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Search results for: bacteria
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Insights into the microbial community of treated wastewater, its year-round variability and impact on the receiver, using cultivation, microscopy and amplicon-based methods
PublicationApart from chemical constituents, wastewater treatment plant (WWTP) effluents also release microorganisms that can be important to the receiving water bodies either from a sanitary point of view, or taking to the account the biogeochemical potential of the recipients. However, little is known about the treated wastewater microbial community, its composition, seasonal changes, functions and fate in the waters of the receiver. Thus,...
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Tetracycline and ampicillin resistance of Escherichia coli strains of surface water origin: the potential for horizontal transfer of resistance genes = Oporność na tetracyklinę i ampicylinę szczepów Escherichia Coli wyizolowanych z wody powierzchniowej : możliwość horyzontalnego przenoszenia genów oporności
PublicationThe aim of this preliminary study was to assess the occurrence and molecular diversity of tetracycline and ampicillin resistance genes carried by Escherichia coli present in surface water. Bacterial strains were isolated from two watercourses (the Reda River and the Oliwski Stream) that influence the quality of coastal waters in the Gdańsk Bay (Northern Poland) by direct discharge. The bacterial drug susceptibility, tested against...
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Glucosamine-6-phosphate synthase with an oligoHis insert
PublicationGlucosamine-6-phosphate (GlcN-6-P) synthase known also as L-Glutamine: D-fructose-6-phosphate aminotransferase (EC 2.6.1.16), catalyzes the first committed step in the amino sugar biosynthetic pathway in prokaryotic and eukaryotic organisms. The final product of this pathway is an activated precursor of numerous macromolecules containing amino sugars, including chitin and mannproteins in fungi, peptydoglican and lipopolysaccharides...
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Nitrogen removal via the nitrite pathway during wastewater co-treatment with ammonia-rich landfill leachates in a sequencing batch reactor
PublicationThe biological treatment of ammonia-rich landfill leachates due to an inadequate C to N ratio requires expensive supplementation of carbon from an external carbon source. In an effort to reduce treatment costs, the objective of the study was to determine the feasibility of nitrogen removal via the nitrite pathway during landfill leachate co-treatment with municipal wastewater. Initially, the laboratory-scale sequencing batch reactor...
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Diversity and distribution of Tardigrada in Arctic cryoconite holes
PublicationDespite the fact that glaciers and ice sheets have been monitored for more than a century, knowledge on the glacial biota remains poor. Cryoconite holes are water-filled reservoirs on a glacier’s surface and one of the most extreme ecosystems for microinvertebrates. Tardigrada, also known as water bears, are a common inhabitant of cryoconite holes. In this paper we present novel data on the morphology, diversity, distribution and...
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Molecular identification and genotyping of Staphylococci: genus, species, strains, colnes, lineages, and interspecies exchanges
PublicationStaphylococci are increasingly recognized as etiological agents of many opportunistic human and animal infections, emphasizing the need for a rapid and accurate identification, even to a genotypical level of these bacteria. In the recent years, there has been a significant progress in typing and phylogenetic study of Staphylococcus species. Here, we describe molecular methods used in taxonomy as well as staphylococci characterization....
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B-GALACTOSIDASE ARTHROBACTER SP. 32cB - OBTAINING THE GENE SEQUENCE, CONSTRUCTION OF THE EXPRESSION SYSTEM, BIOSYNTHESIS AND BIOCHEMICAL CHARACTERIZATION OF THE ENZYME
PublicationINTRODUCTION: β-Galactosidase is an enzyme which catalyzes the hydrolysis of O glycosidic bond in β-galactosides. Another activity of β galactosidase is a transglycosylation activity. The main industrial use of this protein is the hydrolysis of lactose in milk in a cooling conditions. Synthesis of galactooligosaccharides, which are mostly used as a prebiotics added to some foods or available as dietary supplements, is only one...
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Desensitization of glucosamine-6-phosphate synthase to inhibition by UDP-GlcNAc
PublicationGlucosamine-6-phosphate (GlcN-6-P) synthase, known also as L-Glutamine: D-fructose-6-phosphate amidotransferase, catalyzes the first committed step in the pathway leading to the ultimate formation of UDP-GlcNAc. The final product of this pathway is an activated precursor of numerous macromolecules containing amino sugars, including chitin and mannproteins in fungi, peptidoglycan and lipopolysaccharides in bacteria, and glycoproteins...
