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Wyniki wyszukiwania dla: SAMPLE PREPARATION METHOD HUMAN BREAST MILK LIPID EXTRACTION METABOLITE EXTRACTION METABOLOMICS LIPIDOMICS HUMAN BREAST MILK CONTAMINANTS
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Electroporation with Cisplatin against Metastatic Pancreatic Cancer:In VitroStudy on Human Primary Cell Culture
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Combination of therapy with 5-fluorouracil and cisplatin with electroporation in human ovarian carcinoma model in vitro
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The modulatory effect of green tea catechin on drug resistance in human ovarian cancer cells
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Structure–bioavailability relationship study of genistein derivatives with antiproliferative activity on human cancer cell
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Synthesis of New Hydrazone Derivatives and Evaluation of their Efficacy as Proliferation Inhibitors in Human Cancer Cells
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Anticancer properties of ester derivatives of betulin in human metastatic melanoma cells (Me-45)
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Novel Potential Binding Sites for Selective Inhibitor Design of Human Soluble Epoxide Hydrolase
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Ornamental Flowers Grown in Human Surroundings as a Source of Anthocyanins with High Anti-Inflammatory Properties
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Lysosome Alterations in the Human Epithelial Cell Line HaCaT and Skin Specimens: Relevance to Psoriasis
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Non-steroidal anti-inflammatory drugs are safe with respect to the transcriptome of human dermal fibroblasts
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MicroRNA expression profile analysis in human skeletal muscle tissue: Selection of critical reference
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PELP1 and SRC kinase as important molecules in the estrogen-mediated pathway in human testis and epididymis
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Postmortem analysis of human bone marrow aspirate - Quantitative determination of SSRI and SNRI drugs
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The role of colour preattentive processing in human–computer interaction task efficiency: A preliminary study
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Bio-enriched Pleurotus mushrooms for deficiency control and improved antioxidative protection of human platelets?
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The effects of p53 gene inactivation on mutant proteome expression in a human melanoma cell model
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Sensitivity of personalized ventilation air terminal device geometry with regard to a human thermal sensation
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The influence of different expression of CYP3A4 izoenzymes on metabolic pathway derivatives in human hepatoma cells
PublikacjaCelem przeprowadzonych badań było określenie różnic w metabolizmie pochodnych C-857 i C-1748 w komórkach ludzkiego nowotworu wątroby HepG2 oraz określenie wpływu na ich biotransformację w podwyższonej ekspresji izoenzymu CYP3A4 w tejże linii. Uzyskane wyniki wskazały, że związek C-857, jak również C-1748 metabolizuje w komórkach HepG2 oraz Hep3A4 do wielu reaktywnych, niestabilnych metabolitów. Różnice w biotransformacji były bardzo...
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Proteomic-Biostatistic Integrated Approach for Finding the Underlying Molecular Determinants of Hypertension in Human Plasma
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Serum of obstructive sleep apnea patients impairs human coronary endothelial cell migration
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Influence of adalimumab on interleukin 12/23 signalling pathways in human keratinocytes treated with lipopolysaccharide A
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Induction of apoptosis by triazoloacridinone derivative - C-1533 in HL60 and MOLT4 human leukemia cells.
PublikacjaPrzeprowadzone badania miały na celu określenie zdolności triazoloakrydonu C-1533 do indukcji śmierci komórek nowotworowych MOLT4 i HL60 na drodze apoptozy. Badanie zjawiska apoptozy przeprowadzono z wykorzystaniem techniki cytofluorymetrii przepływowej, mikroskopii fluorescencyjnej i elektroforezy agarozowej. Badania wykazały, że zarówno w komórkach MOLT4 jak i HL60, spadek mitochondrialnego potencjału błonowego oraz translokacja...
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The effect of new non-cross resistant antitumor agents on the energy state of human erythrocytes
PublikacjaPrzebadano pochodne benzoperimidynowe i antrapirydonowe odnośnie ich wpływu na stan energetyczny ludzkich erytrocytów. Stwierdzono, że poziom nukleotydów jak i wartość AEC erytrocytów nie zmienił się w ciągu 24 h inkubacji z powyższymi związkami. Jest to wynik obiecujący w odniesieniu do ich zastosowania jako związków aktywnych w pokonywaniu oporności wielolekowej.
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A methodology for incorporating human factors in fuzzy-probabilistic modelling and risk analysis of industrial systems
PublikacjaPraca dotyczy wybranych zagadnień rozmyto-probabilistycznych modelowania złożonych systemów przemysłowych. Do obliczeń związanych z analizą ryzyka stosuje się diagramy wpływu i hierarchiczny proces decyzyjny.
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The protocol for using elastic wall model in modeling blood flow within human artery
PublikacjaMedical diagnostic tools will play a major role in the future for an effective patient treatment and reduction their mortality, related to the cardiovascular system diseases (CVDs). There is an urgent need for developing diagnostic procedure to be robust, reliable, accurate and efficient, in the framework of a paradigm shift. Application of numerical techniques is seen as a perspective tool for such purpose. Nevertheless, existing...
