Filtry
wszystkich: 3722
wybranych: 673
Wyniki wyszukiwania dla: AUTOIMMUNE DISEASES
-
Vitamin K status in cystic fibrosis patients with liver cirrhosis
Publikacja -
An innovative panel to assess endothelial integrity of pedicled and skeletonized internal thoracic artery used as aortocoronary bypass graft: a randomized comparative histologic and immunohistochemical study
Publikacja -
Imidazoacridinone-dependent lysosomal photodestruction: a pharmacological Trojan horse approach to eradicate multidrug-resistant cancers
PublikacjaMultidrug resistance (MDR) remains a primary hindrance to curative cancer therapy. Thus, introduction of novel strategies to overcome MDR is of paramount therapeutic significance. Sequestration of chemotherapeutics in lysosomes is an established mechanism of drug resistance. Here, we show that MDR cells display a marked increase in lysosome number. We further demonstrate that imidazoacridinones (IAs), which are cytotoxic fluorochromes,...
-
Teloxantron inhibits the processivity of telomerase with preferential DNA damage on telomeres
PublikacjaTelomerase reactivation is one of the hallmarks of cancer, which plays an important role in cellular immortalization and the development and progression of the tumor. Chemical telomerase inhibitors have been shown to trigger replicative senescence and apoptotic cell death both in vitro and in vivo. Due to its upregulation in various cancers, telomerase is considered a potential target in cancer therapy. In this study, we identified...
-
A novel method of Mycobacterium tuberculosis complex strain differentiation using polymorphic GC-rich gene sequences.
PublikacjaTuberculosis is one of the leading infectious diseases. In this work, a new genotyping method of Mycobacterium tuberculosis (Mtb) complex strain is presented. 27 Mtb genomes were analyzed for the presence of length polymorphism within polymorphic GC-rich gene sequences. Four genes, Rv3345c, Rv3507, Rv0747 and Rv3511, showing variation in length depending on the Mtb strain were selected for designing primer sequences flanking variable...
-
Synthesis and Cholinesterase Inhibitory Activity of N-Phosphorylated /N-Tiophosphorylated Tacrine
PublikacjaNovel phosphorus and thiophosphorus tacrine derivatives were designed, synthesized and their biological activity and molecular modeling was investigated as a new potential anti-Alzheimer's disease (AD) agents. All new synthesized compound exhibited lower toxicity against neuroblastoma cell line (SH-SY5Y) in comparison with tacrine. Two analogues in the series, 7 and 9, demonstrated lack of cytotoxicity against hepatocellular cells...
-
Non-Nutritive Bioactive Compounds in Food of Plant Origin
PublikacjaThe increasing knowledge on the health benefits of certain food ingredients, in particular, those of plant origin, opened the discussion of the possibility of using edible plants or their active components in the prevention of non-communicable diseases. The health-promoting properties of plant foods are related to the presence of non-nutritive compounds, mainly plant secondary metabolites, which can affect many biological mechanisms...
-
Optical glyphs based localization and identification system
PublikacjaThe paper presents a description of functioning of a platform supporting the detection of obstructive diseases in the respiratory system education process. A 16-parameter model of the respiratory system simulated in the MATLAB/Simulink environment was set in the role of the tested patient. It has been linked to the control layer, developed in the LabVIEW environment, using the SIT library (Simulation Interface Toolkit). This layer...
-
ANALYZING TITLES OF ECONOMY NEWS TO UNDERSTAND IMPACT OF COVID-19 ON ECONOMICAL SITUATION
PublikacjaCovid-19 affected the whole world in a short time, causing serious panic and uncertainty in society. Because it was an unprecedented disease, the medical community has worked hard to find out how to deal with it, and it continues to do so. The rapid spread of the disease, the shortage of hospital capacity and the increase in deaths drove the whole world to a closure, so to speak. In this time period, life in the world came to a...
-
Classification of Covid-19 using Differential Evolution Chaotic Whale Optimization based Convolutional Neural Network
PublikacjaCOVID-19, also known as the Coronavirus disease-2019, is an transferrable disease that spreads rapidly, affecting countless individuals and leading to fatalities in this worldwide pandemic. The precise and swift detection of COVID-19 plays a crucial role in managing the pandemic's dissemination. Additionally, it is necessary to recognize COVID-19 quickly and accurately by investigating chest x-ray images. This paper proposed a...