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Electrode-based floating treatment wetlands: Insights into design operation factors influencing bioenergy generation and treatment performance
PublicationExponential increases in energy consumption and wastewater have often irreversible environmental impacts. As a result, bio-electrochemical devices like microbial fuel cells (MFCs), which convert chemical energy in organic matter to electricity using exoelectrogenic bacteria, have gained interest. However, operational factors affecting efficiency and energy output need further study. This research investigated bioenergy production...
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Diving boldly into COVID‐19 contaminated wastewater: Eyes at nanotechnology‐assisted solutions
PublicationSeveral studies have been directed to find scalable, swift, accurate, and cost-effective strategies for detecting, monitoring, and treating coronavirus disease 2019 (COVID-19). Indeed, the lack of a fast and practical method for detecting the infected regions makes decision-making challenging to combat the critical pandemic-struck situations. The probable ‘wrong’, or rather inadequate, decisions not only have a boomerang effect...
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Jacek Namieśnik prof. dr hab. inż.
PeopleScientific discipline: chemistryRector in 2016-2019 He was born on 10 December, 1949 in Mogilno. He graduated in 1972 at the Faculty of Chemistry at Gdańsk University of Technology, obtaining a master's degree in chemical engineering. In 1972 he started working at Gdańsk University of Technology, where in 1978 he defended his doctoral thesis and in 1985 he completed his habilitation. He was appointed an associate professor in 1991...
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The microbial community, its biochemical potential, and the antimicrobial resistance of Enterococcus spp. in Arctic lakes under natural and anthropogenic impact (West Spitsbergen)
PublicationThe sustainable management of small human communities in the Arctic is challenging. In this study, both a water supply system (Lake 1) under the natural impact of a bird-nesting area, and a wastewater receiver (Lake 2) were analysed in the vicinity of the Polish Polar Station on West Spitsbergen. Microbial community composition, abundance and activity were assessed in samples of the treated wastewater, lake water and sediments...
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Comparing the permeability of human and porcine small intestinal mucus for particle transport studies
PublicationThe gastrointestinal mucus layer represents the last barrier between ingested food or orally administered pharmaceuticals and the mucosal epithelium. This complex gel structure plays an important role in the process of small intestinal absorption. It provides protection against hazardous particles such as bacteria but allows the passage of nutrients and drug molecules towards the intestinal epithelium. In scientific research, mucus...
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Antibiotic resistance, virulence, and phylogenetic analysis of Escherichia coli strains isolated from free-living birds in human habitats
PublicationWild birds can be colonized by bacteria, which are often resistant to antibiotics and have various virulence profiles. The aim of this study was to analyze antibiotic resistance mechanisms and virulence profiles in relation to the phylogenetic group of E. coli strains that were isolated from the GI tract of wildfowl. Out of 241 faecal samples, presence of E. coli resistant to a cephalosporin (ESBL/AmpC) was estimated for 33 isolates...
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Possibility of Using Tea Fungus for Fermentation of Beetroot and Carrot Pomace Beverages
PublicationFermented beverages obtained from plant-based raw materials are becoming more popular due to their beneficial effects on human health. These include kombucha beverages, which are obtained by fermenting tea brew using the so-called tea fungus, which includes acetic acid bacteria and osmophilic yeast. A consortium of these microorganisms could also be used to prepare functional fermented beverages obtained from extracts of vegetable...
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Antimicrobial resistance of fecal indicators in muncipal wastewater treatment plant
PublicationAntimicrobial resistance of fecal coliforms (n = 153) and enterococci (n = 199) isolates was investigated in municipal wastewater treatment plant (WWTP) based on activated sludge system. The number of fecal indicators (in influent and effluent as well as in the aeration chamber and in return activated sludge mixture) was determined using selective media. Susceptibility of selected strains was tested against 19 (aminoglycosides,...
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Photosensitization of TiO2 and SnO2 by Artificial Self-Assembling Mimics of the Natural Chlorosomal Bacteriochlorophylls
PublicationOf all known photosynthetic organisms, the green sulfur bacteria are able to survive under the lowest illumination conditions due to highly efficient photon management and exciton transport enabled by their special organelles, the chlorosomes, which consist mainly of self-assembled bacteriochlorophyll c, d, or e molecules. A challenging task is to mimic the principle of self-assembling chromophores in artificial light-harvesting...