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Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells
PublikacjaAdipose-derived stem cells (ASCs) have become an important research model in regenerative medicine. However, there are controversies regarding the impact of prolonged cell culture on the ASCs phenotype and their differentiation potential. Hence, we studied 10 clinical ASCs replicates from plastic and oncological surgery patients, in six-passage FBS supplemented cultures. We quantified basic mesenchymal cell surface marker transcripts...
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Wavelet transform analysis to assess oscillations in pial artery pulsation at the human cardiac frequency
PublikacjaPial artery adjustments to changes in blood pressure (BP)may last only seconds in humans. Using a novelmethod called near-infrared transillumination backscattering sounding (NIR-T/BSS) that allows for the non-invasive measurement of pial artery pulsation (cc-TQ) in humans, we aimed to assess the relationship between spontaneous oscillations in BP and cc-TQ at frequencies between 0.5 Hz and 5 Hz. We hypothesized that analysis of...
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A Study in Experimental Methods of Human-Computer Communication for Patients After Severe Brain Injuries
PublikacjaExperimental research in the domain of multimedia technology applied to medical practice is discussed, employing a prototype of integrated multimodal system to assist diagnosis and polysensory stimulation of patients after severe brain injury. The system being developed includes among others: eye gaze tracker, and EEG monitoring of non-communicating patients after severe brain injuries. The proposed solutions are used for collecting...
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Human factor and functional Safety analysis = Czynniki ludzkie i analiza bezpieczeństwa funkcjonalnego
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MIC1-MAG1-SAG1 chimeric protein, a most effective antigen for detection of human toxoplasmosis
PublikacjaThis study describes a Toxoplasma gondii IgG ELISA test based on a new chimeric antigen containing three immunodominant regions from MIC1, MAG1 and SAG1 proteins of a parasite and shows that this test is useful for diagnostic purposes and may replace the lysed and whole-cell antigens.
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Protection and viability of fruit seeds oils by nanostructured lipid carrier (NLC) nanosuspensions
PublikacjaIn this paper, we focused on the development of nanostructured lipid carriers (NLCs) for dermal application. The NLC matrix was designed as a protective reservoir of biological active compounds that naturally occur in domestic fruit seed oils. Over the years, emulsions, as a popular physicochemical form of personal care products, were refined in order to obtain the best possible penetration into the skin of any bioactive compound...
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Optimizing of MALDI-ToF-based low-molecular-weight serum proteome pattern analysis in detection of breast cancer patients; the effect of albumin removal on classification performance.
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Influence of oxygenation of constructed wetlands for effectiveness of contaminants removal
PublikacjaBadania prowadzono w trzech hybrydowych systemach hydrofitowych (HSH): Wiklinie, Wieszynie i Wiedersbergu. Obiekty charakteryzowały się różnym ładunkiem doprowadzanej SO. Określenie jakości SO w ściekach polegało na przeprowadzeniu analizy specjacji substancji organicznej zgodnie z metodyką podaną w niemieckich wytycznych ATV-131. Na podstawie analizy frakcji ChZT wykazano, że szybkość rozkładu SO (k) w złożach była determinowana...
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Development of a solventless stir bar sorptive extraction/thermal desorption large volume injection capillary gas chromatographic-mass spectrometric method for ultra-trace determination of pyrethroids pesticides in river and tap water samples
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On the possibility of using liguid or lipid, lipid like-polimer membrane systems as taste sensor
PublikacjaZastosowano membrany ciekłe w oscylatorach zawierających oleinian sodu i substancje należące do czterech klas smaku. Wpływają one na charakterystyki oscylacyjne potencjału elektrycznego miedzy fazami wodnymi. Otrzymane atraktory są różne w kształcie i dwu wymiarowej powierzchni. Powierzchnia ta zależy także od stężenia sacharozy w układzie. Mogą one byc użyte jako ''odcisk palca'' do indentyfikacji odpowiednije substancji smakowej....
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Different detection and stacking techniques in capillary electrophoresis for metabolomics
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Perspectives and challenges in extracellular vesicles untargeted metabolomics analysis
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Variable Ratio Sample Rate Conversion Based on Fractional Delay Filter
PublikacjaIn this paper a sample rate conversion algorithm which allows for continuously changing resampling ratio has been presented. The proposed implementation is based on a variable fractional delay filter which is implemented by means of a Farrow structure. Coefficients of this structure are computed on the basis of fractional delay filters which are designed using the offset window method. The proposed approach allows us to freely...
