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Catalog Publications
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A standardised static in vitro digestion method suitable for food – an international consensus
PublicationSimulated gastro-intestinal digestion is widely employed in many fields of food and nutritional sciences, as conducting human trials are often costly, resource intensive, and ethically disputable. As a consequence, in vitro alternatives that determine endpoints such as the bioaccessibility of nutrients and non-nutrients or the digestibility of macronutrients (e.g. lipids, proteins and carbohydrates) are used for screening and building...
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AGREE—Analytical GREEnness Metric Approach and Software
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The role of bile salts in digestion
PublicationBile salts (BS) are bio-surfactants present in the gastrointestinal tract (GIT) that play a crucial role in the digestion and absorption of nutrients. The importance of BS for controlled release and transport of lipid soluble nutrients and drugs has recently stimulated scientific interest in these physiological compounds. BS are so-called facial amphiphiles showing a molecular structure that is very distinct from classical surfactants....
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Specificity of Infant Digestive Conditions: Some Clues for Developing Relevant In Vitro Models
PublicationDigestion of nutrients is an essential function of the newborn infant gut to allow growth and development and understanding infant digestive function is essential to optimize nutrition and oral drug delivery. Ethical considerations prohibit invasive in vivo trials and as a consequence in vitro assays are often conducted. However, the choice of in vitro model parameters are not supported by an exhaustive analysis of the literature...
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Fabrication of polyurethane and polyurethane based composite fibres by the electrospinning technique for soft tissue engineering of cardiovascular system
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Natural carbon-based quantum dots and their applications in drug delivery: A review
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Preparation, characterization and antimicrobial activity of polyvinyl alcohol/gum arabic/chitosan composite films incorporated with black pepper essential oil and ginger essential oil
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Theoretical description of the measuring method of instantaneous impedance spectra
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The Properties, Functions, and Use of Selenium Compounds in Living Organisms
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Use of Brassica Plants in the Phytoremediation and Biofumigation Processes
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Evaluation of corrosion inhibition of brass-118 in artificial seawater by benzotriazole using Dynamic EIS
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Crystal structures of the DsbG disulfide isomerase reveal an unstable disulfide
PublicationDsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. Here, we present two crystal structures of DsbG at 1.7- and 2.0-Angstrom resolution that are meant to represent the reduced and oxidized forms, respectively. The oxidized structure, however, reveals a mixture...
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Instantaneous impedance spectra of a non-stationary model electrical system
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Phosphorylation‐mediated regulation of heat shock response in Escherichia coli
PublicationEscherichia coli has two heat shock regulons under the transcriptional control of Esigma(32) and Esigma(E) RNA polymerases. These polymerases control the expression of genes, the products of which are needed for correct folding of proteins in the cytoplasm and the extracytoplasm respectively. In this study, we report that mutations in a tyrosine phosphatase-encoding gene led to decreased activity of these heat shock regulons. The...
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Selection of measurement frequency in Mott–Schottky analysis of passive layer on nickel
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Escherichia coli K-12 suppressor-free mutants lacking early glycosyltransferases and late acyltransferases. Minimal lipopolysaccharide structure and induction of envelope stress response.
PublicationTo elucidate the minimal lipopolysaccharide (LPS) structure needed for the viability of Escherichia coli, suppressor-free strains lacking either the 3-deoxy-d-manno-oct-2-ulosonic acid transferase waaA gene or derivatives of the heptosyltransferase I waaC deletion with lack of one or all late acyltransferases (lpxL/M/P) and/or various outer membrane biogenesis factors were constructed. Δ(waaC lpxL lpxM lpxP) and waaA mutants exhibited...
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Methods of Selenium Supplementation: Bioavailability and Determination of Selenium Compounds
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Transport of Particles in Intestinal Mucus under Simulated Infant and Adult Physiological Conditions: Impact of Mucus Structure and Extracellular DNA
PublicationThe final boundary between digested food and the cells that take up nutrients in the small intestine is a protective layer of mucus. In this work, the microstructural organization and permeability of the intestinal mucus have been determined under conditions simulating those of infant and adult human small intestines. As a model, we used the mucus from the proximal (jejunal) small intestines of piglets and adult pigs. Confocal...
