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Wyniki wyszukiwania dla: L-LYSINE BIOSYNTHESIS
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Developments in the synthesis and biological activity of Glycyl-L-Histydyl-L-Lysine derivatives
PublikacjaWe emphasize on the methods of the synthesis described in the literature and present the aspects of Gly-His-Lys structure modifications that played a key role in scientific research.
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Effect of pH on optical sensing with poly-L-lysine-modified nanodiamonds
PublikacjaNitrogen-vacancy (NV) centers are crystallographic defects which provide diamonds with unique physical properties. The centers are known for their intensive, time-stable fluorescence, and an electron spin, which exhibits long coherence time and may be manipulated using external stimuli. Nanodiamonds containing the NV centers are promising tools in biolabeling, biosensing, and drug delivery due to the aforementioned properties of...
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Influence of methyl jasmonate on ginsenoside biosynthesis in suspension cultures of Panax quinquefolium L.
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Fungal L-Methionine Biosynthesis Pathway Enzymes and Their Applications in Various Scientific and Commercial Fields
PublikacjaL-methionine (L-Met) is one of the nine proteinogenic amino acids essential for humans since, in human cells, there are no complete pathways for its biosynthesis from simple precursors. L-Met plays a crucial role in cellular function as it is required for proper protein synthesis, acting as an initiator. Additionally, this amino acid participates in various metabolic processes and serves as a precursor for the synthesis of S-adenosylmethionine...
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Poly-L-Lysine-modified boron-doped diamond electrodes for the amperometric detection of nucleic acid bases
PublikacjaBoron-doped diamond (BDD) is a very promising supporting material used in the construction of biosensors for molecular recognition. The direct immobilization of structurally-organized huge molecules, such as poly-L-Lysine (PLL) provides the possibility of determining organic molecules, e.g. nucleic acid bases (e.g. adenine, guanine) or peptides and proteins. This paper describes the direct method for chemical and electrochemical...
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Enzymes of the L-methionine biosynthesis pathway in Candida albicans as potential novel targets for antifungal chemotherapy
PublikacjaFungal pathways of essential amino acids biosynthesis provide an abundant source of molecular targets for new antifungal compounds, among which the L-methionine biosynthesis pathway (MBP) may be promising. In this dissertation I characterized three C. albicans enzymes involved in MBP: homoserine O-acetyltransferase (CaMet2p), O-acetyl-L-homoserine sulfhydrylase (CaMet15p), and cystathionine-γ-synthase (CaStr2p). I performed crystallization...
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Poly-L-Lysine-functionalized fluorescent diamond particles: pH triggered fluorescence enhancement via surface charge modulation
PublikacjaRecently, the interest in applying fluorescent diamond particles (FDPs) containing nitrogen-vacancy (NV) centers for enhancing the mechanical and chemical properties of some materials, biological imaging, and sensing has been expanding rapidly. The unique properties of NV centers such as intensive, time-stable fluorescence, and an electron spin, which exhibits long coherence time and may be manipulated using external stimuli, such...
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Branched Poly(l-lysine)-Derived Nitrogen-Containing Porous Carbon Flake as the Metal-Free Electrocatalyst toward Efficient Oxygen Reduction Reaction
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- An unique fungal lysine biosynthesis enzymes: new targets for antifungal agents? Grzybowy szlak biosyntezy lizyny: nowy cel molekularny w chemoterapii przeciwgrzybowej?
ProjektyProjekt realizowany w Wydział Chemiczny zgodnie z porozumieniem POMOST/2010-2/4 z dnia 2011-05-17
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Characterization of two aminotransferases from Candida albicans
PublikacjaAminoadipate aminotransferase (AmAA) is an enzyme of α-aminoadipate pathway (AAP) for l-lysine biosynthesis. AmAA may also participated in biosynthesis or degradation of aromatic amino acids and in d-tryptophan based pigment production. The AAP is unique for fungal microorganisms. Enzymes involved in this pathway have specific structures and properties. These features can be used as potential molecular markers. Enzymes catalyzing...
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Enzymes of the lysine biosynthetic pathway as targets for antifungals ?
