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Wyniki wyszukiwania dla: UKRAINA
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Escherichia coli K-12 suppressor-free mutants lacking early glycosyltransferases and late acyltransferases. Minimal lipopolysaccharide structure and induction of envelope stress response.
PublikacjaTo elucidate the minimal lipopolysaccharide (LPS) structure needed for the viability of Escherichia coli, suppressor-free strains lacking either the 3-deoxy-d-manno-oct-2-ulosonic acid transferase waaA gene or derivatives of the heptosyltransferase I waaC deletion with lack of one or all late acyltransferases (lpxL/M/P) and/or various outer membrane biogenesis factors were constructed. Δ(waaC lpxL lpxM lpxP) and waaA mutants exhibited...
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Assembly of Lipopolysaccharide in Escherichia coli Requires the Essential LapB Heat Shock Protein
PublikacjaHere, we describe two new heat shock proteins involved in the assembly of LPS in Escherichia coli, LapA and LapB (lipopolysaccharide assembly protein A and B). lapB mutants were identified based on an increased envelope stress response. Envelope stress responsive pathways control key steps in LPS biogenesis and respond to defects in the LPS assembly. Accordingly, the LPS content in ΔlapB or Δ(lapA lapB) mutants was elevated, with...
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Why is TASK Quarterly a Significant Journal to Publish Your Article? —A Bibliometric Analysis of a Scientific and Technical Journal
PublikacjaA bibliometric analysis of TASK Quarterly in the years 1997-2021 in terms of various bibliometric indicators was performed to celebrate the 25th anniversary of the publication of the first issue of the journal. The number of publications and citations increased over the mentioned span of years. The leading countries in terms of the greatest number of papers published in TASK Quarterly are Poland, Italy, Germany, Ukraine, USA and...
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e-Technologie w Kształceniu Inżynierów – otwarte pytania o MOOC w zdalnej edukacji
PublikacjaJeżeli chcemy dobrze i skutecznie uczyć, nie obędzie się bez głębokich zmian zarówno na poziomie myślenia o dydaktyce akademickiej, jak i o metodach jej rozwoju. 30 kwietnia 2015 r. odbyła się na Politechnice Gdańskiej druga edycja Konferencji „e Technologie w Kształceniu Inżynierów”. Wśród ponad 120 uczestników Konferencji mieliśmy przyjemność gościć między innymi przedstawicieli ponad dwudziestu...
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The Opening of Central and Eastern European Countries to Free Trade: A Critical Assessment
PublikacjaThree decades after the fall of the Berlin wall and one and a half decades after the Big Bang enlargement of the European Union (2004-2007), we revisit contrasting narratives about the benefit of both free trade and the EU enlargement for Central and Eastern European (CEE) countries. We distinguish old, pre-2004 EU countries from CEE countries that joined the EU in 2004-2007, as well as from the CEE countries that have not become...
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Representing and Managing Experiential Knowledge with Decisional DNA and its Drimos® Extension
PublikacjaThe Semantic Web concept is proposing a future concept of the WorldWideWeb (WWW) where both humans and man-made systems are able to interconnect and exchange knowledge. One of the challenges of Semantic Web is smart and trusted accommodation of knowledge in artificial systems so it can be unified, enhanced, reused, shared, communicated and distributed with added aptitude. Our research represents an important component of addressing...
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Identification of Substrates of Cytoplasmic Peptidyl-Prolyl Cis/Trans Isomerases and Their Collective Essentiality in Escherichia Coli
PublikacjaProtein folding often requires molecular chaperones and folding catalysts, such as peptidyl-prolyl cis/trans isomerases (PPIs). The Escherichia coli cytoplasm contains six well-known PPIs, although a requirement of their PPIase activity, the identity of their substrates and relative enzymatic contribution is unknown. Thus, strains lacking all periplasmic and one of the cytoplasmic PPIs were constructed. Measurement of their PPIase...
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Multicopy Suppressor Analysis of Strains Lacking Cytoplasmic Peptidyl-Prolyl cis/trans Isomerases Identifies Three New PPIase Activities in Escherichia coli That Includes the DksA Transcription Factor
PublikacjaConsistent with a role in catalyzing rate-limiting step of protein folding, removal of genes encoding cytoplasmic protein folding catalysts belonging to the family of peptidyl-prolyl cis/trans isomerases (PPIs) in Escherichia coli confers conditional lethality. To address the molecular basis of the essentiality of PPIs, a multicopy suppressor approach revealed that overexpression of genes encoding chaperones (DnaK/J and GroL/S),...
