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Lipidated Analogs of the LL-37-Derived Peptide Fragment KR12—Structural Analysis, Surface-Active Properties and Antimicrobial Activity
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Urine metabolic fingerprinting using LC–MS and GC–MS reveals metabolite changes in prostate cancer: A pilot study
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Metabolic Evaluation of Urine from Patients Diagnosed with High Grade (HG) Bladder Cancer by SPME-LC-MS Method
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LC-PDA-MS and GC-MS Analysis of Scorzonera hispanica Seeds and Their Effects on Human Breast Cancer Cell Lines
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Direct injection liquid chromatography-mass spectrometry (DI-LC-MS) analysis for rapid lipidomic profiling of extracellular vesicles
PublikacjaExtracellular vesicles (EVs) are small, spherical particles produced by eukaryotic and prokaryotic cells, surrounded by a bilayer membrane and carrying various bioactive molecules, such as proteins, surface receptors, membrane and soluble proteins, lipids, and nucleic acids. EVs are of substantial interest because of their important roles in cell communication, epigenetic regulation and possible application in disease diagnosis...
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LC-MS/MS dtermination of isoprostanes in plasma samples collected from mice exposed to doxorubicin or tert-butyl hydroperoxide
PublikacjaIsoprostanes are stable products of arachidonic acid peroxidation and are regarded as the most reliable markers of oxidative stress in vivo. Here we describe the LC-MS/MS procedure enabling simultaneous determination of four regioisomers (8-iso prostaglandin F2α, 8-iso-15(R)-prostaglandin F2α, 11β-prostaglandin F2α, 15(R)-prostaglandin F2α) in plasma samples collected from mice. The four plasma isoprostanes are determined by LC–ESI-MS/MS...
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Measure small currents without adding resistive insertion loss
PublikacjaFloating current to voltage converter can be inserted in-line with no voltage drop and no need for a ground reference.
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An analysis of solar energy conversion systems based on photon and thermal processes
PublikacjaSolar spectral irradiance covers a fairly broad wavelength range. Solar radiation is part of the electromagnetic spectrum which is described by the concept of wave-particle duality. The corpuscular theory of electromagnetic radiation states that energy is transmitted by photons. Photons carry specific amounts of energy which can be used to convert solar energy into other types of energy, in particular electricity. The internal...
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Badania nad rozdzielaniem grupowym technikami sorpcji i chromatografii. Część I – Wykorzystanie chromatografii cienkowarstwowej (TLC) dla doboru warunków rozdzielania tłuszczów i produktów ich konwersji technikami kolumnowymi.
PublikacjaPrzeprowadzono badania wyjaśniające wpływ eluentu oraz fazy stacjonarnej na wartość parametrów elucji mono-, di-, triacylogliceroli oraz wolnych kwasów tłuszczowych, eluowanych w warunkach cienkowarstwowej chromatografii cieczowej (TLC). Celowo wykorzystano sorbenty inne niż kopolimer styrenu i di-winylobenzenu (SDVB). Starano się dobrać taki eluent, aby możliwe było rozdzielanie tłuszczów za pomocą chromatografii wykluczania...
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Power Electronics Building Blocks for implementing Smart MV/LV Distribution Transformers for Smart Grid
PublikacjaWith an observed increase in the involvement of active consumers in activities aimed at improving energy efficiency and increasing interest in producing energy from renewable sources, there is a need for the development of new technologies enabling the distribution network operators to offer new services and functionalities. Smart MV/LV distribution transformers are characterized by a compact three-stage design, including an...
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Positron collisions with molecular hydrogen: cross sections and annihilation parameters calculated using theR-matrix with pseudo-states method
PublikacjaThe molecular R-matrix with pseudo-states (MRMPS) method is employed to study positron collisions with H2. The calculations employ pseudo-continuum orbital sets containing up to h (l = 5) functions. Use of these high l functions is found to give converged eigenphase sums. Below the positronium formation threshold, the calculated cross sections agree with other high-accuracy theories and generally with the measurements. Calculation...
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Adaptive Optimal Discrete-Time Output-Feedback Using an Internal Model Principle and Adaptive Dynamic Programming
PublikacjaIn order to address the output feedback issue for linear discrete-time systems, this work suggests a brand-new adaptive dynamic programming (ADP) technique based on the internal model principle (IMP). The proposed method, termed as IMP-ADP, does not require complete state feedback, merely the measurement of input and output data. More specifically, based on the IMP, the output control problem can first be converted into a stabilization...
