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PCR-Based Genotyping of Mycobacterium tuberculosis with New GC-Rich Repeated Sequences and IS6110 Inverted Repeats Used as Primers.
PublicationW pracy przedstawiono nową metodę genotypowania Mycobacterium tuberculosis. Zastosowanie oligonukleotydu Mtb1 5'-CCG-GCG-GGG-CCG-GCG-G lub Mtb2 5'-CGG-CGG-CAA-CGG-CGG-C wraz z oligonukleotydami specyficznymi do terminalnych sekwencji elementu insercyjnego IS6110 pozwala na różnicowanie szczepów Mycobacterium tuberculosis w metodzie PCR. Opracowana metoda może być wykorzystana w badaniach epidemiologicznych gruźlicy.
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Malignant neoplasm of breast - Female, 47 - Tissue image [2220630014957431]
Open Research DataThis is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Malignant neoplasm of breast - Female, 47 - Tissue image [222063001495381]
Open Research DataThis is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Chronic viral hepatitis B without delta-agent - Male, 33 - Tissue image [4120730022898851]
Open Research DataThis is the histopathological image of LIVER AND INTRAHEPATIC BILE DUCTS tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Malignant neoplasm of breast - Female, 47 - Tissue image [2220630014951821]
Open Research DataThis is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Malignant neoplasm of breast - Female, 47 - Tissue image [2220630014953971]
Open Research DataThis is the histopathological image of BREAST tissue sample obtained in Medical University Gdańsk and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 250 3DHistech slide scanner (20x magnification) and saved to DICOM format.
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Low frequency of itraconazole resistance found among avian Aspergillus fumigatus isolates
PublicationAspergillus fumigatus is the most frequent etiological factor of invasive aspergillosis in human as well as animals, including birds. Aspergillosis caused by drug-resistant strains poses a significant therapeutic issue, especially if resistance pattern includes insensitivity to triazoles, i.e. drugs recommended for the primary therapy purposes. Majority of resistant isolates hold the TR34/L98H...
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CERVIX UTERI, Exocervix - Female, 45 - Tissue image [4100730020138901]
Open Research DataThis is the histopathological image of CERVIX UTERI tissue sample obtained in University of Warmia and Mazury in Olsztyn and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.
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CERVIX UTERI, Exocervix - Female, 45 - Tissue image [7040247104945251]
Open Research DataThis is the histopathological image of CERVIX UTERI tissue sample obtained in University of Warmia and Mazury in Olsztyn and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.
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CERVIX UTERI, Exocervix - Female, 45 - Tissue image [4100730020139401]
Open Research DataThis is the histopathological image of CERVIX UTERI tissue sample obtained in University of Warmia and Mazury in Olsztyn and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.
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CERVIX UTERI, Exocervix - Female, 45 - Tissue image [4100730020132581]
Open Research DataThis is the histopathological image of CERVIX UTERI tissue sample obtained in University of Warmia and Mazury in Olsztyn and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.
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Endometrioid adenocarcinoma, NOS - Female, 70 - Tissue image [4100730020138601]
Open Research DataThis is the histopathological image of CORPUS UTERI tissue sample obtained in University of Warmia and Mazury in Olsztyn and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.
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CERVIX UTERI, Exocervix - Female, 45 - Tissue image [4100730020136201]
Open Research DataThis is the histopathological image of CERVIX UTERI tissue sample obtained in University of Warmia and Mazury in Olsztyn and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.
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CERVIX UTERI, Exocervix - Female, 45 - Tissue image [410073002013641]
Open Research DataThis is the histopathological image of CERVIX UTERI tissue sample obtained in University of Warmia and Mazury in Olsztyn and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.
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Endometrioid adenocarcinoma, NOS - Female, 70 - Tissue image [4100730020137691]
Open Research DataThis is the histopathological image of CORPUS UTERI tissue sample obtained in University of Warmia and Mazury in Olsztyn and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.
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CERVIX UTERI, Exocervix - Female, 45 - Tissue image [4100730020138101]
Open Research DataThis is the histopathological image of CERVIX UTERI tissue sample obtained in University of Warmia and Mazury in Olsztyn and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.
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Endometrioid adenocarcinoma, NOS - Female, 70 - Tissue image [4100730020132661]
Open Research DataThis is the histopathological image of CORPUS UTERI tissue sample obtained in University of Warmia and Mazury in Olsztyn and deposited in ZMDL-GUMED. The sample image was taken using: Pannoramic 1000 slide scanner (20x magnification) and saved to DICOM format.
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Dominika Wróblewska dr inż. arch.
