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Search results for: MOTOR PROTEINS
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Chromatin-associated microprocessor assembly is regulated by the U1 snRNP auxiliary protein PRP40
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Modeling protein structures with the coarse-grained UNRES force field in the CASP14 experiment
PublicationThe UNited RESidue (UNRES) force field was tested in the 14th Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP14), in which larger oligomeric and multimeric targets were present compared to previous editions. Three prediction modes were tested (i) ab initio (the UNRES group), (ii) contact-assisted (the UNRES- contact group), and (iii) template-assisted (the UNRES-template...
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Justification of quasi-stationary approximation in models of gene expression of a self-regulating protein
PublicationWe analyse a model of Hes1 gene transcription and protein synthesis with a negative feedback loop. The effect of multiple binding sites in the Hes1 promoter as well as the dimer formation process are taken into account. We consider three, possibly different, time scales connected with: (i) the process of binding to/dissolving from a binding site, (ii) formation and dissociation of dimers, (iii) production and degradation of Hes1...
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Fatty acyl benzamido antibacterials based on inhibition of DnaK-catalyzed protein folding
PublicationWe have reported that the hsp70 chaperone DnaK from Escherichia coli might assist protein folding by catalyzing the cis/trans isomerization of secondary amide peptide bonds in unfolded or partially folded proteins. In this study a series of fatty acylated benzamido inhibitors of the cis/trans isomerase activity of DnaK was developed and tested for antibacterial effects in E. coli MC4100 cells. Nα-[Tetradecanoyl-(4-aminomethylbenzoyl)]-l-asparagine...
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Depression as is Seen by Molecular Spectroscopy. Phospholipid- Protein Balance in Affective Disorders and Dementia
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Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials.
PublicationAIMS: The development of technology for preparing chitosan-protein scaffolds loaded with lysostaphin, which potentially could be used as dressing for wound treatment and soft tissue infections caused by Staphylococcus aureus. METHODS AND RESULTS: The unique technology of chitosan solubilization using gaseous CO(2) instead of organic or inorganic acids was used for the incorporation of lysostaphin, the enzyme that exhibits bactericidal...
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How water mediates the long-range interactions between remote protein molecules
PublicationThe high crowding of macromolecules in the cytoplasm affects the processes that occur inside a living cell. It can, for example, promote the forming of various loosely connected structures of proteins. It also means that bulk water is, essentially, not present there. The relatively thin layer of solvent that separates macromolecules may be able to participate in the long-range interactions between them and make them respond to...
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A Colorimetric Microplate Assay for DNA-Binding Activity of His-Tagged MutS Protein
PublicationA simple microplate method was designed for rapid testing DNA-binding activity of proteins. The principle of the assay involves binding of tested DNA by his-tagged protein immobilized on a nickel-coated ELISA plate, following colorimetric detection of biotinylated DNA with avidin conjugated to horseradish peroxidase. The method was used to compare DNA mismatch binding activities of MutS proteins from three bacterial species. The...
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General Mechanism of Osmolytes’ Influence on Protein Stability Irrespective of the Type of Osmolyte Cosolvent
PublicationThe stability of proteins in an aqueous solution can be modified by the presence of osmolytes. The hydration sphere of stabilizing osmolytes is strikingly similar to the enhanced hydration sphere of a protein. This similarity leads to an increase in the protein stability. Moreover, the hydration sphere of destabilizing osmolytes is significantly different. These solutes generate in their surroundings so-called “structurally different...
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Unusual structural properties of water within the hydration shell of hyperactive antifreeze protein
PublicationMany hypotheses can be encountered explaining the mechanism of action of antifreeze proteins. One widespread theory postulates that the similarity of structural properties of solvation water of antifreeze proteins to ice is crucial to the antifreeze activity of these agents. In order to investigate this problem, the structural properties of solvation water of the hyperactive antifreeze protein from Choristoneura fumiferana were...
