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Search results for: F1-ATPASE
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Torsional elasticity and energetics of F1-ATPase
PublicationFoF1-ATPase is a rotary motor protein synthesizing ATP from ADP driven by a cross-membrane proton gradient. The proton flow through the membrane-embedded Fo generates the rotary torque that drives the rotation of the asymmetric shaft of F1. Mechanical energy of the rotating shaft is used by the F1 catalytic subunit to synthesize ATP. It was suggested that elastic power transmission with transient storage of energy in some compliant...
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Rotation Triggers Nucleotide-Independent Conformational Transition of the Empty β Subunit of F1-ATPase
PublicationF1-ATPase (F1) is the catalytic portion of ATP synthase, a rotary motor protein that couples proton gradients to ATP synthesis. Driven by a proton flux, the F1 asymmetric γ subunit undergoes a stepwise rotation inside the α3β3 headpiece and causes the β subunits’ binding sites to cycle between states of different affinity for nucleotides. These concerted transitions drive the synthesis of ATP from ADP and phosphate. Here, we study...
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Mechanochemical Energy Transduction during the Main Rotary Step in the Synthesis Cycle of F1-ATPase
PublicationF1-ATPase is a highly efficient molecular motor that can synthesize ATP driven by a mechanical torque. Its ability to function reversibly in either direction requires tight mechanochemical coupling between the catalytic domain and the rotating central shaft, as well as temporal control of substrate binding and product release. Despite great efforts and significant progress, the molecular details of this synchronized and fine-tuned...
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Molecular mechanism and energetics of coupling between substrate binding and product release in the F 1 -ATPase catalytic cycle
PublicationF1-ATPase is a motor protein that couples the rotation of its rotary γ subunit with ATP synthesis or hydrolysis. Single-molecule experiments indicate that nucleotide binding and release events occur almost simultaneously during the synthesis cycle, allowing the energy gain due to spontaneous binding of ADP to one catalytic β subunit to be directly harnessed for driving the release of ATP from another rather than being dissipated...
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Pale wkręcane ATLAS
PublicationOmówiono nową technologię pali ATLAS. Przedstawiono przykłady zastosowania w odniesieniu do gruntów silnie uwarstwionych. Przedyskutowano metody obliczeń.
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Pale wkręcane ATLAS
PublicationPrzedstawiono praktyczne zastosowanie nowoczesnych, przemieszczeniowych pali wkręcanych typu ATLAS. Omówiono zasady obliczeń tego rodzaju pali, również na podstawie wyników badań sondą CPT. Przykłady posadowień z terenu Polski pozwalają na ocenę mozliwości posadowień w warunkach krajowych.
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Caspase-11 promotes allergic airway inflammation
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Pressure Dependence of Acoustic Modes in AgGaSe 2
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Europejski atlas ryzyka na drogach
PublicationW maju 2008r. FRIL, PZM i PG włączyły się w realizację 3-letniego Projektu ''Europejski atlas bezpieczeństwa ruchu drogowego'' realizowanego w ramach Programu Euro-RAP przy wsparciu Komisji Europejskiej. Pod przewodnictwem EuroRAP zespoły z 10 państw opracowują krajowe Mapy ryzyka na drogach: Niemiec, Hiszpanii, Holandii, Belgii, szwecji, Wielkiej Brytanii, Włoch, Słowacji, Czech i Polski. Mapy krajowe po połączeniu utworzą Europejski...
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Doświadczenia ze stosowania pali wkręcanych ''Atlas''
PublicationNowoczesna technologia pali przemieszczeniowych typu Atlas jest coraz częściej stosowana w praktyce. Stosowanie tego rozwiązania w praktyce wymaga jednak dobrej znajomości szczegółów technologicznych i uwzględnienia parametrów, które w praktyce mogą ograniczać długość pali. Na przykładzie budowy budynku w Politechnice Gdańskiej omawiano wpływ parametrów geometrycznych pali na ich nośność oraz wpływ średnicy na krzywą osiadania.
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Experience gained at the application of Atlas piles in Poland
PublicationAnaliza współpracy pali ATLAS z uwarstwionym podłożem gruntowym na podstawie obliczeń oraz próbnych obciążeń statycznych. Przydatność pali ATLAS w terenach zurbanizowanych.