-
Application of the laser diode with central wavelength 975 nm for the therapy of neurofibroma and hemangiomas
PublikacjaThis paper presents newly developed dermatological laser (with central wavelength 975 nm) for application in therapies requiring deep penetration of tissue, e.g. cutaneous (dermal) neurofibroma (Recklinghausen disease) and hemangiomas. This laser can work either in pulse or continues wave mode. Laser radiation is transmitted toward the application region by optical fiber with a diameter of 0.6 mm. The compact design of the laser...
-
Does pandemic denial help or harm? Belief in COVID-19 and experience of it affect psychological consequences of using neurotic defence mechanisms
PublikacjaObjective: The COVID-19 pandemic may activate thoughts of death, leading to aversive psychological states. In such situations, defence mechanisms emerge; however, their adaptability depends on many factors. The aim of the study was to investigate the relationship between neurotic defence style and depressive symptoms. We assumed that the sense of coherence underlies this correlation and investigated how both believing (or not)...
-
Identification of evolutionary conserved DNA sequence and corresponding S21 ribosomal protein region for diagnostic purposes of all Borrelia spirochetes
PublikacjaIt is still under investigation, whether all Borrelia sp. causing Lyme borreliosis and other diseases are already identified and properly classified as human pathogens. For this reason, it is of great importance to develop a diagnostic ELISA test that detects all Borrelia sp. The aim of this study was to identify conserved DNA and protein regions present in all currently known Borrelia sp. In experimental studies 31 available Borrelia...
-
A Measurement System for Quasi-Spectral Determination of Absorption and Scattering Parameters of Veterinary Tissue Phantoms
PublikacjaThis paper describes the construction of a system for the quasi-spectral determination of absorption and scattering parameters of animal tissue phantoms. Several tissue phantoms, including one reference and two modified for examination, were prepared from polydimethylsiloxane (PDMS). The phantoms were measured using a system based on an integrating sphere and the light sources of wavelengths commonly used for the treatment of various...
-
Analysis of the influence of external conditions on temperature readings in thermograms and adaptive adjustment of the measured temperature value
PublikacjaMeasuring human temperature is a crucial step in preventing the spread of diseases such as COVID-19. For the proper operation of an automatic body temperature measurement system throughout the year, it is necessary to consider outdoor conditions. In this paper, the effect of atmospheric factors on facial temperature readings using infrared thermography is investigated. A thorough analysis of the variation of facial temperature...
-
Evaluation of propagation parameters of open guiding structures with the use of complex root finding algorithms
PublikacjaAn efficient complex root tracing algorithm is utilized for the investigation of electromagnetic wave propagation in open guiding structures. The dispersion characteristics of propagated and leaky waves are calculated for a couple of chosen waveguides. The efficiency of the root tracing algorithm is discuses and compared to a global root finding algorithm.
-
Deformation of the Structural Details of the Components Created by the Rapid Prototyping Method
PublikacjaThe paper present the results of preliminary research and describes the deformation of construction details within the process of producing small element employing a stereography system. The article discuses a scheme for changes in data conversion. Variation in construction details have been described on the basis of analysing photographs.
-
Systemic oxidoreductive balance and vascular function in individuals without clinical manifestation of atherosclerosis
Publikacja -
Isolation and identification of Mycobacterium avium subspecies silvaticum from a horse
PublikacjaPo raz pierwszy opracowano metodę identyfikacji Mycobacterium avium subsp. silvaticum na podstawie analizy sekwencji DNA alfa antygenu. Zastosowanie enzymu HhaI do trawienia produktu PCR z uzyskanego z udziałem starterów specyficznych wobec genu kodującego alfa antygen pozwoliło na identyfikację Mycobacterium avium subsp. silvaticum. Opracowana nowa metoda pozwala na prowadzenie badań epidemiologicznych bezpośrednio z próbek pochodzących...
-
WYKORZYSTANIE SIECI NEURONOWYCH I METODY WEKTORÓW NOŚNYCH SVM W PROCESIE ROZPOZNAWANIA AKTYWNOŚCI RUCHOWEJ PACJENTÓW DOTKNIĘTYCH CHOROBĄ PARKINSONA
PublikacjaChoroba Parkinsona (ang. PD - Parkinson Disease) zaliczana jest do grupy chorób neurodegeneracyjnych. Jest to powoli postępująca choroba zwyrodnieniowa ośrodkowego układu nerwowego. Jej powstawanie związane jest z zaburzeniem produkcji dopaminy przez komórki nerwowe mózgu. Choroba manifestuje się zaburzeniami ruchowymi. Przyczyna występowania tego typu zaburzeń nie została do końca wyjaśniona. Leczenie osób dotkniętych PD oparte...