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Assembly of Lipopolysaccharide in Escherichia coli Requires the Essential LapB Heat Shock Protein
PublicationHere, we describe two new heat shock proteins involved in the assembly of LPS in Escherichia coli, LapA and LapB (lipopolysaccharide assembly protein A and B). lapB mutants were identified based on an increased envelope stress response. Envelope stress responsive pathways control key steps in LPS biogenesis and respond to defects in the LPS assembly. Accordingly, the LPS content in ΔlapB or Δ(lapA lapB) mutants was elevated, with...
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Are stabilizing osmolytes preferentially excluded from the protein surface? FTIR and MD studies
PublicationInteractions between osmolytes and hen egg white lysozyme in aqueous solutions were studied by means of FTIR spectroscopy and molecular dynamics. A combination of difference spectra method and chemometric analysis of spectroscopic data was used to determine the number of osmolyte molecules interacting with the protein, and the preferential interaction coefficient in presented systems. Both osmolytes – L-proline and trimethylamine-N-oxide...
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Determination of viscosity changes of protein hydrolyzate solutions depending on shear rate
Open Research DataRheological characteristics of connective tissue protein solutions were made on the basis of viscosity measurement using a Brookfield viscometer using LV SC4 - 27 spindles and shear rate of 85 s-1 (Fig. 1). Solutions of protein hydrolysates with a concentration of 2% in 0.5M acetic acid were prepared.
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Proteolysis of whey protein isolates in nanoemulsion systems: impact of nanoemulsification and additional synthetic emulsifiers
PublicationNanoemulsions are currently of interest in the functional food sector because their small droplet size (100–500 nm) provides a number of potential advantages over conventional emulsions. This study concerned the behavior of nanoemulsions stabilized with whey proteins and two synthetic emulsifiers (Tween 80 and Croduret), and exposed to conditions simulating the human upper gastrointestinal tract. In particular, the effect of synthetic...
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Sensorless IPMSM drive with rotor position estimator based on analysis of phase current derivatives
PublicationThis paper describes a sensorless Interior Permanent Magnet Synchronous Motor (IPMSM) drive designed for traction applications. Wide-speed sensorless operation is provided with the use of three methods of rotor position estimation designed for: a standstill, low- and high-speed range. The paper focuses on the high-speed estimation algorithm. The estimator uses the derivatives of motor phase currentsresulting from PWM modulation...
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Molecular basis and quantitative assessment of TRF1 and TRF2 protein interactions with TIN2 and Apollo peptides
PublicationShelterin is a six-protein complex (TRF1, TRF2, POT1, RAP1, TIN2, and TPP1) that also functions in smaller subsets in regulation and protection of human telomeres. Two closely related proteins, TRF1 and TRF2, make high-affinity contact directly with double-stranded telomeric DNA and serve as a molecular platform. Protein TIN2 binds to TRF1 and TRF2 dimer-forming domains, whereas Apollo makes interaction only with TRF2. To elucidate...
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Western blot analysis of autophagy-related proteins of A549, H226 and H460 cells after exposure to C4
Open Research DataThe dataset includes measurements of the density of autophagy-related proteins in A549, H226, and H460 cell lines following exposure to compound C4.
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Clinical Implications of mTOR Expression in Papillary Thyroid Cancer—A Systematic Review
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Volume optimization of high-speed surface-mounted permanent magnet synchronous motor based on sequential quadratic programming technique and analytical solution
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Excess production of phage λ delayed early proteins under conditions supporting high Escherichia coli growth rates
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Characterization of the interactions between human high-molecular-mass kininogen and cell wall proteins of pathogenic yeasts, Candida tropicalis
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Prenatal stress leads to changes in IGF-1 binding proteins network in the hippocampus and frontal cortex of adult male rat
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Novel monovalent and multivalent recombinant proteins of Borrelia burgdorferi sensu lato with potential diagnostic value – construction and biotechnological production
PublicationThe diverse antigenic structure of Borrelia burgdorferi sensu lato (s.l.) and the low degree of protein sequence conservation between genospecies causes many limitations in serodiagnosis of Lyme disease (LD). Using expression systems based on Escherichia coli, five monovalent B. burgdorferi s.l. recombinant proteins were produced. i.e., BB0108, BB0126, BB0298, BB0323, BB0689 (each in three variants derived from Borrelia afzelii,...