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The Motion Analysis of Attacus Atlas Rigid Wing
PublicationThe remarkable aerodynamic efficiency of flapping insect wings has fascinated researchers for many years. Butterfly wings are distinguished by a much larger lifting surface, and thus, a different style of flapping flight. The considerations described in the article are an introduction to a fully flexible analysis of the wing in motion. The study of the rigid wing gives the basic knowledge necessary in the further process. The yaw,...
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The Digital Tissue and Cell Atlas and the Virtual Microscope
PublicationWith the cooperation of the CI TASK (Center of lnformatics Tri-Citry Academic Supercomputer and network) and the Gdańsk University of Technology, the Medical University of Gdańsk undertook the creation of the Digital Tissue and Cell Atlas and the Virtual Microscope for the needs of the Bridge of Data project. In the beginning, an extensive collection of histological and cytological slides was carefully selected and prepared by...
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The wetting and interfacial properties of alumina surface treated with dipalmitoylphosphatidylcholine and lipase enzyme
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Atlas ryzyka na drogach krajowych w Polsce
PublicationEuropejski ''Program oceny ryzyka na drogach'' jest jedną z inicjatyw wspierających realizację ''wizji zero''. Program ma na celu przeprowadzenie badań ryzyka panującego na drogach krajów Unii Europejskiej, korzystając z jednolitej metodyki. Wyniki tych badań mają posłużyć do metodycznej oceny poziomu zagrożenia zdrowia i życia w ruchu drogowym. W chwili obecnej program ten realizuje ponad 30 krajów Europy, a kilka nastepnych zamierza...
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Deformable model of a butterfly in motion on the example of Attacus atlas
PublicationInsect wings can undergo significant chordwise (camber) as well as spanwise (twist) deformation during flapping flight but the effect of these deformations is not well understood. The shape and size of butterfly wings leads to particularly large wing deformations, making them an ideal test case for investigation of these effects. High-speed videogrammetry was used to capture the wing kinematics and deformations. The movements of...
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Identification and molecular modeling of a novel lipase from an Antarctic soil metagenomic library.
PublicationIn this work, we present the construction of a metagenomic library in Escherichia coli using pUC19 vector and environmental DNA directly isolated from Antarctic topsoil and screened for lipolytic enzymes. The screening on agar supplemented with olive oil and rhodamine B revealed one clone with lipolytic activity (Lip1) out of 11,000 E. coli clones. This clone harbored a plasmid, pLip1, which has an insert of 4722 bp that has been...
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Electrokinetic properties of n-tetradecane/ethanol emulsions with DPPC and enzyme lipase or phospholipase A2
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The assessment of load-settlement curve for Atlas piles correlated with CPT tests
PublicationRacjonalne stosowanie pali przemieszczeniowych typu Atlas wymaga analitycznej oceny pełnej krzywej obciążenie - osiadanie. W pracy przedstawiono możliwości wykorzystania metody funkcji transformacyjnych t-z (dla pobocznicy) oraz q-z (dla podstawy) dla oceny krzywej osiadania. Jednostkowe graniczne opory na pobocznicy, tmax, oraz pod podstawą, gf, określono na podstawie metody bezpośredniej, wykorzystując opory stożka sondy, qc....
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Molecular and structural characteristics of cod gelatin films modifiedwith EDC and TGase
PublicationCod gelatin films before and after cross-linking of gelatin with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) or transglutaminase (TGase) have been characterized by FT-IR spectroscopy and DSC analysis. For comparison, a film prepared from unmodified pig gelatin has been also analysed. The difference spectra showed that cod gelatin during the film formation involved first of all water-to-amide hydrogen bonds, and the film...
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Effect of Temperature on n-Tetradecane Emulsion in the Presence of Phospholipid DPPC and Enzyme Lipase or Phospholipase A2
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"All together to protect you" – ATLAS Network, European Special Intervention Units
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Resourcing in social enterprise – content analysis of 'good practices' profiled in 'Atlas of social economy' in Poland
PublicationThe aim of this paper is to analyze and show what processes accompany resource mobilization among social enterprises. For this, the author uses a sample of 25 ‘good practice’ examples selected and presented in ‘Atlas of social economy’ in Poland, in the most popular and well-established knowledge base web portal on social economy and social enterprise in Poland. The author makes an attempt at deconstructing the profiles of the...
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Kreowanie marki narodowej w Polsce w warunkach globalizacji rynku (na przykładzie grupy ATLAS)
PublicationTematem pracy jest budowanie marki narodowej w Polsce w warunkach globalizacji rynku. Autor omawiając pojęcie i istote marki narodowej formułuje własna jej definicję. Stara się wykazać w pracy, że marka narodowa produktu jest z punktu widzenia osiągnięcia korzyści rynkowych - pożądanym towarem i cenioną wartością, dającą możliwośc uzyskania przez tę markę przewagi konkurencyjnej na rynku.
