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Search results for: GENOME SIZE
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Prevalence of Closely Related Candida albicans Species among Patients with Vulvovaginal Candidiasis in Southern Poland Based on the hwp1 Gene Amplification
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P.1.a.027 Association between the polymorphism of 5-HTTLPR gene and affective temperament dimensions using the TEMPS-A scale in obesity
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THE GENE CLONING, OVEREXPRESSION, PURIFICATION AND BIOCHEMICAL CHARACTERISATION OF A NEW COLD-ADAPTED β–GALACTOSIDASE FROM ARTHROBACTER SP. VII-4
PublicationINTRODUCTION. β-Galactosidase [EC 3.2.1.23] is an enzyme that catalyzes the hydrolysis of O-glycosidic linkages in galactosides. It is commercially used in dairy industry for the production of milk with reduced lactose content. Potentially, the best method for lactose removal under cooling conditions should be carried out with a cold-adapted enzyme. AIM. The aim of this study was to determine the taxonomic affiliation of the isolate...
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A Study on Emission of Airborne Wear Particles from Car Brake Friction Pairs
PublicationThe emission of airborne wear particles from friction material / cast iron pairs used in car brakes was investigated, paying special attention to the influence of temperature. Five low-metallic materials and one non-asbestos organic material were tested using a pin-on-disc machine. The machine was placed in a sealed chamber to allow airborne particle collection. The concentration and size distribution of 0.0056 to 10 μm particles...
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Knowledge management approaches among KIBS companies and their determinants – case study analysis
PublicationThis paper aims to present knowledge management (KM) approaches manifested by knowledge intensive business service (KIBS) companies together with their potential determinants (company size, type of services offered, and organizational structure). In particular, two types of approaches have been selected and examined, i.e. emergent KM approach and deliberate KM approach. Indeed, although KM approaches have been abundantly investigated...
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An integrated geoarchaeological approach to late Iron Age settlement at Kaštelina hillfort (Lopar, Island of Rab, Croatia) using Amplitude Data Comparison (ADC) method and trial excavation
PublicationGeophysical prospection and small-scale archaeological excavation were undertaken on the site of Kaštelina, a Late Iron Age hillfort settlement located on the Stolac promontory in the northern part of Rab island (Kvarner Gulf, Croatia). Within the frame of the “Archaeological topography of the island of Rab” program, a Polish–Croatian team applied a series of multidisciplinary methods to study the occupational history of the...
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Phytoecdysteroid containing plants – a source of bioactive compounds with potential anticarcinogenic effects
PublicationEcdysteroids are a group of steroids synthesized in inver- tebrates as molting hormones and also in some plants as secondary metabolites acting as a defense against parasites. These compounds do not naturally occur in mammals and are not structurally related to mammalian steroid hor- mones, consequently do not activate mammalian hormone receptors. Ecdysteroids exhibit very low toxicity to mam- mals and to date no serious side effects...
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Can mutations in the gene encoding transcription factor EKLF (Erythroid Krüppel-Like Factor) protect us against infectious and parasitic diseases?
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<b><i>CTLA4</i></b> and <b><i>CD28</i></b> Gene Polymorphisms with Respect to Affective Symptom Domain in Schizophrenia
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Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells
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New Gene Markers of Angiogenesis and Blood Vessels Development in Porcine Ovarian Granulosa Cells during Short-Term Primary Culture In Vitro
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Functional Polymorphism in the Interleukin 6 (IL6) Gene with Respect to Depression Induced in the Course of Interferon-α and Ribavirin Treatment in Chronic Hepatitis Patients
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Construction and Use of a Broad-Host-Range Plasmid Expressing the lamB Gene for Utilization of Bacteriophage λ Vectors in the Marine Bacterium Vibrio harveyi
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A new B-D-galactosidase with a low temperature optimum isolated from the Antarctic Arthrobacter sp. 20B: gene cloning, purification and characterization.
PublicationA psychrotrophic bacterium producing a coldadaptedB-galactosidase upon growth at low temperatureswas classiWed as Arthrobacter sp. 20B. A genomic DNAlibrary of strain 20B introduced into Escherichia coliTOP10F' and screening on X-Gal (5-bromo-4-chloro-3-indolyl-B-D-galactopyranoside)-containing agar plates ledto the isolation of B-galactosidase gene. The B-galactosidasegene (bgaS) encoding a protein of 1,053 amino acids,with a...
