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Search results for: KĄT ŚCINANIA
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A comparative study of the molecular lipophilicity indices of vitamins A and E, and of some precursors of vitamin A, estimated by HPLC and by different computation methods
PublicationIndeksy lipofilowości witamin A i E oraz wybranych prekursorów witaminy A zostały obliczone na podstawie wyników badań z wykorzystaniem wysokosprawnej chromatografii cieczowej z odwróconym układem faz (RPHPLC) i kolumn chromatograficznych typu C-8 i C-18.Uzyskano doskonałe współczynniki korelacji (>0,9999) dla wyników uzyskanych dla tych dwóch typów kolumn chromatograficznych.
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Połączenie chromatografu cieczowego ze spektrometrem mas - stare i nowe rozwiązania
PublicationChromatografia cieczowa sprzężona ze spektrometrią mas w ostatnich latach stała się główną techniką wykorzystywaną do oznaczania szerokiej gamy indywiduów chemicznych. Jednakże połączenie chromatografii cieczowej i spektrometrii mas napotkało wiele problemów, takich jak ogromna różnica ciśnień panująca w obu tych aparatach oraz zmiana stanu skupienia fazy ruchomej z ciekłej na gazową (a więc zmiana objętości). Ogromnym wyzwaniem...
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Pozostałości farmaceutyków w środowisku - problem daleki od rozwiązania
PublicationDo środowiska dostaje się coraz więcej związków pochodzenia farmaceutycznego. Zainteresowanie nimi tym bardziej wzrasta ze względu na ich niekorzystny wpływ nie tylko na środowisko, ale także na organizm ludzki. Mogą one powodować m.in. feminizację samców, wzrost odporności bakterii na antybiotyki oraz wzrost lekooporności ludzi. Dodatkowo farmaceutyki mogą przekształcać się w inne związki, których potencjał wywoływania efektów...
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Enhanced Sympathetic and Ventilatory Responses to Central Chemoreflex Activation in Heart Failure
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Increases in leptin levels, sympathetic drive, and weight gain in obstructive sleep apnea
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Determination of benzodiazepines and Z-hypnotic drugs in whole blood samples by GC–MS/MS: Method development, validation and application
PublicationBenzodiazepines (BZDs) and Z-drugs are widely used as anxiolytics, sedative hypnotics, anticonvulsants and muscle relaxants. “Designer benzodiazepines” (DBZDs) are a new psychoactive substance class consisting of benzodiazepine derivatives that are not allowed for medical use and are known for being used recreationally. From a toxicologist standpoint, the huge number of such substances implicate a necessity for developing fast...
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The Influence of the Application of EP Additive in the Minimum Quantity Cooling Lubrication Method on the Tool Wear and Surface Roughness in the Process of Turning 316L Steel
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Lipidomika mleka kobiecego - wiele do odkrycia.
PublicationMleko kobiece jest wieloskładnikową wydzieliną, której skład zależy od wielu czynników, dlatego różni się międzyosobniczo, jest niepowtarzalne i oryginalne. W każdym mleku można wyróżnić zarówno składniki odżywcze niezbędne do prawidłowego wzrostu niemowlęcia, jak i bioaktywne substancje chroniące niemowlę przed zakażeniami, stanami zapalnymi oraz przyczyniającymi się do dojrzewania układu odpornościowego Ze względu na zawartość...
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Essential oils as potential anti-staphylococcal agents
PublicationAntibiotic therapy of staphylococcal mastitis is characterized by significantly lower cure rates compared to infections caused by other microorganisms. Thus, it is necessary to search for new, alternative, non-antibiotic agents that are effective in the eradication of these bacteria. The aim of our research was to investigate the antimicrobial, especially anti-staphylococcal potential of a large collection (n=36) of essential oils...
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Chemistry of human breast milk – a comprehensive review of the composition and role of milk metabolites in child development
PublicationEarly nutrition has an enormous influence on a child's physiological function, immune system maturation and cognitive development. Human breast milk (HBM) is recognized as the gold standard for human infant nutrition. Accordingly to WHO report breastfeeding is consider as an unequalled way of providing ideal food to the infant, which is required for his healthy growth and development. HBM contains various macronutrients (carbohydrates,...
