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Search results for: SPECIES IDENTIFICATION TESTS
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A Computational Analysis of the Proton Affinity and the Hydration of TEMPO and Its Piperidine Analogs
PublicationThe study investigated the impact of protonation and hydration on the geometry of nitroxide radicals using B3LYP and M06-2X methods. Results indicated that TEMPO exhibited the highest proton affinity in comparison to TEMPOL and TEMPONE. Two pathways contribute to hydrated protonated molecules. TEMPO shows lower first enthalpies of hydration (ΔH1-M), indicating stronger H-bonding interactions, while TEMPONE shows higher values,...
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Efficient production of Staphylococcus simulans lysostaphin in a benchtop bioreactor by recombinant Escherichia coli.
PublicationLysostaphin is an enzyme with bactericidal activity against Staphylococcus aureus and other staphylococcal species. In spite of many advantages and promising results of preliminary research, the enzyme is still not widely used in medicine, veterinary or as a food preservative. One of the most important factors limiting application of the enzyme in clinical or technological practice is the high costs of its production. In the present...
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Molecular mechanism of proton-coupled ligand translocation by the bacterial efflux pump EmrE
PublicationThe current surge in bacterial multi-drug resistance (MDR) is one of the largest challenges to public health, threatening to render ineffective many therapies we rely on for treatment of serious infections. Understanding different factors that contribute to MDR is hence crucial from the global “one health” perspective. In this contribution, we focus on the prototypical broad-selectivity proton-coupled antiporter EmrE, one of the...
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A New Expression System Based on Psychrotolerant Debaryomyces macquariensis Yeast and Its Application to the Production of Cold-Active β-D-Galactosidase from Paracoccus sp. 32d
PublicationYeasts provide attractive host/vector systems for heterologous gene expression. The currently used yeast-based expression platforms include mesophilic and thermotolerant species. A eukaryotic expression system working at low temperatures could be particularly useful for the production of thermolabile proteins and proteins that tend to form insoluble aggregates. For this purpose, an expression system based on an Antarctic psychrotolerant...
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Analysis of volatile fraction selected hybrid fruits using chromatographic techniques
PublicationAt present, "healthy eating" is gaining in popularity. Many people associate this term with eating plenty of vegetables and fruits. The basic elements influencing the selection of fruit are their appearance and smell. Of course, their origin and impact on human health are also very important. In recent years, exotic fruits have become increasingly accessible, making them gaining popularity, even among Poles. You can also meet plants...
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Imidazoacridinone-dependent lysosomal photodestruction: a pharmacological Trojan horse approach to eradicate multidrug-resistant cancers
PublicationMultidrug resistance (MDR) remains a primary hindrance to curative cancer therapy. Thus, introduction of novel strategies to overcome MDR is of paramount therapeutic significance. Sequestration of chemotherapeutics in lysosomes is an established mechanism of drug resistance. Here, we show that MDR cells display a marked increase in lysosome number. We further demonstrate that imidazoacridinones (IAs), which are cytotoxic fluorochromes,...
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Molecular taxonomy of scopulariopsis-like fungi with description of new clinical and environmental species
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Dermatophyte species in superficial mycoses in the Kraków district, Poland in the years 1972-2007
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The effect of electromagnetic field on reactive oxygen species production in human neutrophilsin vitro
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A strongly bound OF3- anion and its unstable parent neutral OF3 species
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INDIVIDUAL TREE DETECTION FROM UAV LIDAR DATA IN A MIXED SPECIES WOODLAND
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Habitat displacement effect between two competing owl species in fragmented forests
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Biologically Active Preparations from the Leaves of Wild Plant Species of the Genus Rubus
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Phytochemical Profiling of Extracts from Rare Potentilla Species and Evaluation of Their Anticancer Potential
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Description of plants and assessment of chemical properties of three species from the Amelanchier genus
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Variation in Total Polyphenolics Contents of Aerial Parts of Potentilla Species and Their Anticariogenic Activity
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New species and records ofLepraria(Stereocaulaceae, lichenized Ascomycota) from South America
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Coprinopsis rugosomagnispora: a distinct new coprinoid species from Poland (Central Europe)
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MOLECULAR AND QUALITY MEAT CHARACTERISTICS OF NEW FISH SPECIES INTRODUCED TO THE EU MARKET
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Semi-industrial development of nutritious and healthy seafood dishes from sustainable species
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Phototransformation of three selected pharmaceuticals, naproxen, 17α-Ethinylestradiol and tetracycline in water: Identification of photoproducts and transformation pathways
PublicationTransformation products (TPs) may be formed during various processes, however, it was proved that one of the main mechanisms responsible for degradation of pharmaceuticals in natural waters is photolysis. Three compounds were selected, tetracycline (TC), 17-α-ethinylestradiol (EE2) and naproxen (NAP), for degradation experiments which were performed using a xenon lamp emitting light with a spectrum closely approximating that of...