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Mechanisms of Damage to DNA Labeled with Electrophilic Nucleobases Induced by Ionizing or UV Radiation
PublicationHypoxia—a hallmark of solid tumors—makes hypoxic cells radioresistant. On the other hand, DNA, the main target of anticancer therapy, is not sensitive to the near UV photons and hydrated electrons, one of the major products of water radiolysis under hypoxic conditions. A possible way to overcome these obstacles to the efficient radio- and photodynamic therapy of cancer is to sensitize the cellular DNA to electrons and/or ultraviolet...
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Investigation of pitting corrosion of stainless steel by means of acoustic emission and potentiodynamic methods
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Evaluation of pitting corrosion by means of dynamic electrochemical impedance spectroscopy
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Apple pectin complexes with whey protein isolate
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Dehydration converts DsbG crystal diffraction from low to high resolution
PublicationDiffraction quality crystals are essential for crystallographic studies of protein structure, and the production of poorly diffracting crystals is often regarded as a dead end in the process. Here we show a dramatic improvement of poorly diffracting DsbG crystals allowing high-resolution diffraction data measurement. Before dehydration, the crystals are fragile and the diffraction pattern is streaky, extending to 10 Å resolution....
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Effect of native air-formed oxidation on the corrosion behavior of AA 7075 aluminum alloys
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Synthesis of Thermally Stable Energetic 1,2,3-Triazole Derivatives
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The amplitude analysis of impedance spectra
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Impedance characterization of the process of polyaniline first redox transformation after aniline electropolymerization
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The Role of and the Place of Method Validation in the Quality Assurance and Quality Control (QA/QC) System
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Evaluation of cavitation erosion–corrosion degradation of mild steel by means of dynamic impedance spectroscopy in galvanostatic mode
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Complexing and Chelating Agents Immobilized on Silica Gel and Related Materials and Their Application for Sorption of Inorganic Species
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Determination of aminoglycoside antibiotics: Current status and future trends
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Carboxymethyl cellulose–gelatin complexes
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Thermomechanical reclaiming of ground tire rubber via extrusion at low temperature: Efficiency and limits
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The construction and use of a PCR internal control
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Corrosion rate measurements by non-linear electrochemical impedance spectroscopy
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Preparation and characterization of rigid polyurethane–polyglycerol nanocomposite foams
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Electrosynthesis of potato starch–whey protein isolate complexes
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Torsional elasticity and energetics of F1-ATPase
PublicationFoF1-ATPase is a rotary motor protein synthesizing ATP from ADP driven by a cross-membrane proton gradient. The proton flow through the membrane-embedded Fo generates the rotary torque that drives the rotation of the asymmetric shaft of F1. Mechanical energy of the rotating shaft is used by the F1 catalytic subunit to synthesize ATP. It was suggested that elastic power transmission with transient storage of energy in some compliant...
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Accelerated ageing of organic coating systems by thermal treatment
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The Reduced Folate Carrier (SLC19A1) c.80G>A Polymorphism is Associated with Red Cell Folate Concentrations Among Women
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Investigations of the passive layer cracking by means of Dynamic Electrochemical Impedance Spectroscopy
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DEIS assessment of AISI 304 stainless steel dissolution process in conditions of intergranular corrosion
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Poly(ε-Caprolactone)/Poly(Lactic Acid) Blends Compatibilized by Peroxide Initiators: Comparison of Two Strategies
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Keep It Flexible: Driving Macromolecular Rotary Motions in Atomistic Simulations with GROMACS
PublicationWe describe a versatile method to enforce the rotation of subsets of atoms, e.g., a protein subunit, in molecular dynamics (MD) simulations. In particular, we introduce a “flexible axis” technique that allows realistic flexible adaptions of both the rotary subunit as well as the local rotation axis during the simulation. A variety of useful rotation potentials were implemented for the GROMACS 4.5 MD package. Application to the...