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Antibiotic resistance in Staphylococcus aureus strains isolated from cows with mastitis in the eastern Poland and analysis of susceptibility of resistant strains to alternative non-antibiotic agents: lysostaphin, nisin and polymyxin B.
PublicationThe aim of the studies was analysis of resistance of Staphylococcus aureus isolates from bovine mastitis in eastern part of Poland to a set of 20 antibiotics and three alternative agents: lysostaphin, nisin and polymyxin B. Eighty-six out of 123 examined isolates were susceptible to all of 20 tested antibiotics (70 %). The highest percentage of resistance was observed in the case of β-lactam antibiotics: amoxicillin (n=22, 17.9...
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New sample preparation strategies for comprehensive lipidomics of human breast milk
PublicationGLOBAL PROFILING ANALYSIS OF HUMAN BREAST MILK (HBM) LIPIDS IS HINDERED BY COMPLEX COMPOSITION OF HBM. THIS PROBLEM IS HUGE FROM ANALYTICAL POINT OF VIEW, SINCE DUE TO THAT DETECTION OF ALL HBM LIPIDS DURING ONE ANALYTICAL RUN IS LIMITED. THUS, SAMPLE PREPARATION STEP CONSTITUTES A CRUCIAL STEP IN UNTARGETED LIPIDOMIC ANALYSIS OF HBM, ESPECIALLY WHEN SEMI-QUANTITATIVE ANALYSIS IS ASSUMED. HEREIN, WE PRESENT A COMPARISON OF PUBLISHED...
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Flavin monooxygenases, FMO1 and FMO3, not cytochrome p450 isoenzymes, contribute to metabolism of anti-tumour triazoloacridinone, C-1305, in liver microsomes and HepG2 cells.
PublicationCelem pracy było określenie roli wybranych enzymów frakcji mikrosomalnej komórek wątroby w metabolizmie pochodnej triazoloakrydonu, związku C-1305. Wykazano, że badana pochodna ulega transformacji wobec frakcji enzymów izolowanych z hepatocytów szczurzych i ludzkich oraz jest metabolizowana przez komórki linii HepG2. Badania wykazały ponadto, że enzymami odpowiedzialnymi za obserwowane przemiany były monooksygenazy flawinowe, FMO1...
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Cardinal regenerative features of the MRL mouse
PublicationIn this review, we discuss recent studies relating to major features of adult MRL mouse biology that contribute to the regenerative responses seen. These include an increased inflammatory cell profile, a unique glycolytic metabolic state typically found during embryogenesis, and a cell cycle phenotype of DNA damage and G2/M arrest. These traits have been found in other mammalian and non-mammalian regenerative systems. How these...
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The Influence of Storage on Human Milk Lipidome Stability for Lipidomic Studies
PublicationHuman milk (HM) lipidome stability during storage is crucial in lipidomic studies to avoid misinterpretations. Facing the lack of comprehensive work on the HM lipidome stability, we performed a study on a potential alteration in the lipid profiles of HM samples stored under different conditions. An untargeted LC-Q-TOF-MS-based approach was applied to study the influence of storage conditions as well as the interaction of the storage...
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Novel Interpolation Method of Multi-DFT-Bins for Frequency Estimation of Signal with Parameter Step Change
PublicationThe IpDFT(Interpolation Discrete Fourier Trans-form) method is one of the most commonly used non-parametric methods. However, when a parameter (frequency, amplitude or phase) step changes in the DFT period, the DFT coefficients will be distorted seriously, resulting in the large estimation error of the IpDFT method. Hence, it is a key challenge to find an IpDFT method, which not only can eliminate the effect of the step-changed...
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Antibiotic Resistance of Uropathogens Isolated from Patients Hospitalized in District Hospital in Central Poland in 2020
PublicationThe aim of this study was to determine antibiotic resistance patterns and the prevalence of uropathogenes causing urinary tract infections (UTIs) in patients hospitalized in January–June 2020 in central Poland. Antimicrobial susceptibility testing was performed using the disk-diffusion method. Escherichia coli (52.2%), Klebsiella pneumoniae (13.7%), Enterococcus faecalis (9.3%), E. faecium (6.2%), and Proteus mirabilis (4,3%) were...