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Modeling external carbon addition in combined N-P activated sludge systems with an extension of the IWA Activated Sludge Models
PublicationThe aim of this study was to expand the IWA Activated Sludge Model No. 2d (ASM2d) to account for a newly defined readily biodegradable substrate that can be consumed by polyphosphate accumulating organisms (PAOs) under anoxic and aerobic conditions, but not under anaerobic conditions. The model change was to add a new substrate component and process terms for its use by PAOs and other heterotrophic bacteria under anoxic and aerobic...
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Modeling external carbon addition in combined N-P activated sludge systems with an extension of the IWA Activated Sludge Models
PublicationThe aim of this study was to expand the IWA Activated Sludge Model No. 2d (ASM2d) to account for a newly defined readily biodegradable substrate that can be consumed by polyphosphate accumulating organisms (PAOs) under anoxic and aerobic conditions, but not under anaerobic conditions. The model change was to add a new substrate component and process terms for its use by PAOs and other heterotrophic bacteria under anoxic and aerobic...
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Removal of nitrogen from wastewater: Unsolved problems and possible solutions with partial denitrification/anammox systems
PublicationAbstract Nitrate (NO3−) is more stable than ammonia (NH4+) and nitrite (NO2−) among the majority of emerging pollutants in wastewater that can harm the ecosystem by depleting oxygen and water quality, thereby posing environmental and human health risks. Despite anaerobic ammonium oxidation (anammox) being a common method for nitrogen (N) removal from wastewater, it leaves behind residual NO3− of around 11 %. Maintaining the stability...
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A novel approach to enhance high optically active L-lactate production from food waste by landfill leachate
PublicationThe recycling of food waste (FW) through anaerobic fermentation into lactic acid (LA), with two isomers L-LA and D-LA, aligns with the principles of a bio-based circular economy. However, FW fermentation is often limited by competing pathways, acidification inhibition, and trace metals deficiency. This study investigates the introduction of landfill leachate, containing buffering agents (ammonia) and trace metals, into FW fermentation....
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A Perspective on Missing Aspects in Ongoing Purification Research towards Melissa officinalis
PublicationMelissa officinalis L. is a medicinal plant used worldwide for ethno-medical purposes. Today, it is grown everywhere; while it is known to originate from Southern Europe, it is now found around the world, from North America to New Zealand. The biological properties of this medicinal plant are mainly related to its high content of phytochemical (bioactive) compounds, such as flavonoids, polyphenolic compounds, aldehydes, glycosides...
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Electrophoretic Deposition of Chitosan/Eudragit E 100/AgNPs Coatings for Controlled Release of Antibacterial Substance
PublicationThe development of bacterial infection of the tissues surrounding an implant is one of the leading causes of implant surgery failure. In order to prevent the deposition of bacteria on the implant surface and the formation of biofilm, coatings that exhibit antibacterial properties are manufactured. However, the problem is the controlled release of the therapeutic substance from the coating over the extended life of the implant [1],...
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SimLE Stardust: How an experiment evolved from student tinkering to a stratospheric research platform
PublicationStratospheric balloons are one of the most accessible methods of near space exploration. Their applications include capturing of extreme living organisms whose existence in the stratosphere has been suggested in the literature. These potential microorganisms would have evolved to survive the unique environment of low temperature, low pressure and high solar radiation. This makes the development of...
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Fungal Typin Methods
PublicationThe broad application of the molecular techniques in mycoses diagnosis is related to increase of infections caused by fungi in many countries. The oldest typing methods relaying on fenotypic observation, physiological and biochemical examination have had very limited importance from decades. Novadays, the molecular biology methods took their place. Most of the genotyping methods have been devoleped to be applied for typing of bacteria...
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MutS3: a MutS homologue of unknown biological function
PublicationThe homologues of MutS proteins are widespread among both Prokaryotes and Eukaryotes. MutS designated as MutS1 is a part of MMR (mismatch repair) system which is responsible for removal of mispaired bases and small insertion/deletion loops in DNA. Initially, the only MutS homologues known were those engaged in mismatch repair and these were later designated as MutS1. Subsequently, the MutS2 homologue was distinguished. MutS2 does...
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Ultrafiltration Process in Disinfection and Advanced Treatment of Tertiary Treated Wastewater
PublicationThe paper presents the results of research on the use of ultrafiltration, using membranes of 200 and 400 kDa separation, for disinfection of municipal treated wastewater. The research was conducted on a fractional technical scale using real municipal treated wastewater from two large wastewater treatment plants treating most of the wastewater over the one-million polycentric Gdańsk agglomeration (1.2 million inhabitants). UF 200...
