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Wyniki wyszukiwania dla: DNA REPAIR ENZYMES
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Sequences of large subunit ribosomal RNA gene, partial sequence encoding D1/D2 domain of psychrotolerant pigment-producing yeast-like fungi
Dane BadawczeDNA fragments encoding D1/D2 domain of large subunit ribosomal RNA of psychrotolerant pigment-producing yeast-like fungi named Red, Pink and Black were PCR amplified using NL1 5’ GCATATCAATAAGCGGAGGAAAAG 3’ and NL4 5’ GGTCCGTGTTTCAAGACGG 3’ primers and sequenced by Sanger method using the same primers.
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Renovation works in buildings in the area of former defensive fortifications
Publikacjahe paperpresents the Complex of Buildings which was created in Gdańsk as a result of the reconstruction and development of the remains of the defensive fortifications of Redita Napoleońska. Some of the buildings of the Building Complex, after many years of operation, were in an emergency condition and required urgent renovation and repair work. The papercontains a detailed analysis of the technicalcondition of individual...
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Properties and applications of thermostable proteases sourced from Deinococcus geothermalis.
PublikacjaThe growing interest of extremophiles results from the fact that their enzymes arestable and active under harsh environment conditions. These type of biocatalysts are attractivedue to the fact that can be used in industrial processes that were previously regarded asincompatible with biological materials. Among extremozymes the largest group constitutethermozymes. Currently it is estimated that approximately 40% of enzymes used...
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Polyp of stomach and duodenum - Female, 46 - Tissue image [7110729594548961]
Dane BadawczeThis is the histopathological image of STOMACH tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Polyp of stomach and duodenum - Female, 38 - Tissue image [7110729594548871]
Dane BadawczeThis is the histopathological image of STOMACH tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Sławomir Milewski prof. dr hab. inż.
OsobySławomir Milewski, urodzony w 1955 r. w Pucku (woj. pomorskie), ukończył w 1979 r. studia magisterskie na kierunku Chemia, w specjalności Chemia i Technologia Organiczna, na Wydziale Chemicznym PG. Po ukończeniu Studium Doktoranckiego w 1984 r., został zatrudniony na macierzystym wydziale w Katedrze Technologii Leków i Biochemii, początkowo na stanowisku naukowo-technicznym, a od r. 1986 jako nauczyciel akademicki. W 1985 r. uzyskał...
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Inhibitors of amino acids biosynthesis as antifungal agents
PublikacjaFungal microorganisms, including the human pathogenic yeast and filamentous fungi, are able to synthesize all proteinogenic amino acids, including nine that are essential for humans. A number of enzymes catalyzing particular steps of human-essential amino acid biosynthesis are fungi specific. Numerous studies have shown that auxotrophic mutants of human pathogenic fungi impaired in biosynthesis of particular amino acids exhibit...
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New generation of analytical tests based on the assessment of enzymatic and nuclear receptor activity changes induced by environmental pollutants
PublikacjaAnalytical methods show great potential in biological tests. The analysis of biological response that results from environmental pollutant exposure allows: (i) prediction of the risk of toxic effects and (ii) provision of the background for the development of markers of the toxicants presence. Bioanalytical tests based on changes in enzymatic activity and nuclear receptor action provide extremely high specificity and sensitivity....
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Characteristic and specificity of protease activity of thermophilic bacteria Meiothermus ruber
PublikacjaThe primary purpose of research was to establish the possibility of using thermophilic bacteria Meiothermus ruber as a source of proteolytic enzymes. The main reason of searching new proteases is their commercial value especially in production of detergents, food, pharmaceuticals, leather, diagnostics or waste management. The development of this type processes will be possible by utilization of enzymes which have unique characteristics...
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Enzymatic cross-linking of β-lactoglobulin in solution and at air–water interface: Structural constraints
PublikacjaEffective and controlled use of cross-linking enzymes in structure engineering of food systems depends on characterization of the favorable conditions for enzyme-substrate complex and the limiting factors for the desired modification. In this respect, we analyzed the susceptibility of bovine β-lactoglobulin (BLG) to enzymatic cross-linking by Trichoderma reesei tyrosinase (TrTyr) and transglutaminase (TG). Changes in BLG molecular...