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A Novel Biological Role for Peptidyl-Arginine Deiminases: Citrullination of Cathelicidin LL-37 Controls the Immunostimulatory Potential of Cell-Free DNA
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Cross-Lingual Knowledge Distillation via Flow-Based Voice Conversion for Robust Polyglot Text-to-Speech
PublikacjaIn this work, we introduce a framework for cross-lingual speech synthesis, which involves an upstream Voice Conversion (VC) model and a downstream Text-To-Speech (TTS) model. The proposed framework consists of 4 stages. In the first two stages, we use a VC model to convert utterances in the target locale to the voice of the target speaker. In the third stage, the converted data is combined with the linguistic features and durations...
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Experimental test results of the 150kVA 18-pulse diode rectifier with series active power filter
PublikacjaThe paper presents the control system and selected results of experimental tests of the ac/dc power converter consisting of 18-pulse diode rectifier on the basis of coupled reactors and a serial active power filter. Three configurations of the 150kVA converter were tested experimentally and compared: 18-pulse diode rectifier with and without additional grid reactor and the rectifier integrated with the active power filter. The...
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Design of battery charging system update for glider launcher
PublikacjaThis paper presents an innovative solution for increasing life of lead-acid batteries used in a glider launcher. The study is focused on upgrading a charging system instead of a costly full replacement of it. Based on literature review, the advanced three-stage charging profile was indicated. The new topology of the power converter was proposed and a simulation model was developed. A simulation study was performed which leads to...
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A new dilution-enrichment sample preparation strategy for expanded metabolome monitoring of human breast milk that overcomes the simultaneous presence of low- and high-abundance lipid species
PublikacjaThe complex nature of human breast milk (HBM) makes samples difficult to analyze, requiring several extraction techniques and analytical platforms to obtain high metabolome coverage. In this work, we combined liquid-liquid extraction (LLE) and solid-phase extraction (SPE) techniques to prepare HBM samples to overcome the challenge of low- and high-abundance lipid species, enabling the semiquantitative analysis of total HBM lipids...
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Dielectric Barrier Discharge Systems with HV Generators and Discharge Chambers for Surface Treatment and Decontamination of Organic Products
PublikacjaThe article presents applications of systems with power electronic converters, high voltage transformers, and discharge chambers used for nonthermal, dielectric barrier discharge plasma treatment of a plastic surface and decontamination of organic loose products. In these installations, the inductance of the high voltage transformers and the capacitances of the electrode sets form resonant circuits that are excited by inverters....
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Performance of the PEM fuel cell module. Part 2. Effect of excess ratio and stack temperature
PublikacjaThe paper describes a fuel cell based system performance under different thermal conditions. The system could be fed with bottled hydrogen or with very high purity hydrogen obtained from reforming of methanol. The system is based on two fuel cell units (1.2 kW each, produced by Ballard Power Systems Inc. and called Nexa), DC/DC converter, DC/AC inverter, microprocessor control unit, load unit, bottled hydrogen supply system and...
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Time-domain measurement methods for R, L and C sensors based on aversatile direct sensor-to-microcontroller interface circuit
PublikacjatIn the paper new time-domain measurement methods for determining values of resistive (R), inductive(L) and capacitive (C) sensors based on a versatile direct sensor-to-microcontroller interface for microcon-trollers with internal analog-to-digital converters (ADCs) and analog comparators (ACs) are presented.The interface circuit consists of a reference resistor Rrworking as a voltage divider, a given R, L or C sensorand a microcontroller...
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Decentralized control of a different rated parallel UPS systems
PublikacjaThe paper presents the single phase uninterruptible power supply (UPS) system with galvanic separated DC-AC-DC-AC converters operating in parallel. The CAN physical layer based system of communication between converters has been developed and applied, which allow to utilize a decentralized master-slave control providing high availability factor of the whole UPS system. The control system of particular converters has been developed...
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Investigating Feature Spaces for Isolated Word Recognition
PublikacjaThe study addresses the issues related to the appropriateness of a two-dimensional representation of speech signal for speech recognition tasks based on deep learning techniques. The approach combines Convolutional Neural Networks (CNNs) and time-frequency signal representation converted to the investigated feature spaces. In particular, waveforms and fractal dimension features of the signal were chosen for the time domain, and...
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Extraction and Analysis of Bisphenols and Their Derivatives in Infant and Toddler Ready-to-feed Meals by Ultrasound-assisted Membrane Extraction Followed by LC MS/MS
PublikacjaThis research developed an ultrasound-assisted membrane extraction coupled with liquid chromatography-tandem mass spectrometry method for the determination of nineteen bisphenols and their derivatives in infant and toddler ready-to-feed meals. The calibration curves for all analytes were linear in the tested range, and the limit of detection and limit of quantitation were in the range 0.27 to 0.79 ng/g and 0.80 to 2.4 ng/g, respectively....
