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Wyniki wyszukiwania dla: NITRIFYING BACTERIA
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Lipopolysaccharides: regulated biosynthesis and structural diversity
PublikacjaThe cell envelope of Gram-negative bacteria contains two distinct membranes, an inner (IM) and an outer (OM) membrane, separated by the periplasm, a hydrophilic compartment that includes a thin layer of peptidoglycan. The most distinguishing feature of such bacteria is the presence of an asymmetric OM with phospholipids located in the inner leaflet and lipopolysaccharides (LPSs) facing the outer leaflet. The maintenance of this...
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Meiothermus ruber cells as β-galactosidase activity biocatalyst
PublikacjaThe current study allowed to establish the possibility of using whole cells of thermophilic bacteria Meiothermus ruber as a biocatalyst with β-galactosidase activity. β-galactosidases are used for hydrolysis of lactose as well as for oligosaccharides synthesis. The advantages of using whole bacterial cells catalysis is not only elimination of tedious, expensive protein isolation and/or purification but also stabilization of enzymes...
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Wpływ sezonowych zmian temperatury na właściwości sedymentacyjne osadu czynnego
PublikacjaPrzedstawiono badania dotyczące wpływu temperatury na właściwości sedymentacyjne osadu czynnego w dwóch komunalnych oczyszczalniach ścieków. Sezonowe zmiany opadalności osadu związane są ze zmianami temperatury oraz zmianą składu populacji bakterii osadu czynnego (bakterie nitkowate). Na rozwój tych bakterii wpływa nie tylko temperatura, lecz także intensywność napowietrzania oraz dostępność substancji pożywkowych.
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Influence of Different Nanometals Implemented in PMMA Bone Cement on Biological and Mechanical Properties
PublikacjaCemented arthroplasty is a common process to fix prostheses when a patient becomes older and his/her bone quality deteriorates. The applied cements are biocompatible, can transfer loads, and dampen vibrations, but do not provide antibacterial protection. The present work is aimed at the development of cement with antibacterial effectivity achieved with the implementation of nanoparticles of different metals. The powders of Ag,...
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Minimum inhibitory concentrations (MICs) determination of selected human topoisomerase II alpha and bacterial DNA gyrase inhibitors against fungal strains
Dane BadawczeThe datasets contain the results of determining the MIC (Minimal Inhibitory Concentration) value of known compounds* (inhibitors of human topoisomerase II alpha and bacterial DNA gyrase) against C. albicans SC5314, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 and Saccharomyces cerevisiae ATCC 9763 by the modified M27-A3...
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Structure of EstA esterase from psychrotrophic Pseudoalteromonas sp. 643A covalently inhibited by monoethylphosphonate.
PublikacjaThe crystal structure of the esterase EstA from the cold-adapted bacteriumPseudoalteromonas sp. 643A was determined in a covalently inhibited form at aresolution of 1.35 A˚. The enzyme has a typical SGNH hydrolase structureconsisting of a single domain containing a five-stranded beta-sheet, with threehelices at the convex side and two helices at the concave side of the sheet, and isornamented with a couple of very short helices...
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Analyzing and improving a mechanistic model for the Microthrix parvicella in activated sludge systems
PublikacjaW pracy przedstawiono wyniki estymacji parametrów dla mechanistycznego modelu bakterii Microthrix parvicella w systemach osadu czynnego. W tym celu wykorzystano dane z oczyszczalni ścieków w Szwajcarii oraz wyniki dodatkowych badań laboratoryjnych w reaktorze nieprzepływowym. Model właściwie prognozował szybkość poboru tlenu (mierzoną dla różnych substratów) oraz zawartść bakterii Microthrix w biomasie osadu czynnego.
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Interaction of amphotericin B and its low toxic derivative, N-methyl-N-D-fructosyl amphotericin B methyl ester, with fungal, mammalian and bacterial cells measured by the energy transfer method.
PublikacjaPochodna amfoterycyny B ( AMB) ester metylowy N-metylo-N-D-fruktozylo amfoterycyny B (MFAME) wykazuje szerokie spektrum i wysoką aktywność przeciwgrzybową wyjściowego amtybiotyku i równocześnie toksyczność mniejszą o dwa rzędy wielkości. Celem tej pracy było ustalenie czy obserwowane różnice między toksycznością pochodnej i wyjściowego antybiotyku wynikają z różnic w powinowactwie do błon komórkowych grzybów i komórek ssakowych....