-
A Novel Approach to the Assessment of Cough Incidence
PublikacjaIn this paper we consider the problem of identication of cough events in patients suffering from chronic respiratory diseases. The information about frequency of cough events is necessary to medical treatment. The proposed approach is based on bidirectional processing of a measured vibration signal - cough events are localized by combining the results of forward-time and backward-time analysis. The signal is at rst transformed...
-
The properties, functions and use of selenium compounds in living organisms
PublikacjaSelenium occurs in the environment in inorganic and organic compounds. For many years it was regarded as a toxic element, causing numerous illnesses and diseases. But research in the last 50 years has revealed a "bright side" to this element, especially as a component of selenoproteins, selenium makes a significant contribution to the health of humans and animals. The selenium content in an organism depends on its concentration...
-
Reducing nighttime light exposure in the urban environment to benefit human health and society
PublikacjaNocturnal light pollution can have profound effects on humans and other organisms. Recent research indicates that nighttime outdoor lighting is increasing rapidly. Evidence from controlled laboratory studies demonstrates that nocturnal light exposure can strain the visual system, disrupt circadian physiology, suppress melatonin secretion, and impair sleep. There is a growing body of work pointing to adverse effects of outdoor lighting...
-
Fibrillar aggregates in powdered milk
PublikacjaThis research paper addresses the hypothesis that powdered milk may contain amyloid fibrils. Amyloids are fibrillar aggregates of proteins. Up to this time, research on the presence of amyloids in food products are scarce. To check the hypothesis we performed thioflavin T fluorescence assay, X-ray powder diffraction, atomic force microscopy and fluorescence microscopy imaging. Our preliminary results show that commercially available...
-
Bioactivities of Phenolic Compounds from Kiwifruit and Persimmon
PublikacjaFruit used in the common human diet in general, and kiwifruit and persimmon particularly, displays health properties in the prevention of heart disease. This study describes a combination of bioactivity, multivariate data analyses and fluorescence measurements for the differentiating of kiwifruit and persimmon, their quenching and antioxidant properties. The metabolic differences are shown, as well in the results of bioactivities...
-
Novel monovalent and multivalent recombinant proteins of Borrelia burgdorferi sensu lato with potential diagnostic value – construction and biotechnological production
PublikacjaThe diverse antigenic structure of Borrelia burgdorferi sensu lato (s.l.) and the low degree of protein sequence conservation between genospecies causes many limitations in serodiagnosis of Lyme disease (LD). Using expression systems based on Escherichia coli, five monovalent B. burgdorferi s.l. recombinant proteins were produced. i.e., BB0108, BB0126, BB0298, BB0323, BB0689 (each in three variants derived from Borrelia afzelii,...
-
Deep CNN based decision support system for detection and assessing the stage of diabetic retinopathy
PublikacjaThe diabetic retinopathy is a disease caused by long-standing diabetes. Lack of effective treatment can lead to vision impairment and even irreversible blindness. The disease can be diagnosed by examining digital color fundus photographs of retina. In this paper we propose deep learning approach to automated diabetic retinopathy screening. Deep convolutional neural networks (CNN) - the most popular kind of deep learning algorithms...
-
Nanoparticles: Taking a Unique Position in Medicine
PublikacjaThe human nature of curiosity, wonder, and ingenuity date back to the age of humankind. In parallel with our history of civilization, interest in scientific approaches to unravel mechanisms underlying natural phenomena has been developing. Recent years have witnessed unprecedented growth in research in the area of pharmaceuticals and medicine. The optimism that nanotechnology (NT) applied to medicine and drugs is taking serious...
-
Genome-Wide DNA Methylation and Gene Expression in Patients with Indolent Systemic Mastocytosis
PublikacjaMastocytosis is a clinically heterogenous, usually acquired disease of the mast cells with a survival time that depends on the time of onset. It ranges from skin-limited to systemic disease, including indolent and more aggressive variants. The presence of the oncogenic KIT p. D816V gene somatic mutation is a crucial element in the pathogenesis. However, further epigenetic regulation may also affect the expression of genes that...
