Search results for: deinococcus - thermus

• RecA Proteins from Deinococcus geothermalis and Deinococcus murrayi - Cloning, Purification and Biochemical Characterisation

  Publication

  • M. Wanarska
  • B. Krawczyk
  • P. Hildebrandt
  • J. Kur

  - BMC MOLECULAR BIOLOGY - Year 2011

  Escherichia coli RecA plays a crucial role in recombinational processes, the induction of SOS response and mutagenic lesion bypasses. It has also been demonstrated that RecA protein is indispensable when it comes to the reassembly of shattered chromosomes in gamma-irradiated Deinococcus radiodurans, one of the most radiation-resistant organisms known. Moreover, some functional differences between E. coli and D. radiodurans RecA...

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• JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY

  Journals

  ISSN: 1388-6150 , eISSN: 1588-2926

• Extracellular proteolytic activity of Deinococcus geothermalis

  Publication

  • O. Pietrow-Tobiszewska
  • A. Panek
  • J. Synowiecki

  - AFRICAN JOURNAL OF BIOTECHNOLOGY - Year 2013

  Production of extracellular protease by extremophilic bacteria Deinococcus geothermalis cultivated in liquid media containing 0.1% (w/v) of peptone K, 0.1% yeast extract and 0.2% marine salt reached a maximum in 14 h of the cell growth at 45°C and pH 8.0. The enzyme was purified by a two-step procedure using fractionation by a graded ammonium sulphate precipitation technique and gel filtration on Sephadex G-100 column. Protease...

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• In vitro affinity of Deinococcus radiodurans MutS towards mismatched DNA exceeds that of its orthologues from Escherichia coli and Thermus thermophilus

  Publication

  • M. Banasik
  • A. Stanisławska-Sachadyn
  • E. Hildebrandt
  • P. Sachadyn

  - JOURNAL OF BIOTECHNOLOGY - Year 2017

  The mismatch binding protein MutS is responsible for the recognition of mispaired and unpaired bases, which is the initial step in DNA repair. Among the MutS proteins most extensively studied in vitro are those derived from Thermus thermophilus, Thermus aquaticus and Escherichia coli. Here, we present the first report on the in vitro examination of DNA mismatch binding activity of MutS protein from Deinococcus radiodurans and confront...

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• APPLIED THERMAL ENGINEERING

  Journals

  ISSN: 1359-4311

• Extremophile Deinococcus geothermalis as potential source of proteolytic enzymes

  Publication

  • O. Pietrow-Tobiszewska
  • A. Panek
  • J. Synowiecki

  - Acta Biochimica Polonica - Year 2011

  Among many extremozymes, thermophilic enzymes have attracted most attention during the past four decades. Such enzymes are of considerable industrial and biotechnological interest due to the fact that the enzymes are better suited for harsh industrial processes. There are many advantages of conducting industrial processes at high temperature, such as the increased solubility of many polymeric substrates, resulting in decreased...

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• Characterization of novel trehalose synthase from Deinococcus geothermalis.

  Publication

  • A. Panek
  • O. Pietrow-Tobiszewska
  • J. Synowiecki

  - Acta Biochimica Polonica - Year 2011

  The method of trehalose extraction from yeast or othernatural sources is unsuitable for industrial production becauseof its low yield and high cost. Thus, the trehalose synthesizingenzyme systems that originated from a few microorganisms have been investigated.

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• Properties and applications of thermostable proteases sourced from Deinococcus geothermalis.

  Publication

  • O. Pietrow-Tobiszewska
  • A. Panek

  - Year 2012

  The growing interest of extremophiles results from the fact that their enzymes arestable and active under harsh environment conditions. These type of biocatalysts are attractivedue to the fact that can be used in industrial processes that were previously regarded asincompatible with biological materials. Among extremozymes the largest group constitutethermozymes. Currently it is estimated that approximately 40% of enzymes used...

• Cloning, expression, purification and characterization of recombinant trehalose synthase from Deinococcus radiodurans.

  Publication

  • P. Filipkowski
  • K. Licznerska
  • A. Panek
  • O. Pietrow-Tobiszewska
  • J. Synowiecki

  - Acta Biochimica Polonica - Year 2010

  Trehalose (α-D-glucopyranosyl-1,1-α-D-glucopyranoside) is a nonreducing disacharide in which the two glucose molecules are linked trough a α-1,1-glycosidic bond. Trehalose is readily hydrolyzed to glucose and can be used as a reserve of that sugar in the cell. The presence of trehalose was found in the cells of fungi and yeasts, bacteria, nematodes, insects, eggs, pupae and some plants. The characteristics of trehalose make it...