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A subset of two adherence systems, acute pro-inflammatory pap genes and invasion coding dra, fim, or sfa, increases the risk of Escherichia coli translocation to the bloodstream
PublikacjaAn analysis of the phylogenetic distribution and virulence genes of Escherichia coli isolates which predispose this bacteria to translocate from the urinary tract to the bloodstream is presented. One-dimensional analysis indicated that the occurrence of P fimbriae and α-hemolysin coding genes is more frequent among the E. coli which cause bacteremia. However, a two-dimensional analysis revealed that a combination of genes coding...
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Evaluation of antimicrobial activity of porous composites based on chitosan/poly (vinyl alcohol)
Dane BadawczeThe dataset contains the results of microbiological tests of composite porous materials whose activity was assessed for their ability to reduce the number of Escherichia coli and Staphylococcus aureus strain, representing the Gram (-) and Gram (+) bacteria, respectively.
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Satish Raina prof. dr
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Estimation of autotrophic maximum specific growth rate constant-experience from the long-term operation of a laboratory-scale Sequencing Batch Reactor System
PublikacjaW pracy wykorzystano wyniki badań z długookresowej eksploatacji laboratoryjnego reaktora typu SBR do estymacji stałej szybkości przyrostu bakterii nitryfikacyjnych. Badania obejmowały zarówno pomiary empiryczne (doświadczenia przy niskim stosunku substratu do biomasy) oraz symulacje komputerowe. Do symulacji dynamicznych wykorzystano jedną wartość współczynnika = 1,2 1/d, pomimo różnych wartości mierzonych jednostkowych szybkości...
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Straightening of ship hull structure made of 316L stainless steel - tensile test of water cooled materia
Dane BadawczeThe AISI 316L type steel belongs to the group of chromium-nickel stainless steels. They are determined according to European standards as X2CrNiMo17-12-2 and belong to the group of austenitic stainless steels. Steels of this group are used for elements working in seawater environments, for installations in the chemical, paper, and food, industries,...
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Hubert Cieśliński dr hab. inż.
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Environmental characteristics of a tundra river system in Svalbard. Part 2: Chemical stress factors
PublikacjaBacterial communities in the Arctic environment are subject to multiple stress factors, including contaminants, although typically their concentrations are small. The Arctic contamination research has focused on persistent organic pollutants (POPs) because they are bioaccumulative, resistant to degradation and toxic for all organisms. Pollutants have entered the Arctic predominantly by atmospheric and oceanic long-range transport,...
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Wpływ wysokiego ciśnienia na mikroorganizmy
PublikacjaW przeglądowym opracowaniu przedstawiono wpływ wysokich ciśnień na bakterie, grzyby i wirusy. Scharakteryzowano wrażliwość mikroorganizmów na działanie wysokiego ciśnienia w zależności od typu mikroorganizmu, fazy wzrostu, składu środowiska i parametrów procesu. Omówiono czynniki warunkujące ciśnienioodporność form przetrwalnych bakterii.
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Comparative studies of the Acinetobacter genus and the species identification method based on the recA sequences
PublikacjaZaproponowano metodę genotypowania bakterii rodzaju Acinetobacter, opartą o analizę sekwencji genów recA, amplifikację fragmentów genu recA i analizę profili fragmentów restrykcyjnych po cięciu produktów reakcji PCR enzymami restrykcyjnymi (recA PCR/RFLP). Profile RFLP otrzymane za pomocą enzymu Tsp509I są użyteczne w identyfikacji poszczególnych gatunków bakterii rodzaju Acinetobacter. Na podstawie analizy otrzymanych sekwencji...
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Results of pharmaceuticals mixtures toxicity on inhibition of bioluminescence of Vibrio fischeri (Microtox)
Dane BadawczeThe research was concerned with verifying the impact of mixtures of nine pharmaceuticals against a selected organism, i.e., the bacterium Aliivibrio fischeri. A. fisheri is used as a model organism in the monitoring of acute toxicity in environmental and reference samples in Microtox® systems. Tested pharmaceuticals, namely: diclofenac (sodium salt),...
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Charakterystyka oporności Klebsiella pneumoniae New Delhi na karbapenemy
PublikacjaBakterie Klebsiella pneumoniae New Delhi, posiadające gen oporności na karbapenemy (blaNDM) zostały po raz pierwszy wyizolowane w 2008 roku w Szwecji od pacjenta wcześniej hospitalizowanego w Indiach. Bakterie te szybko rozprzestrzeniają się po całym świecie, a od 2011 roku występują również w Polsce. Stanowią one wysokie ryzyko epidemiologiczne, ponieważ gen oporności na karbapenemy przekazywany jest pomiędzy bakteriami poprzez...