PublikacjaSystemic infections caused by human pathogenic fungi in immunocompromized patients continue to be one of the important clinical problems. Limited availability of safe and efficacious antifungal chemotherapeutics and emerging resistance to existing drugs stimulates search for novel molecular targets for antifungals. The α-aminoadipate pathway (AAP) of L-lysine biosynthesis is unique in fungi and thus has been so far considered...
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Versatility of putative aromatic aminotransferases from Candida albicans.
PublikacjaAmino acids constitute the key sources of nitrogen for growth of Candida albicans. In order to survive inside the host in different and rapidly changing environments, this fungus must be able to adapt via its expression of genes for amino acid metabolism. We analysed the ARO8, ARO9, YER152C, and BNA3 genes with regards to their role in the nutritional flexibility of C. albicans. CaAro8p is undoubtedly the most versatile enzyme...
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Antifungal Activity of Homoaconitate and Homoisocitrate Analogs
PublikacjaThirteen structural analogs of two initial intermediates of the L-alpha-aminoadipate pathway of L-lysine biosynthesis in fungi have been designed and synthesized, including fluoro- and epoxy-derivatives of homoaconitate and homoisocitrate. Some of the obtainedcompounds exhibited at milimolar range moderate enzyme inhibitory properties against homoaconitase and/or homoisocitrate dehydrogenase of Candida albicans. The structural...
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Phenotypic consequences of the LYS4 gene disruption in Candida albicans
PublikacjaThe main scientific purpose of our studies was to verify the hypothesis that homoaconitase (HA) from Candida albicans, an enzyme catalyzing a second step of the α-aminoadipate pathway (AAP) of L-Lys biosynthesis may become a new target for antifungal chemotherapy. Previous studies indicated that the A. fumigatus mutant lacking the functional lysF gene, encoding HA, exhibited attenuated virulence in a low-dose mouse infection model...
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Consequences of lysine auxotrophy for Candida albicans adherence and biofilm formation
PublikacjaA number of factors are known to be involved in Candida albicans virulence, although biofilm development on the surfaces of indwelling medical devices is considered to promote superficial or systemic disease. Based on previously reported up-regulation of saccharopine and acetyllysine in biofilm cells and activation of the lysine biosynthesis/degradation pathway, we investigated...
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Changes in Epigenetic Patterns Related to DNA Replication in Vicia faba Root Meristem Cells under Cadmium-Induced Stress Conditions
PublikacjaExperiments on Vicia faba root meristem cells exposed to 150 µM cadmium chloride (CdCl2) were undertaken to analyse epigenetic changes, mainly with respect to DNA replication stress. Histone modifications examined by means of immunofluorescence labeling included: (1) acetylation of histone H3 on lysine 56 (H3K56Ac), involved in transcription, S phase, and response to DNA damage during DNA biosynthesis; (2) dimethylation of histone...
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Plasmids pMET2M1 and pMET15M1 with the SAT1 Flipper knockout cassette construction
Dane BadawczeThe goal of the study was to obtain plasmids containing the SAT1 Flipper knockout cassette along with upstream and downstream gene fragments (MET2, MET15) and to introduce a cleavage site for restriction enzymes into them.
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Inserts amplification for knockout cassette construction
Dane BadawczeThe goal of the study was to obtain optimal conditions for amplification of upstream and downstream genes fragments and introduce a cleavage site for restriction enzymes into them thanks to which it will be possible to clone fragments into a plasmid containing elements of the knockout cassette. The constructed cassettes will enable the removal of the...
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Novel application of terminal restriction fragments length polymorphism method (t-RFLP) for identification of six clinically important Candida sp.
PublikacjaTerminal restriction fragments length polymorphism method (t-RFLP) is a modified classical RFLP method. The main difference is based on carrying out PCR using a fluorescently labeled primer or primers. The result of such modification is that after digestion of amplicons only terminal fragments of PCR product (containing fluorescent dye) are visualized. T-RFLP method has found application in environmental microbiology and epidemiology....