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The atlas of inequality aversion: theory and empirical evidence on 55 countries from the Luxembourg Income Study database
PublicationResearch background: In the distributive analysis, the constant relative inequality aversion utility function is a standard tool for ethical judgements of income distributions. The sole parameter ε of this function expresses a society’s aversion to inequality. However, the profession has not committed to the range of ε. When assessing inequality and other welfare characteristics, analysts assume an arbitrary level of ε, common...
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Badanie podatności spolimeryzowanych triacylogliceroli na hydrolizę w obecności lipazy trzustkowej. Study of susceptibility polymerized triacylglycerols to hydrolytic action of pancreatic lipase.
PublicationBadano przebieg lipolizy ogrzewanego oleju rzepakowego i słonecznikowego o wysokim stopniu przemian termooksydatywnych. Wykorzystano do tego netodę HPSEC. Stopień lipolizy polimerów i dimerów TAG zależał głównie od ich ilości. W oleju o najniższym stopniu przemian (31%FP) związki te uległy lipolizie prawie w 100%, lecz przy wydłużonym czasie reakcji.
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Identification of 1H‐indene‐(1, 3, 5, 6)‐tetrol derivatives as potent pancreatic lipase inhibitors using molecular docking and molecular dynamics approach
PublicationPancreatic lipase is a potential therapeutic target to treat diet-induced obesity in humans, as obesity-related diseases continue to be a global problem. Despite intensive research on finding potential inhibitors, very few compounds have been introduced to clinical studies. In this work, new chemical scaffold 1H-indene-(1,3,5,6)-tetrol was proposed using knowledge-based approach, and 36 inhibitors were derived by modifying its...
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Ceragenin CSA-13 as free molecules and attached to magnetic nanoparticle surfaces induce caspase-dependent apoptosis in human breast cancer cells via disruption of cell oxidative balance
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Evaluation of Sicilian landraces of broccoli (B. oleracea var. italica Plenck) for quality traits
PublicationBrassica oleracea L. var. italica Plenck is one of the most popular vegetables which belong to Brassicaceae family. It is getting attention as an important source of bioactive compounds such as glucosinolates, and many other phytochemicals. In the frame of the H2020 BRESOV project, we evaluated three landraces (‘Broccolo nero', ‘Ramoso calabrese' and ‘Sparaceddu') and two commercial F1 hybrid cultivars (‘Marathon' and ‘Naxos')....
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Classification of Sea Going Vessels Properties Using SAR Satellite Images
PublicationThe aim of the project was to analyze the possibility of using machine learning and computer vision to identify (indicate the location) of all sea-going vessels located in the selected area of the open sea and to classify the main attributes of the vessel. The key elements of the project were to download data from the Sentinel-1 satellite [1], download data on the sea vessels [2], then automatically tag data and develop a detection...
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From Scores to Predictions in Multi-Label Classification: Neural Thresholding Strategies
PublicationIn this paper, we propose a novel approach for obtaining predictions from per-class scores to improve the accuracy of multi-label classification systems. In a multi-label classification task, the expected output is a set of predicted labels per each testing sample. Typically, these predictions are calculated by implicit or explicit thresholding of per-class real-valued scores: classes with scores exceeding a given threshold value...
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ColorNephroNet: Kidney tumor malignancy prediction using medical image colorization
PublicationRenal tumor malignancy classification is one of the crucial tasks in urology, being a primary factor included in the decision of whether to perform kidney removal surgery (nephrectomy) or not. Currently, tumor malignancy prediction is determined by the radiological diagnosis based on computed tomography (CT) images. However, it is estimated that up to 16% of nephrectomies could have been avoided because the tumor that had been...
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Machine learning applied to acoustic-based road traffic monitoring
PublicationThe motivation behind this study lies in adapting acoustic noise monitoring systems for road traffic monitoring for driver’s safety. Such a system should recognize a vehicle type and weather-related pavement conditions based on the audio level measurement. The study presents the effectiveness of the selected machine learning algorithms in acoustic-based road traffic monitoring. Bases of the operation of the acoustic road traffic...