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Występowanie oraz polimorfizm genów kodujących syntezę autotransporterów proteaz serynowych (SPATE) wśród bakterii Escherichia coli wywołujących biegunki u dzieci w regionie Gdańska
PublikacjaW grupie 96 szczepów bakterii Escherichia coli wyizolowanych w rejonie Gdańska od dzieci z biegunką zbadano obecność oraz polimorfizm sekwencji genów kodujących grupę białek o wspólnej nazwie: SPATE (Serine Protease Autotransporters of Enterobacteriaceae). Za pomocą analizy restrykcyjnej zidentyfikowano 8 różnych genów kodujących białka z tej grupy. Obecność jednego genu stwierdzono w 39 szczepach, w 15 szczepach występowały dwa,...
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Zakażenia bakteryjne w przewlekłym zapaleniu ucha środkowego - ocena przydatności diagnostyki molekularnej opartej o technikę PCR. Bacterial infection in chronic otitis media - assessment of usefulness of molecular diagnostic based in PCR method.
PublikacjaIdentyfikacja zakażeń w przewlekłym zapaleniu ucha środkowego jest trudna, o czym świadczą wysokie odsetki ujemnych wyników badań bakteriologicznych. Przyczyną tego jest trudność w uzyskiwaniu wiarygodnego materiału do badań oraz stosunkowo niska czułość klasycznych metod diagnostycznych. Amplifikacja bakteryjnego DNA techniką PCR jest badaniem, które może przyczynić się do zwiększenia efektywności i czułości badań bakteriologicznych....
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Biomechanical testing of bioactive bone cements – a comparison of the impact of modifiers: antibiotics and nanometals
PublikacjaApart from its bone filler and fracture stabilizing function, bone cement can be used as a carrier of bioactive substances, and such modified bone cement can protect the implant against microorganisms, treat local infections and combat bacteria introduced during the surgical procedure. In this paper, the effects of modifying antibiotics and nanosilver on the biomechanical properties of bone cement were examined. The following tests...
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Regulated Control of the Assembly and Diversity of LPS by Noncoding sRNAs
PublikacjaThe outer membrane (OM) of Gram-negative bacteria is asymmetric due to the presence of lipopolysaccharide (LPS) facing the outer leaflet of the OM and phospholipids facing the periplasmic side. LPS is essential for bacterial viability, since it provides a permeability barrier and is a major virulence determinant in pathogenic bacteria. In Escherichia coli, several steps of LPS biosynthesis and assembly are regulated by the RpoE...
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Gracjana Klein-Raina dr hab.
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Stan sanitarny wód i osadów dennych wybranych potoków w rejonach ich ujść do Zatoki Gdańskiej.
PublikacjaW pracy zaprezentowano wyniki badań bakteriologicznych prowadzonych w strefie brzegowej Zatoki Gdańskiej w rejonie Sopotu. Pracami objęto obszary oddziaływania 4 potoków uchodzących do Zatoki.Oznaczono ogólną liczbę bakterii psychrofilnych, mezofilnych, grupy coli i coli typu kałowego. Wyizolowano takie bakterie z rodziny Enterobacteriacea i przeprowadzno ich identyfikacje
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Investigation of Microbes in the Stratosphere – Stardust Project
PublikacjaThe stratospheric microbiota has been investigated many times using the methods of classical microbiology. In this research a few modern methods, including NGS sequencing and multiple displacement amplification of DNA, were in use. The analysis of metagenome helped to determine the content of various species of bacteria in the sample collected in the stratosphere by a hydrogen-filled balloon holding...
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Antimicrobial Activity of Chimera Peptides Composed of Human Neutrophil Peptide 1 (HNP-1) Truncated Analogues and Bovine Lactoferrampin
PublikacjaThree chimera peptides composed of bovine lactoferrampin and the analogue of truncated human neutrophil peptide 1 were synthesized by the solid-phase method. In two compounds peptide chains were connected via isopeptide bond, whereas in the third one disulfide bridge served as a linker. All three chimeras displayed significantly higher antimicrobial activity than the constituent peptides as well as their equimolar mixtures. The...
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Karaś P., Kochanowicz K., Pitek M., Domański P., Obuchowski I., Tomiczek B., Liberek K.: Evolution towards simplicity in bacterial small heat shock protein system// eLife -, (2023), s.1-21
PublikacjaEvolution can tinker with multi-protein machines and replace them with simpler single-protein systems performing equivalent functions in an equally efficient manner. It is unclear how, on a molecular level, such simplification can arise. With ancestral reconstruction and biochemical analysis, we have traced the evolution of bacterial small heat shock proteins (sHsp), which help to refold proteins from aggregates using either...