-
Streptococcus intermedius Acute Meningitis in an Immunocompetent Child
Publikacja -
The Influence of SARS-CoV-2 Variants B.1.1.7 and B.1.617.2 on a Different Clinical Course and Severity of COVID-19 in Children Hospitalized in 2021 Compared With 2020
Publikacja -
An increased skin microvessel density is associated with neurovascular complications in type 1 diabetes mellitus
Publikacja -
A novel chemiluminescent immunoassay based on original acridinium ester labels as better solution for diagnosis of human toxoplasmosis than conventional ELISA test
PublikacjaToxoplasma gondii infection is one of the most common human zoonosis. Laboratory diagnosis of this disease is mainly based on the results of serological methods detecting specific antibodies in the patient’s sera. In this study we aimed to evaluate the performance of a chemiluminescence immunoassay (CLIA) based on the use of a novel immunochemical reagent in the form of the conjugate of original acridinium label (AL) attached to...
-
A new human IgG avidity test, using mixtures of recombinant antigens (rROP1, rSAG2, rGRA6), for the diagnosis of difficult-to-identify phases of toxoplasmosis
PublikacjaThe preliminary diagnostic utility of two mixtures of Toxoplasma gondii recombinant antigens (rROP1+rSAG2 and rROP1+rGRA6) in IgG ELISA and IgG avidity test has been evaluated. A total of 173 serum samples from patients with toxoplasmosis and seronegative people were examined. The sensitivity of IgG ELISA for rROP1+rSAG2 and rROP1+rGRA6 was 91.1% and 76.7%, respectively, while the reactivity for sera from patients where acute toxoplasmosis...
-
New recombinant chimeric antigens, P35-MAG1, MIC1-ROP1, and MAG1-ROP1, for the serodiagnosis of human toxoplasmosis
PublikacjaThe aim of the study was to evaluate the usefulness of 3 chimeric Toxoplasma gondii antigens, P35-MAG1,MIC1-ROP1 andMAG1-ROP1, in the serodiagnosis of an acute toxoplasmosis in humans. Proteins were produced as fusion proteins containing His tags ends and then further purified by metal affinity chromatography. Their application for the diagnosis of recently acquired T. gondii infection was tested in IgG and IgM enzyme-linked immunosorbent...
-
A novel chemiluminescent immunoassay for detection of Toxoplasma gondii IgG in human sera
PublikacjaThis study describes Toxoplasma gondii IgG chemiluminescent immunoassay (CLIA) based on the use of a novel immunochemical reagents in the form of the conjugates of original acridinium ester (AE) labels attached to antibodies and SAG2-GRA1-ROP1L chimeric antigen and shows that this test is useful for diagnostic purposes.
-
Evaluation of multiple genetic markers for typing drug-resistant Mycobacterium tuberculosis strains from Poland
PublikacjaW publikacji przedstawiono wyniki genotypowania wielolekoopornych szczepów Mycobacterium tuberculosis izolowanych od pacjentów z Polski.
-
Determination of antibiotic residues in honey
PublikacjaHoney produced by honeybees is a valuable food product. The presence of xenobiotics in honey may harm its quality and constitute a danger to human health. Antibiotics are commonly applied by beekeepers to eliminate disease among honeybees. Moreover, ubiquitous administration of antibiotics may cause bacteria to become resistant to many drugs and spread antibioticresistant strains of bacteria. Appropriate sample preparation and...
-
Spirometry measurement model - the diagnostic purpose support
Publikacjathe paper presents a new model of respiratory mechanism based on the spirometry measurements. the spirometry test assesses the efficiency of the lung ventilation. the respiratory system functioning is based on the ventilation mechanism. thus the quality of the lung depends on the quality of lung ventilation. modelling of the respiratory system supports a diagnostic process. the model parameter estimates are obtained on the basis...
-
Milky Sap of Greater Celandine (Chelidonium majus L.) and Anti-Viral Properties
PublikacjaThe milky juice of the greater celandine herb has been used in folk medicine and in homeopathy for treatment of viral warts for years. However, classical medicine fails to use properties of celandine herbs in treatment of diseases induced by papilloma viruses. Nevertheless, dermatological outpatient clinics are regularly visited by patients reporting efficacy of milky sap isolated from celandine herb in treatment of their own...