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Ambulatory Systolic Blood Pressure is Related to the Deletion Allele of the Angiotensin I Converting Enzyme Gene in Young Normotensives with Parental History of Hypertension
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Complete tumour regressions induced by vaccination with IL-12 gene-transduced tumour cells in combination with IL-15 in a melanoma model in mice
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Determination of BRAF V600E (VE1) protein expression and BRAF gene mutation status in codon 600 in borderline and low-grade ovarian cancers
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Analysis of epigenetic clocks links yoga, sleep, education, reduced meat intake, coffee, and a SOCS2 gene variant to slower epigenetic aging
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A novel cold-active β-D-galactosidase with transglycosylation activity from the Antarctic Arthrobacter sp. 32cB - gene cloning, purification and characterization
PublicationA gene encoding a novel β-D-galactosidase from the psychrotolerant Antarctic bacterium Arthrobacter sp. 32cB was isolated, cloned and expressed in Escherichia coli. The active form of recombinant β-D-galactosidase consists of two subunits with a combined molecular weight of approximately 257 kDa. The enzyme's maximum activity towards o-nitrophenyl-β-D-galactopyranoside was determined as occurring at 28 °C and pH 8.0. However, it...
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Multiple transcriptional factors regulate transcription of the rpoE gene in Escherichia coli under different growth conditions and when the lipopolysaccharide biosynthesis is defective.
PublicationThe RpoE sigma factor is essential for the viability of Escherichia coli. RpoE regulates extracytoplasmic functions including lipopolysaccharide (LPS) translocation and some of its non-stoichiometric modifications. Transcription of the rpoE gene is positively autoregulated by EσE and by unknown mechanisms that control the expression of its distally located promoter(s). Mapping of 5′ ends of rpoE mRNA identified five new transcriptional...
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Untargeted Lipidomics Analysis of the Cyanobacterium Synechocystis sp. PCC 6803: Lipid Composition Variation in Response to Alternative Cultivation Setups and to Gene Deletion
PublicationCyanobacteria play an important role in several ecological environments, and they are widely accepted to be the ancestors of chloroplasts in modern plants and green algae. Cyanobacteria have become attractive models for metabolic engineering, with the goal of exploring them as microbial cell factories. However, the study of cyanobacterial lipids’ composition and variation, and the assessment of the lipids’ functional and structural...
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Identification and properties of the psychrophilic bacterium Desulfolatea psychrophila single-stranded DNA binding proteins
PublicationTo study the biochemical properties of SSB from Desulfolatea psychrophila (DpsSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 426 nucleotides encoding SSB protein of 142 amino acids with a calculated molecular mass of 15.6 kDa and it is the smallest known bacterial SSB protein indentified to date. The amino-acid sequence...
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The Chitosan-Based System with Scutellariae baicalensis radix Extract for the Local Treatment of Vaginal Infections
PublicationScutellarie baicalensis radix, as a flavone-rich source, exhibits antibacterial, antifungal, an-tioxidant, and anti-inflammatory activity. It may be used as a therapeutic agent to treat various
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Cross-border transmission line configuration influence on the electrical power and energy billing process
PublicationIn 2015 a connection between the Polish and Lithuanian power systems become fully operational. The connection consists of a 400 kV double circuit transmission line and a back-to-back HVDC substation located on the Lithuanian side. A magnetic couplings between the circuits of the transmission line cause that during power transmission a different values of active power losses are observed in both circuits. This situation may cause...
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Getting to know the potential of social media in forest education
PublicationThe development of social network sites not only facilitates the acquisition and deepening of knowledge but also provides the possibility of easily contacting foresters, specialists in natural sciences and nature enthusiasts. In addition, for some years...
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Biofilm Production and Presence of ica and bap Genes in Staphylococcus aureus Strains Isolated from Cows with Mastitis in the Eastern Poland
PublicationThe aim of the study was phenotypic and genotypic analysis of 132 S. aureus strains isolated from mastitis in eastern Poland in respect to their biofilm formation ability. The analysis of the size polymorphism of fragment X in the gene encoding protein A (spa) revealed high genetic differentiation of the analyzed group of isolates. The ability of biofilm formation by the isolates was tested using two phenotypic methods. The Congo...