PeopleDr. Eng. arch. Dominika Wróblewska, university professor, obtained the title of doctor of technical sciences in 2000. In 2002, she started working at the Faculty of Hydro and Environmental Engineering at the Gdańsk University of Technology (currently the Faculty of Civil and Environmental Engineering) as an assistant professor. Since 2019, he has been working as a university professor. The areas of interest are changes, introducing...
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Micellar solubilization of mineral and synthetic oils in nonionic surfactant solutions
PublicationZbadano solubilizację micelarną olejów mineralnych i syntetycznych (PAO6) w wodnych roztworach surfaktantów niejonowych (oksyetylenowanych alkoholi tłuszczowych). Dodatek kosurfaktanta (alkoholu amylowego) powodował znaczny wzrost efektywności solubilizacji badanych olejów. Najwyższą efektywność solubilizacji uzyskano dla roztworów Rokanolu NL6 (50 g/dm3) z dodatkiem alkoholu amylowego (2-2.5%). Ze wzrostem liczby grup oksyetylenowych...
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Wpływ dezintegracji termicznej oraz ultradźwiękowej na podatność osadów ściekowych na odwadnianie
PublicationBadano wpływ dezintegracji termicznej oraz ultradźwiękowej osadów ściekowych na ich podatność na odwadnianie. Dzintegrację prowadzono dla osadów ściekowych o zawartości suchej masy 4,8 i 12 g/l pzry trzech czasach sonifikacji i dla zakresu temperatur od 60 do 100 stopni Celsjusza. Zmiany podatności na odwadnianie badano testem czasu ssania kapilarnego (CSK) oraz testem wirówkowym oznaczając stopień rozdziału.
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Immobilization of thermostable beta glucosidase from Sulfolobus shibatae by cross-linking with transglutaminase
PublicationOpracowano metodę immobilizacji termostabilnej beta-glukozydazy przez katalizowane transglutaminazą sieciowanie na nośniku krzemionkowym. Aktywność uzyskanego preparatu wynosi 3883 U/g nośnika. Największą wydajność unieruchamiania uzyskano w temperaturze 50C, przy pH 5,0. Immobilizacja prawie nie zmieniała optymalnego pH działania enzymu, który uzyskiwał największą aktywność w temperaturze 98C. Badania w reaktorze kolumnowym wykazały...
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Chloroacridine derivatives as potential anticancer agents which may act as tricarboxylic acid cycle enzyme inhibitors
PublicationThe chloroacridines affect biological forms of melanoma in different ways. Amelanotic (Ab) melanoma (with inhibited melanogenesis and higher malignancy) was particularly sensitive to the action of the chloroacridines. The Ab melanoma cells died through apoptosis and through death without caspase activation. Diminished activity of TAC enzymes was noticed among Ab melanoma cells together with ATP/NAD depletion, especially in the...
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Effects of CO2 and utilization of spent trickling liquid towards plant growth
Open Research DataDataset presents the results of biomass yield for Phaseolus vulgaris pot cultures watered with diluted trickling liquid from biotrickling filtration experiments with or without additional CO2 in air by means of introducting post-biofilter air to the plant culture.
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INVESTIGATION OF THE EFFECT OF THE ROCKET'S SUBORBITAL FLIGHT ON BIOFILM, ENZYMES AND BIOSYNTHESIS ON AUTONOMOUS, MODULAR AND SCALABLE PLATFORM FOR CONDUCTING EXPERIMENTS OF AN ASTROBIOTECHNOLOGICAL NATURE
PublicationWith new incentives for human space exploration, biotechnological experiments in orbit became imperative.Answering this need, we build an autonomous, modular, and scalable platform that enables those experiments onrockets. We called it AMBER. The aim of the payload of the R6 suborbital rocket is to perform an experiment tostudy the influence of rocket flights on biofilm, molecular...
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Combining Computational Fluid Dynamics with a Biokinetic Model for Predicting Ammonia and Phosphate Behavior in Aeration Tanks
PublicationThe aim of this study was to use computational fluid dynamics for predicting the behavior of reactive pollutants (ammonia and phosphate) in the aerobic zone of the bioreactor located at the Wschod wastewater treatment plant in Gdansk, Poland. The one-dimensional advection-dispersion equation was combined with simple biokinetic models incorporating the Monod-type expressions as source terms for the two pollutants. The problem was...
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Evaluation of citric acid corrosion inhibition efficiency and passivation kinetics for aluminium alloys in alkaline media by means of dynamic impedance monitoring
PublicationA novel approach is proposed for constructing the adsorption isotherm in corrosion studies, based on a well-known interaction between citric acid and aluminium in alkaline electrolytes. Our approach utilizes the instantaneous impedance measurements via Dynamic Electrochemical Impedance Spectroscopy in galvanostatic mode (g-DEIS). Unlike other common tools, g-DEIS delivers exact information about the concentration required for full...