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The effect of high pressure on formation of volatile amines in minced meat of cod (Gadus morhua)
PublikacjaThe effects of high pressure at subzero temperature (193 MPa, at −20 °C) on inactivation of natural microflora of cod meat and degradation of trimethylamine oxide (TMAO) to trimethylamine (TMA) during refrigerated storage and to dimethylamine (DMA) and formaldehyde (FA) during frozen storage were investigated. The content of TMA, DMA and FA in cod meat did not change immediately after pressure treatment. During 40 days of frozen...
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Regulated Assembly of LPS, Its Structural Alterations and Cellular Response to LPS Defects
PublikacjaDistinguishing feature of the outer membrane (OM) of Gram-negative bacteria is its asymmetry due to the presence of lipopolysaccharide (LPS) in the outer leaflet of the OM and phospholipids in the inner leaflet. Recent studies have revealed the existence of regulatory controls that ensure a balanced biosynthesis of LPS and phospholipids, both of which are essential for bacterial viability. LPS provides the essential permeability...
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Biological and mechanical properties of bone cement with nanoparticles - in vivo and in vitro research
PublikacjaDespite antibiotics preventive treatment, before and after an implant implementation, risk of infection are real. These infections at the implant surface develop in a few months after applying them into the body. To prevent the development of bacteria and to reduce the risk of infection, implants coated with nanoparticles come into use. The Mechanical Department of the Technical University of Gdańsk carried out a research with...
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Analysis of the impact of wastewater discharge on recipients: synergistic approach
PublikacjaThe dissertation presents the analysis of chemical and microbial composition of wastewater and examines the impact of wastewater discharge on the environment. Various case studies were analysed: from small settlements in a pristine European Arctic, to large municipal wastewater treatment plants in areas subjected to greater anthropogenic pressure. In order to comprehensively analyse the impact of wastewater discharge on the receiving...
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Abnormal cytological findings - Female, 34 - Cell image [426073003847831]
Dane BadawczeThis is the histopathological image of CERVIX UTERI cell sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Efficiency of landfill leachate treatment in a MBR/UF system combined with NF, with a special focus on phthalates and bisphenol A removal
PublikacjaIn this study, a pilot-scale membrane bioreactor (MBR) was operated at a municipal solid waste plant (MSWP) to treat a mixture of landfill leachates (LLs) obtained from modern (MP-LLs) and previous (PP-LLs) waste cells. The MBR unit combined anoxic and aerobic zones with external ultra- and nanofiltration (MBR/UF and MBR/UF/NF, respectively). In addition to the removal of macropollutants, special attention was given to phthalates...
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Changes of microbiological quality of water in distribution system. Zmiany mikrobiologicznej jakości wody w sieci dystrybucyjnej.
PublikacjaW artykule przedstawiono mikrobiologiczną jakość wody podziemnej uzdatnianej w dwóch stacjach oczyszczania wody (WTP I i WTP II), w sieci dystrybucyjnej. Obie stacje uzdatniania stosują dezynfekcję za pomocą dwutlenku chloru. Badania wykazały wzrost ogólnej liczby bakterii w miarę zwiększania się odległości od stacji uzdatniania.In the paper microbiological quality of water in distribution system produced in two ground water treatment...
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Microbiological analysis of sea water and bottom sediments in Kosakowo – Gdańsk profile
Dane BadawczeThis dataset contains the results of microbiological analysis of sea water and bottom sediments in Kosakowo – Gdańsk profile in 2013 - 2014 . The tested material consisted of water samples (surface water and bottom water) and bottom sediment samples. Water and sediment samples were analysed for the following: number of Enterobacteriaceae genus bacteria,...
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Anthra[1,2-d][1,2,3]triazine-4,7,12(3H)-triones as a New Class of Antistaphylococcal Agents: Synthesis and Biological Evaluation
PublikacjaThe development and spread of resistance of human pathogenic bacteria to the action of commonly used antibacterial drugs is one of the key problems in modern medicine. One of the especially dangerous and easily developing antibiotic resistant bacterial species is Staphylococcus aureus. Anthra[1,2-d][1,2,3]triazine-4,7,12(3H)-triones 22–38 have been developed as novel effective antistaphylococcal agents. These compounds have been...
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Straightening of ship hull structure made of 316L stainless steel - tensile test of reference material materia (transverse direction)
Dane BadawczeThe AISI 316L type steel belongs to the group of chromium-nickel stainless steels. They are determined according to European standards as X2CrNiMo17-12-2 and belong to the group of austenitic stainless steels. Steels of this group are used for elements working in seawater environments, for installations in the chemical, paper, and food, industries,...