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Keep It Flexible: Driving Macromolecular Rotary Motions in Atomistic Simulations with GROMACS
PublicationWe describe a versatile method to enforce the rotation of subsets of atoms, e.g., a protein subunit, in molecular dynamics (MD) simulations. In particular, we introduce a “flexible axis” technique that allows realistic flexible adaptions of both the rotary subunit as well as the local rotation axis during the simulation. A variety of useful rotation potentials were implemented for the GROMACS 4.5 MD package. Application to the...
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Analysis of Reconstituted Tripartite Complex Supports Avidity-based Recruitment of Hsp70 by Substrate Bound J-domain Protein
PublicationHsp70 are ubiquitous, versatile molecular chaperones that cyclically interact with substrate protein(s). The initial step requires synergistic interaction of a substrate and a J-domain protein (JDP) cochaperone, via its J-domain, with Hsp70 to stimulate hydrolysis of its bound ATP. This hydrolysis drives conformational changes in Hsp70 that stabilize substrate binding. However, because of the transient nature of substrate and JDP...
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Segmentation-Based BI-RADS ensemble classification of breast tumours in ultrasound images
PublicationBackground: The development of computer-aided diagnosis systems in breast cancer imaging is exponential. Since 2016, 81 papers have described the automated segmentation of breast lesions in ultrasound images using arti- ficial intelligence. However, only two papers have dealt with complex BI-RADS classifications. Purpose: This study addresses the automatic classification of breast lesions into binary classes (benign vs. ma- lignant)...
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Attention-Based Deep Learning System for Classification of Breast Lesions—Multimodal, Weakly Supervised Approach
PublicationBreast cancer is the most frequent female cancer, with a considerable disease burden and high mortality. Early diagnosis with screening mammography might be facilitated by automated systems supported by deep learning artificial intelligence. We propose a model based on a weakly supervised Clustering-constrained Attention Multiple Instance Learning (CLAM) classifier able to train under data scarcity effectively. We used a private...
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INFOGEST inter-laboratory recommendations for assaying gastric and pancreatic lipases activities prior to in vitro digestion studies
PublicationIn vitro digestion studies often use animal digestive enzyme extracts as substitutes of human gastric and pancreatic secretions. Pancreatin from porcine origin is thus commonly used to provide relevant pancreatic enzymes such as proteases, amylase and lipase. Rabbit gastric extracts (RGE) have been recently introduced to provide gastric lipase in addition to pepsin. Before preparing simulated gastric and pancreatic extracts with...
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Toksyczność ekosystemu. W: Rzeka Bug, zasoby wodne i przyrodnicze. Pod red.J. Dojlido, W. Kowalczewskiego, R. Miłaszewskiego, J. Ostrowskiego. Warsza- wa: IMGW, Wyż. Szk. Ekol. i Zarz.**2003 s. 352-361, 4 rys. 2 tab. bibliogr. 5 poz. Seria Atlasy i Monografie
PublicationOmówiono wyniki badań ekotoksykologicznych prowadzonych w ramach ''Projektupilotowego wdrożenia wytycznych monitoringu i oceny jakości wód transgrani-cznych w zlewni rzeki Bug w ramach Konwencji EKO/ONZ w ochronie i użytkowa-niu cieków transgranicznych i jezior międzynarodowych''. Badania dotyczyły o-ceny ekotoksykologicznej wód, osadów i ścieków w zlewni rzeki Bug. Badaniadowodzą, że jakość ekotoksykologiczna wód i osadów...
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Rainwater chemistry composition in Bellsund: Sources of elements and deposition discrepancies in the coastal area (SW Spitsbergen, Svalbard)
PublicationDiscrepancies in rainfall chemistry in Bellsund were found to be influenced by the orographic barrier and related to the variability in the inflow of air masses as well as to the distance of sampling sites from the sea and thus the extent of sea spray impact. This study covers measurements of rainfall (P) and air temperature (T), physicochemical parameters (pH, specific electrolytic conductivity (SEC), major ions (Cl , NO3 , SO4...
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Analysis of 2D Feature Spaces for Deep Learning-based Speech Recognition
Publicationconvolutional neural network (CNN) which is a class of deep, feed-forward artificial neural network. We decided to analyze audio signal feature maps, namely spectrograms, linear and Mel-scale cepstrograms, and chromagrams. The choice was made upon the fact that CNN performs well in 2D data-oriented processing contexts. Feature maps were employed in the Lithuanian word recognition task. The spectral analysis led to the highest word...