-
Assessing the Risk in Urban Public Transport for Epidemiologic Factors
PublikacjaPandemics have presented new challenges for public transport organisers and operators. New diseases (e.g., influenza H1N1, severe acute respiratory syndrome—SARS, as well as, more recently, SARS-CoV-2) increase the need for new protection measures to prevent epidemic outbreaks in public transport infrastructure. The authors’ goal is to present a set of actions in the area of public transport that are adjusted to different levels...
-
Detection, identification and determination of resveratrol in wine. Problems and challenges
PublikacjaResveratrol is a compound synthesized by plants in response to unfavorable conditions of growth like mechanical injury, siege of bacteria or fungi and UV exposure. It is concentrated mostly in grapes skin and further more in the products made from grapes especially red wines. Each wine is characterized by different amount of given stilbene since its occurrence is affected by many factors like: types of grapes, environment (climate,...
-
Evaluation of the Electronic Nose Used for Monitoring Environmental Pollution
PublikacjaAir pollution is a one of the major concern of civilized world, which has a significant impact on human health and the environment. Recent studies highlight that the exposure to polluted air can increase the incidence of diseases and deteriorate the quality of life. Hence, it is necessary to develop tools for real-time air quality monitoring. Electronic-nose systems based on sensors are an interesting and promising technology in...
-
Indoor air quality in public utility environments-a review
PublikacjaIndoor air quality has been the object of interest for scientists and specialists from the fields of science such as chemistry, medicine and ventilation system design. This results from a considerable number of potential factors, which may influence the quality of the broadly understood indoor air in a negative way. Poor quality of indoor air in various types of public utility buildings may significantly affect an increase in the...
-
Inhibition of amyloid fibril formation of hen egg white lysozyme by trimethylamine N-oxide at low pH
PublikacjaIn vitro inhibition of the formation of fibrous aggregates of proteins (amyloids) has gained increasing attention due to the number of diseases associated with protein misfolding and fibrillation. An interesting group of compounds for which pronounced activity against this phenomenon can be expected consists of low molecular weight substances (osmolytes) which have the ability to change protein stability. Here we investigate the...
-
Recent advances in aqueous virus removal technologies
PublikacjaThe COVID-19 outbreak has triggered a massive research, but still urgent detection and treatment of this virus seems a public concern. The spread of viruses in aqueous environments underlined efficient virus treatment processes as a hot challenge. This review critically and comprehensively enables identifying and classifying advanced biochemical, membrane-based and disinfection processes for effective treatment of virus-contaminated...
-
Optical method supported by machine learning for dynamics of C‐reactive protein concentrations changes detection in biological matrix samples
PublikacjaIn this article we present the novel spectroscopy method supported with machine learning for real-time detection of infectious agents in wastewater. In the case of infectious diseases, wastewater monitoring can be used to detect the presence of inflammation biomarkers, such as the proposed C-reactive protein, for monitoring inflammatory conditions and mass screening during epidemics for early detection in communities of concern,...
-
Isoprostanes-Biomarkers of Lipid Peroxidation: Their Utility in Evaluating Oxidative Stress and Analysis
PublikacjaIsoprostanes (IsoPs) are key biomarkers for investigating the role of free radical generation in the pathogenesis of human disorders. To solve IsoPs-related problems with regard to isoprostanes, analytical tools are required. This paper reviews the problems and trends in this field focusing on the methodology for assaying biomarkers in exhaled breath condensate (EBC) samples. A large amount of work has been done in the qualitative...
-
Polylysine for Skin Regeneration: A Review of Recent Advances and Perspectives
PublikacjaThere have been several attempts to find promising biomaterials for skin regeneration, among which polylysine (a homopolypeptide) has shown benefits in the regeneration and treatment of skin disorders. This class of biomaterials has shown exceptional abilities due to their macromolecular structure. Polylysine-based biomaterials can not only be used as tissue engineering scaffolds for skin regeneration, but also as drug carriers...
-
CyberEye: New Eye-Tracking Interfaces for Assessment and Modulation of Cognitive Functions beyond the Brain
PublikacjaThe emergence of innovative neurotechnologies in global brain projects has accelerated research and clinical applications of BCIs beyond sensory and motor functions. Both invasive and noninvasive sensors are developed to interface with cognitive functions engaged in thinking, communication, or remembering. The detection of eye movements by a camera offers a particularly attractive external sensor for computer interfaces to monitor,...