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Probing the binding selected metal ions and biologically active substances to the antimicrobial peptide LL-37 using DSC, ITC measurements and calculations
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Determining pesticide contamination in honey by LC-ESI-MS/MS - Comparison of pesticide recoveries of two liquid-liquid extraction based approaches
PublikacjaHoney, a valuable food product, may be contaminated by xenobiotics during its production and/or harvest. The determination of trace levels of contaminants in a complex matrix like honey still presents a challenge to analytical chemists. The aim of this work was to assess and compare the extraction efficiencies of 30 pesticide residues (acaricides, insecticides, herbicides, fungicides), belonging to over 15 different chemical classes....
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NARAŻENIA NAPIĘCIOWE SILNIKÓW INDUKCYJNYCH ZASILANYCH Z FALOWNIKÓW NAPIĘCIA
PublikacjaW artykule przedstawiono zagadnienia związane z narażeniami izolacji silników pracujących w układach napędowych z falownikami napięcia. Znaczne stromości napięć oraz zjawiska falowe w przewodach zasilających powodują przyspieszoną degradacje izolacji maszyn. Rozwiązaniem jest stosowanie filtrów biernych na wyjściu falownika.
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Application of wireless communication to small WECS with induction generator
PublikacjaWind energy conversion systems (WECS) seem to be self-evident elements of the future smart grids. Among many generator types the squirrel cage induction generator (SCIG) characterizes robustness and low cost. A full-scale power converter can perform smooth grid connection over wide speed range of multiple-stage geared SCIG. Increasing number of sensors can improve the WECS control and diagnostics but increasing number of thin wires...
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Electrochemical oxidation of PFOA and PFOS in landfill leachates at low and highly boron-doped diamond electrodes
PublikacjaPolyfluorinated alkyl substances (PFASs) may reach landfill leachates (LLs) due to improper waste management. In this study perfluorooctanoate (PFOA) and perfluorooctane sulphonate (PFOS) were used as representatives of PFASs in the decomposition on boron-doped diamond electrodes (BDDs) with high (10k ppm) and low (0.5k ppm) boron doping concentrations. The result shows that although better COD removal efficacies are obtained on...
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A method for a comprehensive lipidomic analysis of flaxseed (Linum usitatissimum) with the use of LC–Q-TOF-MS and dispersive micro-solid-phase (μDSPE) extraction
PublikacjaFlaxseed (FS) is one of the richest sources of α-linolenic acid oil and lignans, and it is suggested that the consumption of flaxseed may contribute to the prevention of certain chronic diseases such as many types of cancer, diabetes, cardiovascular diseases and cerebrovascular stroke. Here, we demonstrate a new method for comprehensive FS lipidome profiling with the use of LC–Q-TOF-MS and dispersive micro-solid-phase extraction....
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A comprehensive lipidomic analysis of oilseeds using LC-Q-TOF-MS and dispersive micro-solid phase (D-μ-SPE) extraction techniques
PublikacjaGrowing public health awareness increased the demand for natural products with positive health benefits. Vegetable oils containing omega-3 fatty acids (FA) such as alpha-linolenic acid (ALA) has been proposed as a possible source of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). In this work, a comprehensive lipidome analysis and comparison of seven oilseeds, including flaxseed, chia, hemp, black cumin, white sesame,...
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Concentration of Potentially Bioactive Compounds in Italian Extra Virgin Olive Oils from Various Sources by Using LC-MS and Multivariate Data Analysis
PublikacjaHigh quality extra virgin olive oils represent an optimal source of nutraceuticals. The European Union (EU) is the world’s leading olive oil producer, with the Mediterranean region as the main contributor. This makes the EU the greatest exporter and consumer of olive oil in the world. However, small olive oil producers also contribute to olive oil production. Beneficial effects on human health of extra virgin olive oil are well...
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Determination of non-steroidal anti-inflammatory drugs in natural waters using off-line and on-line SPE followed by LC coupled with DAD-MS
PublikacjaOpisano dwie metody pozwalające na jednoczesne oznaczanie zawartości niesterydowych leków przeciwzapalnych (difluisal, diklofenak, naproksen, tolmetin, fenoprofen, ibuprofen) w próbkach wodnych. Próbki przygotowywano do analizy z użyciem techniki ekstrakcji do fazy stałej (off-line i on-line). Natomiast oznaczeń końcowych dokonano z użyciem LC-DAD-MS. Sprawdzono kilka różnych kolumienek ekstrakcyjnych i wybrano RP-18 jako najlepsze...