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Molecular transformation of dissolved organic matter in manganese ore-mediated constructed wetlands for fresh leachate treatment
PublicationThe organic matter (OM) and nitrogen in Fresh leachate (FL) from waste compression sites pose environmental and health risks. Even though the constructed wetland (CW) can efficiently remove these pollutants, the molecular-level transformations of dissolved OM (DOM) in FL remain uncertain. This study reports the molecular dynamics of DOM and nitrogen removal during FL treatment in CWs. Two lab-scale vertical-flow CW systems were...
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Direct injection liquid chromatography-mass spectrometry (DI-LC-MS) analysis for rapid lipidomic profiling of extracellular vesicles
PublicationExtracellular vesicles (EVs) are small, spherical particles produced by eukaryotic and prokaryotic cells, surrounded by a bilayer membrane and carrying various bioactive molecules, such as proteins, surface receptors, membrane and soluble proteins, lipids, and nucleic acids. EVs are of substantial interest because of their important roles in cell communication, epigenetic regulation and possible application in disease diagnosis...
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The mechanical properties and bactericidal degradation effectiveness of tannic acid-based thin films for wound care
PublicationThe surface area is the most important aspect when considering the interactions between a material and the surrounding environment. Chitosan (CTS) and tannic acid (TA) were previously successfully tested by us to obtain thin films to serve as wound dressings or food packaging materials. However, surface properties as well as the antimicrobial activity of the material were not considered. They are important if the material is likely...
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Modelling of dark fermentation of glucose and sour cabbage
PublicationIn the article, modified Anaerobic Digestion Models 1 (ADM-1) was tested for modelling dark fermentation for hydrogen production. The model refitting was done with the Euler method. The new model was based on sets of differential equations. The model was checked for hydrogen production from sour cabbage in batch and semi-batch in 5 g VSS (volatile solid suspension)/L and at the semi-batch process from glucose at 5 and 10 g VSS/L....
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The influence of biological environment on the silver-coated implants
PublicationThis paper deals with implants covered with nanosilver
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Bacteriological monitoring of coastal sea waters – Gulf of Gdańsk (Baltic Sea) in 2009
Open Research DataThe dataset contains the results of a bacteriological and physicochemical studies of Gdansk Bay coastal waters within the administrative boundaries of Gdańsk city in 2009. The examined material consisted of water samples collected from 15 sampling points. The aim of this study was to determine the number of Escherichia coli and the number of intestinal...
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The results of physico – chemical and microbiological testing of watercourses around the municipality of Gdańsk in 2009
Open Research DataThe dataset contains the results of a bacteriological and physico-chemical study of watercourses waters within the administrative boundaries of Gdańsk city in 2009. The examined material consisted of water samples collected from 16 watercourses within the administrative boundaries of Gdańsk, Poland.
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The results of physico – chemical and microbiological testing of watercourses around the municipality of Gdańsk in 2011
Open Research DataThe dataset contains the results of a bacteriological and physico-chemical study of watercourses waters within the administrative boundaries of Gdańsk city in 2011. The examined material consisted of water samples collected from 16 watercourses within the administrative boundaries of Gdańsk, Poland.
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The results of physico – chemical and microbiological testing of watercourses around the municipality of Gdańsk in 2009
Open Research DataThe dataset contains the results of a bacteriological and physico-chemical study of watercourses waters within the administrative boundaries of Gdańsk city in 2009. The examined material consisted of water samples collected from 16 watercourses within the administrative boundaries of Gdańsk, Poland.
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Bacteriological and physicochemical monitoring of inland surface waters within the administrative boundaries of Gdańsk city in 2010
Open Research DataThe dataset containst the results of a bacteriological and physico-chemical study of inland surface waters within the administrative boundaries of Gdańsk city in 2010.The examined material consisted of water samples collected from four lakes located within the administrative boundaries of Gdańsk, Poland.
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Bacteriological monitoring of coastal sea waters – Gulf of Gdańsk (Baltic Sea) in 2010
Open Research DataThe dataset contains the results of a bacteriological and physicochemical studies of Gdansk Bay coastal waters within the administrative boundaries of Gdańsk city in 2010. The examined material consisted of water samples collected from 15 sampling points. The aim of this study was to determine the number of Escherichia coli and the number of intestinal...