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• Cloning, Expression and Purification of Wild-Type Trehalose Synthase from Deinococcus geothermalis

  Publication

  • A. Panek
  • P. Filipkowski
  • J. Synowiecki

  - Acta Biochimica Polonica - Year 2012

  The aim of this study was isolation and cloning of trehalose synthase gene derived from extremophilic microorganism to the expression vectors in the Tabor-Studier system and its expression in Rosetta(DE3)pLysS Escherichia coli cells. The second phase of the study consisted of proteins purification using an initial denaturation of host proteins and salting-out proteins by ammonium sulfate.

• Properties of recombinant trehalose synthase from Deinococcus radiodurans expressed in Escherichia coli

  Publication

  • P. Filipkowski
  • O. Pietrow-Tobiszewska
  • A. Panek
  • J. Synowiecki

  - Year 2012

  A trehalose synthase gene from Deinococcus radiodurans(DSMZ 20539)containing 1659 bp reading frame encoding 552 amino acids was amplified using PCR. The gene was finally ligated into pET30Ek/LIC vector and expressed after isopropyl β-d-thiogalactopyranoside induction in Escherichia coli (DE3) Rosetta pLysS. The recombinanttrehalose synthase (DraTreS) containing a His6-tag at the C-terminus was purified by metal affinity chromatography...

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• Identification and properties of the Deinococcus grandis andDeinococcus proteolyticus single-stranded DNA binding proteins (SSB)

  Publication

  • P. Filipkowski
  • J. Kur

  - Acta Biochimica Polonica - Year 2007

  W celu poznania właściwości biochemicznych białek SSB pochodzących z Deinococcus grandis (DgrSSB) i Deinococcus proteolyticus (DprSSB) sklonowano zaamplifikowane w reakcji PCR geny ssb w systemie ekspresyjnym Escherichia coli. Geny składają się z 891(DgrSSB) i 876 (DprSSB) nukleotydów kodujących odpowiednio 296 i 291 reszt aminokwasowych o teoretycznie wyznaczonej masie cząsteczkowej dla białek, równej 32,29 i 31,33 kDa. Sekwencja...

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• Novel thermostable single-stranded DNA-binding protein (SSB) from Deinococcus geothermalis

  Publication

  • P. Filipkowski
  • A. Duraj-Thatte
  • J. Kur

  - ARCHIVES OF MICROBIOLOGY - Year 2006

  W celu zbadania właściwości biochemicznych białka wiążącego się do jednoniciowego DNA (SSB) pochodzącego z Deinococcus geothermalis (DgeSSB), sklonowano gen ssb otrzymany w reakcji PCR, a następnie uzyskano wydajną biosyntezę białka DgeSSB. Gen składa się z 900 par zasad, kodujących białko złożone z 300 reszt aminokwasowych, o wyliczonej masie molekularnej równej 32.45 kDa. Sekwencja aminokwasowa wykazuje 43, 44% i 75% identyczności...

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• Expression of Deinococcus geothermalis Trehalose Synthase Gene in Escherichia coli and Its Enzymatic Properties

  Publication

  • P. Filipkowski
  • A. Panek
  • A. Felczykowska
  • O. Pietrow-Tobiszewska
  • J. Synowiecki

  - AFRICAN JOURNAL OF BIOTECHNOLOGY - Year 2012

  A novel trehalose synthase gene from Deinococcus geothermalis (DSMZ 11300) containing 1,692 bp reading-frame encoding 564 amino acids was amplified using PCR. The gene was ligated into pET30Ek/LIC vector and expressed after isopropyl alfa-D-thiogalactopyranoside induction in Escherichia coli BL21(DE3)pLysS. The recombinant trehalose synthase (DgeoTreS) containing a His6 tag at the C-terminus was purified by metal affinity chromatography...