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Exploring the potential of green chemistry in reversed-phase liquid chromatography: A review of sustainable solvents
PublikacjaThis article delves into the growing field of green analytical chemistry, focusing on sustainable solvents for reversed-phase liquid chromatography (RP LC). RP LC is a widely applied technique in the pharmaceutical, food, and environmental analysis industries, consuming vast quantities of toxic and non-renewable solvents that pose serious environmental and health risks. In response to the urgent need for greener alternatives, this...
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Probing Local Structural Changes by Sharp Luminescent Infrared Nanophosphor for Application in Light-Emitting Diodes
PublikacjaCr3+-doped infrared phosphors are promising candidates for next-generation phosphor-converted infrared light-emitting diodes (LEDs) because they can, in principle, tune and convert the luminescence spectra from an LED chip. However, most studies focus on broad-band Cr3+-doped phosphors, and the control mechanism of Cr3+-doped phosphors with sharp line emissions remains ambiguous. Here, we report LiGa5(1–x)Al5xO8:Cr3+ phosphors...
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Power Hardware-In-the-Loop Approach for Autonomous Power Generation System Analysis
PublikacjaThe article presents the Power Hardware-In-the-Loop (PHIL) dynamic model of a synchronous generator of 125 kVA for autonomous power generation system analysis. This type of system is typically composed of electrical energy sources in the form of several diesel generator units with synchronous machines, the main distribution switchboard and different loads. In modern power distribution systems, the proposed power management strategies...
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Techno-economic analysis of a hybrid energy system for electrification using an off-grid solar/biogas/battery system employing HOMER: A case study in Vietnam
PublikacjaThe electrification of off-grid /island villages is a critical step towards improving the techno-economic circumstances of rural regions and the overall general growth of the country. However, consistent supply from a single source is not possible in these areas. Thus, a hybrid renewable energy system performs better in these conditions. The research challenge now is to identify the optimal combinations of HRES from the available...
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Analiza i badania transformatora hybrydowego z przekształtnikiem AC/DC/AC współdziałającym z przełącznikiem zaczepów
PublikacjaRozprawa dotyczy badań nad układem transformatora hybrydowego, który oprócz możliwości regulacji ciągłej napięcia za pomocą przekształtnika energoelektronicznego, posiada układ regulacji skokowej w postaci przełącznika zaczepów. Badane urządzenie przeznaczone jest do stabilizacji napięcia w dystrybucyjnej sieci niskiego napięcia. Dzięki skoordynowanej pracy układów (ciągłego i skokowego) uzyskana zostaje tzw. Wielostrefowa Regulacja...
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Metrological analysis of an computerized system of protection against electric shock in circuits with variable speed drives
PublikacjaDrive systems, that can be operated at variable speed, are equipped with power electronics converters. This causes distortion of the earth current, and consequently the need to take into account the use of proper protective devices in relation to earth current harmonics. This paper presents a system for protection against electric shock in circuits with power electronics converters and metrological analysis indicating the requirements...
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Loss Minimization-Based Sensorless Control of High-Speed Induction Motor Considering Core Loss
PublikacjaThis paper presents loss-minimizing sensorless control (LMC) strategies utilized to optimize the energy of high-speed induction motor (HSIM) drives. A machine’s ability to operate effectively depends on the estimation of its electrical losses. Although copper losses account for the majority of electrical losses in electrical machines, core loss also contributes a major part, particularly in high-speed induction motors. A review...
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Plant-based meat substitute analysis using microextraction with deep eutectic solvent followed by LC-MS/MS to determine acrylamide, 5-hydroxymethylfurfural and furaneol
PublikacjaFor the analysis of plant-based meat substitutes and the determination of Maillard reaction products such as acrylamide, 5-hydroxymethylfurfural and furaneol, a novel and effective procedure based on hydrophobic natural deep eutectic solvent and liquid chromatography coupled with tandem mass spectrometry was developed for the first time. The 49 compositions of the deep eutectic solvents were designed and screened to select the...
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Determination of free tyrosine in equestrian supplements by LC–MS/MS and comparison of its quantity with total free amino acids content in view of doping control
PublikacjaThe reports on the probable beneficial impact of tyrosine (TYR) supplementation on performance enhancement have contributed to the growth of interest in TYR in equestrian sports field and related industries, such as the manufacture of dietary and nutritional supplements. In this study, the first attempt to the assessment of horses exposure to TYR during nutritional supplementation was demonstrated by quantification of unbound TYR...