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Electrochemical investigations of conductive coatings applied as anodes in cathodic protection of reinforced concrete
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Effect of environmental temperature variations on protective properties of organic coatings
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Pitting corrosion in steel and electrochemical noise intensity
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Microextraction Techniques Used in the Procedures for Determining Organomercury and Organotin Compounds in Environmental Samples
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Enzymatic cross-linking of β-lactoglobulin in solution and at air–water interface: Structural constraints
PublicationEffective and controlled use of cross-linking enzymes in structure engineering of food systems depends on characterization of the favorable conditions for enzyme-substrate complex and the limiting factors for the desired modification. In this respect, we analyzed the susceptibility of bovine β-lactoglobulin (BLG) to enzymatic cross-linking by Trichoderma reesei tyrosinase (TrTyr) and transglutaminase (TG). Changes in BLG molecular...
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Formation of carboxymethyl cellulose–casein complexes by electrosynthesis
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Linearization in impedance measurements
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Dynamic Electrochemical Impedance Spectroscopy (DEIS) as a Tool for Analyzing Surface Oxidation Processes on Boron-Doped Diamond Electrodes
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Generation of standard gaseous mixtures by thermal decomposition of surface compounds
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Amperometric sensing of chemical oxygen demand at glassy carbon and silicon electrodes modified with boron-doped diamond
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The transcobalamin (TCN2) 776C>G polymorphism affects homocysteine concentrations among subjects with low vitamin B12 status
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Molecular dynamics insights into protein‐glycosaminoglycan systems from microsecond‐scale simulations
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Use of Ginger Nanofibers for the Preparation of Cellulose Nanocomposites and Their Antimicrobial Activities
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DEIS evaluation of the relative effective surface area of AISI 304 stainless steel dissolution process in conditions of intergranular corrosion
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An evaluation of sucrose as a possible contaminant in e-liquids for electronic cigarettes by hydrophilic interaction liquid chromatography–tandem mass spectrometry
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Investigating the Impact of Curing System on Structure-Property Relationship of Natural Rubber Modified with Brewery By-Product and Ground Tire Rubber
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Silica xerogel–hydrogen peroxide composites: Their morphology, stability, and antimicrobial activity
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Ellipsometric study of oxide formation on Cu electrode in 0.1 M NaOH
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The time dependence of pit creation impedance spectra
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Liquefaction of alder wood as the source of renewable and sustainable polyols for preparation of polyurethane resins
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5-Thiocyanato-2′-deoxyuridine as a possible radiosensitizer: electron-induced formation of uracil-C5-thiyl radical and its dimerization
PublicationIn this work, we have synthesized 5-thiocyanato-2′-deoxyuridine (SCNdU) along with the C6-deuterated nucleobase 5-thiocyanatouracil (6-D-SCNU) and studied their reactions with radiation-produced electrons. ESR spectra in γ-irradiated nitrogen-saturated frozen homogeneous solutions (7.5 M LiCl in H2O or D2O) of these compounds show that electron-induced S–CN bond cleavage occurs to form a thiyl radical (dU-5-S˙ or 6-D-U-5-S˙) and...
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Fatty acyl benzamido antibacterials based on inhibition of DnaK-catalyzed protein folding
PublicationWe have reported that the hsp70 chaperone DnaK from Escherichia coli might assist protein folding by catalyzing the cis/trans isomerization of secondary amide peptide bonds in unfolded or partially folded proteins. In this study a series of fatty acylated benzamido inhibitors of the cis/trans isomerase activity of DnaK was developed and tested for antibacterial effects in E. coli MC4100 cells. Nα-[Tetradecanoyl-(4-aminomethylbenzoyl)]-l-asparagine...