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Epitope Mapping of BmpA and BBK32 Borrelia burgdorferi Sensu Stricto Antigens for the Design of Chimeric Proteins with Potential Diagnostic Value
PublicationLyme disease is a tick-borne zoonosis caused by Gramnegative bacteria belonging to the Borrelia burgdorferi sensu lato (s.l.) group. In this study, IgM- and IgG-specific linear epitopes of two B. burgdorferi sensu stricto (s.s.) antigens BmpA and BBK32 were mapped using a polypeptide array. Subsequently, two chimeric proteins BmpABBK32-M and BmpA-BBK32-G were designed to validate the construction of chimeras using the identified...
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SIMULATION AND EXPERIMENTAL RESEARCH OF CLAW POLE MACHINE WITH A HYBRID EXCITATION AND LAMINATED ROTOR CORE
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Non-Adaptive Rotor Speed Estimation of Induction Machine in an Adaptive Full-Order Observer
PublicationIn the sensorless control system of an induction machine, the rotor speed value is not measured but reconstructed by an observer structure. The rotor speed value can be reconstructed by the classical adaptive law with the integrator. The second approach, which is the main contribution of this paper, is the non-adaptive structure without an integrator. The proposed method of the rotor speed reconstruction is based on an algebraic...
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Deep slot effect in the squirrel-cage induction motors with scalar (V/F) control
PublicationQualitative characteristics of the electrical drive considerably depend on identification accuracy of math model parameters. In particular, it is depend on detection accuracy of stator active resistance r1 that is used in calculation of flux linkages, rotary speed in sensorless control systems. Paper provides analysis of influence of stator deep slot effect to stator active resistance value
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Sensorless Rotor Position Estimation of Doubly-Fed Induction Generator Based on Backstepping Technique
PublicationThis paper proposes a speed observer algorithm for the sensorless control of a doubly-fed induction generator based on classical adaptive backstepping technique. The sensorless control is shown using classic stator field oriented control which is used to active and reactive power control. Performance of the proposed algorithm of a speed observer is validated by simulation and experimental results obtained using a small-rating generator...
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Robust flux observer system with sliding mode and fuzzy logic control of Induction Motors
PublicationW pracy przedstawiono implementację nowego obserwatora strumienia wirnika silnika indukcyjnego zastosowanego w układzie sterowania polowo zorientowanego ze sterowaniem ślizgowym. Proponowany system jest niewrażliwy na zmiany rezystancji stojana silnika w wyniku zastosowania korekcyjnego sprzężenia zwrotnego. Przedstawiono porównanie układów z oraz bez zastosowania sterowania ślizgowego. Zamieszczono wyniki badań symulacyjnych...
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The dynamic performance analysis of the Micro-turbine's rotor supparted on slide and roling element bearings
PublicationThis article presents the results of investigations of dynamic characteristics of a small-dimension rotor with slide and rolling element bearings. The object of investigations was the rotor-bearing system designed for the ORC based low-power steam micro-turbine. The investigations were performed using MESWIR series programs, as well as commercial FEA software ABAQUS and MADYN 2000. The results of modal analysis of the micro-rotor...
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Axial-Flux Permanent-Magnet Dual-Rotor Generator for a Counter-Rotating Wind Turbine
PublicationCoaxial counter-rotating propellers have been widely applied in ships and helicopters for improving the propulsion efficiency and offsetting system reactive torques. Lately, the counter-rotating concept has been introduced into the wind turbine design. Distributed wind power generation systems often require a novel approach in generator design. In this paper, prototype development of axial-flux generator with a counter-rotating...
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Shaping the SPL Spectra of the Acoustic Noise Emitted by Inverter-Fed Indu- ction Motors
PublicationW artykule przedstawiono nowe podejście do zmniejszania wpływu właściwości mechanicznych maszyny na emitowany przez nią hałas akustyczny. Dokonano identyfikacji mechanicznych rezonansów maszyny wykorzystując modulację RPWM z losowym wyborem częstotliwości nośnej oraz modulację sigma delta, dla której otrzymano relatywnie płaskie widmo prądu. Skonstruowano na tej podstawie funkcję gęstości prawdopodobieństwa wyboru częstotliwości...