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Straightening of ship hull structure made of 316L stainless steel - tensile test of reference material materia (longitudinal direction)
Dane BadawczeThe AISI 316L type steel belongs to the group of chromium-nickel stainless steels. They are determined according to European standards as X2CrNiMo17-12-2 and belong to the group of austenitic stainless steels. Steels of this group are used for elements working in seawater environments, for installations in the chemical, paper, and food, industries,...
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Biological and mechanical properties of bone cement with nanoarticles - in vitro and in vivo research
PublikacjaDespite antibiotic preventive treatment both before and after implant implementation, the risks of infection are real. These infections develop at the implant surface a few months after inserting them into the body. To prevent the development of bacteria and reduce the risk of infection, implants coated with nanoparticles are used. The Mechanical Department of the Technical University of Gdansk carries out research into using bone...
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Thermal Instability of Choline Chloride-Based Deep Eutectic Solvents and Its Influence on Their Toxicity─Important Limitations of DESs as Sustainable Materials
PublikacjaDeep eutectic solvents (DESs) have become a hot topic in many branches of science due to their remarkable properties. They have been studied in a wide variety of applications. In particular, choline chloride (ChCl)-based DESs are one of the most commonly used representatives of these fluids. Nevertheless, in order to apply DESs in some fields, it is essential to guarantee their stability, reusability, and biocompatibility. In this...
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Biomass-Derived Nitrogen Functionalized Carbon Nanodots and Their Anti-Biofouling Properties
PublikacjaThe prevalence of the antibiotic resistant bacteria remains a global issue. Cheap, sustainable and multifunctional antibacterial membranes are at the forefront of filtrating materials capable of treating multiple flow streams, such as water cleansing treatments. Carbon nanomaterials are particularly interesting objects shown to enhance antibacterial properties of composite materials. In this article, amino-functionalized, photoluminescent...
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Results of modeling of pharmaceuticals mixtures toxicity with deviation ratio and best-fit functions models using Aliivibrio fischeri bacterium as model organism
Dane BadawczeThe research was concerned with verifying the impact of mixtures of nine pharmaceuticals against a selected organism, i.e., the bacterium Aliivibrio fischeri. A. fisheri is used as a model organism in the monitoring of acute toxicity in environmental and reference samples in Microtox® systems. Tested pharmaceuticals, namely: diclofenac (sodium salt),...
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Troianalexandrina. Anuario sobre Literatura Medieval de Materia Clasica. Journal on medieval literature containing classical material
Czasopisma -
Microorganisms in the air within the Gdańsk-Szadółki landfill and adjacent area in 2016
Dane BadawczeThe data collection contains the results of microbiological examination of air samples collected at Gdańsk Szadółki Landfill (Poland). The Gdańsk Szadółki Landfill is one of the biggest of facilities of this type in the country. It occupies an area of 75 hectares and serves around 500 thousand of local residents. The landfill receives around 250 million...
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Bateria superkondensatorów SC
Aparatura BadawczaBateria superkondensatorów SC
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Feasibility Study of Biohydrogen Production from Acid Cheese Whey via Lactate-Driven Dark Fermentation
PublikacjaThe high loading of lactic acid bacteria (LAB) present in cheese whey still limits its use as hydrogen feedstock. This study aims to investigate the feasibility of producing hydrogen from acid cheese whey via lactate-driven dark fermentation (LD-DF). Mesophilic batch fermentations were performed with delipidated acid cheese whey at a fixed pH of 5.8 and driven by an acidogenic bacterial culture containing LAB and lactate-oxidizing...
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Microorganisms in the air within the Gdańsk-Szadółki landfill and adjacent area in 2015
Dane BadawczeThis data set contains the results of microbiological examination of air samples collected at Gdańsk Szadółki Landfill (Poland) in 2015. The range of examination included qualitative and quantitative assesment of selected species of bacteria and mold fungi, with particular focus on pathogenic and potentially pathogenic fungi and bacteria. The collected...
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Microorganisms in the air within the Gdańsk-Szadółki landfill and adjacent area in 2018
Dane BadawczeThis data set contains the results of microbiological examination of air samples collected at Gdańsk Szadółki Landfill (Poland) in 2018. The range of examination included qualitative and quantitative assesment of selected species of bacteria and mold fungi, with particular focus on pathogenic and potentially pathogenic fungi and bacteria. The collected...
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Microorganisms in the air within the Gdańsk-Szadółki landfill and adjacent area in 2017
Dane BadawczeThis data set contains the results of microbiological examination of air samples collected at Gdańsk Szadółki Landfill (Poland) in 2017. The range of examination included qualitative and quantitative assesment of selected species of bacteria and mold fungi, with particular focus on pathogenic and potentially pathogenic fungi and bacteria. The collected...