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Personal Branding and Brand Loyalty, Social Network Users Brand Identification: Polish-French Model
PublicationSocial network brand sites are increasingly attracting the attention of scientists and managers intrigued by their potential application for brand value creation. The aim of this research, based on a multinational sample, is to fill the gap in understanding how users choose among social networking sites as an act of brand identification. The authors of the paper point to the fact that creating a personal brand is becoming more...
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Distinctive facades of commercial buildings and the quality of public space,
PublicationMutual relations between the retail center and public spaces of the city are varied. They depend on the type and size of the commercial structure. Other ones are for multifunctional shopping centers and other for high streets. In megastores and shopping malls the essential for their functioning is their interior and entrance, which is sometimes the only place of interpenetration of the commercial structure with the surrounding...
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A study on the interaction of rhodamine B with methylthioadenosine phosphorylase protein sourced from an Antarctic soil metagenomic library.
PublicationThe presented study examines the phenomenon of the fluorescence under UV light excitation (312 nm) of E. coli cells expressing a novel metagenomic-derived putative methylthioadenosine phosphorylase gene, called rsfp, grown on LB agar supplemented with a fluorescent dye rhodamine B. For this purpose, an rsfp gene was cloned and expressed in an LMG194 E. coli strain using an arabinose promoter. The resulting RSFP protein was purified...
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Novel single-stranded DNA-binding protein from psyvhrophilic bacterium Psychrobacter arcticus
PublicationTo study the biochemical properties of SSB from Psychrobacter arcticus (ParSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The gene consists of an open reading frame of 642 nucleotides encoding SSB protein of 213 amino acids with a calculated molecular mass of 22.8 kDa. The amino-acid sequence of ParSSB exhibits 49% identity and 57% similarity to Escherichia coli SSB....
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Resonator-Loaded Waveguide Notch Filters with Broad Tuning Range and Additive-Manufacturing-Based Operating Frequency Adjustment Procedure
PublicationThis article presents a new class of ring-resonator-loaded waveguide notch filters with a broad tuning range, low cost, and improved performance. The proposed approach employs a comple-mentary asymmetric split ring resonator coupled to a microstrip transmission line and excited in a rectangular waveguide. An equivalent circuit model is proposed to explain the working principle of the proposed notch filter. The adjustment of the...
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Relationship between Telomere Length, TERT Genetic Variability and TERT, TP53, SP1, MYC Gene Co-Expression in the Clinicopathological Profile of Breast Cancer
PublicationThe molecular mechanisms of telomerase reverse transcriptase (TERT) upregulation in breast cancer (BC) are complex. We compared genetic variability within TERT and telomere length with the clinical data of patients with BC. Additionally, we assessed the expression of the TERT, MYC, TP53 and SP1 genes in BC patients and in BC organoids (3D cell cultures obtained from breast cancer tissues). We observed the same correlation in the...
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Allelic length of a CA dinucleotide repeat in theegfr gene correlates with the frequency of amplifications of this sequence—first results of an inter-ethnic breast cancer study
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New Gene Markers for Metabolic Processes and Homeostasis in Porcine Buccal Pouch Mucosa during Cells Long Term-Cultivation—A Primary Culture Approach
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Abscisic Acid Regulates the 3-Hydroxy-3-methylglutaryl CoA Reductase Gene Promoter and Ginsenoside Production in Panax quinquefolium Hairy Root Cultures
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Mosaic Structure of p1658/97, a 125-Kilobase Plasmid Harboring an Active Amplicon with the Extended-Spectrum β-Lactamase Gene bla SHV-5
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Modelowanie ciągów danych z użyciem sieci neuronowych
PublicationRozdział opisuje problematykę przetwarzania ciągów danych. Opisane zostały typy ciągów danych: dane sekwencyjne, sekwencje czasowe oraz przebiegi czasowe. Przedstawiona została architektura sieci rekurencyj
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Design of a Shape-Memory-Alloy-Based Carangiform Robotic Fishtail with Improved Forward Thrust
PublicationShape memory alloys (SMAs) have become the most common choice for the development of mini- and micro-type soft bio-inspired robots due to their high power-to-weight ratio, ability to be installed and operated in limited space, silent and vibration-free operation, biocompatibility, and corrosion resistance properties. Moreover, SMA spring-type actuators are used for developing different continuum robots, exhibiting high degrees...
