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Cloning, expression in Komagataella phaffii, and biochemical characterization of recombinant sequence variants of Pseudomonas sp. S9 GDSL-esterase
PublikacjaTwo recombinant Komagataella phaffii (formerly Pichia pastoris) yeast strains for production of two sequential variants of EstS9 esterase from psychrotolerant bacterium Pseudomonas sp. S9, i.e. αEstS9N (a two-domain enzyme consisting of a catalytic domain and an autotransporter domain) and αEstS9Δ (a single-domain esterase) were constructed. However, only one of recombinant K. phaffii strains, namely Komagataella phaffii X-33/pPICZαestS9Δ,...
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Cloning, expression, and purification of a recombinant cold-adapted β-galactosidase from antarctic bacterium Pseudoalteromonas sp. 22b
PublikacjaAntarktyczny szczep, gram-ujemnych bakterii Pseudoalteromonas sp. 22b, wyizolowanych z przewodu pokarmowego kryla z gatunku Thyssanoessa macrura, jest producentem wewnątrzkomórkowej zimnolubnej beta-galaktozydazy. Białko to jest homotetramerem, w którym każda z jednostek monomeru zbudowana jest z 1028 reszt aminokwasowych. Gen kodujący ten enzym został sklonowany i eksprymowany w komórkach E. coli. Następnie z zastosowaniem procedury...
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The influence of combination of binding agents on fatigue properties of deep cold in-place recycled mixtures in Indirect Tensile Fatigue Test (ITFT)
PublikacjaThe publication presents fatigue properties of cold recycled mixtures for eight combinations of binding agents (cement and bituminous emulsion). Cold recycled mixtures were evaluated in Indirect Tensile Fatigue Test (ITFT) at the temperature of 20 C in controlled stress mode. As a function of horizontal stress, fatigue life is strongly influenced by combination of the binding agents. When fatigue life is analyzed as a function...
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Comparative study of a bottoming SRC and ORC for Joule–Brayton cycle cooling modular HTR exergy losses, fluid-flow machinery main dimensions, and partial loads
PublikacjaEnergy conversion efficiency increase in power plants with high-temperature gas-cooled reactors via implementation of the bottoming cycle was investigated under nominal and minimal thermal load of a high-temperature reactor (HTR). Heat transfer surface area and turbine outlet volumetric flow rate in bottoming cycles was also investigated. Water and two low-boiling point working fluids (ammonia and ethanol) were analyzed. Analyzed...
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Bond graph modeling of the new generation engine cooling systems = Zastosowanie metody grafów wiązań do modelowania nowej generacji układów chłodzenia silników spalinowych
PublikacjaW referacie szczegółowo opisano modele wymiany ciepła i przepływów w układzie chłodzenia. Metoda grafów wiązań okazała się szczególnie użyteczną dla modelowania skomplikowanych systemów energetycznych o różnych postaciach energii. Jako przykładu użyto obiegu chłodzenia silnika badawczego na hamowni silnikowej.
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Wpływ zaburzeń geometrii na proces wypierania w przestrzeni pierścieniowej otworów wiertniczych
PublikacjaIzolacja stref zbiornikowych, przenoszenie części naprężeń oraz spowolnienie tempa erozji rur okładzinowych to główne zadania płaszcza cementacyjnego. Powstaje on na skutek stopniowego wyparcia płuczki przez ciecze buforowe, a następnie cieczy buforowych przez zaczyn cementowy. Niepowodzenie tej operacji skutkować może katastrofą na miarę wycieku ropy w Zatoce Meksykańskiej w 2010 roku. Mimo że problem niewystarczającego związania płaszcza...
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THE USE OF FEM FOR DETERMINATION OF RESONANT FREQUENCIES OF CIRCULAR SAW BLADES WITH INDIRECT TEETH IN GULLETS
PublikacjaUnderstanding the dynamical properties of the circular saw blade is necessary, since, there is a really need for a stable work at working rotational speeds, which are determined by frequencies at which the circular saw blade tends to vibrate. It was observed that the resonant frequency depend on the circular saw blade shape, a collar diameter and on saw’s teeth shape. In the presented work an attention was paid to the effect of...