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AC Motor Voltage and Audible Noise Dataset
PublikacjaThe dataset titled AC motor voltage and audible noise waveforms in ship’s electrical drive systems with frequency converters contains the voltage and sound measurement results recorded in a marine frequency controlled AC drive system. The dataset is part of research focussing on the impact of the ship’s electrical drive systems with frequency converters on vibrations and the level of audible noise on ships. The dataset allows the...
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Fast Real-Time RDFT- and GDFT-Based Direct Fault Diagnosis of Induction Motor Drive
PublikacjaThis paper presents the theoretical analysis and experimental verification of a direct fault harmonic identification approach in a converter-fed electric drive for automated diagnosis purposes. On the basis of the analytical model of the proposed real-time direct fault diagnosis, the fault-related harmonic component is calculated using recursive DFT (RDFT) and Goertzel DFT (GDFT), applied instead of the full spectrum calculations...
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Research of electric drive systems with real time software configurable control
PublikacjaПредмет исследования. Представлен учебно-лабораторный стенд для исследования систем управления элек- троприводами. Стенд используется для обучения студентов системам управления электроприводами и предна- значен для повышения эффективности усвоения материала. Метод. В основу предлагаемого решения положен метод взаимного нагружения электрических машин, питаемых от силовых преобразователей с общим звеном постоянного тока. Это позволяет...
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Electrochemical oxidation of landfill leachate using boron-doped diamond anodes: pollution degradation rate, energy efficiency and toxicity assessment
PublikacjaElectrochemical oxidation (EO), due to high efficiency and small carbon footprint, is regarded as an attractive option for on-site treatment of highly contaminated wastewater. This work shows the effectiveness of EO using three boron-doped diamond electrodes (BDDs) in sustainable management of landfill leachate (LL). The effect of the applied current density (25–100 mA cm−2) and boron doping concentration (B/C ratio: 500 ppm, 10,000...
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Active Power Quality Controllers
PublikacjaDynamic Static Synchronous Compensator is the most important controller for distribution networks and probably in SEEN. It has been widely used since the 1990s to regulate system voltage precisely, improve voltage profile, reduce voltage harmonics, reduce transient voltage disturbances and load compensation. Rather than using conventional capacitors and inductors combined with fast switches, the D-STATCOM uses a power-electronics...
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The effect of current delay angle on tripping of residual current devices
PublikacjaPower electronics converters applied in domestic or similar installations may utilize current delay (phase) angle control to change the level of transferred power. Due to application of such types of converters, earth fault current in the installation may be strongly distorted. The current distortion level depends on a value of current delay angle. This delay angle also influences the tripping threshold of residual current devices....
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Selectivity Tuning by Natural Deep Eutectic Solvents (NADESs) for Extraction of Bioactive Compounds from Cytinus hypocistis—Studies of Antioxidative, Enzyme-Inhibitive Properties and LC-MS Profiles
PublikacjaIn the present study, the extracts of Cytinus hypocistis (L.) L using both traditional solvents (hexane, ethyl acetate, dichloromethane, ethanol, ethanol/water, and water) and natural deep eutectic solvents (NADESs) were investigated in terms of their total polyphenolic contents and antioxidant and enzyme-inhibitive properties. The extracts were found to possess total phenolic and total flavonoid contents in the ranges of 26.47–186.13...
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Preparation and evaluation of 1,3-alternate 25,27-bis[p-nitrobenzyloxy]-26,28-bis[3-propyloxy]-calix[4]arene-bonded silica gel stationary phase for LC
PublikacjaPrzeprowadzono syntezę nowej fazy stacjonarnej na bazie sililowej pochodnej 25,27-bis[p-nitrobenzyloksy]-26,28-bis-[3-propyloksy]kaliks[4]arenu zablokowanego w konformacji 1,3-naprzemianległej. Zbadano selektywność i sprawność fazy w stosunku do szeregu klas związków organicznych techniką wysokosprawnej chromatografii cieczowej.
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A nontargeted approach to determine the authenticity of Ginkgo biloba L. plant materials and dried leaf extracts by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and chemometrics
PublikacjaThe lack of stringent regulations regarding raw materials for herbal supplements used for medicinal purposes has been a constant challenge in the industry. Ginkgo biloba L. leaf extracts attract consumers because of the supposed positive effect on mental performance and memory. Supplements are produced using dried leaf materials and standardized leaf extracts such as EGb 761. Adulteration of Ginkgo biloba L. plants and extracts...