination of compression and bending. Experimental results were compared to the resistance of a code procedure and to the outcome of numerical non-linear analysis. Comparison was made of numerical solutions by means of static (Riks) and dynamic (Explicit and Implicit) methods. Perfectly elastic...
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The dependence of linear viscoelasticity limits of cold-recycled mixtures on time of curing and compaction method
PublikacjaCold-recycled mixtures are currently among the most widely used and investigated methods that enable recycling of old pavement structures in an environmentally friendly manner. Upon milling, the old pavement structure – whose gradation can be improved with addition of virgin aggregate – is mixed and compacted at ambient temperature. The main binding agents are bituminous emulsion and cement. Due to their dual binding behaviour,...
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Coupling of Blood Pressure and Subarachnoid Space Oscillations at Cardiac Frequency Evoked by Handgrip and Cold Tests: A Bispectral Analysis
PublikacjaThe aim of the study was to assess blood pressure–subarachnoid space (BP–SAS) width coupling properties using time–frequency bispectral analysis based on wavelet transforms during handgrip and cold tests. The experiments were performed on a group of 16 healthy subjects (F/M; 7/9) of the mean age 27.2 ± 6.8 years and body mass index of 23.8 ± 4.1 kg/m². The sequence of challenges was first handgrip and then cold test. The handgrip...
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A simple modification to improve the accuracy of methylation-sensitive restriction enzyme quantitative polymerase chain reaction
PublikacjaDNA digestion with endonucleases sensitive to CpG methylation such as HpaII followed by polymerase chain reaction (PCR) quantitation is commonly used in molecular studies as a simple and inexpensive solution for assessment of region-specific DNA methylation. We observed that the results of such analyses were highly overestimated if mock-digested samples were applied as the reference.We determined DNA methylation levels in several...
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Preliminary study of linear viscoelasticity limits of cold recycled mixtures determined in Simple Performance Tester (SPT)
PublikacjaThe publication presents methodology developed for determination of linear viscoelasticity limits for cold recycled mixtures with cement and bituminous emulsion using Simple Performance Tester (SPT). Methodology was verified on reference materials (PCV and steel dummy specimens, cement concrete and asphalt concrete) to comply with elasticity and viscoelasticity theory. The developed methodology enabled determination of linear viscoelasticity...
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The optimization of enzyme immobilization at Au-Ti nanotextured platform and its impact onto the response towards glucose in neutral media
PublikacjaThe market of non-invasive glucose sensors is drastically growing due to increasing number of people suffering from diabetes. Therefore, there is a significant need for any improvement in the field of biosensors that can be used for monitoring glucose level in human body. In recent years the emphasis is put onto the modification of electrode material with enzymes possessing recognition centre specific towards particular molecules....
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Natural ventilation performance of family building in cold climate during windy days
PublikacjaAdequately designed natural ventilation is the cheapest and easiest way to effectively remove indoor pollutants and keep the fresh air inside a building. A prediction of performance and effectiveness of ventilation in order to determine the design of a ventilation system can provide real and long term cost savings. The paper presents results of performance (air change rate ACH) and effectiveness (CO2 concentration in the breathing...
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Natural ventilation performance of family building in cold climate during windless time
PublikacjaIn this paper, the field measurements results are presented on the stack ventilation effect. The paper presents the results of performance (air change rate ACH) natural ventilation for building with inlet gap measured for the transitional season (between heating and summer season). The measurements were performed during a windless time. The house was located in northern Poland, in a cold climate region. The measurement system...
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Fouling mechanisms in anoxic-aerobic sequencing batch membrane bioreactor based on adapted Hermia models and main foulant characteristics
PublikacjaVarious derivatives of Hermia models (complete pore blocking, intermediate pore blocking, cake layer formation, and standard pore blocking) and different assessments of foulant characteristics have long been used to determine the membrane fouling mechanisms. Accordingly, this study aims to adapt Hermia models and their combination according to the operating conditions of an anoxic-aerobic sequencing batch membrane bioreactor (A/O-SBMBR)....
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Novel Cold Crucible Ultrasonic Atomization Powder Production Method for 3D Printing
Publikacja: A new powder production method has been developed to speed up the search for novel alloys for additive manufacturing. The technique involves an ultrasonically agitated cold crucible installed at the top of a 20 kHz ultrasonic sonotrode. The material is melted with an electric arc and undergoes pulverization with standing wave vibrations. Several different alloys in various forms, including noble and metallic glass alloys, were...
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Glucosamine-6-phosphate synthase - the multi-facets enzyme
PublikacjaAmidotransferaza L-glutamina: D-frukozo-6-fosforan, nazywana zwyczajowo syntazą glukozamino-6-fosforanu, jako jedyny enzym należący do grupy amidotransferaz, nie wykazuje aktywności amoniakozależnej. Enzym ten, katalizujący pierwszy wyjątkowy etap w szlaku metabolicznym prowadzącym do ostatecznego wytworzenia 5´difosfourydyno-N-acetylo-D-glukozaminy (UDG-GlcNAc) stanowi ważny punkt kontroli metabolicznej biosyntezy makromolekuł...