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The results of physico – chemical and microbiological testing of watercourses around the municipality of Gdańsk in 2010
Open Research DataThe dataset contains the results of a bacteriological and physico-chemical study of watercourses waters within the administrative boundaries of Gdańsk city in 2010. The examined material consisted of water samples collected from 16 watercourses within the administrative boundaries of Gdańsk, Poland.
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Bacteriological and physicochemical monitoring of inland surface waters within the administrative boundaries of Gdańsk City in 2008
Open Research DataThe dataset containst the results of a bacteriological and physico-chemical study of inland surface waters within the administrative boundaries of Gdańsk city in 2008. The examined material consisted of water samples collected from four lakes located within the administrative boundaries of Gdańsk, Poland.
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Bacteriological and physicochemical monitoring of inland surface waters within the administrative boundaries of Gdańsk City in 2009
Open Research DataThe dataset containst the results of a bacteriological and physico-chemical study of inland surface waters within the administrative boundaries of Gdańsk city in 2009. The examined material consisted of water samples collected from four lakes located within the administrative boundaries of Gdańsk, Poland.
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Bacteriological and physicochemical monitoring of inland surface waters within the administrative boundaries of Gdańsk City in 2011
Open Research DataThe dataset containst the results of a bacteriological and physico-chemical study of inland surface waters within the administrative boundaries of Gdańsk city in 2011. The examined material consisted of water samples collected from four lakes located within the administrative boundaries of Gdańsk, Poland.
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Microbiological condition of sediments and bottom water in the area of Gdańsk Deep in Gulf of Gdańsk
Open Research DataThis dataset contains the results of microbiological analysis of bottom water and bottom sediments in the area of Gdańsk Deep in Gulf of Gdańsk. The tested samples were collected at 5 sites on 15th of December 2007. 5 samples of bottom water and 10 samples of sediments were collected for microbiological testing. Each of these samples were analysed for...
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Bacteriological monitoring of coastal sea waters – Gulf of Gdańsk (Baltic Sea) in 2008
Open Research DataThe dataset contains the results of a bacteriological and physicochemical studies of Gdansk Bay coastal waters within the administrative boundaries of Gdańsk city in 2008. The examined material consisted of water samples collected from 15 sampling points. The aim of this study was to determine the number of Escherichia coli and the number of intestinal...
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The results of physico – chemical and microbiological testing of watercourses around the municipality of Gdańsk in 2005
Open Research DataThe dataset contains the results of a bacteriological and physico-chemical study of watercourses waters within the administrative boundaries of Gdańsk city in 2005. The examined material consisted of water samples collected from 15 watercourses within the administrative boundaries of Gdańsk, Poland.
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Bacteriological and physicochemical monitoring of coastal sea waters – Gulf of Gdańsk (Baltic Sea) in 2004
Open Research DataThe dataset contains the results of a bacteriological and physicochemical studies of Gdansk Bay coastal waters within the administrative boundaries of Gdańsk city in 2004. The examined material consisted of water samples collected from 15 sampling points. Number of Escherichia coli, number of intestinal enterococci, presence of Salmonella sp., pH, temperature,...
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The results of physico – chemical and microbiological testing of watercourses around the municipality of Gdańsk in 2004
Open Research DataThe dataset contains the results of a bacteriological and physico-chemical study of watercourses waters within the administrative boundaries of Gdańsk city in 2004. The examined material consisted of water samples collected from 15 watercourses within the administrative boundaries of Gdańsk, Poland.
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The results of physico – chemical and microbiological testing of watercourses around the municipality of Gdańsk in 2003
Open Research DataThe dataset contains the results of a bacteriological and physico-chemical study of watercourses waters within the administrative boundaries of Gdańsk city in 2003. The examined material consisted of water samples collected from 14 watercourses within the administrative boundaries of Gdańsk, Poland.
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Bacteriological and physicochemical monitoring of coastal sea waters – Gulf of Gdańsk (Baltic Sea) in 2006
Open Research DataThe dataset contains the results of a bacteriological and physicochemical studies of Gdansk Bay coastal waters within the administrative boundaries of Gdańsk city in 2006. The examined material consisted of water samples collected from 15 sampling points. Number of Escherichia coli, number of intestinal enterococci, presence of Salmonella sp., pH, temperature,...
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Bacteriological and physicochemical monitoring of inland surface waters within the administrative boundaries of Gdańsk city in 2006
Open Research DataThe dataset containst the results of a bacteriological and physico-chemical study of inland surface waters within the administrative boundaries of Gdańsk city in 2006.