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Heptaene macrolides biosynthesis by Streptomyces species
PublikacjaPolyene macrolides are one of the groups of secondary metabolites, generated by microorganisms belonging to the Streptomyces genus. These compounds, containing 3 - 7 conjugated double bonds systems in their molecules exhibit high antifungal activity against a broad spectrum of fungal pathogens with heptaenes demonstrating the highest antifungal potential. At the large extent, efficiency of biosynthesis of these natural products...
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Inhibitors of amino acids biosynthesis as antifungal agents
PublikacjaFungal microorganisms, including the human pathogenic yeast and filamentous fungi, are able to synthesize all proteinogenic amino acids, including nine that are essential for humans. A number of enzymes catalyzing particular steps of human-essential amino acid biosynthesis are fungi specific. Numerous studies have shown that auxotrophic mutants of human pathogenic fungi impaired in biosynthesis of particular amino acids exhibit...
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Lipopolysaccharides: regulated biosynthesis and structural diversity
PublikacjaThe cell envelope of Gram-negative bacteria contains two distinct membranes, an inner (IM) and an outer (OM) membrane, separated by the periplasm, a hydrophilic compartment that includes a thin layer of peptidoglycan. The most distinguishing feature of such bacteria is the presence of an asymmetric OM with phospholipids located in the inner leaflet and lipopolysaccharides (LPSs) facing the outer leaflet. The maintenance of this...
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The bioactivity of processed garlic (Allium sativum L.) as shown in vitro and in vivo studies on rats
PublikacjaCzosnek w trakcie procesu obróbki traci znaczną część swojej bioaktywności. Właściwości przeciwutleniające zmierzone metodą FRAP i DPPH stwierdzono na najwyższym poziomie w przypadku czosnku surowego oraz po krótkim okresie gotowania. Badania na szczurach poddanych diecie z dodatkiem krótkogotowanego czosnku potwierdziły, że proces ten nie zaburza właściwości przeciwutleniających surowego czosnku. U zwierząt stwierdzono poprawę...
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Targeting Candida albicans O-acetyl-L-homoserine sulfhydrylase (Met15p) in antifungal treatment
PublikacjaFungal infections are a serious threat to public health as they are becoming increasingly frequent. A major problem stems also from a rising fungal resistance to currently available antifungal therapies, therefore novel molecular targets are highly desirable. Exploration of enzymes participating in the biosynthesis pathways of essential amino acids such as L-methionine (L-Met) may provide new insights into pharmaceutical development....
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Suppressors of lapC mutation identify new regulators of LpxC, which mediates the first committed step in lipopolysaccharide biosynthesis
PublikacjaGram-negative bacteria, such as Escherichia coli, are characterized by an asymmetric outer membrane (OM) with lipopolysaccharide (LPS) located in the outer leaflet and phospholipids facing the inner leaflet. E. coli recruits LPS assembly proteins LapB, LapC and LapD in concert with FtsH protease to ensure a balanced biosynthesis of LPS and phospholipids. We recently reported that bacteria either lacking the periplasmic domain of...
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Stability of oil/water (ethanol, lysozyme or lysine) emulsions
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Checkpoints that regulate balanced biosynthesis of lipopolysaccharide and its essentiality in Escherichia coli
PublikacjaThe outer membrane (OM) of Gram-negative bacteria, such as Escherichia coli, is essential for their viability. Lipopolysaccharide (LPS) constitutes the major component of OM, providing the permeability barrier, and a tight balance exists between LPS and phospholipids amounts as both of these essential components use a common metabolic precursor. Hence, checkpoints are in place, right from the regulation of the first committed step...
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Enzymes of UDP-GlcNAc biosynthesis in yeast
PublikacjaD-glukozamina stanowi ważny składnik budulcowy głównych elementów strukturalnych grzybowej ściany komórkowej: chityny, chitozanu i mannoprotein. Inne aminocukry, takie jak D-mannozamina i D-galaktozamina, stosunkowo powszechne w komórkach wyższych organizmów eukariotycznych, rzadko występują u grzybów i są praktycznie nieobecne w komórkach drożdży i drożdżaków. Zawierający glukozaminę nukleotyd cukrowy UDP-GlcNAc jest syntezowany...