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• A highly thermostable, homodimeric single-stranded DNA-binding protein from Deinococcus radiopugnans

  Publication

  • P. Filipkowski
  • M. Koziatek
  • J. Kur

  - EXTREMOPHILES - Year 2006

  We report the identification and characterization of the single-stranded DNA-binding protein (SSB) from the mesophile and highly radiation-resistant Deinococcus radiopugnans (DrpSSB). PCR-derived DNA fragment containing the complete structural gene for DrpSSB protein was cloned and expressed in Escherichia coli. The gene consisting of an open reading frame of 900 nucleotides encodes a protein of 300 amino acids with a calculated...

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• Effects of the polyhistidine tag on kinetics and other properties of trehalose synthase from Deionococcus geothermalis

  Publication

  • A. Panek
  • O. Pietrow-Tobiszewska
  • P. Filipkowski
  • J. Synowiecki

  - Acta Biochimica Polonica - Year 2013

  Two recombinant trehalose synthases from Deinococcus geothermalis (DSMZ 11300) were compared. A significant influence of the artificial polyhistidine tag was observed in protein constitution. The recombinant trehalose synthase from D. geothermalis with His6 -tag has a higher K m value of 254 mM, in comparison with the wild-type trehalose synthase (K m 170 mM), and displayed a lower activity of maltose conversion when compared...

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• Identification, cloning, expression, and characterizationof a highly thermostable single-stranded-DNA-binding protein (SSB) from Deinococcus murrayi

  Publication

  • P. Filipkowski
  • A. Duraj-Thatte
  • J. Kur

  - PROTEIN EXPRESSION AND PURIFICATION - Year 2007

  Zidentyfikowano i scharakteryzowano białko SSB-podobne, pochodzące z Deinococcus murrayi (DmuSSB). Otrzymany w reakcji PCR fragment zawierający kompletny gen ssb sklonowano w systemie ekspresyjnym Escherichia coli. Gen składa się z 826 nt, kodujących 276 reszt aminokwasowych z wyliczoną teoretycznie masą cząsteczkową monomeru równą 30,14 kDa. DmuSSB zawiera dwie domeny wiążące jednoniciowe DNA OBna monomer I funkcjonuje jako homodimer....

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• Case Studies in Thermal Engineering

  Journals

  ISSN: 2214-157X

• INTERNATIONAL JOURNAL OF THERMAL SCIENCES

  Journals

  ISSN: 1290-0729 , eISSN: 1778-4166

• Hermes

  Journals

  ISSN: 0018-0777 , eISSN: 2365-3116

• Production of trehalose by intramolecular transglucosylation of maltose catalysed by a new enzyme from Thermus thermophilus hb-8

  Publication

  • A. Zdziebło
  • J. Synowiecki

  - FOOD CHEMISTRY - Year 2006

  Thermus thermophilus HB-8 jest źródłem syntazy trehalozy katalizującej konwersję maltozy. Aktywność specyficzna wytwarzania trehalozy w reakcji katalizowanej ekstraktem białek komórkowych wymienionej bakterii wynosi 0,1 U/mg białka i frakcjonowanie białek przez wysalanie siarczanem amonu powoduje około 3,5-krotne zwiększenie aktywności specyficznej. Optymalna temperatura konwersji maltozy wynosi 65 st.C i w 85 st.C zostaje zachowane...

• EXPERIMENTAL THERMAL AND FLUID SCIENCE

  Journals

  ISSN: 0894-1777 , eISSN: 1879-2286

• Thermal Science

  Journals

  ISSN: 0354-9836 , eISSN: 2334-7163

• Potential Use of Silica Nanoparticles for the Microbial Stabilisation of Wine: An In Vitro Study Using Oenococcus oeni as a Model

  Publication

  • K. Pachnowska
  • K. Cendrowski
  • X. Stachurska
  • P. Nawrotek
  • A. Augustyniak
  • E. Mijowska

  - Foods - Year 2020

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• HERMES (France)

  Journals

  ISSN: 0767-9513

• Sławomir Milewski prof. dr hab. inż.

  People

  Department of Pharmaceutical Technology and Biochemistry

  Sławomir Milewski, born in 1955 in Puck, Poland, graduated in 1979 in Chemistry from the Faculty of Chemistry, Gdańsk University of Technology (GUT). In 1984 was employed at the Department of Pharmaceutical Technology and Biochemistry. In 1985 he got his PhD, in 1994 became a DSc (habilitation) and in 2002 got the professorship in chemical sciences. Currently he is a full professor and Head of the Department of Pharmaceutical Technology...