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MEDIA ARCHITECTURE AND INTERACTIVE ART INSTALLATIONS STIMULATING HUMAN INVOLVEMENT AND ACTIVITIES IN PUBLIC SPACES
PublikacjaThis paper examines the potential for media architecture and interactive art installations to stimulate human involvement and activities in public spaces. On the basis of theoretical approaches, case studies and interdisciplinary surveys, the paper provides insight into how screens projecting media and interactive installations in city spaces can inspire people to become active on many levels. The research is focused, both on temporary...
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Cascaded doubly fed induction generator for mini and micro power plants connected to grid
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The Influence of Siliceous Mineral Phases on the Mechanical Properties of Transitional Rocks in the Belchatow Lignite Deposit
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Sulforaphene, an isothiocyanate present in radish plants, inhibits proliferation of human breast cancer cells
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Quantitative structure–retention relationships of ionic liquid cations in characterization of stationary phases for HPLC
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The application of Local Indicators for Categorical Data (LICD) to explore spatial dependence in archaeological spaces
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Mechanisms shaping the synergism of zeaxanthin and PsbS in photoprotective energy dissipation in the photosynthetic apparatus of plants
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Low temperature removal of SO2 traces from air by MgO-loaded porous carbons
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Sustainable polylysine conversion to nitrogen‐containing porous carbon flakes: Potential application in supercapacitors
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Acute Effects of Caffeinated Chewing Gum on Volleyball Performance in High-Performance Female Players
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Well-Designed Porous Graphene Flakes for Lithium-Ion Batteries with Outstanding Rate Performance
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Contribution of Strength, Speed and Power Characteristics to Change of Direction Performance in Male Basketball Players
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Psoriasis and Atherosclerosis—Skin, Joints, and Cardiovascular Story of Two Plaques in Relation to the Treatment with Biologics
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Morphochemical investigation on the enrichment and transformation of hazardous elements in ash from waste incineration plants
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PhageScore-based analysis of Acinetobacter baumannii infecting phages antibiotic interaction in liquid medium
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A selection of the optimal eluent components for separation of carnivorous plants metabolites by planar chromatography chromatography
PublikacjaAbstract: The development of the new procedures of the separation components with the complicated chemical structure from natural complex matrix by column chromatography is very difficult and in many cases expensive. Therefore to pre-select the conditions of the separation process the use of thin layer chromatography (TLC) is proffered. The possibility of proceeding the chromatographic process for several mixtures on single plate,...
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Some Common Medicinal Plants with Antidiabetic Activity, Known and Available in Europe (A Mini-Review)
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Universal Modular Permanent Magnet Synchronous Generator for Small Low-Head Hydropower Plants
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Methodological issues of functional safety and reliability assessment of critical systems in industrial hazardous plants
PublikacjaThe aim of this article is to identify and discuss some methodological issues that are of interest among functional safety specialists and experts after publication of the second edition of international standards IEC 61508 and IEC 61511, including the design and implementing the safety-related functions of higher safety integrity levels and protection layers. The basic role of safety-related systems is to reduce effectively and...
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Human Factors and Functional Safety Analysis in Designing the Control Rooms of Industrial Hazardous Plants
PublikacjaIn this work some aspects of human factors and functional safety analysis in designing the control rooms of industrial hazardous plants are presented. The “defence in depth” (D-in-D) concept is outlined. Some designing issues of the alarm system (AS) with regard to human factors to be supported using the human reliability analysis (HRA) are discussed. The AS and decision support system (DSS), when properly designed, would contribute...
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New approaches to cutting resistance estimation of wood cutting process with circular-saw blades
PublikacjaTheoretical and experimental determination of values of cutting resistance belongs to the basic and simultaneously the most developed field of mechanics of this process. Energetic effects of wood sawing process are mainly calculated on the basis of the specific cutting resistance which, in wood cutting, is a function on a lot of factors. In this paper new method for calculation of cutting resistance is described. The latter method...