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Meiothermus ruber cells as β-galactosidase activity biocatalyst
PublikacjaThe current study allowed to establish the possibility of using whole cells of thermophilic bacteria Meiothermus ruber as a biocatalyst with β-galactosidase activity. β-galactosidases are used for hydrolysis of lactose as well as for oligosaccharides synthesis. The advantages of using whole bacterial cells catalysis is not only elimination of tedious, expensive protein isolation and/or purification but also stabilization of enzymes...
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Wzmocnienie przestrzennych dźwigarów kratowych z zastosowaniem dodatkowego podparcia i sprężenia
PublikacjaPodczas obowiązkowego przeglądu technicznego obiektu budowlanego stwierdzone zostały deformacje stalowych prętów wykratowania przestrzennych dźwigarów dachowych. Opinie techniczne dotyczące stanu technicznego potwierdziły wstępnie wykryte nieprawidłowości i nakazywały podjęcie kroków zaradczych w postaci opracowania koncepcji naprawy, a w jej ślad projektu budowlanego i wykonawczego. Do czasu naprawy obiekt został dopuszczony do...
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Przyczyny uszkodzeń i sposób naprawy posadzki ze styrobetonu
PublikacjaW artykule opisano uszkodzenia, badania i sposób naprawy posadzki wykonanej na istniejącym stropie żelbetowym o powierzchni ok. 1050 m2 i podłożu ze styrobetonu. W posadzce stwierdzono znaczne uszkodzenia w postaci zarysowań, odspojeń i nierówności. W celu określenia ich przyczyn wykonano badania in situ oraz badania laboratoryjne. Stwierdzono, że przyczyną powstania uszkodzeń są błędy wykonawcze. Wykonano próbną naprawę uszkodzeń...
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Biological Activity of Tea-Chokeberry and Chokeberry Fermented Beverages
PublikacjaFermented beverages, such as kombucha are gaining more and more popularity due to their beneficial effects on human health. These products are obtained by the fermentation of tea infusion using so called tea fungus. This microbial consortium can also be used to prepare fermented fruit beverages. Therefore, the aim of this study was to determine the changes in the antioxidant profile of tea-chokeberry and chokeberry beverages...
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Molecular targets for antifungals in amino acid and protein biosynthetic pathways
PublikacjaFungi cause death of over 1.5 million people every year, while cutaneous mycoses are among the most common infections in the world. Mycoses vary greatly in severity, there are long-term skin (ringworm), nail or hair infections (tinea capitis), recurrent like vaginal candidiasis or severe, life-threatening systemic, multiorgan infections. In the last few years, increasing importance is attached to the health and economic problems...
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Mirosław Andrusiewicz prof. dr hab. n. med. i n. o zdr.
OsobyPosiadane dyplomy, stopnie naukowe lub artystyczne ̶ stopień doktora habilitowanego nauk medycznych (dyscyplina biologia medyczna) z dnia 4 grudnia 2017 r. Tytuł osiągnięcia naukowego: „Analiza wybranych genów związanych z przebiegiem zmian patologicznych w komórkach wywodzących się z żeńskich wewnętrznych narządów płciowych”; Uniwersytet Medyczny im. Karola Marcinkowskiego w Poznaniu, Wydział Lekarski II; recenzenci: Prof....
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Polyp of stomach and duodenum - Female, 24 - Tissue image [7110729594544271]
Dane BadawczeThis is the histopathological image of STOMACH tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Sensivity of echo envelope fractal dimension to bottom type and bottom depth.
PublikacjaPrzedstawiono wynik badania zależności pomiędzy wymiarem fraktalnym obwiedni echa akustycznego od dna a rodzajem dna na wpływ głębokości dna na tę zależność. Wykorzystano sygnały ech otrzymane w wyniku numerycznych symulacji rozpraszania na granicy woda dno dla różnych głębokości dna.