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Analysis of bisphenols and their derivatives in infant and toddler ready-to-feed milk and powdered milk by LC MS/MS
PublicationReady-to-feed milk and powdered milk packed in different types of packaging lined with resin are chosen as the primary or complementary diet for infants and toddlers. An HPLC–MS/MS method was established and validated for the determination of nineteen bisphenols and their derivatives in such samples. The recoveries were from 31% to 120%, while the values of limit of detection and quantification were in the range of 0.24–0.40 and...
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Polychlorinated biphenyls (PCBs) and polychlorodibenzo-p-dioxins (PCDDs) determination in disposable baby diapers with the application of natural deep eutectic solvent
PublicationIn this work a new method involving solvent extraction of porous membrane-packed solid samples (SE-PMSS) coupled to gas chromatography-mass spectrometry (GC–MS) has been developed for the determination of six polychlorinated biphenyls (PCBs) and five polychlorodibenzo-p-dioxines (PCDDs) in disposable baby diapers. In that aim, a terpenoid-based natural deep eutectic solvent (NADES) composed of carvone and camphor in a 1:1 M ratio...
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Development of a liquid chromatography–tandem mass spectrometryprocedure for determination of endocrine disrupting compounds infish from Mediterranean rivers
PublicationA new, sensitive and rapid method based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe)approach followed by ultra high performance liquid chromatography coupled with tandem mass spec-trometry (UHPLC–MS/MS) was developed for the determination of nineteen endocrine disruptors (EDCs)and related compounds belonging to different classes in various fish species. Matrix effect on the analyti-cal performance was evaluated,...
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The Influence of the Electrodeposition Parameters on the Properties of Mn-Co-Based Nanofilms as Anode Materials for Alkaline Electrolysers
PublicationIn this work, the influence of the synthesis conditions on the structure, morphology, and electrocatalytic performance for the oxygen evolution reaction (OER) of Mn-Co-based films is studied. For this purpose, Mn-Co nanofilm is electrochemically synthesised in a one-step process on nickel foam in the presence of metal nitrates without any additives. The possible mechanism of the synthesis is proposed. The morphology and structure...
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Exploring multi-step glucose oxidation kinetics at GOx-functionalized nanotextured gold surfaces with differential impedimetric technique
PublicationFor a few past years, we can observe the enormous growth of investigations related to ultrasensitive electrochemical sensors capable of reliable determination of important body parameters and analytes. Utilized procedures rely on standard electrochemical methods, demanding electrode polarization, and information about the initial characteristics of the working electrode. More and more complex electrode materials are characterized...
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Bioactivities of Phenolic Compounds from Kiwifruit and Persimmon
PublicationFruit used in the common human diet in general, and kiwifruit and persimmon particularly, displays health properties in the prevention of heart disease. This study describes a combination of bioactivity, multivariate data analyses and fluorescence measurements for the differentiating of kiwifruit and persimmon, their quenching and antioxidant properties. The metabolic differences are shown, as well in the results of bioactivities...
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A miniaturized solid-contact potentiometric multisensor platform for determination of ionic profiles in human saliva
PublicationThis paper describes a miniaturized multisensor platform (MP-ISES) consisting of electrodes: a reference one (RE) and ion-selective electrodes (ISEs) for monitoring Na+, K+, Ca2+, Mg2+, Cl−, and SCN− ions and pH in human saliva. Gold electrode surface was modified by deposition of two layers: electrosynthesized PEDOT:PSS forming an intermediate layer, and ion-selective membrane. The developed ISEs were characterized by a wide linear...
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Exploring the sequence-structure relationship as a starting point for the design of DNA G-quadruplexes with a given topology -- an integrative approach combining molecular simulations with experimental methods
ProjectsProject realized in Department of Physical Chemistry according to UMO-2019/35/B/ST4/03559 agreement from 2020-06-19
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Wpływ równości nawierzchni i dynamicznego oddziaływania pojazdów ciężkich na trwałość zmęczeniową nawierzchni podatnych
PublicationPodczas ruchu pojazdu obserwuje się odchylenia nacisków osi pojazdów od nacisku statycznego, co jest spowodowane nierównościami nawierzchni. Dynamiczne obciążenia pojazdów w większym stopniu wytężają konstrukcję nawierzchni drogowej. Rozkład obciążeń dynamicznych ma kształt rozkładu normalnego i jest opisywany poprzez obciążenie statyczne oraz wskaźniki dynamiczne DI lub DLC. Wraz z pogorszeniem równości i zwiększeniem prędkości...