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THE INFLUENCE OF DIFFERENT VEGETABLE DISPERSION PHASES ON THE ROLLING CONTACT FATIGUE OF BIODEGRADABLE LUBRICATING GREASES
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20+ years of Polish residential real estate prices – a house price index proxy
PublikacjaInformation on house prices is often considered crucial when assessing developments in economy. However, in Poland for the long time house prices haven’t been tracked in an organized manner. Recent developments, most notably house price indices developed by Poland’s national bank and by the statistical office do not go back more than 10 years, therefore data series are relatively short compared to those of other macroeconomic data. In...
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Nauka w świecie cyfrowym okiem młodego inżyniera – proces i techniki uczenia
PublikacjaW obecnym świecie, z uwagi na wszechobecność cyfrowych urządzeń, odtwarzaczy multimedialnych itp., trudno jest skupić uwagę na pojedynczej czynności. Z kolei wzrost dostępnych źródeł informacji powoduje niekiedy rozproszenie i problemy z koncentracją. Naturalne wydaje się skorzystanie z urządzenia osobistego, które posiada niemal każdy z nas, czyli smartfona, w celu poprawy koncentracji i systematycznej pracy. W niniejszym artykule...
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Cadmium in edible plants from Silesia, Poland, and its implications for health risk in populations
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Plants of the Genus Terminalia: An Insight on Its Biological Potentials, Pre-Clinical and Clinical Studies
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Low temperature CVD growth of graphene nano-flakes directly on high K dielectrics
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Expedited EM-driven multi-objective antenna design in highly-dimensional parameter spaces
PublikacjaA technique for low-cost multi-objective optimization of antennas in highly-dimensional parameter spaces is presented. The optimization procedure is expedited by exploiting fast surrogate models, including coarse-discretization EM antenna simulations and response surface approximations (RSA). The latter is utilized to yield an initial set of Pareto non-dominated designs which are further refined using response correction methods....
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Natural radioactivity of solid and liquid phases from shale oil and gas prospection in Pomerania
PublikacjaIonizing radiation intensity data and estimation of equivalent absorbed dose from solid and liquid samples from prospection of shale gas and oil at selected sites in Pomerania are presented. Naturally occurring radioactive materials (NORM) are associated with geological formations or fluids and can be enhanced during oil and gas production. They appear in e.g. drilling cuttings, flow back water, produced water, scale and sludge....
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EM-Driven Multi-Objective Optimization of Antenna Structures in Multi-Dimensional Design Spaces
PublikacjaFeasible multi-objective optimization of antenna structures is presented. An initial set of Pareto optimal solutions is found using a multi-objective evolutionary algorithm (MOEA) working with a fast surrogate antenna model obtained by kriging interpolation of coarse-discretization EM simulation data. To make the surrogate construction computationally feasible in multi-dimensional design space, the space subset containing non-dominated...
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Carnivorous plants used for green synthesis of silver nanoparticles with broad-spectrum antimicrobial activity
PublikacjaIn this study, we exploit the anti-oxidative potential of four carnivorous plants to produce uniform and biologically active silver nanoparticles. The use of polyvinylpyrrolidone promoted syn-thesis of quasi-spherical nanoparticles characterized by stability and high uniformity. Their activity was tested against three human pathogens and three species of plant pathogenic bacteria. The study demonstrates the influence of synthesis...
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Nuclear retention of pre-mRNA involving Cajal bodies during meiotic prophase in plants
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Spectral Finite Element Method for Propagation of Elastic Waves in Wind Turbine Blades for SHM
PublikacjaW pracy przedstawiono model łopaty turbiny wiatrowej zbudowany z wykorzystaniem spektralnych elementów skończonych. Omówiono wyniki symulacji propagacji fal sprężystych i ich interakcji z uszkodzeniami w formie pęknięcia i delaminacji.
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On the Fenchel–Moreau conjugate of G-function and the second derivative of the modular in anisotropic Orlicz spaces
PublikacjaIn this paper, we investigate the properties of the Fenchel–Moreau conjugate of G-function with respect to the coupling function c(x, A) = |A[x]2 |. We provide conditions that guarantee that the conjugate is also a G-function. We also show that if a G-function G is twice differentiable and its second derivative belongs to the Orlicz space generated by the Fenchel–Moreau conjugate of G then the modular generated by G is twice differentiable...