• Computational Thermal Sciences

  Journals

  ISSN: 1940-2503 , eISSN: 1940-2554

• Journal of Thermal Engineering

  Journals

  ISSN: 2148-7847

• JOURNAL OF THERMAL BIOLOGY

  Journals

  ISSN: 0306-4565

• Identification and characterization of single-stranded-DNA-binding proteins from Thermus thermophilus and Thermus aquaticus - new arrangement of binding domains

  Publication

  • S. Dąbrowski
  • M. Olszewski
  • R. Piątek
  • A. Brillowska-Dąbrowska
  • G. Konopa
  • J. Kur

  - MICROBIOLOGY-SGM - Year 2002

  Określono molekularne właściwości białek SSB-podobnych z Thermus thermophi-lus i T. aquaticus. Zbadano aktywność białek oraz termostabilność. Ponadto, dowiedziono homodimeryczność obu białek i ustalono minimalną sekwencję wiążącą ssDNA.

• Construction and purification of his6-Thermus thermophilus MutS protein

  Publication

  • A. Stanisławska-Sachadyn
  • P. Sachadyn
  • R. Jędrzejczak
  • J. Kur

  - PROTEIN EXPRESSION AND PURIFICATION - Year 2003

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• Construction and purification of his6-Thermus thermophilus MutS protein.

  Publication

  • A. Stanisławska-Sachadyn
  • P. Sachadyn
  • R. Jędrzejczak
  • J. Kur

  - PROTEIN EXPRESSION AND PURIFICATION - Year 2003

  Gen mutS z termofilnej bakterii Thermus thermophilus zamplifikowano przy użyciu PCR, sklonowano i przeprowadzono jego ekspresję w E. coli. Rekombinantowe białko MutS zawierające oligohistydynową domenę na N-końcu oczyszczono w jednoetapowej procedurze przy wykorzystaniu chromatografii powinowactwaNi(2+). Zdolność do rozpoznania niekomplementarności białka his(6)-MutS potwierdzono w eksperymentach ochrony DNA przed trawieniem...

• Thermal utilization of meat and bone meals. Performance analysis in terms of drying process, pyrolysis and kinetics of volatiles combustion

  Publication

  • M. Kantorek
  • K. Jesionek
  • S. Polesek-Karczewska
  • P. Ziółkowski
  • J. Badur

  - FUEL - Year 2019

  A development of technology of thermal utilization requires a thorough knowledge of the physicochemical properties, as well as kinetics of combustion of volatiles and char. Due to waste management regulations the meat and bone meal (MBM) is regarded as biofuel constituting one of the alternatives to fossil fuels in the energy market. MBM is a heterogeneous material that may consist of several different fractions. The extensive...

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• Emerging Themes in Epidemiology

  Journals

  ISSN: 1742-7622

• THEMIS POLSKA NOVA

  Journals

  ISSN: 2084-4522

• Revista Cientifica Hermes

  Journals

  ISSN: 2175-0556

• Journal of Thermal Science

  Journals

  ISSN: 1003-2169 , eISSN: 1993-033X

• Characterization of volatile compounds, structural, thermal and physico-mechanical properties of cross-linked polyethylene foams degraded thermo-mechanically at variable times

  Publication

  • K. Formela
  • M. Wołosiak
  • M. Klein
  • S. Wang

  - POLYMER DEGRADATION AND STABILITY - Year 2016

  Waste cross-linked polyethylene foam (wXLPE) was thermo-mechanically degraded at variable time using internal batch mixer. The progress of wXLPE degradation has been investigated by using a simultaneous thermogravimetric/differential scanning calorimetry analyzer coupled with Fourier transform infrared spectroscopy, swelling measurements, tensile tests and scanning electron microscopy. Volatile organic compounds generated during...

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• Thermal Science and Engineering Progress

  Journals

  eISSN: 2451-9049

• JOURNAL OF THERMAL SPRAY TECHNOLOGY

  Journals

  ISSN: 1059-9630 , eISSN: 1544-1016

• Journal of Thermal Science and Technology

  Journals

  ISSN: 1880-5566

• Characterization of DNA Polymerase from Thermus thermophilus MAT72 Phage Tt72

  Publication

  • S. Dorawa
  • M. Plotka
  • A. Kaczorowska
  • O. Fridjonsson
  • G. Hreggvidsson
  • A. Aevarsson
  • T. Kaczorowski

  - Year 2020

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• Charakterystyka bakterii rodzaju Thermus i ich przydatność w biotechnologii