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Dissection of σE‐dependent cell lysis in Escherichia coli: roles of RpoE regulators RseA, RseB and periplasmic folding catalyst PpiD
PublikacjaTo understand the mechanism of sigma(E)-dependent cell lysis, we examined the consequences of deletion derivatives of rpoE regulators rseA, rseB and rseC on sigma(E) transcription, on levels of free versus membrane-bound sigma(E) and on OMP-biogenesis limiting factor(s) that could impact cell lysis. RT-PCR showed that individual nonpolar DeltarseA and DeltarseB increased the rpoE expression to varying extents, with pronounced induction...
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Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
PublikacjaThe RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional...
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Prognozowanie mocy skrawania przy przecinaniu polskiego drewna sosnowego na pilarkach tarczowych
PublikacjaPrzedstawiono metodę określania mocy skrawania dla pilarek tarczowych wykorzystującą w modelu elementy mechaniki pękania. W obliczeniach uwzględniano wiązkość materiału obrabianego oraz jego wytrzymałość na ścinanie. Wykazano, że wspomniane parametry są silnie uzależnione od pochodzenia drewna. Dla jednej operacji przeprowadzono obliczenia dla drewna sosnowego pochodzącego z 4. różnych Krain geograficznych Polski.
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Prognozowanie mocy skrawania przy przecinaniu polskiego drewna sosnowego na pilarkach tarczowych
PublikacjaPrzedstawiono metodę określania mocy skrawania dla pilarek tarczowych wykorzystującą w modelu elementy mechaniki pękania. W obliczeniach uwzględniano wiązkość materiału obrabianego oraz jego wytrzymałość na ścinanie. Wykazano, że wspomniane parametry są silnie uzależnione od pochodzenia drewna. Dla jednej operacji przeprowadzono obliczenia dla drewna sosnowego pochodzącego z 4. różnych Krain geograficznych Polski.
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Partnerstwo wschodnie - osiągnięcia i wyzwania po czterech latach współpracy
PublikacjaPartnerstwo Wschodnie stanowi pierwszą koncepcję polityki Unii Europejskiej wobec regionu Europy Wschodniej i Kaukazu Południowego. Podjęta próba oceny dotyczyła postępów w realizacji celów Partnerstwa Wschodniego w kontekście efektywności unijnej polityki. Po czterech latach funkcjonowania inicjatywy nie może być ona ani pełna ani jednoznaczna ze względu na krótki okres, mnogość determinantów, w tym rozproszenie unijnego wpływu...
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Molecular and structural basis of inner core lipopolysaccharide alterations in Escherichia coli: incorporation of glucuronic acid and phosphoethanolamine in the heptose region.
PublikacjaIt is well established that lipopolysaccharide (LPS) often carries nonstoichiometric substitutions in lipid A and in the inner core. In this work, the molecular basis of inner core alterations and their physiological significance are addressed. A new inner core modification of LPS is described, which arises due to the addition of glucuronic acid on the third heptose with a concomitant loss of phosphate on the second heptose. This...
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Suppressors of lapC mutation identify new regulators of LpxC, which mediates the first committed step in lipopolysaccharide biosynthesis
PublikacjaGram-negative bacteria, such as Escherichia coli, are characterized by an asymmetric outer membrane (OM) with lipopolysaccharide (LPS) located in the outer leaflet and phospholipids facing the inner leaflet. E. coli recruits LPS assembly proteins LapB, LapC and LapD in concert with FtsH protease to ensure a balanced biosynthesis of LPS and phospholipids. We recently reported that bacteria either lacking the periplasmic domain of...
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Review of Research into Enterprise Bankruptcy Prediction in Selected Central and Eastern European Countries
PublikacjaIn developed countries, the first studies on forecasting bankruptcy date to the early 20th century. In Central and Eastern Europe, due to, among other factors, the geopolitical situation and the introduced economic system, this issue became the subject of researcher interest only in the 1990s. Therefore, it is worthwhile to analyze whether these countries conduct bankruptcy risk assessments and what their level of advancement is....
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Exploring the cocrystallization potential of urea and benzamide
PublikacjaThe cocrystallization landscape of benzamide and urea interacting with aliphatic and aromatic carboxylic acids was studied both experimentally and theoretically. Ten new cocrystals of benzamide were synthesized using an oriented samples approach via a fast dropped evaporation technique. Information about types of known bi-component cocrystals augmented with knowledge of simple binary eutectic mixtures was used for the analysis...