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Regulation of replication of λ phage and λ plasmid DNAs at low temperature
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POSTHARVEST CALCIUM CHLORIDE DIPS FOR INCREASING PEACH FIRMNESS
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SIMULATING CALCIUM DIP EFFECT ON POST HARVEST QUALITY OF PEACHES
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Development of low noise CCD readout front-end
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Development of low noise CCD readout front-end
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Blood pressure disturbances and endothelial dysfunction markers in children and adolescents with type 1 diabetes
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Climate Change Impacts on Environmental and Human Exposure to Mercury in the Arctic
PublicationThis paper reviews information from the literature and the EU ArcRisk project to assess whether climate change results in an increase or decrease in exposure to mercury (Hg) in the Arctic, and if this in turn will impact the risks related to its harmful effects. It presents the state-of-the art of knowledge on atmospheric mercury emissions from anthropogenic sources worldwide, the long-range transport to the Arctic, and it discusses...
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Critical Remarks on Landauer’s principle of erasure– dissipation: Including notes on Maxwell demons and Szilard engines
PublicationWe briefly address Landauer’s Principle and some related issues in thermal demons. We show that an error-free Turing computer works in the zero-entropy limit, which proves Landauer’s derivation incorrect. To have a physical logic gate, memory or information-engine, a few essential components necessary for the operation of these devices are often neglected, such as various aspects of control, damping and the fluctuation–dissipation...
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Application of multivariate mathematical-statistical methods to compare reversed-phase thin-layer and liquid chromatographic behaviour of tetrazolium salts in Quantitative Structure-Retention Relationships (QSRR) studies
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Cavitation erosion of heterogeneous thin coatings produced by means of laser cladding
PublicationPrzedstawiono badania odporności kawitacyjnej cienkich heterogenicznych warstw nakładanych metodami laserowymi na stal 45. Wykazano dobrą przyczepność i wysoką odporność kawitacyjną wykonanych powłok.
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Nitrogen transformation during fermentation in agricultural biogas plants
PublicationThe aim of the present study was to determine changes in the organic nitrogen fraction in the liquid fraction of the digestate during laboratory tests of mono- and co-digestion. Three agricultural wastes were tested: distillery residue, cattle slurry and corn silage. In the mono-fermentation tests, the initial total nitrogen concentration depended on the feedstock used. As a result of the hydrolysis and ammonification of the organic...
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CARDIOVASCULAR RISK FACTORS AMONG POLISH EMPLOYEES OF UNIFORMED SERVICES
PublicationObjectives: Employees of uniformed services (EoUS) were screened for cardiovascular risk factors. Material and Methods: A total of 1138 EoUS (age M±SD 49.9±6.0 years) and 263 controls (age M±SD 54.4±9.7 years) under the care of the cardiology clinic in Gdańsk, Poland,were included in the study. Medical history and blood samples were collected, and a physical examination was performed. Ten-year cardiovascular risk of death was...
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Potentiometric Solid-Contact Ion-Selective Electrode for Determination of Thiocyanate in Human Saliva
PublicationA new solid-contact potentiometric ion-selective electrode for the determination of SCN− (SCN-ISE) has been described. Synthesized phosphonium derivative of calix[4]arene was used as a charged ionophore. The research included selection of the ion-selective membrane composition, determination of the ISEs metrological parameters and SCN-ISE application for thiocyanate determination in human saliva. Preparation of the ISEs included...
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Study of Aliphatic Polyurethanes by the Low-Field 1H NMR Relaxometry Method with the Inversion of the Integral Transformation
PublicationIn this paper, the distributions of the 1H nuclear magnetic resonance (NMR) spin–lattice and spin–spin relaxation times are used to characterize the mobility of different sections of macromolecules of aliphatic polyurethanes and the cross-linking density of polymer chains. The NMR relaxometry method with inversion of integral transformation is applied to study the effect of poly (ethylene glycol) and glycerol phosphate calcium...
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Imidazoacridinone-dependent lysosomal photodestruction: a pharmacological Trojan horse approach to eradicate multidrug-resistant cancers
PublicationMultidrug resistance (MDR) remains a primary hindrance to curative cancer therapy. Thus, introduction of novel strategies to overcome MDR is of paramount therapeutic significance. Sequestration of chemotherapeutics in lysosomes is an established mechanism of drug resistance. Here, we show that MDR cells display a marked increase in lysosome number. We further demonstrate that imidazoacridinones (IAs), which are cytotoxic fluorochromes,...