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Results of acoustic emission tests of corrosion protection degree of ship steel tanks covered by means of protective coatings (Phase I)
PublicationThis paper presents methods and results of the 1st series of tests of acoustic emission (AE) signals, aimed at assessing degree of corrosion protection of a tank made of hull structural steel, protected by means of a protective coating combined from polyurethane layer and concrete filler. The tests were carried outin compliance with a method specially elaborated for application to the work in question. In the method were used requirements...
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Mathematical Models of Control Systems of Angular Speed of Steam Turbines for Diagnostic Tests of Automatic and Mechatronic Devices
PublicationAccurate modeling of physical processes of many automatics and mechatronics systems is often necessity. In power system such a process is control of angular velocity of power objects during connection to operation in parallel. This process is extremely dynamic. For this reason response of control system depends from changes of many physical parameters (temperature, pressure and flow of the medium, etc.). Precision modeling influences...
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The use of a one-step PCR method for the identification of Microsporum canis and Trichophyton mentagrophytes infection of pets
PublicationIntroduction: Dermatophytes are a closely related group of keratinophilic fungi. They encompass important etiological agents of superficial fungal infections. These fungi are able to invade keratinized tissues of humans and animals, causing dermatophytosis (ringworm) of hair, nails or skin. The aim: Traditional diagnostics of ringworm is based on morphological identification of cultured fungi and is time-consuming. Materials and methods:...
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Identification of inertia and activity in the market behaviour of enterprises
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Identification of toxic metals in human embryonic tissues
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Advanced Agent Identification With Fluctuation-Enhanced Sensing
PublicationPraca przedstawia wyniki szeregu zaawansowanych algorytmów przetwarzania sygnałów losowych obserwowanych w sensorach gazu typu Taguchi Gas Sensors. Stwierdzono, że proponowane metody pozwalają wykrywać bardziej efektywnie obecność wielu różnych gazów. W celu detekcji zastosowano głównie funkcje spektrum i bispektrum.
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Identification and simulation of shells geometric initial imperfection
PublicationPrzedstawiono stochastyczną analizę stalowych zbiorników walcowych ze wstępnymi imperfekcjami geometrycznymi. Imperfekcje opisano za pomocą jednorodnych pól losowych.
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Identification in a Laboratory Tunnel to Control Fluid Velocity
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Delamination Identification Using Global Convolution Networks
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Wavelet analysis for damage identification in composite structures
Publicationw pracy zaproponowano metodę analizy sygnalu opartą o transformatę falkową, pozwalajacą na identyfikcaje pęknięcia zmęczeniowego w jednowymiarowych elementach konstrukcyjnych wykonanych z materialow kompozytowych
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Identification of nonlinear viscoelastic properties of the ''Panama'' fabric
PublicationTkanina techniczna Panama wytwarzana przez firmę Sattler (Niemcy)wykazuje cechy nieliniowe w tym właściwości lepkosprężyste w czasie testów długoterminowych. Na podstawie jednoosiowych testów płynięcia i regeneracji przeprowadzonych dla pięciu poziomów obciążenia przeprowadzono identyfikacje nieliniowych lepkosprężystych właściwości tkaniny. Do opisu zależności konstytutywnych użyto całkowego modelu Schapery'ego. Zastosowano funkcję...
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Stakeholder identification as a determinant of sustainable project management
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The evidential value of dental calculus in the identification process
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A Statistical Approach to the Identification of Diploid Cellular Automata
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Radar technique in application of interlayer identification connections
PublicationOne of important issues in pavement construction is to ensure that layers applied directly one on top of the other make up a continuous whole and form an integral construction complex. An appropriate interlayer connection plays here significant role ensuring proper transmission of distortions generated by wheels of vehicles to lower layers and preventing water penetration between the layers. In case of damaged pavements where lack...
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Primary role identification in dynamic social networks
PublicationIdentyfikacja ról w sieci społecznej jest jednym z podstawowych zagadnień analizy takich sieci. W artykule przedstawiamy nowe podejście do tego zagadnienia. Pokazujemy w jaki sposób można dokonać identyfikacji ról poprzez wykorzystanie zaproponowanego modelu zachowań aktorów. Model taki tworzą podgrafy wzorcowe oraz diagramy stanów okreslające sekwencje aktywności w zachowaniu aktorów. Na bazie wyznaczonych modeli zachowań oraz...