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Simulation of large thrust-bearing performance at transient states, warm and cold start-up
PublikacjaThe start-up of a large hydrodynamic thrust bearing of a vertical hydrogenerator is one of the most critical situations during the lifetime of a bearing. Hydrodynamic load capacity is low due to low speed and higher thermal deformations of the pad. A new approach to the simulation of a hydrodynamic bearing is shown in this article. A combination of finite elements method and computational fluid dynamics is used to perform the transient...
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European Journal of Adapted Physical Activity
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USER MODELING AND USER-ADAPTED INTERACTION
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TRANSFORMATION OF WORLD ECONOMY AFTER THE END OF COLD WAR - Nowy
Kursy OnlineThe course focuses the main trends of the world economy in the last 30 years. National and macro-regional, as well as sectorial case studies will be used to offer a clearer understanding of the main global dynamics. The approach is multidisciplinary and mainly qualitative, including economic and business history, international political economy, economic sociology and political science.
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Effect of long-term cold storage on physicochemical attributes and bioactive components of kiwi fruit cultivars
PublikacjaVarious kiwi fruit cultivars, bred in Korea, were kept in cold storage for 8–24 weeks for possible increase of their quality. Firmness significantly decreased at initial time in all cultivars.The rate of softeningwas the slowest in “Hayward”, followed by “Hort16A”, “Haenam”, “Daheung”, “Bidan”, “Hwamei”, and “SKK 12”. Sensory value increased with decreasing of firmness. Soluble solids content increased with storage time while acidity gradually...
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Local buckling of compressed flange of cold-formed channel members made of aluminum alloy
PublikacjaThe paper deals with local buckling of a compressed single flange of thin-walled channel cold- formed columns and beams made of aluminum alloy. Material is described by means of the Ramberg-Osgood constitutive equation. Axial compression of the columns and beams undergoing bending is taken into consid- eration. A simple model of the member flange in the form a long beam elastically connected to the web is used to find the critical...
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Buckling and initial post-local buckling behaviour of cold-formed channel member flange
PublikacjaThe initial post-buckling behaviour of a cold-formed channel member flange after its local buckling is investigated. An axially compressed column or beam subjected to pure bending is considered. The member material is assumed to follow a linear stress-strain relationship. The governing non-linear differential equation of the problem is derived using the minimum total potential energy principle. An approximate solution for the equation...
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Strain-dependent behaviour of cold recycled material mixtures in cyclic compression tests
PublikacjaThe purpose of the study is to evaluate strain-dependent behaviour of Cold Recycled Material (CRM) mixtures using three frameworks typically applied for Hot Mix Asphalt (HMA): linear viscoelastic (LVE) limits; non-linearity directions; changes in rheological model parameters. To this aim, the complex modulus of three CRM mixtures with different bitumen-to-cement ratio (0.2, 0.6 and 1.8) was measured in cyclic compression mode,...
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Comparative wear test of journal sliding bearings with sintered bronze and Babbitt alloy bushes lubricated by environmentally acceptable/adapted lubricants (EAL)
PublikacjaA growing awareness of the negative effects of mineral oils on the natural environment has resulted in the introduction of new regulations related to environmental protection. One of these regulations requires the use of environmentally acceptable/adapted lubricants (EAL) to lubricate marine main shaft bearings, in place of the mineral lubricating oils that have been used for decades. Classification Societies, which supervise...
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Normal stress distribution in built‑up cold‑formed column in relation to interconnecting bolt spacing
PublikacjaIn order to increase a stiffness of cold-formed steel (CFS) elements it is practised to built-up the cross-section. In the analysed case, a main element is strengthened by adding extra chord in contact partially along the column. This additional chord acts as a longitudinal stiffener connected with the main section by series of bolts. Authors check whether rules applied over the years, for hot-rolled elements, can be indiscriminately...
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The influence of combination of binding agents on fatigue properties of deep cold in-place recycled mixtures in Indirect Tensile Fatigue Test (ITFT)
PublikacjaThe publication presents fatigue properties of cold recycled mixtures for eight combinations of binding agents (cement and bituminous emulsion). Cold recycled mixtures were evaluated in Indirect Tensile Fatigue Test (ITFT) at the temperature of 20 C in controlled stress mode. As a function of horizontal stress, fatigue life is strongly influenced by combination of the binding agents. When fatigue life is analyzed as a function...
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Numerical simulation of cold flow and combustion in a swirl stabilized combustor
PublikacjaA numerical simulation model was developed to investigate the cold flow and combustion using Ansys FLUENT 2021R1. The governing equations were solved using the pressure-based method, and pressure–velocity coupling was performed using the SIMPLE method. To model the turbulent process, the RSM model was used. Non-premixed model is chosen to solve the chemical kinetics between fuel and oxigen. Radiation heat transfer was calculated...
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COLD REGIONS SCIENCE AND TECHNOLOGY
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Journal of Cold War Studies
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Sciences in Cold and Arid Regions
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Cold Spring Harbor Protocols
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Research in Cold and Arid Regions
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