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Model-based identification of the dominant N2O emission pathway in a full-scale activated sludge system
PublicationActivated sludge models (ASMs), extended with an N2O emission module, are powerful tools to describe the operation of full-scale wastewater treatment plants (WWTPs). Specifically, such models can investigate the most contributive N2O production pathways and guide towards N2O and carbon footprint (CF) mitigation measures. A common practice is to develop and validate models using data from a single WWTP. In this study, a successfully...
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Characterization of GM3 Gangliosides in Human Milk throughout Lactation: Insights from the Analysis with the Use of Reversed-Phase Liquid Chromatography Coupled to Quadrupole Time-Of-Flight Mass Spectrometry
PublicationGangliosides are complex lipids found in human milk that play important structural and biological functions. In this study, we utilized reversed-phase liquid chromatography coupled to quadrupole time-of-flight mass spectrometry to evaluate the molecular distribution of GM3 in human milk samples collected at distinct lactation stages, ranging from colostrum to advanced lactation samples. Throughout lactation, GM3 d40:1 emerged as...
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State of the art in sample preparation for human breast milk metabolomics—merits and limitations
PublicationHuman breast milk (HBM) contains metabolites that are diverse in chemical structure and concentration. These metabolites range from high-abundance to low-abundance compounds and from polar to nonpolar. This complex nature of HBM makes HBM samples difficult to analyze, especially in global metabolomic studies for the determination and characterization of complete sets of metabolites. In this review, the state of the art in sample...
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Comparative Lipidomic Study of Human Milk from Different Lactation Stages and Milk Formulas
PublicationIn this report, we present a detailed comparison of the lipid composition of human milk (HM) and formula milk (FM) targeting different lactation stages and infant age range. We studied HM samples collected from 26 Polish mothers from colostrum to 19 months of lactation, along with FM from seven brands available on the Polish market (infant formula, follow-on formula and growing-up formula). Lipid extracts were analysed using liquid...
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Rapid Characterization of the Human Breast Milk Lipidome Using a Solid-Phase Microextraction and Liquid Chromatography-Mass Spectrometry-Based Approach.
PublicationHuman breast milk (HBM) is a biofluid consisting of various biomolecules such as proteins, lipids, carbohydrates, minerals and bioactive substances. Due to its unique and complex composition, HBM provides not only nutritional components required for the growth of the infant, but also additional protection against infections. Global insight into the composition of HBM is crucial to understanding the health benefits infants receive...
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Analysis of chiral pharmaceutical residues in influent and effluent samples at racemic and enantiomeric level using liquid chromatography-tandem mass spectrometry
PublicationIn this work, two different chromatographic methods for seasonal monitoring of pharmaceutical residue in wastewater samples were developed. In the case of enantiomeric separation of selected compounds, LC-MS technique combining with vancomycin based chiral stationary phase was used. The performance of chiral analysis enabled to monitor the pharmaceutical contamination at the enantiomeric level. The d-SPE procedure was developed...
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Untargeted Lipidomics Analysis of the Cyanobacterium Synechocystis sp. PCC 6803: Lipid Composition Variation in Response to Alternative Cultivation Setups and to Gene Deletion
PublicationCyanobacteria play an important role in several ecological environments, and they are widely accepted to be the ancestors of chloroplasts in modern plants and green algae. Cyanobacteria have become attractive models for metabolic engineering, with the goal of exploring them as microbial cell factories. However, the study of cyanobacterial lipids’ composition and variation, and the assessment of the lipids’ functional and structural...
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LC-MS/MS dtermination of isoprostanes in plasma samples collected from mice exposed to doxorubicin or tert-butyl hydroperoxide
PublicationIsoprostanes are stable products of arachidonic acid peroxidation and are regarded as the most reliable markers of oxidative stress in vivo. Here we describe the LC-MS/MS procedure enabling simultaneous determination of four regioisomers (8-iso prostaglandin F2α, 8-iso-15(R)-prostaglandin F2α, 11β-prostaglandin F2α, 15(R)-prostaglandin F2α) in plasma samples collected from mice. The four plasma isoprostanes are determined by LC–ESI-MS/MS...