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Applicability of phenolic acids as effective enhancers of cocrystals solubility of methylxanthines
PublikacjaApplicability of phenolic acids as potential cocrystal formers for methylxanthine derivatives was analyzed both in terms of cocrystallization probabilities and solubility advantage. The cocrystal formation abilities were evaluated using mixing enthalpy estimated within the conductor like screening model for real solvents (COSMO-RS) framework. The solubility improvement of potential cocrystals was estimated by formulation of the...
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Propensity of salicylamide and ethenzamide cocrystallization with aromatic carboxylic acids
PublikacjaThe cocrystallization of salicylamide (2-hydroxybenzamide, SMD) and ethenzamide (2-ethoxybenzamide, EMD) with aromatic carboxylic acids was examined both experimentally and theoretically. The supramolecular synthesis taking advantage of the droplet evaporative crystallization (DEC) technique was combined with powder diffraction and vibrational spectroscopy as the analytical tools. This led to identification of eleven new cocrystals...
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Review of International Standards and Policy Guidelines for Smart Sustainable Cities
PublikacjaSmart cities are often criticized for preoccupation with technology, for ignoring the negative effects of technology, for irrelevance to the needs of the poor, and for ubiquitous data collection creating perfect conditions for surveillance societies and autocratic states. In response, cities pursue smartness and sustainability simultaneously, becoming global (by participation in global digital networks) and local (by addressing...
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The effect of cabbage juices on the activity and expression of GST isozymes in HepG2 cells
PublikacjaWcześniejsze badania pokazały zdolność soków z kapusty białej do indukowania aktywności enzymów detoksykacyjnych. Prezentowane wyniki potwierdziły zdolność fitokompleksu kapusty do stymulacji aktywności enzymów z grupy GST w komórkach HepG2 - modelu ludzkiej wątroby - w szczególności izoenzymów GST-alfa i GST-mi.
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A new factor LapD is required for the regulation of LpxC amounts and lipopolysaccharide trafficking
PublikacjaLipopolysaccharide (LPS) constitutes the major component of the outer membrane and is essential for bacteria, such as Escherichia coli. Recent work has revealed the essential roles of LapB and LapC proteins in regulating LPS amounts; although, if any additional partners are involved is unknown. Examination of proteins co-purifying with LapB identified LapD as a new partner. The purification of LapD reveals that it forms a complex...
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Ciliated protozoa in the impact zone of the Uzhgorod treatment plant
PublikacjaThis paper presents the results of studies on the Uzh River (Ukraine, Zakarpattia Oblast) near the effluent point of a sewage treatment plant in Uzhgorod. The samples were taken at various sites of the treatment plant along the stages of purification process, as well as in the river, at a number of different points above and below the wastewater discharge. At each of these objects, the temperature...
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WAYS TO IMPROVE THE EFFICIENCY OF WASTEWATER TREATMENT OF A CARDBOARD AND PAPER MILL
PublikacjaThe results of research on physical and chemical methods for the preliminary treatment of wastewater of a cardboard and paper factory in Khmelnytskyi region of Ukraine are presented. At the cardboard and paper factory, wastewater is treated at a sewage treatment plant, which includes sand traps, primary radial sedimentation tanks, aeration tanks with activated sludge regenerators, secondary radial sedimentation tanks, and bioponds....
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Fabrication and Characterization of Green Polyurethane Foams with Enhanced Vibration Damping Capability
PublikacjaOver the past decades, there has been an intensive development of the technologies and innovations aimed at increasing the quality of life. This development largely concerns the comfort of use of everyday products that, among others, are products of the automotive, construction, or furniture industries. Considering the increase in the comfort of using products such as cars, houses, and flats, one of the comfort features of their...
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Entrepreneurial competencies and intentions among students of technical universities
PublikacjaThe study investigates the entrepreneurial competencies and intentions of students. Their formation is a requirement of modern times. The question arises whether students of modern technical universities get entrepreneurial competencies and whether they transform these competencies into their entrepreneurial intentions. More than 3.6 thousand students from six technical universities from Poland, Ukraine, Latvia, Bulgaria, and Lithuania...
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Transformation of the Odesa Waterfront
PublikacjaOdesa is a unique city: it is the third-largest city in Ukraine and the second in the Black sea region. During its short but eventful history, it gathered a variety of cultural layers within its boundaries, what had reflected in its architecture, urban life, and unique accent of its inhabitants. The city`s waterfront is considered to be the Pearl of the Black Sea. Starting with the receiving of the Porto Franco status in 1819,...