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Example of utilization of permeabilized microbial cells
PublikacjaThe benefits of using whole bacterial cells not only exclude expensive, laborious protein isolation and purification but also stabilize enzymes by cytosol components. Increase in activity of the cells can be achieved by cells permeabilization.
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Transcriptomic responses to wounding: meta-analysis of gene expression microarray data
PublikacjaBackground A vast amount of microarray data on transcriptomic response to injury has been collected so far. We designed the analysis in order to identify the genes displaying significant changes in expression after wounding in different organisms and tissues. This meta-analysis is the first study to compare gene expression profiles in response to wounding in as different tissues as heart, liver, skin, bones, and spinal cord, and...
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Human UDP-Glucuronosyltransferases: Effects of altered expression in breast and pancreatic cancer cell lines.
PublikacjaIncreased aerobic glycolysis and de novo lipid biosynthesis are common characteristics of invasive cancers. UDP-glucuronosyltransferases (UGTs) are phase II drug metabolizing enzymes that in normal cells possess the ability to glucuronidate these lipids and speed their excretion; however, de-regulation of these enzymes in cancer cells can lead to an accumulation of bioactive lipids, which further fuels cancer progression. We hypothesize...
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TRNA-Leu (UUR) gene haplotypes observed in canine mammary gland tumours and its deleterious effect assessment according to the comparative analysis with TRNL1 human gene
Dane BadawczeThe aetiology and pathogenesis of many canine tumours are likely to be similar to cancers found in humans. This study aimed to present a plausible link between changes in tRNA-Leu (UUR) gene and the carcinogenesis process in dogs with mammary gland tumours. The whole mitochondrial DNA (mtDNA) isolated from blood and tumour tissues of 13 dogs with malig-nant...
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A study on fibre-reinforced concrete elements properties based on the case of habitat modules in the underwater sills
PublikacjaHydrotechnical constructions are mostly objects functioning in extreme conditions and requiring a custom-made construction project. In the case of using prefabricated elements, it is required to develop production, transport, assembly, conservation and repair technology. Concerning the problem of concrete cracks, modern repair systems allow positive effects to be achieved in many cases of concrete elements repair. In this work...
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Crystal structures of aminotransferases Aro8 and Aro9 from Candida albicans and structural insights into their properties
PublikacjaAminotransferases catalyze reversibly the transamination reaction by a ping-pong bi-bi mechanism with pyridoxal 5′-phosphate (PLP) as a cofactor. Various aminotransferases acting on a range of substrates have been reported. Aromatic transaminases are able to catalyze the transamination reaction with both aromatic and acidic substrates. Two aminotransferases from C. albicans, Aro8p and Aro9p, have been identified recently, exhibiting...
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In Silico Safety Assessment of Bacillus Isolated from Polish Bee Pollen and Bee Bread as Novel Probiotic Candidates
PublikacjaBacillus species isolated from Polish bee pollen (BP) and bee bread (BB) were characterized for in silico probiotic and safety attributes. A probiogenomics approach was used, and in-depth genomic analysis was performed using a wide array of bioinformatics tools to investigate the presence of virulence and antibiotic resistance properties, mobile genetic elements, and secondary metabolites. Functional annotation and Carbohydrate-Active...
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Comparative review of piezoelectric biomaterials approach for bone tissue engineering
PublikacjaBone as a minerals’ reservoir and rigid tissue of the body generating red and white blood cells supports various organs. Although the self-regeneration property of bone, it cannot regenerate spontaneously in severe damages and still remains as a challenging issue. Tissue engineering offers several techniques for regenerating damaged bones, where various biomaterials are examined to fabricate scaffolds for bone repair. Piezoelectric...
