Wyszukiwarka
Filtry
wszystkich: 3558
-
Katalog
- Publikacje 3167 wyników po odfiltrowaniu
- Czasopisma 117 wyników po odfiltrowaniu
- Konferencje 1 wyników po odfiltrowaniu
- Osoby 32 wyników po odfiltrowaniu
- Projekty 2 wyników po odfiltrowaniu
- Zespoły Badawcze 1 wyników po odfiltrowaniu
- Kursy Online 34 wyników po odfiltrowaniu
- Wydarzenia 8 wyników po odfiltrowaniu
- Dane Badawcze 196 wyników po odfiltrowaniu
wyświetlamy 1000 najlepszych wyników Pomoc
Wyniki wyszukiwania dla: CRISPR–CAS9
-
Establishing the allosteric mechanism in CRISPR‐Cas9
PublikacjaAllostery is a fundamental property of proteins, which regulates biochemical information transfer between spatially distant sites. Here, we report on the critical role of molecular dynamics (MD) simulations in discovering the mechanism of allosteric communication within CRISPR‐Cas9, a leading genome editing machinery with enormous promises for medicine and biotechnology. MD revealed how allostery intervenes during at least three...
-
Principles of target DNA cleavage and the role of Mg2+ in the catalysis of CRISPR–Cas9
PublikacjaAt the core of the CRISPR–Cas9 genome-editing technology, the endonuclease Cas9 introduces site-specific breaks in DNA. However, precise mechanistic information to ameliorate Cas9 function is still missing. Here, multimicrosecond molecular dynamics, free energy and multiscale simulations are combined with solution NMR and DNA cleavage experiments to resolve the catalytic mechanism of target DNA cleavage. We show that the conformation...
-
CRISPR Journal
Czasopisma -
A CRISPR/Cas9-Based Mutagenesis Protocol for Brachypodium distachyon and Its Allopolyploid Relative, Brachypodium hybridum
Publikacja -
Catalytic Mechanism of Non-Target DNA Cleavage in CRISPR-Cas9 Revealed by Ab Initio Molecular Dynamics
PublikacjaCRISPR-Cas9 is a cutting-edge genome editing technology, which uses the endonuclease Cas9 to introduce mutations at desired sites of the genome. This revolutionary tool is promising to treat a myriad of human genetic diseases. Nevertheless, the molecular basis of DNA cleavage, which is a fundamental step for genome editing, has not been established. Here, quantum–classical molecular dynamics (MD) and free energy methods are used...
-
Managing knowledge in a tourism crisis: case study from Poland
PublikacjaPurpose: This study deals with a tourism organisation from Poland, which experienced not only the COVID-19 pandemic, but also the close war situation in Ukraine which caused a significant decrease in tourist traffic and revenues. Since, based on the literature, knowledge management can be useful for crisis management, this study aims to explore the role and usefulness of KM during crisis situations in tourism. Methodology: Qualitative...
-
Crisia
Czasopisma -
Anti-crisis activities and export performance in the Covid-19 pandemic: The case of Polish exporters
PublikacjaThe article aims to investigate the impact of anti-crisis activities undertaken by the Polish exporting firms on their export sales during the Covid-19 pandemic. We used a quantitative research design. We conducted the survey on the sample of 161 manufacturing Polish exporting firms between April 21 and June 25, 2021. To verify the assumed relationships, we used the probit regression model. The main novelty is a firm-level analysis...
-
Crisis – The Journal of Crisis Intervention and Suicide Prevention
Czasopisma -
Economic Crisis in Croatia
Publikacja -
Economic crisis in Croatia
PublikacjaCroatia is becoming the 28th member of the European Union on July 1st, 2013. Croatia has gone a long way from a socialistic republic to an independent country recognized as one of the economic tigers of the Western Balkans in the first decade of the 21st century. Croatia has been hit by a global crisis which turned out to be a huge external shock for the region of the Western Balkans. Although it does not enter the economy through...
-
Illness Crisis and Loss
Czasopisma -
Boiling crisis - danger or a must?
PublikacjaW artykule przedstawiono zjawisko kryzysu wrzenia w przepływie. W głównej mierze skupiono się na zjawisku dryout'u podczas przepływu pierścieniowego. W pracy przedstawiono teoretyczny model wysychania filmu cieczowego na ściance wraz z jego modyfikacją w oparciu o model dwurównaniowy zaprezentowany przez Sedlera i Mikielewicza (1981). Model ten opiera się na bilansie cieczy w rdzeniu przepływu i w filmie cieczowym, gdzie głównym...
-
TQM tools in crisis management
PublikacjaThe current article is concerned with basic aspects connected with improvement of crisis management activities employed by economic operators. The work presents the crisis management model with its fundamental phases and identifies TQM tools that might be applied in all of these phases. The analysis included in the work has been supported with research conducted on companies located in northern Poland
-
Una crisi diversa?