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Przegląd i analiza układów napędowych stosowanych w rowerach elektrycznych
PublicationStreszczenie ang. : The purpose of his article is an overview and analysis of the drive system used in electrical bikes. Classical and modern drive system are described. The stress is putted on the electrical motors used in that applications. Structure, control and power supply of most recent design are presented. The analysis applies to hub motor type Clyte 408 used as a bike drive. Authors have decided to build a filed-circuit...
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Niekorzystny wpływ przekształtnikowego zasilania PWM na silniki asynchroniczne trakcyjne
PublicationThe electric traction drives with an AC motor fed by a frequency converter are used more and more often nowadays. The most significant side effects, of a converter's output voltage forming PWM method for AC motor windings, are steep voltage changes, which can initiate several destructive phenomena. The paper presents the adverse influence of a converter common mode voltage on the motor windings' increased voltage exposure and the...
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Bezczujnikowe sterowanie trakcyjnym silnikiem IPMSM małej mocy
PublicationThis paper describes an algorithm for estimation of IPMSM angular rotor position. The algorithm uses derivatives of motor phase currents resulting from PWM modulation to obtain the rotor position. Control of the IPMSM electromagnetic torque requires a precise estimation of the rotor angular position throughout the wide speed range. This involves using a set of estimation methods switched with the dependence on the actual rotor...
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Novel DNA-binding protein from Nanoarchaeum equitans Kin4-M binds all kinds of nucleic acids
PublicationNanoarchaeum equitans is the only known representative of Archaea phylum Nanoarchaeota and stands out as one of the tiniest known living organism. What is more it has smallest genome, which is only 490.885 base pairs long. It is also one of the most compact genomes. According to predictions about 95% of the DNA encodes proteins or stable RNA. Nanoarchaeum equitans lacks genes for most vital metabolic pathways including lipid, cofactor,...
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Novel thermostable single-stranded DNA-binding protein (SSB) from Deinococcus geothermalis
PublicationW celu zbadania właściwości biochemicznych białka wiążącego się do jednoniciowego DNA (SSB) pochodzącego z Deinococcus geothermalis (DgeSSB), sklonowano gen ssb otrzymany w reakcji PCR, a następnie uzyskano wydajną biosyntezę białka DgeSSB. Gen składa się z 900 par zasad, kodujących białko złożone z 300 reszt aminokwasowych, o wyliczonej masie molekularnej równej 32.45 kDa. Sekwencja aminokwasowa wykazuje 43, 44% i 75% identyczności...
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Expression profiles and genomic organisation of group A protein phosphatase 2C genes inBrassica oleracea
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An analysis and evaluation of the WeFold collaborative for protein structure prediction and its pipelines in CASP11 and CASP12
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Adipocyte Fatty Acid‐Binding Protein as a Novel Marker of Psoriasis and Clinical Response to Acitretin
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Novel Drying Methods for Sustainable Upcycling of Brewers’ Spent Grains as a Plant Protein Source
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The effect of topical treatment and psoriasis severity on serum retinol-binding protein-4 levels
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A Data Driven Model for Predicting RNA-Protein Interactions based on Gradient Boosting Machine
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Patients with obstructive sleep apnea present with chronic upregulation of serum HIF-1α protein
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Characterization of Structural and Energetic Differences between Conformations of the SARS-CoV-2 Spike Protein
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Comparison of protein precipitation methods for various rat brain structures prior to proteomic analysis
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Similarities and Differences in the Protein Composition of Cutaneous Melanoma Cells and Their Exosomes Identified by Mass Spectrometry
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Organic solvents aggregating and shaping structural folding of protein, a case study of the protease enzyme
PublicationLow solubility of reactants or products in aqueous solutions can result in the enzymatic catalytic reactions that can occur in non-aqueous solutions. In current study we investigated aqueous solutions containing different organic solvents / deep eutectic solvents (DESs) that can influence the protease enzyme's activity, structural, and thermal stabilities. Retroviral aspartic protease enzyme is responsible for the cleavage of the...
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The plasma concentration of advanced oxidation protein products and arterial stiffness in apparently healthy adults
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Blood plasma protein and lipid profile changes in calves during the first week of life
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