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Crack identification in cantilever beam using wavelets
PublicationPrzedstawiono metodę identyfikacji zniszczeń przy użyciu transformacji falkowej. Rozpatrzono przykład belki wspornikowej. Pokazano, że transformacja falkowa wykrywa miejsce zniszczenia.
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Modelling and Identification of Tower Type Historic Buildings
PublicationW pracy omawia się problemy modelowania i identyfikacji zabytkowych murowanych budowli wieżowych na przykładzie wieży Twierdzy Wisłoujście. Przedstawiono analizę modalną na podstawie dwukierunkowych pomiarów przyspieszeń na 9 poziomach wieży. Wyniki analizy posłużyły do identyfikacji modelu matematycznego budowli w postaci sztywnego bloku na podłożu sprężystym. Wykorzystano hierarchiczną procedurę opartą na analizie wrażliwości.
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Identification of transition curves in vehicular roads and railways
PublicationIn the paper attention is focused on the necessity to systematize the procedure for determining the shape of transition curves used in vehicular roads and railway routes. There has been presented a universal method of identifying curvature in transition curves by using differential equations. Curvature equations for such known forms of transition curves as clothoid, quartic parabola, the Bloss curve, cosinusoid and sinusoid, have...
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Wavelet Analysis for Damage Identification in Composite Structures
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A method of identification of RTS components in noise signals
PublicationW artykule przedstawiono oryginalną metodę wydzielania szumu RTS (Random Telegraph Signal) - dwupoziomowego lub wielopoziomowego - z sygnału szumowego. Podstawą oceny jakości metody jest założenie, że wartości chwilowe szumu RTS mają rozkład niegaussowski natomiast pozostała część sygnału ma rozkład gaussowski.Algorytm identyfikacji wielopoziomowych szumów RTS w sygnałach szumowych małej częstotliwości oparty został na aproksymacji...
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Identification of safety hazards and their sources in tram transport
PublicationBoth in Poland and in most countries of the European Union, the tram infrastructure is extensively expanding. Contemporary low-floor trams are constructed according to different technical assumptions than typical railway vehicles or high floor trams. In Poland such vehicles rides often on lines designed according to standards developed to the classical vehicles. At the same time, the intensity of road traffic and the number of...
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Geoinformation technologies in the identification of meandering section of the river
PublicationPublikacja traktuje o wykorzystaniu naziemnego skaningu laserowego dla potrzeb analiz przestrzennych w zakresie meandrowania rzeki.
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Optical glyphs based localization and identification system
PublicationThe paper presents a description of functioning of a platform supporting the detection of obstructive diseases in the respiratory system education process. A 16-parameter model of the respiratory system simulated in the MATLAB/Simulink environment was set in the role of the tested patient. It has been linked to the control layer, developed in the LabVIEW environment, using the SIT library (Simulation Interface Toolkit). This layer...
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Post-Chromatographic Derivatization Coupled with Mass Spectrometry as a Method of Profiling and Identification of Antioxidants; Ligustrum vulgare Phytocomplex as an Example
PublicationHigh-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) coupled with radical scavenging assays, such as 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) can be both used for the detection of the antioxidants in plant extracts. In this study, the ethanolic (70% v/v) extracts from different morphological parts of Ligustrum vulgare collected...
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Nanoindentation tests of the hydroxyapatite composite coatings applicated on titanium alloys by the electrophoretic method
Open Research DataCurrently, there are no metal materials that meet all biomechanical and biochemical requirements needed for long life implantable biomaterials. The main purpose of the study was to functionalize the surface of the titanium alloys used in biomaterial implants.
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Identification and characterization of the Indian Yellow dyestuff and its degradation products in historical oil paint tube by liquid chromatography mass spectrometry
PublicationAn analytical protocol for identification of dyes using reversed phase liquid chromatography-mass spectrometry with atmospheric pressure electrospray ionization (LC-ESI/MS) is presented. The developed method has been successfully applied in identification of euxanthic acid and euxanthone, the main components of the Indian Yellow dye in a historical oil paint tube produced by Richard Ain`es, a recognizable French company supplying...
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Characterization of aggregate interlocking in hot mix asphalt by mechanistic performance tests
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The assessment of load-settlement curve for Atlas piles correlated with CPT tests
PublicationRacjonalne stosowanie pali przemieszczeniowych typu Atlas wymaga analitycznej oceny pełnej krzywej obciążenie - osiadanie. W pracy przedstawiono możliwości wykorzystania metody funkcji transformacyjnych t-z (dla pobocznicy) oraz q-z (dla podstawy) dla oceny krzywej osiadania. Jednostkowe graniczne opory na pobocznicy, tmax, oraz pod podstawą, gf, określono na podstawie metody bezpośredniej, wykorzystując opory stożka sondy, qc....