PublikacjaIl capitolo tratta della recente crisi finanziaria, analizzandone cause e conseguenze per il sistema bancario e finanziario mondiale
-
Headspace liquid phase microextraction for quantitation of hexanal in potato crisps by gas chromatography
Publikacja -
Case Studies in Thermal Engineering
Czasopisma -
Case Studies in Construction Materials
Czasopisma -
Case Study and Case Report
Czasopisma -
Business Risk Management in Times of Crises and Pandemics
Publikacja -
Assessment of port facilities security in crisis management
PublikacjaZ punktu widzenia transportu międzynarodowego oraz przemysłowego charakteru systemu portowego, bezpieczeństwo obiektów portowych stanowi ważny element zarówno w zarządzaniu strategicznym portów morskich, jak również element ogólnej koncepcji zarządzania kryzysowego w ujęciu lokalnym, regionalnym, krajowym i międzynarodowym. W celu zapewnienia bezpieczeństwa portów morskich, muszą być prowadzone działania w celu przygotowania administracji...
-
CRISIS MANAGEMENT PLANS IN CONTEXT OF CONTEMPORARY HAZARDS
Publikacja -
Digital Innovations for Sustainable Development in the Time of Crisis
Publikacja -
The trajectories of the financial crisis of companies at risk of bankruptcy
PublikacjaThis article concerns the assessment of the trajectory of the collapse of enterprises in Central Europe. The author has developed a model of a Kohonen artificial neural network. This model was used to determine 6 different classes of risk and was allowed to graphically determine the 5- to 10-year trajectory of going bankrupt. The study used data on 140 companies listed on the Warsaw Stock Exchange. This population was divided into...
-
The Crisis of Marketing Communication in the Era of Social Media
PublikacjaThe articles examines the widely discussed in literature the problem of marketing communication crisis resulting from the changes of the environment. These changes generate both new problems and also new solutions, e.g. social media. The practicioners are pursuing both effective and efficient solutions, in relation to the difficulties encountered while achieving market objectives via traditional methods and channels. In accordance...
-
Journal of Contingencies and Crisis Management
Czasopisma -
Knowledge Management for Managing Crisis in Tourism: Theoretical insights
PublikacjaCOVID-19 has caused an unprecedented crisis affecting the entire tourism industry. Accommodation, food, and travel sectors have been affected by the decreased number of visitors, which had a ripple effect on the financial situation of the whole industry. The purpose of this work is a better understanding of the role of knowledge management in terms of mitigating the crisis in the tourism sector, as well as a development of the...
-
Neurodiversity and remote work in times of crisis: lessons for HR
PublikacjaPurpose The rich qualitative study builds on 11 semi-structured interviews with nine neurodivergent employees and two business professionals supportive of neurodiversity to understand the lived experiences of dealing with crisis in a remote working environment. Design/methodology/approach The purpose of the reported research is to understand how neurominorities experience remote working in the times of crisis and what the implications...
-
Stock Exchanges Indices and Abnormal Returns in the Crisis Condition
Publikacja -
CASE Journal
Czasopisma -
Katarzyna Wojan dr hab.
OsobyDoktor habilitowany nauk humanistycznych w zakresie językoznawstwa; rusycystka i fennistka; profesor uczelni w Instytucie Skandynawistyki i Fennistyki Uniwersytetu Gdańskiego; w latach 2016–2019 pełniła funkcję kierownika Pracowni Języka, Kultury i Gospodarki Finlandii; założycielka i kierownik Pracowni Leksykograficzno-Bibliograficznej oraz Pracowni Języka Fińskiego na Wydziale Filologicznym UG. Autorka i współautorka ponad 200...
-
Economic Crisis, Trust and Socio-Economic Aspects of Sustainable Development
PublikacjaW artykule podjęto tematykę relacji pomiędzy kryzysem ekonomicznym i kryzysem zaufania. Omówiono elementy kreujące i destruktywne w procesie budowy zaufania w gospodarce. Przedstawiono konsekwencje wynikające z zachowań uczestników procesów ekonomicznych oraz relacje zachodzące pomiędzy statusem materialnym inwestorów, a akceptowaniem przez nich wyższego poziomu ryzyka i spadkiem zaufania do systemu ekonomicznego. Wskazano istotność...
-
Trust case - a case for trustworthiness of IT infrastructures
PublikacjaArtykuł prezentuje podejście do budowy argumentów (trust case) uzasadniających zaufanie do systemów i infrastruktur IT. Wprowadza system pojęć związanych ze strukturą i treścią trust case, identyfikuje udziałowców trust case oraz dyskutuje możliwość wsparcia narzędziowego procesu zarządzanie trust case. Omawia równiez krótko dotychczasowe zastosowania tego podejścia.
-
Supporting compliance with safety standards by trust case templates
PublikacjaStandard Compliance (SC) Framework presented in this paper encompasses methods and tools which provide support for application of standards. The framework is based on trust case methodology. A trust case is a data structure which represents a justification that an object (a system, an infrastructure, an organization) exhibits certain properties. It contains an argument and related evidence which support claimed properties. A trust...