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Analiza Matematyczna 1 MAT 1001/1 (2021/22)
e-Learning CoursesAnaliza Matematyczna 1 semestr 1 kod MAT 1001/1 rok 21/22
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WCh - Ch - Mat. ćw. s2: 23/24 (D.Garbowska)
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Phototransformation of three selected pharmaceuticals, naproxen, 17α-Ethinylestradiol and tetracycline in water: Identification of photoproducts and transformation pathways
PublicationTransformation products (TPs) may be formed during various processes, however, it was proved that one of the main mechanisms responsible for degradation of pharmaceuticals in natural waters is photolysis. Three compounds were selected, tetracycline (TC), 17-α-ethinylestradiol (EE2) and naproxen (NAP), for degradation experiments which were performed using a xenon lamp emitting light with a spectrum closely approximating that of...
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SMALL INTESTINE, Small intestine, NOS - Male, 0 - Tissue image [7060729599328101]
Open Research DataThis is the histopathological image of SMALL INTESTINE tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Other specified diseases of oesophagus - Female, 14 - Tissue image [328073002560781]
Open Research DataThis is the histopathological image of ESOPHAGUS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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COLON, Right colon - Female, 79 - Tissue image [820072959221591]
Open Research DataThis is the histopathological image of COLON tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Other preterm infants - Male, 0 - Tissue image [3300730069417411]
Open Research DataThis is the histopathological image of SMALL INTESTINE tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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OTHER AND ILL-DEFINED SITES, Thoracic wall, NOS - Male, 56 - Tissue image [10060730015617081]
Open Research DataThis is the histopathological image of OTHER AND ILL-DEFINED SITES tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Other preterm infants - Male, 0 - Tissue image [3300730069415471]
Open Research DataThis is the histopathological image of COLON tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Other specified diseases of oesophagus - Female, 14 - Tissue image [3280730025608491]
Open Research DataThis is the histopathological image of ESOPHAGUS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Chemia środowiska _Chemia_sem.6 - 2023
e-Learning CoursesPoznanie i zrozumienie procesów zachodzących w trzech systemach środowiskowych (atmosferze, hydrosferze i geosferze). Ocena wpływu działalności człowieka na środowisko oraz zdefiniowanie zagrożeń związanych z zakłóceniami równowagi środowiskowej. Wyjaśnienie zmian zachodzących w środowisku i zjawisk powstających na skutek rozwoju cywilizacji – omówienie zanieczyszczeń poszczególnych ekosystemów oraz analiza możliwości ochrony ekosystemów...
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Podstawy ochrony środowiska - 2023/2024
e-Learning CoursesI rok, semestr 1 Celem przedmiotu jest zdobycie podstawowej wiedzy o środowisku i procesach w nim zachodzących. Dotyczy ogólnej edukacji proekologicznej.
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Chemia środowiska _Chemia_sem. 6 - 2024
e-Learning CoursesPoznanie i zrozumienie procesów zachodzących w trzech systemach środowiskowych (atmosferze, hydrosferze i geosferze). Ocena wpływu działalności człowieka na środowisko oraz zdefiniowanie zagrożeń związanych z zakłóceniami równowagi środowiskowej. Wyjaśnienie zmian zachodzących w środowisku i zjawisk powstających na skutek rozwoju cywilizacji – omówienie zanieczyszczeń poszczególnych ekosystemów oraz analiza możliwości ochrony ekosystemów...
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NOWOCZESNE TECHNIKI ANALITYCZNE Wykład
e-Learning CoursesStudenci zostaną zapoznani z nowoczesnymi technikami analitycznymi wykorzystywanymi do celów analizy i monitoringu zanieczyszczeń środowiska.
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MEMS based voice message system for elevators
PublicationW artykule przedstawiono implementację systemu głosowych komunikatów w windach. Prezentowany system posiada unikalną cechę polegającą na tym, że do działania nie potrzebuje połączenia z systemem sterującym windy. Zasilany z baterii lub akumulatorów może być zamontowany w ścianie windy, wymaga tylko prostej kalibracji. System oparty jest na akcelerometrach MEMS dokonujących pomiaru przeciążeń w kabinie windy. W artykule przedstawiono...