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Three-dimensional Weyl topology in one-dimensional photonic structures
PublikacjaTopological features, in particular distinct band intersections known as nodal rings, usually requiring three-dimensional structures, have now been demonstrated experimentally in an elegantly simple one-dimensional photonic crystal.
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Modulation of TET2 Activity By Ascorbic Acid and Factors Affecting Lysine Acetylation
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Synthesis of linear tuftsin analogues modified at the epsilon-amino group of lysine
PublikacjaZsyntetyzowano na fazie stałej nowe analogi tuftsyny i retro-tuftsyny zawierające wiązanie isopeptydowe na epsilon-aminowej grupie lizyny jako potencjalne immunostymulatory. Do syntezy wykorzystano standardową procedurę Fmoc. Po zdjęciu osłon kwasem TFA końcowe związki były oczyszczane, liofilizowane i charakteryzowane: MS, HPLC i NMR.
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The application of Monod equation to denitrification kinetics description in the moving bed biofilm reactor (MBBR)
PublikacjaIn this paper, the kinetic constants Vmax and KCOD occurring in the Monod equation, which describe the denitrification process in the moving bed, are determined. For this purpose, a laboratory moving bed biofilm reactor (MBBR) was used. The filling of the reactor consisted of EvU Perl carriers. The experiment was carried out with an excess of nitrate, and denitrification rate was dependent on the concentration of external organic...
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Continuous three-dimensional radiation dosimetry in tissue-equivalent phantoms using electron paramagnetic resonance in L -α-alanine
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Dynamics of ginsenoside biosynthesis in suspension culture of Panax quinquefolium
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Biosynthesis of methanol from methane by Methylosinus trichosporium OB3b
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Phenotypic and genotypic identification of atypical L. monocytogenes
Dane BadawczeThe data represent the results of research within the Miniatura 5 scientific activity entitled "Characterization of atypical Listeria monocytogenes strains isolated from the food production environment financed by the National Science Center (no. DEC 2021/05/X/NZ9/00143). The aim of this part of the project was (i) to learn about the differences between...
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Lipid oxidation and lysine availability in Atlantic mackerel hot smoked in mild conditions.
PublikacjaZawartość nadtlenków w wędzonych makrelach była o 50% mniejsza, a liczba anizydynowa 2,5 razy większa niż w surowych rybach. Przechowywanie ryb wędzonych w temp. +2C nie wpłynęło na zawartość produktów utlenienia lipidów. Zawartość dostępnej lizyny była podobna w surowych i wędzonych rybach. Skóra ryb wędzonych zawierała 2 razy więcej fenoli niż tkanka mięśniowa.
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Lipid oxidation and lysine availability in smoked Atlantic mackerel fillets and Baltic sprats.
PublikacjaNie wykazano korelacji między stopniem utlenienia lipidów a ich zawartością w rozmrożonych filetach makreli. Gorące i zimne wędzenie nie wływało na liczbę nadtlenkową w mięsie filetów makreli, natomiast w mięsie wędzonych szprotów była ona znacznie mniejsza niż w rybach surowych. Wędzenie nie powodowało obniżenia dostępnej lizyny, chociaż liczba anizydynowa zwiększyła się dwukrotnie w wędzonych filetach makreli i czterokrotnie...
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Estampille (L’) / Objet d'art (L’)
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Evaluation of Selected Heavy Metal Contaminants as Well as Nitrates and Nitrites in the Microgreens of Nigella (Nigella sativa L.), Safflower (Carthamus tinctorius L.), and Camelina (Camelina sativa L.) at Different Stages of Vegetation
PublikacjaMicrogreens are a new, rapidly growing group of foodstuffs. The decorative function of these is often accompanied by their use in traditional dishes. As microgreens are eaten at very early stages, when the development of the epidermis is at its minimum, the bioavailability of minerals will be found to be higher in microgreens then in mature vegetables. So, microgreens can be an excellent functional food, especially for mineral-deficient...