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Stimulation of antibacterial napthoquinones and flavonoids accumulation in carnivorous plants grown in vitro by addition of elicitors
PublikacjaRośliny owadożerne (D. muscipula, D. capensis) zawierają dwie grupy farmakologicznie czynnych substancji - naftochinony: plumbagina, ramentaceon, oraz flawonoidy: kwercetyna i mirycetyna. Na wzrost akumulacji tychże substancji w tkankach roślin owadożernych ma wpływ dodatek elicytora. Zbadano wpływ następujących elicytorów na zawartość metabolitów o znaczeniu farmaceutycznym: kwas jaśminowy oraz lizat Agrobacterium rhizogenes....
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Characteristics and fate of organic nitrogen in municipal biological nutrient removal wastewater treatment plants
PublikacjaThe aim of this study was to investigate the occurrence and fate of colloidal and dissolved organic nitrogen (CON and DON) across biological nutrient removal (BNR) activated sludge bioreactors. Primary and secondary effluent total nitrogen (TN) measurements and component fractionation, CON and DON concentration profiles across BNR bioreactors, and laboratory batch experiments with the process mixed liquor were carried out at several...
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Wpływ niejednorodności materiału na rozrzut paraetrów energetycznych opisujących proces zmęczenia wysokocyklowego
Publikacja...
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AN INNOVATIVE APPROACH TO PREDICTION ENERGETIC EFFECTS OF WOOD CUTTING PROCESS WITH CIRCULAR-SAW BLADES
PublikacjaIn the classical approach, energetic effects (cutting forces and cutting power) of wood sawing process are generally calculated on the basis of the specific cutting resistance, which is in the case of wood cutting the function of more or less important factors. The aim of the paper is to present a new calculating model using the application of modern fracture mechanics and to compare cutting parameters of native beech, Bendywood...
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Local properties of the solution set of the operator equation in Banach spaces in a neighbourhood of a bifurcation point.
PublikacjaW niniejszej pracy badamy problem istnienia bifurkacji w zbiorze rozwiązań równania F(x,p)=0, gdzie F jest odwzorowaniem klasy C^2z iloczynu kartezjańskiego X i R^k do Y, X i Y są przestrzeniami Banacha takimi, że X jest podprzestrzenią liniową Y. Co więcej, dany jest iloczyn skalarny w Y, ciągły względem norm w X i Y. Pokazujemy, że pod pewnymi warunkami (0,p) jest punktem bifurkacji i opisujemyzbiór rozwiązań równania F(x,p)=0...
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Occurrence and seasonal variations of 25 pharmaceutical residues in wastewater and drinking water treatment plants
PublikacjaThousands of tons of pharmaceuticals are introduced into the aqueous environment due to their incomplete elimination during treatment process in wastewater treatment plants (WWTPs) and water treat- ment plants (WTPs). The presence of pharmacologically active compounds in the environment is of a great interest because of their potential to cause negative effects. Furthermore, drugs can undergo different pro- cesses leading to the...
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Cost-efficient multi-objective design optimization of antennas in highly-dimensional parameter spaces
PublikacjaMulti-objective optimization of antenna structures in highly-dimensional parameter spaces is investigated. For expedited design, variable-fidelity EM simulations and domain patching algorithm are utilized. The results obtained for a monopole antenna with 13 geometry parameters are compared with surrogate-assisted optimization involving response surface approximation modeling.
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Post-match recovery profile of leukocyte cell subsets among professional soccer players
PublikacjaThis study assessed the impact of cumulative match time on the distribution of CD45+ cell subtests in the capillary blood of professional soccer players. Twenty-two males (aged 18–30 years) took part in the 36-week study. Participants playing up to 540 in cumulative match time and less than 30 min in each single match during the observation period formed the control group. White blood cell (WBC) phenotyping and creatine kinase...
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Management of the transition spaces - comparison of the East European, West European and North American examples
PublikacjaArtykul dotyczy zagadnienia zagospodarowania przestrzeni stykowych pomiędzy strukturami portowymi i miejskimi w miastach portowych. Opisano w nim szereg przygkładów oraz zarysowano możliwe strategie zarządznaia tymi obszarami..