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Experimental and theoretical solubility advantage screening of bi-component solid curcumin formulations
PublikacjaA comprehensive experimental and theoretical screening was performed for identification of curcumin solubilizers. Experimental data led to formulation of a non-linear QSPR model correlating molecular descriptors with measured solubilities. The majority of synthesized binary systems exhibited a moderate enhancement of curcumin solubility, which was found to be the highest in the case of curcumin cocrystallized with pyrogallol. New...
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Utilization of oriented crystal growth for screening of aromatic carboxylic acids cocrystallization with urea
PublikacjaThe possibility of molecular complex formation in the solid state of urea with benzoic acid analogues was measured directly on the crystallite films deposited on the glass surface using powder X-ray diffractometry (PXRD). Obtained solid mixtures were also analyzed using Fourier transform infrared spectroscopy (FTIR). The simple droplet evaporation method was found to be efficient, robust, fast and cost-preserving approach for first...
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The surface photoconductivity in molecular layers
PublikacjaW pracy przedstawiono rezultaty analizy fotoprądów powierzchniowych w cienkich warstwach tetracenu i pentacenu. W szczególności badano wpływ obecności defektów strukturalnych w warstwach na charakterystyki fotoprądów i transport nośników ładunku.
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Youth attitude to entrepreneurship in Eastern and Central European countries: Gender aspect
PublikacjaCurrent business conditions pose new challenges to youth entrepreneurship, which is a significant component of countries’ economic growth. In addition, Generation Z differs from previous generations and requires new approaches. In this context, a comprehensive study of the peculiarities and various aspects of youth entrepreneurship development is highly-demanded and relevant. Furthermore, the lower representation of women among...
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Ts2631 Endolysin from the Extremophilic Thermus scotoductus Bacteriophage vB_Tsc2631 as an Antimicrobial Agent against Gram-Negative Multidrug-Resistant Bacteria
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Ts2631 Endolysin from the Extremophilic Thermus scotoductus Bacteriophage vB_Tsc2631 as an Antimicrobial Agent against Gram-Negative Multidrug-Resistant Bacteria
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Creation of Hydrogen Bonded 1D Networks by Cocrystallization of N,N`-bis(2-pyridyl) aryldiamines with Dicarboxylic Acids.Tworzenie 1D sieci krystalicznych poprzez kokrystalizację N,N` -bis(2-piry- dylo) arylodiamin z kwasami dikarboksylowymi.
PublikacjaZsyntetyzowano szereg N,N`-bis(2-pirydylo) arylodiamin, a następnie otrzymano serię kompleksów w/w amin z kwasami dikarboksylowymi oraz kwasem kwadratowym w postaci monokryształów. Jednostki N,N`-bis(2-pirydylo) arylodiamin i kwasy dikarboksylowe oddziaływują ze sobą poprzez wiązania wodorowe tworząc ośmioczłonowy cykliczny układ. W kompleksach 1:1 cząsteczki układają się w jedno-wymiarową sieć krystaliczną tworzoną przy udziale...
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Decreased levels of 6-tetrahydrobiopterin in psoriatic epidermis
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FOREIGN TRADE WITH CHINA: NO REASON FOR “GERMAN ANGST”
PublikacjaSankcje w związku z rosyjską agresją zbrojną przeciwko Ukrainie i naruszenie zwykłych światowych szlaków handlowych powodują w Niemczech obawy, że niemiecka gospodarka może zostać szantażowana handlem z silnym partnerem, tj. Chinami.Analiza danych dotyczących niemiecko-chińskiego handlu zagranicznego i jego wpływu na gospodarkę i zatrudnienie pokazuje, że Chiny są jednym z nielicznych...
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Experimental and computational study on mechanical behaviour of carpentry corner log joints
PublikacjaThis work concerns experimental and numerical research on carpentry joints used in historic wooden buildings in southeastern Poland and western Ukraine. These structures are mainly sacred buildings, and the types of corner log joints characteristic of this region are primarily saddle-notch and dovetail joints; thus, these two types of joints were analysed in this study. The modelling of historic timber structures is a complex...
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Application of Multivariate Adaptive Regression Splines (MARSplines) Methodology for Screening of Dicarboxylic Acids Cocrystal Using 1D and 2D Molecular Descriptors
PublikacjaDicarboxylic acids (DiAs) are probably one of the most popular cocrystals formers. Due to the high hydrophilicity and non-toxicity, they are promising solubilizes of active pharmaceutical ingredients (APIs). Although DiAs appear to be highly capable of forming multicomponent crystals with various compounds, some systems reported in the literature are physical mixtures the solid state without forming stable intermolecular complex....