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Anthropologie (L')
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Syntheses and crystal structures of lithium derivatives of diphosphanes R2P-P(SiMe3)Li·3L, R = Ph, iPr and iPr2N, L = THF or DME
PublikacjaW wyniku reakcji R2P-P(SiMe3)2 z BuLi w THF lub DME powstają pochodne litowe difosfanów R2P-P(SiMe3)Li·3L. Dla R = iPr oraz iPr2N powstają sole R2P-P(SiMe3)Li·3THF, w których atom litu jest czterokrotnie koordynowany a dla R = Ph powstaje sól [Li·3DME]+[Ph2P-PSiMe3]-, która posiada budowę jonową. W reakcji BuLi z Ph2P-P(SiMe3)2 powstają znaczne ilości produktów ubocznych: Ph2P-PPh2 i LiP(SiMe3)2.
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KONSTRUKCJA AUKSOTROFICZNEGO WZGLĘDEM L-LIZYNY SZCZEPU CANDIDA ALBICANS
PublikacjaEnzymy szlaku kwasu L-α-aminoadypinowego biosyntezy L-lizyny z Candida albicans są potencjalnymi celami molekularnymi w terapii przeciwgrzybowej. Niektóre grupy badawcze wykazały, że auksotrofia wobec L-lizyny u pewnych gatunków grzybów powoduje zmniejszenie ich zjadliwości i może mieć wpływ na wzrost in vivo [Liebmann i in. 2004]. Oznacza to, że można się spodziewać zahamowania wzrostu komórek grzybowych w wyniku działania selektywnych...
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Harnessing endophytic fungi for biosynthesis of selenium nanoparticles and exploring their bioactivities
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Identification of L. monocytogenes and atypical isolates by MALDI TOF MS.
Dane BadawczeThe data represent the results of research within the scientific activity Miniatura 5 entitled "Characterization of atypical Listeria monocytogenes strains isolated from the food production environment financed by the National Science Center (no. DEC 2021/05/X/NZ9/00143). The aim of this part of the project was (i) to determine the peptide composition...
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Synthesis, Pharmacological Evaluation, and Computational Studies of Cyclic Opioid Peptidomimetics Containing β3-Lysine
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Double ZIF-L structures with exceptional CO2 capacity
PublikacjaCarbon dioxide emission is an emerging problem nowadays and new methods are designed for its control. In this article, a report on the formation of zeolitic imidazole framework with an apparent double leaf-like morphology DZIF-L is given. The special structure of the materials prevents from aggregation of the particles providing remarkable CO2 capacity. At 0.1 MPa the CO2 uptake was 2.99 ± 0.06 mmol/g. The capacity of synthesized...
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Investigation of the Effect of the Rocket's Suborbital Flight on Biofilm, Enzymes and Biosynthesis on Autonomous, Modular and Scalable Platforms For Conducting Experiments of an Astrobiotechnological Nature
PublikacjaWith new incentives for human space exploration, biotechnological experiments in orbit became imperative. Answering this need, we build an autonomous, modular, and scalable platform that enables those experiments on rockets. We called it AMBER. The aim of the payload of the R6 suborbital rocket is to perform an experiment to study the influence of rocket flights on biofilm, molecular biology enzymes and biosynthesis using our platform....
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EEG-Based Analysis of ASMR Stimuli: A Pilot Study of Neuropsychological Responses through Conventional vs. Bone-Conduction Headphones
PublikacjaIn this study, the impact of ASMR (Autonomous Sensory Meridian Response) experiences delivered through different types of headphones was evaluated with respect to neural responses and anxiety levels. The EEG data of a 24-year-old participant was recorded while he underwent ASMR stimulation using conventional and bone-conduction headphones. The State-Trait Anxiety Inventory (STAI) assessed anxiety levels before and after ASMR stimulation,...
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Absorbing Boundary Conditions Derived Based on Pauli Matrices Algebra
PublikacjaIn this letter, we demonstrate that a set of absorbing boundary conditions (ABCs) for numerical simulations of waves, proposed originally by Engquist and Majda and later generalized by Trefethen and Halpern, can alternatively be derived with the use of Pauli matrices algebra. Hence a novel approach to the derivation of one-way wave equations in electromagnetics is proposed. That is, the classical wave equation can be factorized...