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Beta-Galactosidases of Pseudoalteromonas sp. – Characteristics and application for lactose hydrolysis and galactopyranosides synthesis
PublikacjaBeta-galactosidase [EC 3.2.1.23] is an enzyme that catalyzes the hydrolysis of O-glycosidic linkages in galactosides. It has biotechnological applications in the dairy industry for the production of milk with a low content of lactose and galactooligosacharides for use in healthy food. The transgalactosylation activity of beta-galactosidase can be used in the synthesis of alkyl galactopyranosides. Due to the fact that conditions of...
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Peptidoglycan hydrolases - potential weapons against Staphylococcus aureus
PublikacjaBacteria of the genus Staphylococcus are common pathogens responsible for a broad spectrum of human and animal infections and belong to most important etiological factors causing food poisoning. Because of rapid increase in prevalence of isolation of staphylococci resistant to many antibiotics, there is an urgent need for development of new alternative chemotherapeutics. A number of studies have recently demonstrated the strong...
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Inhibiting activity of HIV-1: protease, reverse transcriptase and integrase all together by novel compounds using computational approaches (flexible and rigid docking)
PublikacjaAcquired immunodeficiency syndrome (AIDS), caused by human immunodeficiency virus type 1 (HIV-1) infection, is one of the most challenging diseases in recent decades. Nevertheless the shortcomings of chemical drugs such as toxicity, lack of curative effects, the search for more potent anti-HIV agents have been focused in our study. In current study, novel scaffold was designed having a benzyl and imidazole in it which are very...
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In Situ Random Microseeding and Streak Seeding Used for Growth of Crystals of Cold-Adapted beta-D-Galactosidases: Crystal Structure of betaDG from Arthrobacter sp. 32cB
PublikacjaThere is an increasing demand for cold-adapted enzymes in a wide range of industrial branches. Nevertheless, structural information about them is still scarce. The knowledge of crystal structures is important to understand their mode of action and to design genetically engineered enzymes with enhanced activity. The most difficult task and the limiting step in structural studies of cold-adapted enzymes is their crystallization,...
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Międzyłańcuchowe sieciowanie DNA jako mechanizm biologicznego działania an- tracenodionów.**2003, 100 s. 30 rys. 20 tab. bibliogr. 110 poz. maszyn. Rozprawa doktorska /23.06.2003/ P. Gdań. Wydz. Chem. Promotor: prof. dr hab. inż. J. Konopa.
Publikacja.
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Changes in gene methylation patterns in neonatal murine hearts: Implications for the regenerative potential
PublikacjaBackground The neonatal murine heart is able to regenerate after severe injury; this capacity however, quickly diminishes and it is lost within the first week of life. DNA methylation is an epigenetic mechanism which plays a crucial role in development and gene expression regulation. Under investigation here are the changes in DNA methylation and gene expression patterns which accompany the loss of regenerative potential. Results The...
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Novel therapeutic compound acridine–retrotuftsin action on biological forms of melanoma and neuroblastoma
PublikacjaPURPOSE: As a continuation of our search for anticancer agents, we have synthesized a new acridine-retrotuftsin analog HClx9-[Arg(NO2)-Pro-Lys-Thr-OCH3]-1-nitroacridine (named ART) and have evaluated its activity against melanoma and neuroblastoma lines. Both tumors develop from cells (melanocytes, neurons) of neuroectodermal origin, and both are tumors with high heterogeneity and unsatisfactory susceptibility to chemotherapies....
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Seabed characterisation using spectral moments of the echo signal
PublikacjaZbadano użyteczność zastosowania momentów spektralnych obwiedni echa w rozpoznawaniu i klasyfikacji dna morskiego. Przeprowadzono obliczenia momentów spektralnych dla około 15000 ech od dna zarejestrowanych w rejonie Bałtyku Południowego i potwierdzono tezę, że wartości tych momentów skorelowane są silnie z typem dna i rodzajem osadów.
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Stan przedawaryjny wykopu głębokiego w obudowie z pali CFA
PublikacjaW artykule przedstawiono stan przedawaryjny wykopu głębokiego w obudowie z pali CFA. Omówiono warunki geotechniczne podłoża oraz rozwiązania projektowe. Przeprowadzono analizę dwóch wersji projektów konstrukcyjnych oraz fazę wykonawstwa z wskazaniem na liczne błędy. Przedstawiono możliwe oraz przyjęte działania naprawcze. Omówiono problemy i błędy projektowe na etapie działań naprawczych. Wskazano przyczyny wystąpienia stanu przedawaryjnego.