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Preparation and characterization of rigid polyurethane-polyglycerolnanocomposite foams
PublikacjaThis work reports on the preparation of polyurethane-polyisocyanurate (PUR-PIR) foams containing different polyglycerols and layered silicate nanoclays. The rigid polyurethane foams were obtained in a laboratory scale, in a single step method, from a two-component system with a NCO to OH groups ratio equal to two. The reaction mixture consisted of the proper amounts of a commercial oligoetherpolyol, polyglycerol, catalysts, water,...
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Determination of Terpene Profile in Cape gooseberry (Physalis peruviana L.) with Use of HS-SPME/GCxGC-TOFMS Method
PublikacjaFresh fruits and products made of them, especially fruit juice, provide the basic energetic substances (simple and complex sugars) and are a source of health beneficial vitamins, macro and microelements, antioxidants and compounds which are classified together with essential oils (for example terpenes), which positively affect human s health. The terpenes profile of cape gooseberry was determined by headspace solid--phase microextraction...
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Preparation and characterization of dummy-template molecularly imprinted polymers as potential sorbents for the recognition of selected polybrominated diphenyl ethers
PublikacjaThe main aim of this work was to conduct the preliminary/basic research concerning the preparation process of a new dummy molecularly imprinted polymer (DMIP) materials. Developed DMIPs were proposed as a sorption material in solid-phase extraction (SPE) technique for recognition of selected low mass polybrominated diphenyl ethers (PBDEs) e PBDE-47 and PBDE-99. Four new DMIPs were synthesized employing bulky polymerization technique...
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Determination of parent and methylated polycyclic aromatic hydrocarbons in water samples by dispersive liquid–liquid microextraction–two dimensional gas chromatography–time-of-flight mass spectrometry
PublikacjaPolycyclic aromatic hydrocarbons (PAHs) are contaminants frequently found in environmental waters. Owing to their toxic effects, it is important to develop analytical methodologies that allow their fast and reliable determination. Dispersive liquid–liquid microextraction (DLLME) allows a quick extraction of analytes with little organic solvent consumption. The best results regarding sample preparation are obtained when 40 mL of...
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Method for the simultaneous determination of monoaromatic and polycyclic aromatic hydrocarbons in industrial effluents using dispersive liquid-liquid microextraction with GC-MS
PublikacjaWe present a new method for simultaneous determination of 22 monoaromatic and polycyclic aromatic hydrocarbons in postoxidative effluents from the production of petroleum bitumen using dispersive liquid-liquid microextraction coupled to gas chromatography and mass spectrometry. The eight extraction parameters including the type and volume of extraction and disperser solvent, pH, salting out effect, extraction and centrifugation...
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Lipid peroxidation in the onset of neuroborreliosis
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Lipid peroxidation in the pathogenesis of neuroborreliosis
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Cost-Effective and Sufficiently Precise Integration Method Adapted to the FEM Calculations of Bone Tissue
PublikacjaThe technique of Young’s modulus variation in the finite element is not spread in biomechanics. Our future goal is to adapt this technique to bone tissue strength calculations. The aim of this paper is to present the necessary studies of the element’s integration method that takes into account changes in material properties. For research purposes, a virtual sample with the size and distribution of mechanical properties similar...
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Discriminating macromolecular interactions based on an impedimetric fingerprint supported by multivariate data analysis for rapid and label-free Escherichia coli recognition in human urine
PublikacjaThis manuscript presents a novel approach to address the challenges of electrode fouling and highly complex electrode nanoarchitecture, which are primary concerns for biosensors operating in real environments. The proposed approach utilizes multiparametric impedance discriminant analysis (MIDA) to obtain a fingerprint of the macromolecular interactions on flat glassy carbon surfaces, achieved through self-organized, drop-cast,...
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Preparation and characterisation of solution-cast Nafion membrane
PublikacjaNafion is a commercial name for a perfluorosulfonic polymer, which has been developed in 1962 by Dupont de Nemours. This polymer consists of a polytetrafluoroethylene backbone and regular spaced long perfluorovinyl ether pendant side chains terminated by a sulfonate ionic group. These sulfonate groups are responsible for cation-exchange properties of Nafion. Thanks to the ion-exchange properties, water permeability, good chemical,...
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Completing Autophagy: Formation and Degradation of the Autophagic Body and Metabolite Salvage in Plants
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Nowoczesne techniki przygotowania próbki do oznaczania analitów organicznych z zastosowaniem chromatografii gazowej. Część I. Podstawy teoretyczne ekstrakcji. Modern techniques of preparation of samples for determining organic analytes by gas chromatography. Part I. Theoretical principles of extraction.
PublikacjaPrzedstawiono podstawy teoretyczne technik izolacji i wzbogacania analitów organicznych z próbek gazowych oraz ciekłych. Już pobieżna analiza literatury dotyczącej omawianych zagadnień, pokazuje jak różnorodne i bogate spektrum technik jest stosowane na etapie przygotowania próbki. Z drugiej strony, z punktu widzenia podstaw fizykochemicznych, większość technik opiera się na dwóch zjawiskach: adsorpcji analitów na medium zatrzymującym...