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Analiza dobrych praktyk ekonomii społecznej w Polsce – o zasobach i ich pozyskiwaniu w przedsiębiorczości społecznej
PublicationArtykuł, przy wykorzystaniu analizy zawartości (content analysis) przedstawionych w dobrych praktykach przedsiębiorstw społecznych i ekonomii społecznej, stanowi próbę przeanalizowania rodzajów zasobów wykorzystywanych przez przedsiębiorstwa społeczne, procesów pozyskiwania zasobów oraz trudności się w nich pojawiających. W tym celu wykorzystano profile 25 przypadków dobrych praktyk, prezentowanych i promowanych na najbardziej...
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Problemy z deszczem. Wody opadowe – ustalenia ilościowe
PublicationProblemy określenia wskaźników ilościowych przy zagospodarowaniu wód opadowych w Polsce. Wymagania formalne, problematyczność ustaleń ilościowych wg wzorów empirycznych. Tworzenie atlasu elektronicznego. Problem zdarzeń niemieszczących się typowych schematach. Zmiany wprowadzane nową ustawą prawo wodne.
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Computer-Aided Diagnosis of COVID-19 from Chest X-ray Images Using Hybrid-Features and Random Forest Classifier
PublicationIn recent years, a lot of attention has been paid to using radiology imaging to automatically find COVID-19. (1) Background: There are now a number of computer-aided diagnostic schemes that help radiologists and doctors perform diagnostic COVID-19 tests quickly, accurately, and consistently. (2) Methods: Using chest X-ray images, this study proposed a cutting-edge scheme for the automatic recognition of COVID-19 and pneumonia....
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Analyzing the Effectiveness of the Brain–Computer Interface for Task Discerning Based on Machine Learning
PublicationThe aim of the study is to compare electroencephalographic (EEG) signal feature extraction methods in the context of the effectiveness of the classification of brain activities. For classification, electroencephalographic signals were obtained using an EEG device from 17 subjects in three mental states (relaxation, excitation, and solving logical task). Blind source separation employing independent component analysis (ICA) was...
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Automatyczne urządzenia przełączające nn - oferta firmy Siemens
PublicationUkłady samoczynnego załaczania rezerwy zasilania (SZR), okrełslane w PN-EN 60947-6-1 jako automatyczne urządzenia przełaczające (ATSE ang. Automatic Transfer Switching Equipment) są przeznaczone do zapewnienia ciągłosci zasilania odbiorców energii elektrycznej. W artykule omówiono klasyfikację ATSE, budowę blokad aparatów wykonawczych oraz zalecane czasy zadzialania ATSE. Przedstawiono automatyczne urządzenia przełaczające oferowane...
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Automatyczne urządzenia przełączające niskiego napięcia
PublicationUkłady samoczynnego załaczania rezerwy zasilania (SZR), okrełslane w PN-EN 60947-6-1 jako automatyczne urządzenia przełaczające (ATSE ang. Automatic Transfer Switching Equipment) są przeznaczone do zapewnienia ciągłosci zasilania odbiorców energii elektrycznej. W artykule omówiono klasyfikację ATSE, budowę elektrycznych blokad aparatów wykonawczych oraz zalecane czasy zadzialania ATSE. Przedstawiono automatyczne urządzenia przełaczające...
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COVID-19 severity forecast based on machine learning and complete blood count data
PublicationProper triage of COVID-19 patients is a key factor in eective case management, especially with limited and insucient resources. In this paper, we propose a machine-aided diagnostic system to predict how badly a patient with COVID-19 will develop disease. The prognosis of this type is based on the parameters of commonly used complete blood count tests, which makes it possible to obtain data from a wide range of patients.We chose...
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COVID-19 severity forecast based on machine learning and complete blood count data
PublicationProper triage of COVID-19 patients is a key factor in eective case management, especially with limited and insucient resources. In this paper, we propose a machine-aided diagnostic system to predict how badly a patient with COVID-19 will develop disease. The prognosis of this type is based on the parameters of commonly used complete blood count tests, which makes it possible to obtain data from a wide range of patients.We chose...
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Changes in enzymatic activity of fish and slaughter animals meat after high pressure treatment at subzero temperatures
PublicationThe aim of this study was to determine changes in the activity of proteolytic enzymes and transglutaminase of fish and mammal meat after pressurization at subzero temperatures. The activity was measured at the optimal pHs determined for enzymes from particular types of tested meat. It was found that increasing the pressure in the range of 60–193 MPa, did not change significantly the activity of acidic proteases of cod flesh, while...