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Metylotransferazy ssaków – inhibitory i aktywatory
PublikacjaWśród metylotransferaz ssaków możemy wyróżnić metylotransferazy histonowe oraz metylotransferazy DNA. Metylacja DNA w obrębie wysp CpG oraz metylacja histonów wpływają na poziom ekspresji genów w komórkach ssaczych. Dodatkowo dzięki metylacji zapewniana jest stabilność i integralność genomu ludzkiego. Metylotransferazy DNA, będące głównym tematem tego artykułu, są często przedmiotem badań, gdyż nieprawidłowa metylacja DNA jest...
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Chloroacridine derivatives as potential anticancer agents which may act as tricarboxylic acid cycle enzyme inhibitors
PublikacjaThe chloroacridines affect biological forms of melanoma in different ways. Amelanotic (Ab) melanoma (with inhibited melanogenesis and higher malignancy) was particularly sensitive to the action of the chloroacridines. The Ab melanoma cells died through apoptosis and through death without caspase activation. Diminished activity of TAC enzymes was noticed among Ab melanoma cells together with ATP/NAD depletion, especially in the...
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The Application of Dynamic Electrochemical Impedance Spectroscopy (DEIS) Technique in Corrosion and Corrosion Inhibition Studies
Publikacjaorrosion studies have attracted considerable interest in the areas of materials chemistry and industrial chemistry, as it affects the direct and indirect costs of industry, leading to huge economic setbacks due to the need for repair, maintenance, and even shutdowns due corrosion damage. This new volume is a comprehensive resource that presents new and up-to-date, theoretical, and experimental corrosion inhibition studies.
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DDLMS PCR double digestion Ligation Mediated Suppression PCR - a new technique for bacterial specific differentiation
PublikacjaA new diagnostic kit for K. oxytoca specific differentiation based on ddLMS PCR (ang. double digest ligation Mediated PCR) technique is shown. As a species-specific DNA fragment pehX gene, encoding the enzyme polygalactouronase, was chosen. The genome sequence of K. oxytoca is digested with two endonucleases: AclI and BclI which cut DNA before and after pehX gene. The polymorphic DNA fragments are ligated with AclI-end-specific...
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Loop-mediated isothermal amplification (LAMP) as a diagnostic tool in detection of infectious diseases
PublikacjaLoop-mediated isothermal amplification (LAMP) is a gene amplification method which amplifies DNA with high specificity and efficiency under isothermal conditions. Because of its rapidity and simplicity, it is a valuable diagnostic tool in the early detection and identification of infectious diseases. LAMP method is based on the use of a set of four to six specially designed primers spanning six to eight distinct sequences on the...
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Sensitivity of echo parameters to seafloor properties and depth variability.
PublikacjaPrzeprowadzono badanie użyteczności wybranych parametrów echa akustycznego, w szczególności wymiaru fraktalnego oraz momentów spektralnych obwiedni, w klasyfikacji dna morskiego, ze szczególnym uwzględnieniem wpływu zmieniającej się głębokości. Wykorzystano numeryczną symulację rozpraszania echa na dnie oraz innych elementów procesu akustycznego sondowania dna, w tym także procedurę kompensacji wpływu zmian głębokości dna na echo.
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Opracowanie technologii spawania naprawczego szyn poddźwigowych A120 oraz badania nieniszczące jakości szyn w DCT w Porcie Północnym
PublikacjaEkspertyza dotyczy realizacji zlecenia związanego z naprawą spoin szyn poddźwigowych na terenie terminalu kontenerowego - Deep Water Terminal (DCT) w Porcie Północnym w Gdańsku. W ramach opracowania: 1. Wykonano badania ultradźwiękowe (UT) i magnetyczno-proszkowe (MT) spoin czołowych szyn poddźwigowych typu A120, według schematu dostarczonego przez firmę SKANSKA S.A. 2. Wykonano analizę wyników badań...