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On the Origin of Protein Superfamilies and Superfolds
PublicationDistributions of protein families and folds in genomes are highly skewed, having a small number of prevalent superfamiles/superfolds and a large number of families/folds of a small size. Why are the distributions of protein families and folds skewed? Why are there only a limited number of protein families? Here, we employ an information theoretic approach to investigate the protein sequence-structure relationship that leads to...
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Connection matrix theory for discrete dynamical systems
PublicationIn [C] and [F1] the connection matrix theory for Morse decomposition is developedin the case of continuous dynamical systems. Our purpose is to study the case of discrete timedynamical systems.
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Effect of asymmetric fluid flow distribution on flow boiling in a microchannel heat sink – An experimental investigation
PublicationFlow boiling in microchannels is emerging as an exclusive cooling solution for miniaturized high-power electronic devices alongside having other high heat flux applications. Size miniaturization at microscale strangely increases heat transfer performance as well as flow boiling instabilities. Many flow boiling instabilities are interrelated and result from imperfect hydrodynamic conditions. One of such problems is flow maldistribution among...
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Platelets in fetal growth restriction: role of reactive oxygen species, oxygen metabolism, and aggregation.
PublicationFetal growth restriction (FGR) is mainly caused by failure of the uteroplacental unit. Theexact pathogenesis remains unclear. The cause is thought to be related to abnormal platelet activation,which may...
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Towards the boundary between easy and hard control problems in multicast Clos networks
PublicationIn this article we study 3-stage Clos networks with multicast calls in general and 2-cast calls, in particular. We investigate various sizes of input and output switches and discuss some routing problems involved in blocking states. To express our results in a formal way we introduce a model of hypergraph edge-coloring. A new class of bipartite hypergraphs corresponding to Clos networks is studied. We identify some polynomially...
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Index filtrations and Morse decomposition for discrete dynamical systems
PublicationOn a Morse decomposition of an isolated invariant set of a homeomorphism(discrete dynamical system) there are partial orderings defined by the homeomorphism.These are called admissible orderings of the...
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Technical monitoring system for a new part of Gdańsk Deepwater Container Terminal
PublicationDevelopment strategy for the port of Gdańsk assumes that , up to 2027, it will become the main transport centre in the Baltic Sea region. To this end , for many years work has been carried out to facilitate access to the port both by sea and land. One of the elements of extension of the port is the building of another terminal of Deepwater Container Terminal , Gdańsk. In this task, geotechnical operations aimed at appropriate...
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Social media as an opportunity or a blind alley in social communication and forest education? – Experiences from Poland
PublicationThe article characterizes the experiences, problems, and prospects for the development of forest education on social media, which in Poland has been carried out by the Państwowe Gospodarstwo Leśne Lasy Państwowe (National Forest Holding ‘State...
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Characterization of a single-stranded DNA-binding-like Protein from Nanoarchaeum equitans - a nucleic acid binding protein with broad substrate specificity
PublicationBackground SSB (single-stranded DNA-binding) proteins play an essential role in all living cells and viruses, as they are involved in processes connected with ssDNA metabolism. There has recently been an increasing interest in SSBs, since they can be applied in molecular biology techniques and analytical methods. Nanoarchaeum equitans, the only known representative of Archaea phylum Nanoarchaeota, is a hyperthermophilic, nanosized,...
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Pseudo-superparamagnetic behaviour of bariumhexaferrite particles
PublicationThe effect of hexadecyltrimethylammonium bromide (CTAB) addition on the crystal structure, morphology,and magnetic properties of co-precipitated hexagonal barium ferrite was investigated. For afixed amount ofsurfactant, different Fe3+concentrations and Fe3+/Ba2+ratios were used to optimize the formation ofsingle-phase barium ferrite particles. The results indicated that the obtained ferrite particles exhibitedcoercivity changes...
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Toward Safe and Efficient Recovery of Gas Munitions Dumped at Sea
PublicationThe proposal of a system for the safe recovery of unexploded ordnance (UXO), chemical weapons (CW) and chemicalwarfare agents (CWA) dumped at sea mainly after WWI and WWII is described in this work. The proposed solutionaddresses the tasks required to neutralise thousands of tons of dumped material and the currently available solutionsand proposed ideas. Requirements concerning the features of a recovery system are defined and...
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New Gene Markers Expressed in Porcine Oviductal Epithelial Cells Cultured Primary In Vitro Are Involved in Ontological Groups Representing Physiological Processes of Porcine Oocytes
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