  Publication

  • I. Sinkiewicz
  • J. Synowiecki

  - BioTechnologia - Year 2009

  Gram ujemne, termofilne bakterie rodzaju Thermus o optymalnych temperaturach wzrostu mieszczących się w zakresie 55-85 C są przydatne jako źródło nukleaz, polimeraz DNA oraz wielu innych termostabilnych enzymów. W przedstawionym artykule wymieniono występowanie i morfologię bakterii rodzaju Thermus, sposoby odżywiania i warunki ich wzrostu, budowę struktur powierzchniowych, sposoby adaptacji do wzrostu w podwyższonej temperaturze...

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• Ocena przydatności bakterii Thermus ruber jako źródła syntazy trehalozy

  Publication

  • I. Sinkiewicz
  • J. Synowiecki

  - BioTechnologia - Year 2008

  Wykazano, że termofilna bakteria Thermus ruber jest źródłem syntazy trehalozy,katalizującej wewnątrzcząsteczkową transglikozylację maltozy. Wymieniony enzym przejawia największą aktywność w temp. 65 st. C przy pH 6,5. Najlepszą wydajność syntazy trehalozy uzyskiwano podczas hodowli bakterii w 55 st. C na podłożu zawierającym 0,5% peptonu 0,1% wyciągu drożdżowego i 0,5% maltozy lub skrobi.

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• Influence of external walls' thermal capacitance on indoor thermal comfort

  Publication

  • K. Arendt

  - Year 2013

  The doctoral thesis presents the results of numerical investigations on the influence of walls’ thermal capacitance on indoor thermal comfort. A proper set of thermal parameters of a building structure can considerably limit the heating / cooling demand, and passively increase the indoor thermal comfort. However the effect of building thermal capacitance on indoor conditions is complex and not always beneficial. Among a great number...

• 15th Conference on Calorimetry and Thermal Analysis

  Events

  08-09-2024 16:30 - 12-09-2024 13:30

  Registration for the workshops with Sponsors, which will take place during the free time on Tuesday, September 10, 2024, from 2:30 PM, will be conducted by signing up on a list at the registration desk. We kindly inform you that posters should be prepared in size B1 (707 x 1000 mm). Templates:pptx, pdf Please submit your presentations...

• Mariusz Kaczmarek dr hab. inż.

  People

  Gdańsk University of Technology, Department of Biomedical Engineering

  Received M.Sc., Eng. in Electronics in 1995 from Gdansk University of Technology, Ph.D. in Medical Electronics in 2003 and habilitation in Biocybernetics and Biomedical Engineering in 2017. He was an investigator in about 13 projects receiving a number of awards, including four best papers, practical innovations (7 medals and awards) and also the Andronicos G. Kantsios Award and Siemens Award. Main research activities: the issues...

• INFLUENCE OF NANOPARTICLE CONCENTRATION ON THERMAL PROPERTIES OF THERMAL OIL-MWCNT NANOFLUID

  Publication

  • J. Cieśliński
  • K. Ronewicz

  - Year 2015

  This paper deals with measurements of dynamic viscosity, thermal conductivity, electrical conductivity and pH of thermal oil-MWCNT (TO-MWCNT) nanofluid. Nanoparticles were tested at the concentration of 0.001%, 0.005%, 0.01%, 0.05%, and 0,1% by weight. Ultrasonic vibration was used in order to stabilize the dispersion of the nanoparticles. Thermal oil (TO) was selected as a base liquid, because of possible application in ORC systems...

• Influence of nanoparticle concentration on thermal properties of thermal oil-MWCNT nanofluid

  Publication

  • J. Cieśliński
  • K. Krygier
  • S. Smoleń

  - Applied Mechanics and Materials - Year 2016

  Results of the measurements of dynamic viscosity, thermal conductivity, electrical conductivity and pH of thermal oil-MWCNT (TO-MWCNT) nanofluid have been presented. Nanoparticles were tested at the concentration of 0.001%, 0.005%, 0.01%, 0.05%, and 0,1% by weight. Thermal oil (TO) was selected as a base liquid, because of possible application in ORC systems as an intermediate heating agent. Multi-walled carbon nanotubes were used...

• Użyteczność niektórych enzymów bakterii Thermus ruber w przetwórstwie sacharydów żywności

  Publication

  • I. Sinkiewicz

  - Year 2008