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Michał Szydłowski dr hab. inż.
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The antioxidative properties of white cabbage (Brassica oleracea var. capitata f. alba) fresh and submitted to culinary processing
PublikacjaKapusta biała stanowi jeden z podstawowych składników diety w Europie centralnej. W publikacji autorzy skupili się na określeniu właściwościach przeciwutleniających kapusty świeżej, kiszonej i poddanej obróbce termicznej. Te właściwości zostały oszacowane dla soków przy wykorzystaniu systemów pozakomórkowych, w komórkach HT29 pod kątem ochrony przed utlenieniem DNA oraz poprzez symulację GSTs i naprawę DNA. Wszystkie badane soki...
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Synthesis of phosphono and phosphate derivatives of hydroxyimino-D-alditols as new potential antifungal agents
PublikacjaIn search for new effective we antyfungals we focus on two enzymes involved in biosynthesis of the fungal cell wall. The first enzyme is glucosamine-6-phosphate (GlmS), which catalyzes transformation of D-fructose-6-phosphate (Fru-6P) to D-glucosamine-6-phodphate (GlcN-6P) in the chitin biosynthesis pathway. The second enzyme is phosphomannose isomerase (PMI) repored to play a crucial role in biosynthesis of many mannosylated structures,...
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Support Materials of Organic and Inorganic Origin as Platforms for Horseradish Peroxidase Immobilization: Comparison Study for High Stability and Activity Recovery
PublikacjaIn the presented study, a variety of hybrid and single nanomaterials of various origins were tested as novel platforms for horseradish peroxidase immobilization. A thorough characterization was performed to establish the suitability of the support materials for immobilization, as well as the activity and stability retention of the biocatalysts, which were analyzed and discussed. The physicochemical characterization of the obtained...
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Telomere uncapping by common oxidative guanine lesions: Insights from atomistic models
PublikacjaOxidative damage to DNA is widely known to contribute to aging and disease. This relationship has been extensively studied for telomeres – structures that cap chromosome ends – due to their role in cell proliferation and senescence, and exceptional susceptibility to oxidation. Indeed, the repetitive telomeric DNA sequence contains the 5′-GGG-3′ motif that has the lowest ionization potential of all trinucleotides. Accordingly, experiments...
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Sensitivity analysis based on non-intrusive regression-based polynomial chaos expansion for surgical mesh modelling
PublikacjaThe modelling of a system containing implants used in ventral hernia repair and human tissue suffers from many uncertainties. Thus, a probabilistic approach is needed. The goal of this study is to define an efficient numerical method to solve non-linear biomechanical models supporting the surgeon in decisions about ventral hernia repair. The model parameters are subject to substantial variability owing to, e.g., abdominal wall...
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The construction of bifunctional fusion proteins consisting of MutS and GFP
PublikacjaSkonstruowano białka chimeryczne zawierające białko MutS z Thermus thermophilus i białko zielonej fluoresceiny GFP z Aequorea victoria, posiadające dla łatwiejszego oczyszczania domeny oligo-histydynowe na N- lub C-końcu. Fuzyjne białka rozpoznawały niekomplementarności w DNA podobnie do MutS T. thermophilus. Fluorescencyjne białka rozpoznające niekomplementarne DNA mogą być użyteczne w wykrywaniu jednonukleotydowych polimorfizmów...
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Elucidating the impact of enzymatic modifications on the structure, properties, and applications of cellulose, chitosan, starch and their derivatives: a review
PublikacjaPolysaccharides, as one of the most prominent natural biopolymers, display numerous biological activities and industrial applications. Nevertheless, some polysaccharides lack biological properties or display weak biological activities. Besides, practical usages of polysaccharides still remain challenging because of their poor solubility and inappropriate hydrophilic/hydrophobic balance. Hence, enzymatic or chemical modifications...