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Wyłączniki różnicowoprądowe w ochronie przeciwporażeniowej przy odkształconym prądzie różnicowym
PublicationW pracy przedstawiono rozwój konstrukcji wyłączników różnicowoprądowych, problematykę ich niezawodności oraz przeprowadzono szczegółową analizę i badania wpływu na wyzwalanie wyłączników różnicowoprądowych: częstotliwości sinusoidalnego prądu różnicowego, wyższych harmonicznych odkształconego prądu różnicowego przemiennego i kąta opóźnienia prądu różnicowego. Rozważania te uzupełniono analizą i badaniami wpływu na wyzwalanie wyłączników...
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The contactless method of chip-to-chip high-speed data transmission monitoring
PublicationThis paper presents a technique of decoupling differential signals transmitted in a pair of microstrip lines on a printed circuit board (PCB), using dedicated coupler for high speed data transmission monitoring in chip-to-chip interconnections. The coupler used for signal probing is overlayed on the pair of microstrip lines under test, and provides a signal to the next blocks of the measurement system without disturbing transmission...
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Degradowalne poli(estrouretany) z cykloalifatycznego diizocyjanianu i kopoliestrodioli z L-laktydu i poli(E-kaprolaktono)diolu
PublicationOpisano syntezę liniowych poli(estrouretanów) (PESU) z 4,4´-diizocyjanianudicykloheksylenometanu, 1,4-butanodiolu i kopoliestrodioli z L-laktydu (LA)i poli(E-kaprolaktono)diolu (PCL, Mn 560g/mol). Kopoliestrodiole o Mn 760,1250 i 3390 g/mol otrzymano przy trzech stosunkach masowych PLA i PCL w obecności oktanianu Sn(II). PESU otrzymane przy różnych stosunkach molowych komponentów scharakteryzowano przez pomiary twardości, wytrzymałości...
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Wytrzymałość materiałów, L, MiBM, Ist, sem. 03, zimowy 23/24, (PG_00055379)
e-Learning CoursesSpis treści prezentowanych na kursie: 1) Badanie twardości metali. 1) Statyczna próba rozciągania metali. 2) Statyczna próba ściskania metali. 3) Badanie udarności metali. Dynamiczna próba rozciągania metali. 4) Wyznaczenie modułu sprężystości podłużnej, umownej granicy sprężystości i umownej granicy plastyczności. 5) Statyczna próba skręcania metali. 7) Badanie odkształcenia belki za pomocą tensometrii oporowej.
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Wytrzymałość materiałów, L, ZiIP, Ist, sem. 03, zimowy 23/24, (PG_00055053)
e-Learning CoursesSpis treści prezentowanych na kursie: 1) Badanie twardości metali. 1) Statyczna próba rozciągania metali. 2) Statyczna próba ściskania metali. 3) Badanie udarności metali. Dynamiczna próba rozciągania metali. 4) Wyznaczenie modułu sprężystości podłużnej, umownej granicy sprężystości i umownej granicy plastyczności. 5) Statyczna próba skręcania metali. 7) Badanie odkształcenia belki za pomocą tensometrii oporowej.
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Modelling of Capillary Pressure-driven Water Flow in Unsaturated Concrete Using Coupled DEM/CFD Approach.
PublicationSformułowano nowatorskie, połączone podejście do modelowania dwufazowego przepływu wody napędzanego kapilarami w nienasyconym betonie. Dzięki połączeniu metody elementów dyskretnych (DEM) z obliczeniową dynamiką płynów (CFD) w warunkach izotermicznych proces został zbadany numerycznie w mezoskali w warunkach dwuwymiarowych. Niewielkie próbki betonu o uproszczonej mezostrukturze cząstek poddano w pełni sprzężonym hydromechanicznym...
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Rhabdomyosarcoma, NOS - Male, 3 - Tissue image [5280730021703551]
Open Research DataThis is the histopathological image of BLADDER tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Stomach, unspecified - Male, 75 - Tissue image [9180729562254311]
Open Research DataThis is the histopathological image of STOMACH tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Stomach, unspecified - Male, 75 - Tissue image [9180729562252011]
Open Research DataThis is the histopathological image of STOMACH tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Preterm labour with preterm delivery - Male, 0 - Tissue image [3280730025625531]
Open Research DataThis is the histopathological image of COLON tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: VS200 Olympus slide scanner (20x magnification) and saved to DICOM format.