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Quantification of key red blood cell folates from subjects with defined MTHFR 677C>T genotypes using stable isotope dilution liquid chromatography/mass spectrometry
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The influence of feed rate and shear forces on the devulcanization process of ground tire rubber (GTR) conducted in a co-rotating twin screw extruder
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Quality problems in determination of organic compounds in environmental samples, such as PAHs and PCBs
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Electrosynthesis of potato starch-casein complexes
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Dissection of σE‐dependent cell lysis in Escherichia coli: roles of RpoE regulators RseA, RseB and periplasmic folding catalyst PpiD
PublicationTo understand the mechanism of sigma(E)-dependent cell lysis, we examined the consequences of deletion derivatives of rpoE regulators rseA, rseB and rseC on sigma(E) transcription, on levels of free versus membrane-bound sigma(E) and on OMP-biogenesis limiting factor(s) that could impact cell lysis. RT-PCR showed that individual nonpolar DeltarseA and DeltarseB increased the rpoE expression to varying extents, with pronounced induction...
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Electrochemical characterization of Prussian blue type nickel hexacyanoferrate redox mediator for potential application as charge relay in dye-sensitized solar cells
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“Dilute & Shoot” approach for rapid determination of trace amounts of nicotine in zero-level e-liquids by reversed phase liquid chromatography and hydrophilic interactions liquid chromatography coupled with tandem mass spectrometry-electrospray ionization
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Study on the Structure-Property Dependences of Rigid PUR-PIR Foams Obtained from Marine Biomass-Based Biopolyol
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Organomercury Compounds in Environmental Samples: Emission Sources, Toxicity, Environmental Fate, and Determination
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Study of anticorrosion and microwave absorption properties of NiZn ferrite pigments
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Atomic force microscopy based approach to local impedance measurements of grain interiors and grain boundaries of sensitized AISI 304 stainless steel
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Investigations of pitting corrosion of magnesium by means of DEIS and acoustic emission
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Nuclear quantum effects in a HIV/cancer inhibitor: The case of ellipticine
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Molecular dynamics-based model of VEGF-A and its heparin interactions
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Dynamic electrochemical impedance spectroscopy measurements of passive layer cracking under static tensile stresses
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Surface Site Modulations by Conjugated Organic Molecules To Enhance Visible Light Activity of ZnO Nanostructures in Photocatalytic Water Splitting
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Real-Time Volatilomics: A Novel Approach for Analyzing Biological Samples
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Determination of tributyltin in marine sediment: Comit� Consultatif pour la Quantit� de Mati�re (CCQM) pilot study P-18 international intercomparison
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Local impedance imaging of boron-doped polycrystalline diamond thin films
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Instantaneous electrochemical impedance spectroscopy of electrode reactions
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Anti‐corrosion protection of chimneys and flue gas ducts
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Purification to Homogeneity of Candida albicans Glucosamine-6-phosphate Synthase Overexpressed in Escherichia coli
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Study of a method for the preparation of standard gas mixtures based on thermal decomposition of surface compounds. Application to isothiocyanates
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Harmonic Analysis Of Electrochemical and Corrosion Systems - A Review
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On-line assessment of oil quality during deep frying using an electronic nose and proton transfer reaction mass spectrometry
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Frequency dispersion of harmonic components of the current of an electrode process
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An insertion/deletion polymorphism of the dihydrofolate reductase (DHFR) gene is associated with serum and red blood cell folate concentrations in women
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Problems of PAH quantification by GC–MS method using isotope-labelled standards
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Influence of the analyzing window on electrode impedance measurement by the continuous frequency scanning method
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Possibility of thermal imaging use in studies of natural convection heat transfer on the example of an isothermal vertical plate
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Application of dynamic impedance spectroscopy to atomic force microscopy
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Use of porous glass and silica gel as support media of a surface compound for generation of analytes in gaseous standard mixtures. New method for the determination of the amount of analyte generated
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Angiotensin converting enzyme gene polymorphism is associated with severity of coronary artery disease in men with high total cholesterol levels
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Construction, purification, and functional characterization of His-tagged Candida albicans glucosamine-6-phosphate synthase expressed in Escherichia coli
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