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A bibliometric analysis of the recent achievements in pulmonary safety of nanoparticles
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Investigation on stability of mountainous EUPOS sites’ coordinates
PublikacjaThe paper concerns analysis of solutions obtained during common processing of data from GNSS permanent stations situated on mountainous terrain: the Western Carpathians, the Sudetes Mountains and adjacent areas. As the outcome daily and weekly solutions (ellipsoidal coordinates) of forty Polish, Czech, Slovak, Ukrainian and German sites were obtained. Weekly solutions were used to determine velocity field and vertical movements,...
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Chemiczne "czary-mary" i Harry (I)
PublikacjaZa sprawą książek o Harrym Potterze (ale nie tylko) nasz świat zapełnił się latającymi miotłami, magicznymi zaklęciami, cudownymi eliksirami i czarodziejami. Wszystko to - dzięki swej obrazowości - z łatwością trafia do młodego pokolenia. Które dziecko nie chciałoby się na chwilę przeistoczyć w tytułowego bohatera powieści Joanne Kathleen Rowling i przenieść się do szkoły czarów? "Zwykła" lekcja chemii może stać się zaproszeniem...
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Interaction of the conserved region 4.2 of sigma(E) with the RseA anti-sigma factor
PublikacjaEo-E RNA polymerase transcribes a regulon of folding factors for the bacterial envelope and is induced by physical and chemical stresses. The RseA anti-sigma factor inhibits the activity of Esigma(E) RNA, polymerase. It is shown here that the N-terminal portion of sigma(E), residues 1-153, binds core RNA polymerase. RseA interacts with residues 154-191 of sigma(E), a site that is homologous to region 4, the sigma factor binding...
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Wydatki związane z organizacją EURO 2004 i źródła ich finansowania
PublikacjaW kontekście organizacji turnieju finałowego przez Polskę i Ukrainę szczególnie interesująca wydaje się analiza przypadku EURO 2004 i próba przeniesienia portugalskich doświadczeń na grunt Polski. Pod wieloma względami obecna sytuacja naszego kraju przypomina to, co było udziałem tego niewielkiego państwa z Półwyspu Iberyjskiego w okresie przygotowawczym przed Mistrzostwami Europy.W niniejszym opracowaniu największe znaczenie przypisuje...
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Psoriasis and Atherosclerosis—Skin, Joints, and Cardiovascular Story of Two Plaques in Relation to the Treatment with Biologics
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Highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances specificity of simplex and multiplex PCR assays
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The influence of fresh cabbage and sauerkraut juices on the activity of protective enzymes in human cell lines
PublikacjaMechanizmami przeciwrakotwórczego działania związków zawartych w kapuście jest m.in. metaboliczna aktywacja enzymów II fazy detoksykacji, indukcja enzymów naprawczych DNA oraz aktywnośc przeciwutleniająca. Do enzymów II fazy odtruwania należą m.in. S-transferazy glutationowe (GST) oraz oksydoreduktaza chinonowa NQO1 zwana też DT-diaforazą. GST katalizują reakcję sprzęgania glutationu z wieloma różnymi typami ksenobiotyków, ułatwiając...
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Phyloproteomic study by MALDI-TOF MS in view of intraspecies variation in a significant homogenous phytopathogen Dickeya solani
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Paid work activity and entrepreneurial cognitions of students – evidence from European emerging economies
PublikacjaPurpose – This paper aims to investigate whether paid work activity (PWA) experience of students from five emerging economies is related to academic results and self-assessment of possessed entrepreneurial traits. Additionally, the authors verify the relationship between obtaining work experience and the willingness to start own business among students. Design/methodology/approach – Participants included 3,631 students of the first...
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Implementing artificial intelligence in forecasting the risk of personal bankruptcies in Poland and Taiwan
PublikacjaResearch background: The global financial crisis from 2007 to 2012, the COVID-19 pandemic, and the current war in Ukraine have dramatically increased the risk of consumer bankruptcies worldwide. All three crises negatively impact the financial situation of households due to increased interest rates, inflation rates, volatile exchange rates, and other significant macroeconomic factors. Financial difficulties may arise when the...
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Crystal structures of the DsbG disulfide isomerase reveal an unstable disulfide
PublikacjaDsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. Here, we present two crystal structures of DsbG at 1.7- and 2.0-Angstrom resolution that are meant to represent the reduced and oxidized forms, respectively. The oxidized structure, however, reveals a mixture...