-
Risk Hazards & Crisis in Public Policy
Czasopisma -
How can systems thinking help us in the COVID‐19 crisis?
PublikacjaThe COVID-19 pandemic outbreak remains one of the most influential events in the global economy over the recent years. While being primarily public health related, it has a tremendous impact on many other aspects, including business management. Many businesses were forced to introduce rapid changes to their business models in order to survive. The aim of this paper is to show the complexity and interrelations of changes triggered...
-
Forest clear-cuts as additional habitat for breeding farmland birds in crisis
Publikacja -
Poland’s polar policy in the face of the Arctic Council 2022 cooperation crisis
Publikacja -
EU's Energy Policy and Assessing Europe's Spiraling Energy Security Crises
Publikacja -
Possible uses of crisis situation aiding system in virtual world simulation
PublikacjaMany of the real world crisis situations like spreading fire, hostile units attack, flood, and etc. are commonly used in computer games where a simulation of extensive virtual world is crucial. This paper presents some ideas for possible uses of existing crisis situation aiding system in such environments. Moreover, it shows how this kind of system can be taught during subsequent games with a large number of players. As an example...
-
Intercomparison on measurements of PCBs in pork fat during the Belgian PCB-crisis
PublikacjaW pracy przedstawiono wyniki porównania międzylaboratoryjnego oznaczania PCB w tłuszczu wieprzowym. Porównanie międzylaboratoryjne przeprowadzono w związku z tzw. "belgijskim kryzysem PCB" w latach 1999-2000. W porównaniu uczestniczyło 31 laboratoriów. Materiałem do badań były wyprodukowane w Instytucie Metod i Pomiarów Odniesienia (IRMM) w Geel - Belgia, trzy materiały odniesienia zawierające PCB w tłuszczu wieprzowym (suma PCB...
-
Possible uses of crisis situation aiding system in virtual world simulation
PublikacjaMany of the real world crisis situations like spreading fire, hostile units attack, flood, and etc. are commonly used in computer games where a simulation of extensive virtual world is crucial. This paper presents some ideas for possible uses of existing crisis situation aiding system in such environments. Moreover it shows how this kind of system can be taught during subsequent games with a large number of players.
-
Managing Unemployment under COVID-19 Conditions (States of Emergency or Crisis)
PublikacjaRising unemployment is one of the consequences of the COVID-19 pandemic in many countries. This, in turn, has forcedpolicymakers to respond immediately with policy tools to minimize unemployment. The purpose of our study is to contribute toempirical knowledge by looking at activities of 40 local government units to counteract unemployment in the cross-border regionon the Polish side. In doing this, our study contributes to the...
-
Methods of Artificial Intelligence for Prediction and Prevention Crisis Situations in Banking Systems
PublikacjaIn this paper, a support vector machine has been studied due to prediction of bank crisis. To prevent outcomes of crisis situations, artificial neural networks have been characterized as applied to stock market investments, as well as to test the credibility of the bank's customers. Finally, some numerical experiments have been presented.
-
State Interventionism in Tax System - Example of Action in a COVID-19 Crisis
PublikacjaThe following article presents and classifies changes in tax systems of selected countries as well as counts them as the response to the emergence of the crisis caused by the COVID-19 pandemic. Such actions were undertaken the context of state interventionism in the tax system. In order to achieve the objective of presenting the state interventionism in the context of tax rates, an analysis of the literature on the phenomenon described,...
-
CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin
PublikacjaOverexpression of proteins/antigens and other gene-related sequences in the bodies could lead to significant mutations and refractory diseases. Detection and identification of assorted trace concentrations of such proteins/antigens and/or gene-related sequences remain challenging, affecting different pathogens and making viruses stronger. Correspondingly, coronavirus (SARS-CoV-2) mutations/alterations and spread could lead to overexpression...
-
Design of Inner Gate for CRIST Shipyard Dry Dock
PublikacjaThe paper deals with a removable steel inner gate which was designed to separate two parts of a dry dock of about 70 m in width and 380 m in length. The gate allows for independent assembly of ship structures in two separated parts of the dock. The fore part of the dock can be flooded while the after part is dry. Tthe gate was designed by IDEK Company Ltd in 2011 and it was soon constructed and used by CRIST Shipyard in Gdynia.
-
A & A Practice
Czasopisma -
Rapid and sensitive determination of acrylamide in potato crisps using reversed-phase direct immersion single drop microextraction-gas chromatography
Publikacja -
How can Systems Thinking Help Us Handling the COVID-19 Crisis?
PublikacjaPurpose: COVID-19 pandemic outbreak remains one of the most influential events in the global economy over the recent years. While being primarily public health-related, it has a tremendous impact on many other aspects, such as public transport, education, and business management. Many businesses were forced to introduce rapid changes to their business models in order to survive. The aim of this paper is to show the complexity and...